KR101434300B1 - Cosmetic Composition Comprising Vitis flexuosa Thunb, Penthorum chinense PURSH Extract for Skin Whitening - Google Patents

Abstract

The present invention provides a whitening cosmetic composition comprising a plant extract of Vitis flexuosa Thunb or Penthorum chinense PURSH as an active ingredient. The cosmetic composition of the present invention suppresses the enzyme tyrosinase, which is an enzyme important for the mechanism of melanin production, and suppresses the amount of melanin production, thereby exhibiting a remarkable whitening effect.

Description

(Cosmetic Composition Comprising Vitis flexuosa Thunb, Penthorum chinense PURSH Extract for Skin Whitening)

The present invention relates to a plant extract having a whitening activity and a whitening cosmetic composition containing the same. More particularly, the present invention relates to a plant extract having a whitening activity, , And an effective ingredient having a high melanin inhibitory effect. The cosmetic composition is prepared by adding, to the cosmetic base, an extract of at least one kind selected from the group consisting of the bird head and the octopus legs alone or in an amount of 0.00001 to 30% And has a whitening activity remarkably exhibiting a whitening effect, and a whitening cosmetic composition containing the same.

The color expressed in human skin, hair, and eyes is determined by melanin, carotene, and hemoglobin. Especially, the most important factor that determines the color of skin is melanin. Skin color depends on the amount and nature of melanin and the degree of distribution. do.

The main function of melanin is to protect the skin from damage by harmful radicals by removing these harmful radicals. Thus, a high level of melanin means that it has an effective countermeasure to protect the skin from physical and chemical toxicants. Factors that promote the production of melanin include not only sunlight (ultraviolet rays) but also hormones such as estrogen, prostaglandine and the like.

Melanin is produced by sunlight (ultraviolet rays) in melanocytes after tyrosine is converted to dopa and dopa chrome by an enzyme called tyrosinase and then through complex oxidation and condensation. The produced melanin is transferred to the skin cells and melanin is lost with epidermal detachment, and it is circulated to disappear. The mechanism of melanin production is characterized by the fact that only one enzyme called tyrosinase is involved. Therefore, whitening effect can be expected by inhibiting tyrosinase activity and preventing melanin production.

The method of inhibiting melanin production in whitening cosmetics can be largely divided as follows. The first method is to block and remove ultraviolet light which is a main cause of melanin production, and this method can effectively inhibit melanin production by containing a light scattering agent or a light blocking agent in a cosmetic composition. The second way is to inhibit the production of melanin by inhibiting the synthesis of core carbohydrates required for tyrosinase activity such as glucosamine. The third method is to interfere with the function of tyrosinase involved in melanogenesis, such as enzymatic acid (kojic acid) or arbutin. The fourth method has specific toxicity to melanocytes, such as hydroquinone, which produces melanin, which interferes with cell division. The fifth method is to expect a whitening effect by exerting a strong antioxidative effect while tyrosine, such as vitamin C, undergoes complex oxidation and condensation reaction through dopa and dopachrome.

In recent years, whitening cosmetics, which are considered to be most ideal, firstly block ultraviolet rays, eliminate radicals generated in the body, inhibit the production of inflammatory substances that may be caused by ultraviolet rays, and ultimately inhibit melanin biosynthesis It is known to use complex materials.

However, when a large amount of natural plants are used, it may cause side effects such as irritation to the skin to cause skin pigmentation, and thus it is required to develop a substance and a method capable of maximizing the whitening effect even at a low concentration have.

On the other hand, Korean Patent Registration No. 230130 discloses a whitening cosmetic composition containing an upper body extract, and Korean Patent Application No. 1999-17810 discloses a whitening cosmetic composition containing an upper body extract and vitamin C. However, the whitening agents developed to date have a weak whitening effect, and they have a problem of improving side effects such as skin irritation.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors have made intensive researches to develop a whitening cosmetic composition having a high whitening effect and a low skin irritancy as compared with conventional whitening cosmetics. As a result, it has been found that a plant extract of Vitis flexuosa Thunb or Penthorum chinense PURSH When used, inhibits tyrosinase enzyme which is an important enzyme for the melanin production mechanism and inhibits melanin production to exhibit a whitening effect, thus completing the present invention.

It is therefore an object of the present invention to provide a whitening cosmetic composition comprising a plant extract of Vitis flexuosa Thunb or Penthorum chinense PURSH.

Another object of the present invention is to provide a whitening active food composition or a pharmaceutical composition containing the plant extract.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The present invention provides a whitening cosmetic composition.

The present inventors have made intensive researches to develop a whitening cosmetic composition having a high whitening effect and a low skin irritancy as compared with conventional whitening cosmetics. As a result, it has been found that a plant extract of Vitis flexuosa Thunb or Penthorum chinense PURSH It was confirmed that tyrosinase enzyme, which is an important enzyme for melanogenesis, is inhibited and melanin production is suppressed to show whitening effect.

According to one aspect of the present invention, there is provided a whitening cosmetic composition comprising, as an active ingredient, a plant extract of Vitis flexuosa Thunb or Penthorum chinense PURSH.

Vitis flexuosa Thunb is a vine plant that grows over 3 m in length and has no hairs on stems or branches. It is a plant that grows up with its tendrils wrapped around objects. The flowers bloom in June with yellowish green, and the leaves face each other. Sometimes peduncle develops tendrils with 5 petals and upper part attached. The fruit is ripe in September and has 2-3 seeds, about 7 mm in diameter. It is a deciduous broad-leaved tree native to Hwanghae-do, Gangwon-do and Jeju-do. It is distributed in Japan, China and Taiwan. It adapts well in both sunny and shaded areas and has a good growth in fertile green areas. It is resistant to cold weather and grows well in urban areas and beaches.

Vitis flexuosa Thunb is effective against hypoglycemic effect, neuroprotective effect, and skin pain caused by hormone imbalance, and thus it can be used as a medicine and health functional food, but there is no report that it can be used as a whitening cosmetic product.

Penthorum chinense PURSH (Penthorum chinense PURSH) is a fleshy, perennial plant that grows in wetlands such as ducks and ditches with perennial plants of dicotyledonous rosewood. The flower blooms in yellowish-white color in July-August, and the branch is divided into four branches like octopus legs at the end of the stem. The diameter of the flower is 4-5 mm, there is no petal, and the calyx is cup-shaped and is divided into 5 pieces. Fruits are seeds 6-7 mm in diameter, and have five ovary bottoms. When they are multi-colored, the top part of the attached part is split and comes out.

It is distributed in Korea, Japan, China, etc., and the root is used as a medicine. It is a plant known to be effective when it is applied to the swollen waters.

Octmental bridge (Penthorum chinense PURSH) is known to prevent arterial atherosclerosis and is effective for vascular disease and liver disease, but there are no reports on other functions.

According to a preferred embodiment of the present invention, the plant is preferably selected from the group comprising plants flowers, leaves, stems, fruits, seeds, roots and outposts, more preferably seeds, Fruit, root and toppings, and most preferably may be selected from the group comprising the stem, seeds and outpost of the plant. The reason for the fact that the most preferable extraction site of the plant is a stem, a seed or an outpost in the present specification is not clearly recognized to the public as to the efficacy of the plant. However, the stem, seed or outpost of the plant is whitened Because it proved to be excellent in effect.

According to a preferred embodiment of the present invention, the plant extract can be obtained by using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or hydrated lower alcohol (D) acetone, (e) ethyl acetate, (f) chloroform, (g) methylene chloride, or (h) 1 (methyl ethyl ketone) , And 3-butylene glycol as an extraction solvent. More preferably, extraction is carried out using methanol and an aqueous solution of ethanol, and most preferably extraction is carried out using an aqueous methanol solution.

According to a preferred embodiment of the present invention, the amount of the plant extract is preferably 0.00002-30% by weight, more preferably 0.0001-0.1% by weight, and most preferably 0.0025-0.01% by weight based on the total weight of the whitening cosmetic composition .

In the composition of the present invention, the most preferable amount of the plant extract is 0.0025-0.01% by weight. When the content of the plant extract is less than 0.0025% by weight, the effect of the invention as a skin whitening cosmetic can not be sufficiently obtained And if it exceeds 0.01% by weight, there is a problem in safety due to cytotoxicity.

According to a preferred embodiment of the present invention, the plant extract exhibiting whitening activity is preferably contained in a composition selected from the group comprising cosmetic compositions, pharmaceutical compositions and food compositions, and most preferably can be used in cosmetic compositions have. The plant is known as a plant which has no problem in using as a pharmaceutical composition or a food composition legally as a result of KFDA food raw material search (http://fse.foodnara.go.kr) and has an excellent effect on swelling or diabetes etc. It can be said that it can be used in a food or pharmaceutical composition showing a whitening effect in view of the above.

 The cosmetic composition of the present invention may contain other components than the above-mentioned plant extracts. According to an embodiment of the present invention, the cosmetic composition of the present invention is obtained from the group comprising glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Further comprising at least one auxiliary component selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, and at least one auxiliary ingredient selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin And most preferably at least one component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, But are not limited to, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

According to a preferred embodiment of the present invention, the composition of the present invention may be a pharmaceutical composition excellent in whitening effect.

When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers to be contained in the pharmaceutical composition of the present invention are those conventionally used in the formulation and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans in various routes such as oral or parenteral routes such as oral, rectal or intravenous, muscular, subcutaneous, intra-uterine, Can be administered by injection. Preferably, it is applied by transdermal administration during parenteral administration, more preferably by topical application by application.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . The dose of the pharmaceutical composition of the present invention can be administered in an amount of 0.1-100 mg / kg on an adult basis once or several times a day in the case of an oral formulation, and in the case of an external preparation, It is preferable to apply it once to 5 times a day in an amount of 3.0 ml and continue for 1 month or longer. However, the dosage is not intended to limit the scope of the present invention.

The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in any form suitable for pharmaceutical preparations including oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injectable solutions, , Dispersants, or stabilizers.

According to a preferred embodiment of the present invention, the composition of the present invention may be a food composition having an excellent whitening effect.

When the composition of the present invention is prepared with a food composition, it includes not only the active ingredient, but also ingredients normally added during the manufacture of the food, including, for example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavoring agents . Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, have.

As a result of analyzing cell viability with respect to the active ingredient of the composition of the present invention in the concrete examples of the present invention, it was found that the active ingredient of the composition of the present invention is a harmless substance as a natural substance. Therefore, since the active ingredient of the present invention has little toxicity and side effects, it can be safely used even for long-term use, and can be safely applied to cosmetic, pharmaceutical and food compositions as described above.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides a whitening cosmetic composition comprising a plant extract of Vitis flexuosa Thunb or Penthorum chinense PURSH as an active ingredient.

(b) The cosmetic composition of the present invention exhibits a remarkable whitening effect without skin irritation.

(c) The cosmetic composition of the present invention suppresses the tyrosinase enzyme, which is an enzyme important for the melanin production mechanism, and suppresses the amount of melanin production, thereby exhibiting a remarkable whitening effect.

(d) In the case of plant extracts as an active ingredient of the present invention, it can be used as a pharmaceutical composition and a food composition as well as the whitening cosmetic composition described above.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Example

Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.

Example  1: Preparation of water extract

First, 0.1 kg of Vitis flexuosa Thunb and Penthorum chinense PURSH were prepared as topical, top, flower, leaf, stem, root, fruit and seed samples, respectively. New muller and octopus leg specimens were placed in 5 times as much weight of purified water and heated at 105 ° C for 5 hours. When the extraction process is finished, it is filtered with Whatman paper No. 1, re-filtered through 0.45 ㎛ membrane filter (TF-450, 60173, PALL Life Sciences) Followed by concentration with a rotary vacuum evaporator followed by lyophilization. As a result, the weight of the water extract of the octopus legumes was 10.56 g, the weight of the water extract was 10.56 g, and the weight of the water extract of the octopus legumes was 9.26 g, the top part 9.97 g, the flower 8.47 g, the leaf 10.22 g, the stem 12.29 g, the root 9.28 g, 13.59 g, flower 12.12 g, leaf 9.00 g, stem 9.94 g, root 8.28 g, fruit 11.20 g, seed 10.08 g.

Example  2: Preparation of methanol extract

First, 0.1 kg of Vitis flexuosa Thunb and Penthorum chinense PURSH were prepared as tops, tops, flowers, leaves, stems, roots, fruits and seeds, respectively. Three times extraction solvent (methanol) was added to 0.1 kg of new mulberry and octopus leg samples and shaking extraction was performed at 25 캜 for 72 hours. The extract was filtered with filter paper and filtered through a 0.45 탆 membrane filter The mixture was concentrated by using a rotary vacuum evaporator and lyophilized to obtain 9.04 g of the methanol extract of the outgrowth of the myrtle, 8.30 g of the top part, 9.11 g of the flower, 10.12 g of the leaf, 9.53 g of stem, 8.40 g of root, 11.25 g of fruit, 9.00 g of seed, 9.15 g of methanol extract of octopus legume outpost, 14.52 g of ground portion, 12.05 g of flower, 9.94 g of leaf, 8.53 g of stem, 7.11 g of root, 10.02 g of seeds were obtained.

Example  3: Hexane  Preparation of extract

First, 0.1 kg of Vitis flexuosa Thunb and Penthorum chinense PURSH were prepared as tops, tops, flowers, leaves, stems, roots, fruits and seeds, respectively. The process of adding 50 times of extraction solvent (hexane) to 0.1 kg of new mulberry and octopus leg specimens and shaking extraction at 25 ° C for 5 days was performed once. The extract was filtered with filter paper and filtered through a 0.45 μm membrane filter , 60173, PALL Life Sciences), concentrated using a rotary vacuum evaporator, and lyophilized to give 11.00 g of a hexane extract of a bird's head outpost, 10.25 g of a top portion, 10.32 g of a flower, 9.88 g of a leaf, 9.27 g of root, 10.71 g of root, 9.53 g of fruit, 8.24 g of seed, 8.28 g of methanol extract of octopus legume, 10.11 g of root extract, 12.77 g of ground portion, 8.36 g of flower, 8.44 g of leaf, 9.76 g of stem, 8.55 g of root, 9.30 g was obtained.

Example  4 : Butylene glycol  Preparation of extract

First, 0.1 kg of Vitis flexuosa Thunb and Penthorum chinense PURSH were prepared as tops, tops, flowers, leaves, stems, roots, fruits and seeds, respectively. The extract solution was filtered through a filter paper and a membrane filter (TF-450, manufactured by Mitsui Chemicals, Inc., Japan) for 1 day. The extraction was carried out at 25 ° C for 5 days with shaking extraction by adding 10 times extraction solvent (butylene glycol) 60173, PALL Life Sciences), 0.724 kg (solid content: about 2.0%) of butylene glycol extract from the outpost of the bird head, 0.788 kg (solids content about 1.9%) on the ground part, 0.525 kg (Solids content about 2.2%), stem 0.792 kg (solids content 2.0%), root 0.705 kg (solid content about 4.5%), fruit 0.775 kg (solids content about 3.0%), seed 0.733 kg Butylene glycol extract 0.701 kg (solid content 2.7%), ground part 0.724 kg (solid part 2.4%), flower 0.537 kg (solid part about 4.0%), leaf 0.503 kg (solid part about 2.3%), stem 0.789 kg 0.755 kg (solid content: about 4.3%), 0.738 kg (solid content: about 2.0%) of seeds, and 0.710 kg (solid content: about 1.7%) of seeds.

Experimental Example  1: Cytotoxicity measurement

The cytotoxicity of the extract obtained by the method of the above Example was measured. In order to examine the cytotoxicity of the extracts according to the present invention, B16F1 (mouse melanoma cell) was added at a concentration of 5 × 10 ⁴ cells / ml to each well of a 96-well plate at 37 ° C. for 24 hours in a CO ₂ incubator Lt; / RTI > The medium was DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% bovine serum and 1% antibiotics. After culturing, the new mulberry and octopus legs were treated for each concentration, and cultured for 72 hours under the same culture conditions. After the incubation, the culture medium was removed and the culture solution containing 5 ㎎ / ㎖ of Neutral red reagent was added and cultured at 37 ° C for 2 hours. The culture was then removed, treated with 1% CaCl ₂ and formaldehyde, shaken at 400-500 rpm for 1 min, and then discarded. 1% Acetic acid and 50% Ethanol were added to each well and shaken for 10 minutes. Then, the absorbance was measured at 570 nm / 655 nm using a microplate reader. And Table 2.

Here, the control in the cytotoxicity test means a blank in which the above extract is not added.

Samples (占 퐂 / ml) part Control One 5 10 25 50 75 100 Birdhead
Example 1 Growth rate (%) outpost 100 94.25 95.14 93.78 98.02 93.67 89.45 86.31 Above ground 100 95.07 95.24 96.52 97.30 92.04 88.03 87.39 Flower 100 98.89 97.39 97.34 92.11 88.97 85.24 88.38 leaf 100 99.77 96.24 96.78 93.00 87.69 90.27 91.16 stem 100 96.79 97.01 96.67 95.54 91.08 88.34 86.24 Root 100 97.52 93.87 92.77 87.28 83.04 81.11 - Fruit 100 93.87 92.44 90.90 89.21 83.34 80.04 - strain 100 100.88 97.53 98.30 88.24 83.46 - - Birdhead
Example 2 Growth rate (%) outpost 100 96.86 96.66 93.27 92.22 85.85 - - Above ground 100 94.77 96.38 95.55 98.07 90.24 88.88 81.65 Flower 100 98.84 97.54 98.16 91.44 82.34 - - leaf 100 99.99 94.45 94.50 87.36 89.23 87.05 81.26 stem 100 96.58 97.56 92.34 85.50 - - - Root 100 99.72 89.09 84.55 81.14 - - - Fruit 100 95.66 85.10 82.36 80.33 - - - strain 100 97.55 93.34 95.86 92.44 93.34 90.04 89.27 Birdhead
Example 3 Growth rate (%) outpost 100 101.13 98.66 100.57 97.53 92.24 91.17 89.88 Above ground 100 96.77 98.89 99.64 94.38 92.00 89.08 85.35 Flower 100 95.55 96.67 98.43 92.04 86.34 81.11 - leaf 100 98.80 93.33 89.89 88.90 90.29 85.34 82.22 stem 100 98.84 91.43 92.46 90.04 88.88 82.30 - Root 100 92.31 91.24 90.00 84.28 83.33 - - Fruit 100 100.03 95.39 96.34 94.78 91.05 88.43 81.03 strain 100 95.55 93.54 89.66 90.24 91.33 85.32 85.00 Birdhead
Example 4 Growth rate (%) outpost 100 98.88 98.23 95.38 96.37 93.20 94.27 92.31 Above ground 100 96.99 97.04 95.55 94.94 95.01 92.05 90.33 Flower 100 98.84 96.36 95.51 92.22 93.93 91.05 92.22 leaf 100 100.32 99.87 98.54 94.56 96.60 95.00 95.12 stem 100 98.45 96.75 97.77 95.03 93.38 92.22 90.04 Root 100 97.04 95.55 94.43 91.36 89.64 88.42 85.47 Fruit 100 92.20 91.54 90.00 85.36 82.09 80.11 - strain 100 96.69 95.41 93.57 91.50 90.93 85.63 84.16

Samples (占 퐂 / ml) part Control One 5 10 25 50 75 100 Octopus leg
Example 1 Growth rate (%) outpost 100 99.01 97.25 98.14 92.70 90.23 85.50 - Above ground 100 101.88 97.86 94.47 92.08 91.13 88.89 83.26 Flower 100 96.69 92.44 92.73 94.00 87.50 81.12 - leaf 100 102.70 105.29 98.24 94.97 96.66 87.27 85.38 stem 100 95.55 95.26 88.71 82.33 79.77 - - Root 100 93.22 90.44 91.34 82.14 - - - Fruit 100 91.99 90.73 84.20 78.88 - - - strain 100 90.01 88.88 83.21 82.40 - - - Octopus leg
Example 2 Growth rate (%) outpost 100 95.54 96.96 90.13 83.74 - - - Above ground 100 92.23 88.72 84.05 78.93 - - - Flower 100 97.77 95.53 93.05 89.56 82.36 - - leaf 100 96.01 98.47 90.00 86.24 83.33 - - stem 100 95.48 94.25 89.35 83.65 77.82 - - Root 100 89.87 86.36 80.04 - - - - Fruit 100 94.07 84.43 78.81 - - - - strain 100 89.80 92.99 95.31 77.25 - - - Octopus leg
Example 3 Growth rate (%) outpost 100 93.55 87.41 80.01 - - - - Above ground 100 92.79 88.82 82.28 79.55 - - - Flower 100 91.42 83.64 78.77 - - - - leaf 100 93.33 91.45 87.02 81.72 - - - stem 100 91.69 84.23 80.00 - - - - Root 100 87.87 82.39 76.83 - - - - Fruit 100 89.98 90.34 81.36 - - - - strain 100 92.65 93.27 82.19 79.92 - - - Octopus leg
Example 4 Growth rate (%) outpost 100 97.79 94.23 90.34 82.33 - - - Above ground 100 99.00 92.07 88.39 83.11 - - - Flower 100 94.48 91.11 88.29 82.39 - - - leaf 100 92.33 93.58 94.03 83.24 77.16 - - stem 100 96.64 91.34 90.53 84.64 - - - Root 100 94.13 90.36 83.39 77.42 - - - Fruit 100 90.98 93.47 87.65 83.11 78.30 - - strain 100 91.72 89.08 93.55 80.68 - - -

-; Indicate cytotoxicity

As shown in Tables 1 and 2, when the cells were treated with 25 ㎍ / ㎖ of extracts from Octane extract and 10 ㎍ / ㎖ or less from Octopus legume extract, toxicity was not observed.

Experimental Example  2 : Tairosineiz  Measurement of inhibitory effect

The tyrosinase inhibitory effect of the extract obtained by the method of the above Example was measured. The tyrosinase inhibitory activity measurement was carried out by modifying a method such as Yagi, which measures DOPA chrome produced by the action of tyrosinase by a colorimetric method. That is, 60 μl of 1.5 mM L-tyrosine solution (in 0.1 M phosphate buffer (pH 6.5)) was added to 100 μl of a 0.1 M phosphate buffer (pH 6.5) in a 96-well plate, After adding 20 μl of the sample solution, 20 μl of mushroom tyrosinase (2000 unit / ml) was diluted 40 times with 0.1 M phosphate buffer, and the mixture was reacted at 37 ° C for 1 hour. Dopachrome was measured at 490 nm using a microplate counter. Arbutin (500 μg / ml) was used as a positive control for the comparison of test results. The results are shown in Table 3 below.

Samples (100 mu g / ml) Control Example 1 Example 2 Example 3 Example 4 Taisho Seinase low-performance Birdhead outpost 51% 16% 37% 25% 11% Above ground 51% 10% 32% 18% 19% Flower 51% 7% 14% 19% 8% leaf 51% 4% 21% 15% 8% stem 51% 8% 67% 29% 12% Root 51% 7% 9% 13% 4% Fruit 51% 22% 31% 23% 5% strain 51% 37% 45% 32% 23% Octopus leg outpost 51% 12% 54% 53% 20% Above ground 51% 19% 33% 24% 20% Flower 51% 22% 28% 8% 17% leaf 51% 18% 40% 16% 22% stem 51% 27% 13% 17% 23% Root 51% 15% 27% 20% 27% Fruit 51% 19% 29% 30% 25% strain 51% 30% 54% 22% 18%

Based on the results of Table 3, the tyrosinase inhibitory activity of each concentration was measured based on Example 2 showing the best tyrosinase inhibitory activity.

Samples (占 퐂 / ml) Control 100 75 50 25 Birdhead
Example 2 outpost 49% 33% 20% 15% 12% Above ground 49% 29% 24% 12% 8% Flower 49% 12% 24% 19% 2% leaf 49% 21% 23% 20% 7% stem 49% 62% 43% 28% 20% Root 49% 7% 4% 2% 13% Fruit 49% 36% 18% 5% One% strain 49% 31% 16% 18% One% Octopus leg
Example 2 outpost 49% 60% 39% 25% 19% Above ground 49% 37% 26% 22% 10% Flower 49% 33% 27% 12% 6% leaf 49% 29% 16% 10% 12% stem 49% 19% 9% 5% 4% Root 49% 23% 19% 14% 3% Fruit 49% 12% 11% 10% 6% strain 49% 61% 47% 32% 21%

As shown in Tables 3 and 4, in Example 2, it was found that the tyrosinase-like activity was significantly higher than that in Examples 1, 3 and 4, Also, it was found that the results were significant. In the case of arbutin (500 μg / ml), which is comparative in Table 4, the activity was 49%.

Experimental Example  3: Measurement of inhibition of melanin synthesis

The following experiment was conducted to determine the effect of suppressing the melanin biosynthesis in mouse-derived B16F1 melanoma. B16F1 (melanoma) cells were cultured in DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% of bovine serum and 1% of antibiotics, and the cell concentration was adjusted to 5 × 10 ⁴ cells / The cells were plated on a 60 mm diameter plate and cultured at 37 ° C and 5% CO ₂ for 24 hours. Subsequently, the culture medium was removed and 0.2 μM α-MSH (Melanocyte-stimulating hormone) was added to the wells except for the control. At this time, arbutin was used as a positive control at a concentration of 500 μg / ml. After culturing for 72 hours, the amount of melanin production in each plate was measured by removing the remaining culture medium and washing once with 1xPBS (phosphate buffered saline). Trypsin was treated in each well and incubated for 5 minutes in a CO ₂ incubator at 37 ° C to remove the cells. The cells were then treated with the culture medium and transferred to a 2 ml microtube. After centrifugation at 10,000 rpm for 10 minutes, the culture medium in the tube was removed, treated with 1 ml of PBS, and then washed again by centrifugation and removal. PBS was then removed and kept in a 70 ° C dryer for 1 hour with the tube lid open. The tubes taken out from the dryer were treated with 400 μl of 1 N NaOH (10% DMSO), respectively, and reacted in a 70 ° C. dryer for 10 minutes while the lid of the tube was closed. After the reaction, the mixture was shaken at 1,000 rpm for about 10 minutes, and 100 μl of each was transferred to a 96-well plate. The absorbance was measured at 490 nm / 655 nm using a microplate counter. The results of analyzing the inhibitory effect on melanin biosynthesis on the basis of the fresh mulberry stem, octopus legende and octopus leg seed extract of Example 2 showing the most excellent tyrosinase inhibitory effect are shown in Table 5 below.

sample Arbutin
(500 mu g / ml) Example 2
(25 [mu] g / ml) Example 2
(12.5 / / ml) Example 2
(6.25 [mu] g / ml) Stalk Inhibition rate
(%) 41% 64% 28% 7% Octopus leg outpost Inhibition rate
(%) 41% 59% 18% 2% Octopus leg seed Inhibition rate
(%) 41% 62% 27% 11%

As shown in Table 5, in Example 2, melanin biosynthesis in B16F1 melanoma cells was inhibited in a concentration-dependent manner, indicating that the whitening effect was excellent.

The following is an example of the composition of a cosmetic composition as a formulation having a whitening effect by containing a new mulberry and octopus leg extract in each cosmetic formulation. Here, the extract of Example 2, which has the best effect, was used.

Prescription example  1: Manufacture of softening longevity

Table 5 shows the prescription examples of the softened longevity in cosmetics containing at least one of the new mulberry stem, octopus legset and octopus legs seed extract of Example 2. The contents according to the components of Example 2 are based on the maximum concentrations which do not exhibit cytotoxicity as shown in Tables 1 and 2 above. In Table 6 below, the ingredients of Example 2 are not limited to the fowl.

 The specific manufacturing method is as follows. Sodium hyaruronate was dispersed in purified water in a propeller mixer at 3000 rpm to prepare a 1% solution. The raw material 1-8 was homogenized in a propeller mixer at 500 rpm and completely dissolved by heating at 75 DEG C, and then cooled to room temperature. The raw material 9-11 was completely dissolved in a separate dissolution tank and added to the above-mentioned water dissolution tank, followed by stirring and mixing. An extract of at least one of a bird's head stalk, an octopus leggedetal, and an octopus legged seed of Example 2 was put into the flask and stirred sufficiently to prepare a softened liquor containing the extract of Example 2.

ingredient Content (% by weight) New moth roots (Example 2) 0.0025 Polyoxyethylene hardened castor oil 0.5 Glycine 3.0 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaruronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 antiseptic 0.05 Purified water To 100

Prescription example  2: Manufacture of nutrition lotion

 Table 7 shows the prescription examples of the nutritional lotion among the cosmetics containing at least one of the new mulberry stem, octopus legset and octopus legs seed extract of Example 2. The contents according to the components of Example 2 are based on the maximum concentrations which do not exhibit cytotoxicity as shown in Tables 1 and 2 above. In Table 7 below, the ingredients of Example 2 are not limited to the fowl.

 The specific manufacturing method is as follows. The carbomer was prepared by dispersing the purified water in a propeller mixer at 4000 rpm in a 2% solution. The raw material 1-6 was added to the water dissolution tank and homogenized at 2000 rpm with stirring, and then heated to 75 ° C. Ingredients 7-13 were added to the oily melting tank and dissolved by heating to 75 ° C. The oil phase dissolved in an aqueous dissolution tank was charged and emulsified (3000 rpm / 5 minutes) and then cooled to room temperature. The extracts of at least one of the fresh mulberry stem, octopus legset and octopus leg seeds of Example 2 were put into the flask and mixed thoroughly with stirring to prepare a nutrition lotion containing the extract of Example 2.

ingredient Content (% by weight) New moth roots (Example 2) 0.0025 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoin 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 antiseptic 0.05 Purified water To 100

Prescription example  3: Preparation of essence

The prescription of the essence in the cosmetics containing at least one of the bird head stems, octopus legs outlets and octopus legs seed extract of Example 2 is shown in Table 8 below. The contents according to the components of Example 2 are based on the maximum concentrations which do not exhibit cytotoxicity as shown in Tables 1 and 2 above. In Table 8 below, the ingredients of Example 2 are not limited to the fowl.

The specific manufacturing method is as follows. Sodium hyaruronate and hydroxyethyl cellulose were dispersed in purified water in a propeller mixer at 2000 rpm to prepare a 1% solution. The carbomer was prepared by dispersing the purified water in a propeller mixer at 4000 rpm in a 2% solution. The raw material 1-12 was added to the water dissolution tank, homogenized at 2000 rpm, dispersed, and then heated to 75 캜. The warmed water phase was cooled to room temperature. The raw materials 13 to 15 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank and mixed by stirring. The extracts of at least one of the bird head stems, octopus legs outpost, and octopus leg seeds of Example 2 were put in and mixed thoroughly with stirring to prepare an essence containing the extract of Example 2.

ingredient Content (% by weight) New moth roots (Example 2) 0.0025 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaruronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl cellulose 0.2 ethanol 3.0 Antioxidant 0.3 Triethanolamine 0.1 Polyoxyethylene hardened castor oil 1.0 EDTA 0.1 antiseptic 0.05 Purified water To 100

Prescription example  4: Manufacture of nutritional cream

Table 9 shows the prescription examples of the nutritional cream among the cosmetics containing at least one of the new mulberry stem, octopus legset and octopus legs seed extract of Example 2. The contents according to the components of Example 2 are based on the maximum concentrations which do not exhibit cytotoxicity as shown in Tables 1 and 2 above. In Table 9 below, the ingredients of Example 2 are not limited to the fowl.

The specific manufacturing method is as follows. The raw material 1-8 was added to the water dissolution tank and homogenized at 2000 rpm, dispersed and then heated to 75 캜. The raw material 9-16 was added to the oily melting tank and dissolved by heating to 80 ° C. The oil phase dissolved in the water-dissolving tank was charged and emulsified (3000 rpm / 10 minutes) and then cooled to room temperature. The extracts of at least one of the bird's head stems, the octopus legs outpost and the octopus leg seeds of Example 2 were put in and mixed thoroughly with stirring to prepare a nutritive cream containing the extract of Example 2.

ingredient Content (% by weight) New moth roots (Example 2) 0.0025 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated lecithin 1.0 Octyldodecanol 3.0 Trioctanoin 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 antiseptic 0.05 Purified water To 100

Experimental Example  4: Whitening effect experiment

In order to confirm that the whitening effect shown in the previous experiment actually contributes to skin whitening of human beings, a human body test was performed on the cream containing the extract of at least one of the new horse hair stalk, octopus leg outpost, and octopus leg seed of Example 2 Respectively. The skin whitening test using the Roboskin analyzer equipment periodically evaluated the improvement of pigmentation and skin tone (brightness) while applying a sample or a product to the subject's face. The test was carried out in the same manner as the skin elasticity improvement test. Therefore, 10 subjects (5 control subjects, 5 test subjects) were used. The mean values were obtained by measuring 5 times in total for 1 week, 2 weeks, 4 weeks, and 6 weeks before use. All tests were conducted in a constant temperature and humidity chamber at 20-24 ° C and a humidity of 45-55% And then at least one hour in a humidity chamber.

Example 2 Before use 1 week 2 weeks 4 weeks 6 weeks Control Test section Control Test section Control Test section Control Test section Control Test section A bird outpost Pigmentation (small) 8.7 8.6 8.9 8.2 8.4 8.4 8.5 7.9 8.7 8.2 Pigmentation (large) 33.1 32.3 35.0 34.8 36.1 33.3 34.3 30.7 32.0 30.5 brightness 69.5 72.9 70.1 70.6 70.3 72.6 71.9 74.8 72.6 78.1 New ground top Pigmentation (small) 8.5 8.4 8.2 8.6 8.4 8.2 8.9 7.9 8.5 8.0 Pigmentation (large) 36.0 34.5 37.5 35.1 36.6 33.4 39.0 33.0 38.2 31.8 brightness 67.0 69.9 67.5 70.3 69.0 71.2 68.3 73.8 68.2 74.5 Birdhead
Flower Pigmentation (small) 8.0 8.0 7.8 7.7 8.1 7.9 8.6 7.5 8.3 7.5 Pigmentation (large) 33.8 31.4 33.2 32.5 31.8 31.3 28.8 29.7 31.8 28.9 brightness 62.3 67.0 63.9 65.1 64.4 69.5 64.8 69.8 65.3 72.3 New leaves Pigmentation (small) 8.2 8.6 8.3 8.5 8.4 8.2 8.7 7.8 8.6 8.1 Pigmentation (large) 30.1 31.9 31.7 30.5 30.2 29.7 29.4 28.0 28.8 27.3 brightness 63.8 68.2 64.7 70.0 65.2 71.3 62.1 73.5 62.9 73.4 Stalk Pigmentation (small) 8.9 8.7 9.1 8.7 9.0 8.4 8.8 8.0 8.7 7.3 Pigmentation (large) 31.0 32.2 30.8 32.0 30.1 29.7 30.8 27.8 29.9 23.2 brightness 68.2 71.1 68.0 71.2 68.1 73.8 72.5 77.7 70.4 81.0 New roots Pigmentation (small) 7.9 8.2 7.7 8.2 8.1 8.3 8.3 7.9 8.3 7.7 Pigmentation (large) 28.3 29.9 29.6 31.0 27.7 28.7 26.0 28.4 25.9 26.3 brightness 69.0 70.2 69.1 71.1 68.6 71.1 67.4 73.2 71.1 75.6 Birch nut Pigmentation (small) 8.2 8.4 8.5 8.4 8.2 7.8 7.9 7.9 7.8 8.2 Pigmentation (large) 29.2 30.5 27.6 28.8 28.1 26.4 27.1 25.2 25.9 23.4 brightness 71.2 73.6 72.3 74.6 72.8 75.9 73.1 76.6 73.3 77.7 Seedlings Pigmentation (small) 9.0 8.7 9.4 8.5 9.2 8.4 9.5 8.8 9.6 8.5 Pigmentation (large) 33.7 31.5 34.0 30.4 33.2 29.8 31.8 29.1 33.1 27.6 brightness 67.6 65.3 68.3 70.8 69.9 67.2 69.5 66.6 69.2 66.4

As a result of measuring the skin whitening effect on Prescription Example 4 of each part of the new head, the best efficacy of the new head of the present invention showed a tendency to decrease pigmentation (large and small) from the second week of administration and brighten the skin I know that it helps skin whitening.

Example 2 Before use 1 week 2 weeks 4 weeks 6 weeks Control Test section Control Test section Control Test section Control Test section Control Test section Octopus leg outpost Pigmentation (small) 10.1 9.8 10.0 9.5 9.6 9.0 9.7 8.1 9.3 7.8 Pigmentation (large) 32.5 32.8 32.8 32.0 31.1 29.4 30.4 27.3 31.0 24.9 brightness 65.5 68.0 65.4 69.2 68.3 71.7 63.1 75.1 64.5 77.0 Octopus legs above ground Pigmentation (small) 9.3 9.5 9.5 9.3 9.0 9.1 8.9 8.6 8.7 8.6 Pigmentation (large) 33.5 34.0 35.7 33.2 35.1 33.4 33.8 31.5 31.2 29.8 brightness 66.6 65.4 66.0 66.7 61.2 70.3 69.7 69.1 70.0 73.2 Octopus leg
Flower Pigmentation (small) 8.9 8.6 8.7 8.9 8.5 8.3 8.8 7.9 9.0 8.3 Pigmentation (large) 36.7 39.4 36.2 38.5 35.1 36.8 34.5 35.7 35.0 34.6 brightness 62.8 64.5 63.4 65.8 64.0 66.4 64.5 68.1 64.1 69.7 Octopus legs leaves Pigmentation (small) 8.7 8.5 8.9 8.2 9.0 8.3 8.6 8.1 8.7 7.9 Pigmentation (large) 35.1 37.0 34.7 36.9 36.4 35.5 36.5 31.2 35.7 30.8 brightness 65.0 68.1 64.2 67.4 65.2 68.2 66.1 69.8 65.7 70.3 Octopus legs stem Pigmentation (small) 8.5 8.9 8.6 8.8 8.2 8.6 7.9 8.1 7.8 7.8 Pigmentation (large) 32.8 35.4 33.8 35.9 32.2 33.3 32.8 31.2 32.0 30.3 brightness 63.7 65.5 64.5 67.0 65.0 68.3 67.1 69.7 66.6 71.1 Octopus leg root Pigmentation (small) 8.7 9.1 8.8 8.8 8.8 8.6 8.7 8.7 9.1 8.3 Pigmentation (large) 34.4 32.8 34.8 33.3 33.7 31.0 32.9 29.9 33.1 28.7 brightness 68.6 68.1 69.2 68.8 69.1 69.3 67.0 71.0 68.8 72.3 Octopus legume Pigmentation (small) 9.1 8.8 9.2 8.6 9.5 8.2 9.1 8.3 8.8 8.1 Pigmentation (large) 32.4 33.5 31.0 33.5 32.4 31.8 31.1 30.4 30.8 28.8 brightness 66.5 65.9 68.0 66.2 68.2 67.7 68.3 69.8 69.9 72.2 Octopus leg seed Pigmentation (small) 9.5 9.7 9.5 9.6 9.4 8.4 8.6 7.3 9.3 6.9 Pigmentation (large) 30.2 28.7 30.8 31.0 30.1 26.4 30.8 22.2 29.9 20.1 brightness 69.3 70.0 68.0 71.1 72.3 74.9 72.5 76.8 71.4 79.9

As a result of measuring skin whitening effect on Prescription Example 4 of octopus legs, pigmentation (large and small) of octopus leg shoot and octopus leg seed showed the best efficacy showed a tendency to decrease, and skin brightness was also brighter And it was found that it helps skin whitening.

As has been confirmed in the above experimental example, the extracts of new mulberry and octopus legs according to the present invention have a whitening effect. In particular, new mulberry stem, octopus legendus and octopus leg seed have excellent whitening effect. The cosmetic composition prepared by mixing at least one of the extracts of the present invention also has a whitening effect.

Accordingly, it is an object of the present invention to provide a new mulberry and octopus leg extract and a cosmetic composition containing at least one thereof, which inhibits the production of melanin in skin cells to help whitening.

Claims (6)

Vitis flexuosa Thunb plant extract as an active ingredient,
Such plants include plants, such as flowers, leaves, stems, fruits, seeds, roots and outposts
≪ / RTI >
delete The method according to claim 1,
Wherein the extraction solvent of the extract is selected from the group consisting of water, ethanol, methanol, methylene chloride, ethyl acetate, butanol, hexane, butylene glycol, chloroform and mixtures thereof.
The method according to claim 1,
Wherein the amount of the plant extract is 0.00002-30% by weight based on the total weight of the whitening cosmetic composition.
Vitis flexuosa Thunb plant extract as an active ingredient,
Wherein said plant is selected from the group comprising plants flowers, leaves, stems, fruits, seeds, roots and outposts.
Vitis flexuosa Thunb plant extract as an active ingredient,
Wherein the plant is selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and outposts of plants.