JPS61207470A - Production of concentrated paprika pigment - Google Patents

Abstract

PURPOSE:To obtain easily the titled pigment in a high yield, which has excellent developability and stability, is odorless, and has a high purity and a good quality, by treating paprika (or treated material) with lipase and subjecting the resulting pigment-contg. layer to a partition extraction treatment with a paraffinic hydrocarbon and a hydrous (KOH) alcohol. CONSTITUTION:Paprika and/or a treated material such as paprika orange or paprika juice are/is treated with lipase, while stirring in water, to effect hydrolysis. A paraffinic hydrocarbon such as n-hexane is added thereto, and the mixture is stirred and then left to stand, whereby it is separated into two layers. The lower layer is separated to obtain a paprika pigment-contg. hydrocarbon layer. A 60-95% aq. soln. of an alcohol or an aq. soln. contg. 1-5% KOH and 60-90% alcohol is added thereto to effect partition and extraction, whereby free fatty acids, tannin and malodorous materials are extracted in the hydrous alcohol layer. If desired, this operation is repeated to completely extract these material and the hydrocarbon is distilled off to obtain the titled pigment of good quality.

Description

[Detailed description of the invention] [Industrial field of application] /zo silica pigment is
It is a red pigment contained in the fruits of nuumL, ).

It is a red natural pigment and is widely used in various fields.

The present invention is a novel paprika pigment, which is industrially advantageous and easy to produce in high yield, and is a rich paprika pigment with excellent coloring properties, stable and odorless. The present invention provides a method.

The silica pigment obtained in this way is used as a high-quality, concentrated natural coloring agent in foods, beverages, and luxury foods.

It is widely and importantly used in industrial technology fields such as pharmaceuticals and cosmetics.

[Conventional technology]

Traditionally, paprika pigments have been produced using paprika fruit and its dried powder, etc., using soybean oil, rapeseed oil, coconut oil, cottonseed oil,
5. Extract with animal and vegetable oils such as corn oil and fish oil. J
Oil-dissolution methods in which silica pigments are eluted into these oils, solvent methods in which they are extracted with organic solvents such as alcohols, ketones, and halogenated hydrocarbons, and other methods are known.

However, with this method, it is difficult to obtain a concentrated dye solution, and the obtained dye is accompanied by an unusual odor peculiar to No.

As a method for improving these drawbacks, a method has been proposed in which heat treatment is performed with an alkaline aqueous solution and the dye is treated with an organic solvent (4! Publication No. 52-1742).

However, in the above-mentioned proposed method, since the alkali-treated product becomes a solid substance, the dye extraction efficiency is poor and the operation is extremely complicated, making it unsuitable for industrial production.

Furthermore, the dye ester itself is hydrolyzed by alkali treatment, resulting in a change in one color tone or fading, and even if you try to prepare a rich dye, the dye will precipitate, making it difficult to achieve the desired purpose. I can't.

[Purpose of the invention]

As described above, it has not been possible to advantageously and satisfactorily prepare concentrated Nozopurika pigments, or even ordinary Nozopurika pigments, using conventional techniques. It is.

The present invention has been made to solve these drawbacks, and is to produce a paprika pigment that has excellent color development, is rich, stable, and exhibits no odor, and is industrially advantageous and easy to produce in good yield. The purpose is to manufacture.

[Structure of the invention]

The present invention has been made to achieve the above object, and based on the viewpoint that the desired object cannot be achieved by relying only on physical and chemical methods, the present invention is based on the conventional idea. This led to a fundamental shift and focus on biological methods.

As a result of examining biological methods from various angles,
They found that a method using enzymes is effective, and based on this new knowledge, they conducted extensive screening for enzymes and found that lipase treatment is extremely effective.

However, it is not possible to obtain the desired high-grade, concentrated noseplica pigment only by the Lino-de-ze treatment.

Further post-processing is required. Therefore, as a post-treatment, they came up with the idea of organically combining a partition extraction process using an organic solvent with a lipase treatment, and particularly completed the present invention.

That is, the present invention subjects paprika and/or its processed product to lipase treatment to selectively saponify only the fat component, which is a malodorous component, without changing the Noblica pigment ester, and then to process the by-produced fatty acids, The present invention relates to a method for producing a concentrated paprika pigment, in which the important point is to wash and remove odor components using an organic solvent.

As the raw material for treatment, the turmeric itself and/or its treated product are used. Paprika processed products include paprika oleoresin, /J! Delica (crude) extract, ground paprika, paprika juice, concentrated liquid thereof, freeze-dried powder thereof, dried powder of paprika, etc. can be freely used.

The lipase-treated products of these treated raw materials do not become soap-like solids, and only the fat content, which is the main cause of odor, is selectively saponified. The lipase-treated product is then dissolved in an organic solvent. As an organic solvent, hantan,
Hexane, heptane.

Paraffin hydrocarbons such as octane, nonane, decane,
Aromatic hydrocarbons such as benzene and xylene.

Alcohols, ketones, halogenated hydrocarbons, and the like are widely used.

After dissolving in these organic solvents, washing with a hydrous caustic alkaline-alcohol solution and a hydrous alcohol solution,
If fatty acids and malodorous components are removed.

Excellent color development. A stable, odorless, high-quality, concentrated /ζ silica dye can be industrially advantageously produced in a good yield. The order of treatment with the hydrated caustic alkaline-alcohol solution and the hydrated alcohol solution may be arbitrary, and if necessary, a sufficient cleaning effect can be obtained by treatment with one of the solutions. If necessary, these washing treatments may be repeated with or without changing the concentration of caustic alkali and/or alcohol. As the caustic alkali, caustic soda and caustic potash are used, and as the alcohol, methanol, ethanol, propatool, butanol, and other alcohols can be freely used.

As the lipase, 9,-J-ases of various origins are widely used, and commercially available enzymes of microbial origin, such as 97J-se MY, Lipase AP, and Lipase (Cykun), can be advantageously used. When performing lipase treatment, lipase 0.1 to 5. Preferably 0.5 to 1 weight to 50% water
~500. Preferably, after adding and dissolving 150 to 250 weight, 50 to 500.
Preferably add 150 to 250 weight and heat at 15 to 40'C.
1. The saponification reaction may be carried out by stirring preferably at 20 to 30°C for 1 to 20 hours, preferably 5 to 10 hours. Note that an organic solvent such as n-hexane may be added in advance during the saponification reaction.

After completion of curing, an organic solvent such as n-hexane is added and stirred, the lower layer is separated, and the paprika pigment-containing organic solvent layer is washed with a hydrous caustic potassium alcohol solution. The concentration of the caustic potash alcohol solution is 1 to 10ts, respectively.
, it is good to set it to about 50 to 90 inches, and a particularly suitable value is 3
-5 inches, 70-80 inches. Then% 50-95%
, preferably with an 80% alcohol solution to completely remove fatty acids and odor components. The order of the cleaning treatment may be the reverse of this order, or either one or the other.

You may also repeat the process. After the washing process is completed, the organic solvent such as n-hexane is distilled off to obtain a high quality, concentrated Nogpurika dye.

〔Effect of the invention〕

The paprika pigment obtained by the method of the present invention has the following remarkable effects as a natural coloring agent.

■ Excellent color development and one hue is stable over time.

■ Manufacturing operations are safe and easy.

■The color tone is almost the same as that of oleoresin.

■ Phenolic substances such as tannins are completely removed.

■ It is odorless and has excellent quality.

■ Dye does not precipitate even at high concentrations.

The paprika pigment of the present invention can be used as a natural coloring agent in a wide range of fields such as food and beverages, luxury foods, pharmaceuticals, cosmetics, and others.

The attempt to use lipase in the production of paprika pigment is a new and previously unknown technology, and 9a! ! Only by organically combining the -ze treatment and the organic solvent treatment can very advantageous high-grade concentrated paprika pigments be obtained.

Example 1 After dissolving 1 g of lipase MY in 200.9 g of water, 200 g of No.
Add g. Furthermore, 200 I of n-hexane was added, and the mixture was stirred and reacted at room temperature for 4 hours. After completion of the reaction, 60 n-hexane
Add 0I, stir and dissolve, then leave to stand and separate the lower layer.

The Nono silica-containing n-hexane layer (upper layer) is washed with 600 g of 90% ethanol, and further washed with 600 J/of a 15N-75 diethanol aqueous solution.

After washing three times with 300 g of leprosy 80-ethanol solution,
N-hexane was collected to obtain 48J of concentrated paprika.

The color value was 355.100, and the paprika color was odorless and of excellent quality.

Example 2 Dissolve lipase MY500p in 100 #IC of water.

Paprika Oleoresin (Color Value 97.280
) 10 QIcg and 100 g of n-hexane were added, and a saponification reaction was carried out at room temperature for 6 hours.

After the saponification reaction, 500 g of n-hexane was added, and after separation, the paprika-containing n-hexane layer (upper layer) was washed with 280 ethanol and 15 # of water, and then 200 g of ethanol was added.
After washing with 170 kg of ethanol and 40 IcII of water, the sample was washed twice with 170 kg of ethanol and 40 IcII of water.

Thereafter, hexane was recovered to obtain Paprika 23#.

The color value was 822.620, and it was an odorless and excellent pigment.

Agent: Patent attorney, Todo 83, R-man Procedural amendment November 15, 1985

Claims (1)

[Claims] 1. A method for producing a high-quality concentrated paprika pigment, which comprises treating paprika or a processed product thereof with lipase, and then subjecting it to partition extraction treatment using an organic solvent.