KR101757457B1 - Cosmetic composition containing natural complex extract for anti-thermal skin aging and skin soothing - Google Patents

Abstract

An embodiment of the present invention relates to a method for producing a plant extract comprising: And a cosmetic composition comprising, as an active ingredient, a complex extract comprising a safflower extract, a peppermint extract, and a gossam extract.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a cosmetic composition for prevention of heat aging and skin soiling which comprises a natural combination extract,

More particularly, the present invention relates to a cosmetic composition for prevention of heat aging and skin soothing, and more particularly to a cosmetic composition for preventing skin aging and skin soiling, To an anti-aging cosmetic composition excellent in collagen synthesis, MMP-1 inhibition, and skin soothing effect.

Cosmetics can be roughly divided into basic cosmetics for supplying nutrition to the skin and color cosmetics mixed with harmless raw materials to express the outline in a unique way. In particular, basic cosmetics include lotion, lotion, skin, and lotion, and most women and men use basic cosmetics. These basic cosmetics remove the keratin and wastes of the skin to clean the skin, control the oil and water in a balanced manner, calm the skin damaged by cleansing, and form a thin protective film to prevent skin troubles.

Also, in recent years, as the income increases, the level of living has been improved and the investment in cosmetics has been increasing to enhance the image of the self. In addition, as the elderly population increases, interest and demand for functional cosmetics related to anti-aging, wrinkle improvement, whitening, and ultraviolet rays are increasing. However, since such basic cosmetic or functional cosmetic compositions are mostly made of chemical substances, skin troubles often occur when they are applied to sensitive skin.

On the other hand, active oxygen introduced from the outside of the living body or generated in the living body causes various problems such as promoting the aging of the living body or causing cancer. Therefore, many studies and studies have been conducted on antioxidants that inhibit oxidation by active oxygen. Conventionally, there have been many attempts to obtain an anti-aging effect of skin by adding an antioxidant such as superoxide dismutase (SOD), vitamin E derivative, ascorbic acid and its derivatives, and flavonoid to the cosmetic, The use of cosmetic preparations is limited because of problems in stability.

Inflammation is a series of defenses aimed at minimizing the response of a cell or tissue to any cause and restoring the damaged area to its original state. It causes nerve and blood vessels, lymphatic vessels, humoral responses, and cellular reactions resulting in pain, Swelling, redness, fever, etc., resulting in dysfunction. The causes of inflammation are physical factors caused by trauma, bronze, burn, radioactivity, and chemical factors caused by chemical substances such as acid, and immunological factors caused by antibody reaction. In addition, they are caused by blood vessels and hormone imbalance do. The vasodilatation is caused by various chemical mediators secreted by cells damaged by external stimuli. As the permeability increases, the antibody, complement, plasma, and phagocyte cells come to the inflammation site. This phenomenon causes erythema.

To eliminate inflammation, removal of inflammation source, biological reaction and symptoms that act to reduce the substance is called anti-inflammatory. To date, substances used for antiinflammatory purposes include nonsteroidal flufenamic acid, ibuprofen, benzydamine, indomethacin, and steroids such as prednisolone prednisolone, and dexamethasone. However, most of them have problems in terms of safety for skin or stability in cosmetics, and their use is limited.

In order to solve such problems, recently, a natural plant extract having excellent effects in terms of moisturizing, anti-aging, antioxidant, anti-inflammatory effect and improving skin condition or preventing skin aging or skin damage is contained as an active ingredient Many studies have been conducted on the cosmetic composition. However, in the case of a single application of a plant extract, there are many cases in which a slight skin improving effect is exhibited.

Therefore, there is a need to develop a natural material extract that can reduce skin irritation caused by chemical substances and improve skin safety and stability when containing cosmetics, while exhibiting an excellent skin improving effect.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems occurring in the prior art, and it is an object of the present invention to provide a cosmetic composition containing a natural material as an active ingredient to reduce skin irritation, And to provide a cosmetic composition for anti-aging and skin soothing that is excellent in skin soothing effect.

In order to achieve the above-mentioned object, one aspect of the present invention relates to a method for producing a plant extract, And a cosmetic composition comprising, as an active ingredient, a complex extract comprising a safflower extract, a peppermint extract, and a gossam extract.

In one embodiment, the content of each of the mongolian leaf myrtle extract and the complex extract may be 30 to 45% by weight and 25 to 50% by weight based on the total weight of the cosmetic composition.

In one embodiment, the combined extract may comprise 20 to 35 parts by weight of the extract, 45 to 60 parts by weight of the peppermint extract, and 25 to 40 parts by weight of the extract.

In one embodiment, the Leaf Mosquito Extract and the Combined Extract are selected from the group consisting of water, C ₁ -C ₄ lower alcohols, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, May be extracted with one solvent selected from the group consisting of

In one embodiment, the cosmetic composition may be for anti-aging or for soothing skin.

In one embodiment, the cosmetic composition is selected from the group consisting of softening agents, convergent lotions, nutritional lotions, lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, Or a combination of two or more thereof.

The cosmetic composition according to one aspect of the present invention includes a complex extract made of a mung bean leaf moth extract and a moss extract, a peppermint extract, and a gossam extract as an active ingredient, thereby reducing a user's skin irritation, The anti-aging effect can be maximized. In particular, the interaction between the extract of Mongolian leaf myrtle extract and the complex extract, and the interaction between the active ingredients in the complex extract, can enhance collagen synthesis, MMP-1 inhibition, and skin soothing effect.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

The cosmetic composition according to one aspect of the present invention is characterized in that it comprises: And a complex extract composed of a mushroom extract, a peppermint extract, and a ginseng extract as an active ingredient.

The " Sargassum & 　 muticum ( Yendo ) Fensholt "), also called dumpling bead mackerel, grows in the lower part of the intertidal zone of the Seongsan area in Jeju Island. The plant is greenish brown, with a short stem in the shape of a cylinder in the horn-shaped roots, and the stem is divided into several branches. Leaves are membranous or leathery and have a spatulate shape, with dull mounts at the edges but smooth. In addition, the bubbles are beads or eggs.

Mongolian leaf mackerel has been widely used in domestic and in Japan as S. Kjellmanianum It has been known as Yendo , but the specimens collected recently in Japan and in Japan have been reexamined and identified as S. muticum species.

Since the mongolian leaf mackerel is faded with seawater, the extract of the Mongolian mongolian leaf mackerel is rich in various nutrients, especially minerals, vitamins, and iron, thereby enhancing water resistance, enhancing elasticity, and protecting skin.

In addition, it contains a large amount of polyphenols and flavonoids, and the free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazide (DPPH) and the growth inhibition rate of the strain inoculation medium Activity has been reported to be excellent.

On the other hand, the complex extract may be composed of Sasa extract, Peppermint extract, and Gossam extract. The complex extract can exhibit excellent heat aging prevention and skin soothing effect by a synergistic effect depending on the interaction between the active ingredients derived from each raw material. Particularly, when the complex extract is applied to the skin together with the rich minerals, vitamins, and iron contained in the mongolian leaf myrtle extract, it can increase absorption rate and maximize collagen synthesis, MMP-1 inhibition, and skin soothing effect.

The complex extract may be extracted from the mixed pulverized product washed, dried and pulverized in a batch, and may be extracted from individual pulverized products each washed, dried and pulverized, and then mixed.

The " Pleuropterus " 　 multflorus ") is a vine-perennial plant of the dicotyledonous plant Pseudoptera spp., known to originate in China. It is a long-term vine medicinal plant with a height of 3-4 meters, It forms a root of root. It is widely known as a medicinal herb that has reddish-brown roots and is called "Sasuo". It strengthens the kidneys and strengthens the skin.

In addition, it improves the function of the liver to eliminate fatigue, it strengthens the skin, strengthens the bones and muscles, strengthens the heart, and is effective for nervous breakdown and insomnia. It is excellent for hematopoiesis, It is widely used for the treatment of impurities, uterine inflammation, chronic constipation.

The above-mentioned sewage contains starch, crude fat, emodin, chrysophanol, and lecithin as main ingredients, and is effective for tonic action, hypolipidemic depression and hypoglycemic action , And the lecithin is effective for antibacterial activity, antioxidant activity, anti-cancer activity, anti-inflammatory activity and the like.

Resveratrol, which is abundant in sewage, is a substance belonging to the polyphenol family, which is an antioxidant found in plants. Especially, resveratrol is contained in a large amount in red wine. Resveratrol has been known to have anticancer and antioxidant properties in the meantime, and it is also effective in preventing blood clotting, which can reduce heart arterial injury and cause heart attacks and strokes.

The "Peppermint (Mentha piperita ) "is a perennial herbaceous herbaceous perennial plant, which is distributed in Europe, Europe, America, Japan and China. It grows up to about 90㎝ in height, flowers are purple, bloom in June to July, and grow in low temperature and high humidity conditions.

The essential oil of the peppermint is contained in a large amount in the leaves. Menthol, which is a main component of the essential oil, provides a refreshing sensation to the skin and mucous membranes, assists in the care of the sensitive skin, and is effective in antibacterial and pain relief. In ancient Egypt, it was used as an edible, medicinal, and perfuming agent. In ancient Greece, it was the main ingredient of perfume. In Greece and Rome, it was used as an antioxidant and bath additive.

In addition, the menthol is effective for mental treatment, depression and nervous seizure, and it makes it cool when it is hot and warm when it is cold, which prevents mucus from leaking and also helps the fever and sweating. Accordingly, the essential oil is used for cold, asthma, bronchitis, cholera, pneumonia, pulmonary tuberculosis, food poisoning, neuralgia and the like.

Furthermore, the above-mentioned peppermint extract is used as a skin conditioning ingredient that functions as a refreshing sensation, a harmful foreign substance activity inhibition and elimination, and has a skin soothing effect. In addition, Effect.

The " Sophora flavescence "is a perennial herbaceous perennial plant with roots of dicotyledonous roots. It grows in sunny grasses of Korea, Japan, China, and Siberia. In June ~ August, a flower stalk of about 20㎝ comes out at the end of branches and butterfly-shaped light yellow flowers bloom, and flower length is 15 ~ 18㎜.

In one room, dried roots are called roasted roasted roasted roasted roasted roasted roasted roasted roasted ginseng. In the private sector, it is also used as an insecticide by dying stem or leaf.

In regard to the efficacy of the ginseng, the Korean traditional medicine book Donguibogam said, "The nature is not poisonous. It is a heat poisonous wind, which causes a burning of skin and flesh, and it treats the loss of eyebrows with red louse (lepidoptera). It heals the deep fever and sleeps, brightens the eyes and stops the tears. Look at the period of gait and it is better to have the yellow and red color of the dysentery and urine that are formed by latent heat. It cures toothache and acne. "

The roots of the ginseng root include matrine, oxymatrine, sophoranol, methylcytisine, anagyrine, baptifoline, sophocarpine, etc. Xanthohumol, isoxanthohumol, 3,4,5-trihydroxy-7-methoxy-8-isopentenylflavone (3,4,5-trihydroxy-7-methoxy- 8-isopentenylflavone), and 8-isopentenyl camphorol (8-isopentenyl camphorol). In addition, luteolin-7-glucoside is also contained in the stem and leaves.

The extract of Gosam exhibits a strong antibacterial activity against Propionibacterium acnes which is a causative agent of acne. Particularly, Matrine and oxymatrine in the components of the extract of Gosam extract have a central inhibitory action such as body temperature drop, It is known that it acts to block glutamic acid and to inhibit inflammation in specimens.

The "extract" refers to a solvent in which the active ingredient contained in the extraction raw material has been transferred by bringing the solvent and the extraction raw material into contact under specific conditions. If the substance is obtained by extracting the ingredients contained in the raw materials from natural materials, Regardless of the type. For example, extracting components dissolved in a solvent from natural products using water or an organic solvent, and extracting specific components of natural products, such as oils, and extracting only specific components.

On the other hand, the content of each of the mongolian leaf myrtle extract and the complex extract may be 30 to 45% by weight and 25 to 50% by weight based on the total weight of the cosmetic composition.

If the content of the mongolian leaf myrtle extract is less than 30% by weight, the effect of preventing heat aging and skin sedimentation derived from the active ingredient inherent to Mongolian leaf myrtle may not be properly realized. If the content is more than 45% by weight, May be relatively decreased and the synergistic effect due to the interaction of each component contained in the complex extract may not be properly realized.

If the content of the complex extract is less than 25% by weight, the interaction between the active ingredients contained in the complex extract may be insufficient, so that a synergistic effect may not be realized. If the content is more than 45% by weight, Not only the cost efficiency is lowered but also an excessive amount of extract is contained and the quality of the cosmetic composition product may not be realized.

The combined extract may comprise 20 to 35 parts by weight of the extract, 45 to 60 parts by weight of the peppermint extract, and 25 to 40 parts by weight of the extract.

When the content difference between the active ingredients constituting the complex extract is out of the above range, the synergistic effect due to the interaction between the active ingredients may not be properly realized. Therefore, The ratio can be appropriately controlled. The extract of Sasa extract, Peppermint extract, and Gossam extract may be mixed at a weight ratio of 1: 2: 1, and an optimal skin sedimentation and improvement effect can be realized within the above range.

On the other hand, the type of solvent in the extraction process is not particularly limited, and various solvents may be used as needed. In addition, the extraction can be carried out using a conventional method in the art such as cold-rolling, warming, heating, and the like using the solvent.

Accordingly, it is preferred that the mongolian leaf myrtle extract and the complex extract are selected from the group consisting of water, C ₁ -C ₄ lower alcohol, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, But it is not limited thereto.

Specifically, the extract of Momatobia miltiorrhizae can be extracted for 2 to 4 hours at a temperature of 15 to 30 ° C with ethanol as a solvent at a concentration of 60 to 80%.

That is, the extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent, and since the ethanol is excellent in selectivity for extraction of a physiologically active substance of natural raw materials, And a skin soothing effect can be realized. In addition, when ethanol is used as the extraction solvent, it is not only harmful to the human body but also suitable for use as an additive for foods, medicines and cosmetics, and increases in costs such as strict process control for securing safety There is no economic effect.

Particularly, the water and ethanol have different polarities, and the active ingredients to be extracted may be different depending on the polarity, so that the concentration of ethanol can be appropriately controlled so that optimal heat aging prevention and skin soothing effect can be realized. However, when the concentration of ethanol is less than 60%, the effective ingredient showing the skin improving effect may not be sufficiently extracted. If the concentration exceeds 80%, an appropriate yield may not be realized. Therefore, the concentration of ethanol may be appropriately adjusted within the range .

If the temperature is lower than 15 ° C, the extraction efficiency may be lowered and excessive time may be required, and the active ingredient may not be extracted sufficiently. If the temperature is higher than 30 ° C, some of the active ingredients may be deformed or lost, have. If the extraction time is less than 1 hour, the active ingredient may not be sufficiently extracted. If the extraction time is more than 4 hours, the extraction efficiency of the active ingredient may be lowered.

In addition, the complex extract can be extracted with water as a solvent at a temperature of 60 to 80 캜 for 10 to 16 hours. Since only water is used as a solvent, the active ingredient can be easily extracted, and a high concentration of active ingredient can be extracted with high extraction efficiency.

In addition, since it is not harmful to the human body, it can be suitable for use as an additive for foods, medicines and cosmetics, and it does not increase costs such as strict process control for securing safety, and therefore has an economical effect . If the temperature is lower than 60 ° C, the active ingredient may not be eluted sufficiently. If the temperature is higher than 80 ° C, the active ingredient contained in the complex extract may be deformed or disappear at a high temperature to prevent heat aging and skin soothing. If the extraction time is less than 10 hours, the active ingredient may not be sufficiently extracted. If the extraction time is longer than 16 hours, the extraction efficiency of the active ingredient may be lower than the time.

The cosmetic composition may be used for prevention of heat aging or skin regeneration and the cosmetic composition may be used as a softening agent, a convergent lotion, a nutritional lotion, a lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, , Spray, powder, and combinations of two or more thereof.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range not hindering the object of the present invention depending on the kind of the active ingredient, the purpose of use, and the like.

In addition, depending on the quality or function of the final product, it is possible to use various substances such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, Or auxiliaries commonly used in cosmetics or dermatology such as nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, and hydrophilic or lipophilic active agents . ≪ / RTI >

However, it is preferable that the adjuvant and the mixing ratio thereof are appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it should be apparent that the present invention is not limited by the following embodiments.

Manufacturing example  One : Mongolian leaf mushroom  Extract preparation

After washing the mongolian leaf mushroom, it was thoroughly dried at room temperature and pulverized to obtain 100 g of pulverized material.

100 g of the pulverized product was subjected to immersion extraction with a 10-fold volume of an aqueous ethanol solution (70% mass concentration) as a solvent at a temperature of 25 캜 for 3 hours. The extract was filtered with a filter and repeatedly extracted three times in the same manner with respect to the remaining raw materials to prepare an extract of Leaf moth. Then, the extract was concentrated under reduced pressure at 30 DEG C and lyophilized to obtain a solid content.

Manufacturing example  2: Preparation of complex extract

After washing with water, peppermint and gosam, they were thoroughly dried at room temperature and pulverized to obtain 100 g of pulverized products.

100 g of each pulverized product was subjected to immersion extraction with a 10-fold volume of water as a solvent at a temperature of 65 캜 for 12 hours. Each of the extracts was filtered with a filter, and the remaining extract was repeatedly extracted three times in the same manner and the obtained extracts were mixed to prepare a complex extract. Thereafter, the combined extract was concentrated under reduced pressure at 30 ° C and lyophilized to obtain a solid fraction.

The resultant complex extract was applied to a cosmetic composition, and the anti-aging effect and skin soothing effect of the cosmetic composition were verified.

Example  1: Compound extract and Mongolian leaf mushroom  Extract sample

32 parts by weight of the Mongolian leaf myrtle extract of Preparation Example 1 and 45 parts by weight of the combined extract of Sasa extract, Peppermint extract, and Gossam extract of Production Example 2 were mixed at a weight ratio of 1: 2: 1, respectively, .

Example  2: Compound extract and Mongolian leaf mushroom  Extract sample

32 parts by weight of the Mongolian leaf myrtle extract of Preparation Example 1 and 45 parts by weight of the combined extract of Sasa extract, Peppermint extract, and Gossam extract of Production Example 2 were mixed at a weight ratio of 1: 1: 1, respectively, .

Comparative Example  One : Mongolian leaf mushroom  Extract sample

A part of the mongolian leaf myrtle extract of Preparation Example 1 was isolated and used as a sample of Comparative Example 1.

Comparative Example  2: Compound extract sample

A part of the complex extract of Preparation Example 2 in which the extracts of Hashuo, Peppermint and Gosam were mixed at a weight ratio of 1: 2: 1 was separated to prepare a sample of Comparative Example 2.

Comparative Example  3: Compound extract sample

A part of the complex extract in which the Sasa extract, Peppermint extract, and Gosam extract of Preparation Example 2 were mixed at a weight ratio of 1: 1: 1 was separated to prepare a sample of Comparative Example 3.

Comparative Example  4 : Sewage  Extract sample

A part of the Sucrose extract of Preparation Example 2 was separated and used as a sample of Comparative Example 4.

Comparative Example  5: Peppermint extract sample

A part of the peppermint extract of Preparation Example 2 was isolated and used as a sample of Comparative Example 5.

Comparative Example  6: GoSam  Extract sample

A part of the crude extract of Preparation Example 2 was separated and used as a sample of Comparative Example 6.

Experimental Example  1: Evaluation of antioxidant effect

In order to evaluate the antioxidative effects of the samples according to Examples 1 and 2 and Comparative Examples 1 to 6, free radical scavenging activity was measured using DPPH purchased from Sigma Co., Ltd. (USA).

First, a standard DPPH solution having a concentration of 400 μM was prepared, and ethanol was added to ascorbic acid, which is an antioxidant, as a reference and a reference substance, respectively, to prepare a reagent having a concentration of 200 μg / ml. Next, the above reagents and a standard DPPH solution were added in the same proportions and stirred, followed by reaction at 37 ° C for 30 minutes, and the absorbance at 520 nm was measured. At this time, ethanol was added in place of the sample to give a control group.

The free radical scavenging ratio (%) was calculated using the following equation (1), and the results are shown in Table 1 below.

[Equation 1]

sample Free radical scavenging rate (%) Control group (no addition) 0 Ascorbic acid 42.73 Example 1 61.28 Example 2 57.33 Comparative Example 1 44.51 Comparative Example 2 48.86 Comparative Example 3 45.39 Comparative Example 4 43.48 Comparative Example 5 42.91 Comparative Example 6 43.55

As shown in Table 1, the samples (Examples 1 and 2) containing a mixture of the extract of Mongolian leaf myrtle extract and the compound extract (Example 2) contained a sample containing only ascorbic acid, a mongolian leaf myrtle extract or a complex extract (Comparative Examples 1 to 3) and the samples containing only one component of the complex extract (Comparative Examples 4 to 6), the free radical scavenging rate was remarkably increased.

In addition, the sample of Example 1 in which the Sucrose extract, the Peppermint extract, and the Gossam extract in the complex extract were mixed at a weight ratio of 1: 2: 1, respectively, was somewhat less than that of the sample of Example 2 mixed at a weight ratio of 1: And increased free radical scavenging rate.

These results suggest that not only the interaction between the mongolian leaf mushroom extract and the complex extract, but also the interaction between the mushroom extract, the peppermint extract and the ginseng extract in the complex extract have a synergistic effect on the increase of antioxidant activity.

Experimental Example  2: Evaluation of anti-inflammatory effect

In order to evaluate the anti-inflammatory effect of the samples according to Examples 1 to 2 and Comparative Examples 1 to 6, experiments were conducted to inhibit the production of nitrogen monoxide (NO) using Raw 264.7 cell line.

Raw 264.7 cells were stabilized at a concentration of 4 × 10 ⁴ cells / well and stimulated with 0.5 μg / ml Lipopolysaccharide (LPS) for 1 hour. L-NMMA (L-NG-Monomethylarginine), which is a positive control group, was treated with the above Examples 1 to 2 and Comparative Examples 1 to 6 and cultured for 24 hours. The nitric oxide production inhibiting ability was measured using a nitrogen monoxide quantitative kit and the following formula 2, and the results are shown in Table 2 below.

&Quot; (2) "

sample NO production inhibitory activity (%) The positive control (L-NMMA) 42.08 Example 1 44.19 Example 2 43.57 Comparative Example 1 35.64 Comparative Example 2 39.78 Comparative Example 3 36.19 Comparative Example 4 32.11 Comparative Example 5 29.94 Comparative Example 6 31.73

As shown in Table 2, the samples (Examples 1 and 2) containing a mixture of the extract of Mongolian leaf myrtle extract and the complex extract contained only L-NMMA, a mononitrate leaf meconium extract or a complex extract, It was confirmed that the nitrogen monoxide inhibitory ability was slightly increased as compared with the samples (Comparative Examples 1 to 3) and the samples containing only one component of the complex extract (Comparative Examples 4 to 6).

In addition, the sample of Example 1 in which the Sucrose extract, the Peppermint extract, and the Gossam extract in the complex extract were mixed at a weight ratio of 1: 2: 1, respectively, was somewhat less than that of the sample of Example 2 mixed at a weight ratio of 1: And showed an ability to inhibit nitrogen monoxide production.

Furthermore, it was confirmed that the inhibitory effect of nitrogen monoxide was decreased when only the extract of Montana leaf extract or the compound extract alone was used alone or when only one component of the compound extract was used, compared with the positive control (L-NMMA).

These results indicate that the synergistic effect of the mongolian leaf mung bean extract, the moss extract, the peppermint extract, and the complex extract containing the extract of Gossam ginseng is enhanced by the synergistic effect, and the inhibitory effect of nitrogen monoxide is improved. Accordingly, The composition is analyzed to exhibit an improved anti-inflammatory effect.

Experimental Example  3: Including sample Cosmetics  Evaluation of skin improvement effect of composition

The following experiment was conducted to evaluate the skin-improving effect of the cosmetic composition containing the samples obtained in Example 1 and Comparative Examples 1 to 2 and 4 to 6. [

First, each of the samples was formulated in the same cream as shown in Table 3 below, and samples of Example 1 and Comparative Examples 1 to 2 and 4 to 6 were different.

[Content (% by weight)] Raw material Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 4 Comparative Example 5 Comparative Example 6 Mongolian leaf mushroom extract
(Production Example 1) 32 77 - - - - Complex extract
(Production Example 2) 45 - 77 - - - Sashou extract
(Production Example 2) - - - 77 - - Peppermint extract
(Production Example 2) - - - - 77 - Gosam extract
(Production Example 2) - - - - - 77 Cetearyl alcohol 2 2 2 2 2 2 Cetearyl glucoside One One One One One One Jojoba seed oil 5 5 5 5 5 5 Silica 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 10 10 10 10 10 10 Purified water Balance Balance Balance Balance Balance Balance

For the 20 selected women aged 30 to 50 years, the cream of Comparative Examples 1 to 2 and 4 to 6 was applied to the right eye, and the cream of Example 1 was applied to the left eye twice a day for 3 months.

After 3 months, the degree of skin improvement was evaluated by subject's questionnaire and skin image analysis. The subjects' questionnaires were judged to be three stages of no improvement, slight improvement, and substantial improvement as compared with before use with regard to skin texture improvement and elasticity enhancement, and the results are shown in Table 4 below.

sample No improvement (persons) Some improvement (persons) Significant improvement (persons) Example 1 One 3 16 Comparative Example 1 11 5 4 Comparative Example 2 8 9 3 Comparative Example 4 12 8 0 Comparative Example 5 15 4 One Comparative Example 6 14 6 0

As shown in Table 4, compared with the case of using only the extract of Leaf Mosquito extract (Comparative Example 1), the case of using only the complex extract (Comparative Example 2) or the case of using only one component of the complex extract (Comparative Examples 4 to 6) It was confirmed that the skin texture improving effect and the elasticity improving effect were remarkably increased in the case of using all of the extracts of Mongolian leaf mushroom extract, Sucrose extract, Peppermint extract, and Gossam extract (Example 1).

Thus, the cosmetic composition including all of the complex extracts comprising the extract of Leaf moth, Leaf moth, Extract of Peppermint, and Gossam extract is superior to the cosmetic composition which is missing any effective ingredient. I can see that it is excellent.

Experimental Example 4: Evaluation of collagen synthesis amount

In order to measure the anti-aging effect of the samples according to Examples 1 to 2 and Comparative Examples 1 to 6, the effect of collagen synthesis was measured at 0.5% and 1.0% concentrations.

Human normal fibroblast cells were seeded at 1 × 10 ⁵ cells / well in a 24-well plate and cultured for 24 hours in 10% FBS / DMEM medium. Then, the cells were irradiated with infrared light and irradiated with serum free medium Each extract was treated and incubated in a CO ₂ incubator for 24 hours. Thereafter, the amount of collagen in the cell culture was converted to ng / ml by using a Procollagen Type C-peptide EIA kit (Takara, Japan), and the amount of collagen synthesized was measured. The results are shown in Table 5 below.

At this time, as a negative control for comparison of the anti-aging effect, samples were irradiated with infrared light and 0.3 μM ascorbic acid (vitamin C) was used as a positive control.

[Amount of collagen synthesis (ng / ml)] division 0.5% 1.0% Control group voice positivity Example 1 149 153 105 137 Example 2 136 141 Comparative Example 1 115 118 Comparative Example 2 127 132 Comparative Example 3 120 124 Comparative Example 4 112 115 Comparative Example 5 111 117 Comparative Example 6 114 121

As shown in Table 5, the samples containing the mixture of the Leaf mothballs extract and the complex extract (Example 1 and Example 2) showed an amount of collagen synthesis increased by 1.1 times as compared with that of the positive control, and the negative control The amount of collagen synthesis increased by 1.5 times compared with that of the control.

In addition, the amount of collagen synthesis was increased by 1.2 to 1.3 times as compared with the samples (Comparative Examples 1 to 6) in which only one component of the mixture was missing. As the concentration increased, the amount of collagen synthesis increased, It was confirmed that the prevention effect was improved.

Further, the sample of Example 1 in which the Sucrose extract, the Peppermint extract, and the Gossam extract in the combined extracts were mixed at a weight ratio of 1: 2: 1, respectively, was somewhat less than that of the sample of Example 2 mixed at a weight ratio of 1: Indicating increased amount of collagen synthesis.

These results indicate that the synergistic effect of the extract of Leaf moth, Leaf moth extract, Peppermint extract, and Gossam extract is enhanced by the interaction between the extract of Leaf moth, Leaf moth, Will exhibit an improved anti-aging effect.

Experimental Example 5: Evaluation of inhibitory effect of MMP-1

In order to measure the anti-aging effect of the samples according to Examples 1 to 2 and Comparative Examples 1 to 6, the inhibitory effect of collagen degrading MMP-1 at concentrations of 0.5% and 1.0% was measured.

Human normal fibroblast cells were inoculated on a 24-well plate at a density of 1 × 10 ⁵ cells / well and cultured for 24 hours. MMP-1 expression was increased by irradiation with infrared rays and MMP-1 inhibition was observed by treating each extract with concentration . The inhibitory effect of MMP-1 was evaluated by the following equation (3), and the results are shown in Table 6 below.

At this time, retinol (vitamin A) was used as a positive control for comparison of the anti-aging effect of the complex extract.

&Quot; (3) "

MMP-1 inhibitory activity (%) = (absorbance upon treatment with extract / absorbance upon non-treatment of extract) x 100

[MMP-1 inhibitory activity (%)] division 0.5% 1.0% Retinol (1 mg / ml) Example 1 42.97 63.21 42.03 Example 2 41.88 59.75 Comparative Example 1 21.44 33.51 Comparative Example 2 34.19 40.78 Comparative Example 3 31.21 37.28 Comparative Example 4 23.59 27.43 Comparative Example 5 25.81 36.22 Comparative Example 6 21.77 30.17

Referring to Table 6 above, the samples containing the mixture of Mongolian leaf myrtle extract and compound extract (Examples 1 and 2) showed equivalent or 1.5-fold increase inhibitory activity compared with the positive control.

In addition, it was confirmed that the inhibitory activity of MMP-1 was about twice as good as that of the samples lacking any one of the above-mentioned components (Comparative Examples 1 to 6), and 1.0% of the sample was treated with 0.5% The inhibitory activity was increased, and it was confirmed that the MMP-1 inhibitory activity was improved as the concentration increased.

Further, the sample of Example 1 in which the Sucrose extract, the Peppermint extract, and the Gossam extract in the combined extracts were mixed at a weight ratio of 1: 2: 1, respectively, was somewhat less than that of the sample of Example 2 mixed at a weight ratio of 1: Lt; RTI ID = 0.0 > MMP-1 < / RTI >

These results indicate that the synergistic effect of the mongolian leaf mushroom extract, the mushroom extract, the peppermint extract, and the ginseng extract-containing complex extract is enhanced by the interaction, and thus the MMP-1 inhibitory activity is enhanced. The inventive cosmetic composition is expected to exhibit an improved anti-aging effect.

Experimental Example  6: Evaluation of skin soothing effect (skin temperature reduction rate)

In order to evaluate the skin soothing effect of the cosmetic composition containing the samples obtained in Example 1 and Comparative Examples 1 to 2 and 4 to 6, the following experiment was conducted.

First, each of the samples was formulated in the same manner as shown in Table 7 below, and samples of Example 1 and Comparative Examples 1 to 2 and 4 to 6 were different.

[Content (% by weight)] Raw material Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 4 Comparative Example 5 Comparative Example 6 Mongolian leaf mushroom extract
(Production Example 1) 32 77 - - - - Complex extract
(Production Example 2) 45 - 77 - - - Sashou extract
(Production Example 2) - - - 77 - - Peppermint extract
(Production Example 2) - - - - 77 - Gosam extract
(Production Example 2) - - - - - 77 1,3-butylene glycol 3.0 3.0 3.0 3.0 3.0 3.0 glycerin 2.0 2.0 2.0 2.0 2.0 2.0 Oleyl alcohol 0.1 0.1 0.1 0.1 0.1 0.1 Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 ethanol 10.0 10.0 10.0 10.0 10.0 10.0 Benzophenol-9 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance

The subjects and their control samples were exposed to direct sunlight for 20 minutes before the test, and the test specimens (2 cm x 2 cm) were placed in the forearm. Using a Micro Pipette. The temperature was measured before application, immediately after application, 5 minutes after application, and 20 minutes after application. Water was used as a negative control for comparison of anti-aging efficacy. The temperature decrease rate of each sample after measurement was calculated according to the following Equation 4, and the results are shown in Table 8 below.

&Quot; (4) "

Temperature Reduction Rate (%) = (1-Temperature of Sample Application Site / Temperature of Non-use Site) × 100

[Temperature reduction rate (%)] division Immediately after application 5 minutes later After 20 minutes Control (water) 5.8 0.6 0 Example 1 13.8 5.3 2.1 Comparative Example 1 8.7 2.2 0.4 Comparative Example 2 10.1 2.6 0.9 Comparative Example 4 7.3 1.3 0.2 Comparative Example 5 6.9 1.4 0 Comparative Example 6 7.2 0.9 0.1

As shown in Table 8, the sample (Example 1) containing a mixture of the mung bean leaf moth extract and the complex extract showed a temperature reduction rate of at least 2 times higher than that of the negative control, (Comparative examples 1 to 2 and 4 to 6), and it was confirmed that the skin soothing effect was remarkably excellent.

In the case of the lotion containing the samples of Comparative Examples 1 to 2 and 4 to 6, the temperature decrease rate was 0 or less as time elapsed, The skin lotion (Example 1) showed a temperature reduction rate of 2.1% even after 20 minutes, indicating that it exhibited a constant skin soothing effect.

Thus, it can be seen that the cosmetic composition comprising all of the complex extracts comprising the mungbean leaf moth extract and the extract of Sasa extract, peppermint extract, and ginseng extract is superior to the cosmetic composition which is missing any effective ingredient have.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (6)

Mongolian leaf mushroom extract; And
Wherein the composition comprises 20 to 35 parts by weight of a water extract, 45 to 60 parts by weight of a peppermint extract, and 25 to 40 parts by weight of a ginseng extract as an active ingredient.
The method according to claim 1,
A cosmetic composition comprising 30 to 45% by weight of the mongolian leaf myrtle extract, and 25 to 50% by weight of the combined extract, based on the total weight of the cosmetic composition.
delete The method according to claim 1,
Wherein the mongol flower leaf extract and the complex extract are selected from the group consisting of water, a C ₁ to C ₄ lower alcohol, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, Of a cosmetic composition.
delete The method according to any one of claims 1, 2, and 4,
Wherein the cosmetic composition is at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, powder, Lt; RTI ID = 0.0 > 1, < / RTI >