[go: up one dir, main page]

KR830006307A - 디아세틸 세팔로스포린의 새로운 제조방법 - Google Patents

디아세틸 세팔로스포린의 새로운 제조방법 Download PDF

Info

Publication number
KR830006307A
KR830006307A KR1019810002646A KR810002646A KR830006307A KR 830006307 A KR830006307 A KR 830006307A KR 1019810002646 A KR1019810002646 A KR 1019810002646A KR 810002646 A KR810002646 A KR 810002646A KR 830006307 A KR830006307 A KR 830006307A
Authority
KR
South Korea
Prior art keywords
cephalosporin
diacetylcephalosporin
hydrolyzable
acetyl esterase
strain
Prior art date
Application number
KR1019810002646A
Other languages
English (en)
Other versions
KR840002294B1 (ko
Inventor
히로시 이마나까
도시오 미요시
토시오 코모노미
요시아끼 쿠보치
세이지로오 하토오리
다께시 카와기다
Original Assignee
후지사와 도모 키찌로오
후지사와 야구힝 고오교 가부시기가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 후지사와 도모 키찌로오, 후지사와 야구힝 고오교 가부시기가이샤 filed Critical 후지사와 도모 키찌로오
Publication of KR830006307A publication Critical patent/KR830006307A/ko
Application granted granted Critical
Publication of KR840002294B1 publication Critical patent/KR840002294B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/06—Cephalosporin C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

내용 없음

Description

디아세틸 세팔로스포린의 새로운 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 pH와 상대적 효소활성의 관계를 나타낸 그래프.
제2도는 pH와 상대적 효소활성의 관계를 나타낸 그래프.
제3도는 온도와 상대적효소활성의 관계를 나타낸 그래프.
제4도는 온도와 상대적효소활성의 관계를 나타낸 그래프.
제5도는 온도와 상대적 효소활성의 관계를 나타낸 그래프이다.

Claims (8)

  1. 오레오바시듐(Aureobasidium)속 또는 그 돌연변이체에 속하는 균주에 의해 생성된 아세틸에스 테라제와 세팔로스포린 C를 접촉시키는 것을 특징으로 하는 효소가수분해에 의하여 세팔로스포린 C로 부터 디아세틸세팔로스포린 C를 제조하는 방법.
  2. 균주가 오레오바시듐 풀루란인 청구범위 1에 따른 방법.
  3. 균주가 오레오바시듐풀루란 IFO 4466인 청구범위 1에 따른 방법.
  4. 세팔로스포린 C가 오레오바시듐속의 배양브로스에 함유된 아세틸에스 테라제와 접촉되는 청구범위 1에 따른 방법.
  5. 오레오바시듐속의 균주로 배양된 브로스로부터 얻어진 처리물지렝 함유된 아세틸에스테라제와 세팔로스포린을 접촉시키는 청구범위 1에 따른 방법.
  6. 처리된 물질이 균사체, 비가동 균사체, 비가동효소와 정제된 효소제제로부터 선정되는 청구범위 6에 따른 방법.
  7. 세팔로스포린 C가 디아세틸세팔로스포린 C를 제조하기 위해서 배양된 브로스에서 생성된 아세틸에스테라제에 의해서 가수분해될 때까지 세팔로스포린 C와 아세틸에스 테라제의 접촉이 세팔로스포린 C-생성미생물의 혼합 배양에 의해 실시되는 청구범위 1에 따른 방법.
  8. (a) 세팔로스포린 C를 디아세틸세팔로스포린 C로 가수분해하는 능력
    (b) 썹스트레이트 특이성
    가수분해성 : 초산에스테르
    비가수분해성 : 초산에스테르의 에스테르
    (c) 최적 pH : pH4이하
    (d) 안정한 pH범위 : pH4.0-6.0
    (e) 최적온도 : 약 37℃
    (f) 안정온로 범위 :60℃이하
    (g) km값 : 11.5mM와
    (h) 금속이온 효과 : 효소작용이 Cu2+,Mn2+,Mg2+,Zn2+,Ni2+,Ca2+,Ba2+와 Fe2+로부터 선정된 금속이온에 의해서 영향을 받지 않음
    상기의 성질의 갖는 아세틸에스테라제 4466-1(단, 썹트레이트는 상기(c)-(h)에 있는 세팔로스포린 C임)과
    (a) 세팔로스포린 C를 디아세틸세팔로스포린 C로 가수분해하는 능력
    (b) 썹스트레이트 특이성
    가수분해성 : 초산에스테르
    비가수분해성 : 초산에스테르의 에스테르
    (c) 최적의 pH : pH4이하
    (d) 안정 pH : 4.0-6.0
    (e) 최적 온도 : 약 37℃
    (f) km값 : 13.7mM과
    (h) 금속이온의 효과 : 효소작용이 Cu2+,Mn2+,Zn2+,Ni2+,Ca2+,Ba2+Ba2+와 Fe3+로부터 선정된 금속이온에 의하여 영향을 받지 않음.
    상기의 성질을 갖는 아세틸에스테라제 4466-Ⅱ(단, 썹스트레이트는 (c)-(h)에 있는 세팔로스포린 C이다)로 부터 선정되는 아세틸에스테라제.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019810002646A 1980-07-21 1981-07-21 디아세틸세팔로스포린 c의 제조방법 KR840002294B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8023834 1980-07-21
GB8023834 1980-07-21

Publications (2)

Publication Number Publication Date
KR830006307A true KR830006307A (ko) 1983-09-20
KR840002294B1 KR840002294B1 (ko) 1984-12-15

Family

ID=10514925

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019810002646A KR840002294B1 (ko) 1980-07-21 1981-07-21 디아세틸세팔로스포린 c의 제조방법

Country Status (7)

Country Link
US (2) US4414328A (ko)
EP (1) EP0044736B1 (ko)
JP (2) JPS5739798A (ko)
KR (1) KR840002294B1 (ko)
AT (1) ATE12950T1 (ko)
DE (1) DE3170118D1 (ko)
ES (1) ES504166A0 (ko)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4847200A (en) * 1983-11-25 1989-07-11 Queen's University At Kingston Biosynthesis of unnatural cephalosporins
DE3620801A1 (de) * 1986-06-20 1988-02-25 Pfaff Ind Masch Naehmaschine mit einer werkstueck-ausrichtvorrichtung
GB8624686D0 (en) * 1986-10-15 1986-11-19 Erba Farmitalia Preparing penems
US5260214A (en) * 1988-07-19 1993-11-09 Takara Shuzo Co. Aureobasidium pullulans which produces antibiotic R106
US5057493A (en) * 1988-07-19 1991-10-15 Takara Shuzo Co., Ltd. Novel antibiotics r106
US5082772A (en) * 1988-10-24 1992-01-21 Eli Lilly And Company Process for preparing deacetylcephalosporin c
JP2528888Y2 (ja) * 1990-09-11 1997-03-12 ティーディーケイ株式会社 Ａｃインレットフィルタコネクタ
IT1252308B (it) * 1990-12-21 1995-06-08 Antibioticos Spa Procedimento enzimatico per la produzione di acido 7- amminocefalosporanico e derivati
US5512454A (en) * 1994-02-03 1996-04-30 Bristol-Myers Squibb Company Enzymatic acylation of 3-hydroxymethyl cephalosporins
US8198434B2 (en) 2008-05-07 2012-06-12 Idexx Laboratories, Inc. Process for preparing cefsulodin sodium
JP5698789B2 (ja) * 2013-04-18 2015-04-08 ファナック株式会社 ワークを搬送するロボットの制御装置
CN107915749A (zh) * 2016-10-11 2018-04-17 伊犁川宁生物技术有限公司 一种纯化头孢菌素c发酵液的方法
CN111850078A (zh) * 2020-07-21 2020-10-30 伊犁川宁生物技术有限公司 一种头孢菌素c的发酵方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH475284A (de) * 1963-11-19 1969-07-15 Ciba Geigy Verfahren zur Herstellung von Desacetyl-7-amino-cephalosporansäure
GB1121308A (en) 1964-10-30 1968-07-24 Glaxo Lab Ltd Cephalosporins
JPS4948760B1 (ko) * 1970-12-25 1974-12-23
GB1474519A (ko) 1973-05-14 1977-05-25
US3976546A (en) * 1974-11-08 1976-08-24 Glaxo Laboratories Limited Cephalosporins
GB1531212A (en) * 1974-11-08 1978-11-08 Glaxo Lab Ltd Enzymatic hydrolysis of 3-acyloxymethylceph-3-em-4-carboxylic acids or salts thereof

Also Published As

Publication number Publication date
JPS5739798A (en) 1982-03-05
ES8302098A1 (es) 1983-01-01
EP0044736A3 (en) 1982-08-18
EP0044736A2 (en) 1982-01-27
US4414328A (en) 1983-11-08
EP0044736B1 (en) 1985-04-24
JPH0147158B2 (ko) 1989-10-12
DE3170118D1 (en) 1985-05-30
KR840002294B1 (ko) 1984-12-15
US4517299A (en) 1985-05-14
ATE12950T1 (de) 1985-05-15
JPH0249580A (ja) 1990-02-19
ES504166A0 (es) 1983-01-01
JPH0251594B2 (ko) 1990-11-07

Similar Documents

Publication Publication Date Title
Iizumi et al. Purification and characterization of a thermostable lipase from newly isolated Pseudomonas sp. KWI-56
US4444886A (en) Diacetinase from Bacillus subtilis
KR830006307A (ko) 디아세틸 세팔로스포린의 새로운 제조방법
ES8602116A1 (es) Un procedimiento para hidrolizar almidon con una enzima de alfa-amilasa
Yamaguchi et al. Production and properties of lipase from a newly isolated Chromobacterium
AU615661B2 (en) Acid urease and production thereof
DE3781192T2 (de) Mikroorganismus, esterase und verfahren zu deren herstellung.
JPS557001A (en) Preparation of n-carbamoyl-d-thienylglycine
Harada Preparation of Aspergillus oryzae Enzymes1
Horikoshi et al. Production of 1, 3-β-glucanase by Bacillus no. 221: An alkalophilic microorganism
Sunaga et al. Production of penicillinase by an alkalophilic Bacillus
CA1153327A (en) Process for the preparation of penicillin derivatives
DE3161317D1 (en) Process for the production of glucose dehydrogenase and microorganisms suitable therefor
DE3062041D1 (en) Process for the production of cholesterol esterase
KR880003009A (ko) 카르니틴의 제조방법
SU1650697A1 (ru) Штамм бактерий BacILLUS LIснеNI FоRмIS - продуцент рестриктазы В @ 13I
US3847744A (en) Method of producing elastases by bacteria
JPS5642587A (en) Production of chlesterine esterase
JPH0789913B2 (ja) リパ−ゼａｍｌの製造法
JPH0574353B2 (ko)
Nakagawa et al. Properties of the extracellular lytic proteinase of Pseudomonas sp.
JPH07227276A (ja) リパーゼ、これを産生する微生物及び該微生物の取得方法
JPH0127714B2 (ko)
INAI et al. Studies on the Electrophoresis of Marine Bacterial Enzymes-II Esterase, Malate Dehydrogenase, and Lactate Dehydrogenase
JPH0147150B2 (ko)

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19810721

PG1501 Laying open of application
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19840724

Patent event code: PE09021S01D

PG1605 Publication of application before grant of patent

Comment text: Decision on Publication of Application

Patent event code: PG16051S01I

Patent event date: 19841124

PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 19850228

PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 19850313

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 19850313

End annual number: 3

Start annual number: 1

PR1001 Payment of annual fee

Payment date: 19871112

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 19881210

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 19891127

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 19901203

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 19911204

Start annual number: 8

End annual number: 8

PR1001 Payment of annual fee

Payment date: 19921205

Start annual number: 9

End annual number: 9

PR1001 Payment of annual fee

Payment date: 19931207

Start annual number: 10

End annual number: 10

PR1001 Payment of annual fee

Payment date: 19941212

Start annual number: 11

End annual number: 11

PR1001 Payment of annual fee

Payment date: 19951212

Start annual number: 12

End annual number: 12

PC1903 Unpaid annual fee