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MX2020006117A - Integracion dirigida de acidos nucleicos. - Google Patents

Integracion dirigida de acidos nucleicos.

Info

Publication number
MX2020006117A
MX2020006117A MX2020006117A MX2020006117A MX2020006117A MX 2020006117 A MX2020006117 A MX 2020006117A MX 2020006117 A MX2020006117 A MX 2020006117A MX 2020006117 A MX2020006117 A MX 2020006117A MX 2020006117 A MX2020006117 A MX 2020006117A
Authority
MX
Mexico
Prior art keywords
targeted integration
nucleic acids
host cells
expression
producing
Prior art date
Application number
MX2020006117A
Other languages
English (en)
Inventor
Meixia Zhou
Chi Kin Domingos Ng
Amy Shen
Yongping Guo Crawford
Bradley R Snedecor
Shanhram Misaghi
Albert Eric Gao
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2020006117A publication Critical patent/MX2020006117A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La materia actualmente descrita se refiere a las células huésped de integración dirigida (TI) adecuadas para la expresión de proteínas recombinantes, así como también a los métodos para producir y usar dichas células huésped de TI.
MX2020006117A 2017-12-22 2018-12-21 Integracion dirigida de acidos nucleicos. MX2020006117A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762609806P 2017-12-22 2017-12-22
US201862711272P 2018-07-27 2018-07-27
PCT/US2018/067070 WO2019126634A2 (en) 2017-12-22 2018-12-21 Targeted integration of nucleic acids

Publications (1)

Publication Number Publication Date
MX2020006117A true MX2020006117A (es) 2022-02-10

Family

ID=65244601

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020006117A MX2020006117A (es) 2017-12-22 2018-12-21 Integracion dirigida de acidos nucleicos.

Country Status (13)

Country Link
US (2) US12129480B2 (es)
EP (1) EP3728309A2 (es)
JP (3) JP7557369B2 (es)
KR (2) KR102699853B1 (es)
CN (1) CN111886244A (es)
AU (1) AU2018392728A1 (es)
BR (1) BR112020012591A2 (es)
CA (1) CA3083579A1 (es)
IL (1) IL275462A (es)
MX (1) MX2020006117A (es)
SG (1) SG11202005109SA (es)
TW (1) TW201932600A (es)
WO (1) WO2019126634A2 (es)

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JP2024534389A (ja) 2021-09-15 2024-09-20 ジェネンテック， インコーポレイテッド メタボロミクス解析
CN118103517A (zh) 2021-10-18 2024-05-28 瑞泽恩制药公司 包括腺病毒相关病毒多核苷酸的真核细胞
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CA3239981A1 (en) * 2021-12-22 2023-06-29 Chi Kin Domingos Ng Multi-vector recombinase mediated cassette exchange
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CN117947069A (zh) * 2022-10-31 2024-04-30 深圳太力生物技术有限责任公司 稳定、高产的靶向整合细胞及其制备方法和应用
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Also Published As

Publication number Publication date
AU2018392728A1 (en) 2020-07-09
US12129480B2 (en) 2024-10-29
KR20240132119A (ko) 2024-09-02
CN111886244A (zh) 2020-11-03
BR112020012591A2 (pt) 2020-11-24
IL275462A (en) 2020-08-31
SG11202005109SA (en) 2020-07-29
JP2024095659A (ja) 2024-07-10
WO2019126634A2 (en) 2019-06-27
US20250179532A1 (en) 2025-06-05
KR102699853B1 (ko) 2024-08-30
JP2021507697A (ja) 2021-02-25
JP7557369B2 (ja) 2024-09-27
CA3083579A1 (en) 2019-06-27
TW201932600A (zh) 2019-08-16
KR20200103765A (ko) 2020-09-02
JP2022070949A (ja) 2022-05-13
EP3728309A2 (en) 2020-10-28
US20210002669A1 (en) 2021-01-07
WO2019126634A3 (en) 2019-08-15

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