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KR960014327A - 세포 또는 고정화 세포 현탁액의 보존 방법 - Google Patents

세포 또는 고정화 세포 현탁액의 보존 방법 Download PDF

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KR960014327A
KR960014327A KR1019950035432A KR19950035432A KR960014327A KR 960014327 A KR960014327 A KR 960014327A KR 1019950035432 A KR1019950035432 A KR 1019950035432A KR 19950035432 A KR19950035432 A KR 19950035432A KR 960014327 A KR960014327 A KR 960014327A
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aqueous solution
cells
enzymatic activity
inorganic salt
activity
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KR1019950035432A
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KR100395275B1 (ko
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다카카즈 엔도
도시아키 도이
고지 다무라
유지 히라타
고조 무라오
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우에다케 이치로
닛토가가쿠고교 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier

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  • Inorganic Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

본 발명은 효소 활성을 지닌 미생물 세포를 이의 현택액으로서 또는 입자중의 고정화 세포의 현탁액으로서, 100mM 내지 무기 염의 포화 농도 범위의 몰 농도를 갖는, 무기 염의 중성 또는 약염기성 수용액인 수성 배지중에서, 상기 효소 활성을 지닌 세포 및 이의 효소 활성을 장시간 동안 안정하게 보존시키는 방법에 관한 것이다.
본 발명은 실온에서 조차도 세포 용해도 또는 효소 열화없이도 장시간(예 : 300일)동안 니트릴 하이드라타제 또는 니트릴라제 효소 활성을 갖는 다량의 세포 또는 입자중의 고정화 세포를 보존시키는 산업상 유용한 방법을 제공한다. 또한, 본 발명은 통상의 보존 방법에 필요한 노동력과 냉각비용을 상당히 감소시킬수 있게 한다.

Description

세포 또는 고정화 세포 현탁액의 보존 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (14)

  1. 효소 활성을 지닌 미생물 세포를 이의 현탁액으로서 또는 입자중에 고정화된 세포의 현탁액으로서, 100mM 내지 무기염의 포화 농도 범위의 몰 농도를 갖는, 무기 염의 중성 또는 약 염기성 수용액인 수성 배지내에 보존시킴을 포함하여, 상기 효소 활성을 지닌 미생물 세포 및 이의 효소 활성을 장시간 동안 안정하게 보존시키는 방법.
  2. 제1항에 있어서, 무기염의 수용액이 인산염, 붕산염, 황산염, 아황산염 및 염산염으로 이루어진 그룹중에서 선택된 하나 이상의 염의 수용액인 방법.
  3. 제1항에 있어서, 무기 염이 나트륨염, 칼륨염 및 암모늄염으로 이루어진 그룹 중에서 선택된 하나 이상의 것인 방법.
  4. 제1항에 있어서, 무기 염의 수용액의 pH 값이 pH 7 내지 10의 범위인 방법.
  5. 제1항에 있어서, 효소 활성이 니트릴 하이드라타제 활성 또는 니트릴라제 활성인 방법.
  6. 제1항에 있어서, 미생물 세포가 고르도나 테라에 MA-1(FERM BP-4535)이 방법.
  7. 제1항에 있어서, 미생물 세포가 로도코코스 로도크로우스종 HT40-6 (FERM BP-5231)인 방법.
  8. 제1항에 있어서, 미생물 세포가 로도코커스 로도크로우스 J-1 (FERM BP-1478)인 방법.
  9. 제1항에 있어서, 미생물 세포가 로도코커스 로도크로우스 ATCC 33278인 방법.
  10. 제1항에 있어서, 입자중에 고정화된 세포의 농도가 건조 세포를 기준으로 하여 0.1 내지 30중량%의 범위인 방법.
  11. 제1항에 있어서, 보존 온도 범위가 0 내지 40℃인 방법.
  12. 제1항에 있어서, 무기 염의 수용액이 300 내지 700mM 범위의 몰 농도를 갖는 인산염 완충액을 포함하는 방법.
  13. 제1항에 있어서, 무기 염의 수용액이 50mM의 몰 농도를 갖는 인산염의 완충액 및 300mM의 몰 농도를 갖는 황산나트륨 용액을 포함하는 방법.
  14. 제1항에 있어서, 입자중에 고정화된 세포를 황산암모늄 수용액중에 현탁시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950035432A 1994-10-14 1995-10-14 세포또는고정화세포현탁액의보존방법 KR100395275B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP94-274241 1994-10-14
JP27424194A JP3163224B2 (ja) 1994-10-14 1994-10-14 菌体または固定化菌体の懸濁液の保存方法

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KR960014327A true KR960014327A (ko) 1996-05-22
KR100395275B1 KR100395275B1 (ko) 2003-10-30

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US (1) US5705382A (ko)
EP (1) EP0707061B1 (ko)
JP (1) JP3163224B2 (ko)
KR (1) KR100395275B1 (ko)
CN (1) CN1214104C (ko)
DE (1) DE69518378T2 (ko)
TW (1) TW408179B (ko)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3163224B2 (ja) * 1994-10-14 2001-05-08 三菱レイヨン株式会社 菌体または固定化菌体の懸濁液の保存方法
US5858736A (en) * 1996-05-17 1999-01-12 E. I. Du Pont De Nemours And Company Preparation of lactams from aliphatic α,ω-dinitriles
US5863750A (en) * 1996-12-18 1999-01-26 Cytec Tech Corp Methods for the detoxification of nitrile and/or amide compounds
US6060265A (en) * 1996-12-18 2000-05-09 Cytec Technology Corporation Methods for the detoxification of nitrile and/or amide compounds
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
US6251646B1 (en) 1999-07-12 2001-06-26 E. I. Du Pont De Nemours And Company Method for stabilizing nitrilase activity and preserving microbial cells
US6551804B2 (en) * 1999-07-12 2003-04-22 E. I. Du Pont De Nemours And Company Process for preparing 4-cyanopentanoic acid
US6677149B2 (en) 1999-07-12 2004-01-13 E. I. Du Pont De Nemours And Company Method for stabilizing nitrilase activity and preserving microbial cells with carbamate salts
US6368804B1 (en) 1999-07-12 2002-04-09 E. I. Du Pont De Nemours & Company Method for stabilizing nitrilase activity and preserving microbial cells
KR100507248B1 (ko) 2000-03-21 2005-08-10 미쯔비시 레이온 가부시끼가이샤 미생물 배양 방법
US20050164902A1 (en) † 2003-10-24 2005-07-28 Ecolab Inc. Stable compositions of spores, bacteria, and/or fungi
ES2374617T3 (es) 2003-01-08 2012-02-20 Basf Se Método para la conservación y/o almacenamiento de células con actividad de nitrilasa o nitrilohidratasa.
JP4389064B2 (ja) 2003-05-28 2009-12-24 三井化学株式会社 ニトリルヒドラターゼ活性を維持または向上させる方法
RU2403280C2 (ru) 2003-12-02 2010-11-10 Циба Спешиалти Кемикэлз Уотер Тритментс Лимитед Штамм rhodococcus rhodochrous ncimb 41164 и его применение в качестве продуцента нитрилгидратазы
ATE449860T1 (de) * 2003-12-02 2009-12-15 Ciba Spec Chem Water Treat Ltd Herstellung von amiden
GB0327906D0 (en) * 2003-12-02 2004-01-07 Ciba Spec Chem Water Treat Ltd Biocatalyst composition
JP4435787B2 (ja) * 2003-12-16 2010-03-24 キアジェン ノース アメリカン ホールディングス，インコーポレイティド タンパク質を変性させるための処方物および方法
JP2006050930A (ja) * 2004-08-11 2006-02-23 Asahi Kasei Chemicals Corp ニトリラーゼ活性保持方法
CN101124321B (zh) 2004-11-05 2012-02-29 恰根北美控股有限公司 用于从稳定剂中纯化核酸的组合物和方法
US7943549B2 (en) * 2007-04-02 2011-05-17 Georgia State University Research Foundation, Inc. Biological-based catalyst to delay plant development processes
CN102010826B (zh) * 2009-07-24 2016-03-16 大野绿水株式会社 微生物菌体的保存方法及微生物菌体的混悬液
WO2013129179A1 (ja) 2012-02-28 2013-09-06 三菱レイヨン株式会社 酵素の保存方法
CA3156963A1 (en) * 2019-11-05 2021-05-14 Diego GHISLIERI Method of storing a biocatalyst

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1162484B (it) * 1978-03-29 1987-04-01 Nitto Chemical Industry Co Ltd Procedimento pe produrre acrilammide o metacrilammide impiegando microorganismi
JPS62257386A (ja) * 1986-05-02 1987-11-09 Nitto Chem Ind Co Ltd ニトリル水和活性の保持方法
JPS62259586A (ja) * 1986-05-02 1987-11-11 Nitto Chem Ind Co Ltd ニトリル水和活性の保持方法
MX169933B (es) * 1987-09-18 1993-08-02 Hideaki Yamada Procedimiento para la produccion biologica de amidas
WO1992002631A1 (en) * 1990-08-10 1992-02-20 Daicel Chemical Industries, Ltd. Process for producing optically active 3-phenyl-1,3-propanediol
SG48037A1 (en) * 1990-11-14 1998-04-17 Nitto Chemical Industry Co Ltd Biology process for production-alpha-hydroxyamide or alpha-hydroxy acid
JP2720140B2 (ja) * 1993-02-03 1998-02-25 日東化学工業株式会社 フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法
JP3354688B2 (ja) * 1994-01-28 2002-12-09 三菱レイヨン株式会社 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法
JP3163224B2 (ja) * 1994-10-14 2001-05-08 三菱レイヨン株式会社 菌体または固定化菌体の懸濁液の保存方法

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Publication number Publication date
CN1132788A (zh) 1996-10-09
JP3163224B2 (ja) 2001-05-08
TW408179B (en) 2000-10-11
CN1214104C (zh) 2005-08-10
DE69518378D1 (de) 2000-09-21
EP0707061A1 (en) 1996-04-17
KR100395275B1 (ko) 2003-10-30
EP0707061B1 (en) 2000-08-16
JPH08112089A (ja) 1996-05-07
DE69518378T2 (de) 2001-03-15
US5705382A (en) 1998-01-06

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