KR102759009B1 - Method for manufacturing feed additives containing metabolites derived from microorganisms and feed additives prepared thereof - Google Patents

Abstract

The present invention provides a method for producing a feed additive comprising a liquid microbial culture solution separated by centrifuging a microbial culture solution, at least one vitamin selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E, a sweetener, a mineral, an organic acid, and at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate, and sodium selenite, and a feed additive produced thereby.

Description

Method for manufacturing feed additives containing microbial metabolites and feed additives prepared therefrom {METHOD FOR MANUFACTURING FEED ADDITIVES CONTAINING METABOLITES DERIVED FROM MICROORGANISMS AND FEED ADDITIVES PREPARED THEREOF}

The present invention relates to a method for producing a feed additive including a microbial metabolite, and a feed additive produced thereby. More specifically, the present invention relates to a method for producing a feed additive including a microbial metabolite, which uses a medium discarded after culturing a Streptomyces sp. microorganism, a Bacillus subtilis sp. microorganism, or a Lactobacillus plantarum sp. microorganism, to increase the efficiency of digestion and absorption of livestock feed, and to a feed additive produced thereby, which has the effect of suppressing bacteria causing intestinal odor.

In general, feed is a substance that provides organic or inorganic nutrients necessary to sustain the life of livestock and produce products such as eggs, milk, and meat. Good feed should have a high nutrient content, be free of harmful ingredients to livestock products, produce a high yield, be easily available, fresh, and have a high digestibility.

The supply and demand status of domestic livestock feed is mostly mixed feed using imported grains as the main raw material, and low-temperature fermented silage using self-supplied raw materials by farms is used as supplementary feed. Conventional grain mixed feed is sold as dry feed mixed/packaged with protein (CP), total digestible nutrients (TDN), crude fiber, ash, calcium (Ca), phosphorus (P), and vitamin Z in the composition ratio of the feed menu, or mixed/packaged mixed feed mixed with grains and hay.

Supplementary feed refers to feed additives that are added to compound feed to improve the functionality of livestock products, such as digestibility, physiological activity, growth promotion, and immune enhancement. The livestock industry commonly uses antibiotics to promote the growth of livestock and prevent or treat livestock diseases (Guo, F. C. et al., 2004, Effect of a Chinese herb medicine formulation, as an alternative for antibiotics, on performance of broilers, Br Poult Sci., 45, 793-797). However, since frequent use of antibiotics can increase resistant microorganisms, reducing the efficacy of the antibiotics or causing adverse effects on the human body by leaving them in livestock products, the use of antibiotics is gradually being regulated (Chae, M. H. et al., 2011, Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli isolated from food animals and raw meats in slaughterhouse in Korea during 2010, Kor. J. Vet. Publ. Hilth., 35, 239-245).

The physical, chemical and biological research on minerals, one of the five essential nutrients for humans and livestock, has recently been focused on livestock feed research. In the past, inorganic minerals were widely used as animal feed additives, but their utilization rate in the livestock body was only 20-30%, and the remainder was excreted as manure, which caused resource waste and environmental pollution problems. Therefore, chelated minerals that are bound to organic compounds such as proteins or carbohydrates have been used, and their absorption and utilization rate in the body has been reported to be quite high at 70-80% (Kratzer and Vohra, 1986), and various methods for producing such chelated minerals are known (US2877253, US5061815, US5504055, US6458981 B1, US7087775 B2, US7375243 B2, US2008/0248583 A1).

Accordingly, there is an increasing demand for feed additives that replace antibiotics, do not contain harmful ingredients to livestock products, and improve the functionality of livestock products, such as digestibility, physiological activity, growth promotion, and immunity enhancement.

Domestic registration patent No. 10-0372159

The problem to be solved by the present invention is to provide a method for producing a feed additive including a metabolite derived from a Streptomyces sp. microorganism, a Bacillus subtilis sp. microorganism, or a Lactobacillus plantarum sp. microorganism, thereby reducing the cost, manpower, and time for treating spent medium or spent culture fluid, and thereby improving the growth rate, reducing the mortality rate, enhancing the immune function, reducing the number of days to be shipped, and improving the meat quality grade and the meat quantity grade of livestock products, and a feed additive produced thereby.

In order to solve the above problem, the present invention comprises the steps of: obtaining a first culture solution by centrifuging a microbial culture solution of Streptomyces sp. and separating only the liquid culture solution; obtaining a second culture solution by centrifuging a microbial culture solution of Bacillus subtilis sp. and separating only the liquid culture solution; obtaining a third culture solution by centrifuging a microbial culture solution of Lactobacillus plantarum sp. and separating only the liquid culture solution; obtaining a first mixture by mixing at least one vitamin selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E with the first culture solution; obtaining a second mixture by mixing a sweetener with the second culture solution; and mixing a mineral and an organic acid with the third culture solution and at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate, and selenous acid. The present invention provides a method for producing a feed additive, including a step of mixing sodium to obtain a third mixture; and a step of mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction to produce a feed additive composition, and a feed additive produced thereby.

The method for producing a feed additive including a microbial metabolite according to the present invention and the feed additive produced thereby have the effects of reducing antibiotic resistance, containing no components harmful to livestock products, and improving the functionality of livestock products such as digestibility, physiological activity, growth promotion, and immune enhancement. In addition, the method has the effects of reducing the cost, manpower, and time for processing the medium or culture solution discarded after microbial culture, and can improve the growth rate of livestock products, reduce the mortality rate, enhance the immune function, reduce the number of days to be shipped, and improve the meat quality grade and meat quantity grade.

Hereinafter, various embodiments of the present invention will be described with reference to the attached drawings. It should be understood that the present invention is not limited to specific embodiments, but includes various modifications, equivalents, and/or alternatives of the embodiments of the present invention. In connection with the description of the drawings, similar reference numerals may be used for similar components.

In this document, the expressions "have", "can have", "include", or "may include" indicate the presence of a feature (e.g., a numerical value, function, operation, or component such as a part), but do not exclude the presence of additional features.

In this document, the expressions "A or B", "at least one of A and/or B", or "one or more of A or/and B" can include all possible combinations of the items listed together. For example, "A or B", "at least one of A and B", or "at least one of A or B" can all refer to cases where (1) at least one A is included, (2) at least one B is included, or (3) both at least one A and at least one B are included.

The expression "configured to" as used herein can be used interchangeably with, for example, "Suitable for", "Having the capacity to", "Designed to", "Adapted to", "Made to", or "Capable of". The term "configured to" does not necessarily mean "specifically designed to".

The terms used in this document are only used to describe specific embodiments and may not be intended to limit the scope of other embodiments. The singular expression may include the plural expression unless the context clearly indicates otherwise. The terms used herein, including technical or scientific terms, may have the same meaning as commonly understood by a person of ordinary skill in the art described in this document. Among the terms used in this document, terms defined in general dictionaries may be interpreted as having the same or similar meaning in the context of the related technology, and shall not be interpreted in an ideal or excessively formal meaning unless explicitly defined in this document. In some cases, even if a term is defined in this document, it cannot be interpreted to exclude the embodiments of this document.

The embodiments disclosed in this document are presented for the purpose of explanation and understanding of the disclosed technical contents, and do not limit the scope of the present invention. Therefore, the scope of this document should be interpreted to include all modifications or various other embodiments based on the technical idea of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. Prior to this, the terms or words used in this specification and claims should not be interpreted as limited to their usual or dictionary meanings, and should be interpreted as meanings and concepts that conform to the technical idea of the present invention based on the principle that the inventor can appropriately define the concept of the term in order to explain his or her own invention in the best way.

Therefore, the configurations of the embodiments described in this specification are only some of the most preferred embodiments of the present invention and do not represent all of the technical ideas of the present invention, and it should be understood that there may be various equivalents and modified examples that can replace them at the time of filing this application.

Throughout the specification, whenever a part is said to "include" a component, this does not mean that it excludes other components, but rather that it may include other components, unless otherwise stated.

Hereinafter, the present invention will be described in detail.

A method for manufacturing a feed additive according to one embodiment of the present invention

A step of obtaining a first culture solution by centrifuging a Streptomyces sp. microorganism culture solution and separating only the liquid culture solution;

A step of centrifuging a Bacillus subtilis sp. microbial culture to separate only the liquid culture solution and obtain a second culture solution;

A step of centrifuging a Lactobacillus plantarum sp. microbial culture solution and separating only the liquid culture solution to obtain a third culture solution;

A step of obtaining a first mixture by mixing at least one vitamin selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E into the first culture solution;

A step of obtaining a second mixture by mixing a sweetener into the second culture medium;

A step of mixing minerals and organic acids in the third culture solution and mixing sodium selenite with at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate to obtain a third mixture; and

It may include a step of mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction to prepare a feed additive composition.

Below, each step is explained in detail.

The step of centrifuging the above Streptomyces sp. microorganism culture solution and isolating only the liquid culture solution to obtain a first culture solution is to culture Streptomyces sp. microorganisms having antibacterial activity and isolate only the liquid culture solution containing metabolites of the Streptomyces sp. microorganisms.

The above Streptomyces sp. microorganisms produce various enzymes as well as secondary metabolites such as antibiotics such as antibacterial and antifungal agents, anticancer agents, and immunomodulators, and thus belong to an industrially important taxonomic group. In addition to producing geosmin, a volatile substance that causes an earthy odor, they also produce various secondary metabolites such as antibiotics and industrially useful enzymes. It is known as a very beneficial strain that produces more than half of the natural antibiotics (neomycin, streptomycin, tetracycline, chloramphenicol, daptomycin, lincomycin, etc.) used as pharmaceutical raw materials in hospitals.

The step of centrifuging the above Bacillus subtilis sp. microorganism culture solution and isolating only the liquid culture solution to obtain a second culture solution is to culture a Bacillus subtilis sp. microorganism having an immune function-enhancing effect and isolate only the liquid culture solution containing a metabolite of the Bacillus subtilis sp. microorganism.

The above Bacillus subtilis genus microorganism is a type of fermentation bacteria that grows on dry grass or straw, and is also called gochoryun. It is a heat-resistant and aerobic bacteria that forms endospores and can withstand extreme environments. Bacillus subtilis genus microorganisms are beneficial microorganisms that produce proteolytic enzymes as metabolites.

The step of centrifuging the above Lactobacillus plantarum sp. microorganism culture solution and isolating only the liquid culture solution to obtain a third culture solution is to culture Lactobacillus plantarum sp. microorganisms that inhibit the growth of harmful bacteria and isolate only the liquid culture solution containing the metabolites of the Lactobacillus plantarum sp. microorganisms.

The above Lactobacillus plantarum microorganisms can stimulate the secretion of mucin, which protects the intestinal wall, inhibits the growth of harmful bacteria, and adheres to the intestinal mucosa to prevent harmful substances such as bacteria from penetrating into the intestines, thereby enhancing immunity. In addition, it has a cholesterol-regulating function and can produce antifungal substances.

The step of obtaining the first mixture is to supplement the vitamin components lacking in the feed additive by mixing fat-soluble vitamins, vitamins A, D, and E, and water-soluble vitamins, vitamins B and C, into the first culture solution.

The step of obtaining the second mixture is to mix a sweetener into the second culture medium and add it to sweeten the feed. In addition to sweetening, the sweetener can have moisturizing and preservative effects and can also provide gloss to the feed, making it visually delicious. The sweetener added to the feed can be a natural sweetener such as thaumatin or stevia extract, or a synthetic sweetener such as saccharin or aspartame.

The step of obtaining the third mixture is to supplement minerals to the third culture medium, mix in an organic acid to adjust the pH of the mixture to 3 to 5, and mix in sodium selenite with at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate, thereby producing a feed additive that promotes the growth of livestock, improves disease resistance, and increases digestion efficiency.

The above minerals can be mixed into feed additives to strengthen immunity and regulate hormonal imbalances. By supplementing minerals to the third culture medium, a feed additive that can regulate acid-alkalinity and maintain hormonal balance can be manufactured. The above minerals are a type of metal mineral (e.g., Cu, Zn, Mn, Mo, Se, etc.) with divalent cations and are extracted from ores, so they are called minerals or inorganic substances. However, minerals exist in different forms in the bodies of plants and animals, and inorganic minerals are ingested by plants and animals and form chelate bonds with organic substances in the body, which are called chelated minerals. In the past, inorganic minerals were widely used as animal feed additives, but the utilization rate in the bodies of livestock is only 20-30%, and the remainder is excreted as manure, which has caused problems of resource waste and environmental pollution. Accordingly, chelated minerals that are chelated with organic compounds such as proteins or carbohydrates have been used, and their absorption and utilization in the body have been reported to be quite high at 70-80% (Kratzer and Vohra, 1986), and various methods for producing such chelated minerals are known. In the present invention, by mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction, the minerals can be used in a form in which the absorption and utilization in the body of livestock is increased.

The organic acid may include at least one selected from the group consisting of fumaric acid, formic acid, acetic acid, propionic acid, sorbic acid, citric acid, lactic acid, and butyric acid. The organic acid may lower the pH of the feed and the digestive organs. When the pH is maintained at a low level, pathogenic microorganisms that have entered the stomach of livestock together with the digested matter can be killed, and the growth of microorganisms that are attached and proliferating in the intestines can be suppressed, thereby suppressing the occurrence of digestive diseases. By including the organic acid, the digestion efficiency of proteins, etc. in the feed can be increased, and the growth of intestinal bacteria can be suppressed, thereby suppressing the occurrence of unpleasant odors in the livestock farm.

The above iron sulfate hydrate reacts with sulfur compounds and nitrogen compounds, which are the causes of odor in manure or sludge, to form ammonium salts or iron sulfide, which can play a role in removing the foul odor of hydrogen sulfide or ammonia. The manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate can continuously maintain solubility and stability without adding a separate dispersion stabilizer, and can increase the weight gain rate of livestock as a feed additive and increase the digestibility of feed. The iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate can be used to prevent diseases or reduce mortality in livestock.

The above sodium selenite is an additive that replaces selenium, and can relieve reproductive disorders and productivity decline in livestock, and suppress muscle and cardiovascular diseases. The above sodium selenite can remove active oxygen and have an antioxidant effect. The above sodium selenite has the effect of increasing the digestion efficiency of livestock when added in small amounts as a feed additive.

The step of mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction to prepare a feed additive composition is to ensure that the mineral components of the feed additive composition maintain constant solubility and uniformity regardless of pH or temperature conditions.

By mixing the first mixture, the second mixture, and the third mixture, a stronger bond than the general ionic bond of an inorganic compound is formed through a chelate bond, and a highly stable chelate substance that does not ionize or decompose until utilized in cells in a living body can be produced. Therefore, when a feed additive forming such a chelate bond is added to a feed and manufactured, there is an advantage in that it can complement problems such as conventional inorganic metal substances being ionized in organs in a living body and exhibiting toxicity, having low bioavailability, or unabsorbed substances being discharged as they are and polluting the environment. In addition, the cholesterol content of raised livestock can be lowered, and disease prevention will be possible due to improved immune function.

In the step of obtaining the first mixture, the weight ratio of the first culture medium and at least one vitamin selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E may be 1:0.1 to 0.2. If the vitamin is below the above numerical range, the immune function enhancement effect and antioxidant effect are reduced, and if it exceeds the above numerical range, the price of the feed additive increases, making it uncompetitive as a feed additive.

In the step of obtaining the second mixture, the weight ratio of the second culture medium and the sweetener may be 1:0.05 to 0.1. If the sweetener is below the above numerical range, the moisturizing function and preservative function may be weakened, and if it exceeds the above numerical range, side effects such as increased blood sugar and diarrhea may occur due to excessive sugar intake.

In the step of obtaining the third mixture, the weight ratio of the third culture solution, minerals, organic acids, iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate, and at least one hydrate selected from the group consisting of selenium and sodium selenite may be 1:0.01 to 0.1:0.1 to 0.2:0.1 to 0.3:0.01 to 0.05. When the mineral is below the numerical range, acid-alkalinity cannot be controlled and hormonal balance may not be maintained, and when it exceeds the numerical range, the bioavailability of livestock may be reduced. When the organic acid is below the numerical range, the pH in the feed and digestive organs may become too low, and when it exceeds the numerical range, the growth of microorganisms attached and proliferating in the intestines cannot be suppressed, so the occurrence of digestive diseases cannot be suppressed. If the above-mentioned hydrate is below the above-mentioned numerical range, the solubility and stability of the feed additive cannot be continuously maintained, and if the above-mentioned numerical range is exceeded, the digestibility of the feed can be inhibited. If the above-mentioned sodium selenite is below the above-mentioned numerical range, the reproductive disorders and decreased productivity of livestock cannot be resolved, and muscle and cardiovascular diseases cannot be suppressed, and if the above-mentioned numerical range is exceeded, it can accumulate in the body of livestock, cause hormonal disruption, and act as toxicity.

In the step of mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction to prepare a feed additive composition, the weight ratio of the first mixture, the second mixture, and the third mixture may be 1:1 to 5:1 to 5. If the second mixture is less than the above numerical range, the feed digestion efficiency may decrease and the moisturizing and preservative effects may decrease due to a lack of protein-decomposing enzymes, and if it exceeds the above numerical range, side effects such as increased blood sugar and diarrhea may occur. If the third mixture is less than the above numerical range, it becomes difficult to suppress harmful substances from penetrating into the intestines, making it impossible to suppress the occurrence of digestive diseases, and if it exceeds the above numerical range, it may inhibit the digestion efficiency of feed, cause hormonal disruption, and act as toxicity.

The above feed additive composition may be in liquid form.

The weight parts of the components at each step above are as follows.

In the step of obtaining the first mixture, the vitamin may be included in an amount of 0.02 to 0.2 parts by weight based on the weight part of the first culture solution, at least one selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E. Preferably, the vitamin may be included in an amount of 0.1 to 0.2 parts by weight. When the content of the vitamin is less than the numerical range, the immune function enhancement effect and the antioxidant effect are reduced, and when it exceeds the numerical range, the price of the feed additive increases, making it uncompetitive as a feed additive.

In the step of obtaining the second mixture, the sweetener may be included in an amount of 0.02 to 0.2 parts by weight based on the weight of the second culture solution. Preferably, the sweetener may be included in an amount of 0.05 to 0.1 parts by weight based on the weight of the second culture solution. If the content of the sweetener is less than the above numerical range, the moisturizing function and preservative function may be weakened, and if it exceeds the above numerical range, side effects such as increased blood sugar and diarrhea may occur due to excessive sugar intake.

In the step of obtaining the third mixture, the mineral may be included in an amount of 0.01 to 0.1 part by weight, the organic acid may be included in an amount of 0.1 to 0.2 part by weight, at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate may be included in an amount of 0.1 to 0.3 part by weight, and the sodium selenite may be included in an amount of 0.01 to 0.05 part by weight, based on the weight part of the third culture medium. When the content of the mineral is less than the numerical range, acid-alkalinity may not be controlled and hormonal balance may not be maintained, and when it exceeds the numerical range, the bioavailability of livestock may be reduced. When the content of the organic acid is less than the numerical range, the pH of the feed and digestive organs may become too low, and when it exceeds the numerical range, the growth of microorganisms attached and proliferating in the intestines may not be suppressed, so the occurrence of digestive diseases cannot be suppressed. If the content of the above-mentioned hydrate is below the above-mentioned numerical range, the solubility and stability of the feed additive cannot be continuously maintained, and if it exceeds the above-mentioned numerical range, the digestibility of the feed can be inhibited. If the content of the above-mentioned sodium selenite is below the above-mentioned numerical range, the reproductive disorders and decreased productivity of livestock cannot be resolved, and muscle and cardiovascular diseases cannot be suppressed, and if it exceeds the above-mentioned numerical range, it can accumulate in the body of livestock, cause hormonal disruption, and act as toxicity.

In the step of mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction to prepare a feed additive composition, the second mixture may be included in an amount of 1 to 2 parts by weight and the third mixture may be included in an amount of 1 to 2 parts by weight based on the weight part of the first mixture. If the content of the second mixture is less than the numerical range, feed digestion efficiency may decrease and moisturizing and preservative effects may decrease due to a lack of protein-decomposing enzymes, and if it exceeds the numerical range, side effects such as increased blood sugar and diarrhea may occur. If the content of the third mixture is less than the numerical range, it becomes difficult to suppress harmful substances from penetrating into the intestines, making it impossible to suppress the occurrence of digestive diseases, and if it exceeds the numerical range, it may inhibit feed digestion efficiency, cause hormonal disruption, and act as toxicity.

According to another embodiment of the present invention, a feed additive manufactured by any one of the above manufacturing methods is provided.

Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to explain the present invention more specifically, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention.

Example

The Streptomyces sp. microbial culture was centrifuged to separate only the liquid culture, thereby obtaining 100 g of the first culture (S1).

The Bacillus subtilis sp. microbial culture was centrifuged to separate only the liquid culture, thereby obtaining 100 g of the second culture (S2).

The Lactobacillus plantarum sp. microbial culture was centrifuged to separate only the liquid culture, thereby obtaining 100 g of the third culture (S3).

A first mixture was obtained by mixing 4 g of vitamin A, 4 g of vitamin B, 4 g of vitamin C, 4 g of vitamin D, and 4 g of vitamin E into the first culture medium at 35°C for 30 hours (S4).

A second mixture was obtained by mixing 7 g of sweetener into the second culture medium at 35°C for 30 hours (S5).

Into the third culture medium, 2 g of minerals, 15 g of organic acid were mixed, and 9 g of iron sulfate hydrate, 10 g of manganese sulfate hydrate, 2 g of copper sulfate hydrate, 1 g of cobalt sulfate hydrate, and 3 g of sodium selenite were mixed at 35°C for 30 hours to obtain a third mixture (S6).

100 g of the first mixture, 100 g of the second mixture, and 100 g of the third mixture were mixed and chelated at 50° C. for 90 minutes to prepare a feed additive composition (S7).

Experimental example

In order to determine the improved digestibility and absorption rate exhibited by the feed additive according to an embodiment of the present invention, the feed additive of the present invention was fed to 21-year-old weaned piglets and the weight gain was investigated (see Table 1).

A total of 50 21-instar weaned piglets were used, of which 25 (13 females and 12 males) were fed the feed additive manufactured in the above example, and the remaining 25 (13 females and 12 males) were fed the feed (comparative example) provided by the National Livestock Cooperatives Federation generally during breeding.

Average starting weight (kg) Average Ending Weight (kg) Weight gain per head per day (g) Example 5.12 18.70 450.2 Comparative example 5.10 17.52 412.3

Claims (5)

A step of obtaining a first culture solution by centrifuging a Streptomyces sp. microorganism culture solution and separating only the liquid culture solution;
A step of centrifuging a Bacillus subtilis sp. microbial culture to separate only the liquid culture solution and obtain a second culture solution;
A step of centrifuging a Lactobacillus plantarum sp. microbial culture solution and separating only the liquid culture solution to obtain a third culture solution;
A step of obtaining a first mixture by mixing at least one vitamin selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E into the first culture solution;
A step of mixing a sweetener into the second culture medium to obtain a second mixture;
A step of mixing minerals and organic acids in the third culture solution and mixing sodium selenite with at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate to obtain a third mixture; and
A method for producing a feed additive, comprising the step of mixing the first mixture, the second mixture, and the third mixture and performing a chelating reaction to produce a feed additive composition.
In claim 1,
A method for manufacturing a feed additive, wherein, in the step of obtaining the first mixture, the weight ratio of the first culture solution and at least one vitamin selected from the group consisting of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E is 1:0.1 to 0.2.
In claim 1,
A method for manufacturing a feed additive, wherein in the step of obtaining the second mixture, the weight ratio of the second culture solution and the sweetener is 1:0.05 to 0.1.
In claim 1,
A method for manufacturing a feed additive, wherein in the step of obtaining the third mixture, the weight ratio of the third culture solution: mineral: organic acid: at least one hydrate selected from the group consisting of iron sulfate hydrate, manganese sulfate hydrate, copper sulfate hydrate, and cobalt sulfate hydrate: sodium selenite is 1:0.01 to 0.1:0.1 to 0.2:0.1 to 0.3:0.01 to 0.05.
A feed additive manufactured by any one of the manufacturing methods of claims 1 to 4.