JPS56118672A - Measuring method of antigen or antibody - Google Patents
Measuring method of antigen or antibodyInfo
- Publication number
- JPS56118672A JPS56118672A JP2206380A JP2206380A JPS56118672A JP S56118672 A JPS56118672 A JP S56118672A JP 2206380 A JP2206380 A JP 2206380A JP 2206380 A JP2206380 A JP 2206380A JP S56118672 A JPS56118672 A JP S56118672A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- labeling material
- precipitate
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To measure the very small amount of antigen and antiboty with excellent sensitivity, accuracy, and reproducibility, by bonding a labeling material to the antigen or antibody, reacting the antibody with the labeling material with a bonded body of the antigen, the antibody, and the labeling material, and measuring the activity of the labeling material in the precipitate. CONSTITUTION:To either of the antigen or antibody, enzyme, e.g., beta-D-galactosidase and the like is labeled. The antibody or antigen is reacted thereto, and the bonded body of the antigen, the antibody, and the label is precipitated. In this case, if the amount of the bonded body is too small to be taken out, said enzyme which is inactivated is used as a carrier. In order to remove the co-precipitate such as interfering protein and the like, solution and reprecipitation are repeated as required. Thereafter, the antibody F(ab<1>)2 of the labeling material is added, and the precipitate is obtained. Or the material, obtained by fixing F(ab<1>)2, is bonded to the carriers of glass beads and the like. By measuring the activity of the enzyme in the resultant composite material of the antigen, the antibody, the labeling material, and the labeling material antibody, the measurement whose sensitivity is 100 times that in the conventional method can be achieved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2206380A JPS56118672A (en) | 1980-02-23 | 1980-02-23 | Measuring method of antigen or antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2206380A JPS56118672A (en) | 1980-02-23 | 1980-02-23 | Measuring method of antigen or antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS56118672A true JPS56118672A (en) | 1981-09-17 |
Family
ID=12072437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2206380A Pending JPS56118672A (en) | 1980-02-23 | 1980-02-23 | Measuring method of antigen or antibody |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56118672A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0264459A (en) * | 1988-07-05 | 1990-03-05 | Boehringer Mannheim Gmbh | Method and reagent for measuring substance, which can be bonded |
| JPH02107966A (en) * | 1988-08-29 | 1990-04-19 | Boehringer Mannheim Gmbh | Method of measuring specifically bonding substance and measuring reagent |
| JPH02216055A (en) * | 1988-12-23 | 1990-08-28 | Boehringer Mannheim Gmbh | Detection of compound containing carbohydrate |
-
1980
- 1980-02-23 JP JP2206380A patent/JPS56118672A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0264459A (en) * | 1988-07-05 | 1990-03-05 | Boehringer Mannheim Gmbh | Method and reagent for measuring substance, which can be bonded |
| JPH02107966A (en) * | 1988-08-29 | 1990-04-19 | Boehringer Mannheim Gmbh | Method of measuring specifically bonding substance and measuring reagent |
| JPH02216055A (en) * | 1988-12-23 | 1990-08-28 | Boehringer Mannheim Gmbh | Detection of compound containing carbohydrate |
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