RU2335925C2 - Biologically active preparation and method of its manufacturing - Google Patents

Abstract

FIELD: food products.

SUBSTANCE: alfalfa-based biologically active preparation (BAP) is manufactured by treatment with extraction agent and contains macro-and micronutrients, amino acids, protein, lipids, amino sugars, organic acids, carbohydrates and vitamins in the specified ratio. Method of BAP manufacturing involves grinding plant raw material, its treating with extraction agent which contains water solution of macro-and micronutrient salts, holding the extraction mixture under increased temperature and pressure with further separation, refinement and drying of the extract. Primarily plant raw material is extracted by condensate heated up to 60-80°C with mass relation of the plant raw material to the condensate equal to 1:6. Afterwards part of the extract is put into intermediate tank. The remaining part is supplied to the "hot extraction" apparatus warmed by water steam, and water solutions of macro- and micronutrients are introduced during mixing. The relation of the extract water solution to plant raw material accounts for 6:1 wt. Hot extraction is carried out under the temperature of 110-140°C and pressure 1.5 - 4.5 atm during 0.6 - 1.2 hours. Then excessive pressure is released and temperature is lowered down to 100°C. The mixture is further held for 0.2 - 0.8 hours. Then the extract is supplied for filtration and evaporation to the concentration of 45-50%. The evaporated extract is mixed with cold extract with mass relation 4.0-6.0 to 36.0. Finished mixture is bottled or dried and then packed.

EFFECT: invention allows manufacturing of a product with increased biological activity.

3 cl, 1 dwg, 13 tbl

Description

The invention relates to the field of biotechnology, and specifically to the production of biologically active agents with immunomodulating properties, based on plant materials, and may find application as a food additive for therapeutic and preventive purposes, as well as in the cosmetic, pharmaceutical industry, in veterinary medicine, as well as as a feed additive for animals and birds.

According to the order No. 117 of the Ministry of Health of the Russian Federation dated 04.15.97 "On the procedure for examination and hygienic certification of biologically active food additives": "biologically active food additives are concentrates of natural or identical to natural biologically active substances intended for direct intake or administration in the composition of food products in order to enrich the human diet with individual biological substances and their complexes. ”

It is known that for normal life, to maintain one’s health, a person needs proteins, carbohydrates, fiber, fats (the required amount), vitamins, macro- and micronutrients, ultramicroelements. The introduction into the human body of any element in doses exceeding permissible is poison.

Vitamins and minerals are involved in the regulation of most vital processes and biochemical reactions in our body. An important role in providing the body with a number of vitamins (K, B ₁₂ , H) belongs to the microflora of the digestive tract. Only a few vitamins, such as A, D, E, B ₁₂ , can accumulate in the body. Therefore, vitamin deficiency entails vitamin deficiency diseases.

Deficiency of vitamins and minerals can cause severe inflammatory and hormonal disorders.

So, for example, for a person, the daily need for some macro- and microelements is (in mg - data are taken on average for a weight of 65 kg):

Macronutrients (daily requirement of more than 100 mg)

Na - 1100-3300

K - 1900-5600

Sa - 800

Mg - 350

Cl - 1700-5100

P - 800

S - 200

Trace elements (daily requirement less than 100 mg)

Fe - 10

Zn - 15

Mn - 2-5

Cu - 2-3

Cr - 0.05-0.2

Mo - 0.15-0.5

Se - 0.05-0.2

I - 0.15

F - 0.0015-0.004

Vitamins:

I. Fat-soluble (vitamins A, D, E, K) in mg:

Vitamin A (Retinol) - 1.0

With a lack of vitamin A, night blindness develops, growth disturbance is observed;

Vitamin D (Calciferol) - 0.01

Deficiency in the body of vitamin D leads to a violation of the mineralization of bone tissue;

Vitamin E (tocopherol) - 10

It is an effective antioxidant;

Vitamin K (phylloquinone) - 0.08

Vitamin K is involved in the carboxylation of glutamic acid residues in plasma proteins, which is important for normalizing or accelerating the blood coagulation process.

II. Water-soluble vitamins of the 1st and 2nd groups:

1st group (in mg)

Vitamin B ₁ (thiamine) - 1.5

With a lack of vitamin B ₁ there is a violation of the nervous system, as well as cardiovascular disease;

Vitamin B ₂ (a complex of vitamins including riboflavin, folic, nicotinic, pantothenic acid)

riboflavin - 1.8;

folic acid - 0.2;

nicotinic acid - 20;

pantothenic acid (vitamin B ₃ ) - 7.

Riboflavin acts as a carrier of hydrogen, folic acid deficiency leads to anemia, nicotinic acid deficiency leads to indigestion and depression, vitamin B ₃ deficiency is rare, since pantothenic acid is part of many products.

2nd group (in mg):

Vitamin B ₆ (pyrodoxal, pyridoxine, pyridoxamine) - 2.0

Participates in the metabolism in the body, activates amino acids;

Vitamin B ₁₂ (cobalamins - cyanocobalamin) - 0.002

Vitamin B ₁₂ deficiency is mainly associated with the cessation of secretion of the internal factor, resulting in pernicious anemia;

Vitamin C (L-ascorbic acid) - 60.0

It is a stabilizer of enzymatic systems, coenzyme, antioxidant. A consequence of vitamin C deficiency is a disorder of the hematopoiesis system, tooth loss.

Vitamin H (Biotin) - 0.1

Participates in metabolism, is a carrier of carboxy groups [J. Kolman, K.-G.Rem. Visual biochemistry. M., "World", 2000, S. 350-357].

Various pathological disorders in the human and animal body cause deviations from the norm of the parameters of the physiological state. In these cases, the therapeutic effect on the body, including the preventive effect, consists in stabilization, that is, maintaining these parameters in the norm or (if they deviate from the norm) in such an effect on the body in which these parameters normalize.

Normalization of the physiological state of the human and animal organisms can be carried out in two directions: firstly, by directly affecting the pathology and, secondly, by indirectly affecting the systems, organs and tissues of the body, which are responsible for maintaining normal parameters of homeostasis.

The introduction of hormonal drugs, mediators, growth factors, chemotherapy, and the like, provide a direct effect on target cells through reception. For example, in the treatment of diabetes, insulin is administered to supplement the functional deficiency of the pancreas; in case of impaired functional activity of the nervous system, neurotransmitters are introduced that contribute to the normalization of the threshold sensitivity of neurons and, accordingly, to the improvement of transmission of nerve impulses; in cases of impaired functional activity of the body’s immune system, immuno-mediators are used that correlate the immune response, and so on.

Statistics confirm a massive and persistent deficiency in our food of vital proteins, vitamins, and trace elements. There are no reserves of these substances in the body, they cannot be put off for the future, therefore they must be contained in the daily diet. Most modern refined food products are deprived of these micronutrients, therefore, new types of food products for general and special purposes using dietary supplements are needed.

To solve many problems associated with human life, scientists suggest introducing biologically active substances from natural sources into the human body, both in the form of separate concentrates and in the form of food products that contain macro- and microelements, vitamins, polyunsaturated fatty acids, essential amino acids, some mono- and disaccharides, dietary fiber, etc.

A biologically active agent with an immunomodulating effect is known for use in medicine and veterinary medicine and a method for its preparation according to patent RU No. 2041717, IPC A61K 35/55, 1992, in particular, to restore the functional activity of organs and tissues - to normalize the physiological state organism. The biologically active agent in accordance with the invention relates to substances of animal origin and includes organic compounds such as peptides, amino acids, carbohydrates, lipids and other organic substances.

A disadvantage of the known biologically active agent is the high content of high molecular weight compounds in it, which are immunogens, leading to the appearance of side reactions, such as allergies, hypersensitivity, and also the method for producing this agent is not very productive and very laborious due to the use of feedstock of animal origin.

A biologically active agent in the form of a powder is known, containing: lipids 0.13-0.25 wt.%, Amino acids and peptides of at least 0.80 mg / g, cholesterol 0.10-0.20 mg / g, phosphorus 3.5-9.7 wt.%, Calcium 0.15-20.0 wt.%, Iodine 0.02-0.04 wt.%, Magnesium less than 6800 μg / g, barium less than 31.30 μg / g , manganese less than 3.5 μg / g, tin 0.50 μg / g, copper 0.5-20 μg / g, iron 60-240 μg / g, zinc 60-120 μg / g, lead less than 0.35 μg / g, cadmium less than 0.001 μg / g, cesium 4-12 μg / g, strontium 5-10 μg / g, potassium 300-600 μg / g [RU No. 2077887, IPC A61K 35/32, 1996]. This biologically active substance of animal origin is made of ossified deer antlers.

The disadvantages of the above funds should be attributed to the fact that it has a pronounced side effect due to the significant content of proteins and peptides, many of which are toxic, these drugs have hemolytic and allergenic properties. The high cholesterol content stored in the finished product causes atherosclerosis, lipoid hyperplasia and other diseases associated with excess intake of cholesterol. The method of preparation of this tool is associated with a shortage of raw materials, the difficulty of its multi-stage processing. To a large extent, the technology of preserving the latter, the method of extraction, affects the activity of extracts from antlers. In the process of both preserving and extracting, a noticeable destruction of valuable components of the initial product of proteins, peptides, and blood elements occurs. Without canning, antlers can only be stored for 6-10 hours after cutting or up to 10 days at a temperature of -4 ° C.

In addition, the generally accepted methods for the extraction of antlers do not ensure the complete isolation of pharmacologically valuable substances, in other words, all known preparations from deer antlers are to a certain extent poor in composition in comparison with the initial product. More than 90% of the total content of valuable and biologically active components of antlers remains in the waste product in the cake.

It is also known biologically active substance used as a biologically active food supplement based on pectin-containing plant materials, which is a plant pectin-cellulose complex, additionally contains an organic matrix hydrolyzate and mineral substances obtained from bone tissue in the following ratio, wt.%: Calcium 2 8-3.0; phosphorus 1.6-1.8; magnesium 0.20-0.25; potassium 0.015-0.025; iron 0.04-0.06; mixtures of amino acids 0.5-0.7; proteins and oligopeptides 8.0-9.2; pectin-cellulose complex 42-45; lignin 7.0-9.0; fiber 22.5-24.51; sucrose no more than 0.1; moisture no more than 14; heavy metals not more than 0.002.

In the method for producing dietary supplements for food, including treating pectin-containing plant materials with saturated lime water, washing with water, hydrochloric acid and deionized water in series, maintaining the resulting extract in a solution of mineral salt, washing and drying, the bone tissue is further processed by acid decalcification, neutralization of the obtained solution, washing the mineral precipitate, mixing it with an acid hydrolyzate of a decalcified organic matrix to obtain an amine oacyl salts of minerals, followed by drying; after which the products of processing bone tissue and plant material are mixed together in a ratio of 1:10 [RU No. 2213492, IPC A23L 1/30, 2001].

This biologically active substance does not contain many vital trace elements, which does not sufficiently ensure the regulation of metabolism and the functions of immunocompetent organs.

The biologically active substance "MOS Lenkin" is known [RU No. 2111680, IPC A23L 1/30, 1996], which is obtained from pre-crushed mountain ore from one geological mining region containing mummy-like substances, characterized by the following features: a biologically active aqueous extract of pre-crushed mountain ore containing mummy-like substances, which is extracted with water, the extract is decanted, filtered, purified from heavy elements, concentrated and / or dried, and a substance is obtained with the following chemical composition, wt.%:

Mono-, di-, tripeptides - 25.0-30.0,

including conjugated amino acids, mg amino acids per 100 g of concentrate:

Aspartic Acid - 200-500

Threonine - 70-150

Series - 80-140

Glutamic acid - 250-4700

Glycine - 2500-4300

Alanine - 350-1400

Cystine - 100-120

Isoleucine - 30-100

Leucine - 50-140

Histidine - 150-270

Lysine - 80-110

Arginine - 150-570

Valin - 30-40

Methionine - 10-18

Phenylalanine - 40-95

Tyrosine - 30-50

Proline - 1200-1500

γ-aminobutyric acid - up to 1.0,

including free amino acids, mg amino acids per 100 g of concentrate:

Aspartic acid - 10-100

Oxyproline - 70-75

Glutamic acid - 4.5-5.0

Glycine - 1000-4000

Alanine - 20-30

Valin - 10-20

Isoleucine - 2-3

Leucine - 5-6

Tyrosine - 7-8

α-alanine - 10-11

Phenylalanine - 4-5

γ-aminobutyric acid - up to 1.0

Histidine - 2-3

Arginine - up to 1.0

Minerals - 30.0-50.0,

including micro-, macrocells, mcg per 100 g of concentrate:

Aluminum - 3.0-43.0

Boron - 12.0-190.0

Barium - 10.0-42.0

Beryllium - 0.02-0.20

Calcium - 1600.0-18000.0

Cadmium - 0.1-1.0

Cobalt - 2.0-3.0

Chrome - no more than 0,001

Copper - 1.0-15.0

Iron - 12.0-240.0

Mercury - Traces

Potassium - 1000.0-6300.0

Lithium - 0.3-3.0

Magnesium - 1300.0-22400.0

Manganese - 53.0-58.3

Molybdenum - 0.02-2.0

Sodium - 2400.0-73000.0

Nickel - 0.1-5.0

Phosphorus - 20.0-193.0

Tin - Traces

Antimony - Traces

Selenium - Traces

Silicon - 13.0-145.0

Lead - 0.1-0.8

Strontium - 20.0-162.0

Titanium - 0.2-2.5

Sulfur - 10.0-1000.0

Reducing substances - 2.0-4.0

Organic acids - 2.0-3.0

Volatile and non-volatile aromatic substances - not more than 4.0

Brown pigments, polyphenols, melanoidins - not more than 10.0

The well-known biologically active substance has a bioprotective property, maintains a state of high physical and mental performance in extreme environmental conditions;

- increases the stability of poi hypo- and hyperthermia, hypobaria, improves the hematopoiesis system, has anti-inflammatory, regenerative properties;

- shows an increase in the immune-protective properties of debilitated people, patients with extensive severe burns, with purulent-septic conditions, patients in the intensive care unit after surgery, patients with drug addiction and chronic alcoholism;

- shows an increase in the protective properties of the body from thermal, bacteriological, chemical, radiation and other lesions.

On the basis of the claimed substance "MOS Lenkin", promising developments of a new class of food compositions of bioprotective action are possible, as well as the preparation of new technical solutions in biotechnology, medicine, animal husbandry, and crop production. The positive radioprotective effect of "MOS Lenkin" is of great practical importance in radiotherapy, rehabilitation of patients in the postradiation period [RU No. 2111680, IPC A23L 1/30, 1996].

Although this biologically active substance can be called a "panacea", a universal tool, but it is characterized by high cost due to the use of selected mummy-containing rocks and the complexity of obtaining the tool itself.

As the analysis of the prior art has shown, recently the direction of processing natural raw materials to produce biologically active complexes has been very actively developing. Peptide-containing animal raw materials (waste from the processing of animal raw materials, animal blood), mountain ore containing mummy-like substances, microorganisms, vegetables, etc. are used as natural raw materials.

The prior art biologically active composition having the property of a biological stimulant, which is a protein hydrolyzate obtained by grinding the feedstock (protein-containing waste products of livestock production), acid hydrolysis of the feedstock in 5-20% sulfuric acid for 5-15 hours at a temperature of 90-110 ° C, subsequent filtration of the acid hydrolyzate, then the alkaline hydrolysis of the residue, which is not dissolved in the acid, is carried out with calcium oxide at 95-105 ° C for 3-5 hours, mixing and stirring m of acid and alkaline hydrolysates, pH correction (if necessary), filtration and freeze-drying of the final solution (MOS), while it has the following composition, wt.%: amino acids 65-67, including free amino acids: aspartic 7.32, threonine 0.69, serine 1.38, glutamine 3.88, valine 1.73, proline 5.22, methionine 0.89, alanine 2.39, leucine 1.13, isoleucine 0.56, histidine 22.16, phenylalanine 0.61, arginine 1.56, lysine 1.28, fats 18-20, lipids other than fats, 2-2.5, carbohydrates 8-11, organic and inorganic salts 0.5-2, macronutrients 0, 2-2, including mg / g: calcium 5.72, potassium 7.1 3, sulfur 24.09, phosphorus 19.15, chlorine 2.0, trace elements 0.0005-0.009, including μg / g: iron 67.79, zinc 33.22, copper 8.50, manganese 5, 35 [RU No.2016510, IPC A01H 4/00, 1992].

A biologically active food supplement is also known, which can be obtained on the basis of the obtained biologically active substance and a component selected from the series: carbohydrates, lipids, vitamins, vitamin-like substances, mineral components, alkaloids, homeopathic remedies, glycosides, immunoglobulin preparations, lactoferrin, phenolic compounds, organic acids, enzymes, terpenoids, hormones and hormone-like substances, cytokines, plant products, microbial products, bee products water, animal products, mineral substances of natural origin, detoxicants, processing aids or mixtures thereof, in the following ratio of components, wt.%: BAS - 10-90, component or mixture of components - the rest [RU No. 2221456, IPC A23L 1/30 , 1/16, 1/24, 1/2003]. In this case, carbohydrates are selected from the series: glucose, fructose, lactose, starch, sucrose, maltose, deoxyribose, soluble and insoluble dietary fiber, inulin, ribose.

Lipids are selected from the series: triglycerides, phospholipids, sterols, sterols, microbial lipids.

Vitamins are selected from the series: Vitamin A, Vitamin D, Vitamin E, Vitamin K, Vitamin B1, Vitamin B2, Niacin, Vitamin PP, Nicotinic Acid, Pantothenic Acid, Pyridoxine, Folic Acid, Folacin, Cyanocobalamin, Vitamin C, and Vitamin-Like Substances from the series: biotin, bioflavonoids, orotic acid, pangamic acid, para-aminobenzoic acid PABA, choline, inositol, vitamin T, carnitine, vitamin U, lipoic acid, α-lipoic acid, thioctic acid.

Mineral components are selected from the series: calcium, phosphorus, magnesium, sodium, potassium, sulfur, chlorine, iron, copper, selenium, iodine, chromium, zinc, fluorine, manganese, cobalt, molybdenum, silicon, bromine, silver, boron, vanadium, germanium.

Alkaloids are selected from the series: pyrrolidine derivatives, piperidine derivatives, pyridine derivatives, quinoline derivatives, isoquinoline derivatives, indole derivatives, imidazole derivatives, purine derivatives, diterpeno alkaloids, steroid alkaloids, glycoalkaloids, acyclic and peptide derivatives.

Glycosides are selected from the series: saponins, antraglycosides, bitter glycosides, thioglycosides.

Phenolic compounds are selected from the series: simple phenols, phenolospiets, phenolic acids, coumarins, chromones, lignans, flavonoids, isoflavonoids, flavonones, flavones, rotenoids, tannins, lignin, melanins, humic acids.

The disadvantages of the above additives include their high cost associated with the processing of raw animal feed and the difficulty of obtaining the biologically active substance itself. In addition, the method of manufacturing a dietary supplement excludes the possibility of long-term preservation of vitamins and such valuable bioactive additives as flavones, flavonoloids, flavonoids.

Known biologically active composition for producing biologically active feed additives from natural raw materials for farm animals that contain vitamins, macro- and microelements and other biologically active substances (BAS). The proposed method is as follows: prepared natural raw materials: vegetable, and / or fruit, and / or berry, and / or grain, and / or melon, and / or legume, and / or algae, and / or grass, including and medicinal, and / or leaves, and / or branches, and / or roots, and / or roots, and / or shell of eggs, and / or shells of mollusks, and / or shells, and / or horns of wild animals, for example reindeer pre-ground to a particle size of 10-35 mm, and then it was dried at a pressure 0,01-1,033 kg / cm ² and 10-150 ° C to a moisture content of not more than 10%, then dried mass COOLING they are heated to a temperature of -30 to -180 ° C over the entire volume, for example, in liquid nitrogen, after which the cooled raw material is crushed to a particle size of 1.0-1000 μm, followed by packaging of the resulting product, and the product can be transferred before packaging in dosage form, for example in granules.

This biologically active composition contains protein, vitamins (carotene, vitamins E, C, carbohydrates, monosaccharides, polysaccharides [RU No. 2232514, IPC A23K 1/16, 2002].

The proposed method expands the arsenal of methods for producing dietary supplements for animal feed, is intended for use in agriculture and allows you to get biologically active additives (BAA) to feed from natural raw materials in a wide range, including both plant materials and animal feed, additives retain up to 80%, and in some cases up to 100% of biologically active substances contained in the feedstock, while they retain the organoleptic properties of the feedstock, have a large active surface with increased p the permeability of cell membranes and, as a consequence, high bioavailability and sorption ability, so they can be successfully used as dietary supplements for the main feed.

Currently, biologically active compounds have been developed both in Russia and abroad for the production of biologically active food additives based on plant materials.

Known dietary supplements containing soybean phospholipids - "Stem Lecithin" (Germany), "Granular lecithin" and "Moslecithin" (Russia), in which phosphatidylcholine accounts for 23%; "Lecithincholine" containing 35% phosphatidylcholine (Emrion, USA); "Premium lecithin" of the company "New Spirit Naturals" and others [Semenova S.B. Health supplements in food. Directory. M., "Deca", 1998, p.256]. Recommended consumption doses range in a fairly wide range (from 1 to 14 g per day, and in some cases up to 35 g).

Supplements containing phospholipids as an ordinary component are also known, for example, the Beauty Supplement (Vision International People Group), which is a vitamin complex with plant and animal components, in which lecithin (soybean phospholipid complex) accounts for 6%. Or, for example, dietary supplement Take Hat (Emrion, USA), in which phospholipids account for 11%, and which also contains choline, inositol, lipoic acid, vitamins, minerals and chondroitin sulfate.

Known food supplement containing phosphatidylcholine (PF), phosphatidylinositol (PI) and / or phosphatidylethanolamine (PE), soya lecithin, one or more mucopolysaccharides, niacin and silicon dioxide, silicic acid and / or organic silicic acid esters [US Pat. US 4466958, 1984]. Supplements are intended to improve the condition of patients with cardiovascular disorders in the recommended dose of 4-32 g daily.

Compositions are known that provide essential omega-3 and omega-6 fatty acids and contain phospholipids rich in polyunsaturated fatty acids with a long carbon chain, isolated from the yolk of eggs from hens contained on a special diet, soya lecithin, one or more mucopolysaccharides that are in different options may contain vitamins E, C and niacin, as well as casein and acceptable carriers [Pat. US No. 5669530, 1999]. The compositions may be presented in the form of a powder added to milk for infants, in the form of a granulate added to the food of children and the elderly.

It is known that phospholipids are the main structural component of all cell and subcellular membranes, ensuring their normal structure and thereby the normal functioning of cells [Canty D.J., Zeisel S.H / Nutrition Reviews 52 (10), 327-339]. The similarity of plant phospholipids with human intrinsic phospholipids and the ability of soybean phospholipids to integrate into cell biomembranes and blood lipoproteins due to this similarity allows plant, in particular soybean, phospholipids to prevent a number of possible pathological processes. Experimental and clinical studies have shown that phospholipids play an important role in the metabolism of the human body, exhibit membrane-stabilizing, hepatoprotective, lipotropic, anti-atherogenic and a number of other properties. Without antioxidant properties, phospholipids are synergists of many antioxidants that are part of the non-enzymatic antioxidant defense system of lipids in the human body, for example tocopherols [Burlakova EB and other Biological membranes, RAS, 1998, 15 (2), p.137-166]. In addition, they contribute to the absorption and better absorption of fat-soluble vitamins A, D, E and K; ubiquinone and a number of other bioactive substances.

As dry extracts of medicinal plants, the composition contains one or more extracts listed below:

- roots of Scutellaria baicalensis (Scutellaria baicalensis Georgi)

- Hypericum perforatum L. (Hypericum perforatum L.)

- rhizomes of the safflower levzea (Rhaponticum carthamoides Willd). (Ilijn)

- green leaves and young shoots of tea bush L. (Camellia sinensis)

- rose hips (Rosa L.)

- roots and herbs of dandelion officinalis (Taraxacum officinale wigg)

- prickly roots of Eleutherococcus (Eleutherococcus senticosus Rupr. et Maxim)

- roots and herbs of Echinacea purpurea (Echinacea purpurea L.)

- fruit hops common (Humulus lupulus L.)

- herbs of oregano (Origanum vulgare L.)

- rhizomes and roots of Rhodiola rosea (Rhodiola rosea L.)

- fruits of Schisandra chinensis (Schizandra chinensis Baill.)

- fruits of milk thistle (Silybum marianum L.)

- inflorescences of hibiscus or hibiscus (Hibiscus sabdariffa)

- flavonoid complex of Siberian larch wood (Larix sibirica Ledeb.)

- chokeberry leaves (Aronia melanocarpa (Elliot.) Michx.)

- the aerial parts of the multiconductor hairworm (Bupleurum multinerve DC.)

- rhizomes of the cinquefoil erect or Kalgan (Potentilla erecta L.)

- flower baskets of calendula officinalis (Calendula officinalis L.)

- leaves of frangipani (Bergenia crassifolia (L.) Fritsch)

- leaves and buds of a three-leaved watch (Menyanthes trifoliata L.)

- leaves of a large plantain (Plantago major L.)

- peppermint leaves (Mentha piperita L.)

- Thallus of Fucus vesiculosus (Fucus vesiculosus L.)

A dietary supplement based on the indicated composition may additionally include coenzyme Q10 and / or individual vitamins or vitamin premixes and / or trace elements zinc and / or selenium and pharmaceutically acceptable excipients (microcrystalline cellulose, starch, lactose, magnesium stearate, calcium stearate, calcium carbonate, etc.) in an amount of 15-22 wt.% [RU No. 2252529, IPC A61K 35/78, 2003].

Although the known additive contains a sufficient amount of useful elements, but still in these compositions there is an insufficient amount of trace elements such as cobalt, vanadium, and molybdenum.

A well-known biologically active food supplement is a tablet and contains a germanium-organic compound - 2-carboxyethyl-hermesesquioxane (KGS), fat-soluble vitamins A and E, water-soluble vitamins C and B ₆ , skimmed milk powder, minerals potassium and phosphorus in the form of potassium dihydroortho fructose, calcium stearic acid and polyvinylpyrrolidone in a certain ratio. The invention allows to obtain a product with increased bioantioxidant activity and improved digestibility [RU No. 2262276, IPC A61L 1/30, 2003].

The disadvantages of this biologically active composition include the difficulty of its preparation and the deficiency of germanium-containing compounds.

The search for new non-traditional sources for dietary supplements is an urgent task. A particularly urgent task is the search for natural (natural) sources of source material, for example plant materials.

Known biologically active composition based on the mother liquor of sodium humate. The additive is characterized by the following composition in mg / l of the mother liquor of sodium humate: total protein - 800.5; total lipids - 5004; potassium - 12.0; sodium - 500.0; calcium - 59.5; iron - 12.5; copper - 0.08; zinc - 0.19; cobalt - 0.02; cadmium - 0.008; manganese - 0.43; ammonium nitrogen - 20.7; nitrates - 0.02; total phosphorus - 16.0; vitamin C - 16.75; triglycerides - 100.59; amylase - 1200-1300; sodium humate - 4800; humic acids - 3600; iodine - 0.12; lysine - 20.0; histidine - 10.0; aspartic acid - 10.0; threonine - 10.0; series - 5.0; glutamic acid - 20.0; proline - 10.0; leucine - 10.0; glycine - 2.0; valine - 3.0; methionine - 0.1; isoleucine - 4.0; tyrosine - 10.0; phenylalanine - 20.0; Alanine - 2.0.

The invention allows to obtain a biologically active additive, which is able to eliminate the deficiency of micro and macro elements, vitamins and amino acids in the human body, restore normal metabolism, and also cleanse the body of toxins, allergens and radionuclides [RU No. 2259147, IPC A61K 1/30, 2003] .

Although the above composition contains a sufficient amount of useful substances, but at the same time it also has drawbacks, namely, an insufficient number of necessary trace elements.

Also known is a biologically active additive based on alfalfa hay and the method for producing it according to patent RU No. 2176918, IPC A61K 35/78, 2000. This additive is produced under the brand name "Luceutovite", has a registration number 004836.P.643.09.2002, manufactured in accordance with TU 9749-001-04269058-02, has the expert opinion of the State Research Center at the GUNII RAMS No. 72 / E1709 / I02.

Known additive obtained on the basis of alfalfa extract, in accordance with the above TU, contains in 1 gram of dry product:

saponins, flavones, isoflavones, sterols,

coumarin trees, a significant amount of proteins, alpha and beta carotenes, flavonoids - 2 mg;

vitamins of the group:

A - 0.03 mg

B1 -0.2 mg

B2 - 0.25 mg

B5 - 0.67 mg

B6 - 33.33 mg

E - 1.67 mg

C - 11.6 mg

K - 0.011 mg

hydro acids:

lemon - 0.012 mg

wine - 0.02 mg

apple - 0.005 mg

carbon (amber, oxalic) and

phenol carboxylic acids

proteins (amino acids): valine - 0.35 mg

leucine - 0.96 mg

methionine - 0.33 mg

Alanine - 2.5 mg, etc.

carbohydrates: glucose - 0,055 mg

fructose - 0.073 mg, etc.

macro- and microelements:

Fe - 0.354 mg

Zn - 0.133 mg

Cu - 0.0124 mg

Mn - 0.0325 mg

V - 0,033 mg

Mo - 0.0147 mg

Ti - 0.0147 mg

The opinions of the inventors according to patent RU No. 2176918 (Luzovit) are erroneous, for example, regarding the biochemical activity of titanium.

In the book of B.V. Nekrasov "Fundamentals of General Chemistry" - Ed. "Chemistry", M., 1974, v. 1, p. 646, p. 5. it is mentioned that the titanium compound has no biological value, although it is present in the mass of plants.

In the extensive monographs by Kolman J., Rem K.G. "Visual biochemistry" also does not say anything about titanium as an obligate element in biochemical processes.

The literary sources known to the authors also do not say anything about the human need for titanium.

The closest technical solution to the claimed one - the prototype - both in composition and in the method of production, is a biologically active drug based on alfalfa - "Dolyutsar" obtained by treating it with an extractant containing a mixture of salts of trace elements Ba, Sn, Co, Cr, V, Zn, Fe, Se, Mn, Cu in the following ratio, mg / kg of plant material:

VA - 13.0

Sn - 0.6

Co - 4.0

Cr - 1,0

V - 1.8

Zn - 700

Fe - 1030

Se - 1,0

Mn - 22

Cu - 1,0

[RU No. 2145863, IPC A61K 31 / 00.1998], which was produced according to TU 9199-002-12705616-01, was previously produced according to TU 494K-AO42-006-97 under the trademark Erakond.

This alfalfa extract contains amino acids, carbohydrates, flavonoids, physiologically important trace elements and other active biological compounds. The extract has a powerful immunomodulatory effect, strengthening specific and non-specific factors of the body's immunity. It restores the body's ability to self-regulate physiological functions. Due to this, the extract has a wide spectrum of action, in particular hepato- and hemoprotective, antiulcer, anti-inflammatory, wound healing, choleretic, stimulates reparative processes, etc. It is recommended, in particular, as a food additive in food products and perfumes and cosmetics for imparting therapeutic and prophylactic properties; as a remedy in medical nutrition; as a flavoring additive, the extract is added to bakery and confectionery products, dairy products, ice cream, sausages, soft drinks and alcoholic drinks, beer, etc. ["Dairy industry", No. 9, 2000, p.12].

From the data of Lukmanova K.A. [Development of multifunctional medicines and a biologically active additive based on an extract of alfalfa grass sowing - The dissertation for the degree of Doctor of Medical Sciences. Ufa, 2000], the drug "Herakond" has in its composition (except for macro- and microelements):

Table 1 Name of amino acid The amino acid content, mg / 100 g of the additive irreplaceable interchangeable min Max. min Max. Valine 112 168 Leucine 80 113 Isoleucine 42 63 Lysine 37 66 Methionine 21 45 Threonine 77 104 Phenylalanine 185 272 Alanine 202 297 Arginine 101 160 Glycine 158 194 Histidine 82 105 Tyrosine 92 126 Aspartic acid 269 363 Glutamine 593 745 Proline 102 130 Series 90 122 Cysteine 66 one hundred

table 2 Name of organic acid The content of organic acids, g / 100 g additives min Max. Ascorbic 0.2 0.56 Wine 0.04 0.34 Sorrel 0.05 0.45 Apple 0.23 0.75 Lemon 0.37 1.96

Table 3 Name of carbohydrates Carbohydrate content, g / 100 g additives min Max. Arabinose 0.30 0.73 Xylose 0.26 1.29 Fructose 4.08 10.62 Galactose 0.24 0.83 Glucose 2.67 8.33 Sucrose 0.45 1.49 Maltose traces 0.85

In addition to those indicated in tables 1-3, Dolucar (Heracond) contains:

The content of humic substances - 2.19-5.6 g / 100 g of product

The content of uronic acids is 3.0-10.0 g / 100 g of product

Flavonoid content - 2.1-2.8 g / 100 g of product

Protein - 15-20 g / 100 g of product

Ribose - 4.5-5.5 g / 100 g of product

The specific content of vitamins is not indicated, but it can be assumed that they are contained at the level of the drug "Lucevit".

As a prototype for the method of producing a biological additive, the authors took a method of producing a biologically active preparation based on alfalfa hay, including heat treatment of crushed raw materials with an extractant containing a solution of trace elements Ba, Sn, Co, Cr, V, Zn, Fe, Se, Mn, Cu in RF patent 2145863.

According to the prototype, a biologically active drug is prepared as follows.

Alfalfa crushed hay to a fraction of 3-5 mm is extracted with steam condensate containing microelements (the ratio of crushed plant material to the extractant is 1: 8), at 60-80 ° C, kept at a temperature of 150 ° C for 90 minutes and a pressure of 5.4 -5.9.10 ⁵ Pa. After a lapse of time, the obtained extract was filtered, evaporated to 40% concentration.

To obtain a dry target preparation, a 40% extract is fed into a spray dryer and dried at a temperature of 250-300 ° C.

The dried product is served on the packaging.

The disadvantages of the known drug and method include:

- relatively low biological activity;

- insufficient content of vitamins, which are destroyed due to extraction at elevated temperature and pressure;

- the duration and complexity of the technology;

- energy costs associated with the process at high temperatures and pressure;

- resinification of the target product, which affects its quality, loss of vitamins and other valuable components.

The technical task of the proposed complex invention is the development of an improved composition of a biologically active drug, increasing its biological activity, reducing costs in its production, reducing its cost and improving quality, as well as expanding the range of biologically active additives.

The problem is solved in that a biologically active drug based on alfalfa obtained by treating it with an extractant containing macro- and microelements, characterized in that it contains ingredients in the following ratio, mg / 100 grams:

Calcium - 2000-8000

Potassium - 4000-12000

Sodium - 4000-12000

Magnesium - 400-2000

Phosphorus - 1400-8000

Iron - 80-600

Zinc - 180-1000

Tin - 0.1-0.4

Cobalt - 0.1-0.4

Chrome - 1.0-2.0

Vanadium - 0.1-0.4

Manganese - 0.06-0.40

Molybdenum - 1.0-1.8

Copper - 6.0-20.0,

as well as

free amino acids, lipids, proteins, mg / 100 g of product:

Table 4 Name of amino acid The amino acid content, mg / 100 g of the additive irreplaceable interchangeable min Max. min Max. Valine thirty one hundred Leucine thirty 80 Isoleucine thirty 40 Lysine twenty 60 Methionine twenty one hundred Threonine twenty 80 Tryptophan twenty one hundred Phenylalanine twenty 120 Alanine thirty 130 Arginine twenty one hundred Glycine thirty 130 Histidine twenty one hundred Tyrosine twenty one hundred Asparagine twenty 60 Aspartic acid twenty 200 Glutamine twenty 60 Glutamine twenty 200 Proline twenty one hundred Serine twenty one hundred Cysteine thirty one hundred

Protein - 20000-30000 mg / 100 g of product,

Lipids - 100-1000 mg / 100 g of the product,

Aminosugar 2000-8000 mg / 100 g of product, as well as organic acids:

Table 4 ' Name of organic acid The content of organic acids, mg / 100 g of the additive min Max. Ascorbic 200 400 Wine one hundred 400 Sorrel fifty 400 Apple 300 800 Lemon 300 1500

carbohydrates:

Table 5 Name of carbohydrates The carbohydrate content, mg / 100 g of the additive min Max. Arabinose 300 800 Xylose 300 1500 Fructose 3000 8000 Galactose 300 1000 Glucose 3000 7000 Sucrose 400 1500 Maltose one hundred 600

vitamins:

Table 6 Name of vitamins The content of vitamins, mg / 100 g of product min Max. Vitamin A (Retinol) fifteen 80 Vitamin B1 (Thiamine) fifteen 80 Vitamin B2 (Riboflavin) twenty one hundred Vitamin B3 (PP, Niacin) 200 800 Vitamin B4 (Choline) twenty fifty Vitamin B5 (pantothenic acid) 70 600 Vitamin B6 (pyrodoxin) twenty 200 Vitamin B9 (Folic Acid) 10 80 Vitamin B12 (cyancobalamin) one fifteen Vitamin C (Ascorbic Acid) 60 120 Vitamin D (calciferol, calcitriol) one 10 Vitamin E (tocopherol isomers) 80 300 Vitamin K (phyllohydroquinone, vicasol) 10 80 Vitamin H (Biotin) 1,5 20,0 Vitamin P (bioflavonoids) 1,5 20,0

The problem is also solved by the fact that in the proposed method for the production of a biologically active preparation, including grinding vegetable raw materials, treating it with an extractant containing an aqueous solution of salts of macro- and microelements, holding the extracting mixture at elevated temperature and elevated pressure, with further separation, purification and drying the extract obtained, which is first carried out the extraction of plant materials with condensate, heated to a temperature of 60-80 ° C for 0.3-0.5 hours, while the mass ratio of races of raw material for condensate is 1: 6, then part of the extract is squeezed into an intermediate tank, and the rest is fed to “hot extraction” in a device heated by steam, and with stirring, aqueous solutions of macro- and microelements are introduced per 1 kg of plant material . Hot extraction is carried out at a temperature of 110-140 ° C and a pressure of 1.5-4.5 atm for 0.6-1.2 hours.

After that, the excess pressure is relieved, and the temperature is reduced to 100 ° C, and at this temperature the mixture can withstand another 0.2-0.8 hours. Next, the obtained extract is fed to filtration and then to evaporation, where evaporation takes place to a concentration of 45-50%.

To replenish the vitamins of groups A, B1-B12, C, D, E, K, P, H one stripped off the extract is combined with a cold extract, while the mass ratio of the cold extract to one stripped off is 4.0-6.0 to 36.0. The finished mixture is served for bottling or freeze-drying and then for packaging.

As an extractant for hot extraction, aqueous solutions of macro- and microelements Ca, K, Na, Fe, Zn, Mg, P, Sn, Co, Cr, V, Zn, Mn, Mo, Cu are used in the following ratio, mg / kg plant materials:

Calcium - 14.6-18.4

Potassium - 18.6-22.6

Sodium - 13.5-14.6

Magnesium - 1.4-2.0

Phosphorus - 21.0-22.5

Iron - 0.3-0.8

Zinc - 0.13-0.18

Tin - 0.21-0.31

Cobalt - 0.20-0.28

Chrome - 0.43-0.51

Vanadium - 0.04-0.09

Manganese - 0.06-0.40

Molybdenum - 0.02-0.06

Copper - 0.40-0.62

It should be noted that all vitamins are thermolabile substances and upon thermoextraction many of them are destroyed. And the combination of the "cold extract" with the "hot extract" allows you to save these vitamins, and not only vitamins, in the finished product, which generally increases the biological activity of the drug.

The invention is illustrated by the circuit diagram shown in the drawing.

The inventive method is implemented as follows.

First, for each batch of source hay alfalfa determine the content of micro and macro elements, the content of the total flavonoids.

Crushed hay alfalfa to a fraction of 3-5 mm is loaded into a heated apparatus with a stirrer 1, into which condensate I is preliminarily poured, heated to 60-80 ° C. Stirring is carried out for 0.3-0.5 hours. Next, part of the extract is transferred to the intermediate tank 5, and the rest is fed to the apparatus 2 for “hot extraction” heated by steam, and with stirring, aqueous solutions of macro- and microelements II are introduced based on 1 kg of plant material, while the ratio of the aqueous solution extract to plant material is 6: 1 wt. The mixture in the apparatus 2 is maintained at a temperature of 110-140 ° C and a pressure of 1.5-4.5 atm for 0.6-1.2 hours. After that, the excess pressure is relieved, and the temperature is reduced to 100 ° C, and at this temperature the mixture can withstand another 0.2-0.8 hours. Next, the obtained extract is fed for filtration to apparatus 3 and then to apparatus 4, where evaporation to a concentration of 45-50% occurs. The concentrate leaving the apparatus 3 is sent by stream III to the condensate accumulator.

To replenish the vitamins of groups A, B1-B12, C, D, E, K, P, H, one stripped off extract is fed into apparatus 6, the cold extract from intermediate tank 5 is also added thereto, while the mass ratio of cold extract to one stripped off is 4.0 -6.0 to 36.0. The finished mixture is fed to the filling apparatus 7, from where a 45-50% solution is sent by stream IV as the target product or freeze-drying to the apparatus 8 and then by stream V to the packaging.

In the finished concentrate, the pH value is determined by a standard method on an ionomer or pH meter, as well as biological activity, the content of micro and macro elements, the content of amino acids, organic acids, carbohydrates, vitamins are determined by known methods.

To determine the biological activity, the prepared extract in the amount of 0.25 L is evaporated to a volume of 50 ml, and the protein activity is determined in arbitrary units on a KFK-2 device.

Vitamins are determined by known methods according to GOST 70-47-55, GOST 52147-2003. Amino acid analysis and active protein are determined by reaction with sodium citron and two-coordinate thin-layer chromatography with the manifestation of ninhydril, and labels are introduced in the form of individual solutions of amino acids.

The composition of trace elements used, the conditions for the preparation of a biologically active preparation and its indicators are presented in tables.

Based on the data presented, it can be concluded that the biologically active preparation obtained by the claimed method differs in the composition of trace elements and their quantity, as well as in the quantitative composition of vitamins, amino acids; The method differs in the parameters of the process and the process itself, as a result of which the target product is obtained with increased biological activity, rich in micro and macro elements, vitamins and other valuable components.

Table 7 Obtaining a biologically active drug No. // No. 1st stage - holdd extraction 2nd stage - hot extraction The mass ratio of cold to hot extract Target product Temperature ° C Time hour The mass ratio of plant materials to condensate Temperature ° C Pressure, atm Time hour The exposure time of the hot extract at 100 ° C Biological activity, conventional The concentration of the amount of vitamins, g / 100 g The number of flavonoids, g / 100 g one 60 0.3 1: 6 110 1,5 0.6 0.2 4:36 34 0.836 1.43 2 70 0.5 1: 6 130 3.0 1,0 0.5 5:36 36 1,367 1.90 3 80 0.4 1: 6 140 4,5 1,2 0.8 6:36 46 1,720 2.04 four 60 0.4 1: 6 140 4,5 1,2 0.8 6:36 38 1,830 1.87 5 80 0.3 1: 6 110 1,5 1,0 0.2 5:36 45 2,100 2.46 6 70 0.5 1: 6 130 3.0 1,0 0.6 5:36 48 2,417 2.61 7 70 0.5 1: 6 130 3.0 1,2 0.8 6:36 47 2,326 2,39 8 60 0.5 1: 6 110 1,5 1,0 0.8 4:36 40 1,231 1.93

TABLE 7 ' p / p Name The content of macro- and microelements in the extract according to examples mg / kg one 2 3 four 5 6 7 8 I Macronutrients one Calcium 16.5 17.3 18,4 17.4 18.2 18.0 17.6 14.6 2 Potassium 19.0 19.0 21,4 21,4 22.6 22.5 19.6 18.6 3 Sodium 13.5 13.8 14.1 14.6 13.8 13.8 13.8 14.6 four Magnesium 1.4 1,6 0.8 1,6 1.8 1.7 1.7 2.0 5 Phosphorus 22.5 21.0 22.0 22.4 21.0 21.1 21,4 21.6 II Trace elements one Iron 0.6 0.8 0.8 0.6 0.8 0.5 0.3 0.6 2 Zinc 0.18 0.13 0.16 0.18 0.16 0.17 0.17 0.18 3 Tin 0.21 0.25 0.30 0.28 0.31 0.30 0.25 0.31 four Cobalt 0.22 0.26 0.25 0.24 0.28 0.28 0.20 0.20 5 Chromium 0.46 0.50 0.48 0.50 0.43 0.43 0.50 0.51 6 Vanadium 0.06 0.08 0.08 0.04 0.06 0.08 0.06 0.09 7 Manganese 0.20 0.30 0.20 0.30 0.10 0.06 0.08 0.40 8 Molybdenum 0,03 0.04 0.05 0.02 0,03 0.04 0.06 0.05 9 Copper 0.60 0.40 0.46 0.51 0.53 0.47 0.62 0.48

TABLE 8 The content of macro- and micronutrients in the finished product p / p Name The content of macro- and microelements in the finished product, according to examples, mg / 100 g one 2 3 four 5 6 7 8 one Calcium 2000 4000 4000 5000 6000 5000 5000 8000 2 Potassium 12000 3000 4000 4000 6000 5000 6000 12000 3 Sodium 4000 12000 5000 4000 6000 6000 5000 4000 four Magnesium 400 620 810 640 1450 1660 520 2000 5 Phosphorus 1400 4100 4400 5600 6100 8000 6400 5200 6 Iron one hundred 80 200 300 450 600 510 350 7 Zinc 180 200 600 450 520 600 1000 800 8 Tin 0.2 0.3 0.2 0.1 0.4 0.4 0.2 0.3 9 Cobalt 0.1 0.2 0.4 0.3 0.2 0.4 0.3 0.2 10 Chromium 1,0 1,0 1.4 1,6 2.0 1,2 2.0 1.4 eleven Vanadium 0.3 0.1 0.2 0.1 0.3 0.4 0.2 0.1 12 Manganese 0.06 0.08 0.06 0.08 0.20 0.30 0.40 0.32 13 Molybdenum 1,0 1,2 1.4 1.8 1,2 1,0 1,6 1.8 fourteen Copper 7.0 6.0 8.0 11.0 12.0 16,0 14.0 20,0

TABLE 9 The content of amino acids, lipids, proteins in the finished product p / p Name The content of the examples, mg / 100 g one 2 3 four 5 6 7 8 one Glycine 46 54 68 thirty 49 one hundred 110 130 2 Alanine 80 110 96 89 thirty 90 130 110 3 Valine one hundred 80 69 45 80 73 one hundred thirty four Leucine 40 thirty 70 thirty 80 71 46 54 5 Isoleucine 40 36 thirty 36 40 33 38 40 6 Cysteine 60 46 70 thirty 48 54 36 one hundred 7 Methionine thirty 60 70 40 thirty 40 one hundred twenty 8 Phenylalanine thirty 70 80 thirty 70 one hundred 120 twenty 9 Tyrosine twenty 80 90 one hundred 70 80 twenty one hundred 10 Tryptophan thirty 40 fifty twenty 80 70 one hundred twenty eleven Proline thirty fifty 60 twenty 90 80 one hundred twenty 12 Serine 40 fifty 70 twenty 80 70 twenty one hundred 13 Threonine thirty thirty 40 twenty thirty 60 80 twenty fourteen Asparagine twenty 25 thirty 26 fifty fifty 60 twenty fifteen Glutamine thirty 40 60 thirty 40 fifty twenty twenty 16 Aspartic acid twenty 90 110 80 80 110 170 200 17 Glutamic acid thirty 80 90 twenty 200 170 180 twenty eighteen Histidine twenty thirty 70 thirty one hundred 80 twenty 60 19 Lysine thirty thirty 40 twenty 40 fifty 60 twenty twenty Arginine thirty thirty 40 twenty 60 one hundred 80 60 21 Protein MM> 15 kDt, 20000 26000 30000 23000 25,000 28,000 30000 21000 22 Lipids (fats) 600 500 600 one hundred 700 1000 600 800

TABLE 10 The content of sugars, organic acids, flavones and flavonols, mg / 100 g in the finished product p / p Name The content of the examples, mg / 100 g one 2 3 four 5 6 7 8 one Arabinose 600 400 800 300 800 700 600 300 2 Xylose 500 400 800 300 600 1500 600 500 3 Fructose 4600 3000 4800 6000 4000 3600 5000 8000 four Galactose 610 300 480 850 620 580 1000 470 5 Glucose 3000 4100 7000 3100 4000 3400 5800 3800 6 Sucrose 600 1500 500 500 1000 800 400 400 7 Maltose 200 300 one hundred 500 400 600 400 500 8 Ribose 300 400 700 200 800 600 550 410 8 Aminosugar 4000 6300 2000 8000 6000 8000 4500 2000 9 Wine acid one hundred 400 300 300 400 300 400 one hundred 10 Lemon acid 300 700 400 300 300 500 700 300 eleven Vitamin C 400 300 300 400 200 300 350 300 12 Oxalic acid 300 fifty one hundred 60 300 400 400 200 13 Apple acid 300 400 500 600 400 300 300 800 fourteen Uronic acids 3000 5000 4000 8000 6000 7000 5000 6000 fifteen Humic acids 4000 6000 5000 7000 8000 3000 5000 4000 16 Flavones and flavonols 400 270 390 300 400 330 350 200

TABLE 11 The content of vitamins, mg or ME in 100 g of dry product p / p Name The vitamin content in the examples, mg / 100 g one 2 3 four 5 6 7 8 I Vitamin A (and retinol) 32 28 46 fifteen 80 67 46 53 one Vitamin B ₁ (thiamine) 43 37 21 42 fifteen 80 fifteen 41 2 Vitamin B2 (Riboflovin) 40 40 twenty one hundred 80 70 thirty fifty 3 Vitamin B3 (PP, Niacin) 300 500 200 800 700 500 400 300 four Vitamin B4 (Choline) twenty twenty 40 thirty fifty 40 twenty twenty 5 Vitamin B5 (pantothenic acid 200 500 300 70 600 430 390 400 6 Vitamin B6 (Pyridoxine) one hundred 160 80 twenty 200 one hundred 40 80 7 Vitamin B9 (Folic Acid) 40 thirty thirty 10 80 fifty thirty 10 8 Vitamin B12 (Cyanocobalomin) 4.0 6.0 8.0 1,0 15.0 3.0 4.0 3.0 9 Vitamin C (Ascorbic Acid) 70 80 70 60 70 80 120 70 10 Vitamin D (Ergocalceiferol) 8.0 4.0 1,0 1,2 7.0 10.0 10.0 10.0 eleven Vitamin E (tocopherol isomers) 160 200 80 300 250 200 170 230 12 Vitamin K (phyllohydroquinone, vicasol) 60 70 10 fifty 40 80 60 thirty 13 Vitamin H (Biotin) 12.0 8.2 1,5 4.8 3,5 20,0 11.0 8.0 fourteen Vitamin P (bioflavonoids) 16,0 1,5 18.0 13.0 20,0 14.0 14.0 15.0

Claims (3)

1. A biologically active drug based on alfalfa obtained by treating it with an extractant according to the method according to claim 2, characterized in that it contains ingredients in the following ratio, mg / 100 g: calcium - 2000-8000 potassium - 4000-12000 sodium - 4000-12000 magnesium - 400-2000 phosphorus - 1400-8000 iron - 80-600 zinc - 180-1000 tin - 0.1-0.4 cobalt - 0.1-0.4 chrome - 1.0-2.0 vanadium - 0.1-0.4 Manganese - 0.06-0.40 molybdenum - 1.0-1.8 copper - 6.0-20.0 essential amino acids, mg / 100 g of product: valine - 30-100 leucine - 30-80 isoleucine - 30-40 lysine - 20-60 methionine - 20-100 threonine - 20-80 tryptophan - 20-100 phenylalanine - 20-120, essential amino acids, mg / 100 g of product Alanine - 30-130 arginine - 20-100 glycine - 30-130 histidine - 20-100 tyrosine - 20-100 asparagine - 20-60 aspartic acid - 20-200 glutamine - 20-60 glutamic acid - 20-200 proline - 20-100 series - 20-100 cysteine - 30-100, protein 20000-30000 mg / 100 g lipids - 100-100 mg / 100 g Aminosugar - 2000-8000 mg / 100 g, and also: vitamins: Vitamin A (retinol) - 15-80 Vitamin B1 (thiamine) - 15-80 Vitamin B2 (Riboflavin) - 20-100 Vitamin B3 (PP, Niacin) - 200-800 Vitamin B4 (Choline) - 20-50 Vitamin B5 (pantothenic acid) - 70-600 Vitamin B6 (pyrodoxin) - 20-200 Vitamin B9 (Folic Acid) - 10-80 Vitamin B12 (Piancocobalomin) - 1.0-15.0 Vitamin C (ascorbic acid) - 60-120 Vitamin D (ergocalciferol) - 1.0-10.0 Vitamin E (tocopherol isomers) - 80-300 Vitamin K (phyllohydroquinone, vicasol) - 10-80 Vitamin H (Biotin) - 1.5-20.0 Vitamin P (bioflavonoids), - 1.5-20.0 organic acids: ascorbic - 200-400 wine - 100-400 oxalic - 50-400 apple - 300-800 lemon - 300-1500 carbohydrates: arabinose - 300-800 xylose - 300-1500 fructose - 3000-8000 galactose - 300-1000 glucose - 3000-7000 sucrose - 400-1500 maltose - 100-600 2. A method of obtaining a biologically active drug based on alfalfa, including grinding vegetable raw materials, treating it with an extractant containing an aqueous solution of salts of macro- and microelements, holding the extracting mixture at elevated temperature and elevated pressure, with further separation, purification and drying of the obtained extract, first, the extraction of plant materials is carried out with condensate heated to a temperature of 60-80 ° C for 0.3-0.5 hours, while the ratio of condensate to plant materials is 6: 1 wt., d A part of the extract is then transferred to an intermediate tank, and the rest is fed to “hot extraction” in a steam-heated apparatus into which aqueous solutions of macro- and microelements are introduced per 1 kg of plant material, and “hot extraction” is carried out at a temperature of 110 -140 ° C and a pressure of 1.5-4.5 atm for 0.6-1.2 hours, after which the excess pressure is released, and the temperature is reduced to 100 ° C, and at this temperature the mixture can withstand another 0.2- 0.8 h, then the resulting extract is fed to the filtration and evaporation, where evaporation occurs concentration to 45-50%, after which one stripped off extract is combined with a cold extract, while the mass ratio of cold extract to one stripped off is 4.0-6.0: 36.0, and then the finished mixture is served for bottling or freeze-drying and further to the packaging. 3. The method according to claim 2, characterized in that the amount of aqueous solutions of salts of macro- and microelements is served in the following ratio, mg / kg of plant material: Calcium - 14.6-18.4 Potassium - 18.6-22.6 Sodium - 13.5-14.6 Magnesium - 1.4-2.0 Phosphorus - 21.0-22.5 Iron - 0.3-0.8 Zinc - 0.13-0.18 Tin - 0.21-0.31 Cobalt - 0.20-0.28 Chrome - 0.43-0.51 Vanadium - 0.04-0.09 Manganese - 0.06-0.40 Molybdenum - 0.02-0.06 Copper - 0.40-0.62

Images