NO321650B1 - Fremgangsmate for okning av veksthastigheten til salmonid fisk, transgen salmonid fisk, analyse, promotersekvens, chimerisk genkonstruksjon og fiskevert. - Google Patents

Fremgangsmate for okning av veksthastigheten til salmonid fisk, transgen salmonid fisk, analyse, promotersekvens, chimerisk genkonstruksjon og fiskevert. Download PDF

Info

Publication number: NO321650B1
Authority: NO; Norway
Prior art keywords: fish; sequence; promoter; transgenic; gene
Prior art date: 1991-03-15

Application number

NO19933276A

Other languages

English (en)

Norwegian (no)

Other versions

NO933276L (no

NO933276D0 (no

Inventor

Choy L Hew

Garth L Fletcher

Original Assignee

Hsc Res Dev Lp

Seabridght Corp Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-03-15

Filing date

1993-09-14

Publication date

2006-06-19

1993-09-14 Application filed by Hsc Res Dev Lp, Seabridght Corp Ltd filed Critical Hsc Res Dev Lp

1993-09-14 Publication of NO933276D0 publication Critical patent/NO933276D0/no

1993-11-11 Publication of NO933276L publication Critical patent/NO933276L/no

2006-06-19 Publication of NO321650B1 publication Critical patent/NO321650B1/no

Links

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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates

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Biotechnology (AREA)
Biochemistry (AREA)
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Bioinformatics & Cheminformatics (AREA)
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Biomedical Technology (AREA)
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Proteomics, Peptides & Aminoacids (AREA)
Plant Pathology (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Veterinary Medicine (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Meat, Egg Or Seafood Products (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
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Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

NO19933276A 1991-03-15 1993-09-14 Fremgangsmate for okning av veksthastigheten til salmonid fisk, transgen salmonid fisk, analyse, promotersekvens, chimerisk genkonstruksjon og fiskevert. NO321650B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US66976591A	1991-03-15	1991-03-15
PCT/CA1992/000109 WO1992016618A1 (en)	1991-03-15	1992-03-12	Gene construct for production of transgenic fish

Publications (3)

Publication Number	Publication Date
NO933276D0 NO933276D0 (no)	1993-09-14
NO933276L NO933276L (no)	1993-11-11
NO321650B1 true NO321650B1 (no)	2006-06-19

Family

ID=24687639

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO19933276A NO321650B1 (no)	1991-03-15	1993-09-14	Fremgangsmate for okning av veksthastigheten til salmonid fisk, transgen salmonid fisk, analyse, promotersekvens, chimerisk genkonstruksjon og fiskevert.

Country Status (10)

Country	Link
US (1)	US5545808A (de)
EP (1)	EP0578653B1 (de)
JP (1)	JP3293622B2 (de)
AT (1)	ATE203269T1 (de)
AU (1)	AU669844B2 (de)
CA (2)	CA2367129A1 (de)
DE (1)	DE69231947T2 (de)
ES (1)	ES2163398T3 (de)
NO (1)	NO321650B1 (de)
WO (1)	WO1992016618A1 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6395959B1 (en)	1992-05-05	2002-05-28	Institut Pasteur	Nucleotide sequence encoding the enzyme I SceI and the use thereof
GB9216851D0 (en) *	1992-08-07	1992-09-23	Univ Manitoba	Dna sequences of rat probasin gene
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US5888981A (en)	1993-06-14	1999-03-30	Basf Aktiengesellschaft	Methods for regulating gene expression
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US5866755A (en) *	1993-06-14	1999-02-02	Basf Aktiengellschaft	Animals transgenic for a tetracycline-regulated transcriptional inhibitor
US5859310A (en)	1993-06-14	1999-01-12	Basf Aktiengesellschaft	Mice transgenic for a tetracycline-controlled transcriptional activator
US5912411A (en) *	1993-06-14	1999-06-15	University Of Heidelberg	Mice transgenic for a tetracycline-inducible transcriptional activator
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US5789655A (en)	1994-05-13	1998-08-04	The Regents Of The University Of California	Transgenic animals expressing artificial epitope-tagged proteins
CA2126138C (en) *	1994-06-17	2001-08-21	Robert H. Devlin	Transgenic fish and vectors therefor
US5907078A (en) *	1994-12-09	1999-05-25	Greenberg; Norman M.	Transgenic mouse model for prostate cancer
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US5986054A (en) *	1995-04-28	1999-11-16	The Hospital For Sick Children, Hsc Research And Development Limited Partnership	Genetic sequences and proteins related to alzheimer's disease
US6531586B1 (en)	1995-04-28	2003-03-11	The Hospital For Sick Children	Genetic sequences related to Alzheimer's Disease
US6210919B1 (en) *	1995-04-28	2001-04-03	Hsc Research And Development Limited Partnership	Genetic sequences and proteins related to alzheimer's disease
JP2000513923A (ja) *	1995-07-18	2000-10-24	ワシントン・ユニバーシティ	変異ｓ１８２遺伝子
WO1997009350A1 (en) *	1995-09-07	1997-03-13	Ppl Therapeutics (Scotland) Ltd.	Purification of alpha-1 proteinase inhibitor
US5780448A (en) *	1995-11-07	1998-07-14	Ottawa Civic Hospital Loeb Research	DNA-based vaccination of fish
JPH11511338A (ja) *	1996-01-31	1999-10-05	エイチエスシーリサーチアンドディヴェロップメントリミテッドパートナーシップ	細胞内不凍ポリペプチド又は核酸
US6333447B1 (en) *	1996-03-29	2001-12-25	The General Hospital Corporation	Transgenic model of heart failure
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DE69735498T2 (de)	1996-09-26	2006-11-09	The Regents Of The University Of Colorado, Boulder	Transgenes modell für herzversagen
GB9621113D0 (en) *	1996-10-10	1996-11-27	Univ Southampton	Transgenic fish
US5859314A (en) *	1996-10-18	1999-01-12	Ludwig Institute For Cancer Research	Mice with targeted tyrosine kinase, lyn, disruption
US6066778A (en) *	1996-11-06	2000-05-23	The Regents Of The University Of Michigan	Transgenic mice expressing APC resistant factor V
US6448470B1 (en) *	1997-02-25	2002-09-10	Thomas Jefferson University	Transgenic COL2A1-null mice expressing human COL2A1
US6380458B1 (en) *	1997-06-09	2002-04-30	Medical College Of Georgia Research Institute, Inc.	Cell-lineage specific expression in transgenic zebrafish
US7087420B1 (en)	1997-07-17	2006-08-08	Cambia	Microbial β-glucuronidase genes, gene products and uses thereof
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DE69926629T2 (de) *	1998-05-31	2006-06-08	University Of Georgia Research Foundation, Inc.	Transgene fische, die ein von bakteriophagen abgeleitetes transgenes konstrukt zum nachweis von mutationen tragen
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1992
- 1992-03-12 JP JP50579192A patent/JP3293622B2/ja not_active Expired - Lifetime
- 1992-03-12 CA CA002367129A patent/CA2367129A1/en not_active Abandoned
- 1992-03-12 WO PCT/CA1992/000109 patent/WO1992016618A1/en active IP Right Grant
- 1992-03-12 AT AT92906427T patent/ATE203269T1/de not_active IP Right Cessation
- 1992-03-12 AU AU13703/92A patent/AU669844B2/en not_active Expired
- 1992-03-12 EP EP92906427A patent/EP0578653B1/de not_active Expired - Lifetime
- 1992-03-12 DE DE69231947T patent/DE69231947T2/de not_active Expired - Fee Related
- 1992-03-12 CA CA002106315A patent/CA2106315C/en not_active Expired - Lifetime
- 1992-03-12 ES ES92906427T patent/ES2163398T3/es not_active Expired - Lifetime
1993
- 1993-09-14 NO NO19933276A patent/NO321650B1/no not_active IP Right Cessation
1994
- 1994-03-10 US US08/212,375 patent/US5545808A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE69231947T2 (de)	2002-05-29
EP0578653A1 (de)	1994-01-19
JP3293622B2 (ja)	2002-06-17
JPH06505870A (ja)	1994-07-07
CA2106315A1 (en)	1992-09-16
NO933276L (no)	1993-11-11
ATE203269T1 (de)	2001-08-15
US5545808A (en)	1996-08-13
CA2106315C (en)	2005-09-13
NO933276D0 (no)	1993-09-14
AU1370392A (en)	1992-10-21
CA2367129A1 (en)	1992-10-01
EP0578653B1 (de)	2001-07-18
AU669844B2 (en)	1996-06-27
DE69231947D1 (de)	2001-08-23
ES2163398T3 (es)	2002-02-01
WO1992016618A1 (en)	1992-10-01

Publication	Publication Date	Title
NO321650B1 (no)	2006-06-19	Fremgangsmate for okning av veksthastigheten til salmonid fisk, transgen salmonid fisk, analyse, promotersekvens, chimerisk genkonstruksjon og fiskevert.
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Noh et al.	2003	Genomic cloning of mud loach Misgurnus mizolepis (Cypriniformes, Cobitidae) β-actin gene and usefulness of its promoter region for fish transgenesis
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AU2001291520B2 (en)	2006-12-14	Genetic control of sex ratio in animal populations
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AU2001291520A1 (en)	2002-06-27	Genetic control of sex ratio in animal populations
INDE	0	Biotechnology in Aquaculture, with Special Reference to Transgenic Salmon
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Pandian	2005	Transgenesis in fishes: Indian research and development.

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