[go: up one dir, main page]

KR920704118A - 스캐닝 분광 광도계에서 시약 전달을 모니터하는 방법 - Google Patents

스캐닝 분광 광도계에서 시약 전달을 모니터하는 방법

Info

Publication number
KR920704118A
KR920704118A KR1019920701276A KR920701276A KR920704118A KR 920704118 A KR920704118 A KR 920704118A KR 1019920701276 A KR1019920701276 A KR 1019920701276A KR 920701276 A KR920701276 A KR 920701276A KR 920704118 A KR920704118 A KR 920704118A
Authority
KR
South Korea
Prior art keywords
dye
reagent
concentration
reaction mixture
composition
Prior art date
Application number
KR1019920701276A
Other languages
English (en)
Other versions
KR100210736B1 (ko
Inventor
코르넬리우스 드리스콜 리챠드
제이. 피셔 티모시
Original Assignee
에프.지.엠.헤르만스, 에이.지.제이.베르미렌
악조 엔. 브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에프.지.엠.헤르만스, 에이.지.제이.베르미렌, 악조 엔. 브이. filed Critical 에프.지.엠.헤르만스, 에이.지.제이.베르미렌
Publication of KR920704118A publication Critical patent/KR920704118A/ko
Application granted granted Critical
Publication of KR100210736B1 publication Critical patent/KR100210736B1/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Plasma & Fusion (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

내용 없음.

Description

스캐닝 분광 광도계에서 시약 전달을 모니터하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도에는 본 발명을 실시함에 있어서 유용한 염료인 페이턴트 블루(Patent Blue) VF의 스펙트럼이 나타나 있다. 페이턴트 블루 VF(설퍼 블루, CI 42045)는 도면에 예시된 바와 같이 635nm에서 흡수 피이크를 나타내며 410nm에서 두번째 피이크를 나타낸다,
제2도는 PT 응혈시간 반응에 사용되는 트롬보플라스틴에 있어서의 페이틴트 블루 VF를 나타낸다. 제2a도는 형성된 응혈에 대한 피이크를 나타내며 제2b도는 시약에서 염료와의 동일한 반응을 나타낸다.

Claims (21)

  1. 반응 혼합물내의 시약의 농도를 측정하는 방법으로서, 시약내에서 연료의 농도가 주어진 농도에 이를때까지 염료를 시약에 첨가하는 단계; 반응 혼합물을 생성하기 위해. 상기 시약과 반응하여 반응 생성물을 형성할 수 있는 성분을 포함하는 시료와 상기 시약을 혼합하는 단계; 첫번째 분광지역에서 반응 생성물이 형성되었음을 측정하는 단계; 염료가 광학적 특성을 나타내며, 첫번째 분광지역과는 다른 두 번째 분광지역에서 반응혼합물내의 염료 농도를 측정하는 단계; 및 측정된 엄료 농도를 기준으로하여 반응 혼합물내의 시약의 농도를 측정하는 단계를 포함하는 방법.
  2. 제1항에 있어서, 상기 반응 생성물 및 염료가 아무런 광학적 특성을 나타내지 않는 3번째 분광지역에서 반응 혼합물의 투과도를 측정하는 단계를 추가로 포함하는 것을 특징으로하는 방법.
  3. 제1항에 있어서, 상기 염료는 2번째 분광 지역에서 빛을 흡수함을 특징으로 하는 방법.
  4. 제3항에 있어서, 상기 염료는 첫번째 분광지역에서는 빛을 거의 흡수하지 않음을 특징으로 하는 방법.
  5. 제2항에 있어서, 상기 염료 및 반응 생성물은 3번째 분광지역에서 빛을 거의 홉수하지 않음을 특징으로 하는 방법.
  6. 제1항에 있어서, 상기 시약은 트롬보플라스틴을 포함하며 혈장을 포함하는 샘플을 사용함을 특징으로 하는 방법.
  7. 제1항에 있어서, 상기 시약은 트롭빈은 포함하며 혈장을 포함하는 샘플을 사용함을 특징으로 하는 방법.
  8. 제1항에 있어서, 상기 시약은 CaC12를 포함하며 혈장을 포함하는 샘플을 사용함을 특징으로 하는 방법.
  9. 제1항에 있어서, 상기 염료는 페이턴트 블루 VF를 포함하며 2번째 분광지역은 약 635nm의 지역임을 특징으로 하는 방법.
  10. 제1항에 있어서, 상기 염료는 2번째 분광지역에서 빛을 형광시킴을 특징으로 하는 방법.
  11. 제8항에 있어서, 상기 염료는 로다민 B를 포함함을 특징으로 하는 방법.
  12. 제1항에 있어서, 상기 첨가된 반응 혼합물대로 샘플이 첨가되었는지의 여부를, 반응 혼합물 내의 염료의 농도를 기준으로 하여 측정하는 단계를 더 포함함을 특징으로 하는 방법.
  13. 제1항에 있어서, 상기 염료는 시약의 약 1/1000부의 농도로 첨가됨을 특징으로 하는 방법.
  14. 약 400 내지 500nm 범위이의의 지역에서 광학적 특성을 나타내는 염료를 함유하는 혈액 응집 시약을 포함하는 조성물.
  15. 제14항에 있어서, 상기 혈액 응집 시약은 트롬빈을 포함함을 특징으로 하는 조성물.
  16. 제14항에 있어서, 상기 혈액 응집 시약은 트롬보플라스틴을 포함함을 특징으로 하는 조성물.
  17. 제14항에 있어서, 상기 혈액 응집 시약은 CaC12를 포함함을 특징으로 하는 조성물.
  18. 제14항에 있어서, 상기 염료는 약 400 내지 550nm의 범위 이의의 지역에서 빛을 흡수함을 특징으로 하는 조성물.
  19. 제14항에 있어서, 상기 염료는 약 400 내지 550nm범위 이의의 지역에서 형광을 나타냄을 특징으로 하는 조성물.
  20. 제14항에 있어서, 상기 염료는 페이턴트 블루 VF를 포함함을 특징으로 하는 조성물.
  21. 제19항에 있어서, 상기 염료는 로다민 B를 포함함을 특징으로 하는 조성물.
    ※참고사항:최초출원 내용에 의하여 공개되는 것임.
KR1019920701276A 1989-12-01 1990-12-03 스캐닝 분광 광도계에서 시약 전달을 모니터하는 방법 KR100210736B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/443,953 US5068181A (en) 1989-12-01 1989-12-01 Method of monitoring reagent delivery in a scanning spectrophotometer
US443953 1989-12-01
US443,953 1989-12-01
PCT/US1990/007068 WO1991008461A1 (en) 1989-12-01 1990-12-03 Method of monitoring reagent delivery in a scanning spectrophotometer

Publications (2)

Publication Number Publication Date
KR920704118A true KR920704118A (ko) 1992-12-19
KR100210736B1 KR100210736B1 (ko) 1999-07-15

Family

ID=23762872

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019920701276A KR100210736B1 (ko) 1989-12-01 1990-12-03 스캐닝 분광 광도계에서 시약 전달을 모니터하는 방법

Country Status (14)

Country Link
US (1) US5068181A (ko)
EP (1) EP0502983B1 (ko)
JP (1) JP2902108B2 (ko)
KR (1) KR100210736B1 (ko)
AT (1) ATE142018T1 (ko)
AU (2) AU655577B2 (ko)
CA (1) CA2069887C (ko)
DE (1) DE69028312T2 (ko)
DK (1) DK0502983T3 (ko)
ES (1) ES2095310T3 (ko)
FI (1) FI101575B1 (ko)
GR (1) GR3021261T3 (ko)
IE (1) IE75698B1 (ko)
WO (1) WO1991008461A1 (ko)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646046A (en) * 1989-12-01 1997-07-08 Akzo Nobel N.V. Method and instrument for automatically performing analysis relating to thrombosis and hemostasis
AU1414292A (en) * 1991-02-15 1992-09-15 Formulogics, Inc. Determination of contaminants
US5580744A (en) * 1992-04-27 1996-12-03 Avocet Medical, Inc. Test article and method for performing blood coagulation assays
US5344036A (en) * 1992-06-04 1994-09-06 Akzo N.V. Container system
US5447838A (en) * 1992-08-05 1995-09-05 Hybritech Incorporated Protein-dye conjugate for confirmation of correct dilution of calibrators
US5919707A (en) * 1994-12-22 1999-07-06 Nalco Chemical Company Monitoring of rolling oil emulsions
US6521182B1 (en) * 1998-07-20 2003-02-18 Lifescan, Inc. Fluidic device for medical diagnostics
US6830934B1 (en) * 1999-06-15 2004-12-14 Lifescan, Inc. Microdroplet dispensing for a medical diagnostic device
DE10257716B4 (de) 2002-12-11 2005-12-29 Institut für Textilchemie der Deutschen Institute für Textil- und Faserforschung Stuttgart Optischer Sensor zur Bestimmung von Farbstoffkonzentrationen in flüssigen Medien und Verfahren zu dessen Betrieb
US7283245B2 (en) * 2004-01-20 2007-10-16 General Electric Company Handheld device with a disposable element for chemical analysis of multiple analytes
US20060001551A1 (en) * 2004-06-30 2006-01-05 Ulrich Kraft Analyte monitoring system with wireless alarm
US20060000709A1 (en) * 2004-06-30 2006-01-05 Sebastian Bohm Methods for modulation of flow in a flow pathway
US20060002817A1 (en) * 2004-06-30 2006-01-05 Sebastian Bohm Flow modulation devices
US20060000710A1 (en) 2004-06-30 2006-01-05 Klaus Peter Weidenhaupt Fluid handling methods
CA2719012C (en) 2008-03-21 2013-07-09 Abbott Point Of Care, Inc. Method and apparatus for detecting and counting platelets individually and in aggregate clumps
EP2265946B1 (en) 2008-03-21 2012-08-01 Abbott Point Of Care, Inc. Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
JP5539309B2 (ja) 2008-03-21 2014-07-02 アボット ポイント オブ ケア インコーポレイテッド 赤血球中に含まれるヘモグロビンの固有色素を利用して血液試料の赤血球指数を決定するための方法及び装置
EP3109621A1 (en) 2008-03-21 2016-12-28 Abbott Point Of Care, Inc. Apparatus for analyzing individual cells or particulates in a blood sample based on fluorescence and optical density images of the sample
JP4887464B2 (ja) * 2008-03-21 2012-02-29 アボット ポイント オブ ケア インコーポレイテッド 生体試料を撮像するように構成された撮像機器の焦点位置を決定するための方法及び装置
ES2416404T3 (es) * 2008-04-02 2013-07-31 Abbott Point Of Care, Inc. Disolución en gradiente de auto-calibración en un ensayo de constituyentes y aparato de disolución en gradiente realizado en una muestra de película delgada
CA2720077C (en) * 2008-04-09 2014-08-05 Abbott Point Of Care, Inc. Method for measuring the area of a sample disposed within an analysis chamber
WO2009126505A1 (en) * 2008-04-09 2009-10-15 Abbott Point Of Care, Inc. Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber
US20100255605A1 (en) * 2009-04-02 2010-10-07 Abbott Point Of Care, Inc. Method and device for transferring biologic fluid samples
CN103154735B (zh) * 2009-05-11 2018-10-02 连接Dx股份有限公司 用于检测分析物的方法和组合物
EP2519820B1 (en) * 2009-12-31 2013-11-06 Abbott Point Of Care, Inc. Method and apparatus for determining mean cell volume of red blood cells
WO2011116305A1 (en) 2010-03-18 2011-09-22 Abbott Point Of Care, Inc. Method and apparatus for optically determining at least one hemoglobin related parameter of a whole blood sample
CN102128796B (zh) * 2010-12-07 2012-07-25 桂林理工大学 一种测定硫酸庆大霉素的方法
US9476871B2 (en) * 2012-05-02 2016-10-25 Diatech Oncology Llc System and method for automated determination of the relative effectiveness of anti-cancer drug candidates

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3059524A (en) * 1958-06-04 1962-10-23 Grassmann Wolfgang Method and apparatus for the continuous colorimetric determination of the individualcomponents of a mixture
US4204839A (en) * 1978-08-09 1980-05-27 Eastman Kodak Company Fluorimetric analysis method for bilirubin
US4274744A (en) * 1978-11-21 1981-06-23 Medico Electronic, Inc. Direct reading digital colorimeter
US4329149A (en) * 1980-03-06 1982-05-11 Hach Chemical Company Method for spectrophotometric compensation for colorimetric reagent variation
DE3019612A1 (de) * 1980-05-22 1981-11-26 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes thrombinpraeparat
JPS5832168A (ja) * 1981-08-19 1983-02-25 Olympus Optical Co Ltd 分析装置
US4506626A (en) * 1981-11-02 1985-03-26 Schurman Richard H Apparatus for controlling the proportions of a fluid
US4543335A (en) * 1982-12-20 1985-09-24 Miles Laboratories, Inc. Device and method for the quantitative determination of heparin in mammalian blood plasma
US4849340A (en) * 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
US4911549A (en) * 1988-04-05 1990-03-27 Baxter International Inc. Heparin monitoring system and methodology

Also Published As

Publication number Publication date
FI922478A0 (fi) 1992-05-29
WO1991008461A1 (en) 1991-06-13
EP0502983A1 (en) 1992-09-16
AU655577B2 (en) 1995-01-05
IE904276A1 (en) 1991-06-05
JPH05502723A (ja) 1993-05-13
EP0502983A4 (en) 1993-05-05
DE69028312D1 (de) 1996-10-02
CA2069887A1 (en) 1991-06-02
KR100210736B1 (ko) 1999-07-15
DE69028312T2 (de) 1997-01-16
GR3021261T3 (en) 1997-01-31
AU1628795A (en) 1995-06-29
DK0502983T3 (ko) 1997-01-20
AU6951291A (en) 1991-06-26
IE75698B1 (en) 1997-09-10
US5068181A (en) 1991-11-26
JP2902108B2 (ja) 1999-06-07
FI101575B (fi) 1998-07-15
ATE142018T1 (de) 1996-09-15
EP0502983B1 (en) 1996-08-28
FI101575B1 (fi) 1998-07-15
ES2095310T3 (es) 1997-02-16
CA2069887C (en) 2004-02-10
FI922478A (fi) 1992-05-29

Similar Documents

Publication Publication Date Title
KR920704118A (ko) 스캐닝 분광 광도계에서 시약 전달을 모니터하는 방법
Miller Recent developments in fluorescence and chemiluminescence analysis. Plenary lecture
Sackett et al. Hydrophobic surfaces of tubulin probed by time-resolved and steady-state fluorescence of nile red.
KR920704126A (ko) 형광 변화의 모니터에 의한 색반응의 측정 방법
ATE173820T1 (de) Verfahren zur erfassung von biomolekülen, toxischen substanzen, polymeren und pharmazeutischen wirkstoffen mittels zeitaufgelöster laserspektroskopie
Kessler et al. Laser-induced fluorometric determination of albumin using longwave absorbing molecular probes
US4252783A (en) Reducing fluorescent background in fluorescent immunoassays
ATE142622T1 (de) Elektrochemilumineszente rhenium-fraktionen und verfahren
McGown et al. Phase-resolved fluorescence in chemical analysis
Chang et al. Determination of magnesium and zinc in human brain tissue by atomic absorption spectroscopy
Quirin et al. Self-referencing fluorescence sensor for monitoring conversion of nonisothermal polymerization and nanoscale mixing of resin components
EP0027917B1 (de) Reagenz und Verfahren zur Bestimmung von Hydrazin
KR920704117A (ko) 피브리노겐 직접 분석 방법
Huang et al. Binding equilibrium study of phosphotungstic acid and HSA or BSA with UV spectrum, fluorescence spectrum and equilibrium dialysis
Zurawsky et al. Assessment of dielectric enrichment around two fluorophores in binary solvents
Roberts et al. Age‐related changes in the human lens as monitored by detection of porphyrin excited states
Clyne et al. Studies of BrCl by laser-induced fluorescence. Part 4.—Lower levels (v′⩽ 3) of the B 3 Π (0+) manifold
Davidson et al. Effect of variation of temperature on second-derivative fluorescence spectra. Part I. Spectral changes and uncorrected temperature coefficients
KR850007485A (ko) 개선된 형광분석법
KR20010092484A (ko) 혈청내 에토석시아미드의 농도 측정 방법
Villari et al. Time-Resolved Fluorescence: An Approach in Protein Analysis
Kern p-Aminobenzoic Acid
KR20010092483A (ko) 혈청내 발프로익산의 농도 측정 방법
KR20010092485A (ko) 혈청내 데오필린의 농도 측정 방법

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19920530

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 19951201

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19920530

Comment text: Patent Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19980709

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 19990129

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 19990428

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 19990429

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20020418

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20030417

Start annual number: 5

End annual number: 5

FPAY Annual fee payment

Payment date: 20040422

Year of fee payment: 6

PR1001 Payment of annual fee

Payment date: 20040422

Start annual number: 6

End annual number: 6

LAPS Lapse due to unpaid annual fee
PC1903 Unpaid annual fee

Termination category: Default of registration fee

Termination date: 20060410