KR900700883A - 식육의 질을 평가하기 위한 스카톨의 검출방법 - Google Patents
식육의 질을 평가하기 위한 스카톨의 검출방법Info
- Publication number
- KR900700883A KR900700883A KR1019890701604A KR890701604A KR900700883A KR 900700883 A KR900700883 A KR 900700883A KR 1019890701604 A KR1019890701604 A KR 1019890701604A KR 890701604 A KR890701604 A KR 890701604A KR 900700883 A KR900700883 A KR 900700883A
- Authority
- KR
- South Korea
- Prior art keywords
- reaction mixture
- scatol
- antibody
- sample
- solid substrate
- Prior art date
Links
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Natural products C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 title claims 16
- 238000001514 detection method Methods 0.000 title 1
- 235000013372 meat Nutrition 0.000 title 1
- 239000011541 reaction mixture Substances 0.000 claims 25
- 239000000758 substrate Substances 0.000 claims 17
- 238000000034 method Methods 0.000 claims 14
- 239000007787 solid Substances 0.000 claims 14
- 238000011109 contamination Methods 0.000 claims 8
- 102000004190 Enzymes Human genes 0.000 claims 7
- 108090000790 Enzymes Proteins 0.000 claims 7
- 210000004369 blood Anatomy 0.000 claims 7
- 239000008280 blood Substances 0.000 claims 7
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical group C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims 6
- 235000015277 pork Nutrition 0.000 claims 6
- 241000282887 Suidae Species 0.000 claims 5
- 239000011535 reaction buffer Substances 0.000 claims 4
- 238000005406 washing Methods 0.000 claims 4
- 230000003287 optical effect Effects 0.000 claims 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 claims 1
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 claims 1
- 102000004157 Hydrolases Human genes 0.000 claims 1
- 108090000604 Hydrolases Proteins 0.000 claims 1
- 102000004316 Oxidoreductases Human genes 0.000 claims 1
- 108090000854 Oxidoreductases Proteins 0.000 claims 1
- 239000012267 brine Substances 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 210000002381 plasma Anatomy 0.000 claims 1
- 229940074386 skatole Drugs 0.000 claims 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims 1
- 239000003656 tris buffered saline Substances 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/963—Methods of stopping an enzyme reaction or stabilizing the test materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/809—Multifield plates or multicontainer arrays
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/81—Tube, bottle, or dipstick
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/822—Identified hapten
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
내용없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (21)
- (a) 반응버퍼에서 돼지시료와 항-스카톨 항체를 합쳐 반응혼합물을 형성하고; (b) 이 반응 혼합물을, 반응 혼합물에 존재하는 스카톨을 가지는 항체-스카톨 착물이 형성되기가 충분한 시간동안 배양하고; 그리고 (c) 돈육중의 숫돼지 오염 지표로서 항체-스타톨의 양을 측정하는 것으로 이루어지는, 돈육중의 숫돼지 오염을 측정하기 위한 방법.
- 제1항에 있어서, 시료가 전혈, 혈청 또는프라즈마로 이루어짐을 특징으로 하는 방법.
- 제1항에 있어서, 시료가 등 지방추출물로 이루어짐을 특징으로 하는 방법.
- 제1항에 있어서, 항체가 고체기질에 부착됨을 특징으로 하는 방법.
- 제4항에 있어서, 아는 양의 라벨된 스카톨 동족물을 시료와 함께 반응 혼합물을 가하고 고체 기질에 결합된 라벨의 양을 검출함으로써 측정함을 특징으로 하는 방법.
- 제1항에 있어서, 시료중에 존재하는 스카톨을 가지는 항체-스카톨 착물이 형성되기에 충분한 시간동안 시료를 아는양의 항-스카톨 항체와 반응시켜 반응 혼합물을 형성시키고, 반응 혼합물을 고체기질-부착된 스카톨 동족물과 함께 배양하고 기질에 결합되었거나 반응 혼합물중에 있는 항체의 양을 측정함을 특징으로 하는 방법.
- (a) 반응 버퍼에서 돼지의 혈액시료와 고체기질의 부착된 항-스카톨 항체를 합쳐 반응 혼합물을 형성하고; (b) 이 반응 혼합물을, 반응 혼합물에 존재하는 스카톨을 가지는 항체-스카톨 착물이 형성되기가 충분한 시간동안 배양하고; (c) 고체기질을 씻고; 그리고 (d) 돈육중의 숫퇘지 오염지표로서 고체기질에 부착된항체-스타톨 착물의 양을 측정하는 것으로 이루어지는, 돈육중의 숫돼지 오염을 검출하기 위한 방법.
- (a) 반응 버퍼에서, 라벨돈 스카톨 동족물, 돼지의 혈액시료 및 고체기질에 부착돈 항-스타톨 항체를 합쳐 반응 혼합물을 형성하고; (b) 이 반응 혼합물을, 반응 혼합물에 존재하는 스카톨을 가지는 항체-스타톨 착물이 형성되기에 충분한 시간동안 배양하고; (c) 고체기질을 씻고; 그리고 (d) 돈육중의 숫퇘지 오염의 지표로서 아는 양의 스카톨을 가지는 시료와 비교하여 고체기질에 결합된 라벨의 양을 측정하는 것으로 이루어지는, 돈육중의 숫돼지 오염을 검출하기 위한 방법.
- 제8항에 있어서, 라벨이 효소임을 특징으로 하는 방법.
- 제9항에 있어서, 효소가 가수분해 효소임을 특징으로 하는 방법.
- 제9항에 있어서, 효소가 산화환원효소임을 특징으로 하는 방법.
- 제8항에 있어서, 스카톨 동족물이 인돌-3-브티르산, 3-카르복시메틸인돌 또는 3-메틸인돌임을 특징으로 하는 방법.
- 제12항에 있어서, 접합물이 인돌-3-브티르산/알칼리성 포스파타아제 접합물임을 특징으로 하는 방법.
- 제8항에 있어서, 측정단계(d)가 (i) 효소기질을 고체기질에 가하여 제2의 반응 혼합물을 형성하고; (ⅱ) 이 제2반응 혼합물을, 검출성 기질숙성 생성물이 형성되기에 충분한 시간동안 배양하고; (ⅲ) 제2반응 혼합물의 강학적 밀도를 측정하는 것으로 이루어짐을 특징으로 하는 방법.
- (a) 반응 버퍼에서 스카톨 동족물/효소접합물, 돼지의 혈액시료 및 고체기질에 부착된 항-스타톨 항체를 합쳐 제1의 반응 혼합물을 형성하고; (b) 제1반응 혼합물을, 항체-스카톨 착물이 형성되기에 충분한 시간동안 배양하고; (c) 고체기질을 세척버퍼로 세척하고; (d) 효소기질 용액을 고체기질과 함께 배양하여 제2의 반응 혼합물을 형성하고; 그리고 (e) 돈육중의 숫돼지 오염의 지표로서 아는 양의 스카톨을 가지는 시료의 광학적 밀도와 비교하여 제2반응 혼합물의 밀도를 측정하는 것으로 이루어지는, 돈육중의 숫돼지 오염을 검출하기 위한 방법.
- (a) 돼지의 혈액시료, 트리스-버퍼화된 식염수중의 인돌-3-부티르산/알칼리성 포스파타아제 접합물, 및 마이크로 타이터 플레이트에 부착된 항-스카톨 항체를 합쳐 제1반응 혼합물을 형성하고; (b) 이 반응 혼합물을 약 4℃에서 약 4시간동안 배양하고; (c) 고체기질을 식염수-포릴옥실렌솔비탄 버퍼로 세척하고; (d) 약 37℃에서 약 1시간동안 마이크로타이터 플레이트로 P-니트로페닐포스페이트용액을 배양하고; (e) 효소활성을 중지시키기 위해 제2반응 혼합물을 pH를 변경시켜 제3의 반응혼합물을 형성하고; 그리고 (f) 시료의 스카톨 농도를 측정하기 위해 아느 양의 스카톨을 가지는 시료의 광학적밀도와 비교하여 제3반응 혼합물의 광학적 밀도를 측정하여, 시료가 약 0.3ppm이상의 스카톨을 가질 때 돈육을 숫돼지 오염된 것으로 판정하는 것으로 이루어지는, 돈육중의 숫돼지 오염을 검출하기 위한 방법.
- 제16항에 있어서, 돼지의 혈액시료가 혈청 또는 플라즈마로 이루어짐을 특징으로 하는 방법.
- 분리된 용기에 항-스카톨 항체와 라벨된 스카톨 동족물을 포함하는, 돼지 혈액시료중의 숫돼지 오염을 측정하기 취한 킷.
- 제18항에 있어서, 항-스카톨 항체가 마이크로타이터 플레이트에 부착됨을 특징으로 하는 킷.
- 제18항에 있어서, 스카톨 동족물이 효소로 라벨됨을 특징으로 하는 킷.
- 제19항에 있어서, 분리된 용기에 스카톨을 부가적으로 포함하는 킷.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/138,305 US4906563A (en) | 1987-12-28 | 1987-12-28 | Detection of skatole for meat quality |
| US138,305 | 1987-12-28 | ||
| PCT/US1988/004428 WO1989006361A1 (en) | 1987-12-28 | 1988-12-12 | Detection of skatole for meat quality |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR900700883A true KR900700883A (ko) | 1990-08-17 |
Family
ID=22481434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019890701604A KR900700883A (ko) | 1987-12-28 | 1988-12-12 | 식육의 질을 평가하기 위한 스카톨의 검출방법 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4906563A (ko) |
| EP (1) | EP0368942A4 (ko) |
| KR (1) | KR900700883A (ko) |
| DK (1) | DK422189A (ko) |
| PL (1) | PL276786A1 (ko) |
| WO (1) | WO1989006361A1 (ko) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK200890D0 (da) * | 1990-08-22 | 1990-08-22 | Ulf Nonboe | Metode til bestemmelse af lugtende svinekoed |
| US5372822A (en) * | 1994-03-07 | 1994-12-13 | Fahim; Mostafa S. | Chemical castration |
| US5705749A (en) * | 1995-09-25 | 1998-01-06 | Biostar Inc. | Use of ultrasonography to evaluate size and echotexture of gonadal and accessory genital structures in domestic animals |
| AU725973B2 (en) * | 1997-03-14 | 2000-10-26 | University Of Guelph | Method for determining predisposition to boar taint |
| US20050019788A1 (en) * | 1998-04-08 | 2005-01-27 | University Of Guelph | Genetic markers for skatole metabolism |
| US20040033552A1 (en) * | 1998-04-08 | 2004-02-19 | Squires E. James | Method of detecting and reducing boar taint |
| EP0997125A1 (en) * | 1998-10-29 | 2000-05-03 | The Procter & Gamble Company | Measuring system and measuring sensor for detecting and measuring the presence of faeces in an absorbent article |
| WO2001023601A2 (en) * | 1999-09-30 | 2001-04-05 | University Of Guelph | Novel enzymes and metabolites involved in skatole metabolism |
| US7202035B2 (en) * | 1999-09-30 | 2007-04-10 | University Of Guelph | Genetic markers for skatole metabolism |
| US20060024708A1 (en) * | 2004-06-17 | 2006-02-02 | University Of Guelph | Porcine sulfotransferase 2A1 polynucleotide sequence, protein, and methods of use for same |
| US7905154B2 (en) * | 2004-11-29 | 2011-03-15 | Jones Jr Arthur T | Apparatus and method of contaminant detection for food industry |
| US8627816B2 (en) | 2011-02-28 | 2014-01-14 | Intelliject, Inc. | Medicament delivery device for administration of opioid antagonists including formulations for naloxone |
| US8939943B2 (en) | 2011-01-26 | 2015-01-27 | Kaleo, Inc. | Medicament delivery device for administration of opioid antagonists including formulations for naloxone |
| US9517307B2 (en) | 2014-07-18 | 2016-12-13 | Kaleo, Inc. | Devices and methods for delivering opioid antagonists including formulations for naloxone |
| WO2017147709A1 (en) * | 2016-03-03 | 2017-09-08 | Phytronix Technologies Inc. | Method for detecting boar taint |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6160648A (ja) * | 1984-08-31 | 1986-03-28 | Teikoku Hormone Mfg Co Ltd | ２−（３，５−ジアルキル−４−ヒドロキシフエニル）インド−ル誘導体 |
| US4610877A (en) * | 1984-10-19 | 1986-09-09 | Research Corporation | Immunological prevention of boar odor in uncastrated male pigs |
-
1987
- 1987-12-28 US US07/138,305 patent/US4906563A/en not_active Expired - Fee Related
-
1988
- 1988-12-12 WO PCT/US1988/004428 patent/WO1989006361A1/en not_active Application Discontinuation
- 1988-12-12 EP EP19890901240 patent/EP0368942A4/en not_active Withdrawn
- 1988-12-12 KR KR1019890701604A patent/KR900700883A/ko not_active Application Discontinuation
- 1988-12-28 PL PL27678688A patent/PL276786A1/xx unknown
-
1989
- 1989-08-28 DK DK422189A patent/DK422189A/da not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0368942A4 (en) | 1991-06-19 |
| EP0368942A1 (en) | 1990-05-23 |
| DK422189D0 (da) | 1989-08-28 |
| US4906563A (en) | 1990-03-06 |
| PL276786A1 (en) | 1989-08-07 |
| DK422189A (da) | 1989-08-28 |
| WO1989006361A1 (en) | 1989-07-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 19890824 |
|
| PG1501 | Laying open of application | ||
| PC1203 | Withdrawal of no request for examination | ||
| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |