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MX2015017110A - Integracion dirigida. - Google Patents

Integracion dirigida.

Info

Publication number
MX2015017110A
MX2015017110A MX2015017110A MX2015017110A MX2015017110A MX 2015017110 A MX2015017110 A MX 2015017110A MX 2015017110 A MX2015017110 A MX 2015017110A MX 2015017110 A MX2015017110 A MX 2015017110A MX 2015017110 A MX2015017110 A MX 2015017110A
Authority
MX
Mexico
Prior art keywords
targeted integration
cells
nucleic acid
acid sequence
exogenous nucleic
Prior art date
Application number
MX2015017110A
Other languages
English (en)
Inventor
Bahr Scott
Borgschulte Trissa
Kayser Kevin
Original Assignee
Sigma Aldrich Co Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co Llc filed Critical Sigma Aldrich Co Llc
Publication of MX2015017110A publication Critical patent/MX2015017110A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)

Abstract

La presente invención se refiere a una célula aislada que comprende una secuencia de ácido nucleico exógena localizada dentro de o próxima con un locus genómico predeterminado, en donde la secuencia de ácido nucleico exógena comprende al menos una secuencia de reconocimiento la cual puede ser explotada por una o más enzimas de modificación de polinucleótido para la integración dirigida de una proteína recombinante. La descripción proporciona además, métodos para preparar tales células, y métodos para retardar tales células para la producción de proteínas recombinantes, y kits para los mismos.
MX2015017110A 2013-06-19 2014-06-19 Integracion dirigida. MX2015017110A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361837019P 2013-06-19 2013-06-19
PCT/US2014/043138 WO2014205192A2 (en) 2013-06-19 2014-06-19 Targeted integration

Publications (1)

Publication Number Publication Date
MX2015017110A true MX2015017110A (es) 2016-08-03

Family

ID=52105507

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015017110A MX2015017110A (es) 2013-06-19 2014-06-19 Integracion dirigida.

Country Status (12)

Country Link
US (1) US20160145645A1 (es)
EP (1) EP3011011A4 (es)
JP (1) JP2016523084A (es)
KR (1) KR20160021812A (es)
CN (1) CN105555948A (es)
AU (1) AU2014281472A1 (es)
BR (1) BR112015031639A2 (es)
CA (1) CA2915467A1 (es)
MX (1) MX2015017110A (es)
RU (1) RU2016101246A (es)
SG (1) SG11201510297QA (es)
WO (1) WO2014205192A2 (es)

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US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
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WO2015188109A1 (en) 2014-06-06 2015-12-10 Regeneron Pharmaceuticals, Inc. Methods and compositions for modifying a targeted locus
EP4079847A1 (en) 2014-07-30 2022-10-26 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3521437B1 (en) 2014-11-21 2025-01-15 Regeneron Pharmaceuticals, Inc. Methods for targeted genetic modification using paired guide rnas
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US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
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Also Published As

Publication number Publication date
EP3011011A2 (en) 2016-04-27
EP3011011A4 (en) 2017-05-31
CN105555948A (zh) 2016-05-04
CA2915467A1 (en) 2014-12-24
BR112015031639A2 (pt) 2019-09-03
RU2016101246A3 (es) 2018-04-03
US20160145645A1 (en) 2016-05-26
JP2016523084A (ja) 2016-08-08
KR20160021812A (ko) 2016-02-26
RU2016101246A (ru) 2017-07-24
AU2014281472A1 (en) 2016-01-21
WO2014205192A2 (en) 2014-12-24
SG11201510297QA (en) 2016-01-28
WO2014205192A3 (en) 2015-03-19

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