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KR950014136A - 당단백질의 분리 및 측정 방법 - Google Patents

당단백질의 분리 및 측정 방법 Download PDF

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Publication number
KR950014136A
KR950014136A KR1019940029963A KR19940029963A KR950014136A KR 950014136 A KR950014136 A KR 950014136A KR 1019940029963 A KR1019940029963 A KR 1019940029963A KR 19940029963 A KR19940029963 A KR 19940029963A KR 950014136 A KR950014136 A KR 950014136A
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South Korea
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antibody
glycoprotein
glycoproteins
lectin
measured
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KR1019940029963A
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KR100231628B1 (ko
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히데오 가또
겐지 나까무라
신지 사또무라
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다나까 모또아끼
와꼬쥰야꾸고오교 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57469—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving tumor associated glycolinkage, i.e. TAG
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57476—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving oncofetal proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/827—Lectins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Hospice & Palliative Care (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
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Abstract

질병에 의한 야기된 당단백질의 당 사슬중 구조적 변화도는 측정될 하나 이상의 당단백질 분해물의 특수한 당 사술구조를 인지할 수 있는 렉틴과, 모든 단백질 분해물에 대한 결합 특성을 가지지만 렉틴이 부착된 당단백질 분해물에는 결합되지 않는 제1항체의 배합물을 사용하고, 제1항체가 부착된 당단백질 분해물과 제1항체가 부착되어 있지 않은 당단백질을 이들간 성질의 차이를 이용하여 분리 및 측정함으로써, 당사슬 구조가 다르지만 실질적으로는 동일한 단백질 구조를 하는 두가지 이상의 형태를 가지는 당단백질을 분리 및 측정함에 의해 고 정밀도로 신속하게 측정할 수 있다.

Description

당단백질의 분리 및 측정 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 고압 액체 크로마토그래피(HPLC)에 의해 수득한 분리 패턴의 도면이다.
제3도는 실시예 1에서 사용한 HPLC장치의 개요를 나타낸 개요도이다.

Claims (7)

  1. 두개 이상의 형태를 가지는 당단백질을 포함하는 시료를 측정될 하나 이상의 당단백질의 특수한 당 사슬 구조를 인지할 수 있는 렉틴 및 모든 당단백질에 대한 결함특성을 가지지만 렉틴이 부착된 당 단백질에 대해서는 결합하지 않는 제1항체와 반응시킨 후, 제1항체가 부착된 당단백질과 제1항체가 부착되지 않은 당단백질을 이들 두 당단백질 간의 특성의 차이를 이용함으로써 분리 및 측정하는 것을 특징으로 하며, 당 사슬 구조가 다르지만 실질적으로는 동일한 단백질 구조를 가지는 분석물로서 둘 이상의 형태를 가지는 당단백질의 분리 및 측정방법.
  2. 제1항에 있어서, 측정될 당단백질과 제1항체의 복합물의 성질을 변화시킬 수 있는 물질 또는 표지(labeling)물질을 제1항체가 부착물질로서 가지고 있는 분리 및 측정방법.
  3. 제1항에 있어서, 렉틴이 부착된 당단백질을 포함하여 측정될 모든 당단백질에 대한 결합능력을 가지며, 표지 물질이 부착되어 있는 제2항체를 렉틴과 제1항체와 함께 사용하는 분리 및 측정방법.
  4. 제1항에 있어서, 측정될 당단백질을 글리코시다제로 예비 처리하거나, 당단백질을 렉틴 및 제1항체와 반응시킬 때 글리코시다제가 존재하는 분리 및 측정방법.
  5. 제3항에 있어서, 측정될 당단백질을 글리코시다제로 예비 처리하거나, 당단백질을 렉틴, 제1항체 및 제2항체와 반응시킬 때 글리코시다제가 존재하는 분리 및 측정방법.
  6. 제1항에 있어서, 성질 차이가 분자량, 등전점, 소수성 또는 전하량의 차이인 분리 및 측정방법.
  7. 제1항에 있어서, 렉틴을 당단백질의 고정 검색 제한 농도의 10배 이상인 농도로 사용하고, 제1항체의 농도가 (렉틴의 농도)×(렉틴의 결할 상수)/(제1항체의 결합 상수)이하인 분리 및 측정방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940029963A 1993-11-16 1994-11-15 당단백질의 분리 및 측정 방법 KR100231628B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP30983993 1993-11-16
JP93-309839 1993-11-16

Publications (2)

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KR950014136A true KR950014136A (ko) 1995-06-15
KR100231628B1 KR100231628B1 (ko) 1999-11-15

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Country Link
US (1) US5591589A (ko)
EP (1) EP0653640B1 (ko)
KR (1) KR100231628B1 (ko)
DE (1) DE69422692T2 (ko)
ES (1) ES2140511T3 (ko)
TW (1) TW380204B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8468646B2 (en) 2008-12-09 2013-06-25 Samsung Electronics Co., Ltd. Vacuum cleaner for using in both upright form and canister form

Families Citing this family (14)

* Cited by examiner, † Cited by third party
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EP0441470B1 (en) * 1990-01-09 1995-12-20 Wako Pure Chemical Industries Ltd Process for separating and measuring trace components
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
US6677123B1 (en) * 1998-02-03 2004-01-13 Wako Pure Chemical Industries, Ltd. Process for detecting increased risk of fetal chromosomal abnormality
DE69932893T2 (de) * 1998-06-30 2007-04-12 Wako Pure Chemical Industries, Ltd. Methode zur Messung von Thyroglobulin
DE60236262D1 (de) * 2001-04-04 2010-06-17 Wako Pure Chem Ind Ltd Elektrophorese mit doppelstrang-dna-marker
JPWO2002101389A1 (ja) * 2001-06-12 2005-04-07 和光純薬工業株式会社 ヒアルロン酸の測定方法
GB0212391D0 (en) * 2002-05-29 2002-07-10 Axis Shield Asa Assay
DE10249608A1 (de) * 2002-10-18 2004-05-06 Gkss-Forschungszentrum Geesthacht Gmbh Vorrichtung und Verfahren zur Strukturanalyse und Detektion von komplexen Glykostrukturen
ITTO20070455A1 (it) * 2007-06-25 2008-12-26 Univ Pisa Procedimento di determinazione e di separazione di isoforme di gamma-glutamiltransferasi (ggt) sierica in un campione di fluido biologico e isoforme enzimatiche cosi' ottenute
JP5413544B1 (ja) * 2012-04-27 2014-02-12 コニカミノルタ株式会社 酵素処理工程を含むレクチンを用いた抗原検出方法
US10352927B2 (en) 2012-08-10 2019-07-16 National Institute Of Advanced Industrial Science And Technology Glycoform detection method and glycoform detection device
CN103674918A (zh) * 2013-12-12 2014-03-26 复旦大学 一种基于凝集素液相悬浮芯片检测糖蛋白糖链结构的方法
US10371704B2 (en) 2013-12-27 2019-08-06 Konica Minolta, Inc. Method for discriminating between prostate cancer and a benign prostate disease
US10222385B2 (en) 2013-12-27 2019-03-05 Konica Minolta, Inc. Methods for discriminating between prostate cancer and a benign prostate-disease

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US4371515A (en) * 1978-12-26 1983-02-01 E-Y Laboratories, Inc. Method for forming an isolated lectin-immunological conjugate
CH645728A5 (fr) * 1979-01-30 1984-10-15 Otsuka Pharma Co Ltd Procede de determination de la teneur en substances contenant des liaisons glucosidiques associees aux tumeurs, application de ce procede et necessaire pour sa mise en oeuvre.
JPH0734015B2 (ja) * 1988-04-25 1995-04-12 和光純薬工業株式会社 微量成分の新規測定法
EP0399464A3 (en) * 1989-05-24 1992-03-25 Eiji Ishikawa Assay method for a substance with a specific sugar chain
EP0441470B1 (en) * 1990-01-09 1995-12-20 Wako Pure Chemical Industries Ltd Process for separating and measuring trace components

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8468646B2 (en) 2008-12-09 2013-06-25 Samsung Electronics Co., Ltd. Vacuum cleaner for using in both upright form and canister form

Also Published As

Publication number Publication date
KR100231628B1 (ko) 1999-11-15
TW380204B (en) 2000-01-21
ES2140511T3 (es) 2000-03-01
EP0653640B1 (en) 2000-01-19
EP0653640A1 (en) 1995-05-17
DE69422692T2 (de) 2000-07-06
US5591589A (en) 1997-01-07
DE69422692D1 (de) 2000-02-24

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