KR102557372B1 - Composition for Preventing or Treating Hair Loss or Stimulating Hair Sprouting or Hair Growth Comprising Extracts of Rosa davurica Pall as Active Ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing or treating hair loss, or for promoting hair growth or hair growth, comprising an extract of wild chives as an active ingredient.
The present invention has no cytotoxicity, promotes cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT), and promotes hair growth by increasing hair thickness and density clinically in animal hair loss models And, since it has an excellent effect in significantly increasing the number of hair follicles and bulb size, it can be usefully used for the purpose of preventing or treating hair loss or promoting hair growth or hair growth in the fields of medicine, food, and cosmetics.

Description

Composition for Preventing or Treating Hair Loss or Stimulating Hair Sprouting or Hair Growth Comprising Extracts of Rosa davurica Pall as Active Ingredient}

The present invention relates to a composition for preventing or treating hair loss, or for promoting hair growth or hair growth, comprising an extract of wild chives as an active ingredient.

In the human body, hair grows from hair follicles in the skin, and the hair follicle from which hair grows is a complex organ composed of inner root epithelial sheath, outer root epithelial sheath, hair stem, and hair matrix, and has cycles such as growth, catagen, and telogen throughout life. It is characterized by constant recurrence.

In particular, among the cells forming the hair follicle, dermal papilla cells play an important role in the growth of hair, and proliferation of constituent cells of the hair follicle, including keratinocytes of the matrix surrounding the dermal papilla, occurs during the growth phase of the hair follicle. In the catagen phase of the hair follicle, the growth of the hair follicle stops, apoptosis, condensation of the dermal papilla, and reorganization of the extracellular matrix occur. only remains

Hair loss refers to an increase in the number of hairs that fall out abnormally because the ratio of hairs growing from hair follicles in the catagen phase or telogen phase is higher than that of hairs growing in the follicles in the growth phase during the growth cycle of the hair follicle. Recently, as the tendency to emphasize appearance is becoming more prominent, interest in hair loss is also increasing. The causes of such hair loss have been discussed, such as poor blood circulation, excessive action of male hormones, excessive sebum secretion, reduced scalp function due to peroxides and bacteria, genetic factors, aging, stress, etc. The cause is unknown.

In general, the most typical form of hair loss is androgenetic alopecia, which is caused by excessive action of male hormones, and it occurs dependently on androgen, a male hormone. It is known. Testosterone is converted to DHT by 5-reductase, and this conversion occurs by Type 2 5-alpha-reductase among the two types of 5-alpha-reductase, Type 1 and Type 2. It has been reported that gradual miniaturization of dermal papillae and hair follicles is progressed by converted DHT.

In the treatment and prevention of such alopecia, the most widely used currently is minoxidil-containing preparations from Upjohn, approved by the US FDA, and recently, finasteride (finasteride), which has an inhibitory effect on type 2 5-alpha-reductase, from Merck. ) is the main ingredient, Propecia, has been released and used. In addition, preparations containing trichosaccharide from Crinos, Co. as a main component are used.

In addition, studies based on natural extracts have been conducted, and extracts such as Cnidium (Patent No. 2010-0119761), Sanghwang mushroom (Patent No. 2012-0038426), Goldfinch (Patent No. 2012-0038426), and chamomile (Patent No. 2012-0038426). 2012-0015874, Patent No. 2010-0091571), Hasuo (Patent No. 2013-0021464, Patent No. 2011-0097386), Iris (Patent No. 2012-0115980), Pomegranate (Patent No. 2012-7012465), There are results such as ginkgo leaf extract (Patent No. 2006-7008793). However, when these hair restorers or hair restorers are used clinically, the effect on the scalp is not so outstanding, and they are more effective than Minoxidil or Propecia, which are reluctant to use due to concerns about side effects, and hair restorers made of natural substances with fewer side effects. Exploratory research is actively underway.

Under the above background, the present inventors have made intensive research efforts to utilize for the prevention or treatment of hair loss symptoms. In a hair loss model, hair growth is promoted by increasing the thickness and density of hair clinically, and the number of hair follicles and bulb size are also significantly increased, confirming that there is an excellent effect in preventing or treating diseases such as alopecia, and completing the present invention. It became.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for preventing or treating hair loss, or for promoting hair growth or hair growth, comprising an extract of genus japonica as an active ingredient; A cosmetic composition for improving hair loss, or for promoting hair growth or hair growth; And to provide a food composition for improving hair loss, or for promoting hair growth or hair growth.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating hair loss, or for promoting hair growth or hair growth, comprising an extract of Raisinus sinensis as an active ingredient.

The present inventors have made intensive research efforts to discover naturally derived ingredients that can effectively prevent hair loss or promote hair growth or hair growth. As a result, it was confirmed that the extract of the wild rhizome effectively promotes hair growth and hair growth and prevents hair loss.

" Rosa davurica Pall." of the present invention means a deciduous shrub of the dicotyledonous plant Rosaceae Rosaceae. In a preferred embodiment of the present invention, the extract of the wild chives contains a large amount of Quercetin, Kaempferol 3,4-di-O-glucoside, Ellagic acid, and Apigenin. have confirmed that

As used in the present invention, the term "extract" refers to an extract obtained by the extraction treatment of a raw heathen tree, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a refined or purified product of the extract, or a mixture thereof, etc. , including the extract itself and extracts of all formulations that can be formed using the extract. The extract of the present invention may be preferably prepared and used in the form of a dry powder after extraction. In the extraction of the fresh wild cypress tree, the method for extracting the extract is not particularly limited, and can be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination with two or more extraction methods.

In the present invention, the type of extraction solvent used for extracting the natural heat guava tree is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more.

In the present invention, the extract obtained by hot water extraction or cold brewing is filtered to remove floating solid particles by filtering out the particles using, for example, nylon or by using cryofiltration, and then used as it is or freeze-dried, hot-air-dried, It can be used after being dried using spray drying or the like.

As used herein, the term "fraction" refers to a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

A non-limiting example of the fractionation method may be a method of obtaining a fraction from the extract by treating the extract obtained by extracting the extract from the extract with a predetermined solvent. In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohol is used among the fractionation solvents, C1 to C4 alcohols may be specifically used.

The extract of the wild plants of the present invention can be extracted from any one or more parts of the whole plant, leaves, stems, roots, and fruits of the plants. In addition, the extract of the wild plant extract can be extracted from natural, hybrid or mutant plants, and can also be extracted from plant tissue culture.

As used herein, the term "hair loss" refers to a phenomenon in which hair completely comes out of the scalp. In addition, the term “hair growth” in the present specification means that hair grows on the scalp, and the term “hair growth” in the present specification means that the length of the hair becomes longer and has the same meaning as “wool”, another term used in the art. is used as As demonstrated in the following examples, the extract of the present invention promotes cell proliferation of hair papilla cells (HHDPC) (see FIG. 2) and promotes cell proliferation of keratinocytes (HaCaT) (see FIG. 3). ), it was confirmed that hair growth was promoted by increasing hair thickness and density clinically in an animal hair loss model (FIG. 4), and the number of hair follicles and bulb size were also significantly increased (FIG. 5).

In the pharmaceutical composition for preventing or treating hair loss, or for promoting hair growth or hair growth of the present invention, the extract of the wild chive tree promotes cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT).

Therefore, the composition of the present invention can be applied as a multifaceted and stable hair loss prevention or treatment, or an effective hair loss prevention or treatment composition exhibiting hair growth and hair growth promoting effects, or a hair growth / hair growth composition.

According to the present invention, the composition of the present invention is used for the treatment of alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, hereditary hypotrichosis simplex, localized alopecia. (circumscribed alopecia), alopecia congenitalis, alopecia pubis, seborrheic alopecia (alopecia seborrheica), senile alopecia (alopecia senilis), alopecia totalis, alopecia universalis, and telogen effluvium It can be used to treat alopecia of any one selected from (telogen effluvium).

When the composition of the present invention is prepared as a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used in formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, including, but not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil; it is not going to be The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components. Suitable pharmaceutically acceptable carriers and agents are described in detail in Remington's Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention may be administered orally or parenterally, and according to one embodiment of the present invention, it is administered in a parenteral manner, and according to another embodiment of the present invention, it is administered in a transdermal manner.

A suitable dosage of the pharmaceutical composition of the present invention is variously prescribed by factors such as formulation method, administration method, patient's age, weight, sex, medical condition, food, administration time, administration route, excretion rate and response sensitivity. It can be. A preferred dosage of the pharmaceutical composition of the present invention is within the range of 0.0001-100 mg/kg for adults.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulation using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily performed by those skilled in the art. or it may be prepared by incorporating into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, syrup or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, powder, granule, tablet or capsule, and may additionally contain a dispersing agent or stabilizer.

According to another aspect of the present invention, the present invention provides a food composition for improving hair loss, or for promoting hair growth or hair growth, comprising an extract of Raisinus sinensis as an active ingredient.

The wild chive extract of the present invention has no cytotoxicity, promotes cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT), and increases hair thickness and density clinically in animal hair loss models to promote hair growth It can be usefully used as a food composition for preventing hair loss or promoting hair growth or hair growth because it has the effect of promoting and significantly increasing the number of hair follicles and bulb size. The effects of the extract of the present invention on the prevention of hair loss, or the promotion of hair growth or hair growth are as described above. The food composition may be used in the form of health functional food, but is not limited thereto.

The food composition of the present invention may be included in the form of a fraction or a processed product of the extract of Saengyeolgi tree. In addition, the composition may include a food additive that is acceptable in food science in addition to the active ingredient.

In the present invention, "food supplement additive" refers to a component that can be added to food supplementally, and can be appropriately selected and used by those skilled in the art as being added to prepare a health functional food of each formulation. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners , pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.

The food composition of the present invention may include health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, "functionality" means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions, and specifically means preventing hair loss, or promoting hair growth or hair growth. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation.

In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of dosage forms, and unlike general medicines, it has the advantage of not having side effects that can occur when taking medicines for a long time by using the edible raw extract of wild chives as a raw material. It promotes cell proliferation of (HHDPC) and keratinocytes (HaCaT), promotes hair growth by increasing hair thickness and density clinically in animal hair loss models, and has the effect of significantly increasing the number of hair follicles and bulb size, It can be taken as an adjuvant to enhance the therapeutic effect of alopecia.

There is no limit to the form that the health functional food of the present invention can take, and it may include all foods in a conventional sense, and may be used interchangeably with terms known in the art, such as functional foods. In addition, the health functional food of the present invention may be prepared by mixing suitable other auxiliary ingredients and known additives that may be included in food according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes, etc., and it can be prepared by adding to juice, tea, jelly, juice, etc. prepared by using the extract of the wild rhizome according to the present invention as a main component. Also included are foods used as feed for animals.

According to another aspect of the present invention, the present invention provides a cosmetic composition for improving hair loss, or for promoting hair growth or hair growth, comprising an extract of Raisinus sinensis as an active ingredient.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the extract of the wild chives, for example, antioxidants, stabilizers, solubilizers, common adjuvants such as vitamins, pigments and fragrances, and carriers. includes

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is a surfactant-containing cleanser, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Since the cosmetic composition of the present invention has the above-mentioned pharmaceutical composition and active ingredients and uses in common, the description of the common information in relation to the pharmaceutical composition is omitted in order to avoid excessive complexity of the present specification.

The features and advantages of the present invention are summarized as follows:

(1) The present invention provides a composition for preventing or treating hair loss, or for promoting hair growth or hair growth, comprising an extract of wild genus japonica as an active ingredient.

(2) The fresh wild chive extract of the present invention has no cytotoxicity, promotes cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT), and increases hair thickness and density clinically in animal hair loss models. Since it has an excellent effect on promoting hair growth and significantly increasing the number of hair follicles and bulb size, it can be usefully used for the purpose of preventing or treating hair loss or promoting hair growth or hair growth in the fields of medicine, food, and cosmetics.

Figure 1 is a graph of the results of measuring the active ingredients of the extract of wild genus japonica using HPLC (from the top, standard (A), outpost (B), leaf (C), stem (D), root (E), and fruit (F), Peak 1 is quercetin, peak 2 is kaempferol, and peak 3 is the peak for ellagic acid).
Figure 2 is a graph showing changes in cell proliferation of hair papilla cells (HHDPC) according to the treatment with the extract of wild genus japonica (A sheath, B leaf, C stem, D root, and E fruit).
Figure 3 is a graph showing changes in cell proliferation of keratinocytes (HaCaT) according to the treatment of extracts of wild genus japonica (A sheath, B leaves, C stems, D roots, and E fruits).
Figure 4 is a photograph taken by comparing the change in thickness and density of hair after applying the extract of wild genus japonica in an animal hair loss model.
Figure 5 is a photograph of histological changes after applying the extract of the wild plant in an animal hair loss model.

Hereinafter, the configuration of the present invention will be described in more detail with examples, but the scope of the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms different from each other, only the present embodiments It is provided to make the disclosure of the invention complete, and to fully inform those skilled in the art of the scope of the invention to which the present invention belongs, and the present invention is only defined by the scope of the claims. In addition, the significance between the control group, comparison group, and experimental group according to the experimental results described below was verified by One-Way ANOVA.

Example 1. Preparation of extracts of fresh lentil trees

Preparation Example 1-1. Preparation of extracts from wild plants

After removing impurities by washing the outposts of the wild plants (origin: domestic, place of purchase: Wonsam Rosehip Co., Ltd.) in running water, 1L of 30% ethanol aqueous solution was added to 100 g of the plants, stirred at room temperature for 1 day, and then stirred for 1 hour. Extracted by sonication. The process was carried out in 3-batch. After collecting the obtained extract, concentrated under reduced pressure at 40 ℃ or less, and then lyophilized to prepare a powder form (Preparation Example 1).

Preparation Example 1-2. Manufacture of extracts of wild genus japonica leaves

Except for the use of the leaves of the wild cypress tree, the extract of the leaf extract of the cypress tree was prepared in the same manner as in Preparation Example 1-1.

Preparation Example 1-3. Manufacture of the extract of the stem of the wild apricot tree

Except for using the stem of Cyperus japonica, the extract of the stem of Cyperus japonica was prepared in the same manner as in Preparation Example 1-1.

Preparation Example 1-4. Preparation of the extract of the root of the wild apricot tree

Except for the use of the root of Cyperus japonica, the extract of the root of Cyperus japonica was prepared in the same manner as in Preparation Example 1-1.

Preparation Example 1-5. Preparation of extracts from the fruit of the wild apricot tree

A fruit extract of the fruit of the fruit of the wild plant was prepared in the same manner as in Preparation Example 1-1, except that the fruit of the plant of the wild plant was used.

Example 2. Component analysis of the extract of fresh genus japonica

The content of the indicator component of the extracts of the wild genus japonica prepared in Example 1 was compared and analyzed.

High performance liquid chromatography analysis was performed using Thermo UHPLC U3000. HPLC analysis conditions were as follows. A Sunfire TM C18 column (Waters, 250 × 4.6 mm, 5 μm) was used as the column, and the column temperature was set at 25°C. The UV wavelength was measured at 270 nm, and water containing 0.1% formic acid and acetonitrile (gradient mode) were used as the mobile phase. [0040] The extracts of the wild rhododendron sinensis prepared in Example 1 (Preparation Examples 1-1 to 1-5) were dissolved in a mobile phase solvent at a concentration of 5 mg/ml, and 10 uL was injected, followed by measurement at a flow rate of 1 ml/min. The content of the marker component of the extract is shown in [Table 1].

[Table 1]

Referring to [Table 1] and [Fig. 1], the outposts of the wild plants are Quercetin, Kaempferol 3,4-di-O-glucoside, Ellagic acid and Apigenin ) was analyzed to contain a large amount.

In addition, unlike other parts, it was analyzed that the leaves of the wild cypress tree contain a large amount of quercetin and kaempferol (Kaempferol 3,4-di-O-glucoside), and the roots of the cypress tree contain a large amount of apigenin. .

Experimental Example 1. Evaluation of hair growth efficacy through analysis of cell proliferation activity of hair papilla cells (HHDPC)

In order to confirm the hair growth stimulating effect of the fresh wild chive tree extract (Preparation Examples 1-1 to 1-5) prepared in Example 1, hair papilla cells of hair follicles involved in hair production (human hair dermal papilla cells, HHDPC) (Sciencell, cat. no. 2400) was analyzed to evaluate the hair growth efficacy of the extracts of the genus Cyperus japonica.

In the experiment, all papilla cells were inoculated into a 96-well plate at a concentration of 0.25 X 10 ⁴ cells/ml in MSC (Mesenchymal Stem Cell) medium containing 10% FBS (fetal bovine serum, Cambrex), and cultured for 24 hours at 37 °C. ℃, 5% CO ₂ It was cultured under humid conditions.

The extracts from Preparative Examples 1-1 to 1-5 were treated at concentrations of 0.01, 0.1, and 1 μM, respectively, and incubated for 24, 48, and 72 hours, followed by 5 mg/ml MTT [3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] 20 μl of the reagent was treated with the culture solution, and incubated for 4 hours at 37° C. under 5% CO ₂ humid conditions.

For comparison experiments, a test group treated with only 200 μl of solvent was used as a negative control group, and a test group treated with 0.08 μg/ml of finasteride (Merck) was used as a positive control group.

During cultivation, living cells produce formazan, a purple crystal. After dissolving the produced formazan through DMSO treatment, the degree of cell growth was measured by measuring absorbance at 560 nm using a microplate reader. The test was repeated three times, and the experimental results are summarized in FIG. 2 .

Referring to FIG. 2, in the experimental group treated with the raw heathen tree extract, it was observed that the proliferation of human hair papilla cells was promoted. Improved cell proliferation was observed, and improved cell proliferation was also observed compared to the finasteride-treated group, which is a positive control group.

In addition, all the extracts from each part of the wild plant extracts effectively promoted the proliferation of human hair papilla cells, and as the extracts from all parts were treated, the proliferation of hair papilla cells was remarkably improved compared to the negative control group.

Through the above experimental results, it was confirmed that the extract of the present invention can be effectively used for preventing or treating diseases such as alopecia by promoting the growth of hair papilla cells (see FIG. 2).

Experimental Example 2. Evaluation of the effect on the growth of human keratinocytes (HaCaT cell)

In order to confirm the hair growth promoting effect of the extract prepared in Example 1, the cell proliferation activity of keratinocytes (HaCaT), which are constitutive cells of hair, was analyzed to evaluate the hair growth efficacy of the extract.

The experiment was performed as described above, except that keratinocytes (HaCaT) (Dr. N. E. Fuseing, Heidelberg, Germany) were used instead of hair papilla cells and RPMI medium containing 10% FBS (fetal bovine serum, Cambrex) was used. It was evaluated in the same way as Experimental Example 1, and the experimental results are summarized in FIG. 3.

Referring to FIG. 3, in the experimental group treated with the raw heathen tree extract, it was observed that the proliferation of keratinocytes (HaCaT) was promoted. 95% (0.01 μM), 110% (0.1 μM), and 120% (1 μM) improved cell proliferation were observed, respectively, and 95% (0.01 μM), 110% (0.1 μM), respectively, compared to the positive control finasteride-treated group. μM), and 125% (1 μM) improved cell proliferation was observed.

In addition, all the extracts from each part of the wild chive tree effectively promoted the proliferation of keratinocytes (HaCaT). ), 115% (0.1 μM), and 110% (1 μM) improved cell proliferation was observed, and the fresh radishes stem extract (Preparation Example 1-3) was 90% (0.01 μM) and 95%, respectively, compared to the negative control. % (0.1 μM), and 100% (1 μM) improved cell proliferation was observed, and the extracts from the roots of wild chives (Preparation Examples 1-4) were 90% (0.01 μM) and 110% (0.1 μM) compared to the negative control, respectively. μM), and 105% (1μM) improved cell proliferation was observed, and the extracts of the fruit extracts (Preparation Examples 1-5) were 100% (0.01μM), 105% (0.1μM), 105% (0.1μM), and 115% (1 μM) improved cell proliferation was observed.

Through the above experimental results, it was confirmed that the extract of the present invention can be effectively used for preventing or treating diseases such as alopecia by promoting the growth of keratinocytes (HaCaT) (see FIG. 3).

Experimental Example 3. Evaluation of hair growth in an animal hair loss model (C57BL/6 mouse)

In order to visually evaluate the hair growth promoting efficacy of the previously verified extract of wild genus japonica, it was applied to an animal hair loss model.

As the hair loss model applied in this experiment, the C57BL/6 mouse hair loss model, which is most commonly applied in various studies and experiments related to hair loss, was adopted.

Hair removal cream is applied to the dorsal hair of a 7-week-old mouse, and hair is removed at the same time as hair is induced to the anagen phase. way you can For reference, the skin color change of a typical C57BL/6 mouse starts to change from gray to black as it enters anagen stage 5 on day 9, and from day 14 to day 16 is anagen stage 6, which is the period in which hair growth proceeds most actively.

For the experiment, the hair on the back of 7-week-old C57BL/6 mice was removed, and mice with clean back skin were divided into 3 groups (groups A-C) of 5 mice each. After destroying hair follicles in all groups using the Wax-Rosin mixture, Group A was orally administered with saline for 18 days (negative control group), and Group B was administered Propecia (finasteride) developed by Merck at 0.5 mg/day. It was orally administered for 18 days at a dose of kg (positive control group), and group C was orally administered for 18 days at a dose of 5 mg / kg by dissolving the extract (Preparation Example 1-1) in saline. Pictures taken of the degree of hair distribution at the time of 12 days after administration are summarized in FIG. 4 .

Referring to FIG. 4, it was confirmed that the skin color of the mouse treated with the extract of the present invention turned black in a short time, the hair grew quickly, and the number of hair follicles significantly increased in the actual skin tissue and maintained the normal hair shape. Compared to the control group, it showed an effect of promoting hair growth for about 3 days.

In addition, it was confirmed that the hair growth period of the group applied with the extract of the present invention was accelerated by about 1 day compared to the group applied with finasteride, which is a positive control group.

In summary, stimulating the hair growth of mice means that the extract of the wild chives has a significant effect on the promotion of the hair growth and the maintenance of the growing period, and through the above results, the extract of the hair growth of the present invention is effective in promoting hair growth. It was confirmed clinically that

Experimental Example 4. Histological observation evaluation

After induction of anagen through hair removal in the C57BL/6 mouse hair loss model analyzed in Experimental Example 3, changes in hair follicles, skin thickness, and tissue in the skin to which the fresh genus japonica extract was applied were observed.

Specifically, mice in each group were killed on day 21 in the C57BL/6 mouse hair loss model, and then tissues of the hair-growth region of the back skin were collected and fixed with a 4% formaldehyde solution. After fixation, water washing and dehydration were carried out, embedding in paraffin, and 7 μm sections were prepared, stained with Hematoxylin & Eosin (H&E) method, and observed under a microscope.

As a result of the observation, it was confirmed that the length of the hair follicles was increased by the treatment with the extract of the genus japonica extract, showing the shape of the hair follicles in the growth phase, and the number of hair follicles and the size of the bulb significantly increased (see FIG. 5).

In addition, it was confirmed that the ratio of ‘anagen hair/telogen hair’ increased by 3.5 times.

Through the above results, it was confirmed that the extract of the present invention increases the number of hair follicles and bulb size, and increases the ratio of anagen hair to be effective in promoting hair growth and preventing hair loss.

Having described specific parts of the present invention in detail above, it is clear that these specific techniques are merely preferred embodiments for those skilled in the art, and the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (6)

Including the extract of the wild cypress tree as an active ingredient,
The extract of the wild cypress tree is an extract obtained by extracting ethanol as a solvent from the outpost part of the cypress tree,
The fresh fever lily extract contains Quercetin, Kaempferol 3,4-di-O-glucoside, Ellagic acid and Apigenin components,
The extract of the raw heathen tree is characterized in that it promotes the cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT),
Characterized in that the extract of the wild genus chinensis increases the number of hair follicles and the size of the bulb, and increases the ratio of anagen hair,
A pharmaceutical composition for preventing or treating hair loss, or for promoting hair growth or hair growth. delete delete According to claim 1,
The composition is effective against alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, hereditary hypotrichosis simplex, circumscribed alopecia, and congenital alopecia. (alopecia congenitalis), alopecia pubis, seborrheic alopecia (alopecia seborrheica), senile alopecia (alopecia senilis), alopecia totalis, alopecia universalis, and telogen effluvium. Characterized in treating one alopecia,
A pharmaceutical composition for preventing or treating hair loss, or for promoting hair growth or hair growth. Including the extract of the wild cypress tree as an active ingredient,
The extract of the wild cypress tree is an extract obtained by extracting ethanol as a solvent from the outpost part of the cypress tree,
The fresh fever lily extract contains Quercetin, Kaempferol 3,4-di-O-glucoside, Ellagic acid and Apigenin components,
The raw heat chive extract is characterized in that it promotes the cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT),
Characterized in that the extract of the wild genus chinensis increases the number of hair follicles and the size of the bulb, and increases the ratio of anagen hair,
A food composition for preventing hair loss, or for promoting hair growth or hair growth. Including the extract of the wild cypress tree as an active ingredient,
The extract of the wild cypress tree is an extract obtained by extracting ethanol as a solvent from the outpost part of the cypress tree,
The fresh fever lily extract contains Quercetin, Kaempferol 3,4-di-O-glucoside, Ellagic acid and Apigenin components,
The raw heat chive extract is characterized in that it promotes the cell proliferation of hair papilla cells (HHDPC) and keratinocytes (HaCaT),
Characterized in that the extract of the wild genus chinensis increases the number of hair follicles and the size of the bulb, and increases the ratio of anagen hair,
A cosmetic composition for preventing hair loss, or for promoting hair growth or hair growth.