JPS5858439A - Counting apparatus of thrombocyte - Google Patents

Abstract

PURPOSE:To count a thrombocyte easily and surely, by controlling the display extent of information of grain distribution of hemocytesstored in an RAM by a cursor moving by an input operation. CONSTITUTION:A detection signal from a hemocyte detection apparatus 1 is processed by a comparator 2 having continuous many standard values set and a signal generating circuit 3, and a hemocyte grain signal is issued and then, a hemocyte cumulative distribution curve or hemocyte distribution curve, a grain distribution curve indicating a percentage and a calculation result, etc., are written in each RAM 6-8 of a read and write memory 12 through a control arithmetic device 4 corresponding to a program of an RAM 5. And, the positions of two pairs cursors are controlled by operating an input device 9 and the distribution curve of the RAM 6 or 7 in the range of setting by these cursors is displayed on a CRT 10 together with these cursors. Accordingly, the number of the thrombocytes having a small grain size is counted easily and surely by observing the display of the CRT 10 and selecting said display through the apparatus 9.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention eliminates the influence of red blood cell errors or abnormalities in the size of platelets themselves on platelet counts.

The present invention relates to a platelet counting device that provides a more accurate platelet count.

There are various methods for selecting and counting only platelets from among blood cells such as red blood cells, white blood cells, and platelets in the blood.

In the conventional method, platelets were pretreated, a sediment layer containing many platelets was taken out, counted and measured, and converted to the concentration before pretreatment. In this method, platelets are already separated from other particles such as red blood cells in the pretreatment step, so measurement can be performed using a simple blood cell counter, but the pretreatment required requires time and effort. and
Furthermore, there was a drawback that the platelet count could not be obtained unless conversion was performed.

In recent years, automation of analyzers has progressed, and it has become possible to perform classification, counting, and measurement simply by aspirating blood.

Taking advantage of the fact that platelets are smaller than red blood cells and other blood cell particles, it is proportional to the size of blood cell particles.1. A particle signal exceeding one threshold voltage is generated by a blood cell signal larger than a platelet by setting up an open circuit with a predetermined threshold voltage.
Particle signals smaller than the threshold voltage are determined to be platelets, and selective counting is performed.

Furthermore, another threshold voltage is provided to distinguish between a noise signal and a platelet signal, and generally a signal between the two threshold voltages is considered to be a platelet signal. This method allows signals between preset threshold voltages to pass through.

If some fluctuation occurs in the blood cell detection device, the sensitivity changes, or there is a problem, red blood cells, which are large particles, may become platelets Fct-t.
<There is a drawback that an accurate platelet count cannot be obtained with wrapped specimens. In particular, when the MCV (mean particle volume), which is a parameter of red blood cells, is 70 μm or less, the platelets themselves may be microcytic, and the overall size tends to be smaller, so the platelet count value is of course However, this technique has the drawback of causing a counting error in the number of red blood cells, which has a clinically significant impact.

The present invention has been made in view of the above-mentioned points, and is an attempt to solve the above-mentioned drawbacks and to provide a device that is easy to operate and can accurately count the number of platelets.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The configuration of the present invention will be explained below based on embodiments shown in the drawings. FIG. 1 is a systematic explanatory diagram showing one embodiment of the platelet counting device of the present invention. The platelet counting device of the present invention includes a blood cell detection device 1 that detects blood cells based on an electrical difference or an optical difference between blood cells and a blood cell suspension, and generates a signal proportional to the size of the blood cells; A comparison circuit 2 having a plurality of successive threshold values, for example, 50 or 100, for the blood cell signal from the device 1, and a signal generation circuit that generates a signal representing the size of the blood cells based on the output signal of this comparison circuit 2. 3 - an arithmetic/control circuit 4 connected to this signal generating circuit 6; a successive memory 5 connected to this arithmetic/control circuit 4 in which arithmetic operations and control sequences are stored; and a memory 5 connected to said arithmetic/control circuit 4. Read/write memo 16.
7-8. Input device 91 Display device 10 and Recording device 11
It includes. The continuous write memory is the first memory 6.
゜It consists of a second memory 7 and a third memory 8, the first memory 6 stores the cumulative particle size distribution or the number of normal particle size distributions, the second memory 7 stores the particle size distribution to be displayed, and the third memory The memory 8 stores various calculation results or distribution curves obtained from the calculation results. Although the successive write memories 1J6.7.8 are shown separately provided for ease of understanding, it is also possible to divide the successive write memories 12 into three areas. Furthermore, the input device 9 is used to move two H-cursors 13 and 14 on the display of the display device 10,
．． Four push-button switches for displaying 14 positions 15.16. An IL 18 is provided, and is configured to input conditions for various calculations as the cart V moves.

When the blood cell signal from the blood cell detection device 1 is sent to the comparator circuit 2 in the platelet counting device configured as described above, the peak value of the signal or the comparator up to the peak value is turned on, and the circuit in are connected in parallel with as many output terminals as there are comparators. In the signal generation circuit 3, it is possible to connect an appropriate frequency dividing circuit in parallel with the number of terminals to match the calculation speed, or to prohibit the input of the blood cell signal during calculation for one pulse. The signal is used as a comparison signal for blood cell pulse intervals, and each terminal is scanned and the peak value of blood cells or the ON line up to the peak value is calculated by the arithmetic/control circuit 4 according to the program order of the read-only memory 5. 1 memory B sequentially memorize it. At this time, the book in which the peak value of blood cells is stored is a normal particle size distribution curve, and the book in which all the lines that are turned on up to the peak value are stored for each blood cell is a cumulative particle size distribution curve. Each address of the first memory 6 corresponds to a blood cell size, that is, a threshold value, and each address stores the number of blood cells. When the measurement of a predetermined volume of blood cell suspension fluid or a predetermined time is completed.

The first memory 6 according to the operation order of the successive memory 5
Each address is called in sequence, and (1) total number of blood cells - (2
) If the particle size distribution is stored in the form of cumulative particle size distribution, the difference in blood cell counts between adjacent addresses is 1. If the particle size distribution is normal, read the blood cell count at that address and divide it by the total number of blood cells in (1). Step-(3) The operation of storing the calculation results in the corresponding addresses of the second memory 7 is performed, and the particle size distribution curve as shown in the graph is obtained. There are four press L7 button switches as described above, and as shown in FIG. Two cursors 13 and 14 are displayed along with the platelet particle size distribution curve. The count value is displayed using these two cursors 15. ．． Blood cell count between 14 and 14. Namely.

For general measurements, use this two-tree cursor 16.1.
4 is fixed, as shown in Figure 3 - for example 2
．． 4μ” + 80.4μ3 1 Normal platelets are distributed within this range,
For example, in blood containing red blood cells with an MCV of 70 μ8, a particle size distribution curve biased toward the smaller side as shown in FIG. 8 is obtained. Therefore, a value of 289,000 pieces/wm is obtained.On the other hand, as shown in FIG. Push button switch 15-16
- Modify the threshold voltage with ICl3. When the push button switch 15 on the left of LOWBR is pressed, the left cursor 16 shown in FIG. 3 moves to the left. At this time, the position of the cursor 16 is shown. The numbers constantly change as the carts move, and the display APLT (
Analyzing PL, T) also changes. That is, each time the cursor 16 is moved by one address, the platelet count at that address is read out, added to the +pr and 'r values, and displayed as the -APLT value, which is repeated each time the address advances. Right cursor 14 UPPPEH left push button switch 1
If you move it to the left by pressing push button 7,
This time, the number at each memory address is read and subtracted.

As described above, as shown in FIG. 4, by setting Kurtz ν to a position considered to be optimal, a more accurate value of the platelet count can be obtained. Note that for normal blood, two cursors 13. as shown in FIG.
A particle size distribution curve that was approximately within the range of 14 was obtained, and there was no need to move the cursor.

As explained above, in the apparatus of the present invention.

The optimum threshold value can be set or corrected by directly looking at the particle size distribution curve displayed on the monitor cathode ray tube and by moving the curve simultaneously.

Furthermore, the corrected platelet count at that time can be read directly, and measurements can be taken under the same conditions for each sample until the cursor is moved. ).

Furthermore, unlike inputting conditions using numerical values, the system is self-explanatory and easy to set.

[Brief explanation of the drawing]

FIG. 1 is a systematic explanatory diagram showing one embodiment of the platelet counting device of the present invention, FIG. 2 is an explanatory diagram of push button switches provided on the input device, and FIG. 3 and FIG. 4 are operations on the display device. An explanatory diagram showing an example of % Figure 5 is a particle size distribution curve for normal blood. DESCRIPTION OF SYMBOLS 1... Blood cell detection device, 2... Comparison circuit, 6... Signal generation circuit, 4... Arithmetic/control circuit, 5... Memory for continuous output, 6... First memory, 7.・Second memory,
8...Third memory, 9...Input device, 10...Display device, 11...Recording device, 12...Continuous writing memory, 13.14...Cursor, 15- 16- 17
- 18...Push button switch

Claims (1)

[Scope of Claims] 1. A blood cell detection device that detects blood cells based on electrical or optical differences between blood cells and a blood cell suspension and generates a signal proportional to the size of the blood cells. A comparison circuit having a plurality of continuous threshold values for the blood cell signal from this blood cell detection device, a signal generation circuit that generates a signal representing the size of the blood cells from the output signal of this comparison circuit, and this signal generation circuit. an arithmetic/control circuit connected to the arithmetic/control circuit; a continuous write memory connected to the arithmetic/control circuit in which arithmetic operations and control sequences are stored; and a continuous write memory connected to the arithmetic/control circuit. The input device 1 includes a display device and a recording device, the successive write memory has a first memory, a second memory and a third memory, and the first memory has a cumulative particle size distribution or a normal particle size distribution number distribution. 3. A second memory stores the particle size distribution to be displayed, a third memory stores various calculation results or distribution curves obtained from the calculation results, and the input device stores information on the display of the display device. Four push-button switches are provided to move the two-tree cursors and display the positions of these cursors, and one to obtain the particle size distribution curve corresponding to platelets on the display of the first display device. A platelet counting device, characterized in that by moving these displayed cursors, the number of spheres at each address in the memory is read out, and a corrected number of platelets is obtained by performing addition and subtraction.