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MX351515B - Diferenciacion de celulas madre embrionarias humanas. - Google Patents

Diferenciacion de celulas madre embrionarias humanas.

Info

Publication number
MX351515B
MX351515B MX2012013156A MX2012013156A MX351515B MX 351515 B MX351515 B MX 351515B MX 2012013156 A MX2012013156 A MX 2012013156A MX 2012013156 A MX2012013156 A MX 2012013156A MX 351515 B MX351515 B MX 351515B
Authority
MX
Mexico
Prior art keywords
differentiation
stem cells
embryonic stem
human embryonic
cells
Prior art date
Application number
MX2012013156A
Other languages
English (en)
Other versions
MX2012013156A (es
Inventor
Jean Xu
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44912123&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX351515(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of MX2012013156A publication Critical patent/MX2012013156A/es
Publication of MX351515B publication Critical patent/MX351515B/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0613—Cells from endocrine organs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
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    • C07K—PEPTIDES
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
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Abstract

La presente invención se refiere a una población de células humanas del endodermo pancreático obtenible mediante cultivo de células que expresan marcadores característicos del linaje del endodermo definitivo con un medio suplementado con un activador de proteína quinasa C y un factor capaz de inhibir BMP o un inhibidor que señaliza un receptor de TGFß, caracterizada porque más del 90% de las células de la población coexpresa PDX1 y NXX6.1, y en donde células de la población expresan CDX2.
MX2012013156A 2010-05-12 2011-05-11 Diferenciacion de celulas madre embrionarias humanas. MX351515B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33383110P 2010-05-12 2010-05-12
PCT/US2011/036043 WO2011143299A2 (en) 2010-05-12 2011-05-11 Differentiation of human embryonic stem cells

Publications (2)

Publication Number Publication Date
MX2012013156A MX2012013156A (es) 2013-01-17
MX351515B true MX351515B (es) 2017-10-17

Family

ID=44912123

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012013156A MX351515B (es) 2010-05-12 2011-05-11 Diferenciacion de celulas madre embrionarias humanas.

Country Status (17)

Country Link
US (2) US9752125B2 (es)
EP (2) EP2569419B1 (es)
JP (4) JP6050225B2 (es)
KR (2) KR101903562B1 (es)
CN (2) CN102884176B (es)
AR (1) AR081040A1 (es)
AU (1) AU2011250912A1 (es)
BR (1) BR112012028855A2 (es)
CA (1) CA2800610C (es)
DK (1) DK2569419T3 (es)
ES (1) ES2728900T3 (es)
MX (1) MX351515B (es)
PH (2) PH12012502189B1 (es)
RU (2) RU2663339C1 (es)
SG (2) SG10201503652WA (es)
WO (1) WO2011143299A2 (es)
ZA (1) ZA201209383B (es)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
BRPI0814894A2 (pt) 2007-07-31 2014-10-21 Lifescan Inc Diferenciação de células-tronco embrionárias de ser humano.
KR101706928B1 (ko) 2007-11-27 2017-02-27 라이프스캔, 인코포레이티드 인간 배아 줄기 세포의 분화
CN105886459A (zh) 2008-02-21 2016-08-24 詹森生物科技公司 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物
ES2697798T3 (es) 2008-06-30 2019-01-28 Janssen Biotech Inc Diferenciación de células madre pluripotentes
US9234178B2 (en) 2008-10-31 2016-01-12 Janssen Biotech, Inc. Differentiation of human pluripotent stem cells
CA2742267C (en) 2008-10-31 2019-06-04 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
EP3363444B1 (en) 2008-11-14 2022-09-14 ViaCyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
PL2366021T3 (pl) 2008-11-20 2017-12-29 Janssen Biotech, Inc. Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach
CN102257132B (zh) 2008-11-20 2014-09-03 森托科尔奥索生物科技公司 用于在平面基底上进行细胞附着和培养的方法和组合物
JP6219568B2 (ja) 2009-07-20 2017-10-25 ヤンセン バイオテツク，インコーポレーテツド ヒト胚性幹細胞の分化
JP6392496B2 (ja) 2009-12-23 2018-09-19 ヤンセン バイオテツク，インコーポレーテツド ヒト胚性幹細胞の分化
RU2607380C2 (ru) 2010-03-01 2017-01-10 Янссен Байотек, Инк. Способы очистки клеток, производных от плюрипотентных стволовых клеток
MX351515B (es) 2010-05-12 2017-10-17 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
WO2012030538A2 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP2853589B1 (en) 2010-08-31 2017-12-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN103890167A (zh) 2011-06-21 2014-06-25 诺沃—诺迪斯克有限公司 自多潜能干细胞有效诱导定形内胚层
SG10201608914WA (en) 2011-12-22 2016-12-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
CA2866590A1 (en) 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
EP3450544A1 (en) 2012-06-08 2019-03-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endodrine cells
WO2014004341A1 (en) * 2012-06-26 2014-01-03 Seraxis, Inc. Stem cells and pancreatic cells useful for the treatment of insulin-dependent diabetes mellitus
JP6470687B2 (ja) * 2012-09-03 2019-02-13 ノヴォ ノルディスク アー／エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
GB201216796D0 (en) * 2012-09-20 2012-11-07 Cambridge Entpr Ltd In vitro pancreatic differentiation
RU2658488C2 (ru) * 2012-12-31 2018-06-21 Янссен Байотек, Инк. Способ получения клеток, экспрессирующих маркеры, характерные для панкреатических эндокринных клеток
SG11201505112SA (en) * 2012-12-31 2015-07-30 Janssen Biotech Inc Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
US10377989B2 (en) 2012-12-31 2019-08-13 Janssen Biotech, Inc. Methods for suspension cultures of human pluripotent stem cells
KR102361924B1 (ko) 2013-06-11 2022-02-14 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
EP2896688A1 (en) * 2014-01-20 2015-07-22 Centre National de la Recherche Scientifique (CNRS) A method of producing beta pancreatic cells from progenitor cells through the use of hydrogen peroxide
EP3143127B1 (en) 2014-05-16 2021-07-14 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
WO2016038038A1 (en) * 2014-09-08 2016-03-17 Fundacion Publica Andaluza Progreso Y Salud Method for obtaining pancreatic beta cell surrogates by increasing pancreatic and duodenal homeobox 1 (pdx-1) expression
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
US20160175363A1 (en) * 2014-12-18 2016-06-23 President And Fellows Of Harvard College Methods for generating stem cell-derived beta cells and uses thereof
ES2985135T3 (es) 2014-12-18 2024-11-04 Harvard College Procedimientos para generar células ß derivadas de células madre y usos de las mismas
CN113234661A (zh) 2014-12-18 2021-08-10 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
US10392598B2 (en) 2015-06-19 2019-08-27 Emory University Methods of measuring cell purity for making quality control determinations and related compositions
JP7123802B2 (ja) 2016-02-24 2022-08-23 ノヴォ ノルディスク アー／エス ヒト多能性幹細胞由来内分泌前駆細胞からの機能的ベータ細胞の産生
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
US20190390169A1 (en) * 2017-03-03 2019-12-26 Kyoto University Pancreatic progenitor cell production method
AU2018370029B2 (en) 2017-11-15 2024-11-07 Vertex Pharmaceuticals Incorporated Islet cell manufacturing compositions and methods of use
CA3108275A1 (en) 2018-08-10 2020-02-13 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
CA3139292A1 (en) 2019-05-31 2020-12-03 Timothy M. BRUHN Cell encapsulation devices with controlled oxygen diffusion distances
US20220370184A1 (en) 2019-05-31 2022-11-24 W. L. Gore & Associates, Inc. A biocompatible membrane composite
WO2020243663A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3186967A1 (en) 2020-07-31 2022-02-03 George Harb Differentiation of pancreatic endocrine cells

Family Cites Families (242)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US709392A (en) 1902-05-06 1902-09-16 Haydn Brown Suture-clamp.
US2232142A (en) 1940-09-27 1941-02-18 Schumann Seymour Wound clip
US2814296A (en) 1954-04-15 1957-11-26 S & R J Everett & Co Ltd Surgical needles
US3209652A (en) 1961-03-30 1965-10-05 Burgsmueller Karl Thread whirling method
AT326803B (de) 1968-08-26 1975-12-29 Binder Fa G Maschenware sowie verfahren zur herstellung derselben
US3935067A (en) 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
CA1201400A (en) 1982-04-16 1986-03-04 Joel L. Williams Chemically specific surfaces for influencing cell activity during culture
US4499802A (en) 1982-09-29 1985-02-19 Container Graphics Corporation Rotary cutting die with scrap ejection
US4537773A (en) 1983-12-05 1985-08-27 E. I. Du Pont De Nemours And Company α-Aminoboronic acid derivatives
US4557264A (en) 1984-04-09 1985-12-10 Ethicon Inc. Surgical filament from polypropylene blended with polyethylene
US5089396A (en) 1985-10-03 1992-02-18 Genentech, Inc. Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US5215893A (en) 1985-10-03 1993-06-01 Genentech, Inc. Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US4737578A (en) 1986-02-10 1988-04-12 The Salk Institute For Biological Studies Human inhibin
US5863531A (en) 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
CA1340581C (en) 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices
US5759830A (en) 1986-11-20 1998-06-02 Massachusetts Institute Of Technology Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo
US5567612A (en) 1986-11-20 1996-10-22 Massachusetts Institute Of Technology Genitourinary cell-matrix structure for implantation into a human and a method of making
US4898156A (en) 1987-05-18 1990-02-06 Mitek Surgical Products, Inc. Suture anchor
NZ229354A (en) 1988-07-01 1990-09-26 Becton Dickinson Co Treating polymer surfaces with a gas plasma and then applying a layer of endothelial cells to the surface
EP0363125A3 (en) 1988-10-03 1990-08-16 Hana Biologics Inc. Proliferated pancreatic endocrine cell product and process
US5837539A (en) 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
AU668349B2 (en) 1991-04-25 1996-05-02 Chugai Seiyaku Kabushiki Kaisha Reconstituted human antibody against human interleukin 6 receptor
US5449383A (en) 1992-03-18 1995-09-12 Chatelier; Ronald C. Cell growth substrates
GB9206861D0 (en) 1992-03-28 1992-05-13 Univ Manchester Wound healing and treatment of fibrotic disorders
CA2114282A1 (en) 1993-01-28 1994-07-29 Lothar Schilder Multi-layered implant
JP3525221B2 (ja) 1993-02-17 2004-05-10 味の素株式会社 免疫抑制剤
US5341922A (en) 1993-02-24 1994-08-30 Ethicon, Inc. Peelable foil suture packaging
AU687386B2 (en) 1993-04-08 1998-02-26 Human Cell Cultures, Inc. Cell culturing method and medium
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
GB9310557D0 (en) 1993-05-21 1993-07-07 Smithkline Beecham Plc Novel process and apparatus
TW257671B (es) 1993-11-19 1995-09-21 Ciba Geigy
US6703017B1 (en) 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US5834308A (en) 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US6001647A (en) 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US6083903A (en) 1994-10-28 2000-07-04 Leukosite, Inc. Boronic ester and acid compounds, synthesis and uses
ATE214602T1 (de) 1994-12-29 2002-04-15 Chugai Pharmaceutical Co Ltd Verstärker eines anti-tumoragens beinhaltend einen il-6 antagonisten
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5718922A (en) 1995-05-31 1998-02-17 Schepens Eye Research Institute, Inc. Intravitreal microsphere drug delivery and method of preparation
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
IL132318A0 (en) 1997-04-24 2001-03-19 Ortho Mcneil Pharm Inc Substituted imidazoles useful in the treatment of inflammatory diseases
DK1028737T3 (da) 1997-07-03 2007-08-13 Osiris Therapeutics Inc Humane mesenchymale stamceller fra perifert blod
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
DK1015576T3 (da) 1997-09-16 2005-08-29 Egea Biosciences Llc Fremgangsmåde til fuldstændig kemisk syntese og aggregering af gener og genomer
WO1999020741A1 (en) 1997-10-23 1999-04-29 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
US6372779B1 (en) 1997-12-29 2002-04-16 Ortho Pharmaceutical Corporation Anti-inflammatory compounds
ES2258329T3 (es) 1998-03-18 2006-08-16 Osiris Therapeutics, Inc. Celulas madre mesenquimaticas para la prevencion y tratamiento de respuestas inmunes en trasplantes.
MY132496A (en) 1998-05-11 2007-10-31 Vertex Pharma Inhibitors of p38
US6413773B1 (en) 1998-06-01 2002-07-02 The Regents Of The University Of California Phosphatidylinositol 3-kinase inhibitors as stimulators of endocrine differentiation
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6610540B1 (en) 1998-11-18 2003-08-26 California Institute Of Technology Low oxygen culturing of central nervous system progenitor cells
US6413556B1 (en) 1999-01-08 2002-07-02 Sky High, Llc Aqueous anti-apoptotic compositions
WO2000043500A2 (en) 1999-01-21 2000-07-27 Vitro Diagnostics, Inc. Immortalized cell lines and methods of making the same
US6815203B1 (en) 1999-06-23 2004-11-09 Joslin Diabetes Center, Inc. Methods of making pancreatic islet cells
US6333029B1 (en) 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue
US6306424B1 (en) 1999-06-30 2001-10-23 Ethicon, Inc. Foam composite for the repair or regeneration of tissue
AU7719300A (en) 1999-09-27 2001-04-30 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US6685936B2 (en) 1999-10-12 2004-02-03 Osiris Therapeutics, Inc. Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation
US20030082155A1 (en) 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
JP2003517592A (ja) 1999-12-13 2003-05-27 ザ スクリプス リサーチ インスティチュート 膵島αおよびβ前駆体の同定ならびに単離のためのマーカー
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6436704B1 (en) 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
CA2413910A1 (en) 2000-06-26 2002-12-27 Renomedix Institute Inc. Cell fractions containing cells capable of differentating into neural cells
CN100525768C (zh) 2000-10-23 2009-08-12 史密丝克莱恩比彻姆公司 新化合物
EP1345946B1 (en) 2000-12-08 2005-08-10 Ortho-McNeil Pharmaceutical, Inc. Macroheterocylic compounds useful as kinase inhibitors
MXPA03005140A (es) 2000-12-08 2004-10-15 Johnson & Johnson Compuestos de pirrolina sustituidos con indazolilo como inhibidores de cinasa.
US6599323B2 (en) 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
JP2005503759A (ja) 2001-01-24 2005-02-10 アメリカ合衆国 幹細胞の膵臓内分泌細胞への分化方法
ES2571219T3 (es) 2001-01-25 2024-09-23 The United States Of America Represented By The Sec Dep Of Health And Human Services Formulación de compuestos de ácido borónico
US6656488B2 (en) 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
US20050054102A1 (en) 2001-04-19 2005-03-10 Anna Wobus Method for differentiating stem cells into insulin-producing cells
EP1391505B1 (en) 2001-04-24 2009-01-28 Ajinomoto Co., Inc. Stem cells and method of separating the same
WO2002092756A2 (en) 2001-05-15 2002-11-21 Rappaport Family Institute For Research In The Medical Sciences Insulin producing cells derived from human embryonic stem cells
US6626950B2 (en) 2001-06-28 2003-09-30 Ethicon, Inc. Composite scaffold with post anchor for the repair and regeneration of tissue
KR100418195B1 (ko) 2001-07-05 2004-02-11 주식회사 우리기술 전력케이블의 다중절연진단장치 및 그 방법
GB0117583D0 (en) 2001-07-19 2001-09-12 Astrazeneca Ab Novel compounds
US7432104B2 (en) 2001-08-06 2008-10-07 Bresgen Inc. Methods for the culture of human embryonic stem cells on human feeder cells
US6617152B2 (en) 2001-09-04 2003-09-09 Corning Inc Method for creating a cell growth surface on a polymeric substrate
EP1298201A1 (en) 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
JP2005506074A (ja) 2001-10-18 2005-03-03 イクシオン・バイオテクノロジー・インコーポレーテッド 肝臓の幹細胞および前駆細胞の膵臓機能細胞への転換
ATE438708T1 (de) 2001-11-15 2009-08-15 Childrens Medical Center Verfahren zur isolierung, expansion und differenzierung fötaler stammzellen aus chorionzotte, fruchtwasser und plazenta und therapeutische verwendungen davon
KR100930139B1 (ko) 2001-12-07 2009-12-07 사이토리 테라퓨틱스, 인크. 처리된 리포애스퍼레이트 세포로 환자를 치료하기 위한시스템 및 방법
KR101089591B1 (ko) * 2001-12-07 2011-12-05 제론 코포레이션 인간 배아 줄기세포 유래의 섬세포
WO2003054169A1 (en) 2001-12-21 2003-07-03 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
IL162663A0 (en) 2001-12-28 2005-11-20 Cellartis Ab A method for the establishment of apluripotent human blastocyst-derived stem cell line
US20030162290A1 (en) 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells
AU2003231358A1 (en) 2002-04-17 2003-10-27 Otsuka Pharmaceutical Co., Ltd. METHOD OF FORMING PANCREATIC Beta CELLS FROM MESENCHYMAL CELLS
US20040161419A1 (en) 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
AU2003225295A1 (en) 2002-05-08 2003-11-11 Janssen Pharmaceutica N.V. Substituted pyrroline kinase inhibitors
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
EP1507848A4 (en) 2002-05-28 2005-11-23 Becton Dickinson Co EXPANSION AND TRANSDIFFERENCING OF HUMAN AZINUS CELLS
JP2005531609A (ja) 2002-06-05 2005-10-20 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ キナーゼ阻害剤としてのシスインドリル−マレイミド誘導体
GB0212976D0 (en) 2002-06-06 2002-07-17 Tonejet Corp Pty Ltd Ejection method and apparatus
CN1171991C (zh) 2002-07-08 2004-10-20 徐如祥 人神经干细胞的培养方法
US6877147B2 (en) 2002-07-22 2005-04-05 Broadcom Corporation Technique to assess timing delay by use of layout quality analyzer comparison
US7838290B2 (en) 2002-07-25 2010-11-23 The Scripps Research Institute Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith
CA2494040A1 (en) 2002-07-29 2004-02-05 Es Cell International Pte Ltd. Multi-step method for the differentiation of insulin positive, glucose
US20040063204A1 (en) 2002-08-14 2004-04-01 Lijun Yang Bone marrow cell differentiation
US7371576B2 (en) 2002-09-06 2008-05-13 Reneuron, Inc. CD56 positive human adult pancreatic endocrine progenitor cells
US9969977B2 (en) 2002-09-20 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US20040062753A1 (en) 2002-09-27 2004-04-01 Alireza Rezania Composite scaffolds seeded with mammalian cells
AU2003285172A1 (en) 2002-11-08 2004-06-03 The Johns Hopkins University Human embryonic stem cell cultures, and compositions and methods for growing same
US7144999B2 (en) 2002-11-23 2006-12-05 Isis Pharmaceuticals, Inc. Modulation of hypoxia-inducible factor 1 alpha expression
WO2004050827A2 (en) 2002-12-05 2004-06-17 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
ES2571355T3 (es) 2002-12-16 2016-05-24 Technion Res & Dev Foundation Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas
RU2359671C2 (ru) 2003-01-29 2009-06-27 Такеда Фармасьютикал Компани Лимитед Способ получения препарата с покрытием
MXPA05007883A (es) 2003-01-29 2005-09-21 Takeda Pharmaceutical Proceso para producir una preparacion recubierta.
US20070154981A1 (en) 2003-02-14 2007-07-05 The Board Of Trustees Of The Leland Stanford Junior University Insulin-producing cells derived from stem cells
US20070155661A1 (en) 2003-02-14 2007-07-05 The Board Of Trustees Of The Leland Standord Junior University Methods and compositions for modulating the development of stem cells
CA2520861A1 (en) 2003-03-27 2004-10-14 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway
WO2004090110A2 (en) 2003-03-31 2004-10-21 Bresagen Inc. Compositions and methods for the control, differentiation and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
US20090203141A1 (en) 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
US7413734B2 (en) 2003-06-27 2008-08-19 Ethicon, Incorporated Treatment of retinitis pigmentosa with human umbilical cord cells
IL161903A0 (en) 2003-07-17 2005-11-20 Gamida Cell Ltd Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs
ITRM20030395A1 (it) 2003-08-12 2005-02-13 Istituto Naz Per Le Malattie Infettive Lazz Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
US20050042595A1 (en) 2003-08-14 2005-02-24 Martin Haas Banking of multipotent amniotic fetal stem cells
US7157275B2 (en) 2003-08-15 2007-01-02 Becton, Dickinson And Company Peptides for enhanced cell attachment and growth
WO2005021728A2 (en) 2003-08-27 2005-03-10 Stemcells California, Inc. Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for these populations
US6939633B2 (en) 2003-09-17 2005-09-06 General Motors Corporation Fuel cell shutdown and startup using a cathode recycle loop
US7468391B2 (en) 2003-12-17 2008-12-23 Allergan, Inc. Methods for treating retinoid responsive disorders using selective inhibitors of CYP26A and CYP26B
US20060030042A1 (en) 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
WO2005116073A2 (en) 2004-04-27 2005-12-08 Cythera, Inc. Pdx1 expressing endoderm
CN109628371B (zh) 2003-12-23 2021-02-19 维亚希特公司 定形内胚层
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
AU2004309421B2 (en) 2003-12-23 2011-04-21 Viacyte, Inc. Definitive endoderm
TWI334443B (en) 2003-12-31 2010-12-11 Ind Tech Res Inst Method of single cell culture of undifferentiated human embryonic stem cells
US20050233446A1 (en) 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
US7794704B2 (en) 2004-01-23 2010-09-14 Advanced Cell Technology, Inc. Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
WO2005071066A1 (en) 2004-01-23 2005-08-04 Board Of Regents, The University Of Texas System Methods and compositions for preparing pancreatic insulin secreting cells
GB2441530B (en) 2004-02-12 2009-09-23 Univ Newcastle Stem Cells
WO2005080598A1 (ja) 2004-02-19 2005-09-01 Dainippon Sumitomo Pharma Co., Ltd. 体細胞核初期化物質のスクリーニング方法
JP2008500809A (ja) 2004-03-09 2008-01-17 ライフスキャン・インコーポレイテッド インスリン産生細胞を発生させるための方法
JP2007528226A (ja) 2004-03-10 2007-10-11 リージェンツ オブ ザ ユニヴァーシティ オブ カリフォルニア 胚幹細胞の増殖用組成物および方法
WO2005097980A2 (en) 2004-03-26 2005-10-20 Geron Corporation New protocols for making hepatocytes from embryonic stem cells
KR20070029681A (ko) 2004-04-01 2007-03-14 위스콘신 얼럼나이 리서어치 화운데이션 줄기 세포의 내배엽 및 이자 혈통으로의 분화
GB0410011D0 (en) * 2004-05-05 2004-06-09 Novartis Forschungsstiftung Neural cell differentiation method
JP5687816B2 (ja) 2004-07-09 2015-03-25 ヴィアサイト，インコーポレイテッド 胚体内胚葉を分化させるための因子を同定する方法
KR20070083559A (ko) 2004-08-13 2007-08-24 더 유니버시티 오브 죠지아 리서치 파운데이션, 인코포레이티드 인간 배아 줄기세포에서의 자가재생 및 분화를 위한 조성물및 방법
WO2006026473A2 (en) 2004-08-25 2006-03-09 University Of Georgia Research Foundation, Inc. METHODS AND COMPOSITIONS UTILIZING MYC AND GSK3ß TO MANIPULATE THE PLURIPOTENCY OF EMBRYONIC STEM CELLS
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
DK3196296T3 (en) 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells
GB2432846B (en) 2004-09-08 2009-12-30 Wisconsin Alumni Res Found Medium and culture of embryonic stem cells
WO2006083782A2 (en) 2005-01-31 2006-08-10 Es Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
ES2627419T3 (es) 2005-03-04 2017-07-28 Lifescan, Inc. Células estromales adultas derivadas del páncreas
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
DK1864132T3 (da) 2005-03-31 2012-07-16 Stemnion Inc Fra amnion stammende cellesammensætninger, fremgangsmåder til fremstilling deraf og anvendelser deraf
CN100425694C (zh) 2005-04-15 2008-10-15 北京大学 诱导胚胎干细胞向胰腺细胞分化的方法
ATE553198T1 (de) 2005-04-15 2012-04-15 Geron Corp Behandlung von krebs durch die kombinierte hemmung der proteasom- und telomeraseaktivitäten
US20080208351A1 (en) 2005-04-26 2008-08-28 Aarhus Universitet Biocompatible Material for Surgical Implants and Cell Guiding Tissue Culture Surfaces
JP4557797B2 (ja) 2005-05-20 2010-10-06 株式会社日立製作所 光ディスク装置
CA2610598A1 (en) 2005-06-10 2006-12-21 Irm Llc Compounds that maintain pluripotency of embryonic stem cells
WO2006138433A2 (en) 2005-06-14 2006-12-28 The Regents Of The University Of California Induction of cell differentiation by class i bhlh polypeptides
US20080199959A1 (en) 2005-06-21 2008-08-21 Ge Healthcare Bio-Sciences Ab Method For Cell Culture
JP5560391B2 (ja) 2005-06-22 2014-07-23 アステリアス バイオセラピューティクス インコーポレイテッド ヒト胚性幹細胞の懸濁培養法
JP5345388B2 (ja) 2005-06-30 2013-11-20 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ 環式アニリノ−ピリジノトリアジン
GB2443370A (en) 2005-07-29 2008-04-30 Australian Stem Cell Ct Ltd Compositions and methods for growth of pluripotent cells
US20080194021A1 (en) 2005-07-29 2008-08-14 Mays Robert W Use of a Gsk-3 Inhibitor to Maintain Potency of Culture Cells
WO2007025234A2 (en) 2005-08-26 2007-03-01 The Trustees Of Columbia University In The City Of New York Generation of pancreatic endocrine cells from primary duct cell cultures and methods of use for treatment of diabetes
JP2009506769A (ja) 2005-09-02 2009-02-19 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 間充織幹細胞誘導方法
GB2444686B (en) 2005-09-12 2010-08-25 Es Cell Int Pte Ltd Differentiation of pluripotent stem cells using p38 MAPK inhibitors or prostaglandins
WO2007047509A2 (en) 2005-10-14 2007-04-26 Regents Of The University Of Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
JP5404047B2 (ja) 2005-10-27 2014-01-29 ヴィアサイト，インコーポレイテッド Ｐｄｘ１発現背側及び腹側前腸内胚葉
JP5098028B2 (ja) 2005-12-13 2012-12-12 国立大学法人京都大学 核初期化因子
WO2007082963A1 (es) 2006-01-18 2007-07-26 Fundación Instituto Valenciano De Infertilidad Líneas de células madre embrionarias humanas y métodos para usar las mismas
SG10202109176RA (en) 2006-02-23 2021-09-29 Viacyte Inc Compositions and methods useful for culturing differentiable cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
AU2007224116B2 (en) 2006-03-02 2013-11-07 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
WO2007127927A2 (en) 2006-04-28 2007-11-08 Lifescan, Inc. Differentiation of human embryonic stem cells
US8685730B2 (en) 2006-05-02 2014-04-01 Wisconsin Alumni Research Foundation Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
WO2007130474A2 (en) 2006-05-02 2007-11-15 Wisconsin Alumni Research Foundation Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
US7964402B2 (en) 2006-05-25 2011-06-21 Sanford-Burnham Medical Research Institute Methods for culture and production of single cell populations of human embryonic stem cells
CN101541953A (zh) 2006-06-02 2009-09-23 佐治亚大学研究基金会 通过从人胚胎干细胞获得的定形内胚层细胞的分化得到胰和肝内胚层细胞及组织
WO2007143193A1 (en) 2006-06-02 2007-12-13 University Of Georgia Research Foundation, Inc. Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems
US8415153B2 (en) 2006-06-19 2013-04-09 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
CN100494359C (zh) 2006-06-23 2009-06-03 中日友好医院 神经干细胞三维立体培养体外扩增的方法
CA2656175C (en) 2006-06-26 2018-08-28 Lifescan, Inc. Conditioned media obtained from amniotic fluid-derived cells for pluripotent stem cell culture
US20080003676A1 (en) 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
AU2007270069B2 (en) 2006-07-06 2013-05-16 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
WO2008013664A2 (en) 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
KR101331510B1 (ko) 2006-08-30 2013-11-20 재단법인서울대학교산학협력재단 저농도의 포도당을 함유하는 인간 배아줄기세포용 배지조성물 및 이를 이용한 인간 배아 줄기세포로부터 인슐린생산 세포 또는 세포괴로 분화시키는 방법, 그리고그로부터 유도된 인슐린 생산 세포 또는 세포괴
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
US20080091234A1 (en) 2006-09-26 2008-04-17 Kladakis Stephanie M Method for modifying a medical implant surface for promoting tissue growth
AU2007311026B2 (en) 2006-10-17 2012-05-17 Stiefel Laboratories, Inc. Talarazole metabolites
US20100323442A1 (en) 2006-10-17 2010-12-23 Emmanuel Edward Baetge Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells
CA2666789C (en) 2006-10-18 2016-11-22 Yong Zhao Embryonic-like stem cells derived from adult human peripheral blood and methods of use
US20110151554A1 (en) 2006-11-09 2011-06-23 Akira Yuo Method for culturing and subculturing primate embryonic stem cell, as well as method for inducing differentiation thereof
CN101605891A (zh) * 2006-11-20 2009-12-16 奥克塔金尼有限责任公司 PrP基因的遗传学消融使用靶定启动子陷阱法生产作为治疗剂的无血清重组蛋白的细胞
TW200836749A (en) 2007-01-09 2008-09-16 Vioquest Pharmaceuticals Inc Compositions including triciribine and bortezomib and derivatives thereof and methods of use thereof
KR20090115142A (ko) 2007-01-30 2009-11-04 유니버시티 오브 조지아 리서치 파운데이션, 인코포레이티드 초기 중배엽 세포，내배엽 및 중배엽 계통의 생성에 유용한 중내배엽 세포의 안정한 집단 및 다능성 유주 세포（ｍｍｃ）
GB0703188D0 (en) 2007-02-19 2007-03-28 Roger Land Building Large scale production of stem cells
US7688908B2 (en) 2007-03-12 2010-03-30 Samsung Electronics Co., Ltd. System and method for processing wireless high definition video data using a shortened last codeword
WO2008148105A1 (en) 2007-05-25 2008-12-04 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
JP5738591B2 (ja) 2007-07-18 2015-06-24 ライフスキャン・インコーポレイテッドＬｉｆｅｓｃａｎ，Ｉｎｃ． ヒト胚幹細胞の分化
RU2010107181A (ru) 2007-07-31 2011-09-20 Лайфскен, Инк. (Us) Дифференцировка плюрипотентных стволовых клеток с использованием питающих клеток человека
BRPI0814894A2 (pt) 2007-07-31 2014-10-21 Lifescan Inc Diferenciação de células-tronco embrionárias de ser humano.
AU2008291930B2 (en) 2007-08-24 2014-04-17 Slotervaart Participaties Bv Compositions for the treatment of neoplastic diseases
WO2009061442A1 (en) 2007-11-06 2009-05-14 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells
KR101706928B1 (ko) 2007-11-27 2017-02-27 라이프스캔, 인코포레이티드 인간 배아 줄기 세포의 분화
SG154367A1 (en) 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
WO2009096049A1 (ja) 2008-02-01 2009-08-06 Kyoto University 人工多能性幹細胞由来分化細胞
WO2009101407A2 (en) 2008-02-11 2009-08-20 Cambridge Enterprise Limited Improved reprogramming of mammalian cells, and the cells obtained
CN105886459A (zh) 2008-02-21 2016-08-24 詹森生物科技公司 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物
JPWO2009110215A1 (ja) 2008-03-03 2011-07-14 独立行政法人科学技術振興機構 繊毛細胞の分化誘導方法
EP2479260B1 (en) 2008-03-17 2016-01-06 Agency For Science, Technology And Research Microcarriers for stem cell culture
DK2283117T3 (da) 2008-04-21 2014-01-20 Viacyte Inc Fremgangsmåde til oprensning af pancreatiske endodermceller afledt fra humane embryoniske stamceller
US8338170B2 (en) * 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US8728812B2 (en) * 2008-04-22 2014-05-20 President And Fellows Of Harvard College Compositions and methods for promoting the generation of PDX1+ pancreatic cells
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
EP2297319B1 (en) 2008-06-03 2015-10-07 Viacyte, Inc. Growth factors for production of definitive endoderm
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
ES2697798T3 (es) 2008-06-30 2019-01-28 Janssen Biotech Inc Diferenciación de células madre pluripotentes
DE102008032236A1 (de) 2008-06-30 2010-04-01 Eberhard-Karls-Universität Tübingen Isolierung und/oder Identifizierung von Stammzellen mit adipozytärem, chondrozytärem und pankreatischem Differenzierungspotential
US20100028307A1 (en) 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
CA2742267C (en) 2008-10-31 2019-06-04 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
US9234178B2 (en) 2008-10-31 2016-01-12 Janssen Biotech, Inc. Differentiation of human pluripotent stem cells
CN108342353B (zh) 2008-11-04 2022-01-11 韦尔赛特公司 干细胞聚集体悬浮组合物及其分化方法
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
EP3363444B1 (en) 2008-11-14 2022-09-14 ViaCyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
PL2366021T3 (pl) 2008-11-20 2017-12-29 Janssen Biotech, Inc. Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach
ES2633935T3 (es) 2008-12-05 2017-09-26 Inserm - Institut National De La Santé Et De La Recherche Médicale Método y medio para la diferenciación neural de células pluripotentes
US8507274B2 (en) * 2009-02-06 2013-08-13 President And Fellows Of Harvard College Compositions and methods for promoting the generation of definitive endoderm
EP2456859A4 (en) 2009-07-20 2015-03-18 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
JP6219568B2 (ja) 2009-07-20 2017-10-25 ヤンセン バイオテツク，インコーポレーテツド ヒト胚性幹細胞の分化
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
JP6392496B2 (ja) 2009-12-23 2018-09-19 ヤンセン バイオテツク，インコーポレーテツド ヒト胚性幹細胞の分化
WO2011108993A1 (en) 2010-03-02 2011-09-09 National University Of Singapore Culture additives to boost stem cell proliferation and differentiation response
WO2011123572A1 (en) 2010-03-31 2011-10-06 The Scripps Research Institute Reprogramming cells
US9234170B2 (en) 2010-04-25 2016-01-12 Mount Sinai School Of Medicine Generation of anterior foregut endoderm from pluripotent cells
MX351515B (es) 2010-05-12 2017-10-17 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
AU2011285531B2 (en) 2010-08-05 2015-04-30 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
CA2809305C (en) 2010-08-31 2019-06-11 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
MY177150A (en) 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013055834A2 (en) 2011-10-11 2013-04-18 The New York Stem Cell Foundation Er stress relievers in beta cell protection
SG10201608914WA (en) 2011-12-22 2016-12-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
US10519422B2 (en) 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
EP3450544A1 (en) 2012-06-08 2019-03-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endodrine cells
JP6602745B2 (ja) 2013-03-15 2019-11-06 ザ ジャクソン ラボラトリー 非胚性幹細胞の単離とその使用

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