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SA522440743B1 - Paps طريقة لإنتاج بولي ساكاريد كبريتي وطريقة لإنتاج - Google Patents

Paps طريقة لإنتاج بولي ساكاريد كبريتي وطريقة لإنتاج

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Publication number
SA522440743B1
SA522440743B1 SA522440743A SA522440743A SA522440743B1 SA 522440743 B1 SA522440743 B1 SA 522440743B1 SA 522440743 A SA522440743 A SA 522440743A SA 522440743 A SA522440743 A SA 522440743A SA 522440743 B1 SA522440743 B1 SA 522440743B1
Authority
SA
Saudi Arabia
Prior art keywords
producing
paps
present
transformant
raw materials
Prior art date
Application number
SA522440743A
Other languages
English (en)
Inventor
نيشيبارا اكي
كاتو رايوسوكي
هاشيموتو شين-أيشي
كينتا فوكوناجا
كوراتسو ماساهيرو
هاياشي ميكيرو
Original Assignee
اوتسوكا فارماسيوتيكال فاكتوري، انك.
رينسلير بوليتيكنيك انستيتيوت
كيرين بايوماتيريالز كو.، ليمتد
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by اوتسوكا فارماسيوتيكال فاكتوري، انك., رينسلير بوليتيكنيك انستيتيوت, كيرين بايوماتيريالز كو.، ليمتد filed Critical اوتسوكا فارماسيوتيكال فاكتوري، انك.
Publication of SA522440743B1 publication Critical patent/SA522440743B1/ar

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01025—Adenylyl-sulfate kinase (2.7.1.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07004—Sulfate adenylyltransferase (2.7.7.4)
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    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
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    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03017—Heparosan-N-sulfate-glucuronate 5-epimerase (5.1.3.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

يتعلق الاختراع الحالي بتوفير طريقة للإنتاج السريع للبولي ساكاريدات المكبرتة عن طريق تفاعل نظام إنتاج/ إعادة توليد PAPS باستخدام النشاط الأيضي لكائن حي دقيق أو مادة معالجة منها باستخدام كائن حي دقيق يعبر عن إنزيم كبرتة أو مادة معالجة أو مستخلص منه أثناء خلط المواد الخام غير المكلفة مثل كبريتات ماغنسيوم. يتمثل هدف آخر للاختراع الحالي في توفير طريقة عملية لإنتاج PAPS من المواد الخام غير المكلفة. يتعلق الاختراع الحالي بطريقة لإنتاج بولي ساكاريد كبريتي وطريقة لإنتاج PAPS، تشتمل الطرق على خطوة تحضير عامل تحويل (أ) لبكتريا من الجنس الوتدية، حيث يشتمل على الأقل على جين يشفر ATP سلفوريلاز وجين يشفر APS كيناز التي يتم إدخالها بها بطريقة يمكن التعبير عنها، وحيث بها غشاء البلازما الخلوي بعامل التحويل (أ) يكون قابل لإنفاذ المادة، أو المادة المعالجة بعامل التحويل (أ)؛ وخطوة تنفيذ تفاعل لإنتاج PAPS باستخدام محلول تفاعل يحتوي على ATP أو مصدر ATP، مصدر أيون كبريتات، وعامل التحويل (أ) أو المادة المعالجة منه. شكل 1.
SA522440743A 2020-04-03 2022-09-29 Paps طريقة لإنتاج بولي ساكاريد كبريتي وطريقة لإنتاج SA522440743B1 (ar)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2020/015388 WO2021199445A1 (en) 2020-04-03 2020-04-03 Method for producing sulfated polysaccharide and method for producing paps

Publications (1)

Publication Number Publication Date
SA522440743B1 true SA522440743B1 (ar) 2024-05-30

Family

ID=77928740

Family Applications (1)

Application Number Title Priority Date Filing Date
SA522440743A SA522440743B1 (ar) 2020-04-03 2022-09-29 Paps طريقة لإنتاج بولي ساكاريد كبريتي وطريقة لإنتاج

Country Status (11)

Country Link
US (1) US12264350B2 (ar)
EP (1) EP4127204A4 (ar)
JP (2) JP7398684B2 (ar)
KR (1) KR20230004466A (ar)
CN (1) CN115605603A (ar)
AU (1) AU2021249958B2 (ar)
BR (1) BR112022019828A2 (ar)
CA (1) CA3174029A1 (ar)
IL (1) IL296974A (ar)
SA (1) SA522440743B1 (ar)
WO (2) WO2021199445A1 (ar)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119752761B (zh) * 2025-03-07 2025-06-17 北京化工大学 一种高产heparosan的基因工程菌及其应用

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JPS5830464B2 (ja) 1980-04-07 1983-06-29 株式会社 河原 作業台
JP3098591B2 (ja) 1991-11-14 2000-10-16 ユニチカ株式会社 ３’−ホスホアデノシン−５’−ホスホ硫酸の製造方法
US5919673A (en) * 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
JPH11187883A (ja) * 1997-12-26 1999-07-13 Human Science Shinko Zaidan ヒト由来ａｐｓキナーゼ／ａｔｐスルフリラーゼ 遺伝子
WO2000029603A2 (en) * 1998-11-18 2000-05-25 Neose Technologies, Inc. Low cost manufacture of oligosaccharides
JP4505011B2 (ja) 2005-01-25 2010-07-14 ヤマサ醤油株式会社 ３’−ホスホアデノシン−５’−ホスホ硫酸の酵素合成法
IN2014DN02837A (ar) * 2005-05-12 2015-07-10 Univ North Carolina
CN102712942B (zh) 2009-09-01 2014-05-14 伦斯勒理工学院 K5肝素前体的发酵和纯化
KR20110035805A (ko) 2009-09-30 2011-04-06 씨제이제일제당 (주) 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법
JP5730328B2 (ja) 2010-10-29 2015-06-10 ヤマサ醤油株式会社 育毛剤組成物
JP5455248B2 (ja) 2011-03-28 2014-03-26 ヤマサ醤油株式会社 保湿作用を有する皮膚外用剤
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Publication number Publication date
AU2021249958B2 (en) 2024-08-29
IL296974A (en) 2022-12-01
JP2023515250A (ja) 2023-04-12
TW202204632A (zh) 2022-02-01
WO2021201282A1 (en) 2021-10-07
JP7398684B2 (ja) 2023-12-15
US20230159969A1 (en) 2023-05-25
AU2021249958A1 (en) 2022-09-22
US12264350B2 (en) 2025-04-01
JP2023171870A (ja) 2023-12-05
JP7679041B2 (ja) 2025-05-19
KR20230004466A (ko) 2023-01-06
WO2021199445A1 (en) 2021-10-07
EP4127204A4 (en) 2024-06-26
CN115605603A (zh) 2023-01-13
BR112022019828A2 (pt) 2023-02-23
EP4127204A1 (en) 2023-02-08
CA3174029A1 (en) 2021-10-07

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