KR102472487B1 - Cosmetic composition comprising natural extract mixture - Google Patents

Abstract

The present invention relates to a whitening functional cosmetic composition comprising a natural extract mixture having a whitening effect including camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract.

Description

Whitening functional cosmetic composition comprising natural extract mixture {Cosmetic composition comprising natural extract mixture}

The present invention relates to a cosmetic composition, and more particularly, to a whitening functional composition comprising a natural extract mixture having a whitening effect including camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract. It relates to a cosmetic composition.

In general, the skin is in charge of the primary function of preventing harm from various external factors that impede the homeostasis of our body through basic metabolic processes. In other words, as the skin is located at the outermost part of the body, it performs various roles such as protection through physical, chemical, and biological barrier functions, temperature regulation, sensation, secretion and excretion, absorption, storage, and regeneration. However, it is most susceptible to damage when subjected to sustained external stress.

Today, Asian women prefer white and clean skin like white jade and consider it an important standard of beauty. Therefore, development of whitening agents for treatment of abnormal skin pigmentation and satisfaction of beauty needs is being actively carried out. For example, skin blackening Substances having the effect of inhibiting tyrosinase, an enzyme involved in the process, or inhibiting melanin polymerization are used in combination with cosmetics. Representative materials used for this purpose include hydroquinone, ascorbic acid, kojic acid, thiol-based compounds such as glutathione and cysteine, and alpha hydroxy acids.

However, it is known that most of these components themselves are unstable, have a weak whitening effect, and also cause irritation to the skin. Therefore, there is an increasing movement to obtain a whitening effect using natural products.

As a conventional document related to such a whitening effect, Korean Patent Publication No. 10-2016-0056268 (published on May 19, 2016), "for skin whitening or anti-inflammatory containing guava extract or a fraction thereof as an active ingredient" Composition" has been posted, which relates to a composition for skin whitening comprising a guava extract or a fraction thereof as an active ingredient.

However, the conventional "composition for skin whitening or anti-inflammatory containing a guava extract or a fraction thereof as an active ingredient" has insignificant effect, so there is a growing need for a composition capable of improving this.

Korean Patent Publication No. 10-2016-0056268 (published on May 19, 2016), "Composition for skin whitening or anti-inflammatory containing guava extract or its fraction as an active ingredient"

In order to solve the above problems, the present invention is a whitening functional cosmetic comprising a natural extract mixture having a whitening effect including camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract. It is an object to provide a composition.

In order to achieve the above object, the present invention is a whitening functional cosmetic composition comprising a natural extract mixture comprising camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract. provides

In addition, the camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract are mixed in a weight ratio of 0.001 to 40% by weight, respectively.

In addition, the camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract are obtained by mixing black camu fruit, guava fruit, vitamin tree fruit, kakadu plum, and avocado fruit with water and 1 to 4 carbon atoms. It is characterized by extraction with at least one extraction solvent selected from the group consisting of alcohol, butylene glycol and hexanediol.

In addition, it is characterized in that it has a whitening effect, including the natural extract mixture.

In addition, using the natural extract mixture, skin, lotion, cream, foundation, essence, gel, pack, sunscreen cream, water-soluble liquid, oil-in-water (O / W) type cosmetics, water-in-oil (W / O) type It is characterized in that it is formulated into any one of cosmetics and silicon-in-water (W / Si) type cosmetics, solutions, suspensions, emulsions, pastes, powders, soaps, surfactant-containing cleansing, oils, sprays or mask sheets .

The whitening functional cosmetic composition comprising the natural extract mixture according to the present invention includes camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract, and has a whitening effect.

1 is a flow chart showing a manufacturing process of a whitening functional cosmetic composition comprising a natural extract mixture according to the present invention.
2 to 4 are graphs showing the whitening effect test results of the compositions of Examples 1 to 2 and Comparative Examples 1 to 10 according to the present invention.

The detailed description of the present invention below refers to the accompanying drawings shown as examples of embodiments in which the present invention can be practiced. These embodiments are described in detail so that those skilled in the art will be able to practice the present invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in one embodiment in another embodiment without departing from the spirit and scope of the invention. Additionally, it should be understood that the location or arrangement of individual components within each described embodiment may be changed without departing from the spirit and scope of the invention.

Accordingly, the detailed description set forth below is not to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all equivalents as claimed by those claims. Like reference numbers in the drawings indicate the same or similar function throughout the various aspects.

The terms used in the present invention have been selected from general terms that are currently widely used as much as possible while considering the functions in the present invention, but these may vary depending on the intention of a person skilled in the art or precedent, the emergence of new technologies, and the like. In addition, in a specific case, there is also a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, not simply the name of the term.

In the present invention, when a part "includes" a certain component, it means that it may further include other components, not excluding other components, unless otherwise stated.

The whitening functional cosmetic composition comprising the natural extract mixture according to the present invention is characterized in that it includes camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract.

The camu camu fruit extract refers to an extract obtained from the fruit of camu camu. The camu camu ( Myrciaria Dubia ) is a fruit of a tree that grows in the Amazon rain forest region of Brazil and Peru, and mainly lives in the Amazon River wetland. It is named after Kamukamura. It has a very high vitamin content, containing 43 times more vitamin C than lemons and 50 times more than oranges.

The guava fruit extract refers to an extract obtained from the fruit of guava. The guava (Plsidium guajava) is a shrub of the dicotyledonous myrtle myrtle family, and has many branches with a height of 3 to 7 m. Leaves are opposite, leathery, egg-shaped or long oval. The leaves emit a strong scent when pressed. The flower is about 3 cm in diameter and has 4 petals. It hangs on the leaf axil and usually blooms one by one, but sometimes 2-3 white flowers bloom.

The vitamin tree fruit extract refers to an extract obtained from the fruit of a vitamin tree. The vitamin tree ( Hippophae rhamnoides ) is a deciduous shrub covered with long spines of the oleaceae family. Dorsal root Yellow-orange berries are 6 to 9 millimeters in size, juicy and high in oil. Rich in vitamins C and E, carotenoids and flavonoids. Makes juices, sauces, and liquors. It also produces oil that has healing effects such as cold, fever, and fatigue. It is native to North Asia and Europe.

The kakadu plum extract refers to an extract obtained from kakadu plum. The Kakadu Plum ( Terminalia Ferdinandiana ) is a fruit named after Kakadu National Park in Australia and is called a Western plum and contains 100 times more vitamin C than an orange. It grows in the barren environment of the tropical savannah in the northern part of Australia and is a rare fruit that must be harvested by hand in the wild because farming is impossible. It is green and has hard seeds inside. Known to grow wild in Australia's Kakadu National Park, it is called the Kakadu Plum.

The avocado fruit extract refers to an extract obtained from the fruit of avocado. The avocado ( Persea Gratissima ) is a name derived from the word 'testicle' in the language of the Nahuatl in southern Mexico, and was named after the shape of the fruit resembles the testicles of an animal. Alligator pair in English means that the skin of the fruit is a thick pear like the skin of a crocodile. It is native to Central America, such as Mexico, Guatemala, and Costa Rica, island countries in the Caribbean, and South America north of Peru. The reason why the flesh is soft like butter is because it has a high fat content, and the flesh of a fully ripened fruit can be easily dug out with a spoon. Hard fruit will soon become soft if stored at room temperature for 2-3 days for post-ripening.

The whitening functional cosmetic composition containing the natural extract mixture according to the present invention includes an extract mixture obtained by mixing camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract.

The camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract are prepared by mixing black camu fruit, guava fruit, vitamin tree fruit, kakadu plum, and avocado fruit with water and alcohol having 1 to 4 carbon atoms. , It is appropriate to extract with at least one extraction solvent selected from the group consisting of butylene glycol and hexanediol.

Most preferably, the extraction solvent is a mixture of butylene glycol and hexanediol.

The camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract are prepared by mixing camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum, and avocado fruit at a temperature of 40 to 150 ° C. It is preferred to extract for ~24 hours.

The whitening functional cosmetic composition comprising a natural extract mixture according to the present invention mixes camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract, and in the mixing process of each extract, Interactions between key ingredients have unexpected effects.

On the other hand, in the whitening functional cosmetic composition containing the natural extract mixture according to the present invention, camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract are mixed in a weight ratio of 0.001 to 40% by weight, respectively. it is desirable to be

Most preferably, the natural extract mixture according to the present invention comprises 20.0% by weight of camu camu fruit extract, 20.0% by weight of guava fruit extract, 20.0% by weight of vitamin tree fruit extract, 20.0% by weight of kakadu plum extract and 20.0% by weight of avocado fruit extract. It would be preferable to mix

On the other hand, the natural extraction mixture may undergo a maturation period for 1 to 7 days at a temperature of 10 to 40 ℃. When the natural extraction mixture is aged, the effect of the whitening functional cosmetic composition containing the natural extraction mixture of the present invention is increased.

Hereinafter, the manufacturing process of the whitening functional cosmetic composition containing the natural extract mixture according to the present invention will be briefly described.

The manufacturing process of the whitening functional cosmetic composition containing the natural extract mixture according to the present invention may include a washing and drying step (S10), an extraction step (S20), a mixing step (S30), and an aging step (S40).

In the washing and drying step (S10), camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum, and avocado fruit are prepared and cut into 1 to 1 cm slices, followed by washing and drying.

Then, in the extraction step (S20), water, alcohol having 1 to 4 carbon atoms, acetone, butyl are added to the washed camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum and avocado fruit washed in the washing and drying step (S10) At least one extraction solvent selected from the group consisting of len glycol and hexanediol was added and extracted at a temperature of 40 to 150 ° C for 8 to 24 hours to obtain camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, and kakadu plum Extract and avocado fruit extract are prepared.

Next, in the mixing step (S30), the camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract prepared in the extraction step (S20) were mixed in a weight ratio of 0.001 to 40% by weight, respectively. by mixing to prepare an extract mixture.

Finally, in the aging step (S40), the extraction mixture prepared in the mixing step (S30) is aged for 1 to 7 days at a temperature of 10 to 40 ° C.

The whitening functional cosmetic composition containing the natural extract mixture completed through the above process has a whitening effect.

More specifically, the whitening functional cosmetic composition comprising the natural extract mixture according to the present invention is a natural extract obtained by mixing camu camu fruit peel extract, guava fruit flower extract, vitamin tree fruit extract, kakadu plum fruit extract, and avocado fruit fruit extract. Including the mixture, it has the effect of increasing the whitening effect compared to the single extract in which each raw material is separately extracted.

On the other hand, the whitening functional cosmetic composition containing the natural extract mixture according to the present invention is skin, lotion, cream, foundation, essence, gel, pack, sunscreen cream, water-soluble liquid, oil-in-water (O / W) type cosmetics, oil Heavy water (W/O) type cosmetics and silicon heavy water (W/Si) type cosmetics, solutions, suspensions, emulsions, pastes, powders, soaps, surfactant-containing cleansing, oils, sprays, or mask sheets. can be formulated.

Hereinafter, the effect of the whitening functional cosmetic composition containing the natural extract mixture according to the present invention will be described in detail through the following Examples, Comparative Examples, and Experimental Examples.

Example 1. Camu camu fruit extract , Guava Fruit Extract , vitamin tree fruit extract , Preparation of extract mixture by mixing kakadu plum extract and avocado fruit extract

An extract mixture was prepared by mixing camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract according to the following preparation method.

(S10): Camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum and avocado fruit were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum and avocado fruit, respectively, at a temperature of 60 ° C. for 12 hours After extraction, camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract were prepared.

(S30): An extract mixture was prepared by mixing 20.0% by weight of each of the prepared camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract.

(S40): The prepared extraction mixture was aged for 3 days at a temperature of 25°C.

Example 2. Camu Camu Fruit Extract , Guava Fruit Extract , Vitamin Tree Fruit Extract , Preparation of an extract mixture mixing kakadu plum extract and avocado fruit extract but excluding aging process

According to the following manufacturing method, an extract mixture was prepared by mixing camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract, but excluding the aging process.

(S10): Camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum and avocado fruit were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum and avocado fruit, respectively, at a temperature of 60 ° C. for 12 hours After extraction, extracts of camu camu fruit, guava fruit, vitamin tree fruit, kakadu plum, and avocado fruit were prepared.

(S30): An extract mixture was prepared by mixing 20.0% by weight of each of the prepared camu camu fruit peel extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract.

comparative example One. Camu Camu Fruit Preparation of single extract

According to the following manufacturing process, the composition of Comparative Example 1 was prepared to contain only the extract of black camu camu fruit.

Washing and drying step (S10): After preparing the camu camu fruit and cutting it into small-sized pieces, it was washed and dried in a well-ventilated shade.

Preparation step (S20): A raw material of camu camu fruit alone was prepared so that 100% by weight of camu camu fruit was included.

Extraction step (S30): An extraction solvent mixed with butylene glycol and hexanediol was added to the single raw material of camu camu fruit prepared in the mixing step (S20) and extracted at a temperature of 60 ° C for 12 hours to obtain a single extract of camu camu fruit was manufactured.

Aging step (S40): The single extract of camu camu fruit prepared in the extraction step (S30) was aged at a temperature of 25°C for 3 days.

comparative example 2. Guava fruit Preparation of single extract

According to the following manufacturing process, the composition of Comparative Example 2 was prepared to contain only the extract of guava fruit alone.

Washing and drying step (S10): Prepare guava fruits and cut them into small-sized pieces, then wash and dry in a well-ventilated shade.

Preparation step (S20): A raw material of guava fruit alone was prepared so that 100% by weight of guava fruit was included.

Extraction step (S30): Adding an extraction solvent mixed with butylene glycol and hexanediol to the raw material of the guava fruit alone prepared in the mixing step (S20) and extracting at a temperature of 60 ° C. for 12 hours to prepare a single guava fruit extract did

Aging step (S40): The guava fruit alone extract prepared in the extraction step (S30) was aged at a temperature of 25 ° C. for 3 days.

comparative example 3. vitamin tree fruit Preparation of single extract

According to the following manufacturing process, the composition of Comparative Example 3 was prepared to contain only the vitamin tree fruit extract alone.

Washing and drying step (S10): After preparing the vitamin tree fruit and cutting it into small-sized pieces, it was washed and dried in a well-ventilated shade.

Preparation step (S20): A raw material of vitamin tree fruit was prepared so that 100% by weight of vitamin tree fruit was included.

Extraction step (S30): An extraction solvent mixed with butylene glycol and hexanediol was added to the raw material of the vitamin tree fruit alone prepared in the mixing step (S20), followed by extraction at a temperature of 60 ° C. for 12 hours to obtain a single vitamin tree fruit extract. was manufactured.

Aging step (S40): The vitamin tree fruit alone extract prepared in the extraction step (S30) was aged at a temperature of 25 ° C. for 3 days.

comparative example 4. kakadu plum Preparation of single extract

According to the following manufacturing process, the composition of Comparative Example 4 was prepared to contain only the kakadu plum extract alone.

Washing and drying step (S10): Kakadu plum was prepared and cut into small-sized pieces, then washed and dried in a well-ventilated shade.

Preparation step (S20): Kakadu plum alone raw material was prepared so that 100% by weight of kakadu plum was included.

Extraction step (S30): Kakadu plum single extract prepared in the mixing step (S20) by adding an extraction solvent mixed with butylene glycol and hexanediol to the raw material of kakadu plum and extracting at 60 ° C for 12 hours was manufactured.

Aging step (S40): The kakadu plum alone extract prepared in the extraction step (S30) was aged at a temperature of 25 ° C for 3 days.

comparative example 5. Preparation of avocado fruit extract alone

According to the following manufacturing process, the composition of Comparative Example 5 was prepared to include only the avocado fruit extract.

Washing and drying step (S10): The avocado fruit was prepared and cut into small-sized pieces, then washed and dried in a well-ventilated shade.

Preparation step (S20): A single raw material of avocado fruit was prepared so that 100% by weight of avocado fruit was included.

Extraction step (S30): An extraction solvent mixed with butylene glycol and hexanediol was added to the raw material of the avocado fruit alone prepared in the mixing step (S20), and extraction was performed at a temperature of 60 ° C for 12 hours to obtain a single extract of the avocado fruit. manufactured.

Aging step (S40): The avocado fruit alone extract prepared in the extraction step (S30) was aged at a temperature of 25 ° C. for 3 days.

comparative example 6. Camu camu fruit extract Preparation of Excluded Extraction Mixture

According to the following manufacturing method, an extract mixture excluding some components was prepared.

(S10): Guava fruit, vitamin tree fruit, kakadu plum and avocado fruit were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried guava fruit, vitamin tree fruit, kakadu plum and avocado fruit, respectively, and extracted at a temperature of 60 ° C. for 12 hours, guava Fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract were prepared.

(S30): An extract mixture was prepared by mixing 25.0% by weight of each of the prepared guava fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract.

(S40): The prepared extraction mixture was aged for 3 days at a temperature of 25°C.

comparative example 7. Guava fruit extract Preparation of Excluded Extraction Mixture

According to the following manufacturing method, an extract mixture excluding some components was prepared.

(S10): Camu camu fruit, vitamin tree fruit, kakadu plum and avocado fruit were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried camu camu fruit, vitamin tree fruit, kakadu plum and avocado fruit, respectively, and extracted at a temperature of 60 ° C. for 12 hours, Camu camu fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract were prepared.

(S30): An extract mixture was prepared by mixing 25.0% by weight of each of the prepared camu camu fruit extract, vitamin tree fruit extract, kakadu plum extract, and avocado fruit extract.

(S40): The prepared extraction mixture was aged for 3 days at a temperature of 25°C.

comparative example 8. Vitamin tree fruit extract Preparation of Excluded Extraction Mixture

According to the following manufacturing method, an extract mixture excluding some components was prepared.

(S10): Camu camu fruit, guava fruit, kakadu plum and avocado fruit were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried camu camu fruit, guava fruit, kakadu plum, and avocado fruit, respectively, and extracted at a temperature of 60 ° C. for 12 hours. Blackberry fruit extract, guava fruit extract, kakadu plum extract and avocado fruit extract were prepared.

(S30): An extract mixture was prepared by mixing 25.0% by weight of each of the prepared camu camu fruit extract, guava fruit extract, kakadu plum extract, and avocado fruit extract.

(S40): The prepared extraction mixture was aged for 3 days at a temperature of 25°C.

comparative example 9. kakadu plum extract Preparation of Excluded Extraction Mixture

According to the following manufacturing method, an extract mixture excluding some components was prepared.

(S10): Camu camu fruit, guava fruit, vitamin tree fruit, and avocado fruit were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried camu camu fruit, guava fruit, vitamin tree fruit and avocado fruit, respectively, and extracted at a temperature of 60 ° C. for 12 hours, Blackberry fruit extract, guava fruit extract, vitamin tree fruit extract and avocado fruit extract were prepared.

(S30): An extract mixture was prepared by mixing 25.0% by weight of each of the prepared camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, and avocado fruit extract.

(S40): The prepared extraction mixture was aged for 3 days at a temperature of 25°C.

comparative example 10. Preparation of extract mixture excluding avocado fruit extract

According to the following manufacturing method, an extract mixture excluding some components was prepared.

(S10): Camu camu fruit, guava fruit, vitamin tree fruit and kakadu plum were prepared and cut into small-sized slices, washed and dried.

(S20): An extraction solvent mixed with butylene glycol and hexanediol was added to the cut, washed and dried camu camu fruit, guava fruit, vitamin tree fruit and kakadu plum, respectively, and extracted at a temperature of 60 ° C. for 12 hours, Camu camu fruit extract, guava fruit extract, vitamin tree fruit extract and kakadu plum extract were prepared.

(S30): An extract mixture was prepared by mixing 25.0% by weight of each of the prepared camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, and kakadu plum extract.

(S40): The prepared extraction mixture was aged for 3 days at a temperature of 25°C.

Experimental example One. Example 1 to 2 and comparative example for compositions from 1 to 10 tyrosinase ) check inhibitory activity

In this experimental example, in order to confirm the whitening activity of the compositions of Examples 1 to 2 and Comparative Examples 1 to 10, a tyrosinase inhibition test was performed as follows by modifying the method notified by the Korea Food and Drug Administration.

40 μl of 150 units/ml mushroom tyrosinase and 60 μl of 1.5 mM L-tyrosine were added to 50 μl of the phosphorylation buffer solution containing 200 μg/mL of the compositions of Examples 1 to 2 and Comparative Examples 1 to 10 and mixed well. , The reaction was performed at 37° C. for 10 minutes, and the absorbance of the reaction product, dopachrome, was measured at 475 nm using a microplate reader (Molecular Devices). After the same experiment as above was performed using arbutin as a positive control, the degree of inhibition of tyrosinase activity of the compositions of Examples 1 to 2 and Comparative Examples 1 to 10 was compared.

Tyrosinase activity inhibition rate (%) Example 1 47.8 Example 2 45.5 Comparative Example 1 27.5 Comparative Example 2 26.6 Comparative Example 3 28.2 Comparative Example 4 26.7 Comparative Example 5 25.4 Comparative Example 6 35.3 Comparative Example 7 37.9 Comparative Example 8 34.2 Comparative Example 9 34.1 Comparative Example 10 33.3 Arbutin 50.4

As shown in Table 1, it was confirmed that the compositions of Examples 1 to 2 and Comparative Examples 1 to 10 had a tyrosinase inhibitory effect. In particular, it was confirmed that Example 1 had a higher inhibitory effect than Example 2. Therefore, it was inferred that an unexpected effect appeared in the process of mixing and aging each extract.

Experimental example 2. Example 1 to 2 and comparative example Confirmation of the melanin production inhibitory effect of the compositions of 1 to 10

The compositions of Examples 1 to 2 and Comparative Examples 1 to 10 and hydroquinone aqueous solution were added to the culture medium of B-16 mouse melanoma cells to test the whitening effect at the cellular level (Lotan R. , Lotan D. Cancer Res. 40:3345-3350, 1980). The compositions of Examples 1 to 2 and Comparative Examples 1 to 10 were added to the culture medium of B-16 melanoma cells to a final concentration of 0.005%, and the concentration of the hydroquinone aqueous solution to 0.0001%, respectively, and cultured for 3 days. After that, the cells were treated with trypsin, detached from the culture vessel, centrifuged, and then melanin was extracted.

Here, 1 ml of sodium hydroxide solution (1N concentration) was added and boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer, and the amount of melanin produced was expressed as absorbance per unit cell number (10 ⁶ cell). In addition, the relative amount of melanin production with respect to the control group was calculated as an inhibition rate (%), and the results are shown in Table 2 below.

Amount of melanin produced Inhibition rate (%) control group 0.050±0.003 - Example 1 0.027±0.002 46% Example 2 0.029±0.002 42% Comparative Example 1 0.045±0.003 10% Comparative Example 2 0.044±0.003 12% Comparative Example 3 0.045±0.002 10% Comparative Example 4 0.044±0.002 12% Comparative Example 5 0.045±0.003 10% Comparative Example 6 0.038±0.003 24% Comparative Example 7 0.037±0.002 26% Comparative Example 8 0.039±0.002 22% Comparative Example 9 0.038±0.002 24% Comparative Example 10 0.037±0.003 26% hydroquinone 0.025±0.002 50%

Referring to Table 2, when compared to the compositions of Comparative Examples 1 to 10, the compositions of Examples 1 to 2 not only showed a very strong ability to inhibit melanin production against cultured rat melanoma cells, but were not purified at all. Considering that it is an untreated extract, it can be seen that the effect is not inferior even when compared to hydroquinone, a known whitening substance.

Experimental example 3. Example 1 to 2 and comparative example 1 to 10 Whitening effect on the composition Confirm

For 20 healthy women, an opaque tape with 6 holes of 2 cm in diameter was attached to each subject's upper arm, and 1.5 to 2 times the minimum erythema dose of each subject was irradiated. to induce darkening of the skin, and after two months of applying the above test substances, the contrast of the skin was measured using a color difference meter. Next, the women were given the compositions of Examples 1 to 2 and Comparative Examples 1 to 10 twice a day, morning and evening. Arbutin was applied as a control.

The determination of the effect was determined by obtaining the ㅿL* value (difference in skin color) of the following formula representing the lightness and darkness of the skin. For reference, Korean skin color that is not artificially tanned generally shows a value of 50-70. Minolta CR 300 was used as a measuring device, and the results are shown in Table 3 below.

ㅿL* = ㅿL* value on the last day of application of the complex extract - ㅿL* value before application of the complex extract

Whitening effect measurement result (ΔL') Example 1 3.15 Example 2 3.06 Comparative Example 1 1.84 Comparative Example 2 1.78 Comparative Example 3 1.74 Comparative Example 4 1.83 Comparative Example 5 1.74 Comparative Example 6 2.46 Comparative Example 7 2.54 Comparative Example 8 2.53 Comparative Example 9 2.42 Comparative Example 10 2.37 Arbutin 3.25

As can be seen in Table 3, in the case of fermented extracts according to Examples 1 to 2 of the present invention, significantly improved whitening effect can be obtained compared to Comparative Examples 1 to 10, and even compared to arbutin, a well-known and widely known whitening material, it is equally similar. It was confirmed that a range-level whitening effect could be achieved.

conclusion.

Through the above Examples, Comparative Examples and Experimental Examples, the effect of the whitening functional cosmetic composition containing the natural extract mixture according to the present invention was investigated.

Through Experimental Examples 1 to 3, it was confirmed that the whitening functional cosmetic composition containing the natural extract mixture according to the present invention had a skin whitening effect, and when the raw materials according to the present invention were mixed, compared to the composition lacking a part, it was superior It was confirmed that it had an effect. From this, it was inferred that an unexpected effect appeared by mixing the raw material according to the present invention.

Accordingly, the present invention states that a whitening functional cosmetic composition containing a natural extract mixture having a whitening effect including camu camu fruit extract, guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract has been developed. do.

Although the present invention has been described with the accompanying drawings, this is only one example of various embodiments including the gist of the present invention, and is intended to be easily practiced by those skilled in the art. As an object, it is clear that the present invention is not limited to the embodiments described above. Therefore, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent range by change, substitution, substitution, etc. within the scope of the present invention do not depart from the scope of the present invention. will be included In addition, it is clear that some configurations in the drawings are provided exaggerated or reduced than the actual configuration to more clearly explain the configuration.

(S10): washing and drying step
(S20): Extraction step
(S30): mixing step
(S40): Aging step

Claims (5)

Camu camu fruit extract obtained by adding an extraction solvent mixed with butylene glycol and hexanediol to camu camu fruit, guava fruit, vitamin tree, kakadu plum, and avocado fruit, respectively, and extracting at 40 ~ 150 ℃ for 8 ~ 24 hours , A whitening functional cosmetic composition comprising a natural extract mixture comprising a natural extract mixture obtained by mixing guava fruit extract, vitamin tree fruit extract, kakadu plum extract and avocado fruit extract in a weight ratio of 20.0% by weight, respectively.
delete delete According to claim 1,
A whitening functional cosmetic composition comprising a natural extract mixture, characterized in that it has a whitening effect, including the natural extract mixture.
Skin, lotion, cream, foundation, essence, gel, pack, sunscreen cream, water-soluble liquid, oil-in-water (O/W) type cosmetics, water-in-oil (W/O) type using the natural extract mixture of claim 1 Characterized in that it is formulated into any one of cosmetics and silicon-in-water (W / Si) type cosmetics, solutions, suspensions, emulsions, pastes, powders, soaps, surfactant-containing cleansing, oils, sprays or mask sheets cosmetic composition.

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