MX2021001260A - Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. - Google Patents
Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.Info
- Publication number
- MX2021001260A MX2021001260A MX2021001260A MX2021001260A MX2021001260A MX 2021001260 A MX2021001260 A MX 2021001260A MX 2021001260 A MX2021001260 A MX 2021001260A MX 2021001260 A MX2021001260 A MX 2021001260A MX 2021001260 A MX2021001260 A MX 2021001260A
- Authority
- MX
- Mexico
- Prior art keywords
- enzymes
- methods
- present
- rebaudioside
- polypeptides
- Prior art date
Links
- 108700023372 Glycosyltransferases Proteins 0.000 title abstract 7
- 239000004383 Steviol glycoside Substances 0.000 title 1
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 title 1
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 title 1
- 229930182488 steviol glycoside Natural products 0.000 title 1
- 235000019411 steviol glycoside Nutrition 0.000 title 1
- 150000008144 steviol glycosides Chemical class 0.000 title 1
- 235000019202 steviosides Nutrition 0.000 title 1
- 102000051366 Glycosyltransferases Human genes 0.000 abstract 6
- 108010043934 Sucrose synthase Proteins 0.000 abstract 5
- 108090000790 Enzymes Proteins 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 239000000203 mixture Substances 0.000 abstract 3
- 229930188195 rebaudioside Natural products 0.000 abstract 2
- RPYRMTHVSUWHSV-CUZJHZIBSA-N rebaudioside D Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RPYRMTHVSUWHSV-CUZJHZIBSA-N 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 239000001512 FEMA 4601 Substances 0.000 abstract 1
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 abstract 1
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 abstract 1
- 235000019203 rebaudioside A Nutrition 0.000 abstract 1
- GSGVXNMGMKBGQU-PHESRWQRSA-N rebaudioside M Chemical compound C[C@@]12CCC[C@](C)([C@H]1CC[C@@]13CC(=C)[C@@](C1)(CC[C@@H]23)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)C(=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GSGVXNMGMKBGQU-PHESRWQRSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/252—Naphthacene radicals, e.g. daunomycins, adriamycins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Abstract
La presente invención proporciona enzimas de glucosiltransferasa (GT) modificadas con ingeniería genética, polipéptidos que tienen actividad de GT y polinucleótidos que codifican estas enzimas, así como vectores y células hospederas que comprenden estos polinucleótidos y polipéptidos; la presente invención proporciona enzimas de sacarosa sintasa (SuS) modificadas con ingeniería genética, polipéptidos que tienen actividad de SuS y polinucleótidos que codifican estas enzimas, así como vectores y células hospederas que comprenden estos polinucleótidos y polipéptidos; la presente invención también proporciona composiciones que comprenden las enzimas de GT y métodos de uso de las enzimas de GT modificadas con ingeniería genética para elaborar productos con enlaces ß-glucosa; la presente invención proporciona además composiciones y métodos para la producción de rebaudiósidos (por ejemplo, rebaudiósido M, rebaudiósido A, rebaudiósido I y rebaudiósido D); la presente invención también proporciona composiciones que comprenden las enzimas de SuS y métodos para usarlas; también se proporcionan métodos para producir las enzimas de GT y de SuS.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862712199P | 2018-07-30 | 2018-07-30 | |
| US201862712327P | 2018-07-31 | 2018-07-31 | |
| PCT/US2019/042151 WO2020028039A1 (en) | 2018-07-30 | 2019-07-17 | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021001260A true MX2021001260A (es) | 2021-04-12 |
Family
ID=69178045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021001260A MX2021001260A (es) | 2018-07-30 | 2019-07-17 | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US11760981B2 (es) |
| EP (1) | EP3830106A4 (es) |
| JP (4) | JP7584401B2 (es) |
| KR (1) | KR20210040408A (es) |
| CN (1) | CN112805295A (es) |
| AU (2) | AU2019314181B2 (es) |
| BR (1) | BR112021001661A8 (es) |
| CA (1) | CA3108268A1 (es) |
| IL (1) | IL280302A (es) |
| MX (1) | MX2021001260A (es) |
| SG (1) | SG11202100470WA (es) |
| TW (1) | TW202019951A (es) |
| WO (1) | WO2020028039A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3497222B1 (en) | 2016-08-12 | 2021-12-22 | Amyris, Inc. | Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides |
| CN111621482B (zh) * | 2020-06-30 | 2022-04-29 | 浙江工业大学 | 一种草铵膦脱氢酶突变体、基因工程菌及一锅法多酶同步定向进化方法 |
| WO2022002918A1 (en) | 2020-07-03 | 2022-01-06 | C-Lecta Gmbh | One-pot cell-free glycosylation process |
| CN112760302B (zh) * | 2020-12-23 | 2022-08-26 | 中化健康产业发展有限公司 | 一种能够催化莱鲍迪苷A生成莱鲍迪苷D的糖基转移酶StUGT |
| CN115418358B (zh) * | 2021-06-01 | 2024-01-30 | 弈柯莱生物科技(集团)股份有限公司 | 一种糖基转移酶及其应用 |
| US20240263152A1 (en) * | 2021-06-01 | 2024-08-08 | Abiochem Biotechnology Co., Ltd. | Glycosyltransferase and application thereof |
| CN115449514B (zh) * | 2021-06-08 | 2023-09-29 | 弈柯莱生物科技(上海)股份有限公司 | 一种β-1,2-糖基转移酶及其应用 |
| CN113462670B (zh) * | 2021-08-23 | 2023-03-24 | 兴化格林生物制品有限公司 | 一种糖基转移酶突变体及其催化合成莱鲍迪苷m的方法 |
| CN115725528B (zh) * | 2021-08-30 | 2024-01-12 | 弈柯莱生物科技(集团)股份有限公司 | 一种糖基转移酶及其应用 |
| CN116555210A (zh) * | 2022-01-30 | 2023-08-08 | 弈柯莱生物科技(上海)股份有限公司 | 糖基转移酶及其在制备莱鲍迪苷e中的应用 |
| CN119256080A (zh) * | 2022-05-23 | 2025-01-03 | 苏州引航生物科技有限公司 | 微生物来源的蔗糖合酶及其应用 |
| WO2024050533A2 (en) * | 2022-09-02 | 2024-03-07 | Arzeda Corp. | Compositions and methods for producing rebaudioside m |
| WO2024121721A1 (en) | 2022-12-05 | 2024-06-13 | Tate & Lyle Solutions Usa Llc | Methods for obtaining steviol glycosides |
Family Cites Families (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US20060257890A1 (en) | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| PT765394E (pt) | 1994-06-03 | 2002-03-28 | Novozymes Biotech Inc | Lacases myceliophthora purificadas e acidos nucleicos que as codificam |
| AU2705895A (en) | 1994-06-30 | 1996-01-25 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| FI104465B (fi) | 1995-06-14 | 2000-02-15 | Valio Oy | Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö |
| US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
| US6506602B1 (en) | 1996-03-25 | 2003-01-14 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6265201B1 (en) | 1997-01-17 | 2001-07-24 | Regents Of The University Of Minnesota | DNA molecules and protein displaying improved triazine compound degrading ability |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| DE69835360T2 (de) | 1997-01-17 | 2007-08-16 | Maxygen, Inc., Redwood City | EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| EP1036198B1 (en) | 1997-12-08 | 2012-09-26 | California Institute Of Technology | Method for creating polynucleotide and polypeptide sequences |
| WO1999051774A2 (en) | 1998-04-02 | 1999-10-14 | Tellus Genetic Resources, Inc. | A method for obtaining a plant with a genetic lesion in a gene sequence |
| NZ507591A (en) | 1998-05-01 | 2002-03-28 | Maxygen Inc | Optimization of pest resistance genes using DNA shuffling |
| EP1090024A2 (en) | 1998-06-17 | 2001-04-11 | Maxygen, Inc. | Method for producing polynucleotides with desired properties |
| US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
| EP1104459A1 (en) | 1998-08-12 | 2001-06-06 | Maxygen, Inc. | Dna shuffling of monooxygenase genes for production of industrial chemicals |
| CA2345203A1 (en) | 1998-10-07 | 2000-04-13 | Maxygen Inc. | Dna shuffling to produce nucleic acids for mycotoxin detoxification |
| EP1129184A1 (en) | 1998-11-10 | 2001-09-05 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes |
| JP4221100B2 (ja) | 1999-01-13 | 2009-02-12 | エルピーダメモリ株式会社 | 半導体装置 |
| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US8457903B1 (en) | 1999-01-19 | 2013-06-04 | Codexis Mayflower Holdings, Llc | Method and/or apparatus for determining codons |
| US7702464B1 (en) | 2001-08-21 | 2010-04-20 | Maxygen, Inc. | Method and apparatus for codon determining |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| US20070065838A1 (en) | 1999-01-19 | 2007-03-22 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7873477B1 (en) | 2001-08-21 | 2011-01-18 | Codexis Mayflower Holdings, Llc | Method and system using systematically varied data libraries |
| EP1072010B1 (en) | 1999-01-19 | 2010-04-21 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| JP2003524394A (ja) | 1999-02-11 | 2003-08-19 | マキシジェン， インコーポレイテッド | ハイスループット質量分析法 |
| EP1165757A1 (en) | 1999-03-05 | 2002-01-02 | Maxygen, Inc. | Encryption of traits using split gene sequences |
| US6703240B1 (en) | 1999-04-13 | 2004-03-09 | Maxygar, Inc. | Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6519065B1 (en) | 1999-11-05 | 2003-02-11 | Jds Fitel Inc. | Chromatic dispersion compensation device |
| US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
| SG121902A1 (en) | 2000-01-11 | 2006-05-26 | Maxygen Inc | Integrated systems for diversity generation and screening |
| WO2001075767A2 (en) | 2000-03-30 | 2001-10-11 | Maxygen, Inc. | In silico cross-over site selection |
| CA2405063C (en) | 2000-04-03 | 2013-06-04 | Maxygen, Inc. | Subtilisin variants |
| AU2002211798A1 (en) | 2000-10-20 | 2002-05-06 | Michigan State University | Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars |
| US20050084907A1 (en) | 2002-03-01 | 2005-04-21 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| US7783428B2 (en) | 2002-03-01 | 2010-08-24 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| US7747391B2 (en) | 2002-03-01 | 2010-06-29 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| WO2003078583A2 (en) | 2002-03-09 | 2003-09-25 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
| EP1654354A1 (en) | 2003-08-11 | 2006-05-10 | Codexis, Inc. | Improved ketoreductase polypeptides and related polynucleotides |
| WO2009015268A2 (en) * | 2007-07-24 | 2009-01-29 | Wisconsin Alumni Research Foundation | Engineered glycosyltransferases with expanded substrate specificity |
| HUE034642T2 (en) | 2008-02-12 | 2018-02-28 | Codexis Inc | A method for selecting an optimized diverse population of variants |
| WO2009102901A1 (en) | 2008-02-12 | 2009-08-20 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
| US8383346B2 (en) | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| CA2726850C (en) | 2008-06-13 | 2015-06-02 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| US20090312196A1 (en) | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| HUE042817T2 (hu) | 2011-06-28 | 2019-07-29 | Codexis Inc | Fehérjevariánsok elõállítása régiókeveréssel |
| WO2013159055A1 (en) | 2012-04-20 | 2013-10-24 | Codexis, Inc. | Production of fatty alcohols from engineered microorganisms |
| EP4012041A1 (en) | 2012-05-22 | 2022-06-15 | PureCircle SDN BHD | High-purity steviol glycosides |
| ES2979018T3 (es) | 2013-01-31 | 2024-09-23 | Codexis Inc | Métodos, sistemas y software para identificar biomoléculas usando modelos de forma multiplicativa |
| KR102536036B1 (ko) | 2014-10-03 | 2023-05-23 | 코나겐 인크. | 비칼로리 감미료 및 합성 방법 |
| AU2016250184B2 (en) * | 2015-04-14 | 2020-07-30 | Conagen Inc. | Production of non-caloric sweeteners using engineered whole-cell catalysts |
| WO2017207484A1 (en) | 2016-05-31 | 2017-12-07 | Universiteit Gent | Mutant sucrose synthases and their uses |
| CN109563493A (zh) * | 2016-06-15 | 2019-04-02 | 科德克希思公司 | 甜菊醇糖苷类和其他化合物用葡萄糖-1-磷酸酯的酶促糖基化 |
| US20180223264A1 (en) | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| MX2021000328A (es) * | 2018-07-09 | 2021-03-25 | Codexis Inc | Enzimas de variantes de galactosa oxidasa modificadas geneticamente. |
-
2019
- 2019-07-17 MX MX2021001260A patent/MX2021001260A/es unknown
- 2019-07-17 JP JP2021504769A patent/JP7584401B2/ja active Active
- 2019-07-17 CA CA3108268A patent/CA3108268A1/en active Pending
- 2019-07-17 US US16/514,578 patent/US11760981B2/en active Active
- 2019-07-17 EP EP19843463.1A patent/EP3830106A4/en active Pending
- 2019-07-17 CN CN201980064505.5A patent/CN112805295A/zh active Pending
- 2019-07-17 KR KR1020217006167A patent/KR20210040408A/ko active Pending
- 2019-07-17 AU AU2019314181A patent/AU2019314181B2/en active Active
- 2019-07-17 SG SG11202100470WA patent/SG11202100470WA/en unknown
- 2019-07-17 BR BR112021001661A patent/BR112021001661A8/pt unknown
- 2019-07-17 WO PCT/US2019/042151 patent/WO2020028039A1/en active Application Filing
- 2019-07-18 TW TW108125485A patent/TW202019951A/zh unknown
-
2021
- 2021-01-20 IL IL280302A patent/IL280302A/en unknown
-
2022
- 2022-07-15 JP JP2022113739A patent/JP7584473B2/ja active Active
- 2022-07-15 JP JP2022113740A patent/JP7584474B2/ja active Active
-
2023
- 2023-08-02 US US18/364,419 patent/US20240010999A1/en active Pending
-
2024
- 2024-06-28 JP JP2024105200A patent/JP2024114921A/ja active Pending
-
2025
- 2025-01-17 AU AU2025200365A patent/AU2025200365A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022132485A (ja) | 2022-09-08 |
| US20200032227A1 (en) | 2020-01-30 |
| AU2019314181B2 (en) | 2024-10-17 |
| IL280302A (en) | 2021-03-01 |
| EP3830106A4 (en) | 2022-07-06 |
| CN112805295A (zh) | 2021-05-14 |
| CA3108268A1 (en) | 2020-02-06 |
| AU2025200365A1 (en) | 2025-02-20 |
| EP3830106A1 (en) | 2021-06-09 |
| JP2024114921A (ja) | 2024-08-23 |
| BR112021001661A8 (pt) | 2022-08-09 |
| JP7584401B2 (ja) | 2024-11-15 |
| JP2021532757A (ja) | 2021-12-02 |
| AU2019314181A1 (en) | 2021-02-04 |
| BR112021001661A2 (pt) | 2021-05-04 |
| SG11202100470WA (en) | 2021-02-25 |
| US11760981B2 (en) | 2023-09-19 |
| WO2020028039A1 (en) | 2020-02-06 |
| JP7584473B2 (ja) | 2024-11-15 |
| JP7584474B2 (ja) | 2024-11-15 |
| TW202019951A (zh) | 2020-06-01 |
| KR20210040408A (ko) | 2021-04-13 |
| US20240010999A1 (en) | 2024-01-11 |
| JP2023039908A (ja) | 2023-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2021005862A (es) | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. | |
| MX2021001260A (es) | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. | |
| MX2019001631A (es) | Glicosiltransferasa dependiente de uridina difosfato para la produccion de alta eficiencia de rebaudiosidos. | |
| NZ725866A (en) | A non-caloric sweetener | |
| PH12018502655A1 (en) | Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate | |
| MX2021000605A (es) | Producción de glucósidos de esteviol a través de biotransformación de células enteras. | |
| MX2022000150A (es) | Enzima de glicosiltransferasa dependiente de difosfato de uridina. | |
| MX2017004248A (es) | Edulcorantes no caloricos y metodos para sintetizar. | |
| MX2019004368A (es) | Produccion biosintetica de glicosidos de esteviol y procesos de los mismos. | |
| MX2021013721A (es) | Produccion biosintetica del glucosido de esteviol rebaudiosido i a traves de enzimas variantes. | |
| MX2019010537A (es) | Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e. | |
| MX2023009628A (es) | Composiciones y metodos para producir rebaudiosido d. | |
| MY204670A (en) | Hydrolysis of steviol glycosides by beta-glucosidase | |
| MX2021015614A (es) | Enzimas variantes de sacarosa fosforilasa modificadas geneticamente. | |
| MX2018015534A (es) | Beta-glucosidasas dise?adas y metodos de glucosilacion. | |
| AR116249A1 (es) | Glicosiltransferasas diseñadas mediantes ingeniería y métodos de glucosilación de glicósidos de esteviol | |
| AU2016427120A8 (en) | Method for preparing rebaudioside C using enzymatic method | |
| AR110954A1 (es) | Métodos de glucosilación de glicosiltransferasas y glicósido de esteviol manipulados por ingeniería | |
| EA201991758A1 (ru) | Рекомбинантные гликозилтрансферазы и способы гликозилирования стевиоловых гликозидов | |
| PH12021552730A1 (en) | Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof |