KR100840145B1 - Fermented feed for animal husbandry using lactic acid bacterium and yeast and method for producing the same - Google Patents

Abstract

The present invention relates to a fermented feed for livestock production using lactic acid bacteria and yeast, and more particularly, to a fermented feed for livestock using lactic acid bacteria and yeast capable of surviving at high temperature and low pH, and a method for producing the fermented feed.

When the fermented feed according to the present invention is fed to livestock such as cattle, dairy cows, pigs, poultry, etc., it is possible to improve the quality of the poultry farming, Can contribute greatly to improvement, and more importantly, enable environmentally friendly biomass production without the use of antibiotics or growth promoting agents in livestock.

Description

[0001] The present invention relates to a fermented feed for livestock production using lactic acid bacteria and yeast, and a method for producing the fermented feed. [0002] Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method [

The present invention relates to a fermented feed for livestock production using lactic acid bacteria and yeast, and more particularly, to a fermented feed for livestock using lactic acid bacteria and yeast capable of surviving at high temperature and low pH, and a method for producing the fermented feed.

Over the past several decades, our livestock industry has been using antibiotics to promote livestock prophylaxis, treatment, and growth of livestock, improve feed efficiency, and improve livestock productivity. However, the use of antibiotics has been gradually regulated for the purpose of promoting growth, since the microorganisms resistant to the antibiotics are increased and the effectiveness of the antibiotics is deteriorated or remains in the livestock products and adversely affects the human body have. In reality, the problem of remaining foreign matter in livestock products is often causing water pollution, and it is a direct obstacle to the export of pork meat to Japan. Therefore, an alternative to antibiotics is urgently needed.

As an alternative to solve this problem, fermented feedstuffs using microorganisms or mostly single fungi mainly based on fungi (yeast, mold) are suggested. However, considering the economic benefits of livestock farming, the nutritional control of the growth of livestock, the prevention of diseases and the reduction of stress, by reducing the feed efficiency by reducing the amount of feed, reducing the mortality rate, Complex microbial combinations composed of beneficial functional microorganisms are more efficient.

Polymeric materials produced during soybean fermentation can be roughly divided into γ-polyglutamate (γ-polyglutamate) and polyfructan (polyfructan). Since γ-polyglutamate is known to have a moisturizing, anti-inflammatory and cell activating function, it can be used as a raw material for cosmetics. In the pharmaceutical industry, when it is used together with taxol and other cancer drugs, . Polyfructans are present with γ-polyglutamate and act to amplify functions such as moisturizing and anti-inflammation. Levan, a microbial polyfructan, is a diet fiber for diet that can suppress the appetite by giving fullness to the human body. It is reported to inhibit the proliferation of harmful bacteria in the intestines and to help the growth of lactic acid bacteria have.

An example of a fermented soybean curd so far used in the industry is Korean Patent No. 0003751 (a method for producing fermented feeds using agricultural wastes).

It is an object of the present invention to provide a fermented feed for livestock that can be used for eco-friendly biomass using lactic acid bacteria and yeast, and a method for producing the fermented feed.

In order to accomplish the above object, the present invention provides a method for producing Lactobacillus fermentum JS (KCCM 10499), Pichia kluyveri , Saccharomyces cerevisiae cereviseae ). The present invention also provides a fermented feed composition for animal husbandry.

The present invention relates to the use of Lactobacillus < RTI ID = 0.0 > fermentum JS (KCCM 10499) and Pichia kluyveri ), Saccharomyces cerevisiae cereviseae ) is inoculated into a medium containing spent substrate from mushroom cultivation, rice bran, large scalp, and silkworm to ferment the fermented feed composition.

The present invention can provide a fermented feed for animal husbandry and a method for producing the fermented feed using lactic acid bacteria and yeast. When the fermented feed according to the present invention is fed into cattle feed such as bovine feed, pig or poultry feed, effects such as growth, feed reduction and prevention of diarrhea can be effected, as well as high quality meat production and improvement of housing environment. Can greatly contribute.

The present invention will be described in detail as follows.

The present invention has good effects on swine and poultry by using Lactobacillus fermentum JS (KCCM 10499), Pichia kluyveri , Saccharomyces cereviseae strain, A fermented feed composition for environmentally-friendly biotechnology can be prepared.

The fermented feed composition of the present invention may further comprise at least one selected from the group consisting of Lactobacillus fermentum JS (KCCM 10499), Pichia kluyveri , Saccharomyces cereviseae , It is preferable to inoculate the fermented product in a medium containing rice bran, large scalp and calamus.

Preferably, the fermentation is performed at 35-50 ° C for 24-72 hours.

Preferably, the medium comprises 60-80% of mushroom waste medium, 5-20% of rice bran, 3-10% of scalp, 2-10% of rice husk, based on the total weight.

In addition, the culture medium may further contain 5-35% molasses or 3-12% glucose in relation to the total weight.

In the present invention, the term "mushroom waste medium" refers to a mushroom medium which is discarded after mushroom cultivation and after the mushroom has been cultivated. Usually mushroom culture uses oak or pine sawdust as mushroom culture medium, After the mushroom has been cultivated for a certain period of time, the mushroom is collected. Then, the mushroom medium contained in the plastic bottle is purged from the outside by air It is called a mushroom pulp and is a commonly used term in the field of mushroom cultivation.

Examples of the mushroom waste medium include waste media such as mushroom mushroom, shiitake mushroom, oyster mushroom, mushroom mushroom, mushroom mushroom, and sea mushroom mushroom, but the mushroom culture medium can be used without limitation.

For the present invention, strains of Lactobacillus fermentum JS, Pichia culliberi, and Saccharomyces cerevisiae are cultured in respective media, and then the cells are separated using a centrifuge. It is preferable to mix the separated cells with dextrin or skim milk powder, freeze at -80 ° C, and dry the powder in a freeze-dryer to prepare a powder.

It is preferable that the fermented feed composition is prepared by mixing Lactobacillus perfumant JS lactic acid bacteria with Pichia culberryberry or Saccharomyces cerevisiae yeast.

The fermented feed composition preferably contains 1 × 10 ⁵ CFU / g to 1 × 0 ¹¹ CFU / g of each strain.

The present invention can be suitably mixed with the fermented feed composition to produce a feed composition for livestock.

5 to 30% of wheat, 5 to 30% of protein, 4 to 15% of molasses, 5 to 10% of glucose, 0.2 to 1.5% of phosphoric acid, calcium of calcium 0.3-0.5% of the compound, 0.1-0.5% of the complex vitamin, 0.5-3% of the limestone, 0.1-1% of the salt, and fermented at 35-50 ° C for about 30 minutes to 10 hours with the fermenter to obtain the feed composition (1) , And the feed composition (1) is preferably used as a breeding feed.

40-70% of the feed composition (1) obtained above, 10-35% of compressed corn, 8-20% of lupine beans, 2-6% of protein, 1.5-5% of wheat bran, 3-12% of molasses, , Fermented at 35-50 ° C for about 30 minutes to 10 hours with a fermenter mixed with 0.1% -8%, 0.1-0.5% mixed vitamins, 0.2-1.5% phosphoric acid, 0.3-2.5% calcium and 0.1-0.5% 2), and the feed composition (2) is preferably used as a poultry feed.

15-40% of the feed composition (1), 25-50% of pressed corn, 20-40% of lupine beans, 1-5% of protein, 1-5% of wheat bran, 4-13% of molasses, 5-9 %, And fermented with a fermenter at 35-50 ° C for about 30 minutes to 10 hours to obtain a feed composition (3), and the feed composition (3) is preferably used as a late meal for finishing.

The limestone used in the present invention is a feed limestone used for calcium supplementation of the compounded feed, and it is preferable that the content of CaCO ₃ is higher and it has a particle size of 100-200 mesh.

According to the present invention, the fermented feed composition can be fed to livestock to produce a good quality meat meal.

In the above, the livestock may be selected from pigs, cows, chickens, and ducks.

According to the embodiment of the present invention, in the case of the beef cattle, when the fermented feed is fed and raised, it is possible to produce the ginseng effect and the high quality meat, and the feed cost can be reduced by 30% or more. In addition, the incidence of disease is 0% by feeding fermented feed, and the odor of the house is remarkably reduced by decomposing ammonia, which is one of the characteristics of lactic acid bacteria, which is the root cause of odor, and eco-friendly biomass It is possible.

Lactobacillus fermentum JS used as a fermentation strain in the present invention is characterized by strong resistance to heat and acid, unlike the weak characteristic of heat and acid possessed by conventional lactic acid bacteria, and has an effect of decomposing ammonia (NH ₃ ). In the case of a livestock breeding, the internal temperature (product temperature) at the time of fermentation rises to about 85 ° C, and the usual lactobacillus is killed, but the lactobacillus fermentum JS strain grows rather that the population is increased It secretes various functional substances from the raw material of the medium, and at the same time, decomposes the fibrous material into a digestible state and helps digestion of livestock.

In addition, the yeast Pichia culberryberry is a naturally occurring strain when fermenting wine, and is known to be mainly responsible for wine fermentation. Saccharomyces cerevisiae is mainly used for fermenting bread, Can contain nutrients rich in proteins, vitamins, and minerals in the cell, making it a nutrient source for livestock. These yeasts can be co-cultivated with Lactobacillus fermentum JS strain and can be cultivated simultaneously in the same medium, thereby giving various advantages of the fermented feed. In other words, it is expected to provide various nutritional substances, digestion and absorption as a probiotic agent, activation of intestinal beneficial microflora, and immunity enhancement through the production of functional substances.

Hereinafter, the present invention will be described more specifically with reference to the following examples. However, these embodiments have been proposed to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Fermented feed, breeding period feed, and non-breeding late feed production process

Fermented feed, breeder feed, and post - breeder feed were prepared. The respective production processes are shown in FIG. 1 briefly.

Lactobacillus fermentum JS and Saccharomyces cereviseae strains were used for the preparation of the fermented feed.

Lactobacillus fermentum JS was prepared by inoculating Lactobacillus fermentum JS strain to MRS medium and culturing at 40 ° C for 1 to 7 days. Add 50 liters of water to a 50 liter culture tank, sterilize at 121 ° C for 10-15 minutes, and cool the water to 30-45 ° C. Then, as the medium composition, 5-15 g of protease peptone No. 3, 3-10 g of beef extract, 10-30 g of glucose, 3-15 g of sodium acetate, , And 1-8 g of ammonium citrate are put in a culture tank and diluted. 40 ml of sterilized water was aseptically put into a 50 ml flask, and the lactobacillus fermentum JS strain cultured in the MRS medium was inoculated into the flask, diluted, and then placed in a culture tank and cultured at 30-45 ° C for 1-3 days. When the number of lactic acid bacteria in the culture solution reaches 10 ⁹ CFU / g or more, the cells are separated using a centrifuge and mixed with dextrin or skimmed milk so that the volume becomes 3 ml per 1 liter of the stock solution. After freezing at -80 ° C., Powder form.

Cultivation of yeast Saccharomyces cerevisiae is carried out by adding 2-5 g of yeast extract, 2-5 g of malt extract, 3-7 g of peptone, 5 g of glucose 5- And sterilized. Yeast cells previously cultured in a YM liquid medium were inoculated at a concentration of about 1-3% and cultured at 25 DEG C for 5 days. The cultured yeast cells were separated from the cells using a centrifugal separator, mixed with dextrin or skimmed milk to make 3 ml per 1 liter of the stock solution, and then frozen at -80 ° C and dried in a freeze dryer to prepare a powder.

Mixed Lactobacillus fermentum JS and yeast Saccharomyces cerevisiae were mixed to adjust the number of bacteria to be 10 ⁵ CFU / g or more, and mixed with glucose, rice bran, lactose, and skim milk to make a fermented feed .

The mixing ratios of fermented feed, breeding period feed, and latefeeding feed are shown in Tables 1 to 3.

<Table 1> Fermented feed composition (%)

division Raw material name Formulation ratio (%) Fermented feed Oyster mushroom waste paper 70 Rice bran 10 Scalp 10 깻 10 system 100

<Table 2> Feed composition feed composition (%)

division Raw material name Formulation ratio (%) Feeding period (4-13 months) Fermented feed 65 Wavefront 10.5 Wheat flour 10.0 Protein blood 10.0 Phosphorus potassium 1.0 vitamin 0.5 Limestone 2.0 Salt 1.0 system 100

<Table 3> Feeding composition of late fattening and finishing (%)

Raw material name Feeding fodder (14-22 months) Fertilizer feedstuffs (23-28 months) Feeding period feed 59 35 Flake 20 30 Lupine 10.0 30 Protein blood 5.0 2.5 Wheat flour 5.0 2.5 vitamin 0.5 0 Phosphorus potassium 0.3 0 Limestone 0.2 0 system 100 100

<Example 2> Analysis of components of fermented feed and breeding period feed

The components of the fermented feed prepared in Example 1 and the fermented feed in the breeding period were analyzed. As a result, as shown in Table 4 and Table 5, the carbohydrate content of the fermented feed in the breeding period was low, while the content of crude protein and crude fat was higher than that of the fermented feed.

<Table 4> Analysis of fermented feed

Analysis item Analysis moisture 54.29% Crude protein 3.31% Crude fat 2.07% Crude fiber 19.06% Views min 3.46% Soluble nitrogen-free water 17.81% carbohydrate 36.87% Calorie 2,259㎉ / kg Number of lactic acid bacteria 3.2 × 0 ⁷ cfu / g Yeast count 6.4 x 10 &lt; ⁸ &gt; cfu / g

<Table 5> Analysis of fermented feed in breeding period

Fermented feedstuffs of lactic acid bacteria used as auxiliary feed

Analysis item Analysis moisture 56.11% Crude protein 6.59% Crude fat 3.55% Crude fiber 11.28% Views min 3.04% Soluble nitrogen-free water 19.43% carbohydrate 30.71% Calorie 2,300 ㎉ / ㎏ Number of lactic acid bacteria 3.2 x 10 &lt; ⁷ &gt; cfu / g Yeast count 6.4 x 10 &lt; ⁸ &gt; cfu / g

&Lt; Example 3 > Experimental results of pigs fed with supplementary feeds

The control diet and the fermented feed were supplemented with 10% of the supplementary feed, and were fed with 40 piglets for 17 weeks. 120 piglets were fed for 17 weeks, and weight gain and feeding rate of pigs were measured. As a result, the average daily gain of the control was 564.6g, while that of the supplementary feed was 611.9g, which showed a difference of 47.3g. As for the feed conversion ratio, the feedstock was 1.65kg per day, while the supplementary feedstock was 1.61kg, which was 40g lower, indicating that the feed efficiency was higher.

Lactic acid bacteria and yeast were used to prepare fermented diets, and the diets were added to the diets. As a result, the diets were able to be shipped at a standard weight of about 100-110 kg at 5 months of age.

&Lt; Example 4 >

120 piglets were observed during the 17 weeks of feeding. The diarrhea index of pigs showed that diarrhea of 15 diarrhea was significantly higher than that of the control group. In addition, two pigs out of 40 pigs died due to diarrhea and other diseases as a control group, but there were no mortalities in the experimental group fed with supplementary feed containing lactic acid bacteria yeast.

&Lt; Example 5 > Poultry: 420 counts of broiler, 2 times of 32 days

420 poultry were raised for 32 days, and the weight gain and feeding rate were measured. For the control, general commercial diets were used, and supplemented diets were supplemented with 10% of fermentation diets for general diets. The average feed weight of the broiler chicks fed the fermented diets was 90g higher than that of the control diet, which was 1.89kg for the control diet and 1.77kg for the supplementary diet.

&Lt; Example 6 >

The Lactobacillus fermentum JS and Saccharomyces cereviseae of the present invention can be produced by a method comprising the steps of: The fermented diets and common diets were used to breed 4 - month old cattle for 28 months. In case of breeding with fermented feed, the feed prepared in Example 1 was supplied. Feeds were fed for up to 4-13 months, feeder for 14-22 months, and feeder for 23-28 months. As a result, the fermented feedlot received 91.4% of the grade 1 or higher in the grade of Hanwoo carcass (Table 6).

<Table 6> Comparative analysis of fermented feedstuffs and cattle fed cattle feed

Item 46 wells fed with fermented feed Forty-two cattle raised with compound feed Comparison value Weight B grade or higher: 80.5% C grade: 19.5% B grade or higher: 78% C grade: 22% Class B or higher: 2.5% Class C: 2.5% Meat Class 1 or higher: 91.4% Class 2 or lower: 8.6% Class 1 or higher: 73% Class 2 or lower: 27% Class 1 or higher: 18.4% Class 2 or lower: 18.4% Body weight (kg) 683 685 Combined feed Hanwoo 2kg Weight (kg) 410 398 Fermented feed Hanwoo 12kg Conductivity (%) 60.1 58.1 Fermented feed Hanwoo 2% Feed Cost Saving Rate (%) 30 or more 0 Based on compound feed Advantages 1. Pleasant environment 2. No disease (reduction of treatment cost) 3. Growth is good 4. Almost no odor 5. Pest less

<Example 7> Reduction of feed cost of fermented feed

In order to investigate the effect of reducing the feed cost of fermented feed, The feeding cost savings per cattle during 23 months of cattle feeding were calculated. As a result, as shown in Tables 7 to 9, the fermented feed diets were reduced by 31.7% and 23.8%, respectively, compared to the general formula feeds of Company P and Company C, respectively.

<Table 7> Fermented mixed feed

Step-by-step feed Price per kg (won) Price per bag (KRW / 25kg) Requirement (kg) (turret) Required amount (won) Breeding machine 160 4,000 1,375 (55) 220,000 Fattening electricity 198 4,950 2,250 (90) 445,500 The latter 216 5,400 2,100 (84) 453,600 Sum 5,725 (229) 1,191,100

<Table 8> P Company General compound feed

Step-by-step feed Price per kg (won) Price per bag (KRW / 25kg) Requirement (kg) (turret) Required amount (won) Breeding machine 317 8,420 1,080 (43) 342,360 Fattening electricity 340 9,000 1,875 (75) 637,500 The latter 352 9,290 2,100 (84) 739,200 Sum 5,055 (202) 1,719,060

<Table 9> C company general compound feed

Step-by-step feed Price per kg (won) Price per bag (KRW / 25kg) Requirement (kg) (turret) Required amount (won) Breeding machine 290.8 7,270 1,065 (43) 309,702 Fattening electricity 293.6 7,340 2,025 (81) 594,540 The latter 314 7,850 2,100 (84) 659,400 Sum 5,190 (208) 1,563,642

1 shows a fermented feed, a breeding period feed, a poultry and a late feed production process.

Claims (8)

delete Lactobacillus fermentum JS (KCCM 10499), Pichia culberi or Lactobacillus fermentum JS (KCCM 10499) and Saccharomyces cerevisiae were added to the medium in an amount of 60 to 80% of mushroom waste medium, %, A scalp of 3 to 10% and a spoon of 2 to 10%, and fermenting at 35 to 50 DEG C for 24 to 72 hours. The fermented feed composition of claim 2, wherein the medium further comprises 5 to 35% molasses or 3 to 12% glucose. The feed composition according to claim 2, which comprises 35-65%, crushed corn 5-25%, wheat 10-30%, protein 5-30%, molasses 4-15%, glucose 5-10%, phosphoric acid 0.2 Wherein the composition is fermented by mixing 1 to 1.5% of calcium, 0.3 to 2.5% of calcium, 0.1 to 0.5% of a complex vitamin, 0.5 to 3% of limestone, and 0.1 to 1% of salt. 40-70% of the feed composition of claim 4, 10-35% of corn crushed, 8-20% of lupine beans, 2-6% of protein, 1.5-5% of wheat bran, 3-12% of molasses, 3-8% , 0.1-0.5% of a complex vitamin, 0.2-1.5% of phosphoric acid, 0.3-2.5% of calcium, and 0.1-0.5% of limestone are mixed and fermented. 15-40% of the feed composition of claim 4, 25-50% of pressed corn, 20-40% of lupine beans, 1-5% of protein, 1-5% of wheat bran, 4-13% of molasses, 5-9% Are mixed and fermented. A method of producing meat meal, characterized in that the feed composition of any one of claims 1 and 4 to 6 is fed to livestock. 8. The method according to claim 7, wherein the livestock is at least one selected from pigs, cows, chickens and ducks.

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