KR102289532B1 - Livestock supplementary feed and a manufacturing method therefor - Google Patents
Livestock supplementary feed and a manufacturing method therefor Download PDFInfo
- Publication number
- KR102289532B1 KR102289532B1 KR1020210011949A KR20210011949A KR102289532B1 KR 102289532 B1 KR102289532 B1 KR 102289532B1 KR 1020210011949 A KR1020210011949 A KR 1020210011949A KR 20210011949 A KR20210011949 A KR 20210011949A KR 102289532 B1 KR102289532 B1 KR 102289532B1
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- South Korea
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- weight
- livestock
- lactobacillus
- culture solution
- feed
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Classifications
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- A—HUMAN NECESSITIES
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- Fodder In General (AREA)
Abstract
본 발명은 가축 보조사료의 조성물 및 이의 제조방법에 관한 것으로서, 구체적으로 가축 보조사료 조성물은 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물, 락토바실러스 파라카세이(Lactobacillus paracasei) 또는 상기 균주의 배양액의 건조물, 브로멜라인, 칼슘제제, 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모, 녹차 및 벌꿀 추출물, 및 포졸란을 포함하고, 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 이의 배양액의 건조물과, 락토바실러스 파라카세이(Lactobacillus paracasei) 또는 이의 배양액의 건조물의 중량비는 3:1인 것을 특징으로 한다.The present invention relates to a composition of an auxiliary feed for livestock and a method for producing the same, and more specifically, the composition for auxiliary feed for livestock is a dried product of Lactobacillus parafarraginis or a culture solution of the strain, Lactobacillus paracasei or the above. The dried product of the culture solution of the strain, including bromelain, calcium preparations, Saccharomyces crevisiae yeast, green tea and honey extracts, and pozzolan, and Lactobacillus parafarraginis (Lactobacillus parafarraginis) or its culture solution It is characterized in that the weight ratio of the dried product and the dried product of Lactobacillus paracasei or a culture solution thereof is 3:1.
Description
본 발명은 기능성 가축보조사료의 조성물 및 이의 제조방법에 관한 것으로서, 구체적으로 한우, 젖소와 같은 가축 소들의 건강 증진, 착유량 증가 등 생산성 증대, 축분의 악취 저감 등의 기능성을 기대할 수 있는 가축용 보조사료를 제공한다.The present invention relates to a composition of a functional livestock supplement and a method for manufacturing the same, and specifically, for livestock that can be expected to function, such as improving the health of livestock cattle such as Korean cattle and dairy cows, increasing productivity such as increased milking amount, and reducing odor of livestock manure Supplementary feed is provided.
축산업에 있어 가축질병은 영양학적 소실은 물론 경영상의 많은 문제를 가져오는 것으로 치료 보다는 예방이 중요하다고 인식되어 왔다. 현재까지 항생제는 질병예방과 치료 및 성장촉진을 위해 가축의 사료첨가제로서 널리 이용되어져 왔으며, 일반적으로 사용하는 항생물질로는 테트라사이클린계 (Tetracyline)와 페니실린계(Penicillin) 등이 있다(Boison 등, 1995). 그러나 항생물질에 대한 내성균의 출현과 축산물내에 항생물질의 잔류 등의 문제 때문에 가축의 질병치료 이외의 목적으로 사용하는 것은 금지되고 있다(Witte, 2000). 또한 생산된 축산물의 안전성에 대한 인식이 부각되면서 소비자는 안전하고 기능성이 강조되는 안전한 축산물을 선호하고 있는 실정이다. In the livestock industry, it has been recognized that prevention rather than treatment is more important than treatment as livestock diseases cause many management problems as well as nutritional loss. Until now, antibiotics have been widely used as feed additives for livestock for disease prevention, treatment, and growth promotion. Commonly used antibiotics include tetracyclines and penicillins (Boison et al., 1995). However, due to problems such as the emergence of antibiotic-resistant bacteria and the presence of antibiotics in livestock products, use for purposes other than the treatment of diseases in livestock is prohibited (Witte, 2000). In addition, as the awareness of the safety of the produced livestock products is highlighted, consumers prefer safe livestock products that emphasize safety and functionality.
따라서 천연자원으로부터 개발된 항생제 대체 물질에 대한 관심이 증가되고 이러한 천연자원의 우수한 기능은 가축의 질병을 예방할 뿐만 아니라 기능성 축산물을 생산하는데 매우 중요하다고 판단된다.Therefore, interest in antibiotic substitutes developed from natural resources is increasing, and the excellent function of these natural resources is judged to be very important not only to prevent diseases of livestock but also to produce functional livestock products.
현재 우리나라 축산업 중 가장 대표적인 축종은 한우이며, 한우의 전사육기간 중 송아지 (1일령~5개월령)기간에 급여되는 사료에만 항생제가 첨가되고 있다. 송아지 사육단계에서 항생제를 첨가하는 목적은 질병의 예방과 성장촉진을 목적으로 사용하고 있어 향후 친환경축산물 인증 및 무항생제 축산물의 생산에 있어 우선적으로 개선되어야 할 사항이다.Currently, the most representative livestock breed in Korea's livestock industry is Korean beef, and antibiotics are only added to the feed fed to the calves (1 day to 5 months of age) during the pre-breeding period of Korean cattle. The purpose of adding antibiotics in the calf breeding stage is to prevent diseases and promote growth, so it is a matter that needs to be improved first in the future certification of eco-friendly livestock products and the production of antibiotic-free livestock products.
한편 우유를 제공하는 젖소의 경우 최종 제품의 보건 안전성이 우선시되고 있기 때문에 질병을 예방과 치료효과가 높다고 하더라도 항생제를 사용하는 것은 조심스러울 수 밖에 없는 일이다.On the other hand, in the case of cows that provide milk, the health and safety of the final product is prioritized, so even if the prevention and treatment of diseases are high, the use of antibiotics has no choice but to be cautious.
최근에는 항생제 대체 물질로서 기능성 천연물질과 미생물을 이용한 비항생제적 방법들에 대해 연구가 진행되고 있다. 기능성 천연물질을 예를 들면, 녹차 (Green tea), 인진쑥 (Artemisia), 오가피 (Acanthopanax), 마늘(Garlic)과 감초 (Licorice) 등이 있다(Yang 등, 2003; Kwon 등, 2005). Recently, research on non-antibiotic methods using functional natural substances and microorganisms as an alternative to antibiotics is being conducted. Examples of functional natural substances include green tea, Artemisia, Acanthopanax, Garlic and Licorice (Yang et al., 2003; Kwon et al., 2005).
동물에 대한 천연물질의 급여 효과는 증체량(홍 등, 2002)과 사료효율(최 등, 1996; Gerbert 등, 1999)의 향상, 장내 환경의 개선(Ushid 등,2002), 혈청내 콜레스테롤의 수치를 낮추는(최 등, 1996; 홍 등, 2002) 등의 급여효과가 보고되었다. The effect of feeding natural substances on animals was improved in weight gain (Hong et al., 2002) and feed efficiency (Choi et al., 1996; Gerbert et al., 1999), improvement of the intestinal environment (Ushid et al., 2002), and serum cholesterol levels. A benefit effect such as lowering (Choi et al., 1996; Hong et al., 2002) has been reported.
또한 사료 첨가제로서 미생물제제(생균제)는 가축의 건강 유지와 생산성 향상 및 항생제를 대체하기 위하여 첨가하게 된다. 미생물제제(생균제)는 숙주의 장내 미생물 균총의 균형을 유지시켜 숙주 동물에게 이로운 영향을 미칠 수 있는 살아있는 미생물 사료 첨가제로서 유익한 미생물의 장내 우점을 유도하여 동물의 건강을 증진시키는 동물의 성장을 촉진시킬 수 있다고 알려져 있다(Fuller, 1989). In addition, microbiological agents (probiotics) as feed additives are added to maintain the health of livestock, improve productivity, and replace antibiotics. Microbial agents (probiotics) are live microbial feed additives that can have a beneficial effect on the host animal by maintaining the balance of the host's intestinal microbial flora. is known to be possible (Fuller, 1989).
미생물제제는 일반적으로 가축에게 급여되는 생균, 사균, 발효부산물들을 포함하며, 가장 대표적인 것들로는 락토바실러스, 바실러스, 효모, 소화효소제 및 이들의 복합제제가 있다. Scheuermann (1993)은 장내 효소의 활성을 증가시켜 사료 내 영양소의 이용률을 향상시킬 수 있으며, 면역 능력을 향상시켜 동물의 건강을 증진시킬 수 있다고 보고하였다(Fuller, 1989). Microbial preparations generally include live cells, dead cells, and fermentation by-products fed to livestock, and the most representative ones include lactobacillus, bacillus, yeast, digestive enzyme preparations, and combination preparations thereof. Scheuermann (1993) reported that the utilization of nutrients in feed can be improved by increasing the activity of intestinal enzymes, and the health of animals can be improved by improving immunity (Fuller, 1989).
한편, 다수 개체들이 공동으로 사육되고 있는 가축사 환경에서 사료의 공급 후 배설 축분의 악취가 계절, 날씨, 온도에 따라 증가되는 비위생적인 환경 문제가 유발되는 바, 사료의 공급시에도 건강, 영양상 기능 개선 뿐만 아니라 배설 이후의 축분에서 비롯되는 악취나 비위생적인 환경 개선도 고려되는 기능이 갈수록 요구되고 있는 실정이다.On the other hand, in an environment where a large number of individuals are bred jointly, an unsanitary environmental problem in which the odor of excreted livestock increases according to the season, weather, and temperature after supply of feed is caused. In addition to improving the function, there is a growing demand for a function that considers the improvement of the odor and unsanitary environment from livestock after excretion.
본 발명의 목적은 가축의 주 사료를 보조하는 기능적인 보조사료를 제공함에 있어, 높은 소화흡수율과 성장촉진을 통해 가축의 건강 및 영양개선 뿐만 아니라, 항균효과, 배설 후 축분으로 인한 악취 저감을 통한 환경개선의 다양한 기능성을 충족시킬 수 있는 보조사료와 이를 제조하는 방법을 제공함에 있다.An object of the present invention is to provide a functional supplementary feed that supplements the main feed of livestock, through high digestion and absorption rate and growth promotion to improve the health and nutrition of livestock, as well as to reduce odors due to antibacterial effect and livestock after excretion An object of the present invention is to provide a supplementary feed capable of satisfying various functions of environmental improvement and a method for manufacturing the same.
상기 목적을 달성하기 위한 본 발명의 일 실시예에 따른 가축 보조사료의 조성물은, 락토바실러스 파라파라지니스(Lactobacillus parafarraginis)의 생균제제 또는 생균 배양액의 건조물, 락토바실러스 파라카세이(Lactobacillus paracasei)의 생균제제 또는 생균 배양액의 건조물, 브로멜라인, 칼슘제제, 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모, 녹차 및 벌꿀 추출물, 및 포졸란을 포함한다.To achieve the above object, the composition of an auxiliary feed for livestock according to an embodiment of the present invention is a probiotic preparation of Lactobacillus parafarraginis or a dried product of a live cell culture medium, a probiotic preparation of Lactobacillus paracasei. or dried products of live cell cultures, bromelain, calcium preparations, Saccharomyces crevisiae yeast, green tea and honey extracts, and pozzolan.
이때 상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물과, 락토바실러스 파라카세이(Lactobacillus paracasei) 또는 상기 균주의 배양액의 건조물의 중량비는 3:1인 것을 특징으로 한다.At this time, the weight ratio of the dried product of the Lactobacillus parafarraginis or the culture solution of the strain and the dried product of the Lactobacillus paracasei or the culture solution of the strain is 3:1.
바람직하게는 상기 락토바실러스 파라파라지니스 및 락토바실러스 파라카세이의 1g당 생균수는 1.1x106 내지 1.1x108 일 수 있으나 이에 반드시 제한되는 것은 아니다.Preferably, the number of viable cells per 1 g of Lactobacillus paraparaginis and Lactobacillus paracasei may be 1.1x10 6 to 1.1x10 8 , but is not necessarily limited thereto.
본 발명에서 상기 효모의 중량비는 상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물의 중량비와 동일하거나 작을 수 있다.In the present invention, the weight ratio of the yeast may be the same as or smaller than the weight ratio of the dried product of the Lactobacillus parafarraginis or the culture solution of the strain.
또한 상기 브로멜라인은 상기 가축보조사료의 조성물의 총 중량대비 5중량%일 수 있다.In addition, the bromelain may be 5% by weight based on the total weight of the composition of the livestock supplement.
그리고 상기 칼슘제제는 상기 가축보조사료의 조성물의 총 중량대비 3.5중량%일 수 있다. 칼슘제제는 보조사료를 공급하는 대상 가축의 연령과 건강상태에 따라 조성함량을 조절할 수 있다.And the calcium preparation may be 3.5% by weight based on the total weight of the composition of the livestock supplement. The composition content of the calcium preparation can be adjusted according to the age and health status of the target livestock to which the supplementary feed is supplied.
여기에서 녹차 및 벌꿀 추출물은 고형추출물로 건조할 때 그 수분함량이 5 내지 15%로 건조된 분말일 수 있다.Here, the green tea and honey extract may be a dried powder having a moisture content of 5 to 15% when dried as a solid extract.
본 발명의 실시예에서 상기 포졸란은 상기 가축보조사료의 조성물의 총 중량대비 5중량%일 수 있다.In an embodiment of the present invention, the pozzolan may be 5% by weight based on the total weight of the composition of the livestock supplement.
본 발명의 실시예에 따른 가축보조사료의 조성물은 추가적으로 천연식물성 유산균, 천연젖산, 천연초산, 천연유기산을 더 포함할 수 있다.The composition of livestock supplement according to an embodiment of the present invention may further include natural vegetable lactic acid bacteria, natural lactic acid, natural acetic acid, and natural organic acid.
여기에서 추가되는 상기 유산균은 락토바실러스 하비엔시스(Lactobacillus harbinensis), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 바시노스테르쿠스(Lactobacillus vaccinostercus)일 수 있으나 이에 반드시 제한되는 것은 아니며, 유익균으로서의 유산균이면 추가될 수 있다.The lactic acid bacteria added here may be Lactobacillus harbinensis, Lactobacillus rhamnosus, or Lactobacillus vaccinostercus, but is not necessarily limited thereto, and as long as it is an additional beneficial bacteria, it may be can be
본 발명의 다른 실시예에 따른 보조사료의 제조방법은 혼합분말 원료의 제조단계, 주요 균주로서의 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 배양액을 제조하는 단계, 보조 균주로서의 락토바실러스 파라카세이(Lactobacillus paracasei) 배양액을 제조하는 단계, 상기 혼합분말 원료와 배양액을 혼합하는 단계, 이에 한번 더 발효종균을 접종하여 발효배합물을 제조하는 단계, 및 나머지 보조사료 배합물과 상기 발효배합물을 혼합하여 최종적으로 가축의 보조사료를 생산하는 단계로 구성된다.The method for producing an auxiliary feed according to another embodiment of the present invention includes the steps of preparing a mixed powder raw material, preparing a Lactobacillus parafarraginis culture solution as a main strain, and Lactobacillus paracasei as an auxiliary strain. Preparing a culture solution, mixing the mixed powder raw material and the culture solution, preparing a fermentation mixture by inoculating the fermented seed once more thereto, and mixing the remaining auxiliary feed mixture with the fermentation mixture to finally supplement the livestock feed It consists of steps to produce
구체적으로 상기 혼합분말 원료는 당밀 5~10중량%, 녹차 5~8중량%, 옥분 또는 보리맥강 5~10중량%, 소금 1.5~2중량%, 및 나머지는 쌀겨를 혼합하고 건조, 분쇄하여 제조한다.Specifically, the mixed powder raw material is prepared by mixing 5 to 10% by weight of molasses, 5 to 8% by weight of green tea, 5 to 10% by weight of jade powder or barley maekgang, 1.5 to 2% by weight of salt, and the remainder by mixing, drying and pulverizing rice bran. do.
주요 균주로서의 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 배양액은 유산균 배지에 락토바실러스 파라파라지니스(Lactobacillus parafarraginis)를 접종, 증식한 것이며, 보조 균주로서의 락토바실러스 파라카세이(Lactobacillus paracasei) 배양액은 유산균 배지에 락토바실러스 파라카세이(Lactobacillus paracasei)를 접종, 증식한 것이다.The Lactobacillus parafarraginis culture medium as the main strain is inoculated and propagated by Lactobacillus parafarraginis in the lactic acid bacteria medium, and the Lactobacillus paracasei culture medium as the auxiliary strain is the lactobacilli medium in the lactobacillus medium. Bacillus paracasei (Lactobacillus paracasei) was inoculated and propagated.
이때 상기 유산균 배지는 쌀겨 5~10중량%와, 당밀 15~20중량%와, 황설탕 4~8중량%, 및 나머지 물을 혼합한 혼합물일 수 있으나 이에 반드시 제한되는 것은 아니다. 경우에 따라서 벌꿀이나 올리고당과 같은 당분을 더 추가할 수 있다.At this time, the lactic acid bacteria medium may be a mixture of 5 to 10% by weight of rice bran, 15 to 20% by weight of molasses, 4 to 8% by weight of brown sugar, and the remaining water, but is not necessarily limited thereto. In some cases, additional sugars such as honey or oligosaccharides can be added.
생균 배양액을 제조할 때 이들 유산균 배지는 23~28℃로 유지하면서 2~3시간 주기로 공기를 공급하여 순환시켜 호기성 미생물의 생육조건에 부합하도록 처리된다.When preparing a live culture medium, these lactic acid bacteria medium are maintained at 23-28°C, and air is supplied and circulated every 2-3 hours to meet the growth conditions of aerobic microorganisms.
바람직하게는 25℃에서 2시간 간격으로 10m3/h의 공기를 주입하여 순환시키는 폭기과정을 거치면서 3~5일간 배양할 수 있다.Preferably, the culture can be carried out for 3 to 5 days while undergoing an aeration process in which air is injected and circulated at 25° C. at an interval of 2 hours at 10 m 3 /h.
이렇게 배양한 제1 생균 배양액인 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 배양액과, 제2 생균 배양액인 락토바실러스 파라카세이(Lactobacillus paracasei) 배양액을 2:1 또는 3:1의 중량비로 혼합한다. 이러한 중량비로 반드시 제한되는 것은 아니나, 제1 생균 배양액을 제2 생균 배양액보다 더 많은 중량비로 혼합한다.The first live cell culture medium, Lactobacillus parafarraginis, and the second live cell culture medium, Lactobacillus paracasei, are mixed in a weight ratio of 2:1 or 3:1. Although not necessarily limited to this weight ratio, the first live cell culture solution is mixed in a weight ratio greater than that of the second live cell culture solution.
혼합한 생균 배양액 60~70중량%과 상기 혼합분말 원료 30~40중량%을 혼합, 교반하여 배합물을 제조하고, 이러한 상기 배합물에 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 및 락토바실러스 파라카세이(Lactobacillus paracasei)를 포함하는 발효종균을 0.1~0.2중량%로 접종하여 4~10시간 동안 발효시킨 후 수분함량이 5~15%가 되도록 건조하여 발효배합물을 제조한다.60 to 70% by weight of the mixed live cell culture medium and 30 to 40% by weight of the mixed powder raw material are mixed and stirred to prepare a formulation, and Lactobacillus parafarraginis and Lactobacillus paracasei in this formulation ) is inoculated with 0.1 to 0.2% by weight of the fermentation starter, fermented for 4 to 10 hours, and then dried to obtain a moisture content of 5 to 15% to prepare a fermentation mixture.
마지막으로 브로멜라인 5중량%, 칼슘제제 3.5%, 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모 20중량%, 수분함량 5~15%로 건조된 녹차 및 벌꿀 추출물 10중량%, 및 포졸란 5중량%에 나머지 상기 발효배합물을 파쇄한 분말을 배합하여 가축 보조사료를 완성한다.Finally, bromelain 5% by weight, calcium preparation 3.5%, Saccharomyces crevisiae yeast 20% by weight, green tea and honey extract 10% by weight dried to 5-15% moisture content, and pozzolan 5 A supplementary feed for livestock is completed by mixing the powder obtained by crushing the rest of the fermentation mixture in wt%.
본 발명은 천연 미생물과 발효공법으로 제조한 가축의 보조사료를 제공함에 있어, 가축의 소화흡수를 개선하고 이로 인해 성장촉진과 육질을 개선할 수 있다.The present invention can improve the digestion and absorption of livestock, thereby promoting growth and improving meat quality, in providing a supplementary feed for livestock produced by natural microorganisms and a fermentation method.
한우의 경우 식체와 설사를 예방하고 잔병치레가 줄어들어 항생제 등을 과용하지 않아 건강이 증진되어 출하시기를 앞당길 수 있거나 번식우의 경우 수정이 잘되어 농가소득이 증대되는 효과를 기대할 수 있다.In the case of Korean beef, it is possible to prevent food intake and diarrhea, and to reduce the number of diseases caused by antibiotics, etc.
또한 젖소의 경우 천연 미생물 발효제제의 보조사료를 통해 식체와 설사를 방지하고, 유량 증가, 우유 품질 개선의 효과가 있으며, 체세포수가 조절되고 연변이 감소되는 효과가 있다.In addition, in the case of cows, the supplementary feed of natural microbial fermented preparations prevents eating and diarrhea, increases the flow rate, improves milk quality, regulates the number of somatic cells, and reduces soft stools.
이외에도 가축의 배설물에서의 악취가 저감되어 축사의 냄새가 개선되며, 위생적이고 쾌적한 축산환경을 도모할 수 있다.In addition, the odor from the excrement of livestock is reduced, the odor of the livestock is improved, and a sanitary and pleasant livestock environment can be promoted.
도 1은 본 발명의 일 실시예에 따른 가축 보조사료의 제조방법을 나타낸 흐름도이다.1 is a flowchart illustrating a method of manufacturing an auxiliary feed for livestock according to an embodiment of the present invention.
이하에서는 첨부한 도면을 참조하여 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시예를 상세히 설명한다. 그러나 본 발명은 다양한 변환을 가할 수 있고 여러가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다. 즉, 본 발명은 특정한 실시 형태에 한정되는 것이 아니며 본 발명의 사상 및 기술 범위에 포함되는 모든 변환, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily implement them with reference to the accompanying drawings. However, the present invention may apply various transformations and may be embodied in various different forms, and is not limited to the embodiments described herein. That is, it should be understood that the present invention is not limited to a specific embodiment, and includes all modifications, equivalents and substitutes included in the spirit and scope of the present invention.
본 발명의 일 실시예에 따른 가축 보조사료의 조성물은, 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물, 락토바실러스 파라카세이(Lactobacillus paracasei) 또는 상기 균주의 배양액의 건조물, 브로멜라인, 칼슘제제, 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모, 녹차 및 벌꿀 추출물, 및 포졸란을 포함한다.The composition of livestock supplemental feed according to an embodiment of the present invention is Lactobacillus parafarraginis or a dried product of a culture solution of the strain, Lactobacillus paracasei or a dried product of a culture solution of the strain, bromella phosphorus, calcium preparations, saccharomyces crevisiae yeast, green tea and honey extracts, and pozzolan.
이때 상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물과, 락토바실러스 파라카세이(Lactobacillus paracasei) 또는 상기 균주의 배양액의 건조물의 중량비는 2:1 또는 3:1인 것을 특징으로 한다.In this case, the weight ratio of the dried product of the culture solution of the Lactobacillus parafarraginis or the strain and the dried product of the culture solution of Lactobacillus paracasei or the strain is 2:1 or 3:1, characterized in that .
본 발명의 일 실시예에서 보조사료의 주된 미생물제제로 포함되는 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 락토바실러스 파라카세이(Lactobacillus paracasei)는 생균제제로 포함되거나 혹은 유산균 배지에 종균 접종하여 발효시켜 개체수를 증폭시킨 생균 배양액을 건조시킨 분말로 포함될 수 있다.In an embodiment of the present invention, Lactobacillus parafarraginis or Lactobacillus paracasei included as the main microbial agent of the supplementary feed is included as a probiotic agent or amplified by fermenting the lactic acid bacteria medium by seed inoculation. The prepared live cell culture solution may be included as a dried powder.
제1 생균으로서 락토바실러스 파라파라지니스를 제2 생균인 락토바실러스 파라카세이보다 더 많이 포함되도록 사용한다.As the first live bacteria, Lactobacillus paraparaginis is used so as to contain more than the second live cells, Lactobacillus paracasei.
락토바실러스 파라파라지니스는 동물의 장내에서 소화를 돕고 영양분의 흡수율을 증가시키며 장내 다른 유해 세균으로부터의 감염을 방어한다. 이러한 기능으로 인해 가축이 취약한 전염병으로부터 강건하게 질병을 예방할 수 있는 역할을 한다.Lactobacillus paraparaginis aids digestion in the intestines of animals, increases absorption of nutrients, and protects against infection from other harmful bacteria in the intestine. Due to these functions, livestock plays a role in robustly preventing diseases from susceptible infectious diseases.
또한 보조균주인 락토바실러스 파라카세이는 항암능력이 탁월하고 대장균의 활동을 억제하며 인산가수분해효소를 생성해 가축의 생육활성에 도움이 된다.In addition, the auxiliary strain, Lactobacillus paracasei, has excellent anticancer ability, inhibits the activity of E. coli, and produces phosphatase, which helps the growth of livestock.
상기한 락토바실러스 파라파라지니스나 락토바실러스 파라카세이 유산균은, 발효 사료 제조시 미생물(효모)이 호기성 발효를 일으키면서 진행되는 동안 발효사료 내부는 혐기적 조건이 되는 것으로, 이때 유산균은 혐기적 조건에서 병원성 미생물 및 부패균의 생육을 억제하는 역할을 한다.The above-mentioned Lactobacillus paraparaginis or Lactobacillus paracasei lactic acid bacteria is anaerobic conditions inside the fermented feed while microorganisms (yeast) cause aerobic fermentation during the production of fermented feed. It plays a role in inhibiting the growth of pathogenic microorganisms and putrefactive bacteria.
본 발명의 일 실시예에서는 대표적으로 두 가지 종류의 유산균을 포함하고 있으나 추가적으로 다른 유산균을 더 포함시켜 보조사료를 제조할 수 있다.In an embodiment of the present invention, two types of lactic acid bacteria are typically included, but supplemental feed can be prepared by additionally including other lactic acid bacteria.
추가될 수 있는 유산균으로서 락토바실러스 하비엔시스(Lactobacillus harbinensis), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 바시노스테르쿠스(Lactobacillus vaccinostercus)일 수 있으나 이에 반드시 제한되는 것은 아니다.Lactobacillus harbinensis (Lactobacillus harbinensis), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus vaccinostercus (Lactobacillus vaccinostercus) may be added as lactic acid bacteria that can be added, but is not necessarily limited thereto.
락토바실러스 하비엔시스는 균의 생장온도의 범위가 넓어 안정적으로 장에서 생존하기 때문에 동물의 장내 항균 작용을 지속적으로 안정화시킬 수 있으며, 락토바실러스 람노서스는 유해균이 내뿜는 독성물질을 감소시키고, 항체를 생산하는 면역계에서 중요한 역할을 하기 때문에 가축의 질병 관리와 예방에서 항생제의 기능을 대체할 수 있다. 또한 락토바실러스 바시노스테르쿠스는 전염성 바이러스 및 병원성 미생물을 예방하고, 암모니아와 황화수소 같은 악취를 저감하는 효과가 우수하다.Since Lactobacillus habiensis can stably survive in the intestine because it has a wide range of growth temperatures, Lactobacillus rhamnosus can reduce the toxic substances emitted by harmful bacteria and produce antibodies. Because it plays an important role in the immune system, it can replace the function of antibiotics in disease management and prevention in livestock. In addition, Lactobacillus basinostercus is excellent in preventing infectious viruses and pathogenic microorganisms and reducing odors such as ammonia and hydrogen sulfide.
본 발명의 가축 보조사료에 포함되는 생균의 균주 또는 배양액에서 생균수는 1g당 1.1x106 내지 1.1x108 일 수 있으나 이에 반드시 제한되는 것은 아니다.The number of viable cells in the strain or culture medium of live cells included in the livestock supplement of the present invention may be 1.1x10 6 to 1.1x10 8 per 1 g, but is not necessarily limited thereto.
본 발명의 보조사료에 포함되는 효모는 특별히 제한되지 않으나 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모와 같은 맥주 효모 계열 중에서 선택할 수 있다. The yeast included in the supplementary feed of the present invention is not particularly limited, but may be selected from brewer's yeast family such as Saccharomyces crevisiae yeast.
상기 효모제로 사용되는 미생물은, 물, 탄소원, 질소원, 무기물 및 비타민을 양분으로 하여 생육되는데, 탄소원은 주로 생활에 필요한 에너지를 만들고 질소원은 몸체를 구성하고 있는 단백질을 제조하게 되며 무기질과 비타민은 생육에 영향을 주게 된다. 이들 효모의 영양학적 특징은 단백질 함량이 45~48%로 매우 높고, 소화율이 80~90%이며, 아미노산 함량이 우수하고 비타민 B군 및 UGF를 상당히 많이 함유하고 있는 것이다. The microorganism used as the yeast agent is grown using water, a carbon source, a nitrogen source, minerals and vitamins as nutrients. will affect The nutritional characteristics of these yeasts are that the protein content is very high (45-48%), the digestibility is 80-90%, the amino acid content is excellent, and it contains quite a lot of vitamin B group and UGF.
따라서 본 발명의 보조사료에 사용되는 효모는 사전에 별도로 제조실에서 각각 원균배양한 후 혼합 할 수 있으며 그 중 사카로마이세스 크레비시에(saccharomyces crevisiae)효모는 기호성 및 소화율을 향상시키는 역할로 첨가될 수 있다.Therefore, the yeast used in the supplementary feed of the present invention can be separately cultured in a separate production room in advance and then mixed, and among them, Saccharomyces crevisiae yeast is added to improve palatability and digestibility. can be
본 발명의 실시예에서 보조사료에 포함되는 상기 효모의 중량비는 특별히 제한되는 것은 아니지만, 상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물의 중량비와 동일하거나 작을 수 있다. 이는 본 발명의 보조사료가 소화율과 건강 기능성을 향상시키며 가축의 다양한 기능성을 향상시킴에 있어 이들 효모제제가 락토바실러스 파라파라지니스와 상승적인 효과를 기대할 수 있기 때문이다.In an embodiment of the present invention, the weight ratio of the yeast included in the supplementary feed is not particularly limited, but may be the same as or smaller than the weight ratio of the dry matter of the Lactobacillus parafarraginis or the culture solution of the strain. This is because the supplementary feed of the present invention can be expected to have a synergistic effect with Lactobacillus paraparaginis in improving the digestibility and health functionality of the supplementary feed and improving various functions of livestock.
한편 본 발명에서 브로멜라인은 상기 가축보조사료의 조성물의 총 중량대비 1 내지 10중량%일 수 있으며, 바람직하게는 5중량%로 포함될 수 있다.Meanwhile, in the present invention, bromelain may be 1 to 10% by weight, preferably 5% by weight, based on the total weight of the composition of the livestock supplement.
브로멜라인은 파인애플의 줄기와 열매에 들어 있는 단백질 가수 분해 효소로서 시스테인 엔도펩티데이스로서 특이성이 넓고, 단백질 분자의 알라닌, 글리신, 라이신, 타이로신 펩타이드 결합의 가수 분해를 촉매하는 효소이므로 가축의 소화율을 촉진하는 기능을 할 수 있다.Bromelain is a proteolytic enzyme contained in the stem and fruit of pineapple. It has a wide specificity as cysteine endopeptidase. It is an enzyme that catalyzes the hydrolysis of alanine, glycine, lysine, and tyrosine peptide bonds in protein molecules. may have a stimulating function.
또한 본 발명에 포함되는 칼슘제제는 상기 가축보조사료의 조성물의 총 중량대비 3.5 내지 20중량%일 수 있으며, 바람직하게는 3.5 내지 10중량%일 수 있다. 칼슘제제는 보조사료를 공급하는 대상 가축의 연령과 건강상태에 따라 조성함량을 조절할 수 있는데, 연령이 어린 성장기 가축, 혹은 종계나 산란계의 경우 칼슘제제 함량을 더 높임으로써 골격 성장과 강화, 부족한 칼슘 보충을 위한 보조적인 기능을 부여할 수 있다.In addition, the calcium preparation included in the present invention may be 3.5 to 20% by weight, preferably 3.5 to 10% by weight, based on the total weight of the composition of the livestock supplement. The content of calcium preparations can be adjusted according to the age and health condition of the target livestock to be supplied with supplementary feed. An auxiliary function for supplementation can be given.
특별히 기능성으로 강화된 보조사료가 아닌 일반적 시판 사료의 경우 칼슘제제는 3.5중량%로 포함시킬 수 있다.In the case of a general commercially available feed that is not a supplemental feed specially fortified with functionality, the calcium preparation may be included in an amount of 3.5% by weight.
본 발명의 보조사료에 포함되는 녹차 및 벌꿀 추출물은 고형추출물로 건조할 때 그 수분함량이 5 내지 20%일 수 있으며 바람직하게는 10 내지 15%로 건조된 분말일 수 있다.The green tea and honey extracts included in the supplementary feed of the present invention may have a moisture content of 5 to 20% when dried as a solid extract, and preferably a dried powder to 10 to 15%.
녹차, 목초액, 칡 추출물은 주로 항산화 기능을 부여하여 가축의 기관 기능성을 향상시킬 수 있으며, 감초, 우엉, 치커리 추출물은 위점막 보호, 항균기능, 수분흡수, 비타민 공급 등의 효과가 있다. Green tea, wood vinegar, and arrowroot extracts mainly impart antioxidant functions to improve the organ function of livestock, while licorice, burdock, and chicory extracts have effects such as gastric mucosa protection, antibacterial function, water absorption, and vitamin supply.
또한 누에 추출물은 면역력 강화, 벌꿀 추출물은 비타민 등 각종 영양소 공급 및 면역력 향상에 효과가 있다. 이러한 동식물 추출물을 고형 건조물을 상황이나 환경에 따라 가변시켜 포함하여 보조사료의 기능성을 다변화하고 확장시킬 수 있다.In addition, silkworm extract is effective in strengthening immunity, and honey extract is effective in supplying various nutrients such as vitamins and improving immunity. It is possible to diversify and expand the functionality of the supplementary feed by including these animal and plant extracts by varying the solid dry matter according to the situation or environment.
본 발명의 실시예에서 상기 포졸란은 상기 가축보조사료의 조성물의 총 중량대비 5 내지 10중량%일 수 있으며 바람직하게는 5중량%일 수 있다.In an embodiment of the present invention, the pozzolan may be 5 to 10% by weight, preferably 5% by weight, based on the total weight of the composition of the livestock supplement.
포졸란은 항생제 내성균 및 장내 유해 미생물에 대한 항균활성이 확인됨에 따라 가축용 천연항균제 또는 보조사료로써 활용성이 있다. Pozzolan is useful as a natural antibacterial agent or supplementary feed for livestock as antibacterial activity against antibiotic-resistant bacteria and harmful intestinal microorganisms has been confirmed.
본 발명의 실시예에 따른 가축보조사료의 조성물은 추가적으로 천연식물성 유산균, 천연젖산, 천연초산, 천연유기산을 더 포함할 수 있다.The composition of livestock supplement according to an embodiment of the present invention may further include natural vegetable lactic acid bacteria, natural lactic acid, natural acetic acid, and natural organic acid.
여기에서 추가되는 상기 유산균은 유익균으로서의 유도산균이면 추가될 수 있다.The lactic acid bacteria added here may be added if the induced acid bacteria as beneficial bacteria.
본 발명의 다른 실시예에 따른 보조사료의 제조방법을 도 1의 흐름도를 참조하여 설명하기로 한다.A method of manufacturing an auxiliary feed according to another embodiment of the present invention will be described with reference to the flowchart of FIG. 1 .
도 1을 참조하면, 본 발명의 일 실시예에 따른 보조사료 제조방법은 혼합분말 원료의 제조단계(S1), 주요 균주로서의 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 배양액을 제조하는 단계(S2), 보조 균주로서의 락토바실러스 파라카세이(Lactobacillus paracasei) 배양액을 제조하는 단계(S3), 상기 혼합분말 원료와 각각의 생균 배양액을 혼합하여 배합물을 제조하는 단계(S4), 이에 한번 더 발효종균을 접종하고 발효배합물을 제조하는 단계(S5), 나머지 보조사료 배합물과 상기 발효배합물을 혼합하여 최종적으로 가축의 보조사료를 조성물을 배합하는 단계(S6), 및 보조사료를 완성하여 포장하고 출하하는 마지막 단계(S7)로 구성된다.Referring to Figure 1, the auxiliary feed manufacturing method according to an embodiment of the present invention comprises the steps of preparing a mixed powder raw material (S1), Lactobacillus parafarraginis as a main strain, preparing a culture solution (S2), Preparing a Lactobacillus paracasei culture solution as an auxiliary strain (S3), preparing a formulation by mixing the mixed powder raw material and each live cell culture solution (S4), Inoculating the fermented seed and fermenting it once more The step of preparing the formulation (S5), mixing the remaining auxiliary feed formulation with the fermentation mixture to finally mix the composition with the auxiliary feed for livestock (S6), and the final step of packaging and shipping the auxiliary feed (S7) ) is composed of
구체적으로 혼합분말 원료의 제조단계(S1)는 당밀 5~10중량%, 녹차 5~8중량%, 옥분 또는 보리맥강 5~10중량%, 소금 1.5~2중량%, 및 나머지는 쌀겨를 혼합하고 건조, 분쇄하여 제조한다.Specifically, the manufacturing step (S1) of the mixed powder raw material mixes 5-10 wt% of molasses, 5-8 wt% of green tea, 5-10 wt% of jade powder or barley maekgang, 1.5-2 wt% of salt, and the rest rice bran, It is prepared by drying and pulverizing.
가장 많은 함유량으로 포함되는 쌀겨(미강)와 보리맥강은 섬유질이 풍부하고, 발효시 유기산을 생성하기 때문에 항균성 및 항산화 효과가 뛰어나 별도의 항생제를 사용하지 않고도 이를 대체할 수 있는 천연 항생제 역할을 수행하므로 토지개량, 가축 사료 등에 매우 효과적이다.Rice bran (rice bran) and barley bran, which are contained in the highest content, are rich in fiber, and because they generate organic acids during fermentation, they have excellent antibacterial and antioxidant effects, so they act as natural antibiotics that can be replaced without the use of separate antibiotics. It is very effective for land improvement and livestock feed.
옥분은 토양 활성화에 따른 병충해를 예방하고, 가축사료로 첨가시에는 면역력을 증진시키는 기능을 위해 첨가된다.Jade powder is added to prevent diseases and pests caused by soil activation, and to enhance immunity when added to livestock feed.
당밀은 발효에 필요한 당분을 공급하고, 녹차는 항산화 기능을 부여하기 위해 추가될 수 있다. 소금은 천일염을 사용할 수 있으며, 미생물의 배양 및 발효 작용을 더욱 향상시키고 미네랄을 보충하는 역할을 한다.Molasses provides the sugars needed for fermentation, and green tea can be added to impart antioxidant properties. Sea salt can be used as salt, and it serves to further improve the culture and fermentation of microorganisms and supplement minerals.
상기 S1 단계와 별도로 순서에 관계없이 주요 균주로서의 락토바실러스 파라파라지니스 배양액과 보조 균주로서 락토바실러스 파라카세이 배양액이 제조된다(S2, S3).Separately from step S1, a Lactobacillus paraparaginis culture medium as a main strain and a Lactobacillus paracasei culture medium as an auxiliary strain are prepared regardless of the order (S2, S3).
쌀겨 5~10중량%와, 당밀 15~20중량%와, 황설탕 4~8중량%, 및 나머지 물을 혼합한 혼합물로 조제된 유산균 배지를 만들고, 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 발효종균을 접종하고 증식시켜 제1 생균 배양액을 제조한다.5 to 10% by weight of rice bran, 15 to 20% by weight of molasses, 4 to 8% by weight of brown sugar, and a mixture of the remaining water to prepare a lactic acid bacteria medium, Lactobacillus parafarraginis fermented spawn Inoculate and proliferate to prepare a first live cell culture solution.
상기 동일한 유산균 배지를 만들고 락토바실러스 파라카세이(Lactobacillus paracasei) 발효종균을 접종하고 증식시켜 제2 생균 배양액을 제조한다.To prepare the same lactic acid bacteria medium, Lactobacillus paracasei (Lactobacillus paracasei) fermented spawn is inoculated and proliferated to prepare a second live cell culture medium.
이때 유산균 배지에 발효 활성화를 위해 벌꿀이나 올리고당과 같은 당분을 더 추가할 수 있다.At this time, sugars such as honey or oligosaccharides may be further added to the lactic acid bacteria medium to activate fermentation.
당밀과 황설탕, 벌꿀이나 올리고당은 미생물 증식 배양의 먹이가 되는 물질이다.Molasses, brown sugar, honey, and oligosaccharides are substances that are food for microbial growth and culture.
생균 배양액을 제조할 때 이들 유산균 배지는 23~28℃로 유지하면서 2~3시간 주기로 공기를 공급하여 순환시켜 호기성 미생물의 생육조건에 부합하도록 처리된다.When preparing a live culture medium, these lactic acid bacteria medium are maintained at 23-28°C, and air is supplied and circulated every 2-3 hours to meet the growth conditions of aerobic microorganisms.
바람직하게는 25℃에서 2시간 간격으로 10m3/h의 공기를 주입하여 순환시키는 폭기과정을 거치면서 3~5일간 배양할 수 있으나 일반적으로 3일 배양으로 충분할 수 있다.Preferably, it can be cultured for 3 to 5 days while passing through an aeration process in which 10 m 3 /h of air is injected at 2 hour intervals at 25° C. and circulated, but in general, 3 days of culture may be sufficient.
이렇게 배양된 상기 제1 생균 배양액 및 제2 생균 배양액은 액상이며 각각 3:1의 중량비로 혼합한다. 본 발명의 기능성 보조사료의 기능성을 높이기 위해 락토바실러스 파라파라지니스 균주 또는 이의 배양액이 더 많은 함량이 되도록 포함시키는 것이다.The cultured first and second live cell cultures are liquid and are mixed in a weight ratio of 3:1, respectively. In order to increase the functionality of the functional supplementary feed of the present invention, the Lactobacillus paraparaginis strain or its culture solution is included in a larger content.
S4 단계에서 이들 미생물 배양액 60~70중량%과 상기 S1 단계에서 제조한 혼합분말 원료 30~40중량%을 혼합, 교반하여 배합물을 제조한다. In step S4, 60 to 70% by weight of the microbial culture medium and 30 to 40% by weight of the mixed powder raw material prepared in step S1 are mixed and stirred to prepare a formulation.
상기 S4 단계에서 제조된 배합물에 발효 종균을 0.1~0.2중량%로 접종하여 4~10시간 동안 발효시킨 후 수분함량이 5~15%가 되도록 건조하여 발효배합물을 제조한다(S5).The mixture prepared in step S4 is inoculated with a fermentation seed in an amount of 0.1 to 0.2% by weight, fermented for 4 to 10 hours, and then dried to a moisture content of 5 to 15% to prepare a fermentation mixture (S5).
이때 발효종균은 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 및 락토바실러스 파라카세이(Lactobacillus paracasei)일 수 있으나 이에 반드시 제한되는 것은 아니며, 바실러스 속 균주 또는 유산균을 포함할 수 있다. 바람직하기로는 페디오코커스 액시디락티시(Pediococcus acidilactici), 락토바실러스 플란타룸(Lactobacillus plantarum), 엔테로박터 호마에체이(Enterobacter hormaechei), 웨이셀라 헬레니카(Weissella hellenica), 크산토모나스 EBHRS01-2(Xanthomonas sp EBHRS01-2), 바실러스 서브틸리스(Bacillus subtilis), 바실러스 소노렌시스(Bacillus sonorensis), 바실러스터모아밀로보란스(Bacillusthermoamy lovorans), 류코노스톡 파라메센테로이드(Leuconostoc paramesenteroides), 페디오코커스 펜토사세이스 스트레인 LM2632(Pediococcus pentosaceis strain LM2632) 중에서 선택된 하나 또는 둘 이상이 혼합될 수 있다.At this time, the fermenting spawn may be Lactobacillus parafarraginis and Lactobacillus paracasei, but is not necessarily limited thereto, and may include Bacillus sp. strains or lactic acid bacteria. Preferably, Pediococcus acidilactici, Lactobacillus plantarum, Enterobacter hormaechei, Weissella hellenica, Xanthomonas EBHRS01-2 (Xanthomonas sp EBHRS01-2), Bacillus subtilis, Bacillus sonorensis, Bacillus thermoamy lovorans, Leuconostoc paramesenteroides, pe One or two or more selected from among Caucus pentosaceis strain LM2632 (Pediococcus pentosaceis strain LM2632) may be mixed.
4~10시간 동안 발효시키면서 저속으로 교반하여 균일한 발효와 발효물질이 혼합되도록 할 수 있다. 발효가 끝난 발효배합물은 통풍 건조하여 수분이 5~15%가 되도록 건조시킨다.While fermenting for 4 to 10 hours, it can be stirred at low speed so that uniform fermentation and fermented substances are mixed. After fermentation, the fermentation mixture is air-dried and dried so that the moisture content is 5~15%.
마지막으로 보조사료 조성물로서 브로멜라인 5중량%, 칼슘제제 3.5중량%, 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모 20중량%, 수분함량 5~15%로 건조된 녹차 및 벌꿀 추출물 10중량%, 및 포졸란 5중량%에 나머지 상기 발효배합물을 파쇄한 분말을 배합하여 가축 보조사료를 완성한다.Lastly, as a supplementary feed composition, 5 wt% of bromelain, 3.5 wt% of calcium preparation, 20 wt% of Saccharomyces crevisiae yeast, 10 wt% of green tea and honey extract dried to 5-15% moisture content %, and the powder obtained by crushing the remaining fermentation mixture in 5% by weight of pozzolan to complete the livestock supplement.
완성된 보조사료는 포장되어 출하하는데, 가축 농장에서 보조사료를 사용할 때에는 일반사료 1톤당 본 발명의 보조사료를 1~2kg으로 혼합하여 급여할 수 있다.The completed auxiliary feed is packaged and shipped. When auxiliary feed is used in livestock farms, 1 to 2 kg of auxiliary feed of the present invention per ton of general feed can be mixed and fed.
천연 미생물을 활용한 발효공법으로 제조한 본 발명의 보조사료를 급여할 경우 한우, 돼지, 육우, 염소 등의 가축은 병원 미생물과 가스가 억제되고 소화 흡수율이 증가되며 운동성이 증진된다. 배설 후 축분에서도 악취가 전폭적으로 저감되어 파리 등 곤충의 발생율이 저하되어 위생적인 축사 환경을 유지할 수 있다.When the supplementary feed of the present invention prepared by a fermentation method using natural microorganisms is fed, domestic cattle, pigs, beef cattle, goats, etc. are suppressed by pathogenic microorganisms and gases, digestion and absorption are increased, and motility is improved. Even in livestock manure after excretion, the odor is completely reduced, and the incidence of insects such as flies is reduced, so that a hygienic livestock environment can be maintained.
젖소의 경우 식체와 설사가 방지되고 체세포수가 조절되며 연변이 감소되고 운동성이 증진되어 우유의 품질이 향상되고 유량이 증가하는 효과가 있다.In the case of cows, feeding and diarrhea are prevented, the number of somatic cells is controlled, soft stools are reduced, and motility is enhanced, thereby improving milk quality and increasing milk flow.
양계의 경우 사료 효율이 증가되고 소화율이 높아져 육질이 개선되고 계분 악취가 감소한다. 또한 종계와 산란계의 경우 산란율이 증가하고 난황색이 좋아지며 난각이 두꺼워지는 등 계란의 품질이 향상되는 효과가 있다.In the case of poultry, feed efficiency is increased and digestibility is increased, which improves meat quality and reduces odor of chicken manure. In addition, in the case of breeders and laying hens, the egg quality is improved by increasing the laying rate, improving the yolk color, and thickening the eggshell.
하기는 본 발명이 일 실시예에 따른 보조사료 조성물의 함량비로 제조된 보조사료(시험구)와 일반 시중의 시판되는 보조사료(대조구)를 주 사료와 혼합하여 육우에게 공급하여 월령별로 체중과 증체량을 측정한 표를 개시하였다. The following is a supplementary feed (test group) prepared at the content ratio of the supplementary feed composition according to an embodiment of the present invention, and a commercially available supplementary feed (control group) in the general market, mixed with the main feed, and supplied to beef cattle, and the weight and weight gain by month A table in which the was measured was disclosed.
시험구 함량비:Test strip content ratio:
(파라파라지니스:파라카세이 =3:1 중량비 혼합)fermentation mixture
(Paraparaginis:Paracasei = 3:1 weight ratio mixing)
대조구(A사 보조사료제)Control group (company A supplemental feed)
실험결과표Experiment result table
*상기 1일당 증체량은 12개월 이후 15개월까지 성장한 육우의 체중을 60일로 나눔*The above-mentioned daily weight gain is divided by the weight of beef cattle grown from 12 months to 15 months by 60 days.
상기 표의 결과에서 알 수 있듯이, 본 발명의 보조사료를 혼합한 배합사료를 급여한 시험구 육우 개체들의 1일당 증체량이 일반시판의 보조사료를 혼합한 배합사료를 급여한 대조구의 육우개체들의 1일당 증체량에 비해 우수함이 입증되었다.As can be seen from the results of the above table, the daily weight gain per day of the beef cattle individuals in the test group fed the compound feed mixed with the supplementary feed of the present invention per day of the beef cows of the control group fed the formula feed mixed with the supplementary feed on the market It has been proven to be superior to the weight gain.
본 시험은 18개월까지 배식하여 측정하였는데, 지속적으로 본 발명의 보조사료를 혼합한 배합사료를 급여할 경우 육우들의 성장속도 증가하여 출하시기를 앞당기는 효과를 기대할 수 있을 것으로 보인다.This test was measured by feeding until 18 months, and if the compound feed mixed with the supplementary feed of the present invention is continuously fed, the growth rate of beef cattle increases and the effect of speeding up the shipping time is expected to be expected.
본 실험을 수행하면서 축사의 악취를 매일 아침 첫 급여 시간에 맞추어 측정한 결과 비록 관능적 검사 수준이기는 하나, 본 발명의 보조사료를 급여한 육우 축사에서의 축분 악취가 현저하게 확연히 감소하였음을 알 수 있었다.As a result of measuring the odor of the livestock barn at the time of the first feeding every morning while conducting this experiment, it was found that the odor of livestock manure in the cattle barn fed the supplementary feed of the present invention was significantly reduced, although it was at the sensory level. .
전술한 본 발명의 설명은 예시를 위한 것이며, 본 발명이 속하는 기술분야의 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 쉽게 변형이 가능하다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 예를 들어, 단일형으로 설명되어 있는 각 구성 요소는 분산되어 실시될 수 도 있으며, 마찬가지로 분산된 것으로 설명되어 있는 구성 요소들도 결합된 형태로 실시될 수 있다.본 발명에서 사용한 용어는 단지 특정한 실시예를 설명하기 위해 사용된 것으로, 본 발명을 한정하려는 의도가 아니다. 본 발명에서, "포함하다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허청구범위에 의하여 나타내어지며, 특허청구범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as being dispersed may be implemented in a combined form. It is used to illustrate the example, and is not intended to limit the present invention. In the present invention, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that it does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof. The scope of the present invention is indicated by the following claims rather than the above detailed description, All changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.
Claims (10)
상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis)의 생균제제 또는 생균 배양액의 건조물과, 락토바실러스 파라카세이(Lactobacillus paracasei)의 생균제제 또는 생균 배양액의 건조물의 중량비는 3:1이고,
상기 브로멜라인은 가축보조사료의 전체 조성물의 총 중량대비 5중량%이고, 상기 칼슘제제는 가축보조사료의 전체 조성물의 총 중량대비 3.5중량%인 것을 특징으로 하는 가축보조사료의 조성물.Lactobacillus parafarraginis (Lactobacillus parafarraginis) probiotics or dried products of live cell cultures, Lactobacillus paracasei (Lactobacillus paracasei) probiotics or dried products of live cultures, bromelain, calcium preparations, Saccharomyces crevice ( saccharomyces crevisiae) yeast, dried green tea and honey extract to 5-15% moisture content, and pozzolan,
The weight ratio of the dried product of the probiotic preparation or live culture solution of Lactobacillus parafarraginis and the dried product of the probiotic preparation or live cell culture solution of Lactobacillus paracasei is 3:1,
The bromelain is 5% by weight relative to the total weight of the total composition of the livestock supplemental feed, and the calcium formulation is 3.5% by weight relative to the total weight of the total composition of the livestock supplemental feed composition.
상기 락토바실러스 파라파라지니스 및 락토바실러스 파라카세이의 1g당 생균수는 1.1x106 내지 1.1x108 인 것을 특징으로 하는 가축보조사료의 조성물.According to claim 1,
The composition of livestock supplemental feed, characterized in that the number of viable cells per 1 g of the Lactobacillus paraparaginis and Lactobacillus paracasei is 1.1x10 6 to 1.1x10 8.
상기 효모의 중량비는 상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 또는 상기 균주의 배양액의 건조물의 중량비와 동일하거나 작은 것을 특징으로 하는 가축보조사료의 조성물.According to claim 1,
The weight ratio of the yeast is the same as or smaller than the weight ratio of the dry matter of the Lactobacillus parafarraginis or the culture solution of the strain.
상기 가축보조사료의 조성물은 천연식물성 유산균, 천연젖산, 천연초산, 천연유기산을 더 포함하는 것을 특징으로 하는 가축보조사료의 조성물.According to claim 1,
The composition of the livestock supplemental feed composition for livestock supplemental feed, characterized in that it further comprises natural plant lactic acid bacteria, natural lactic acid, natural acetic acid, and natural organic acid.
유산균 배지에 락토바실러스 파라파라지니스(Lactobacillus parafarraginis)를 접종, 증식한 제1 생균 배양액을 제조하는 단계,
유산균 배지에 락토바실러스 파라카세이(Lactobacillus paracasei)를 접종, 증식한 제2 생균 배양액을 제조하는 단계,
상기 혼합분말 원료 30~40중량%와 상기 제1 생균 배양액 및 제2 생균 배양액을 3:1의 중량비로 혼합한 혼합 생균 배양액 60~70중량%을 혼합, 교반하여 배합물을 제조하는 단계,
상기 배합물에 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 및 락토바실러스 파라카세이(Lactobacillus paracasei)를 포함하는 발효종균을 0.1~0.2중량%로 접종하여 4~10시간 동안 발효시킨 후 수분함량이 5~15%가 되도록 건조하여 발효배합물을 제조하는 단계, 및
브로멜라인 5중량%, 칼슘제제 3.5중량%, 사카로마이세스 크레비시에(saccharomyces crevisiae) 효모 20중량%, 수분함량 5~15%로 건조된 녹차 및 벌꿀 추출물 10중량%, 및 포졸란 5중량%에 나머지 상기 발효배합물을 파쇄한 분말을 배합하는 단계를 포함하는 가축보조사료의 제조방법.Molasses 5 to 10% by weight, green tea 5 to 8% by weight, jade powder or barley maekgang 5 to 10% by weight, salt 1.5 to 2% by weight, and the rest to prepare a mixed powder raw material by mixing, drying and pulverizing rice bran,
Inoculating Lactobacillus parafarraginis in the lactic acid bacteria medium, and preparing a first live culture medium grown,
Inoculating Lactobacillus paracasei into the lactic acid bacteria medium and preparing a second live cell culture medium,
Preparing a formulation by mixing and stirring 30 to 40 wt% of the mixed powder raw material and 60 to 70 wt% of a mixed live cell culture solution obtained by mixing the first live cell culture solution and the second live cell culture solution in a weight ratio of 3:1;
The mixture was inoculated with a fermented spawn containing Lactobacillus parafarraginis and Lactobacillus paracasei at 0.1 to 0.2% by weight and fermented for 4 to 10 hours, after which the moisture content was 5 to 15% drying to prepare a fermentation mixture, and
Bromelain 5% by weight, calcium preparation 3.5% by weight, Saccharomyces crevisiae yeast 20% by weight, green tea and honey extracts dried to 5-15% moisture content 10% by weight, and pozzolan 5% by weight A method of producing a supplementary feed for livestock comprising the step of blending the powder obtained by crushing the remaining fermentation mixture to %.
상기 유산균 배지는 쌀겨 5~10중량%와, 당밀 15~20중량%와, 황설탕 4~8중량%, 및 나머지 물을 혼합한 혼합물인 것을 특징으로 하는 가축보조사료의 제조방법.9. The method of claim 8,
The lactic acid bacteria medium is 5 to 10% by weight of rice bran, 15 to 20% by weight of molasses, 4 to 8% by weight of brown sugar, and a method for producing a supplementary feed for livestock, characterized in that a mixture of the remaining water.
상기 제1 생균 배양액을 제조하는 단계 및 제2 생균 배양액을 제조하는 단계는, 상기 유산균 배지를 23~28℃로 유지하면서 2~3시간 주기로 공기를 공급하여 순환시켜 상기 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) 및 락토바실러스 파라카세이(Lactobacillus paracasei)를 3~5일간 배양하는 것을 특징으로 하는 가축보조사료의 제조방법.9. The method of claim 8,
The step of preparing the first live cell culture solution and the step of preparing the second live cell culture solution include supplying and circulating air at intervals of 2 to 3 hours while maintaining the lactic acid bacteria medium at 23 to 28 ° C. parafarraginis) and Lactobacillus paracasei (Lactobacillus paracasei) manufacturing method of livestock supplement, characterized in that the culture for 3-5 days.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102474576B1 (en) * | 2022-03-03 | 2022-12-06 | 농업회사법인 주식회사 반디 | Eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria |
KR102836685B1 (en) * | 2024-06-17 | 2025-07-22 | 주식회사 밸류라움바이오 | Method for manufacturing specially mixed feed composition for functional beef production |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20080005037A (en) * | 2006-07-07 | 2008-01-10 | 순천대학교 산학협력단 | Functional Fermented Green Tea Microorganisms Using Green Tea and Useful Microorganisms, Method of Manufacturing the Same, Pork Breeding Method Using the Same and Pork Obtained therefrom |
WO2010047748A1 (en) * | 2008-10-20 | 2010-04-29 | Rema Balambika | Compositions of ground/powdered nuts/nut butters with curcuminoids /turmeric/mix having improved health benefits and oxidative stability |
KR20120022291A (en) * | 2010-09-01 | 2012-03-12 | 한국과학기술원 | A livestock feed additive comprising the fermented biotite using effective microorganisms for enhancing viability of livestock |
KR101175546B1 (en) * | 2009-05-01 | 2012-08-22 | 광주시농업기술센터 | Composite and process of manufacture that contain bromelain and rice bran fermented by available component |
CN104957399A (en) * | 2015-06-08 | 2015-10-07 | 宁波希诺亚海洋生物科技有限公司 | Pet deodorizing tablet and preparation method thereof |
KR20160007101A (en) * | 2014-07-11 | 2016-01-20 | 구본신 | Animal feed additive for new concept methane gas reduction |
KR20160116819A (en) * | 2015-03-31 | 2016-10-10 | 주식회사 모닝스타 | Composition of animal feed additive comprising pozzolan and use thereof |
US20160330998A1 (en) * | 2015-05-16 | 2016-11-17 | Big Heart Pet, Inc. | Palatable expanded food products and methods of manufacture thereof |
KR20190085568A (en) * | 2018-01-09 | 2019-07-19 | 인하대학교 산학협력단 | A high-protein animal feed using defatted microalgae and the manufacturing method thereof |
US20190262381A1 (en) * | 2012-02-22 | 2019-08-29 | Kemin Industries, Inc. | Animal feed compositions and methods of using the same |
KR20200095021A (en) * | 2019-01-31 | 2020-08-10 | 주식회사 슈퍼바이오 | Method for preparing calcium-fortified heat-resistant feed additive and feed additive prepared thereby |
KR102147602B1 (en) * | 2019-05-05 | 2020-08-24 | 조권석 | A process for the preparation of a feed composition for increasing immunity-boosting and the growth of pigs and the feed composition for increasing immunity-boosting and the growth of pigs prepared therefrom |
-
2021
- 2021-01-28 KR KR1020210011949A patent/KR102289532B1/en active Active
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20080005037A (en) * | 2006-07-07 | 2008-01-10 | 순천대학교 산학협력단 | Functional Fermented Green Tea Microorganisms Using Green Tea and Useful Microorganisms, Method of Manufacturing the Same, Pork Breeding Method Using the Same and Pork Obtained therefrom |
WO2010047748A1 (en) * | 2008-10-20 | 2010-04-29 | Rema Balambika | Compositions of ground/powdered nuts/nut butters with curcuminoids /turmeric/mix having improved health benefits and oxidative stability |
KR101175546B1 (en) * | 2009-05-01 | 2012-08-22 | 광주시농업기술센터 | Composite and process of manufacture that contain bromelain and rice bran fermented by available component |
KR20120022291A (en) * | 2010-09-01 | 2012-03-12 | 한국과학기술원 | A livestock feed additive comprising the fermented biotite using effective microorganisms for enhancing viability of livestock |
US20190262381A1 (en) * | 2012-02-22 | 2019-08-29 | Kemin Industries, Inc. | Animal feed compositions and methods of using the same |
KR20160007101A (en) * | 2014-07-11 | 2016-01-20 | 구본신 | Animal feed additive for new concept methane gas reduction |
KR20160116819A (en) * | 2015-03-31 | 2016-10-10 | 주식회사 모닝스타 | Composition of animal feed additive comprising pozzolan and use thereof |
US20160330998A1 (en) * | 2015-05-16 | 2016-11-17 | Big Heart Pet, Inc. | Palatable expanded food products and methods of manufacture thereof |
CN104957399A (en) * | 2015-06-08 | 2015-10-07 | 宁波希诺亚海洋生物科技有限公司 | Pet deodorizing tablet and preparation method thereof |
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