SI2731973T1 - Bakterijski gostiteljski sev, ki izraža rekombinantni DSBC - Google Patents
Bakterijski gostiteljski sev, ki izraža rekombinantni DSBC Download PDFInfo
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- SI2731973T1 SI2731973T1 SI201231206T SI201231206T SI2731973T1 SI 2731973 T1 SI2731973 T1 SI 2731973T1 SI 201231206 T SI201231206 T SI 201231206T SI 201231206 T SI201231206 T SI 201231206T SI 2731973 T1 SI2731973 T1 SI 2731973T1
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- 230000001580 bacterial effect Effects 0.000 title claims abstract 19
- 102000040430 polynucleotide Human genes 0.000 claims abstract 14
- 108091033319 polynucleotide Proteins 0.000 claims abstract 14
- 239000002157 polynucleotide Substances 0.000 claims abstract 14
- 239000012634 fragment Substances 0.000 claims abstract 12
- 239000000427 antigen Substances 0.000 claims abstract 10
- 102000036639 antigens Human genes 0.000 claims abstract 10
- 108091007433 antigens Proteins 0.000 claims abstract 10
- 239000013604 expression vector Substances 0.000 claims abstract 7
- 210000004027 cell Anatomy 0.000 claims 16
- 238000000034 method Methods 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 235000001014 amino acid Nutrition 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 235000018102 proteins Nutrition 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 210000004602 germ cell Anatomy 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 229920001427 mPEG Polymers 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 241001646716 Escherichia coli K-12 Species 0.000 claims 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 1
- 206010064571 Gene mutation Diseases 0.000 claims 1
- 101150052672 IGS1 gene Proteins 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 235000004279 alanine Nutrition 0.000 claims 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 1
- 239000012636 effector Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 210000001322 periplasm Anatomy 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2875—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF/TNF superfamily, e.g. CD70, CD95L, CD153, CD154
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Claims (15)
- Bakterijski gostiteljski sev, ki izraža rekombinantni DsbC Patentni zahtevki1. Rekombinantna gramnegativna bakterijska celica, ki obsega: a) ekspresijski vektor, ki obsega rekombinantni polinukleotid, ki kodira DsbC; in b) enega ali več polinukleotidov, ki kodirajo protitelo ali njegov antigen-vezavni fragment, ki se specifično veže na CD154; c) mutiran gen Tsp, ki kodira beljakovino Tsp, ki ima 50 % ali manj proteazne aktivnosti nemutirane beljakovine Tsp divjega tipa, kot je prikazana na SEQ ID NO: 26 in; d) mutiran gen spr, ki kodira beljakovino spr, katere zaporedje divjega tipa je prikazano na SEQ ID NO: 24, ki obsega mutacijo, ki zadeva aminokislino C94; in pri čemer je gramnegativna bakterijska celica izbrana iz sevov E. coli K12 in VV3110 in pri čemer je bakterijska celica izogena omenjenim sevom, razen rekombinantnega polinukleotida, ki kodira DsbC, pri čemer en ali več polinukleotidov kodira protitelo ali njegov antigen-vezavni fragment, ki se specifično veže na CD154 in mutirani gen Tsp in mutirani gen spr;
- 2. Gramnegativna bakterijska celica po zahtevku 1, pri čemer DsbC obsega histidinski označevalec na N-koncu ali C-koncu.
- 3. Gramnegativna bakterijska celica po zahtevku 1 ali 2, pri čemer ekspresijski vektor obsega polinukleotid, ki ima zaporedje, podano v SEQ ID NO:45 ali SEQ ID NO:51.
- 4. Gramnegativna bakterijska celica po katerem koli od zahtevkov od 1 do 3, pri čemer protitelo ali njegov antigen-vezavni fragment obsega variabilno domeno težke verige, ki obsega tri CDR-je, ki imajo zaporedje podano v SEQ ID NO: 1 za CDRH1, SEQ ID NO: 2, za CDRH2 in SEQ ID NO: 3 za CDRH3 in variabilno domeno lahke verige, ki obsega tri CDR-je, ki imajo zaporedje podano v SEQ ID NO: 4 za CDRL1, SEQ ID NO: 5 za CDRL2 in SEQ ID NO: 6 za CDRL3.
- 5. Gramnegativna bakterijska celica po zahtevku 4, pri čemer en ali več polinukleotidov kodira protitelo, ki obsega zaporedje variabilne regije lahke verige, ki je podano v SEQ ID NO:8, in variabilno regijo težke verige, ki je podana v SEQ ID NO:10.
- 6. Gramnegativna bakterijska celica po katerem koli od zahtevkov od 1 do 5, pri čemer je protitelo fragment Fab ali Fab‘.
- 7. Gramnegativna bakterijska celica po zahtevku 6, pri čemer fragment Fab ali Fab‘ obsega lahko verigo, ki ima zaporedje, podano v SEQ ID NO: 12 in težko verigo, ki ima zaporedje, podano v SEQ ID NO:14 ali 16.
- 8. Gramnegativna bakterijska celica po katerem koli od zahtevkov od 1 do 7, pri čemer gramnegativna bakterijska celica obsega prvi ekspresijski vektor, ki obsega rekombinantni polinukleotid, ki kodira DsbC, in drugi ekspresijski vektor, ki obsega enega ali več polinukleotidov, ki kodirajo protitelo ali njegov antigen-vezavni fragment, ki se specifično veže na CD154.
- 9. Gramnegativna bakterijska celica po katerem koli od zahtevkov od 1 do 7, pri čemer ekspresijski vektor, ki obsega rekombinantni polinukleotid, ki kodira DsbC, dodatno obsega enega ali več polinukleotidov, ki kodirajo protitelo ali njegov antigen-vezavni fragment, ki se specifično veže na CD154.
- 10. Gramnegativna bakterijska celica po katerem koli predhodnem zahtevku, pri čemer mutacija v genu spr, ki kodira beljakovino spr, privede do spremembe aminokisline cistein v alanin na položaju 94 (C94A).
- 11. Gramnegativna bakterijska celica po katerem koli od zahtevkov od 1 do 10, ki obsega ekspresijski vektor, ki obsega rekombinantni polinukleotid, ki kodira DsbC in dicistronsko sporočilo za tvorbo protitelesa ali njegovega antigen-vezavnega fragmenta, ki se specifično veže na CD154, pri čemer zgornji cistron vsebuje DNA, ki kodira lahko verigo protitelesa, in spodnji cistron vsebuje DNA, ki kodira ustrezno težko verigo, označena s tem, da dicistronsko sporočilo obsega zaporedje, izbrano izmed IGS1 (SEQ ID NO: 33), IGS2 (SEQ ID NO: 34), IGS3 (SEQ ID NO: 35) in IGS4 (SEQ ID NO: 36).
- 12. Postopek za tvorbo protitelesa ali njegovega antigen-vezavnega fragmenta, ki se specifično veže na CD154, pri čemer postopek obsega: a) gojenje rekombinantne gramnegativne bakterijske celice, kot je definirana v katerem koli od zahtevkov od 1 do 11, v gojišču v pogojih, ki so učinkoviti za izražanje protitelesa ali njegovega antigen-vezavnega fragmenta, ki se specifično veže na CD154, in rekombinantnega polinukleotida, ki kodira DsbC; in b) pridobivanje protitelesa ali njegovega antigen-vezavnega fragmenta, ki se specifično veže na CD154, iz periplazme rekombinantne gramnegativne bakterijske celice in/ali gojišča.
- 13. Postopek po zahtevku 12, pri čemer postopek dalje obsega korak pritrjanja efektorske molekule na aminokislino pri ali proti C-koncu težke verige in/ali lahke verige protitelesa.
- 14. Postopek po zahtevku 13, pri čemer efektorska molekula obsega poli(etilenglikol) ali metoksipoli(etilenglikol).
- 15. Postopek po zahtevku 14, pri čemer postopek obsega pritrjanje lizil-maleimidne skupine na enem od cisteinskih ostankov pri C-koncu težke verige, pri čemer ima vsaka amino skupina lizilnega ostanka nase kovalentno vezan metoksipoli(etilenglikolni) ostanek, ki ima molekulsko maso okoli 20.000 Da.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11173880 | 2011-07-13 | ||
| EP12737492.4A EP2731973B1 (en) | 2011-07-13 | 2012-07-13 | Bacterial host strain expressing recombinant dsbc |
| PCT/EP2012/002945 WO2013007388A1 (en) | 2011-07-13 | 2012-07-13 | Bacterial host strain expressing recombinant dsbc |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SI2731973T1 true SI2731973T1 (sl) | 2018-04-30 |
Family
ID=46545736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI201231206T SI2731973T1 (sl) | 2011-07-13 | 2012-07-13 | Bakterijski gostiteljski sev, ki izraža rekombinantni DSBC |
Country Status (27)
| Country | Link |
|---|---|
| US (2) | US9725516B2 (sl) |
| EP (2) | EP2731973B1 (sl) |
| JP (3) | JP6431370B2 (sl) |
| KR (1) | KR102023786B1 (sl) |
| CN (1) | CN103649124B (sl) |
| AU (1) | AU2012283388B2 (sl) |
| BR (1) | BR112013032871B1 (sl) |
| CA (1) | CA2841824C (sl) |
| CY (1) | CY1119985T1 (sl) |
| DK (1) | DK2731973T3 (sl) |
| EA (1) | EA031449B1 (sl) |
| ES (1) | ES2659155T3 (sl) |
| HR (1) | HRP20180226T1 (sl) |
| HU (1) | HUE035674T2 (sl) |
| IL (1) | IL229947B (sl) |
| IN (1) | IN2014DN00202A (sl) |
| LT (1) | LT2731973T (sl) |
| ME (1) | ME02957B (sl) |
| MX (1) | MX348738B (sl) |
| NO (1) | NO2731973T3 (sl) |
| PL (1) | PL2731973T3 (sl) |
| PT (1) | PT2731973T (sl) |
| RS (1) | RS56926B1 (sl) |
| SG (1) | SG10201807560XA (sl) |
| SI (1) | SI2731973T1 (sl) |
| SM (1) | SMT201800068T1 (sl) |
| WO (1) | WO2013007388A1 (sl) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0809042B1 (pt) * | 2007-03-22 | 2021-08-31 | Biogen Ma Inc. | Proteína de ligação a cd154 isolada, seu uso, e composição |
| HUE040306T2 (hu) | 2009-09-24 | 2019-03-28 | Ucb Biopharma Sprl | Baktériumtörzs rekombináns fehérje expresszálására, amely tartalmaz proteázhiányos, de chaperonaktivitását megtartott DEGP-t és génkiütött TSP és PTR gént |
| GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000591D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201012599D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Process for purifying proteins |
| EA031449B1 (ru) * | 2011-07-13 | 2019-01-31 | Юсб Фарма, С.А. | Бактериальный штамм-акцептор, экспрессирующий рекомбинантный dsbc |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
| GB201223276D0 (en) * | 2012-12-21 | 2013-02-06 | Ucb Pharma Sa | Antibodies and methods of producing same |
| US10358460B2 (en) | 2014-12-22 | 2019-07-23 | Ucb Biopharma Sprl | Protein manufacture |
| KR101810778B1 (ko) * | 2015-06-23 | 2017-12-20 | 서울대학교산학협력단 | Cd154 결합 폴리펩타이드 및 그 용도 |
| BR112018015259A2 (pt) | 2016-01-27 | 2018-12-18 | Medimmune Llc | métodos para preparação de anticorpos com um padrão de glicosilação definido |
| WO2019193418A1 (en) | 2018-04-05 | 2019-10-10 | Bio-Rad Abd Serotec Gmbh | Display systems for proteins of interest |
| SG11202104104VA (en) * | 2018-11-05 | 2021-05-28 | Genentech Inc | Methods of producing two chain proteins in prokaryotic host cells |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| EP3942079A1 (en) | 2019-03-18 | 2022-01-26 | Bio-Rad ABD Serotec GmbH | Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation |
| PL4010500T3 (pl) * | 2019-08-05 | 2025-06-23 | Wacker Chemie Ag | Szczep bakteryjny do uwalniania białka rekombinowanego w sposobie fermentacji |
Family Cites Families (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
| GB8907617D0 (en) | 1989-04-05 | 1989-05-17 | Celltech Ltd | Drug delivery system |
| US5508192A (en) | 1990-11-09 | 1996-04-16 | Board Of Regents, The University Of Texas System | Bacterial host strains for producing proteolytically sensitive polypeptides |
| US5264365A (en) | 1990-11-09 | 1993-11-23 | Board Of Regents, The University Of Texas System | Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides |
| GB9112536D0 (en) | 1991-06-11 | 1991-07-31 | Celltech Ltd | Chemical compounds |
| US5474771A (en) | 1991-11-15 | 1995-12-12 | The Trustees Of Columbia University In The City Of New York | Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same |
| AU4118393A (en) | 1992-04-28 | 1993-11-29 | Regents Of The University Of Michigan, The | Human crabp-I and crabp-II |
| GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| US5304472A (en) | 1992-11-20 | 1994-04-19 | Genentech, Inc. | Method of controlling polypeptide production in bacterial cells |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| AU2660397A (en) | 1996-04-05 | 1997-10-29 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
| GB9625640D0 (en) | 1996-12-10 | 1997-01-29 | Celltech Therapeutics Ltd | Biological products |
| US6083715A (en) | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| JP2000083670A (ja) | 1998-09-09 | 2000-03-28 | Hsp Kenkyusho:Kk | ＤｓｂＡ／ＤｓｂＢ／ＤｓｂＣ／ＤｓｂＤ発現プラスミド |
| CA2377491C (en) | 1999-06-29 | 2008-01-29 | Siga Technologies, Inc. | Assay for degp protease inhibitors |
| GB0006398D0 (en) | 2000-03-16 | 2000-05-03 | Novartis Ag | Organic compounds |
| GB0013810D0 (en) | 2000-06-06 | 2000-07-26 | Celltech Chiroscience Ltd | Biological products |
| AU8867501A (en) | 2000-09-01 | 2002-03-13 | Biogen Inc | Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design |
| EP1314037A2 (en) | 2000-09-01 | 2003-05-28 | Biogen, Inc. | Methods of designing and producing compounds having improved binding affinity for cd154 or other trimeric proteins |
| US7041479B2 (en) | 2000-09-06 | 2006-05-09 | The Board Of Trustess Of The Leland Stanford Junior University | Enhanced in vitro synthesis of active proteins containing disulfide bonds |
| DK1341899T3 (da) | 2000-12-14 | 2006-04-10 | Genentech Inc | Bakterieværtsstammer |
| CA2430182C (en) * | 2000-12-14 | 2011-01-25 | Genentech, Inc. | Prokaryotically produced antibodies and uses thereof |
| WO2004081026A2 (en) | 2003-06-30 | 2004-09-23 | Domantis Limited | Polypeptides |
| TWI327597B (en) | 2001-08-01 | 2010-07-21 | Centocor Inc | Anti-tnf antibodies, compositions, methods and uses |
| DE60224291T2 (de) * | 2001-08-27 | 2008-12-11 | Genentech, Inc., South San Francisco | System zur antikörperexpression und- synthese |
| GB0124317D0 (en) | 2001-10-10 | 2001-11-28 | Celltech R&D Ltd | Biological products |
| IL161988A0 (en) | 2001-11-16 | 2005-11-20 | Corporation Idec Pharmaceutica | Polycistronic expression of antibodies |
| GB0129105D0 (en) * | 2001-12-05 | 2002-01-23 | Celltech R&D Ltd | Expression control using variable intergenic sequences |
| AU2003244817B2 (en) | 2002-06-28 | 2010-08-26 | Domantis Limited | Antigen-binding immunoglobulin single variable domains and dual-specific constructs |
| EP1578447A4 (en) | 2002-10-31 | 2009-06-03 | Genentech Inc | METHOD AND COMPOSITIONS FOR INCREASING ANTIBODY PRODUCTION |
| PT1570267E (pt) | 2002-12-03 | 2012-01-03 | Ucb Pharma Sa | Ensaio para a identificação de células produtoras de anticorpos |
| GB0303337D0 (en) | 2003-02-13 | 2003-03-19 | Celltech R&D Ltd | Biological products |
| CA2528551A1 (en) | 2003-06-13 | 2005-01-13 | Biogen Idec Ma Inc. | Aglycosyl anti-cd154 (cd40 ligand) antibodies and uses thereof |
| GB0315457D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
| GB0315450D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
| AU2004261198A1 (en) | 2003-07-26 | 2005-02-10 | Biogen Idec Ma Inc. | Altered antibodies having improved antigen-binding affinity |
| JP5102028B2 (ja) | 2004-07-26 | 2012-12-19 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | 抗ｃｄ１５４抗体 |
| US20060204493A1 (en) | 2004-09-02 | 2006-09-14 | Genentech, Inc. | Heteromultimeric molecules |
| US7563443B2 (en) | 2004-09-17 | 2009-07-21 | Domantis Limited | Monovalent anti-CD40L antibody polypeptides and compositions thereof |
| GB0425534D0 (en) | 2004-11-19 | 2004-12-22 | Celltech R&D Ltd | Process for obtaining antibodies |
| GB0520169D0 (en) | 2005-10-04 | 2005-11-09 | Celltech R&D Ltd | Biological products |
| BRPI0809042B1 (pt) | 2007-03-22 | 2021-08-31 | Biogen Ma Inc. | Proteína de ligação a cd154 isolada, seu uso, e composição |
| EP2535350B1 (en) | 2007-09-26 | 2018-01-24 | UCB Biopharma SPRL | Dual specificity antibody fusions |
| US7662587B1 (en) | 2009-03-05 | 2010-02-16 | E. I. Du Pont De Nemours And Company | Gene knockout mutations that increase peptide production |
| HUE040306T2 (hu) | 2009-09-24 | 2019-03-28 | Ucb Biopharma Sprl | Baktériumtörzs rekombináns fehérje expresszálására, amely tartalmaz proteázhiányos, de chaperonaktivitását megtartott DEGP-t és génkiütött TSP és PTR gént |
| US8470552B2 (en) | 2009-10-12 | 2013-06-25 | Keck Graduate Institute | Strategy to reduce lactic acid production and control PH in animal cell culture |
| CA2780143A1 (en) | 2009-11-05 | 2011-05-12 | Genentech, Inc. | Methods and composition for secretion of heterologous polypeptides |
| GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000591D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000588D0 (en) * | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201001791D0 (en) | 2010-02-03 | 2010-03-24 | Ucb Pharma Sa | Process for obtaining antibodies |
| GB201012599D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Process for purifying proteins |
| EP2546267A1 (en) | 2011-07-13 | 2013-01-16 | UCB Pharma S.A. | Bacterial host strain expressing recombinant DsbC |
| EA031449B1 (ru) | 2011-07-13 | 2019-01-31 | Юсб Фарма, С.А. | Бактериальный штамм-акцептор, экспрессирующий рекомбинантный dsbc |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
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