SU1039962A1 - Culture medium for culturing fungum eremothecium ashby ii producing riboflavin - Google Patents
Culture medium for culturing fungum eremothecium ashby ii producing riboflavin Download PDFInfo
- Publication number
- SU1039962A1 SU1039962A1 SU823440308A SU3440308A SU1039962A1 SU 1039962 A1 SU1039962 A1 SU 1039962A1 SU 823440308 A SU823440308 A SU 823440308A SU 3440308 A SU3440308 A SU 3440308A SU 1039962 A1 SU1039962 A1 SU 1039962A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- riboflavin
- ashby
- eremothecium
- fungum
- culturing
- Prior art date
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- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 title claims description 19
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 title claims description 10
- 229960002477 riboflavin Drugs 0.000 title claims description 10
- 235000019192 riboflavin Nutrition 0.000 title claims description 10
- 239000002151 riboflavin Substances 0.000 title claims description 10
- 241001465321 Eremothecium Species 0.000 title description 2
- 238000012258 culturing Methods 0.000 title 1
- 239000001963 growth medium Substances 0.000 title 1
- FBEHFRAORPEGFH-UHFFFAOYSA-N Allyxycarb Chemical compound CNC(=O)OC1=CC(C)=C(N(CC=C)CC=C)C(C)=C1 FBEHFRAORPEGFH-UHFFFAOYSA-N 0.000 claims description 11
- 239000010802 sludge Substances 0.000 claims description 9
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000002028 Biomass Substances 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 239000008399 tap water Substances 0.000 claims description 2
- 235000020679 tap water Nutrition 0.000 claims description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims 1
- 235000013305 food Nutrition 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 235000002639 sodium chloride Nutrition 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 235000021321 essential mineral Nutrition 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000009428 plumbing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
0000
со со о 1Cwith with about 1C
Изобретение относитс к микробиологической промышленности, а именно к питательной среде дл выращивани продуцента рибофлавина.The invention relates to the microbiological industry, namely to a nutrient medium for growing the producer of riboflavin.
Известна питательна среда дл выращивани гриба Eremothecium ashbyll - продуцента рибофлавина, в состав которой вход т отходы переработки сем н масличных культур, кукурузный экстракт, гидрол, сахар, солод, мел химический, дрожжи, соль поваренна , сернокислый магний, сернокислое железо, микроэлементы, жир технический и вода,(1.Known nutrient medium for the cultivation of the fungus Eremothecium ashbyll - producer of riboflavin, which includes waste processing of oilseeds, corn extract, hydrol, sugar, malt, chemical chalk, yeast, salt, magnesium sulphate, iron sulfate, trace elements, fat technical and water, (1.
Недостатком данной среды вл етс низка активность рибофлавина, котора составл ет в среднем ЙОО мкг/мл, кроме того, эта среда дорогосто ща , так как содержит двенадцать компонентов, два из которых вл ютс пищевыми (саХар и поваренна соль).The disadvantage of this medium is low riboflavin activity, which averages HLR µg / ml, in addition, this medium is expensive because it contains twelve components, two of which are edible (saChar and sodium chloride).
Известно использование сухого активного ила в качестве питательной среды дл выращивани бактерий Bacillus thurIngiersus, которь1й вл етс источником азота, витаминов и минерального питани {.,It is known to use dry activated sludge as a nutrient medium for growing bacteria Bacillus thurIngiersus, which is a source of nitrogen, vitamins and mineral nutrition {.,
Наиболее близкой к изобретению по составу вл етс среда дл культивировани гриба Ereinothec ium ashbyH, продуцирующего, рибофлавин, включающа соевую муку и кукурузный экстракт в качестве источника азота и фосфора, солод, мел химический., поваренную соль, дрожжи сухие, сахарозу и гидрол в качестве источника углерода и необходимые минеральные соли,используема в промышленности как регламентна питательна среда 3j.The closest to the invention in composition is the medium for cultivation of the fungus Ereinothec ium ashbyH, producing riboflavin, including soy flour and corn extract as a source of nitrogen and phosphorus, malt, chemical chalk, table salt, dry yeast, sucrose and hydrol as carbon source and essential mineral salts used in industry as a regulatory nutrient medium 3j.
Недостатком известной среды вл етс низка активность рибофлавина, составл юща 1000-1200 мкг/мл. A disadvantage of the known medium is low riboflavin activity of 1000-1200 µg / ml.
Целью изобретени вл етс повышение выхода рибофлавина и удешевление среды.The aim of the invention is to increase the yield of riboflavin and reduce the cost of the medium.
Поставленна цель достигаетс тем, что питательна среда дл выращивани гриба Ег. ashbytl - продуцента рибофлавина, содержаща гидрол , мел химический и воду водопррводную , дополнительно содержит биомассу сухого активного ила или его серно-кислотный гидролизат при следующем соотношении компонентов, мзс. ТОО мл воды:The goal is achieved in that the nutrient medium for growing the fungus Er. ashbytl - riboflavin producer, containing hydrol, chemical chalk and water, also contains dry activated sludge biomass or its sulfuric acid hydrolyzate in the following ratio of components. Ml of water LLP:
Гидрол 3,0-6,0Hydrol 3.0-6.0
Мел химический0 ,5-0,6Chalk chemical0, 5-0,6
Биомасса сухого активного ила или его серно-кислотный гидролизат1,0-,0Biomass of dry activated sludge or its sulfuric acid hydrolyzate1.0-, 0
. Гидрол, вход щий а состав питатеной среды, полумают как отходы при производстве кристаллической глюкозы . По химическому составу гидрол содержит глхкозу, фосфор и поваренную соль, а дл культивировани Ег. ashbyi наилучшим источником углерода вл етс глюкоза. В св зи с этим сахар, вход щий в состав регламентной питательной среды, может быть заменен на гидрол, а поваренна сол исключена из нее.. The hydrol entering the composition of the nutrient medium is half out as waste in the production of crystalline glucose. By chemical composition, hydrol contains glhkoza, phosphorus and salt, and for the cultivation of Er. ashbyi the best carbon source is glucose. In this connection, the sugar, which is part of the regulatory nutrient medium, can be replaced by hydrol, and cooking salt is excluded from it.
В производственном отношении сухой активный ил вл етс удобным сырьем: он накапливаетс в аэротенках в процессе биологической очистки жиких отходов, получаемых при производстве сухих кормовых дрожжей (БВК la также при производстве органических растворителей.In terms of production, dry activated sludge is a convenient raw material: it accumulates in aeration tanks during the biological treatment of wild waste produced in the production of dry fodder yeast (BVK la also in the production of organic solvents.
Сухой активный ил, получаемый на заводах кормовых дрожжей и органческих растворителей, по СБОИМ питательным качествам обладает ценными свойствами:содержит 50-57,0% сырого протеина, витамины и минеральные .элементы. Таким образом, сухой активный ил вл етс источником азота, витаминов и минеральiHoro питани дл микроорганизмов.Dry activated sludge, obtained at plants of fodder yeast and organic solvents, by SOBIM nutritional qualities, has valuable properties: it contains 50-57.0% crude protein, vitamins and mineral elements. Thus, dry activated sludge is a source of nitrogen, vitamins and mineral nutrition for microorganisms.
Пример 1. Состав примен е .мой среды, мас.%: Сухой активный ил1,0Example 1. The composition of the application. My environment, wt.%: Dry active or 1.0
Гидрол.3,0Hydrol.3,0
Мел химический0,5Chalk chemical0.5
Вода водопроводна - до 100 мл; рН после стерили . зации6,6-6,,8Piped water - up to 100 ml; pH after sterilization. zation6,6-6,, 8
Приготовление среды дл ферментеров ведетс в специальных чанах-смесител х , установленных в отделении дл приготовлени питательных сред. Среду готов т в две партии. Состав ферментационной среды: 1 парти сухой активный ил, мел химический и вода водопрбводча ; 2 парти гидрол , вода водопроводна .The preparation of the medium for the fermenters is carried out in special mixing tanks set up in the compartment for the preparation of nutrient media. The medium is prepared in two batches. The composition of the fermentation medium: 1 batch of dry activated sludge, chemical chalk and water vodrbvodcha; 2 party hydrol, water plumbing.
Первую napTVflo среды готое т в чане-смесителе 15 м, оборудованном мешалкой и барботером дл подачи пара.Набирают м водопроводной воды, загружают сухой активный f/лThe first napTVflo of the medium is goto t in a 15 m mixing tank equipped with a stirrer and a bubbler to supply steam. Type m tap water, load dry active f / l
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU823440308A SU1039962A1 (en) | 1982-05-19 | 1982-05-19 | Culture medium for culturing fungum eremothecium ashby ii producing riboflavin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU823440308A SU1039962A1 (en) | 1982-05-19 | 1982-05-19 | Culture medium for culturing fungum eremothecium ashby ii producing riboflavin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1039962A1 true SU1039962A1 (en) | 1983-09-07 |
Family
ID=21012508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU823440308A SU1039962A1 (en) | 1982-05-19 | 1982-05-19 | Culture medium for culturing fungum eremothecium ashby ii producing riboflavin |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1039962A1 (en) |
-
1982
- 1982-05-19 SU SU823440308A patent/SU1039962A1/en active
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