CN111616259A - Production method of fermented dry feed capable of fully playing material adsorption role - Google Patents
Production method of fermented dry feed capable of fully playing material adsorption role Download PDFInfo
- Publication number
- CN111616259A CN111616259A CN202010491248.8A CN202010491248A CN111616259A CN 111616259 A CN111616259 A CN 111616259A CN 202010491248 A CN202010491248 A CN 202010491248A CN 111616259 A CN111616259 A CN 111616259A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- culture
- temperature
- dry feed
- peptone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000463 material Substances 0.000 title claims abstract description 63
- 238000001179 sorption measurement Methods 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 134
- 230000004151 fermentation Effects 0.000 claims abstract description 134
- 241000894006 Bacteria Species 0.000 claims abstract description 50
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 48
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims abstract description 44
- 238000001035 drying Methods 0.000 claims abstract description 44
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 41
- 239000008103 glucose Substances 0.000 claims abstract description 41
- 229910052901 montmorillonite Inorganic materials 0.000 claims abstract description 41
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 31
- 240000008042 Zea mays Species 0.000 claims abstract description 30
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 30
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 30
- 235000005822 corn Nutrition 0.000 claims abstract description 30
- 239000004310 lactic acid Substances 0.000 claims abstract description 24
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 6
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 54
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 48
- 239000001888 Peptone Substances 0.000 claims description 47
- 108010080698 Peptones Proteins 0.000 claims description 47
- 235000019319 peptone Nutrition 0.000 claims description 47
- 230000001954 sterilising effect Effects 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 39
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 36
- 239000000758 substrate Substances 0.000 claims description 32
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 31
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 31
- 229940041514 candida albicans extract Drugs 0.000 claims description 30
- 239000012138 yeast extract Substances 0.000 claims description 30
- 235000019764 Soybean Meal Nutrition 0.000 claims description 26
- 239000004455 soybean meal Substances 0.000 claims description 26
- 238000012258 culturing Methods 0.000 claims description 25
- 239000011780 sodium chloride Substances 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 21
- 229920001817 Agar Polymers 0.000 claims description 18
- 239000008272 agar Substances 0.000 claims description 18
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 18
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 18
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 18
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 18
- 238000011081 inoculation Methods 0.000 claims description 18
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 18
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 18
- 241000186660 Lactobacillus Species 0.000 claims description 17
- 229940039696 lactobacillus Drugs 0.000 claims description 17
- 239000008213 purified water Substances 0.000 claims description 17
- 238000012136 culture method Methods 0.000 claims description 15
- 235000013312 flour Nutrition 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- 238000011218 seed culture Methods 0.000 claims description 13
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 235000015278 beef Nutrition 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 229960004793 sucrose Drugs 0.000 claims description 12
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 239000002518 antifoaming agent Substances 0.000 claims description 6
- 238000009630 liquid culture Methods 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000005995 Aluminium silicate Substances 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 4
- 239000004113 Sepiolite Substances 0.000 claims description 4
- 229910021536 Zeolite Inorganic materials 0.000 claims description 4
- 235000012211 aluminium silicate Nutrition 0.000 claims description 4
- 229960000892 attapulgite Drugs 0.000 claims description 4
- 239000000440 bentonite Substances 0.000 claims description 4
- 229910000278 bentonite Inorganic materials 0.000 claims description 4
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 4
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 4
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 4
- 229910052625 palygorskite Inorganic materials 0.000 claims description 4
- 229910052624 sepiolite Inorganic materials 0.000 claims description 4
- 235000019355 sepiolite Nutrition 0.000 claims description 4
- 239000011257 shell material Substances 0.000 claims description 4
- 239000010457 zeolite Substances 0.000 claims description 4
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 3
- 241000194032 Enterococcus faecalis Species 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 2
- 241000194031 Enterococcus faecium Species 0.000 claims description 2
- 241001106597 Enterococcus lactis Species 0.000 claims description 2
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 2
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 2
- 244000199866 Lactobacillus casei Species 0.000 claims description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 2
- 241000186673 Lactobacillus delbrueckii Species 0.000 claims description 2
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 2
- -1 Maifanitum Chemical compound 0.000 claims description 2
- 241000191998 Pediococcus acidilactici Species 0.000 claims description 2
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 2
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims description 2
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 2
- 229940017800 lactobacillus casei Drugs 0.000 claims description 2
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 239000002207 metabolite Substances 0.000 abstract description 10
- 241001465754 Metazoa Species 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 5
- 206010012735 Diarrhoea Diseases 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 244000144972 livestock Species 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 244000144977 poultry Species 0.000 abstract description 2
- 239000006041 probiotic Substances 0.000 abstract description 2
- 235000018291 probiotics Nutrition 0.000 abstract description 2
- 238000010563 solid-state fermentation Methods 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 239000007787 solid Substances 0.000 description 13
- 239000002253 acid Substances 0.000 description 11
- 210000001035 gastrointestinal tract Anatomy 0.000 description 7
- 238000010899 nucleation Methods 0.000 description 7
- 229910021647 smectite Inorganic materials 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
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- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
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- 230000002062 proliferating effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
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- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A23V2400/113—Acidophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2400/137—Delbrueckii
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23V2400/413—Acidilactici
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2400/427—Pentosaceus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2400/00—Lactic or propionic acid bacteria
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- A23V2400/517—Bifidum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention relates to a production method of a fermented dry feed, in particular to a production method of a fermented dry feed which can fully play a role of adsorbing materials. The method comprises the steps of taking probiotics such as saccharomycetes, bacillus and lactic acid bacteria as strains, taking glucose, bean pulp, corn, montmorillonite and the like as main raw materials, carrying out solid state fermentation culture, drying the fermented wet feed at low temperature, keeping metabolites of the fermented feed to the maximum extent in the fermentation and drying processes through the adsorption effect of the montmorillonite, and crushing to produce a fermented dry feed product with the pH value of 5.6-6.6, the water content of less than or equal to 10%, the ash content of less than or equal to 50% of 5%, the granularity of more than or equal to 20 meshes, the special light gray or gray black color and the quality guarantee period of 6 months. The product of the invention is applied to livestock and poultry and aquaculture, can obviously reduce the occurrence of animal diarrhea, improves the production performance of animals, and can improve the environment. Meanwhile, the product has the advantages of convenient use, easy storage, long product quality guarantee period and the like.
Description
Technical Field
The invention relates to a fermented dry feed product, in particular to a production method of fermented dry feed which fully exerts the montmorillonite function.
Background
Through the efforts of vast microbial workers in the last ten years, the fermented feed becomes an industry with huge vitality in the feed industry, plays an increasingly important role in the field of livestock breeding, and is paid more and more attention by people particularly since the resistance of the feed industry is forbidden, so that the development of the fermented feed industry is further promoted. Fermented feed is a process in which a substrate is degraded while it is proliferating and a metabolite is produced by the action of microorganisms. As a considerable part of the microbial fermentation metabolites are volatile substances, the volatile metabolites can be greatly volatilized in the processes of fermentation and subsequent fermented feed drying, so that the loss of effective microbial fermentation products is caused, and the effective performance of the fermented feed is reduced. According to the invention, montmorillonite is adopted to participate in fermentation, and the montmorillonite is combined with a microbial fermentation metabolite by virtue of the strong adsorption performance of the montmorillonite, so that the volatilization loss of the volatile metabolite of the fermented feed in the fermentation and drying processes is effectively reduced, and the performance of the fermented feed is maintained to the greatest extent. Meanwhile, the effective combination of the montmorillonite and the beneficial bacteria in the fermented feed can protect the intestinal tract more effectively. Under the large environment of resistance reduction, resistance limitation and resistance prohibition, the invention is also necessarily beneficial to sustainable and healthy development of the breeding industry in China.
Disclosure of Invention
The invention aims to provide a production method of fermented dry feed which fully plays a role of adsorbing materials.
In order to achieve the purpose, the invention adopts the technical scheme that: taking probiotics such as saccharomycetes, bacillus, lactobacillus and the like as strains, and fermenting to obtain lactobacillus seed liquid through primary solid seed culture and secondary and tertiary liquid seed fermentation culture. Glucose, bean pulp, corn, montmorillonite and the like are used as main production raw materials, anaerobic solid state fermentation culture is carried out, the fermented wet feed is dried at low temperature, metabolites of the fermented feed can be greatly reserved in the fermentation and drying processes through the adsorption effect of the montmorillonite, and the fermented dry feed product with the pH value of 5.6-6.6, the moisture content of less than or equal to 10 percent, the granularity of more than or equal to 20 meshes, the color and luster of a special light gray or gray black and the quality guarantee period of 6 months is produced through crushing.
A production method of fermented dry feed which fully plays a role of adsorbing materials is characterized by comprising the following steps:
(1) preparing a fermentation liquid strain;
(2) preparing adsorption materials, and mixing to prepare a fermentation substrate;
(3) adding the strain of the fermentation liquid prepared in the step (1) into the fermentation substrate prepared in the step (2) for inoculation and mixing (the weight ratio of the fermentation liquid strain to the sum of the fermentation substrate and the adsorption material is 1:1-4), mixing to obtain a wet material with the humidity of 30-50%, and fermenting at the temperature of 25-40 ℃ for 48-96 hours;
(4) drying the material obtained by fermentation in the step (3) containing the adsorption material at low temperature to obtain a dry finished product, wherein the moisture of the dry finished product is controlled to be 8-15%;
(5) crushing the dried finished product prepared in the step (4);
the fermentation liquid strain in the step (1) comprises saccharomycetes, bacillus and lactic acid bacteria, and the ratio of the viable count of the saccharomycetes, the bacillus and the lactic acid bacteria in the fermentation liquid strain is 1: (1-5) and (1-5);
the fermentation substrate in the step (2) comprises 1-3 parts by weight of glucose, 10-20 parts by weight of soybean meal, 10-20 parts by weight of corn flour and 10-70 parts by weight of bran; 10-70 parts of prepared adsorption material; (preferably 2 parts of glucose, 20 parts of bean pulp, 20 parts of corn flour, 40 parts of bran and 20 parts of adsorption material);
the adsorption materials are as follows: any one or more of montmorillonite, Maifanitum, zeolite, bentonite, kaolin, shell powder, sepiolite and attapulgite.
The adding time of the adsorption materials is as follows: the fermentation effect is best when the fermentation substrate and the fermentation seed liquid are mixed within 1-48 hours, the fermentation effect is next to be added within more than 48-96 hours after the fermentation is started, and the fermentation substrate and the fermentation seed liquid are mixed again within more than 96 hours after the fermentation is started-before the fermentation is finished or before the drying.
And (2) respectively and independently adding the fermentation strains in the step (1) into the fermentation substrate prepared in the step (2) for inoculation and mixing or adding the fermentation strains in the step (1) into the fermentation substrate prepared in the step (2) after mixing.
The bacillus is one or a mixture of two of bacillus subtilis and bacillus licheniformis in any proportion.
The yeast is one or a mixture of two of candida utilis and saccharomyces cerevisiae in any proportion.
Preferably, the lactobacillus is one or a mixture of lactobacillus acidophilus, lactobacillus casei, lactobacillus delbrueckii subsp lactis, lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, bifidobacterium bifidum, enterococcus faecalis, enterococcus faecium, enterococcus lactis, lactobacillus fermentum and lactobacillus bulgaricus in any proportion.
The total viable count of the zymophyte liquid prepared in the step (1) is 1.0 × 109～1.0×1010One/ml.
The fermentation strain prepared in the step (1) specifically comprises the following steps:
the fermentation liquid strain is yeast, bacillus, lactobacillus;
the culture method of the yeast comprises the following steps:
slant culture medium and culture conditions of yeast: 2.0 percent of glucose, 1.0 percent of peptone, 0.5 percent of yeast extract and 2.0 percent of agar, the pH value is 6.8, and the sterilization is carried out for 18 minutes at the temperature of 115 ℃; culturing at 30 deg.c for 24 hr;
the shake flask seed culture medium and culture conditions of the yeast are as follows: 2.0% of glucose, 1.0% of peptone and 0.5% of yeast extract; sterilizing at 115 deg.C for 18min at an initial pH of 6.8; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for expanding propagation of the microzyme seeding tank are as follows: 5.0% of culture medium soybean meal, 5% of corn, 2.0% of cane sugar, 1.0% of peptone, 0.5% of yeast extract, 0.1% of monopotassium phosphate, 0.2% of ammonium sulfate, 0.05% of sodium chloride and 0.05% of defoaming agent, sterilizing at the initial pH of 6.8 and 121 ℃ for 30 minutes, cooling to 30 ℃, inoculating the seed solution in the shake flask into a seed tank according to the proportion of 5% of the inoculum size, and culturing at the tank pressure of 0.05Mpa, the rotating speed of 50rpm and the temperature of 30 ℃ for 24 hours.
Preferably, the bacillus is cultured by the following method:
the bacillus slant culture medium and culture conditions are as follows: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, agar 2.0%, pH7.0, sterilizing at 115 deg.C for 18 min; culturing at 30 deg.C for 24 h;
the bacillus shake flask seed culture medium and culture conditions are as follows: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, pH7.0, placing in 30 deg.C constant temperature shaking table, and shake culturing at 200r/min for 24 h;
the bacillus seed tank propagation culture medium and culture conditions are as follows: 2.0 percent of soybean meal, 2.0 percent of corn, 2.0 percent of cane sugar, 0.4 percent of ammonium sulfate, 0.1 percent of ammonium chloride, 7.0 percent of PH, 30 minutes of sterilization at 121 ℃, cooling to 30 ℃, inoculating seed liquid in a shake flask into a seeding tank according to the proportion of 5 percent of inoculum size, wherein the tank pressure is 0.05Mpa, the rotating speed is 50rpm, and the culture time is 24 hours at 30 ℃.
Preferably, the culture method of the lactic acid bacteria comprises the following steps:
lactic acid bacteria slant culture medium and culture conditions: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, pH7.0 and agar 2.0%; 115 ℃ for 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The liquid culture medium and culture conditions of lactobacillus in the solanum shaped bottle are as follows: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, wherein the pH value is 7.0, the temperature is 115 ℃ and the time is 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The lactobacillus seeding tank propagation culture medium and the culture conditions are as follows: 1.0% of glucose, 0.5% of peptone, 0.5% of yeast powder, 2.0% of corn flour, 2.0% of soybean meal, 0.2kg of ammonium sulfate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.10% of dipotassium hydrogen phosphate and the balance of purified water and pH 6.8; and (3) sterilization conditions: 0.1MPa, 121 ℃ and 20 min; the fermentation culture conditions comprise a tank pressure of 0.05Mpa, a culture temperature of 33 ℃ and a time of 48 h.
Preferably, the material obtained by fermentation is dried at low temperature, and the drying treatment conditions are as follows: and drying by adopting a drying furnace, wherein the temperature in the drying furnace is 60-80 ℃, the outlet temperature of the drying furnace is 38-42 ℃, and the time is 8-10 min.
Preferably, the crushing treatment is carried out under sterile and clean conditions, and the granularity after crushing is 20-40 meshes.
The invention adds the adsorption materials of montmorillonite, medical stone, zeolite, bentonite, kaolin, shell powder, sepiolite and attapulgite, so the fermented dry feed obtained by the invention is different from the conventional feed in ash source, and the ash range is 5% to 50%.
The invention has the beneficial effects that:
by utilizing the good adsorption property of the montmorillonite, the characteristic of firm combination of the montmorillonite and the fermentation product is exerted, so that the volatilization loss of the fermentation product can be effectively reduced in the fermentation and drying processes of the fermented feed, and the fermentation product of the fermented feed is retained to the maximum extent to achieve the maximum efficiency. Meanwhile, the montmorillonite can effectively adsorb and fix toxin generated by pathogenic bacteria in the digestive tract and inhibit the pathogenic performance of the toxin, so that the digestive tract is protected from being damaged, and the montmorillonite can absorb toxic gas in the intestinal tract to effectively keep the intestinal tract healthy. The montmorillonite can better protect the intestinal tract and repair intestinal tract wounds, prevent and treat diarrhea, promote the health and the rapid growth of cultured animals and improve the culture benefit by cooperating with beneficial bacteria in the fermented feed.
And through the adsorption effect of the montmorillonite, the metabolite of the fermented feed can be retained to the maximum degree in the fermentation and drying processes, and the fermented dry feed product with the pH value of 5.6-6.6, the water content of less than or equal to 10%, the ash content of less than or equal to 50% of 5%, the granularity of more than or equal to 20 meshes, the specific light gray or gray black color and the quality guarantee period of 6 months is produced through crushing. The product of the invention is applied to livestock and poultry and aquaculture, can obviously reduce the occurrence of animal diarrhea, improves the production performance of animals, and can improve the environment. Meanwhile, the product has the advantages of convenient use, easy storage, long product quality guarantee period and the like.
Detailed Description
In order to better understand the present invention, the following examples are further provided to illustrate the present invention, but the present invention is not limited to the following examples.
Example 1
A method for producing fermented dry feed which fully plays a role of adsorbing materials comprises the following steps:
(1) preparing a fermentation liquid strain;
(2) preparing adsorption materials, and mixing to prepare a fermentation substrate;
(3) adding the fermentation strain prepared in the step (1) into the fermentation substrate prepared in the step (2), inoculating and mixing, mixing to obtain a wet material with the humidity of 40%, fermenting at 37 ℃ for 96 hours, and inoculating with yeast 6.8 × 106cfu/g, spore bacteria 6.8 × 106cfu/g, lactic acid bacteria 3.4 × 107cfu/g。
(4) And (4) drying the material obtained by fermentation in the step (3) at low temperature to obtain a dry finished product, wherein the moisture of the dry finished product is controlled to be 8-15%.
(5) And (4) crushing the dried finished product prepared in the step (4).
The fermentation strain in the step (1) comprises saccharomycetes, bacillus and lactic acid bacteria, and the ratio of the number of the viable bacteria of the saccharomycetes, the bacillus and the lactic acid bacteria in the fermentation strain is 1:1: 5;
the fermentation substrate in the step (2) comprises 1 part by weight of glucose, 20 parts by weight of montmorillonite, 20 parts by weight of soybean meal, 20 parts by weight of corn flour and 40 parts by weight of bran; the smectite is mixed in at the beginning of the fermentation.
The yeast is brewer's yeast.
The bacillus is bacillus licheniformis.
The lactobacillus is Lactobacillus acidophilus.
The total viable count of the zymophyte liquid prepared in the step (1) is 1.2 × 108cfu/ml。
The culture method of the yeast comprises the following steps:
solid culture medium and culture conditions: 2.0 percent of glucose, 1.0 percent of peptone, 0.5 percent of yeast extract and 2.0 percent of agar, the pH value is 6.8, and the sterilization is carried out for 18 minutes at the temperature of 115 ℃; culturing at 30 deg.c for 24 hr; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: 2.0% of glucose, 1.0% of peptone and 0.5% of yeast extract; sterilizing at 115 deg.C for 18min at an initial pH of 6.8; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 5.0% of culture medium soybean meal, 5% of corn, 2.0% of cane sugar, 1.0% of peptone, 0.5% of yeast extract, 0.1% of monopotassium phosphate, 0.2% of ammonium sulfate, 0.05% of sodium chloride and 0.05% of defoaming agent, sterilizing at the initial pH of 6.8 and 121 ℃ for 30 minutes, cooling to 30 ℃, inoculating a seed tank with seed liquid inoculated in a shake flask according to the proportion of 5% of the inoculum size, and culturing at 30 ℃ for 24 hours. The number of bacteria is: 5.2*107cfu/ml。
The culture method of the bacillus comprises the following steps:
solid culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, agar 2.0%, pH7.0, sterilizing at 115 deg.C for 18 min; culturing at 30 deg.C for 24 h; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, pH7.0, sterilizing at 115 deg.C for 18 min; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 2.0 percent of soybean meal, 2.0 percent of corn, 2.0 percent of cane sugar, 0.4 percent of ammonium sulfate, 0.1 percent of ammonium chloride, 7.0 percent of PH, sterilizing for 30 minutes at 121 ℃, cooling to 30 ℃, inoculating seed liquid in a shake flask into a seeding tank according to the proportion of 5 percent of the inoculation amount, and culturing for 24 hours at 30 ℃. The number of bacteria is: 5.2*107cfu/ml。
The culture method of the lactic acid bacteria comprises the following steps:
2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, pH7.0 and agar 2.0%; 115 ℃ for 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The liquid culture medium and culture conditions of lactobacillus in the eggplant-shaped bottle are as follows: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, wherein the pH value is 7.0, the temperature is 115 ℃ and the time is 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The culture medium and culture conditions for seed tank propagation are as follows: 1.0% of glucose, 0.5% of peptone, 0.5% of yeast powder, 2.0% of corn flour, 2.0% of soybean meal, 0.2kg of ammonium sulfate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.10% of dipotassium hydrogen phosphate and the balance of purified water and pH 6.8; and (3) sterilization conditions: 0.1MPa, 121 ℃ and 20 min; the fermentation culture conditions comprise 0.05Mpa of tank pressure, 50rpm of rotation speed, 33 deg.C of culture temperature, and 48h of culture time. The number of bacteria is: 2.56*108cfu/ml。
Drying and crushing the solid fermentation material, wherein the drying conditions are as follows: drying in a drying furnace at 60 deg.C for 8min at 40 deg.C; the crushing treatment conditions are as follows: the preparation is carried out under sterile and clean conditions, and the granularity of the crushed powder is 20 meshes.
Example 2
A method for producing fermented dry feed which fully plays a role of adsorbing materials comprises the following steps:
(1) preparing a fermentation liquid strain;
(2) preparing adsorption materials, and mixing to prepare a fermentation substrate;
(3) adding the fermentation strain prepared in the step (1) into the fermentation substrate prepared in the step (2), inoculating and mixing, mixing to obtain a wet material with the humidity of 40%, fermenting at 37 ℃ for 96 hours, and inoculating with yeast 1.9 × 107cfu/g, spore bacteria 9.7 × 107cfu/g, lactic acid bacteria 1.9 × 107cfu/g。
(4) And (4) drying the material obtained by fermentation in the step (3) at low temperature to obtain a dry finished product, wherein the moisture of the dry finished product is controlled to be 8-15%.
(5) And (4) crushing the dried finished product prepared in the step (4).
The fermentation strain in the step (1) comprises saccharomycetes, bacillus and lactic acid bacteria, and the ratio of the number of the viable bacteria of the saccharomycetes, the bacillus and the lactic acid bacteria in the fermentation strain is 1:5: 1;
the fermentation substrate in the step (2) comprises 1 part by weight of glucose, 20 parts by weight of montmorillonite, 20 parts by weight of soybean meal, 20 parts by weight of corn flour and 60 parts by weight of bran; the smectite is mixed in at the beginning of the fermentation.
The yeast is candida utilis.
The bacillus is bacillus licheniformis.
The lactobacillus is lactobacillus lactis.
The total viable count of the zymophyte liquid prepared in the step (1) is 3.4 × 108cfu/ml。
The culture method of the yeast comprises the following steps:
solid culture medium and culture conditions: 2.0 percent of glucose, 1.0 percent of peptone, 0.5 percent of yeast extract and 2.0 percent of agar, the pH value is 6.8, and the sterilization is carried out for 18 minutes at the temperature of 115 ℃; culturing at 30 deg.c for 24 hr; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: 2.0% of glucose, 1.0% of peptone and 0.5% of yeast extract; sterilizing at 115 deg.C for 18min at an initial pH of 6.8; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 5.0% of culture medium soybean meal, 5% of corn, 2.0% of cane sugar, 1.0% of peptone, 0.5% of yeast extract, 0.1% of monopotassium phosphate, 0.2% of ammonium sulfate, 0.05% of sodium chloride and 0.05% of defoaming agent, sterilizing at the initial pH of 6.8 and 121 ℃ for 30 minutes, cooling to 30 ℃, inoculating a seed tank with seed liquid in a shake flask according to the proportion of 5% of the inoculation amount, and culturing at 30 ℃ for 24 hours; the number of bacteria is: 4.86*107cfu/ml。
The culture method of the bacillus comprises the following steps:
solid culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, agar 2.0%, pH7.0, sterilizing at 115 deg.C for 18 min; culturing at 30 deg.C for 24 h; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, pH7.0, sterilizing at 115 deg.C for 18 min; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 2.0 percent of soybean meal, 2.0 percent of corn, 2.0 percent of cane sugar, 0.4 percent of ammonium sulfate, 0.1 percent of ammonium chloride, 7.0 percent of PH, sterilizing for 30 minutes at 121 ℃, cooling to 30 ℃, inoculating seed liquid in a shake flask into a seeding tank according to the proportion of 5 percent of the inoculation amount, and culturing for 48 hours at 30 ℃. The number of bacteria is: 2.43*107cfu/ml。
The culture method of the lactic acid bacteria comprises the following steps:
2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, pH7.0 and agar 2.0%; 115 ℃ for 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The liquid culture medium and culture conditions of lactobacillus in the eggplant-shaped bottle are as follows: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, wherein the pH value is 7.0, the temperature is 115 ℃ and the time is 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The culture medium and culture conditions for seed tank propagation are as follows: 1.0% of glucose, 0.5% of peptone, 0.5% of yeast powder, 2.0% of corn flour, 2.0% of soybean meal, 0.2kg of ammonium sulfate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.10% of dipotassium hydrogen phosphate and the balance of purified water and pH 6.8; and (3) sterilization conditions: 0.1MPa, 121 ℃ and 20 min; the fermentation culture conditions comprise 0.05Mpa of tank pressure, 50rpm of rotation speed, 33 deg.C of culture temperature, and 48h of culture time. The number of bacteria is: 4.86*107cfu/ml。
Drying and crushing the solid fermentation material, wherein the drying conditions are as follows: drying in a drying furnace at 60 deg.C for 8min at 40 deg.C; the crushing treatment conditions are as follows: the preparation is carried out under sterile and clean conditions, and the granularity of the crushed powder is 20 meshes.
Example 3
A method for producing fermented dry feed which fully plays a role of adsorbing materials comprises the following steps:
(1) preparing a fermentation liquid strain;
(2) preparing adsorption materials, and mixing to prepare a fermentation substrate;
(3) adding the fermentation strain prepared in the step (1) into the fermentation substrate prepared in the step (2), inoculating and mixing, mixing to obtain a wet material with the humidity of 40%, fermenting at 37 ℃ for 96 hours, and inoculating with the yeast 2.47 × 107cfu/g, spore bacteria 1.24 × 108cfu/g, lactic acid bacteria 1.23 × 108cfu/g。
(4) And (4) drying the material obtained by fermentation in the step (3) at low temperature to obtain a dry finished product, wherein the moisture of the dry finished product is controlled to be 8-15%.
(5) And (4) crushing the dried finished product prepared in the step (4).
The fermentation strain in the step (1) comprises saccharomycetes, bacillus and lactic acid bacteria, and the ratio of the number of the viable bacteria of the saccharomycetes, the bacillus and the lactic acid bacteria in the fermentation strain is 1:1: 5;
the fermentation substrate in the step (2) comprises 1 part by weight of glucose, 20 parts by weight of montmorillonite, 20 parts by weight of soybean meal, 20 parts by weight of corn flour and 40 parts by weight of bran; the smectite is mixed in at the beginning of the fermentation.
The yeast is brewer's yeast.
The bacillus is bacillus subtilis.
The lactobacillus is lactobacillus plantarum.
The total viable count of the zymophyte liquid prepared in the step (1) is 6.8 × 108cfu/ml。
The culture method of the yeast comprises the following steps:
solid culture medium and culture conditions: 2.0 percent of glucose, 1.0 percent of peptone, 0.5 percent of yeast extract and 2.0 percent of agar, the pH value is 6.8, and the sterilization is carried out for 18 minutes at the temperature of 115 ℃; culturing at 30 deg.c for 24 hr; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: 2.0% of glucose, 1.0% of peptone and 0.5% of yeast extract; sterilizing at 115 deg.C for 18min at an initial pH of 6.8; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 5.0% of culture medium soybean meal, 5% of corn, 2.0% of cane sugar and proteinPeptone 1.0%, yeast extract 0.5%, potassium dihydrogen phosphate 0.1%, ammonium sulfate 0.2%, sodium chloride 0.05%, defoaming agent 0.05%, initial pH6.8, sterilizing at 121 deg.C for 30 min, cooling to 30 deg.C, inoculating seed liquid in shake flask into seed tank according to the proportion of 5%, and culturing at 30 deg.C for 24 h. The number of bacteria is: 6.2*107cfu/ml。
The culture method of the bacillus comprises the following steps:
solid culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, agar 2.0%, pH7.0, sterilizing at 115 deg.C for 18 min; culturing at 30 deg.C for 24 h; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, pH7.0, sterilizing at 115 deg.C for 18 min; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 2.0 percent of soybean meal, 2.0 percent of corn, 2.0 percent of cane sugar, 0.4 percent of ammonium sulfate, 0.1 percent of ammonium chloride, 7.0 percent of PH, sterilizing for 30 minutes at 121 ℃, cooling to 30 ℃, inoculating seed liquid in a shake flask into a seeding tank according to the proportion of 5 percent of the inoculation amount, and culturing for 48 hours at 30 ℃. The number of bacteria is: 3.1*108cfu/ml。
The culture method of the lactic acid bacteria comprises the following steps:
2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, pH7.0 and agar 2.0%; 115 ℃ for 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The liquid culture medium and culture conditions of lactobacillus in the eggplant-shaped bottle are as follows: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, wherein the pH value is 7.0, the temperature is 115 ℃ and the time is 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The culture medium and culture conditions for seed tank propagation are as follows: 1.0% of glucose, 0.5% of peptone, 0.5% of yeast powder, 2.0% of corn flour, 2.0% of soybean meal, 0.2kg of ammonium sulfate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.10% of dipotassium hydrogen phosphate and the balancePurified water with pH 6.8; and (3) sterilization conditions: 0.1MPa, 121 ℃ and 20 min; the fermentation culture conditions comprise 0.05Mpa of tank pressure, 50rpm of rotation speed, 33 deg.C of culture temperature, and 48h of culture time. The number of bacteria is: 3.09*108cfu/ml。
Drying and crushing the solid fermentation material, wherein the drying conditions are as follows: drying in a drying furnace at 60 deg.C for 8min at 40 deg.C; the crushing treatment conditions are as follows: the preparation is carried out under sterile and clean conditions, and the granularity of the crushed powder is 20 meshes.
Example 4
A method for producing fermented dry feed which fully plays a role of adsorbing materials comprises the following steps:
(1) preparing a fermentation liquid strain;
(2) preparing adsorption materials, and mixing to prepare a fermentation substrate;
(3) adding the fermentation strain prepared in the step (1) into the fermentation substrate prepared in the step (2), inoculating and mixing, mixing to obtain a wet material with the humidity of 40%, fermenting at 37 ℃ for 96 hours, and inoculating with yeast 6.7 × 107cfu/g, spore bacteria 1.3 × 108cfu/g, lactic acid bacterium 2 × 108cfu/g。
(4) And (4) drying the material obtained by fermentation in the step (3) at low temperature to obtain a dry finished product, wherein the moisture of the dry finished product is controlled to be 8-15%.
(5) And (4) crushing the dried finished product prepared in the step (4).
The fermentation strain in the step (1) comprises saccharomycetes, bacillus and lactic acid bacteria, and the ratio of the number of the viable bacteria of the saccharomycetes, the bacillus and the lactic acid bacteria in the fermentation strain is 1:2: 3;
the fermentation substrate in the step (2) comprises 1 part by weight of glucose, 20 parts by weight of montmorillonite, 20 parts by weight of soybean meal, 20 parts by weight of corn flour and 40 parts by weight of bran; the smectite is mixed in at the beginning of the fermentation.
The yeast is brewer's yeast.
The bacillus is bacillus subtilis.
The lactobacillus is enterococcus faecalis.
The total viable count of the zymophyte liquid prepared in the step (1) is 1.0 × 109cfu/ml。
The culture method of the yeast comprises the following steps:
solid culture medium and culture conditions: 2.0 percent of glucose, 1.0 percent of peptone, 0.5 percent of yeast extract and 2.0 percent of agar, the pH value is 6.8, and the sterilization is carried out for 18 minutes at the temperature of 115 ℃; culturing at 30 deg.c for 24 hr; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: 2.0% of glucose, 1.0% of peptone and 0.5% of yeast extract; sterilizing at 115 deg.C for 18min at an initial pH of 6.8; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 5.0% of culture medium soybean meal, 5% of corn, 2.0% of cane sugar, 1.0% of peptone, 0.5% of yeast extract, 0.1% of monopotassium phosphate, 0.2% of ammonium sulfate, 0.05% of sodium chloride and 0.05% of defoaming agent, sterilizing at the initial pH of 6.8 and 121 ℃ for 30 minutes, cooling to 30 ℃, inoculating a seed tank with seed liquid inoculated in a shake flask according to the proportion of 5% of the inoculum size, and culturing at 30 ℃ for 36 hours. The number of bacteria is: 1.6*108cfu/ml。
The culture method of the bacillus comprises the following steps:
solid culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, agar 2.0%, pH7.0, sterilizing at 115 deg.C for 18 min; culturing at 30 deg.C for 24 h; the inoculation amount is one inoculating loop.
Shake flask seed culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, pH7.0, sterilizing at 115 deg.C for 18 min; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 2.0 percent of soybean meal, 2.0 percent of corn, 2.0 percent of cane sugar, 0.4 percent of ammonium sulfate, 0.1 percent of ammonium chloride, 7.0 percent of PH, sterilizing for 30 minutes at 121 ℃, cooling to 30 ℃, inoculating seed liquid in a shake flask into a seeding tank according to the proportion of 5 percent of the inoculation amount, and culturing for 40 hours at 30 ℃. The number of bacteria is: 3.3*108cfu/ml。
The culture method of the lactic acid bacteria comprises the following steps:
2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, pH7.0 and agar 2.0%; 115 ℃ for 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The liquid culture medium and culture conditions of lactobacillus in the eggplant-shaped bottle are as follows: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, wherein the pH value is 7.0, the temperature is 115 ℃ and the time is 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 h.
The culture medium and culture conditions for seed tank propagation are as follows: 1.0% of glucose, 0.5% of peptone, 0.5% of yeast powder, 2.0% of corn flour, 2.0% of soybean meal, 0.2kg of ammonium sulfate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.10% of dipotassium hydrogen phosphate and the balance of purified water and pH 6.8; and (3) sterilization conditions: 0.1MPa, 121 ℃ and 20 min; the fermentation culture conditions comprise 0.05Mpa of tank pressure, 50rpm of rotation speed, 33 deg.C of culture temperature, and 62h of time. The number of bacteria is: 5.0*108cfu/ml。
Drying and crushing the solid fermentation material, wherein the drying conditions are as follows: drying in a drying furnace at 60 deg.C for 8min at 40 deg.C; the crushing treatment conditions are as follows: the preparation is carried out under sterile and clean conditions, and the granularity of the crushed powder is 20 meshes.
The montmorillonite in examples 1-4 can be replaced or combined with one or more of Maifanitum, Zeolite, Bentonite, Kaolin, Shell powder, sepiolite or Attapulgite.
Example 5
According to the fermentation raw material and the fermentation substrate in the embodiment 1, montmorillonite with different contents is added, and the fermentation substrate in the step (2) comprises 1 part by weight of glucose, 0-50 parts by weight of montmorillonite, 20 parts by weight of soybean meal, 20 parts by weight of corn flour and 0-40 parts by weight of bran; the smectite is mixed in at the beginning of the fermentation. The montmorillonite dosage tested is different in proportion of 0%, 10%, 20%, 30%, 40% and 50% of the total material amount, and the adsorption effect of montmorillonite in different proportions on organic acid produced by fermentation is observed by adopting the scheme of example 4.
The results are shown in Table 1
TABLE 1 Total acid content of fermentation material for different montmorillonite dosages
| Weight ratio of montmorillonite | 0 portion of | 10 portions of | 20 portions of | 30 portions of | 40 portions of | 50 portions of |
| Fermentation drier total acid | 3.5％ | 3.8％ | 4.5％ | 4.1％ | 3.6％ | 3.6％ |
As shown in Table 1, the total acid content of the fermentation material is increased by increasing the adding proportion of the montmorillonite in a certain range, and the total acid retained by the whole feed is the highest at the proportion of 20 percent, which shows that the montmorillonite has better adsorption effect on fermentation metabolites generated in the fermentation process and shows that the adsorption capacity is enhanced along with the increase of the using amount of the montmorillonite; meanwhile, the use ratio of montmorillonite is high, and although montmorillonite also shows the adsorption capacity to metabolites, the fermentation process is influenced to a certain extent, so that the total acid is reduced.
Example 6
According to the fermentation raw material and the fermentation substrate in example 2, different addition times of montmorillonite are adopted in the fermentation process, and the total acid content of the fermented material after fermentation is observed (the total acid is a criterion for product retention of the fermented dry feed, and the total acid is large, which indicates that the loss of the feed in the drying process is small). The addition is started after fermentation, is performed for 48 hours after fermentation and is finished after 96 hours after fermentation, and the addition proportion of the montmorillonite is 20 percent of the total raw material amount in three addition periods. The other protocol was the same as in example 4.
The results are shown in Table 2
TABLE 2 Total acid of fermentation of the dryings with addition of montmorillonite at different fermentation times
| Montmorillonite addition period | Fermentation is started and added | Fermenting for 48 hr, adding | Fermenting for 96 hours and finishing the addition |
| Total acid content of fermentation dry material% | 4.5 | 4.1 | 3.9 |
The results show that the addition of montmorillonite at the beginning of fermentation is most effective, and that the total acid addition is lowest after 48 hours of fermentation.
Example 7
The fermented dry feed product which is obtained in the example 1 and fully exerts the function of adsorbing the montmorillonite is additionally added into the daily ration of the piglet according to the proportion of 2-5% of the daily ration weight, namely 2-5 parts of fermented dry feed is added into 95-98 parts of the daily ration, feeding tests are carried out, namely test groups 1 and 2, the average daily age is 30-32 days old, and the feed for feeding the piglet is twins 'substitute excellent milk', namely the daily ration for comparing all piglets. The results are shown in Table 3:
TABLE 3 influence of the application of a fermented dry feed product that fully exerts the montmorillonite action on the daily gain and feed-to-weight ratio of weaned piglets
As can be seen from table 3: 2-5% of fermented dry feed product which fully exerts the montmorillonite function is added into the daily ration of the weaned piglet, the feeding of a test group is increased compared with that of a control group, and the diarrhea incidence rate is obviously reduced.
Example 8
The fermented dry feed product which gives full play to the montmorillonite function and is obtained in the embodiment 1 is additionally added into the daily ration of the Hailan brown laying hens according to the weight ratio of 2-4%, namely 2-5 parts of fermented dry feed are added into 96-98 parts of daily ration, feeding tests are carried out, namely a test group 1 and a test group 2 are carried out, a control group is 100 parts of daily ration of the Hailan brown laying hens, and the daily ration of the laying hens is as follows: 62% of corn, 3.2% of wheat bran, 31% of soybean meal, 1.3% of calcium hydrophosphate, 1.2% of stone powder, 0.3% of salt, 1% of premix (Zhengda) and test groups. The results are shown in Table 4:
TABLE 4 influence of the use of a fermented dry feed product which fully exerts the montmorillonite action on the daily gain and feed-to-feed ratio of laying hens
As can be seen from table 4: in 420 eggs with the bluish brown shell, the laying rate of a test group is improved by 2.4 percent compared with that of a control group, the daily single-birth rate is improved by 1.44g per egg, and the death and culling rate is reduced by 0.8 percent after a 14-day test period. At the same time, it can be observed that: the eggshell has the advantages of enhanced hardness, brightness, good luster, uniform and consistent color, chicken manure molding, reduced odor and ammonia smell, and obviously improved breeding environment.
Claims (10)
1. A production method of fermented dry feed which fully plays a role of adsorbing materials is characterized by comprising the following steps:
(1) preparing a fermentation liquid strain;
(2) preparing adsorption materials, and mixing to prepare a fermentation substrate;
(3) adding the strain of the fermentation liquid prepared in the step (1) into the fermentation substrate prepared in the step (2) for inoculation and mixing (the weight ratio of the fermentation liquid strain to the sum of the fermentation substrate and the adsorption material is 1:1-4), mixing to obtain a wet material with the humidity of 30-50%, and fermenting at the temperature of 25-40 ℃ for 48-96 hours;
(4) drying the material obtained by fermentation in the step (3) containing the adsorption material at low temperature to obtain a dry finished product, wherein the moisture of the dry finished product is controlled to be 8-15%;
(5) crushing the dried finished product prepared in the step (4);
the fermentation liquid strain in the step (1) comprises saccharomycetes, bacillus and lactic acid bacteria, and the ratio of the viable count of the saccharomycetes, the bacillus and the lactic acid bacteria in the fermentation liquid strain is 1: (1-5) and (1-5);
the fermentation substrate in the step (2) comprises 1-3 parts by weight of glucose, 10-20 parts by weight of soybean meal, 10-20 parts by weight of corn flour and 10-70 parts by weight of bran; 10-70 parts of prepared adsorption material; (preferably 2 parts of glucose, 20 parts of bean pulp, 20 parts of corn flour, 40 parts of bran and 20 parts of adsorption material);
the adsorption materials are as follows: any one or more of montmorillonite, Maifanitum, zeolite, bentonite, kaolin, shell powder, sepiolite and attapulgite.
2. The method for producing fermented dry feed which fully exerts the function of adsorbing materials according to claim 1, wherein the time for adding the adsorbing materials is as follows: the fermentation effect is best when the fermentation substrate and the fermentation seed liquid are mixed within 1-48 hours, the fermentation effect is next to be added within more than 48-96 hours after the fermentation is started, and the fermentation substrate and the fermentation seed liquid are mixed again within more than 96 hours after the fermentation is started-before the fermentation is finished or before the drying.
3. The method for producing the fermented dry feed which can sufficiently absorb materials according to claim 1, wherein the bacillus is one or a mixture of more than two of bacillus subtilis and bacillus licheniformis in any proportion;
the yeast is one or a mixture of more than two of candida utilis and saccharomyces cerevisiae in any proportion;
the lactobacillus is one or more than two mixed bacteria in any proportion of lactobacillus acidophilus, lactobacillus casei, lactobacillus delbrueckii subsp lactis, lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, bifidobacterium bifidum, enterococcus faecalis, enterococcus faecium, enterococcus lactis, lactobacillus fermentum and lactobacillus bulgaricus.
4. The method for producing fermented dry feed with full play of material adsorption according to claim 1, wherein the fermentation strains in step (1) are separately added into the fermentation substrate prepared in step (2) for inoculation and mixing or the fermentation strains in step (1) are mixed and then added into the fermentation substrate prepared in step (2).
5. The method for producing fermented dry feed which fully exerts the function of adsorbing materials according to claim 1, wherein the total viable count of the zymophyte liquid prepared in the step (1) is 1.0 × 108～1.0×109One/ml.
6. The method for producing fermented dry feed which fully exerts the function of adsorbing materials according to claim 1, wherein the step (1) of preparing the fermentation strain specifically comprises the following steps:
the fermentation liquid strain is yeast, bacillus, lactobacillus;
the culture method of the yeast comprises the following steps:
slant culture medium and culture conditions: 2.0 percent of glucose, 1.0 percent of peptone, 0.5 percent of yeast extract and 2.0 percent of agar, the pH value is 6.8, and the sterilization is carried out for 18 minutes at the temperature of 115 ℃; culturing at 30 deg.c for 24 hr;
shake flask seed culture medium and culture conditions: 2.0% of glucose, 1.0% of peptone and 0.5% of yeast extract; sterilizing at 115 deg.C for 18min at an initial pH of 6.8; inoculating the culture dish strain into a shake flask, placing into a constant temperature shaking table at 30 ℃, and carrying out shaking culture at 200r/min for 24 h;
the seed tank propagation culture medium comprises 5.0% of culture medium soybean meal, 5% of corn, 2.0% of cane sugar, 1.0% of peptone, 0.5% of yeast extract, 0.1% of potassium dihydrogen phosphate, 0.2% of ammonium sulfate, 0.05% of sodium chloride, 0.05% of defoaming agent, sterilizing at the initial pH of 6.8 and 121 ℃ for 30 minutes, cooling to 30 ℃, inoculating the seed liquid in the shake flask into the seed tank according to the proportion of 5% of the inoculation amount, wherein the pressure of the seed tank is 0.05Mpa, the rotating speed is 50rpm, the culture time at 30 ℃ is 24 hours, and the obtained viable count range is 1.0 × 107～1.0×109One/ml.
7. The method for producing fermented dry feed which sufficiently exerts the function of adsorbing materials according to claim 1, wherein the method for culturing the bacillus comprises:
slant culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, agar 2.0%, pH7.0, sterilizing at 115 deg.C for 18 min; culturing at 30 deg.C for 24 h;
shake flask seed culture medium and culture conditions: peptone 1.0%, yeast extract 0.5%, NaCl1.0%, pH7.0, placing in 30 deg.C constant temperature shaking table, and shake culturing at 200r/min for 24 h;
the culture medium and culture conditions for seed tank propagation are as follows: 2.0% of soybean meal, 2.0% of corn, 2.0% of cane sugar, 0.4% of ammonium sulfate, 0.1% of ammonium chloride, 7.0 of PH, sterilizing at 121 ℃ for 30 minutes, cooling to 30 ℃, inoculating seed liquid in a shake flask into a seed tank according to the proportion of 5% of the inoculum size, wherein the tank pressure is 0.05Mpa, the rotating speed is 50rpm, the culture time is 24 hours at 30 ℃, and the number of obtained viable bacteria isRange 1.0 × 107～1.0×109One/ml.
8. The method for producing fermented dry feed which sufficiently exerts the function of adsorbing materials according to claim 1, wherein the method for culturing the lactic acid bacteria comprises:
2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, pH7.0 and agar 2.0%; 115 ℃ for 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 hours;
the liquid culture medium and culture conditions of lactobacillus in the eggplant-shaped bottle are as follows: 2.0% of glucose, 1.0% of peptone, 0.6% of beef extract, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate and the balance of purified water, wherein the pH value is 7.0, the temperature is 115 ℃ and the time is 18 min; the culture temperature is as follows: the temperature is 26-32 ℃, and the time is 48-72 hours;
the seed tank propagation medium comprises 1.0% of glucose, 0.5% of peptone, 0.5% of yeast powder, 2.0% of corn flour, 2.0% of soybean meal, 0.2kg of ammonium sulfate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.10% of dipotassium hydrogen phosphate, the balance of purified water and pH6.8, and is prepared by sterilizing at 0.1MPa, 121 ℃ and 20min, fermenting at 0.05MPa and 33 ℃ for 48h to obtain viable bacteria with the viable bacteria number range of 1.0 × 107～1.0×109One/ml.
9. The method for producing fermented dry feed which fully exerts the function of adsorbing materials according to claim 1, wherein the step (4) is to carry out low-temperature drying on the material fermented in the step (3) containing the adsorbing materials, and the drying conditions are as follows: and drying by adopting a drying furnace, wherein the temperature in the drying furnace is 60-80 ℃, the outlet temperature of the drying furnace is 38-42 ℃, and the time is 8-10 min.
10. The method for producing fermented dry feed which can sufficiently absorb materials according to claim 1, wherein the particle size of the crushed material in the step (5) is 20-40 meshes.
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