SU278032A1 - - Google Patents
Info
- Publication number
- SU278032A1 SU278032A1 SU1294010A SU1294010A SU278032A1 SU 278032 A1 SU278032 A1 SU 278032A1 SU 1294010 A SU1294010 A SU 1294010A SU 1294010 A SU1294010 A SU 1294010A SU 278032 A1 SU278032 A1 SU 278032A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- yeast
- medium
- aeration
- iron
- yield
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 10
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 8
- 238000005273 aeration Methods 0.000 description 5
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Chemical class [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical class N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102000022270 cytochrome c family Human genes 0.000 description 3
- 108091010617 cytochrome c family Proteins 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000589220 Acetobacter Species 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N Calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K Tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical class [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drugs Drugs 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 229910052500 inorganic mineral Chemical class 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 159000000014 iron salts Chemical class 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000011707 mineral Chemical class 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 206010007554 Cardiac failure Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 206010019280 Heart failure Diseases 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000011124 aluminium ammonium sulphate Nutrition 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- UMEAURNTRYCPNR-UHFFFAOYSA-O azanium;iron(2+) Chemical compound [NH4+].[Fe+2] UMEAURNTRYCPNR-UHFFFAOYSA-O 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000004098 cellular respiration Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002906 microbiologic Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000019798 tripotassium phosphate Nutrition 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Description
Изобретение относитс к области фармакологии , точнее к микробиологическим способам получени лекарственных препаратов, а именно цитохрома С, используемого дл лечени сердечной недостаточности и других болезней, св занных с затруднением клеточного дыхани . Известный способ получени цптохрома С путем культивировани дрожжей рода Candida в услови х аэрации па питательной среде, содержащей источники углерода, азота и мпнеральные соли, не обесиечивает удовлетворительного выхода питохрома С и весьма ие экономичен из-за необходимости использовани дефпцитиых компоиептов среды - витамина В|, глицина и а-кетоглутаровой кислоты. Дл удешевлени процесса и повышени выхода препарата предлагаетс культивирование дрожжей вида Candida mycoderma вести в присутствии дрожжевого автолизата и солей железа и в услови х иониженной аэрации (сульфитное число 0,6-0,8 г ОаЛ/час). Концентрацию солей железа в среде при культивировании ноддерживают на уровне 1-5 мг1л. Пример. Культуру Candida mycoderma ВКМ-У-934 поддерживают па сусло-агаре. Среда дл получени посевного материала п основного процесса выраш.ивани биомассы дрож/кей имеет следуюш,пй состав: 0,3% сульфата аммони , 0,07% сернокислого магни , 0,05% хлористого натри , 0,04% нитрата кальци , 0,01% фосфорнокислого кали двухзамешенного , 0,1% фосфорнокислого кали однозамещенпого , 2% глюкозы, 5% автолизата пивиых дрожжей и 0,003% железоаммиачных квасцов. Готов т среду на кип ченой водопроводной воде. Дрожжи выращивают в колбах на качалках ири 28-ЗО С в услови х умеренной аэрации (0,75 zQiAliac. Посевиой материал в возрасте 1 сутки виос т в оиытные колбы в количестве 2% от объема среды. Значеиие рН во врем основного процесса выращпвани дрожжей поддерживают па уровне 4,0-4,5 путем периодического введеии стерильного 10%-ного раствора едкого натра. Продолл ительпость культивировани двое суток. Выход биомассы дрожл ей - 6,8 г сухих клеток в 1 л среды. Содержание цитохрома С составл ет 3150 мг, на 1 кг сухих клеток. Предмет изобретени 1. Способ получени цитохрома С путем культивировани дрожжей рода Candida в услови х аэрации на питательной среде, содержащей источники углерода, азота и мине3 ральиые соли, отличающийс тем, что, с целью удешевлени нроцесса и повышени выхода нродукта, культивнроваиие дрожжей вида Candida mycoderma ведут в ирисутствии дрожжевого автолизата и солей железа и в уело-5 4 , ви х пониженной аэрации (сульфитное число 0,6-0,8 г ). 2. Сиособ но н. 1, отличающийс тем, что культивирование осуществл ют ири концеи трации железа в среде 1-5 мг/л.The invention relates to the field of pharmacology, more specifically to microbiological methods for the preparation of drugs, namely cytochrome C, used to treat heart failure and other diseases associated with the difficulty of cellular respiration. The known method of obtaining cytochrome C by cultivating the yeast genus Candida under conditions of aeration in a nutrient medium containing sources of carbon, nitrogen and mineral salts does not provide a satisfactory yield of nutritional C and is very economical due to the need to use environment components - vitamin B | glycine and a-ketoglutaric acid. To reduce the cost of the process and increase the yield of the drug, it is proposed to cultivate the yeast species Candida mycoderma in the presence of yeast autolysate and iron salts and under conditions of ionized aeration (sulfite value 0.6-0.8 g OL / h). The concentration of iron salts in the medium during cultivation is maintained at the level of 1-5 mg1l. Example. Culture of Candida mycoderma VKM-U-934 support pas wort agar. The medium for obtaining seed in the main process of growing biomass, the yeast / kei has the following composition: 0.3% ammonium sulfate, 0.07% magnesium sulphate, 0.05% sodium chloride, 0.04% calcium nitrate, 0 , 01% potassium phosphate dibasic, 0.1% potassium phosphate monosubstituted, 2% glucose, 5% brewer's yeast autolysate, and 0.003% iron ammonium alum. Prepare the medium on boiled tap water. The yeast is grown in flasks on iri-28-ZO C shakers under conditions of moderate aeration (0.75 zQiAliac. The sowing material at the age of 1 day is transferred to 2% of the volume of the medium in the flasks. The pH value during the main process of growing the yeast is maintained at a level of 4.0-4.5 by periodically injecting sterile 10% sodium hydroxide solution. It lasts for two days and cultivates two days. The biomass yield is 6.8 g dry cells per liter of medium. The cytochrome C content is 3150 mg per kg dry cells. Subject of the invention 1. Method of preparation Totochrome C by cultivating Candida's yeast under aeration conditions on a nutrient medium containing sources of carbon, nitrogen and minerals, characterized in that, in order to reduce the process and increase the yield of the product, the cultivation of the yeast Candida mycoderma species is conducted in the presence of yeast autolysate and salts of iron and in ulo-5 4, types of reduced aeration (sulfite number 0.6-0.8 g). 2. Particular n. 1, characterized in that the cultivation is carried out and iron ends in medium 1-5 mg / l.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU278032A1 true SU278032A1 (en) |
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