JPS62186784A - Water-based solid medium - Google Patents

Abstract

PURPOSE:To obtain the titled medium for the proliferation of living vegetable or animal cell or microorganism, by using xanthan gum in combination with locust bean gum as a colloid. CONSTITUTION:The objective medium contains xanthan gum and locust bean gum as a colloid. The weight ratio of the gum components id preferably 1:1. EFFECT:Since the medium is transparent, the behavior of the cell, etc., in the medium can be easily observed. The proliferation and growth of cultured cell, tissue, etc., are rapid in the medium.

Description

[Detailed Description of the Invention] [Industrial Application Field] This invention relates to the use of living active cell tissues of animals and plants;
This applies to media for artificially growing microorganisms such as yeast and bacteria.

[Conventional technology]

Agar is usually used as the material for solid media for culturing living active plants, animal cell tissues, microorganisms, etc., and other leg substances are rarely used.

Here, the solid medium is a name for a fluid fabric (including dimensions based on quality as a general rule), and is one of the nationally owned shares of the medium.
It refers to an aqueous system that uses soybean paste to form a gel.

[Problem that the invention seeks to solve]

A solid medium using agar is always cloudy, making it difficult to inspect or observe its interior from the outside with the naked eye or a microscope.

When attempting to obtain a callus or an adult plant by, for example, planting living active plant cells, observing the growth process of the callus, the state of rooting, and other behaviors of the cell mass or the adult plant is It is extremely difficult.

[Problem of invention]

Therefore, our challenge is to create a solid medium that is transparent and meets industrial expectations.

This invention is one solution to this problem, and is an industrially advantageous solution to this problem.

[Means to solve the problem]

This invention will be explained in detail below.

In place of the conventional agar, the colloids employed in this invention are xanthancum and locust bean gum, among the known ones. Neither xanthancum nor locust bean gum will gel when used alone, but when used together, they will gel and become a clear solid. In other words, the two are combined to form a colloid.

In the medium of this invention, the mixing ratio of both materials can be determined arbitrarily in order to form a gel, but in order to obtain the optimum solidity of the medium, Vζ
It is best to make both of them approximately equal in weight.

A mixture of xanthan gum and locust bean gum is used as an aqueous system. At that time, the total direct judgment of both is about 0.
Blend so that it is 5 to 1.0% (weight, same below),
Make it homogeneous. The thread is heated to form a sol, then cooled to form a gel, and allowed to cool to room temperature and solidify.

At this time, it is clear that tin, phosphoric acid, potassium-based substances, known cell growth hormones such as auxin and cytokinin, and trace components may be added to the medium in advance.

An additional benefit is the clear aqueous solid medium that is the object of this invention.

Here, this invention achieves its purpose.

[) Effects and effects of εming]

This product has the following characteristics.

■Since it is clear, the behavior of me @ etc. from the outside to the inside of the medium 1
The crane can easily see the light.

■Proliferation and growth of cultured cells and tissues is faster than on agar media. The reason for this is not clear at this time.

Below, the correctness of the above judgment will be explained by describing an experimental example.

Explanation ■Medium composition A, cucumber seeds, radish seeds... water only, mountain burdock, and tobacco callus/old...
- The same medium described below was used.

Ammonium nitrate 1650My
Potassium nitrate 1900〃
Potassium phosphate l '7Q
//Calcium chloride・2H20440η Magnesium sulfate・7H20370 with manganese sulfate-4FI20 22.
31/boric acid
6.27 Zinc sulfate/4H208,6□ Potassium iodide 0.88N
Sodium molybdate 2H200,25 sulfuric acid 1st
Copper-5H200,025// Cobalt chloride/6H200,025 Sodium EDTA 3
7.3 Ferrous sulfate, 7Hz0
27.8Il myo-inositol
1OOq thiamine hydrochloride
0.4 2.4-dichlorophenoxyacetic acid o, 22q sucrose
3 (Add each colloid in the table above to one or more ingredients, make 1 g with water, and adjust the I)H to 5.7. Place the homogeneous solid medium in an Erlenmeyer flask and add it to the aluminum bottle (aerated, water-filled). (difficult to evaporate), then sterilized (120°C for 15 minutes), cooled to room temperature, and tested.

■Culture conditions Temperature 25°C Lighting 3. OOO/L/Cultivation Cucumber and radish seeds 7 days Pokeweed and tobacco callus 35〃■ Planting of fresh Pokeweed and tobacco callus i0.3
r■Growth degree A: Seed germination of cucumbers and radish is indicated by stem length and root length (double). ) For callus of pokeweed and tobacco, it is expressed as the fresh weight of the harvested callus (2).

Claims (1)

[Claims] An aqueous solid medium for growing active cells of plants and animals, or microorganisms, characterized by using xanthan gum and locust bean gum as colloids in combination.