KR101175546B1 - Composite and process of manufacture that contain bromelain and rice bran fermented by available component - Google Patents

Abstract

The present invention discloses a cosmetic, pharmaceutical and health functional food or animal feed composition containing bromine and rice bran fermented product as an active ingredient, and a method for preparing the same, which are fermented by Bacillus subtilis as a first inoculum or a first method. Bacillus subtilis as an inoculum, and bromeliin and rice bran fermentation, which are naturally anaerobic fermented with lactic acid bacteria and yeasts, as an active ingredient, are included as active ingredients, which protect human skin, improve skin, skin whitening, moisturizing effect, exfoliation Not only improves atopic skin, but also as an antibacterial bactericidal effect, improves the quality of Korean beef, prevents or treats mastitis of cows, it is useful as a cosmetic composition, pharmaceutical composition, animal feed additives, and health functional foods. Will be.

Bromelain, rice bran fermentation product, moisture regulator, lactic acid bacteria, yeast

Description

Composition and preparation method for cosmetics or animal feed containing bromine and rice bran fermentation as active ingredients and methods for preparing the same {Composite and process of manufacture that contain bromelain and rice bran fermented by available component}

The present invention relates to a cosmetic or animal feed composition containing bromelain and rice bran fermentation as an active ingredient, and a technology for preparing the same, and more specifically, rice bran fermentation fermented with a bromeliin extracted from fruits or vegetables as a moisture control agent. Inoculate organic degrading microorganisms into water to allow natural anaerobic fermentation, or fermentation through artificial addition of lactic acid bacteria and yeast after inoculation of organic degrading microorganisms, containing bromine and rice bran fermentation as active ingredients. It relates to a cosmetic composition or a composition for livestock feed and a method for producing the same.

Since rice contains many nutritional ingredients, it has been used as a staple food for Koreans, and has been used for many purposes such as functional nutrition foods and beauty foods. Brown rice is rice that has been stripped of rice husk after drying and threshing rice. Brown rice is known as a high-nutrition food because it is composed of nutrients such as rice bran, rice snow, and endosperm, and recently, well-being trend It is becoming more in line with this.

Among them, rice bran, or rice bran, is rich in fats, proteins, essential fatty acids, essential amino acids, vitamins, dietary fiber and minerals. In particular, the vitamins contained therein include vitamin B, such as myoinositol, choline, and niacin, and vitamin E, an antioxidant that removes free radicals and free radicals that cause cellular aging and damage. Tocopherol) is also included.

In addition, the mineral contains phosphorus, potassium, magnesium, calcium, etc., a recently reported protein that acts to lower blood cholesterol, cytosterol and blood circulation, skin metabolism, whitening and sunscreen effect It is also known to contain components such as gamma-oryzanol.

Despite the excellent nutritional and potent value of rice bran, it is regarded as a by-product of rice milling, and its use is limited to simple rice bran oil, feed, etc., without a systematic study of its efficacy.

As already known, rice bran fermented product contains abundant amino acid group which is a main ingredient of natural moisturizing factor, and has an effect of imparting shine and moisture to skin. In addition, a large amount of remaining organic acids, sugars and peptides keep the skin moist and give a refreshing feeling.

Bromelain, on the other hand, is a proteolytic enzyme that is extracted from vegetables or fruits (for example, pineapple, kiwi, papaya, banana, sesame, etc.) to promote digestion and is used as a flavoring agent in food additives. It is also used for anti-inflammatory, obstetric treatment and to enhance the effects of other drugs. It has also been reported to be effective in preventing E. coli diarrhea in piglets (TL Mynott et al., Gut. 38:28). 32, 1996; DS Chandler & TL Mynott, Gut. 43: 196-202, 1998).

In addition, bromelain has been reported to degrade serum fibrin, inhibit the synthesis of fibrinogen and inhibit malignant cell growth, and the application of bromelain to wounds is known to inhibit or prevent edema. (SJ Taussing & S. Batkin, J. ethno-pharmacol. 22: 191-203, 1988; Lotz-Winter, H., Planta medica 56: 249-253, 1990).

Therefore, the composition containing the rice bran fermentation as an active ingredient together with the bromelain is expected to be effective in improving the beauty of skin or meat of livestock, the treatment of inflammatory diseases, and thus the need for development thereof has emerged.

The present invention is inoculated with organic degrading microorganisms in fermented rice bran fermented with bromelain extracted from vegetables or fruits as a moisture regulator cosmetics and pharmaceuticals containing health and anaerobic fermentation bromine and rice bran fermentation as active ingredients Antioxidant for protecting human skin, improving skin, whitening skin, moisturizing effect, exfoliating skin, improving atopy skin, as well as harmful bactericidal effect, improving meat quality of Korean cattle, and mastitis of cow The purpose of the present invention is to be useful as a cosmetic composition, a pharmaceutical composition, additives such as livestock feed, and health functional food.

In addition, the present invention is effective in bromine and fermented rice bran fermented through artificial addition of lactic acid bacteria and yeast after inoculating organic degrading microorganisms into fermented rice bran fermented with bromelain extracted from vegetables or fruits as a moisture control agent It is another object to provide a composition for cosmetics or animal feed containing as a component.

The composition for cosmetics or animal feed containing bromine and rice bran fermentation as an active ingredient of the present invention for achieving the above object, containing bromine and containing rice bran fermentation, more specifically pineapple containing bromline It contains fermented rice bran fermented as an active ingredient as a moisture control agent.

In addition, the cosmetic or animal feed composition is fermented from the inoculation of organic degradation microorganisms.

In addition, the organic degradation microorganism is a Bacillus subtilis (bacillus subtilis).

In addition, the cosmetic or animal feed composition is a fermentation is made from the artificial addition of lactic acid bacteria and yeast.

In addition, the lactic acid bacteria is a high temperature lactic acid bacteria, the yeast is to include Saccharomyces cereviaiae (saccharomyces cereviaiae).

On the other hand, as a method for producing a cosmetic or animal feed composition containing bromelain and rice bran fermentation as an active ingredient, (a) pineapple containing bromline and rice bran are mixed to form a rice bran fermentation containing a certain amount of water Making; And (b) inoculating the composition of step (a) and fermenting the organic degraded microorganism as a first inoculum; It includes.

In addition, (c) inoculating and incubating the organic degradation microorganisms as a first inoculum in step (b), and then adding the lactic acid bacteria and yeast as a second inoculum to the culture to anaerobic fermentation; .

In addition, the organic degrading microorganism is used Bacillus subtilis (Bacillus subtilis).

In addition, in the step (a), the content of pineapple containing bromelain in the total content of the composition is 10 to 50% by weight, the content of rice bran is mixed by 50 to 90% by weight of water content 15 to 50% by weight It is made to be.

In addition, in the step (c), the lactic acid bacteria and yeast is inoculated with 0.1 to 1% of 100 parts by weight of the medium.

In addition, the lactic acid bacteria include the use of thermophilic lactic acid bacteria, and the yeast, including the use of saccharomyces cereviaiae (saccharomyces cereviaiae).

Such cosmetic and pharmaceutical and health functional food or animal feed compositions of the present invention are fermented by Bacillus subtilis as the first inoculum, or by natural anaerobic fermentation with lactic acid bacteria and yeast as the first inoculum and Bacillus subtilis It contains bromine and rice bran fermentation as active ingredients, which protects human skin, improves skin, whitens skin, moisturizes, exfoliates, improves atopic skin, as well as sterilizing harmful bacteria, improves meat quality of cows, and cows. Antioxidant function for the prevention or treatment of mastitis can be expected to be useful as a cosmetic composition, pharmaceutical composition, additives such as livestock feed, and health functional foods.

Hereinafter, an embodiment of the present invention will be described.

The composition for cosmetics or livestock feed containing bremline and rice bran fermentation according to the present invention as an active ingredient comprises the steps of (a), (b), (c).

Step (a);

A pineapple containing bromelain and rice bran are mixed to form a rice bran fermentation product containing a certain amount of water. First, the pineapple containing bromelain is roughly ground and mixed with rice bran.

At this time, the rice bran and pineapple is the content of pineapple is 10 to 50% by mass, and the content of the rice bran is mixed by 50 to 90% by mass with respect to 100% by mass of the medium to form a medium with a moisture content of 15 to 50%.

That is, the pineapple contains bromelain (bromelain), the bromelain is extracted from the pineapple to promote digestion with proteolytic enzymes and is used as a flavoring agent in food additives. It is also used to treat anti-inflammatory, obstetric treatment, and enhance the effects of other drugs. It has also been reported to be effective in preventing E. coli diarrhea in piglets (TL Mynott et al., Gut. 38: 28 ~). 32, 1996; DS Chandler & TL Mynott, Gut. 43: 196-202, 1998).

In addition, bromelain has been reported to degrade serum fibrin, inhibit the synthesis of fibrinogen and inhibit malignant cell growth, and the application of bromelain to wounds is known to inhibit or prevent edema. (SJ Taussing & S. Batkin, J. ethno-pharmacol. 22: 191-203, 1988; Lotz-Winter, H., Planta medica 56: 249-253, 1990)

Step (b);

Inoculating and fermenting the organic degradation microorganism as a first inoculum to the composition of step (a), 0.1 to 1 by liquid culture of the Bacillus subtilis, an organic degradation microorganism as a first inoculum in the medium composition of step (a) After inoculating%, the mixture was incubated for 24 hours by aeration with agitation at an initial temperature of 30 ° C and a fermentation temperature of 30 to 40 ° C.

Here, the Bacillus subtilis does not cause disease or toxicity in humans, animals, plants, etc., and does not have potential risks, and thus releases strong amylase and various proteases to decompose various natural substances while decomposing various natural substances. It helps to circulate, so it is preferable to use those which grew in the temperature of 37-40 degreeC, and pH in the range of 6.8-7.0.

Step (c);

After inoculating and incubating the organic degrading microorganism as the first inoculum in step (b), by adding lactic acid bacteria and yeast as a second inoculum to the culture and fermentation, the culture of the step (b) and lactic acid bacteria again Yeast is added to the natural anaerobic fermentation.

At this time, lactic acid bacteria and yeasts are inoculated by 0.1 to 1%, respectively, based on the weight of the medium, and the time required for natural anaerobic fermentation is 3 to 5 days based on the outdoor temperature of 25 degrees, or 32 to 3 days or 15 depending on the outside temperature. The fermentation time of 7 days is required in the figure, and the most suitable temperature is 32 degrees.

The lactic acid bacteria are widely distributed in the natural world up to the digestive tract or dozens of agricultural products of humans or animals, and in particular, they are attached to the intestinal epithelial cells and decompose lactic acid, fatty acids, antibiotics, hydrogen peroxide, and the like to inhibit harmful bacteria. It is known that not only enhances immunity but also enhances the nutritional value of foods, and bacteria belonging to these lactic acid bacteria include dozens of species, such as Streptococcus, Tediococcus, Leukonostock, Lactobacillus and Bifidus, and used in the present invention. The type of lactic acid bacteria that can be used is not particularly limited, but thermophilic lactic acid bacteria are suitable, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus plantarum, Lactobacillus delbruekii, Lactobacillus Lactobacillus johnsonii, Lactobacillus rhamnosus, Lactobacillus casei, Streptococcus thermophilus, Streptococcus pydobacterium pydobacterium pydomium pythium (Bifidobacterium bifidum), Bifidobacterium infantis, Bifidobacterium longum, Pediococcus pentosaceus, Leuconosstock mesenteroides, etc. Can be used, in the present invention was used Lactobacillus casei (Lactobacillus casei).

In addition, the yeast is not particularly limited, Saccharomyces cerevisiae (Saccharomyces cerevisiae), Saccharomyces sakei, Xanthophyllomyces dendrorhous (Xanthophyllomyces dendrorhous) In the present invention, Saccharomyces cerevisiae was used.

Therefore, a cosmetic or animal feed composition containing bromine and rice bran fermented product as an active ingredient is produced by the above-described manufacturing method, and the cosmetic or animal feed composition prepared as described above may protect human skin, improve skin, skin whitening, In addition to exfoliation and atopic dermatitis, it is useful as an additive for cosmetic compositions, pharmaceutical compositions, and health functional foods as it has an antioxidant function for enhancing immunity of animals, reducing inflammation, preventing or treating cow mastitis.

In this case, when usefully used in the health functional food, bromine and rice bran fermented product as active ingredients, including one or more excipients and additives generally used in the manufacture of the composition and health functional food composition of the art It can be easily prepared in various forms according to known methods.

Here, the health functional food includes all forms such as functional food, nutritional supplement, health food and food additives.

For example, as a health food, the composition itself containing the bromelain and rice bran fermentation product of the present invention as an active ingredient is prepared in the form of tea (rice bran tea), juice and drink of [Example 3] to be described later to drink. Or granulated, encapsulated and powdered.

In addition, in order to use the composition containing the bromelain and rice bran fermentation products of the present invention as an active ingredient in the form of a food additive, it may be prepared in powder or concentrate form.

As a preferable content of the composition containing the bromelain and rice bran fermentation product of this invention as an active ingredient in the health functional food of this invention, about 0.1-10 mg or more per 100g of foods may be changed, and such content is changeable.

On the other hand, in the case of the pharmaceutical composition alone or a composition containing bromine and rice bran fermentation product prepared according to the method of the present invention as an active ingredient or one or more pharmaceutically acceptable carrier, excipient or diluent further You may.

Pharmaceutically acceptable in the above refers to a composition which is physiologically acceptable and, when administered to humans, typically does not cause an allergic or similar reaction.

Examples of such carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oils.

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The pharmaceutical composition may further include fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers and preservatives.

In addition, the pharmaceutical composition containing bromine and the rice bran fermentation product of the present invention as an active ingredient may be administered through various routes including oral, transdermal, subcutaneous, intravenous or intramuscular, and the effective dosage of the pharmaceutical composition may be It may be appropriately changed depending on age, weight, health condition, sex, route of administration and duration of treatment.

In the case of the cosmetic composition includes bromine and rice bran fermented product prepared according to the method of the present invention as an active ingredient, in addition to the components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, Conventional adjuvants and carriers such as vitamins, pigments and flavorings.

However, the cosmetic composition according to the present invention may be prepared in any formulation commonly prepared in the art, but is not limited thereto, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders. And soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like of Formulation Example 2 described below.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

In addition, the cosmetic composition according to the present invention may be blended with the cosmetic composition containing bromine and rice bran fermented product of the present invention as an active ingredient with respect to the total cosmetic composition in an amount of about 20 mass% to 100 mass% of the total cosmetic composition. Can be.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

[ Example  One]

After sterilizing 10 kg of rice bran (white powder: white portion cut out of rice) by autoclaving, ozone washing 4 kg of pineapple, roughly crushing the ozone washed pineapple and mixing with the rice bran, the water content is 33% The medium was prepared. Then, Bacillus subtilis was inoculated with 1% by liquid culture, and then incubated for 24 hours by aeration with agitation at an initial temperature of 30 ° C and a fermentation temperature of 30-40 ° C.

Then, lactobacillus casei, a high temperature lactic acid bacterium, and Saccharomyces cerevisiae, a yeast bacterium, were added, followed by natural anaerobic fermentation.

At this time, lactic acid bacteria and yeast are inoculated 0.5% by weight of each medium, and the time required for natural anaerobic fermentation is fermented for 5 days at 25 ° C outside air temperature, and then air-dried until the final moisture content reaches 12%. To prepare a cosmetic and pharmaceutical and health functional food or animal feed composition containing the fermented rice bran as an active ingredient.

[ Example  2]

100 kg of rice bran (powdery: white portion cut out about 11% of rice) and 20 kg of pineapple were roughly ground and mixed with rice bran, and the medium content was 20%. Then, Bacillus subtilis was inoculated with 1% by liquid culture, and then incubated for 24 hours by aeration with agitation at an initial temperature of 30 ° C and a fermentation temperature of 30-40 ° C.

Then, lactobacillus casei, a high temperature lactic acid bacterium, and Saccharomyces cerevisiae, a yeast bacterium, were added, followed by natural anaerobic fermentation.

At this time, lactic acid bacteria and yeasts are inoculated 1% by weight of each medium, and the time required for natural anaerobic fermentation is fermented for 5 days at 25 ° C outside air temperature, so that the final moisture content of bromine and rice bran fermentation is effective. Cosmetics, pharmaceuticals and health functional foods or livestock feed compositions were prepared.

[ Example  3]

100 kg of rice bran (pulverized phase: white portion cut out about 11% of rice) and 65 kg of pineapple were roughly ground and mixed with rice bran to form a medium having a water content of 49%. Then, Bacillus subtilis was inoculated with 1% by liquid culture, and then incubated for 24 hours by aeration with agitation at an initial temperature of 30 ° C and a fermentation temperature of 30-40 ° C.

Then, lactobacillus casei, a high temperature lactic acid bacterium, and Saccharomyces cerevisiae, a yeast bacterium, were added, followed by natural anaerobic fermentation.

At this time, lactic acid bacteria and yeasts are inoculated 1% by weight of each medium, and the time required for natural anaerobic fermentation is fermented for 5 days at 25 ° C outside air temperature to make up cosmetics and pharmaceuticals containing bromelain and rice bran fermentation as active ingredients. And a health functional food or a composition for livestock feed was prepared.

[ Example  4]

Example 4 is the same as Example 2 except for using Lactobacillus ashophilus instead of Lactobacillus casei in Example 2 containing bromelain and rice bran fermentation as an active ingredient Cosmetic and pharmaceutical and health functional food or animal feed composition was prepared.

[ Example  5]

Example 5 is the same as Example 2 except for using Lactobacillus bulgaricus instead of Lactobacillus casei (Lactobacillus casei) in Example 2 cosmetics containing bromelain and rice bran fermentation as an active ingredient And pharmaceutical and health functional food or animal feed compositions.

[ Example  6]

Example 6 in the same manner as in Example 2 except for using Lactobacillus rhamnosus instead of Lactobacillus casei (Lactobacillus casei) in Example 2 cosmetic containing bromine and rice bran fermentation as an active ingredient And pharmaceutical and health functional food or animal feed compositions.

[ Example  7]

Example 7 was used in the same manner as in Example 2 except for using Saccharomyces sakei instead of Saccharomyces cerevisiae in Example 2, bromine and rice bran fermentation. Cosmetics, pharmaceuticals and health functional food or animal feed composition containing the active ingredient was prepared.

[ Experimental Example  One]

Furthermore, experiments were conducted to determine whether the cosmetics, pharmaceuticals, and health functional foods or livestock feed compositions containing brimline and rice bran fermented product prepared in Example 2 of the present invention had an antimicrobial effect. The pathogens were Staphyloccus aureus and Escherichia coli, which showed a significantly higher infection rate in people with atopic dermatitis. The bacteria were multiplied by the shaking flask method.

Figure 1 relates to a general rice bran inoculated with 1% by mass of general rice bran, 0.1 mass% of liquid cultured Staphylococcus aureus (7.0 × 10 ⁵ cfu / ml) to maintain 37 ℃ after shaking incubation for 24 hours The result of diluting the culture by ⁴ times with dilution in Staphylococcus aureus differentiation medium (3M) was grown at 37 ° C. for 24 hours. As a result, the number of viable cells increased after 24 hours. Able to know.

Figure 2 relates to the cosmetic composition and pharmaceutical and health functional food or animal feed composition prepared in Example 2 inoculated 1 mass% of composition, 0.1 mass% of liquid cultured Staphylococcus aureus (7.0 × 10 ⁵ cfu / ml) in distilled water After the shaking was incubated for 24 hours at 37 ° C., the culture was diluted 10 ⁴ times with dilution in Staphylococcus aureus differentiation medium (3M) and grown at 37 ° C. for 24 hours. After 24 hours, 98-100% were killed and the antimicrobial activity was excellent.

Figure 3 relates to the general rice bran inoculated with 1% by mass of general rice bran, 0.1 mass% of liquid cultured E. coli (2.0 × 10 ⁵ cfu / ml) to maintain 37 ℃ incubation for 24 hours after the incubation of E. coli discrimination Diluting the culture by dilution in the medium (3M) 10 ⁴ times, the result was grown at 37 ℃ for 24 hours, as a result the number of viable bacteria increased after 24 hours, it can be seen that there is no antimicrobial activity.

Figure 4 relates to the cosmetic composition and pharmaceutical and health functional food or animal feed composition prepared in Example 2 inoculated with 1 mass% of composition, 0.1 mass% of liquid cultured E. coli (2.0 × 10 ⁵ cfu / ml) in distilled water 37 Shaking incubation was carried out for 24 hours while maintaining the ℃, the culture was diluted 10 ⁴ times by dilution in E. coli differentiation medium (3M) and grown at 37 ℃ for 24 hours, as a result, E. coli 98 ~ 100 after 24 hours % Is killed and the antibacterial activity is excellent.

From the results of FIGS. 1 to 4, it is understood that the cosmetic and pharmaceutical and health functional food or animal feed composition containing bromine and rice bran fermented product of the present invention as an active ingredient has an antimicrobial effect that enhances immune function. Therefore, the present invention can provide a composition for immuno-enhancing through the composition and pharmaceutical and health functional food or animal feed composition containing bromine and rice bran fermented product prepared by the methods of the present invention as an active ingredient. It is.

[ Experimental Example  2]

As human skin ages, its function decreases due to various internal and external factors, and in particular, the amount of collagen that occupies most of the dermis, which is part of the skin, decreases, and the activity of fibroblasts that synthesize collagen also decreases. As the synthesis decreases, skin aging progresses, the thickness of the epidermis becomes thinner as a whole, dehydration of cells and tissues occurs, and the wrinkles increase and the wrinkles gradually deepen.

Therefore, in order to check whether the cosmetic and pharmaceutical and health functional food or animal feed composition containing bromine and rice bran fermented product of the present invention can be utilized as functional additives, the composition prepared in [Example 2] 10g of nonwoven fabric was immersed in 37 ℃ wash and bath water for 5 minutes, and then used twice a week for 4 weeks to find out whether it was satisfactory and the number of panels was 30.

very good satisfied usually dissatisfaction Moisturizing 24 6 0 0 Skin whitening 9 15 6 0 Exfoliation 21 6 3 0 Skin trouble prevention 13 16 One 0 Cleaning power 9 17 4 0

As shown in Table 1, the satisfaction level was very high in all investigation fields such as moisturizing power, skin whitening, exfoliation, skin trouble prevention, and cleansing power.

As such, no panel was found to be unsatisfactory when the composition of the present invention containing bromelain and rice bran fermented product as an active ingredient was used for washing and bathing. Six people (20%) came out.

As described above, the cosmetic and pharmaceutical and health functional food or animal feed compositions containing bromelain and rice bran fermentation according to the present invention as active ingredients are highly functional, such as moisturizing, skin whitening, exfoliation, skin trouble prevention, and cleaning power. It was found that excellent, according to the bath agent of [Example 1] below, and the soap of [Example 2] can be prepared and utilized.

[ Formulation example  One]

Bathing agent: A bathing agent is prepared by putting 100% by mass of the composition containing the bromelain and the rice bran fermentation product of Example 1 as active ingredients in a nonwoven fabric.

[ Formulation example  2]

Baru: 20 mass% of the composition containing bromine and rice bran fermented product of Example 2 as active ingredients, and 80 mass% of soap base (synthetic combination such as palm nug, glycerin, and surfactant) based on 100 mass% of the whole soap. Will be mixed.

That is, with respect to 100% by mass of the total soap, the soap base is dissolved in a hot bath while maintaining the temperature of 75 ℃ ~ 85 ℃ to form a transparent cosmetic soap base of 80% by mass, then the completely dissolved transparent soap base 80 20% by mass of the composition containing bromelain and rice bran fermentation as an active ingredient is added to the mass%, completely mixed, and the soap is manufactured by filling the mold.

[ Formulation example  3]

Rice bran tea: Rice bran tea was prepared by putting 100% by mass of a composition containing bromine and rice bran fermentation product as an active ingredient in a nonwoven fabric.

[ Formulation example  4]

Hanwoo high-grade meat feed: 12% by mass of the composition containing bromine and rice bran fermentation of Example 2 as active ingredients, 20% by weight of corn flakes, 5% by weight of concentrated feed, 10% by weight of protein skin, based on 100% by weight of feed , 6% by weight of soybean hull, 3% by weight of barley, 2% by weight of lupine, 2% by weight of cottonseed, 5% by weight of ink, 11% by weight of bean curd, 8% by weight of mushroom medium, 5% by weight of molasses, 8% by weight of straw Vitamin, salt, sodium bicarbonate, limestone, etc.) by mixing 3% by mass, to prepare a high-quality meat feed of late Han beef.

Therefore, when feeding the above feed to the cattle, bromine included in the feed has a function to promote digestion, fat, protein, essential fatty acids, essential amino acids, vitamins, dietary fiber and minerals contained in the rice bran fermentation While the nutritional and efficacy of the function is being exercised, it can help improve the quality of the beef.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. It is to be understood that such changes and modifications are within the scope of the claims.

1 is a photograph of inoculation of the culture by Staphylococcus aureus inoculated liquid culture in the general rice bran by dilution in Staphylococcus aureus differentiation medium (3M) for 24 hours at 37 ℃.

Figure 2 is inoculated with a liquid cultured Staphylococcus aureus inoculated into the composition of Example 2 of the present invention, the culture was diluted by dilution in Staphylococcus aureus differentiation medium (3M) and grown at 37 ° C. for 24 hours.

Figure 3 is inoculated with E. coli liquid cultured to the general rice bran and cultured by dilution in E. coli differentiation medium (3M) and grown at 37 ℃ for 24 hours.

Figure 4 is inoculated with E. coli cultured liquid culture in the composition of Example 2 of the present invention after diluting the culture by the dilution method in the E. coli differentiation medium (3M) photographed at 37 ℃ for 24 hours.

Claims (15)

Pineapple containing bromine, which is a moisture control agent, and rice bran fermentation product prepared by mixing rice bran with the pineapple containing bromelain; Bacillus subtilis, which is an organic degradation material that is first inoculated to ferment the rice bran fermentation product; And, high temperature lactic acid bacteria and Saccharomyces yeast bacteria inoculated secondly to naturally anaerobic fermentation of the rice bran fermentation inoculated the Bacillus subtilis, the organic degradation material first; Cosmetic or animal feed composition containing bromine and rice bran fermentation comprising as an active ingredient. delete delete delete According to claim 1, The lactobacillus genus pyrogenic lactic acid bacteria, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus casei, A cosmetic or animal feed composition containing bromine and rice bran fermentation as an active ingredient, characterized in that any one of the selective use. According to claim 1, wherein the Saccharomyces spp. Yeast, For cosmetics or animal feed containing bromine and rice bran fermentation products as active ingredients, characterized in that either Saccharomyces cerevisiae or Saccharomyces sakei is optionally used. Composition. (a) mixing the pineapple containing bromelain and rice bran to form a rice bran fermentation containing a certain amount of water; And (b) fermenting the composition of step (a) by first inoculating Bacillus subtilis, which is an organic degradation microorganism, as a first inoculum; And (c) inoculating Bacillus subtilis, which is an organic degradation microorganism as a first inoculum in step (b), and then incubating the thermophilic lactic acid bacteria of Lactobacillus and yeast of Saccharomyces as a second inoculum to the culture. Second inoculation to natural anaerobic fermentation; Method for producing a cosmetic or animal feed composition containing bromine and rice bran fermentation as an active ingredient, characterized in that it proceeds. delete delete According to claim 7, wherein the composition of the step (a), the content of pineapple containing bromelain in the total content of the composition is 10 to 50% by weight, the content of rice bran is composed by mixing 50 to 90% by weight A method for producing a cosmetic or animal feed composition comprising bromine and rice bran fermentation as an active ingredient. The method of claim 10, wherein the water content of the composition of step (a) is 15 to 50% by weight, characterized in that the bromine and rice bran fermented product containing a cosmetic or animal feed composition for producing a composition for animal feed. The method according to claim 7, wherein in step (c), the lactobacillus pyrogenic lactic acid bacteria and Saccharomyces yeast bacteria is inoculated at 0.1 to 1% relative to 100 parts by weight of the medium bromine and rice bran fermentation Method of producing a cosmetic or animal feed composition containing as an active ingredient. The method according to claim 7, wherein the lactobacillus genus thermophilic lactic acid bacteria, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus casei, Method for producing a cosmetic or animal feed composition comprising bromine and rice bran fermentation product as an active ingredient, characterized in that any one of the selective use. The method of claim 7, wherein the Saccharomyces spp. For cosmetics or animal feed containing bromine and rice bran fermentation products as active ingredients, characterized in that either Saccharomyces cerevisiae or Saccharomyces sakei is optionally used. Method of Preparation of the Composition. delete

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