[go: up one dir, main page]

MX2015016798A - Regulacion transcripcional guiada por acido ribonucleico. - Google Patents

Regulacion transcripcional guiada por acido ribonucleico.

Info

Publication number
MX2015016798A
MX2015016798A MX2015016798A MX2015016798A MX2015016798A MX 2015016798 A MX2015016798 A MX 2015016798A MX 2015016798 A MX2015016798 A MX 2015016798A MX 2015016798 A MX2015016798 A MX 2015016798A MX 2015016798 A MX2015016798 A MX 2015016798A
Authority
MX
Mexico
Prior art keywords
nucleic acid
rna
protein
cell
domain
Prior art date
Application number
MX2015016798A
Other languages
English (en)
Other versions
MX385330B (es
Inventor
George M Church
G Mali Prashant
M Esvelt Kevin
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52008736&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2015016798(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Publication of MX2015016798A publication Critical patent/MX2015016798A/es
Publication of MX385330B publication Critical patent/MX385330B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Lubricants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proveen métodos de modulación de la expresión de un ácido nucleico objetivo en una célula, que incluyen introducir en la célula un primer ácido nucleico ajeno que codifica uno o más ARN complementarios al ADN, en donde el ADN incluye el ácido nucleico objetivo, introducir en la célula un segundo ácido nucleico ajeno que codifica una proteína Cas9 nula de nucleasa que se une al ADN y es guiado por los uno o más ARN, introducir en la célula un tercer ácido nucleico ajeno que codifica una proteína o dominio regulador de transcripción, en donde son expresados los uno o más ARN, la proteína Cas9 nula de nucleasa y la proteína o dominio regulador de transcripción, en donde los uno o más ARN, la proteína Cas9 nula de nucleasa y la proteína o dominio regulador de transcripción se colocalizan en el ADN, y en donde la proteína o dominio regulador de transcripción regula la expresión del ácido nucleico objetivo.
MX2015016798A 2013-06-04 2014-06-04 Regulación transcripcional guiada por ácido ribonucleico. MX385330B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361830787P 2013-06-04 2013-06-04
PCT/US2014/040868 WO2014197568A2 (en) 2013-06-04 2014-06-04 Rna-guideded transcriptional regulation

Publications (2)

Publication Number Publication Date
MX2015016798A true MX2015016798A (es) 2016-10-26
MX385330B MX385330B (es) 2025-03-18

Family

ID=52008736

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015016798A MX385330B (es) 2013-06-04 2014-06-04 Regulación transcripcional guiada por ácido ribonucleico.
MX2021009672A MX2021009672A (es) 2013-06-04 2015-12-04 Regulacion transcripcional guiada por acido ribonucleico.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2021009672A MX2021009672A (es) 2013-06-04 2015-12-04 Regulacion transcripcional guiada por acido ribonucleico.

Country Status (14)

Country Link
US (2) US11981917B2 (es)
EP (3) EP3603679B1 (es)
JP (5) JP6621738B2 (es)
KR (3) KR102282990B1 (es)
CN (2) CN113846096B (es)
AU (4) AU2014274939B2 (es)
CA (2) CA2914638A1 (es)
ES (1) ES2930537T3 (es)
IL (3) IL284773B2 (es)
MX (2) MX385330B (es)
MY (2) MY197877A (es)
RU (2) RU2690935C2 (es)
SG (3) SG10201710030QA (es)
WO (1) WO2014197568A2 (es)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
EP4397760A3 (en) * 2012-10-23 2024-10-09 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
ES2658401T3 (es) 2012-12-12 2018-03-09 The Broad Institute, Inc. Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas
EP3434776A1 (en) 2012-12-12 2019-01-30 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
WO2014099750A2 (en) * 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
KR102282990B1 (ko) 2013-06-04 2021-07-28 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Rna-가이드된 전사 조절
US9267135B2 (en) 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
EP3004370B1 (en) * 2013-06-05 2024-08-21 Duke University Rna-guided gene editing and gene regulation
SG10201710487VA (en) 2013-06-17 2018-01-30 Broad Inst Inc Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for Targeting Disorders and Diseases Using Viral Components
WO2014204727A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
SG10201710486XA (en) 2013-06-17 2018-01-30 Broad Inst Inc Delivery and Use of the Crispr-Cas Systems, Vectors and Compositions for Hepatic Targeting and Therapy
WO2014204724A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2014204725A1 (en) * 2013-06-17 2014-12-24 The Broad Institute Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
EP3842528A1 (en) * 2013-09-18 2021-06-30 Kymab Limited Methods, cells and organisms
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
EP3080271B1 (en) 2013-12-12 2020-02-12 The Broad Institute, Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
KR20160097338A (ko) 2013-12-12 2016-08-17 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 반복 장애에서의 ｃｒｉｓｐｒ-ｃａｓ 시스템의 조성물 및 방법 및 용도
KR20250068794A (ko) 2013-12-12 2025-05-16 더 브로드 인스티튜트, 인코퍼레이티드 게놈 편집을 위한 ｃｒｉｓｐｒ-ｃａｓ 시스템 및 조성물의 전달, 용도 및 치료적 응용
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
AU2014361784A1 (en) 2013-12-12 2016-06-23 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR-Cas systems and compositions for HBV and viral diseases and disorders
US11028388B2 (en) 2014-03-05 2021-06-08 Editas Medicine, Inc. CRISPR/Cas-related methods and compositions for treating Usher syndrome and retinitis pigmentosa
ES2745769T3 (es) 2014-03-10 2020-03-03 Editas Medicine Inc Procedimientos y composiciones relacionados con CRISPR/CAS para tratar la amaurosis congénita de Leber 10 (LCA10)
US11141493B2 (en) 2014-03-10 2021-10-12 Editas Medicine, Inc. Compositions and methods for treating CEP290-associated disease
US11339437B2 (en) 2014-03-10 2022-05-24 Editas Medicine, Inc. Compositions and methods for treating CEP290-associated disease
WO2015139139A1 (en) * 2014-03-20 2015-09-24 UNIVERSITé LAVAL Crispr-based methods and products for increasing frataxin levels and uses thereof
EP3981876A1 (en) 2014-03-26 2022-04-13 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating sickle cell disease
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP4079847A1 (en) 2014-07-30 2022-10-26 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
DK3186376T3 (da) 2014-08-27 2019-05-06 Caribou Biosciences Inc Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet
CA2963693A1 (en) 2014-10-10 2016-04-14 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3215617B1 (en) 2014-11-07 2024-05-08 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
US10900034B2 (en) 2014-12-03 2021-01-26 Agilent Technologies, Inc. Guide RNA with chemical modifications
EP3889260A1 (en) 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
WO2016094874A1 (en) * 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
WO2016130600A2 (en) 2015-02-09 2016-08-18 Duke University Compositions and methods for epigenome editing
JP6873911B2 (ja) 2015-04-06 2021-05-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 初代細胞において標的核酸の遺伝子調節を誘導するためにインビトロで行う方法
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
SG11201708653RA (en) 2015-04-24 2017-11-29 Editas Medicine Inc Evaluation of cas9 molecule/guide rna molecule complexes
CA2986310A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
KR20230110368A (ko) 2015-06-01 2023-07-21 템플 유니버시티-오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 Hiv 감염의 rna-가이드된 치료를 위한 방법 및 조성물
KR102796744B1 (ko) 2015-06-09 2025-04-15 에디타스 메디신, 인코포레이티드 이식의 개선을 위한 crispr/cas-관련 방법 및 조성물
IL293323B2 (en) 2015-06-18 2024-01-01 Massachusetts Inst Technology CRISPR enzyme mutations that reduce unintended effects
WO2016205759A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
AU2016326711B2 (en) 2015-09-24 2022-11-03 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/Cas-mediated genome editing
CN108513575A (zh) 2015-10-23 2018-09-07 哈佛大学的校长及成员们 核碱基编辑器及其用途
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
MX2018005377A (es) 2015-11-30 2018-11-09 Univ Duke Dianas terapeuticas para la correccion del gen de la distrofina humana por edicion genica y metodos de uso.
WO2017106657A1 (en) 2015-12-18 2017-06-22 The Broad Institute Inc. Novel crispr enzymes and systems
WO2017127807A1 (en) * 2016-01-22 2017-07-27 The Broad Institute Inc. Crystal structure of crispr cpf1
EP3433364A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Systems and methods for treating alpha 1-antitrypsin (a1at) deficiency
EP3433363A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
EP4047092B1 (en) 2016-04-13 2025-07-30 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
WO2017189525A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
EP3907286A1 (en) * 2016-06-02 2021-11-10 Sigma-Aldrich Co., LLC Using programmable dna binding proteins to enhance targeted genome modification
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
EP3472321A2 (en) * 2016-06-17 2019-04-24 Genesis Technologies Limited Crispr-cas system, materials and methods
EP3487523B1 (en) 2016-07-19 2023-09-06 Duke University Therapeutic applications of cpf1-based genome editing
EP3494220A1 (en) 2016-08-02 2019-06-12 Editas Medicine, Inc. Compositions and methods for treating cep290 associated disease
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119010A1 (en) 2016-12-19 2018-06-28 Editas Medicine, Inc. Assessing nuclease cleavage
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
JP7229923B2 (ja) 2017-01-06 2023-02-28 エディタス・メディシン、インコーポレイテッド ヌクレアーゼ切断を評価する方法
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
WO2018170184A1 (en) 2017-03-14 2018-09-20 Editas Medicine, Inc. Systems and methods for the treatment of hemoglobinopathies
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
CA3058584A1 (en) 2017-03-30 2018-10-04 Kyoto University Method for inducing exon skipping by genome editing
CN116732106A (zh) 2017-04-20 2023-09-12 E开创生物技术股份有限公司 产生基因修改的动物的方法
EP3615672A1 (en) 2017-04-28 2020-03-04 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
EP3622070A2 (en) 2017-05-10 2020-03-18 Editas Medicine, Inc. Crispr/rna-guided nuclease systems and methods
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
AU2018279829B2 (en) 2017-06-09 2024-01-04 Editas Medicine, Inc. Engineered Cas9 nucleases
CN107266583B (zh) * 2017-06-27 2021-02-09 深圳大学 一种基于格氏嗜盐碱杆菌Argonaute靶向激活基因转录的方法
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
BR112020018364A2 (pt) 2018-03-14 2020-12-29 Editas Medicine, Inc. Sistemas e métodos para o tratamento de hemoglobinopatias
AU2019239880B2 (en) 2018-03-19 2023-11-30 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using CRISPR/Cas systems
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
CA3104856A1 (en) 2018-06-29 2020-01-02 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
GB201813011D0 (en) 2018-08-10 2018-09-26 Vib Vzw Means and methods for drought tolerance in crops
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
AU2020242032A1 (en) 2019-03-19 2021-10-07 Massachusetts Institute Of Technology Methods and compositions for editing nucleotide sequences
JP7525140B2 (ja) * 2020-01-31 2024-07-30 国立大学法人京都大学 タンパク質翻訳の制御システム
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
JP2024534945A (ja) 2021-09-10 2024-09-26 アジレント・テクノロジーズ・インク 化学修飾を有するプライム編集のためのガイドｒｎａ
WO2024193739A1 (en) * 2023-03-23 2024-09-26 Ostravska Univerzita Method for producing proteins in host cells

Family Cites Families (161)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123610A (en) 1977-03-09 1978-10-31 The United States Government Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4981985A (en) 1987-10-20 1991-01-01 Trustees Of The University Of Pennsylvania Synthesis of photolabile chelators for multivalent cations
US4844617A (en) 1988-01-20 1989-07-04 Tencor Instruments Confocal measuring microscope with automatic focusing
US5151189A (en) 1990-09-17 1992-09-29 Gelman Sciences, Inc. Cationic charge modified microporous membrane
JP3455217B2 (ja) 1992-02-13 2003-10-14 バイオ−メトリック システムズ インコーポレイテッド 架橋マトリックス中の化学種の固定
US5594235A (en) 1993-06-17 1997-01-14 Ultrapointe Corporation Automated surface acquisition for a confocal microscope
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US6284284B1 (en) 1995-06-06 2001-09-04 Advanced Tissue Sciences, Inc. Compositions and methods for production and use of an injectable naturally secreted extracellular matrix
US5830708A (en) 1995-06-06 1998-11-03 Advanced Tissue Sciences, Inc. Methods for production of a naturally secreted extracellular matrix
CZ397998A3 (cs) 1996-06-06 1999-07-14 Lynx Therapeutics, Inc. Způsob sekvenční analýzy ligací kódovaného adaptoru
US5948617A (en) 1997-06-13 1999-09-07 Biospeparations, Inc. Methods of in situ hybridization
US6083726A (en) 1998-02-03 2000-07-04 Lucent Technologies, Inc. Methods for polynucleotide synthesis and articles for polynucleotide hybridization
WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
EP1163369B1 (en) 1999-02-23 2011-05-04 Caliper Life Sciences, Inc. Sequencing by incorporation
EP1171636A2 (en) 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Assay of gene expression patterns by multi-fluor fish
US20020015952A1 (en) 1999-07-30 2002-02-07 Anderson Norman G. Microarrays and their manufacture by slicing
AU8015300A (en) 1999-10-12 2001-04-23 Ohio State University, The Reactive polymeric valve, dispensing devices and methods using same
AU1774501A (en) 1999-11-17 2001-05-30 Research Foundation Of State University Of New York, The Three dimensional data storage device and method for reading
AU2001247414A1 (en) 2000-03-14 2001-09-24 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US7033758B2 (en) 2000-06-02 2006-04-25 Bayer Corporation Highly sensitive gene detection and localization using in situ branched-DNA hybridization
EP1377679B1 (en) 2000-12-14 2009-10-28 Gen-Probe Incorporated Method and kit for enhancing the association rates of polynucleotides
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US20060248349A1 (en) 2001-09-24 2006-11-02 Rathjen Alicemarie G Method for preparing and using personal and genetic profiles
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
US20090246879A1 (en) 2002-12-20 2009-10-01 Callida Genomics Materials and Methods Relating to Nano-Tags and Nano-Brands
DE602004036672C5 (de) 2003-01-29 2012-11-29 454 Life Sciences Corporation Nukleinsäureamplifikation auf Basis von Kügelchenemulsion
CN103289893B (zh) 2003-02-26 2015-11-18 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
CN102135661B (zh) 2003-05-20 2014-06-04 卢西德有限公司 用于对患者身体所选位置进行成像的共焦显微镜
WO2005047543A2 (en) 2003-06-10 2005-05-26 Applera Corporation Ligation assay
US7193054B2 (en) 2003-08-26 2007-03-20 University Of Rochester Nanofabrication using actin filaments
EP1697535A2 (en) 2003-11-14 2006-09-06 University of Utah Research Foundation Methods, articles, and compositions for identifying oligonucleotides
US7381529B2 (en) 2003-12-31 2008-06-03 Intel Corporation Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20050191687A1 (en) 2004-02-27 2005-09-01 Tianxin Wang Method for multiplexed analyte detection
US7427479B2 (en) 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7253947B2 (en) 2004-10-07 2007-08-07 New York University Portable automated confocal microscope
DK1833840T3 (da) * 2004-11-09 2010-10-18 Santaris Pharma As Micromirs
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
ITRM20050068A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
US20060234261A1 (en) 2005-03-08 2006-10-19 Pierce Niles A Colorimetric readout of hybridization chain reaction
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US20070020650A1 (en) 2005-04-01 2007-01-25 Avak Kahvejian Methods for detecting proteins
MX2007012486A (es) 2005-04-08 2007-12-13 Mycosol Inc Ensayos de investigacion sobre estilbazio.
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
EP3492602A1 (en) 2005-06-15 2019-06-05 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
EP3042963A1 (en) 2005-06-20 2016-07-13 Advanced Cell Diagnostics, Inc. Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
CA2640385C (en) 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
US7864996B2 (en) 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
CA2647382A1 (en) 2006-03-10 2007-09-20 President And Fellows Of Harvard College Methods and apparatus for near field irradiation
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
ES2675047T3 (es) 2006-03-31 2018-07-06 Illumina, Inc. Sistemas para análisis de secuencia mediante síntesis
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US7964347B2 (en) 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
US20080038163A1 (en) 2006-06-23 2008-02-14 Applera Corporation Systems and Methods for Cooling in Biological Analysis Instruments
US7745129B1 (en) 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
US20100009868A1 (en) 2006-09-11 2010-01-14 The Arizona Board of Regents a body corporate acti ng for and behalf of Arizona State university Self-Assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing
US20090208965A1 (en) 2006-10-25 2009-08-20 Ikonisys, Inc. Automated method for detecting cancers and high grade hyperplasias
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
ES2541693T3 (es) 2007-03-02 2015-07-23 Dupont Nutrition Biosciences Aps Cultivos con resistencia mejorada a fagos
US8145677B2 (en) 2007-03-27 2012-03-27 Faleh Jassem Al-Shameri Automated generation of metadata for mining image and text data
EP2155770B1 (en) 2007-05-16 2013-10-16 California Institute of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US7939259B2 (en) 2007-06-19 2011-05-10 Stratos Genomics, Inc. High throughput nucleic acid sequencing by expansion
WO2009018576A1 (en) 2007-08-02 2009-02-05 Biodesic Compositions and methods for analyte detection and quantitation
EP2212434A1 (en) 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase ligation sequencing
KR20100089060A (ko) 2007-10-04 2010-08-11 할싸이언 몰레큘러 전자 현미경으로 핵산 중합체를 시퀀싱하는 방법
US20090139311A1 (en) 2007-10-05 2009-06-04 Applied Biosystems Inc. Biological Analysis Systems, Devices, and Methods
WO2009065093A2 (en) 2007-11-17 2009-05-22 University Of Medicine And Dentistry Of New Jersey Use of stem cells for wound healing
CN101586150B (zh) 2008-05-23 2016-09-28 陕西佰美基因股份有限公司 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途
US8546553B2 (en) 2008-07-25 2013-10-01 University Of Georgia Research Foundation, Inc. Prokaryotic RNAi-like system and methods of use
WO2010017264A2 (en) 2008-08-05 2010-02-11 Cornell University Photo-crosslinked nucleic acid hydrogels
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20100076057A1 (en) * 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
US20100087325A1 (en) 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
WO2010054108A2 (en) 2008-11-06 2010-05-14 University Of Georgia Research Foundation, Inc. Cas6 polypeptides and methods of use
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
JP4528345B2 (ja) 2009-01-28 2010-08-18 シャープ株式会社 動画再生装置、動画再生方法、動画再生方法をコンピュータで実現するためのプログラム及びそのプログラムを記録した記録媒体
FR2948475A1 (fr) 2009-07-24 2011-01-28 Bionext Procede de caracterisation d'objets tridimensionnels
US8431151B2 (en) 2009-08-06 2013-04-30 Syracuse University Antimicrobial nanostructured hydrogel web containing silver
JP5954876B2 (ja) 2009-10-13 2016-07-20 ナノストリング テクノロジーズ， インコーポレイテッド ナノレポーターによるタンパク質の検出
CN102712955A (zh) 2009-11-03 2012-10-03 Htg分子诊断有限公司 定量核酸酶保护测序(qNPS)
US9089512B2 (en) 2009-12-18 2015-07-28 President And Fellows Of Harvard College Active scaffolds for on-demand drug and cell delivery
US20110257031A1 (en) 2010-02-12 2011-10-20 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
US10267808B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
US10087431B2 (en) 2010-03-10 2018-10-02 The Regents Of The University Of California Methods of generating nucleic acid fragments
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
JP5893607B2 (ja) 2010-04-05 2016-03-23 プログノシス バイオサイエンシズ インコーポレイテッドＰｒｏｇｎｏｓｙｓ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． 空間コード化生物学的アッセイ
EP3078753B1 (en) 2010-05-10 2018-04-18 The Regents of The University of California Methods using endoribonuclease compositions
WO2011143583A1 (en) 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers
DK3360975T3 (da) 2010-07-09 2022-04-11 Cergentis B V Sekvenseringsstrategier for 3-D genomisk region af interesse
WO2012056440A1 (en) 2010-10-28 2012-05-03 Nanodoc Ltd. COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
WO2012067911A1 (en) 2010-11-16 2012-05-24 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
CN113151404A (zh) 2011-02-15 2021-07-23 莱卡生物系统纽卡斯尔有限责任公司 用于mrna的定位的原位检测的方法
JP5687514B2 (ja) 2011-02-17 2015-03-18 株式会社日立ハイテクノロジーズ 核酸配列解析装置
EP2500436B1 (en) 2011-03-17 2016-05-25 Institut Pasteur Method, probe and kit for DNA in situ hybridation and use thereof
DE102012008759A1 (de) 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-Triphosphat-Konjugate und Methoden zu deren Anwendung
CN103890191B (zh) 2011-05-27 2018-12-04 哈佛大学校长及研究员协会 单细胞全基因组扩增方法
WO2012164565A1 (en) 2011-06-01 2012-12-06 Yeda Research And Development Co. Ltd. Compositions and methods for downregulating prokaryotic genes
PL222511B1 (pl) 2011-06-08 2016-08-31 Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie Endorybonukleazy dsRNA
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
IN2014DN07853A (es) 2012-02-24 2015-04-24 Hutchinson Fred Cancer Res
GB2504240B (en) 2012-02-27 2015-05-27 Cellular Res Inc Compositions and kits for molecular counting of nucleic acids
HK1203566A1 (zh) 2012-02-29 2015-10-30 桑格摩生物科学股份有限公司 治療亨廷頓氏病的方法和組合物
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
CA2872241C (en) 2012-05-25 2022-12-06 The Regents Of The University Of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
SG196730A1 (en) 2012-07-16 2014-02-13 Agency Science Tech & Res Methods for reading data from a storage medium using a reader and storage devices
WO2014014106A1 (ja) 2012-07-20 2014-01-23 三菱化学メディエンス株式会社 光応答性核酸類を含むプローブを用いた光連結方法
EP2880171B1 (en) 2012-08-03 2018-10-03 The Regents of The University of California Methods and compositions for controlling gene expression by rna processing
AU2013302966B2 (en) 2012-08-15 2017-06-08 Lucid, Inc. Systems and methods for imaging tissue
CN102908119A (zh) 2012-09-26 2013-02-06 温州医学院眼视光研究院 一种共焦扫描成像系统及其像差控制方法
EP4397760A3 (en) * 2012-10-23 2024-10-09 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
ES2653212T3 (es) 2012-12-06 2018-02-06 Sigma Aldrich Co. Llc Modificación y regulación del genoma en base a CRISPR
ES2658401T3 (es) 2012-12-12 2018-03-09 The Broad Institute, Inc. Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
EP4286404A3 (en) * 2012-12-12 2024-02-14 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
PL2771468T3 (pl) 2012-12-12 2015-07-31 Broad Inst Inc Inżynieria systemów, metod i zoptymalizowanych kompozycji kierujących dla manipulacji sekwencjami
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP2931898B1 (en) 2012-12-12 2016-03-09 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
WO2014099750A2 (en) 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering
WO2014113493A1 (en) 2013-01-16 2014-07-24 Emory University Cas9-nucleic acid complexes and uses related thereto
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
ES2749624T3 (es) 2013-03-14 2020-03-23 Caribou Biosciences Inc Composiciones y métodos de ácidos nucleicos dirigidos a ácido nucleico
US9330295B2 (en) 2013-03-15 2016-05-03 Brown University Spatial sequencing/gene expression camera
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US20140273230A1 (en) 2013-03-15 2014-09-18 Sigma-Aldrich Co., Llc Crispr-based genome modification and regulation
JP6622183B2 (ja) 2013-03-15 2019-12-18 ザ ジェネラル ホスピタル コーポレイション ＲＮＡ誘導型ＦｏｋＩヌクレアーゼ（ＲＦＮ）を用いたＲＮＡ誘導型ゲノム編集の特異性の増大
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
FI2992115T4 (fi) 2013-04-30 2025-05-15 California Institute Of Technology Molekyylien limitetty leimaus sekventiaalisella hybridisaatioviivakoodauksella
EP3004349B1 (en) * 2013-05-29 2018-03-28 Cellectis S.A. A method for producing precise dna cleavage using cas9 nickase activity
US9267135B2 (en) * 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
KR102282990B1 (ko) 2013-06-04 2021-07-28 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Rna-가이드된 전사 조절
WO2014204725A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
US10655173B2 (en) 2013-10-18 2020-05-19 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
EP4219699A1 (en) 2013-12-12 2023-08-02 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
EP3108218A4 (en) 2014-02-21 2017-11-15 Massachusetts Institute Of Technology Expansion microscopy
CA2968376C (en) 2014-11-21 2020-06-23 Nanostring Technologies, Inc. Enzyme- and amplification-free sequencing
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
CN108700460B (zh) 2015-12-21 2020-11-03 威里利生命科学有限责任公司 成像系统和成像方法
WO2017161251A1 (en) 2016-03-17 2017-09-21 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
EP3943613B1 (en) 2016-07-05 2025-04-02 California Institute of Technology Fractional initiator hybridization chain reaction
EP3601339A4 (en) 2017-03-31 2020-11-04 Ultivue, Inc. DNA ANTIGEN EXCHANGE AND AMPLIFICATION

Also Published As

Publication number Publication date
KR102282990B1 (ko) 2021-07-28
AU2021221488A1 (en) 2021-09-16
SG11201509962QA (en) 2016-01-28
RU2019114706A (ru) 2019-10-11
JP7119055B2 (ja) 2022-08-16
CN113846096A (zh) 2021-12-28
US11981917B2 (en) 2024-05-14
KR20160014036A (ko) 2016-02-05
BR112015030491A2 (pt) 2017-07-25
IL302205B1 (en) 2025-04-01
AU2020203977A1 (en) 2020-07-02
KR20230042154A (ko) 2023-03-27
SG10201913068PA (en) 2020-02-27
MY177814A (en) 2020-09-23
US20230131972A1 (en) 2023-04-27
JP2024012387A (ja) 2024-01-30
CN105451778A (zh) 2016-03-30
AU2020203977B2 (en) 2021-05-27
EP4159243A1 (en) 2023-04-05
EP3603679A1 (en) 2020-02-05
NZ715280A (en) 2021-06-25
CN105451778B (zh) 2021-08-03
AU2014274939A1 (en) 2016-01-07
KR20210095235A (ko) 2021-07-30
MX2021009672A (es) 2021-09-08
MY197877A (en) 2023-07-22
IL302205A (en) 2023-06-01
JP2019122384A (ja) 2019-07-25
IL242959B (en) 2021-08-31
AU2014274939B2 (en) 2020-03-19
EP3603679B1 (en) 2022-08-10
BR112015030491A8 (pt) 2019-12-24
JP2016521554A (ja) 2016-07-25
JP2022166077A (ja) 2022-11-01
US20240175057A1 (en) 2024-05-30
MX385330B (es) 2025-03-18
EP3003392A4 (en) 2017-05-03
WO2014197568A2 (en) 2014-12-11
RU2019114706A3 (es) 2020-09-25
IL284773B1 (en) 2023-05-01
EP3003392B1 (en) 2019-10-23
RU2015156198A3 (es) 2018-05-08
EP3003392A2 (en) 2016-04-13
AU2024204479A1 (en) 2024-07-18
NZ753950A (en) 2021-09-24
CA2914638A1 (en) 2014-12-11
WO2014197568A3 (en) 2015-03-12
JP7376650B2 (ja) 2023-11-08
IL284773A (en) 2021-08-31
NZ753951A (en) 2021-09-24
CA3176690A1 (en) 2014-12-11
IL284773B2 (en) 2023-09-01
ES2930537T3 (es) 2022-12-16
CN113846096B (zh) 2025-05-09
JP2021045153A (ja) 2021-03-25
KR102512979B1 (ko) 2023-03-22
RU2756865C2 (ru) 2021-10-06
SG10201710030QA (en) 2018-01-30
RU2015156198A (ru) 2017-07-14
JP6621738B2 (ja) 2019-12-18
RU2690935C2 (ru) 2019-06-06
AU2021221488B2 (en) 2024-03-28
JP7036511B2 (ja) 2022-03-15

Similar Documents

Publication Publication Date Title
MX2015016798A (es) Regulacion transcripcional guiada por acido ribonucleico.
CY1124845T1 (el) Μεθοδοι παροχης μονοκλωνου rna
EA201791624A1 (ru) СОЕДИНЕНИЯ ДЛЯ УЛУЧШЕНИЯ СПЛАЙСИНГА мРНК
MX2021005226A (es) Regulacion de expresion genica mediada por nucleasa.
BR112018073861A2 (pt) métodos de terapia de genes para doenças e condições relacionadas com a idade
BR112019010565A2 (pt) aplicação viral de neoantígenos
HUE062646T2 (hu) CAR-módosított T-sejtek hatásának fokozása nukleinsav-vakcinációval
BR112016025519A2 (pt) engenharia genética in vivo com vetores de adenovírus
GB2544001A (en) Methods for increasing Cas9-mediated engineering efficiency
DOP2022000085A (es) Composiciones y métodos para inhibir la expresión del gen alas1
MX2016007541A (es) Moléculas modificadas de ácido nucleico y usos de las mismas.
MX368723B (es) Composiciones y metodos para inhibir la expresion del gen alas1.
AR093446A1 (es) Anticuerpos de antihemaglutinina y metodos de uso
MX375786B (es) Biosintesis microbiana de ergotioneina.
MX391773B (es) Variantes de lipasa y polinucleótidos que las codifican.
WO2014144942A3 (en) Dnai for the modulation of genes
MX2017004288A (es) Medio de induccion y metodos para el cultivo de y terapias con celulas madre.
MX2018007203A (es) Sistema de transposon, kit que comprende el mismo, y sus usos.
MX388883B (es) Mutaciones en proteinas del grupo hierro-azufre que mejoran la utilizacion de xilosa.
BR112016010159A2 (pt) usos de um conjunto de ácidos nucleicos e de um chip de microarranjo, chip de microarranjo, métodos para detecção de patógenos e/ou microrganismos benéficos em uma amostra orgânica e de tratamento de uma relva doente, e, kit
BR112018007901A2 (pt) produção biológica de metacrilato de metila
CO2021006679A2 (es) Métodos para formar poliplejos
EA201792106A1 (ru) Конструкция нуклеиновой кислоты для обеспечения толерантности к гербицидам у растений
EA201591444A1 (ru) Мутированные гены алленоксидсинтазы 2 (aos2)
UY34635A (es) ?acido nucleico de helianthus annuus modificado que mejora el rendimiento, vector, célula hospedante, planta transgénica y método de transformación?.