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RU2002111335A - ENVIRONMENT FOR CULTIVATION OF CELLS WITHOUT PROTEINS AND WITHOUT SERUM - Google Patents

ENVIRONMENT FOR CULTIVATION OF CELLS WITHOUT PROTEINS AND WITHOUT SERUM

Info

Publication number
RU2002111335A
RU2002111335A RU2002111335/13A RU2002111335A RU2002111335A RU 2002111335 A RU2002111335 A RU 2002111335A RU 2002111335/13 A RU2002111335/13 A RU 2002111335/13A RU 2002111335 A RU2002111335 A RU 2002111335A RU 2002111335 A RU2002111335 A RU 2002111335A
Authority
RU
Russia
Prior art keywords
medium
cells
soy hydrolyzate
medium according
protein
Prior art date
Application number
RU2002111335/13A
Other languages
Russian (ru)
Other versions
RU2266325C2 (en
Inventor
Манфред Райтер
Вольфганг Мундт
Фридрих ДОРНЕР
Леопольд Грилльбергер
Артур Миттерер
Original Assignee
Бакстер Акциенгезелльшафт
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT0165999A external-priority patent/AT409379B/en
Application filed by Бакстер Акциенгезелльшафт filed Critical Бакстер Акциенгезелльшафт
Publication of RU2002111335A publication Critical patent/RU2002111335A/en
Application granted granted Critical
Publication of RU2266325C2 publication Critical patent/RU2266325C2/en

Links

Claims (16)

1. Среда, не содержащая белков и не содержащая сыворотки, для культивирования клеток, включающая в себя гидролизат сои, где по меньшей мере 40% указанного гидролизата сои имеет молекулярную массу ≤500 Да.1. Medium-free protein and serum-free cell culture medium comprising soy hydrolyzate, where at least 40% of said soy hydrolyzate has a molecular weight of ≤500 Da. 2. Среда по п.1, где указанная среда содержит гидролизат сои в количестве, превышающем 10% по массе, исходя из общей массы сухой среды.2. The medium according to claim 1, where the specified medium contains soy hydrolyzate in an amount exceeding 10% by weight, based on the total weight of the dry medium. 3. Среда по п.1, где указанная среда содержит ультрафильтрованный гидролизат сои.3. The medium according to claim 1, where the specified medium contains an ultrafiltered soy hydrolyzate. 4. Среда по п.3, где указанная среда содержит очищенный гидролизат сои.4. The medium according to claim 3, where the specified medium contains purified soy hydrolyzate. 5. Среда по п.1, где гидролизат сои имеет содержание эндотоксина <500 ед./г.5. The medium according to claim 1, where the soy hydrolyzate has an endotoxin content of <500 units / g 6. Среда по п.1, где по меньшей мере 50% гидролизата сои имеет молекулярную массу ≤500 Да.6. The medium according to claim 1, where at least 50% of the soy hydrolyzate has a molecular weight of ≤500 Da. 7. Среда по п.6, где по меньшей мере 55% гидролизата сои имеет молекулярную массу ≤500 Да.7. The medium according to claim 6, where at least 55% of the soy hydrolyzate has a molecular weight of ≤500 Da. 8. Среда по п.1, где указанная среда также содержит аминокислоту.8. The medium according to claim 1, where the specified medium also contains an amino acid. 9. Среда по п.7, где указанная аминокислота выбрана из группы, состоящей из L-аспарагина, L-цистеина, L-цистина, L-пролина, L-триптофана, L-глутамина или их смесей.9. The medium of claim 7, wherein said amino acid is selected from the group consisting of L-asparagine, L-cysteine, L-cystine, L-proline, L-tryptophan, L-glutamine, or mixtures thereof. 10. Среда по п.1, где указанная среда также содержит дополнительные вещества, выбранные из группы, состоящей из буферных веществ, стабилизаторов окисления, стабилизаторов, противодействующих механическому воздействию, и ингибиторов протеаз.10. The medium according to claim 1, where the specified medium also contains additional substances selected from the group consisting of buffer substances, oxidation stabilizers, anti-mechanical stabilizers, and protease inhibitors. 11. Применение среды по п.1 для культивирования клеток млекопитающих.11. The use of the medium according to claim 1 for the cultivation of mammalian cells. 12. Применение среды по п.1 для культивирования клеток млекопитающих, выбранных из группы, состоящей из клеток СНО и клеток ВНК.12. The use of the medium according to claim 1 for the cultivation of mammalian cells selected from the group consisting of CHO cells and BHK cells. 13. Способ культивирования клеток, включающий в себя введение указанных клеток в среду по п.1 и выращивание указанных клеток в указанной среде.13. A method of culturing cells, comprising introducing said cells into the medium of claim 1 and growing said cells in said medium. 14. Способ получения белка из культуры клеток, который заключается во введении клеток в среду по п.1, причем указанные клетки экспрессируют требуемый белок, выращивании указанных клеток в указанной среде и экспрессии указанного белка, тем самым получении смеси указанных клеток и указанного белка в среде, очистке указанного белка из указанной смеси.14. A method of obtaining a protein from a cell culture, which consists in introducing cells into the medium according to claim 1, wherein said cells express the desired protein, growing said cells in said medium and expressing said protein, thereby obtaining a mixture of said cells and said protein in medium purifying said protein from said mixture. 15. Композиция культуры клеток, содержащая клетки млекопитающих и среду по п.1.15. The composition of the cell culture containing mammalian cells and the medium according to claim 1. 16. Способ приготовления среды для культивирования клеток по п.1, включающий в себя получение гидролизата сои, ультрафильтрацию указанного гидролизата сои с использованием способа ультрафильтрации, очистку указанной фракции гидролизата сои с использованием эксклюзионной хроматографии по размеру, отбор фракций гидролизата сои, состоящих из гидролизата сои, имеющего молекулярную массу ≤500 Да.16. The method of preparing the cell culture medium according to claim 1, comprising obtaining a soy hydrolyzate, ultrafiltrating said soy hydrolyzate using an ultrafiltration method, purifying said soy hydrolyzate fraction using size exclusion chromatography, selecting soy hydrolyzate fractions consisting of a soy hydrolyzate having a molecular weight of ≤500 Yes.
RU2002111335A 1999-09-28 2000-09-27 Cell cultivation medium without proteins and serum RU2266325C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0165999A AT409379B (en) 1999-06-02 1999-09-28 MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE
ATA1659/99 1999-09-28

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2005123274A Division RU2380412C2 (en) 1999-09-28 2000-09-27 Medium that does not contain proteins and serums, and method of mammal cell cultivation in such medium

Publications (2)

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RU2002111335A true RU2002111335A (en) 2003-11-10
RU2266325C2 RU2266325C2 (en) 2005-12-20

Family

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RU2002111335A RU2266325C2 (en) 1999-09-28 2000-09-27 Cell cultivation medium without proteins and serum
RU2005123274A RU2380412C2 (en) 1999-09-28 2000-09-27 Medium that does not contain proteins and serums, and method of mammal cell cultivation in such medium
RU2009131610/10A RU2536244C2 (en) 1999-09-28 2009-08-20 Medium for cultivation of cells without proteins and serum

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RU2005123274A RU2380412C2 (en) 1999-09-28 2000-09-27 Medium that does not contain proteins and serums, and method of mammal cell cultivation in such medium
RU2009131610/10A RU2536244C2 (en) 1999-09-28 2009-08-20 Medium for cultivation of cells without proteins and serum

Country Status (21)

Country Link
US (6) US20030203448A1 (en)
EP (1) EP1220893B2 (en)
JP (6) JP5441288B2 (en)
CN (2) CN101173246B (en)
AT (2) AT409379B (en)
AU (1) AU780791B2 (en)
CA (1) CA2385299A1 (en)
CY (1) CY1106135T1 (en)
CZ (1) CZ305307B6 (en)
DE (1) DE60028989T3 (en)
DK (1) DK1220893T4 (en)
ES (1) ES2265991T5 (en)
HU (1) HU228210B1 (en)
IL (4) IL148642A0 (en)
PL (1) PL215234B1 (en)
PT (1) PT1220893E (en)
RU (3) RU2266325C2 (en)
SI (1) SI1220893T1 (en)
SK (1) SK288059B6 (en)
TR (1) TR200200757T2 (en)
WO (1) WO2001023527A1 (en)

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