KR101966188B1 - Cosmetic Compositions for Anti-oxidation and Anti-wrinkling Comprising Extracts of Plants - Google Patents

Abstract

The present invention relates to a method for the production of a plant of the genus Saccharomyces, such as Suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill.) K. Koch. , Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima and Fatsia japonica (Thunb.) Decne. & Planch. The present invention relates to a cosmetic composition for antioxidation and wrinkle improvement, which comprises as an active ingredient an extract of at least one selected from the group consisting of Mizushima and Fatsia japonica (Thunb.) Decne .
Since the extract provided from the present invention is excellent in DPPH scavenging activity and collagen production efficacy, it can be used as a cosmetic composition for antioxidation and wrinkle improvement.

Description

[0001] Cosmetic Compositions for Anti-oxidation and Anti-wrinkling Comprising Extracts [

The present invention relates to a method for the production of a plant of the genus Saccharomyces, such as Suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill.) K. Koch. , Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima and Fatsia japonica (Thunb.) Decne. & Planch. The present invention relates to a cosmetic composition for antioxidation and wrinkle improvement, which comprises as an active ingredient an extract of at least one selected from the group consisting of Mizushima and Fatsia japonica (Thunb.) Decne .

Active oxygen introduced from the outside of the living body or generated in the living body promotes aging of the living body or causes cancer. Therefore, studies on antioxidants that inhibit oxidation by active oxygen are under way, and phenolic compounds, flavonoids, tocopherol, vitamin C, selenium and the like are known. However, the natural antioxidants do not have a practically sufficient effect when applied to the skin. On the other hand, antioxidant activity is excellent and low-priced synthetic antioxidants have a problem that their use is limited due to safety concerns such as human side effects.

In addition, collagen is a major substrate protein produced in fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, division of cells and differentiation Induction of growth of an organism or wound healing) are known. Such collagen is reduced by aging and photo aging caused by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin. In recent years, extensive research into skin aging has been developed and the important functions of collagen in the skin have been revealed. Effective ingredients promoting collagen synthesis and exhibiting wrinkle-reducing effects are known. For example, retinoic acid, transforming growth factor (TGF), animal placenta-derived protein, betulinic acid, and chlorella extract are known as collagen synthesis promoting substances. However, the above-mentioned effective ingredients are limited in the use amount due to safety problems such as irritation and redness when applied to the skin, or have insufficient effect, so that the effect of improving the skin function by promoting the collagen synthesis of the skin can not be expected.

On the other hand, according to recent natural-oriented and environment-friendly consumption tendency, a natural substance derived from a plant or an animal is mixed with various forms based on its functionality and used in cosmetics. In recent years, there has been a demand for so- And the interest in healthy eating and health and prevention has been attracting attention.

Suaeda malacosperma H. Hara is a perennial herbaceous herbaceous perennial plant of the dicotyledonous plant. It is a name for grass that grows in the area where sea water and fresh water meet. Leaves are slanting, linear, fleshy and pointed. The bottom is dome, the top is flat, and the vertical line is like a trail in the center. The length is 1 to 5 cm and the width is 0.2 to 0.6 cm. It is often white with a white or green color, with large leaves and small leaves. Flowers bloom in yellowish green in the leaf axil above the stem in August ~ September, forming the water inflorescence. There is no peduncle. There are five pieces of horns and egg-shaped. There are 5 stamens, a little longer than the husk, and the anther is yellow. There are two styles. The fruit is bag-shaped sac and 囊 果, which is similar to the fruit of the secondary fruit, but it is slightly smaller in size and rounded at the end. It is similar to Sehonghwamun, but it does not bloom much, and like the cushion, the upper side of the leaf is flat, but it is much wider and more sparse. The fruit is like a star like a star, but its size is slightly smaller and the end is round. It is similar to the turfgrass, but the branches are different from the lower 2 ~ 3㎝ above the stem. In other words, it looks like a combination of sea horsetail, cedar herb, bilingual herb, and turmeric. It is a species reported only in 2001 because it is difficult to distinguish it from similar species.

As a composition technique using an extract of Suaeda malacosperma H. Hara , Korean Patent Registration No. 10-1715622 entitled " Antimicrobial Composition Containing Extract of Botanicals from the Southeast of Japan, as an Active Ingredient " There is still no technology for a cosmetic composition capable of exhibiting antioxidant and wrinkle-improving effects.

Sedum oryzifolium Makino is a perennial herbaceous perennial plant, which is a perennial herbaceous perennial plant of the dicotyledonous plant Rosaceae. It grows on the rocky side of the beach. It is about 10cm in height and it is gathered. The stem extends sideways with many branches, and the upper part and branches of the main stem gather and stand straight. Leaves are shifted, conical eggs are inverted or elliptical, with blunt ends and no petiole. Flowers bloom in June to July in yellow, and flower ears are often divided into three. It has five petals, wide and narrow, sharp and pointed. Calyx is five ovate, oval, 10 stamens, shorter than petals. It blooms in October. It is used for ornamental and medicinal purposes. It is distributed in Korea (Jeju, Gyeongnam, Ulleung, Chungnam, Yellow Sea) and Japan. Calligraphy is known as a medicinal herb. The taste is good and the quality is cold. It has the efficacy of detoxifying cyanide and is used for sore throat, inflammation, contusion, skin rash. The outbreak of the panicle reduces the number of blood vessels of acute monocytic leukemia. The outposts contain Portulal, which regulates plant growth. Stems and flowers contain Betacyanin, but mainly contain Betanin and Betanidin, the Aglycone of this component.

As a composition technique using a Sedum oryzifolium Makino extract, Korean Patent Registration No. 10-1326158 entitled " Antihistamine composition " has been disclosed in Korea, and a cosmetic composition capable of exhibiting antioxidant and wrinkle- There is no description of the composition.

Vicia angustifolia var. Segetilis (Thuill.) K. Koch.) Is a vine-grown biennial plant of the dicotyledonous plant rosemary bean and grows in the lower part of the mountain. The stem has a square cross-section and many branching branches at the bottom. It is 60-150cm long and grows sideways. Leaves are alternate, odd-numbered double leaf, 3-7 pairs of small leaves, 2 ~ 3cm long inverted egg shape or wide egg shape, with a small tip and a flat edge. The stipule is divided into two, with serrations on the edge and one line. The flowers bloom in May with a strong reddish purple color and one or two on the axilla. Calyx is divided into 5 pieces, and the split pieces are pointed. The fruit is flat, 3-4cm long, has no hair, and has 10 black seeds. The entire plant is used as feed and the fruit is edible. It has diuretic and detoxifying action and is used for pyogenic skin diseases. It is also good for nosebleeds, menstrual irregularities.

As a composition technique using Vicia angustifolia var. Segetilis (Thuill.) K. Koch. Extract, Korean Patent Registration No. 10-1326158 entitled " Antihistamine composition " and Korean Patent Registration No. 10-0940434 Quot; Sanayaso health beauty preparation method " has been disclosed, and there is no technology for a cosmetic composition that can exhibit antioxidative and wrinkle-reducing effects yet.

( Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima ) is a biennial herbaceous perennial plant of the dicotyledonous plant, which is a biennial plant of the dicotyledonous plant. , Sandy gravel ground, and sunny. It is also called 耳 菜, kwari (耳 耳), papa (婆 婆), wallet (胡甲 菜). The herb has a height of 15 ~ 25cm and branches are diverged and grows diagonally. The leaves resemble the shape of the ear of a rat, with purplish black circles. The edges of the leaves are flat and have hairy hairs. Flowers bloom from April to June in white, bloomed and flowered, and then the end of the flower buds bend down. The fruit is pale yellowish brown with a length of about 9mm. I mean, the point is also the point, the me, the compound word of the herb, the point is small like a point, I mean it is not original but it has similar function and structure, It can be interpreted as meaning that it can be a herb but a plant without it. In this respect, the herb is a weak weed but a persistent vitality and a grass that continues to grow. Herbs are also used in cold, mastitis, dermatitis, boils, etc. because herbs have the effect of lowering fever and sinking swelling. It is a good idea not to eat a lot of people whose body is cold and whose muscles are weak because of taste and quality.

As a composition technique using an extract of Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima , Korean Patent Laid-Open Publication No. 10-2013-0042537, entitled " Antihistamine composition " And there is no description of a cosmetic composition capable of exhibiting the wrinkle-improving effect.

( Fatsia japonica (Thunb.) Decne. & Planch ) is an evergreen shrub of the dicotyledonous plant Araliaceae, also called octagonal gold plate. It grows in the foothills and valleys of the beach. It is 2 ~ 3m in height. Tree bark is ashy-toned white, with several stems growing together and branching. Leaves are alternate, 20 ~ 40cm in diameter, gathered at the ends of the branches. Leaves are 7 ~ 9 pieces, split into palm and dark green, and the forked pieces are egg-shaped and have sawtooth on the edge. It is brown when it is young, but it disappears later. The petiole is 15 ~ 45cm long. The flower is 雜 性 花 (雜 性 花), bloomed in October ~ November, and runs on a large conical flower. When it is young, it is wrapped in white prisoner falling early. There are five petals, stamens, and styles. The fruit is round as a berry, and it melts black in May of the following year. It grows well in the shade, is relatively strong in pollution, and has leaf patterns. It is said that it is good for rheumatism when you put leaf in the bath in the private, It is planted for ornamental purposes and it is said to be a falcon because it is split into 8 pieces. It is distributed in Korea, Japan, and East Asia.

As a composition technique using Fatsia japonica (Thunb.) Decne. & Planch extract, in Korea, Korean Patent Registration No. 10-0663282 entitled " Method for producing extract of falciparum flesh extract for enhancing immune function of domestic falciparum muscle " And there is no technology for a cosmetic composition that can still exhibit antioxidant and wrinkle-reducing effects.

Thus, the present inventors have found that the present inventors have found that the present invention can be applied to a variety of plants such as Suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill.) K. Koch. , Cerastium holosteoides var. Hallaisanense Nakai Mizushima and Fatsia japonica (Thunb.) Decne. & Planch ) exhibit DPPH scavenging activity and collagen production efficacy, and can simultaneously impart antioxidative and anti-wrinkle effects And discovered the fact and completed the present invention.

Korean Patent Publication No. 10-1715622 Korean Patent Publication No. 10-1326158 Korean Patent Publication No. 10-0940434 Korean Patent Publication No. 10-2013-0042537 Korean Patent Registration No. 10-0663282

It is an object of the present invention to provide a cosmetic composition for antioxidation and wrinkle improvement derived from natural products.

It is another object of the present invention to provide a cosmetic comprising the composition.

In order to accomplish the above object, the present invention provides a method for preparing a medicinal herb including Suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill.) K. Koch. A cosmetic composition for improving antioxidative and anti-wrinkle comprising at least one extract selected from the group consisting of Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima and Fatsia japonica (Thunb.) Decne. & Planch do.

In the present invention, the extract is extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof.

In the present invention, the extract is contained in an amount of 0.001 to 20 wt% based on the total weight of the composition.

In the present invention, the extract is characterized by having DPPH scavenging activity and collagen production activity.

The present invention also provides a cosmetic comprising the composition.

Since the extract provided from the present invention is excellent in DPPH scavenging activity and collagen production efficacy, it can be used as a cosmetic composition for antioxidation and wrinkle improvement.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

Throughout this specification, when an element is referred to as " including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

According to one embodiment of the present invention, the present invention relates to a method for the treatment and / or prophylactic treatment of Suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill.) K. Koch. The present invention relates to a cosmetic composition for antioxidation and wrinkle improvement comprising as an active ingredient at least one extract selected from the group consisting of Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima and Fatsia japonica (Thunb.) Decne. & Planch will be.

The extracts according to the present invention can be obtained through various extraction methods known in the art, and specifically from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin and mixtures thereof And may be extracted with a selected solvent.

Specifically, the content of the extracting solvent was determined by the following methods : Suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill.) K. Koch. , Cerastium holosteoides var preferably 2 to 100 times, more preferably 5 to 50 times, most preferably 1 to 100 times the weight of one or more plant solids selected from the group consisting of hallaisanense (Nakai) Mizushima and Fatsia japonica (Thunb.) Decne. & Planch May be 10 to 20 times.

In addition, the above extract can be extracted at room temperature or warmed under conditions that the active ingredient of the extract is not destroyed or the destruction is minimized. The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction and the like can be used depending on the kind of extract and the extraction method.

The filtration is a process of removing suspended solid particles from the extract, and may be performed by filtering particles by using cotton, nylon, paper or the like, or by using an ultrafiltration method, a freezing filtration method, a centrifugal separation method, or the like.

The step of concentrating and then drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a step of pulverizing the finally dried extract may be added.

According to one embodiment of the present invention, the extract may be processed into various forms such as an extract, a powder dried with the extract, and a concentrate obtained by concentrating and filtering the extract.

According to one embodiment of the present invention, the suaeda malacosperma H. Hara , Sedum oryzifolium Makino , Vicia angustifolia var. Segetilis (Thuill. K. Koch.) Obtained from the above- , At least one extract selected from the group consisting of Cerastium holosteoides var. Hallaisanense (Nakai) Mizushima and Fatsia japonica (Thunb.) Decne. & Planch is present in an amount of 0.001 to 20 wt.%, , And more preferably 0.1 to 1% by weight. When the amount of the extract is less than 0.001% by weight based on the total weight of the composition, the function as a composition deteriorates because the effect of improving antioxidation and wrinkle can not be sufficiently obtained. When the extract is more than 20% by weight, It is not economical, and the stability and spreadability of the formulation are lowered and it is difficult to use as a cosmetic product, which is not preferable.

The extract is characterized by having DPPH scavenging activity and collagen production activity. That is, since the antioxidant and wrinkle-reducing effects can be simultaneously given, not only skin beauty but also fundamental skin health improvement can be achieved.

As described above, since the extract has excellent antioxidant and wrinkle-reducing effects, it can be used as a composition for antioxidant and wrinkle-improving.

The antioxidant and wrinkle-reducing composition may be used as a cosmetic composition, a pharmaceutical composition or a food composition, but it is most preferably used as a cosmetic composition.

According to one embodiment of the present invention, when used as the cosmetic composition, it can be used as an external ointment for skin, a cream, a softening longevity, a convergent lotion, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, eye cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, skin lotion, But are not limited to, any of the formulations selected from the group consisting of cleansing foams, cleansing lotions, cleansing creams, cleansing waters, powders, body lotions and body cleansers. The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, .

In the case where the formulation of the present invention is bar diesel, in addition to the above-mentioned effective ingredients, there may be used carbomers, triethanolamine, menthol, ethanol, glycerin, disodium iodide, methylparaben, Can be used. Herein, the carbomer and triethanolamine function as a gradual agent, the menthol functions as a fragrance component, the ethanol functions to give a cooling sensation, the glycerin serves as a moisturizing agent, and the disodium is dithiene, And methylparaben is added for its function as an antiseptic. In addition, the fragrance may include various fragrances such as herb fragrance in addition to menthol fragrance.

In addition to the above-mentioned specific ingredients, it is also within the scope of the present invention to apply the known substances widely used in the art to achieve the functions of the thickener, refreshing feeling, fragrance ingredient, .

When the formulation of the present invention is a powder or a spray (spray), lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be added as a carrier component, Propellants such as carbon, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is added as a carrier component. Examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

According to one embodiment of the present invention, the cosmetic composition for antioxidation and wrinkle improvement may be prepared from a composition such as bar diesel, body lotion, body cream, and body oil for accelerating fat decomposition with addition of usual additives, . ≪ / RTI > In this case, it is preferable that the cosmetic composition is used as a transdermal administration method in which it is directly applied or sprayed on the skin.

According to one embodiment of the present invention, the amount of the cosmetic composition for antioxidation and wrinkle improvement can be appropriately adjusted according to individual differences, form and form such as the age and condition of skin fat, and the composition for antioxidant and wrinkle- . ≪ / RTI >

The composition obtained from the above method exhibits DPPH scavenging activity and collagen production efficacy, and can provide excellent cosmetics for antioxidation and wrinkle improvement.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

<Example 1> Preparation of herb extract

Fifty grams of herbs (Jeju Natural Resources Research Institute) were added to 75% ethanol aqueous solution (1 L) and extracted at 90 ° C for 4 hours. The mixture was filtered through a filter paper, and the above extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered herb extract.

&Lt; Example 2 >

(Cheju Natural Resources Research Institute) was added 1 L to 75% ethanol aqueous solution and extracted at 90 ° C for 4 hours. The mixture was filtered through a filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered watery plant extract.

<Example 3> Preparation of extracts

50 g of ground chrysanthemum (Jeju natural resources research institute) was added 1 L to 75% ethanol aqueous solution and extracted at 90 ° C for 4 hours. The mixture was filtered through a filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a ground powdery extract.

Example 4: Preparation of squid extract

50 g of talc (Jeju Natural Resources Research Institute) was added 1 L to 75% ethanol aqueous solution and extracted at 90 ° C for 4 hours. The mixture was filtered through a filter paper, and the above extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered quail extract.

<Example 5> Preparation of a falcone extract

Fifty grams of FALSONY (Jeju Natural Resources Research Institute) was added to 75% aqueous ethanol solution and 1 L was added and the mixture was extracted at 90 DEG C for 4 hours. The extract was filtered through a filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered falcone extract.

Example 6: Preparation of plant complex extract

(1): 1: 1 (1): 1 (1): 1 (1) 1: 1 : 1: 1, and 50 g of the mixture was added with 1 L of 75% aqueous ethanol solution, and the mixture was extracted at 90 DEG C for 4 hours. The mixture was filtered through filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered plant complex extract.

<Experimental Example 1> Cytotoxicity test: MTT assay

Cytotoxicity experiments were carried out using fibroblasts (HS68 cells) in order to confirm whether the plant extracts of Examples 1 to 6 had irritation to cells.

Human-derived fibroblast cells (HS68 cells) were purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA) containing 10% fetal bovine serum (Gibco BRL, USA) were cultured in 37 ℃, 5% CO ₂ using. The cultured HS68 cells were plated in 96-well plates at 1 × 10 ⁴ cells / mL per well and cultured for 24 hours. After the cultivation, the extracts were cultured for 24 hours by replacing the DMEM medium containing no diluted extracts of each concentration. After 24 hours, CCK-8 (Cell Counting Kit, Dojindo, Japan) solution was added at 1/10 of the cell culture medium, followed by incubation at 37 ° C for 2 hours and then absorbance was measured at 450 nm using a microplate reader . Experiments were carried out three times each, expressed as mean value ± standard deviation, and were calculated using the following equation (1).

[Equation 1]

Cell viability (%) = 100- (absorbance of each sample / control absorbance) x 100

division Cell viability (%) 0 μg / mL 10 μg / mL 20 μg / mL 50 μg / mL 100 μg / mL Example 1 100 101.47 + - 2.41 103.30 ± 3.99 100.95 ± 3.28 100.36 + - 4.34 Example 2 100 98.06 ± 1.06 98.72 ± 0.88 96.84 ± 0.65 104.29 0.30 Example 3 100 90.58 ± 2.05 93.36 ± 3.04 94.77 ± 2.95 102.28 ± 1.97 Example 4 100 99.45 + 4.88 98.63 + - 3.87 100.56 7.68 106.30 ± 3.81 Example 5 100 100.05 + 2.04 21.35 ± 11.59 7.92 ± 0.18 8.44 ± 0.20 Example 6 100 99.05 + 1.94 101.56 ± 2.11 97.52 ± 1.08 98.13 + - 2.32

As a result, as shown in Table 1, it was confirmed that Examples 1 to 6 do not exhibit cytotoxicity and thus can be used as safe materials for cosmetics.

<Experimental Example 2> Antioxidant test: DPPH scavenging activity

Free radical scavenging activity was evaluated in order to examine the antioxidative effect of the plant extracts of Examples 1 to 6.

The free radical scavenging activity was a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303, 1993). The stable free radical DPPH (1,1-diphenyl-2-picryl hydrazyl , Sigma) reagents were used. First, 1 mL of 0.2 mM DPPH solution was diluted with methanol to a suitable concentration, and 2 mL was mixed. After reacting at room temperature for 10 minutes, the absorbance was measured at 517 nm with a microplate reader (Synergy, BioTek, USA). On the other hand, the control group in the free radical scavenging test was measured by the same method except that methanol was added in place of the sample solution. Methanol was added instead of the DPPH solution to obtain a color correction value for each. Experiments were carried out three times each, expressed as mean ± standard deviation, and the free radical scavenging rate was calculated using the following equation (2).

&Quot; (2) &quot;

Free radical scavenging rate (%) = (absorbance of each sample / control absorbance) x 100

division Erase rate (%) 62.5 μg / mL 125 μg / mL 250 μg / mL 500 μg / mL 1000 μg / mL Example 1 2.41 ± 3.14 8.78 ± 0.98 15.47 ± 0.97 25.00 + - 0.68 56.42 ± 1.09 Example 2 10.79 ± 1.15 16.60 ± 1.05 28.62 + - 0.56 45.99 + - 0.82 78.01 0.08 Example 3 11.00 ± 0.83 18.56 ± 0.68 32.03 + - 0.46 54.06 ± 0.16 80.52 + - 0.51 Example 4 11.18 ± 0.65 21.46 ± 0.96 40.94 + 1.90 74.13 ± 2.19 80.28 + - 0.66 Example 5 17.60 ± 2.29 42.88 + - 4.08 74.76 ± 0.88 77.11 + - 2.40 71.80 ± 1.81 Example 6 20.51 ± 1.09 48.65 ± 2.18 75.81 + - 0.55 80.21 + - 0.40 90.11 ± 0.91

As a result, as shown in Table 2, all of the antioxidative effects of Examples 1 to 6 showed good antioxidative effects.

&Lt; Experimental Example 3 > Effect of improving wrinkles: Measurement of collagen production effect

To evaluate the effect of wrinkles on the extracts of Examples 1 to 6, collagen production was measured.

First, cultured fibroblast cells (HS68 cells) were plated in a 24-well plate at 1 × 10 ⁵ cells / mL per well and cultured for 24 hours. After the cultivation, the extracts were cultured for 24 hours by replacing the DMEM medium containing no diluted extracts of each concentration. The amount of collagen synthesis was determined by measuring the amount of procollagen type I C-peptide (Type I C-peptide, PICP) using a collagen-type IC peptide EIA kit (Takara, Japan). The absorbance of the cell culture without treatment of the sample was taken as 100% of the control, and the experiment was carried out three times each, and expressed as the mean value ± standard deviation.

division PIP (%) 0 μg / mL 10 μg / mL 100 μg / mL Example 1 100 - 192.37 ± 13.25 Example 2 100 - 140.15 + 9.43 Example 3 100 - 144.48 ± 11.67 Example 4 100 - 163.37 ± 12.60 Example 5 100 145.12 ± 1.82 - Example 6 100 - 182.55 + - 5.21

As a result, as shown in Table 3, all of Examples 1 to 6 had an excellent collagen-producing effect, but among them, Examples 1 and 5 showed better collagen-producing effect and remarkably improved wrinkle-reducing effect.

<Experimental Example 4> Evaluation of skin wrinkle improving effect and skin irritation evaluation

In order to evaluate the skin wrinkle-improving effect and the skin irritation evaluation, the plant complex extract of Example 6, which has excellent efficacy based on the evaluation results of the above Experimental Examples, was tested. The composition containing the plant complex extract of Example 6 was prepared as a cream type composition according to a conventional manufacturing method as shown in Table 4 below, and the sensory test was conducted using the same.

ingredient Formulation Example
(weight%) Comparative Formulation Example
(weight%) One The plant complex extract of Example 6 5.0 - 2 Cetearyl alcohol 1.0 1.0 3 Glyceryl stearate / phage-100 stearate 1.0 1.0 4 Polysorbate 60 1.0 1.0 5 Sorbitan sesquioleate 0.3 0.3 6 Cetyl octanoate 6.0 6.0 7 Squalane 8.0 8.0 8  Apricode kernel oil 4.0 4.0 9 Dimethicone 2.0 2.0 10 glycerin 5.0 5.0 11 Magnesium aluminum silicate 0.4 0.4 12 Xanthan gum 0.03 0.03 13 Preservatives and incense a very small amount a very small amount 14 Purified water (to 100) to 100 to 100

First, 20 women over 20 years of age were given cream (test group) containing the composition on the left side of the face and creams (control group) containing no composition on the right side of the face twice daily for 12 weeks . The sensory test was evaluated relative to the effect of the test cream on the basis of the control cream, and the evaluation of the skin irritation was evaluated on the skin such as itching, stinging and erythema. The evaluation was carried out according to the blotting law standards of excellent (5 points), excellent (4 points), normal (3 points), poor (2 points) and very poor (1 point).

number Skin irritation Wrinkle improvement effect Formulation Example Comparative Formulation Example Formulation Example Comparative Formulation Example One 4 4 4 5 2 5 4 5 5 3 4 4 5 5 4 5 5 4 4 5 4 4 5 4 6 5 5 5 4 7 4 4 4 4 8 5 4 5 4 9 4 4 4 5 10 5 4 5 5 11 4 5 4 4 12 5 5 5 5 13 5 5 5 4 14 5 5 5 5 15 4 4 4 4 16 5 5 5 5 17 5 4 5 4 18 5 5 5 4 19 4 4 4 3 20 4 4 4 4 Average 4.55 4.40 4.60 4.35

As a result, as shown in Table 5, it was confirmed that the formulation example was superior in skin safety due to a low degree of skin irritation as compared with the comparative formulation and had better wrinkle-reducing effect.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

( Hokkaido ), Suaeda malacosperma H. Hara , Sedum oryzifolium Makino, Vicia angustifolia var. Segetilis (Thuill.) K. Koch. , Cerastium holosteoides var hallaisanense (Nakai) Mizushima and A cosmetic composition for antioxidant and wrinkle improvement comprising an extract of a plant complex of Fatsia japonica (Thunb.) Decne. & Planch as an active ingredient.
The cosmetic composition according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof.
The cosmetic composition for antioxidant and wrinkle improvement according to claim 1, wherein the extract is contained in an amount of 0.001 to 20 wt% based on the total weight of the composition.
The cosmetic composition for antioxidant and wrinkle improvement according to claim 1, wherein the extract has a DPPH scavenging activity and a collagen production activity.
A cosmetic comprising the composition of any one of claims 1 to 4.