SK71596A3 - Non-enzymatic method of determination of reducing sugars content in aqueous sulution - Google Patents

Non-enzymatic method of determination of reducing sugars content in aqueous sulution Download PDF

Info

Publication number: SK71596A3
Authority: SK; Slovakia
Prior art keywords: electrode; hydroxide; reducing sugars; cfd; working electrode
Prior art date: 1993-12-08

Application number

SK715-96A

Other languages

English (en)

Slovak (sk)

Inventor

Brian J Birch

Vincent Bonnafoux

Nicholas A Morris

Original Assignee

Unilever Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-12-08

Filing date

1994-12-05

Publication date

1997-06-04

1994-12-05 Application filed by Unilever Nv filed Critical Unilever Nv

1997-06-04 Publication of SK71596A3 publication Critical patent/SK71596A3/sk

Links

235000000346 sugar Nutrition 0.000 title claims abstract description 83
150000008163 sugars Chemical class 0.000 title claims abstract description 43
230000001603 reducing effect Effects 0.000 title claims abstract description 40
238000006911 enzymatic reaction Methods 0.000 title claims 2
238000000034 method Methods 0.000 claims abstract description 60
WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 claims abstract description 49
239000007864 aqueous solution Substances 0.000 claims abstract description 12
239000012047 saturated solution Substances 0.000 claims abstract description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 27
239000007788 liquid Substances 0.000 claims description 23
230000001681 protective effect Effects 0.000 claims description 20
239000004460 silage Substances 0.000 claims description 16
238000006243 chemical reaction Methods 0.000 claims description 14
239000000126 substance Substances 0.000 claims description 14
239000010409 thin film Substances 0.000 claims description 12
239000012530 fluid Substances 0.000 claims description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims description 7
230000009467 reduction Effects 0.000 claims description 7
150000008044 alkali metal hydroxides Chemical class 0.000 claims description 6
239000012085 test solution Substances 0.000 claims description 6
108090000790 Enzymes Proteins 0.000 claims description 4
102000004190 Enzymes Human genes 0.000 claims description 4
239000011248 coating agent Substances 0.000 claims description 2
238000000576 coating method Methods 0.000 claims description 2
239000007795 chemical reaction product Substances 0.000 claims 1
239000000047 product Substances 0.000 claims 1
-1 potassium ferricyanide Chemical compound 0.000 abstract description 16
229930091371 Fructose Natural products 0.000 description 31
239000005715 Fructose Substances 0.000 description 31
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 31
238000005259 measurement Methods 0.000 description 26
239000000523 sample Substances 0.000 description 25
239000000243 solution Substances 0.000 description 24
230000004044 response Effects 0.000 description 18
238000012360 testing method Methods 0.000 description 17
239000003153 chemical reaction reagent Substances 0.000 description 16
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 14
239000008103 glucose Substances 0.000 description 14
UETZVSHORCDDTH-UHFFFAOYSA-N iron(2+);hexacyanide Chemical compound [Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] UETZVSHORCDDTH-UHFFFAOYSA-N 0.000 description 14
238000003869 coulometry Methods 0.000 description 13
150000002500 ions Chemical class 0.000 description 13
238000002474 experimental method Methods 0.000 description 12
230000002829 reductive effect Effects 0.000 description 11
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 10
239000010931 gold Substances 0.000 description 10
229910052737 gold Inorganic materials 0.000 description 10
KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 9
230000000694 effects Effects 0.000 description 9
239000003795 chemical substances by application Substances 0.000 description 6
238000007254 oxidation reaction Methods 0.000 description 6
238000006722 reduction reaction Methods 0.000 description 6
238000007792 addition Methods 0.000 description 5
239000008280 blood Substances 0.000 description 5
210000004369 blood Anatomy 0.000 description 5
238000000691 measurement method Methods 0.000 description 5
230000003647 oxidation Effects 0.000 description 5
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
230000008901 benefit Effects 0.000 description 4
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
229910052799 carbon Inorganic materials 0.000 description 4
230000008859 change Effects 0.000 description 4
238000007650 screen-printing Methods 0.000 description 4
238000003860 storage Methods 0.000 description 4
230000002411 adverse Effects 0.000 description 3
239000012491 analyte Substances 0.000 description 3
238000003556 assay Methods 0.000 description 3
239000013060 biological fluid Substances 0.000 description 3
238000001514 detection method Methods 0.000 description 3
238000009792 diffusion process Methods 0.000 description 3
229940088598 enzyme Drugs 0.000 description 3
YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 3
229930182830 galactose Natural products 0.000 description 3
DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 description 3
235000016709 nutrition Nutrition 0.000 description 3
230000010287 polarization Effects 0.000 description 3
230000035484 reaction time Effects 0.000 description 3
238000002798 spectrophotometry method Methods 0.000 description 3
SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
241000196324 Embryophyta Species 0.000 description 2
108010015776 Glucose oxidase Proteins 0.000 description 2
239000004366 Glucose oxidase Substances 0.000 description 2
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
239000003513 alkali Substances 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
235000013361 beverage Nutrition 0.000 description 2
239000012482 calibration solution Substances 0.000 description 2
239000000919 ceramic Substances 0.000 description 2
230000007423 decrease Effects 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
235000013399 edible fruits Nutrition 0.000 description 2
238000002848 electrochemical method Methods 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
235000013305 food Nutrition 0.000 description 2
239000011521 glass Substances 0.000 description 2
229940116332 glucose oxidase Drugs 0.000 description 2
235000019420 glucose oxidase Nutrition 0.000 description 2
238000011065 in-situ storage Methods 0.000 description 2
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
239000000463 material Substances 0.000 description 2
239000000203 mixture Substances 0.000 description 2
238000012544 monitoring process Methods 0.000 description 2
229910052709 silver Inorganic materials 0.000 description 2
239000004332 silver Substances 0.000 description 2
239000011550 stock solution Substances 0.000 description 2
235000013311 vegetables Nutrition 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
PJVXUVWGSCCGHT-ZPYZYFCMSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;(3s,4r,5r)-1,3,4,5,6-pentahydroxyhexan-2-one Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O.OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO PJVXUVWGSCCGHT-ZPYZYFCMSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
238000008458 Glucose Oxidase Reagent Methods 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
229910002651 NO3 Inorganic materials 0.000 description 1
240000002853 Nelumbo nucifera Species 0.000 description 1
235000006508 Nelumbo nucifera Nutrition 0.000 description 1
235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
229910021607 Silver chloride Inorganic materials 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
150000001447 alkali salts Chemical class 0.000 description 1
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
239000011230 binding agent Substances 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
239000003054 catalyst Substances 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
229910010293 ceramic material Inorganic materials 0.000 description 1
238000013375 chromatographic separation Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
239000004020 conductor Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
239000013078 crystal Substances 0.000 description 1
238000002484 cyclic voltammetry Methods 0.000 description 1
239000002274 desiccant Substances 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
238000010586 diagram Methods 0.000 description 1
150000002016 disaccharides Chemical class 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000000835 electrochemical detection Methods 0.000 description 1
238000003487 electrochemical reaction Methods 0.000 description 1
230000005518 electrochemistry Effects 0.000 description 1
239000007772 electrode material Substances 0.000 description 1
238000010828 elution Methods 0.000 description 1
238000009313 farming Methods 0.000 description 1
229940073322 ferric hexacyanoferrate Drugs 0.000 description 1
229910001447 ferric ion Inorganic materials 0.000 description 1
150000002231 fructose derivatives Chemical class 0.000 description 1
150000002402 hexoses Chemical class 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
238000009434 installation Methods 0.000 description 1
239000004310 lactic acid Substances 0.000 description 1
235000014655 lactic acid Nutrition 0.000 description 1
239000011159 matrix material Substances 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
229910000000 metal hydroxide Inorganic materials 0.000 description 1
150000004692 metal hydroxides Chemical class 0.000 description 1
238000002156 mixing Methods 0.000 description 1
150000002772 monosaccharides Chemical class 0.000 description 1
229910052759 nickel Inorganic materials 0.000 description 1
230000001590 oxidative effect Effects 0.000 description 1
238000001139 pH measurement Methods 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
239000004417 polycarbonate Substances 0.000 description 1
229920000515 polycarbonate Polymers 0.000 description 1
239000000276 potassium ferrocyanide Substances 0.000 description 1
239000000376 reactant Substances 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
238000006479 redox reaction Methods 0.000 description 1
238000010405 reoxidation reaction Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
AWDBHOZBRXWRKS-UHFFFAOYSA-N tetrapotassium;iron(6+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+6].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] AWDBHOZBRXWRKS-UHFFFAOYSA-N 0.000 description 1
230000007704 transition Effects 0.000 description 1
238000004832 voltammetry Methods 0.000 description 1
238000007704 wet chemistry method Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Chemical Kinetics & Catalysis (AREA)
Electrochemistry (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Immunology (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Secondary Cells (AREA)

SK715-96A 1993-12-08 1994-12-05 Non-enzymatic method of determination of reducing sugars content in aqueous sulution SK71596A3 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB939325189A GB9325189D0 (en)	1993-12-08	1993-12-08	Methods and apparatus for electrochemical measurements
PCT/GB1994/002656 WO1995016198A1 (en)	1993-12-08	1994-12-05	Methods and apparatus for electrochemical measurements

Publications (1)

Publication Number	Publication Date
SK71596A3 true SK71596A3 (en)	1997-06-04

Family

ID=10746333

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK715-96A SK71596A3 (en)	1993-12-08	1994-12-05	Non-enzymatic method of determination of reducing sugars content in aqueous sulution

Country Status (14)

Country	Link
US (1)	US5645709A (pt)
EP (1)	EP0734520A1 (pt)
JP (1)	JPH09500450A (pt)
CN (1)	CN1142867A (pt)
AU (1)	AU1116795A (pt)
BR (1)	BR9408250A (pt)
CA (1)	CA2178626A1 (pt)
GB (1)	GB9325189D0 (pt)
HU (1)	HUT76409A (pt)
PL (1)	PL314911A1 (pt)
SK (1)	SK71596A3 (pt)
TR (1)	TR28318A (pt)
WO (1)	WO1995016198A1 (pt)
ZA (1)	ZA949754B (pt)

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1993
- 1993-12-08 GB GB939325189A patent/GB9325189D0/en active Pending
1994
- 1994-12-05 SK SK715-96A patent/SK71596A3/sk unknown
- 1994-12-05 AU AU11167/95A patent/AU1116795A/en not_active Abandoned
- 1994-12-05 WO PCT/GB1994/002656 patent/WO1995016198A1/en not_active Application Discontinuation
- 1994-12-05 PL PL94314911A patent/PL314911A1/xx unknown
- 1994-12-05 EP EP95902232A patent/EP0734520A1/en not_active Withdrawn
- 1994-12-05 JP JP7516030A patent/JPH09500450A/ja active Pending
- 1994-12-05 BR BR9408250A patent/BR9408250A/pt not_active Application Discontinuation
- 1994-12-05 CA CA002178626A patent/CA2178626A1/en not_active Abandoned
- 1994-12-05 CN CN94194934A patent/CN1142867A/zh active Pending
- 1994-12-05 HU HU9601575A patent/HUT76409A/hu unknown
- 1994-12-07 US US08/350,862 patent/US5645709A/en not_active Expired - Fee Related
- 1994-12-07 ZA ZA949754A patent/ZA949754B/xx unknown
- 1994-12-08 TR TR01284/94A patent/TR28318A/xx unknown

Also Published As

Publication number	Publication date
EP0734520A1 (en)	1996-10-02
GB9325189D0 (en)	1994-02-09
US5645709A (en)	1997-07-08
JPH09500450A (ja)	1997-01-14
PL314911A1 (en)	1996-09-30
AU1116795A (en)	1995-06-27
CN1142867A (zh)	1997-02-12
HUT76409A (en)	1997-08-28
TR28318A (tr)	1996-04-24
WO1995016198A1 (en)	1995-06-15
HU9601575D0 (en)	1996-07-29
CA2178626A1 (en)	1995-06-15
BR9408250A (pt)	1997-05-27
ZA949754B (en)	1996-06-07

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