JPH08119869A - Active oxygen suppressor - Google Patents

Abstract

PURPOSE: To obtain an active oxygen suppressor containing an extract of leaf of a plant selected from peppermint, oregano, marjoram, thyme, melissa, rosemary and basil. CONSTITUTION: This active oxygen suppressor contains a solvent extract of leaf of at least one kind of plant selected from a group of peppermint, oregano, marjoram, thyme, melissa, rosemary and basil. For example, leaf of rosemary is extracted with water or a hydrophilic organic solvent (e.g. ethanol) and lyophilized to provide powder. The powdery substance can be prepared into lotion, cream, emulsion, pack, etc., by mixing it with other cosmetic raw materials, etc. The cosmetic containing the substance has large effect for inhibiting production of active oxygen and a cosmetic containing the suppressor has good chapped skin-preventing effect and retaining effect of gloss and stretch of skin and the substance is a natural product and has been used for many years as a spice or condiment and has good safety to skin.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plant-derived active oxygen inhibitor applied as an active oxygen inhibitor to cosmetics, pharmaceuticals and foods.

[0002]

BACKGROUND OF THE INVENTION Peppermint, oregano, marjoram,
Thyme, melissa, rosemary and basil leaves are widely used as spices for food.

[0003]

It is an object of the present invention that it is known that it is a natural product and safe for the human body, and it has a strong active oxygen inhibitory action, and if possible, exerts an effect other than the active oxygen inhibitory action. It is to provide such an active oxygen inhibitor.

[0004]

[Means for Solving the Problems] In order to solve the above problems, the present inventors screened and investigated plants that have been used for food for many years and confirmed to be safe for the human body, and used as cosmetics. Considered something of value. As a result, they have found that peppermint, oregano, marjoram, thyme, melissa, rosemary, and basil leaves are very effective as cosmetic raw materials or quasi drugs.

That is, the present invention comprises peppermint, oregano, marjoram, thyme, melissa, rosemary,
It is an active oxygen inhibitor containing at least one plant leaf solvent extract selected from the group consisting of basil.

[0006] Peppermint, oregano, marjoram, thyme, melissa, rosemary, basil leaves can be used by extraction with water or a hydrophilic organic solvent such as ethanol, methanol or acetone. However, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable since it is extraction of cosmetic raw materials. In some cases, a polyhydric alcohol such as glycerin, 1,3 butylene glycol, propylene glycol or the like or a mixed liquid of polyhydric alcohol and water can be used for extraction.
Further, freeze-drying and using it as powder is also effective depending on the method of use.

[0007] This substance is used as other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various drugs. It can be added to prepare cosmetics in various dosage forms. For example, a lotion, a cream, a milky lotion, a pack, or the like may be used depending on the purpose.

There is oxygen in the air, and without it, no organisms (except anaerobic ones) can exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids and produces peroxides. It also oxidizes and damages the phospholipids of biological membranes in the body. Furthermore, it is said that the generated peroxide and active oxygen damage DNA and accelerate aging. This active oxygen also influences the mechanism of making melanin from tyrosine and is also involved in the blackening of the skin. Suppressing this active oxygen is an important factor for the skin, in other words, an important factor required for cosmetics.

[0009]

EXAMPLE An example of an actual usage will be described below, but the present invention is not limited to this example. Used in the present invention, peppermint, oregano,
An example of the production of leaf extract of marjoram, thyme, melissa, rosemary and basil is shown below.

(Production Example 1) Leaf of peppermint (dry product)
Ethyl acetate (300 ml) was added to 10 g and the mixture was allowed to stand for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 2) Leaf of peppermint (dry product)
300 g of ethanol was added to 10 g of the above and the mixture was allowed to stand for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 3) Leaf of peppermint (dried product)
300 g of a 50% aqueous ethanol solution was added to 10 g and the mixture was left for 5 days with occasional stirring. It was filtered, evaporated and freeze-dried.

(Production Example 4) Leaf of peppermint (dry product)
300 g of 50% methanol aqueous solution was added to 10 g of the above, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 5) 1 oregano leaf (dry product)
300 ml of ethanol was added to 0 g, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 6) 1 leaf of oregano leaf (dried product)
300 ml of 50% ethanol aqueous solution was added to 0 g, and the mixture was left for 5 days with occasional stirring. It was filtered, evaporated and freeze-dried.

(Production Example 7) 1 oregano leaf (dried product)
To 0 g, 300 ml of 50% aqueous methanol solution was added, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 8) 300 g of ethyl acetate was added to 10 g of marjoram leaves (dried product), and the mixture was stirred occasionally to give 5
Left for days. This was filtered, evaporated and freeze-dried.

(Production Example 9) To 10 g of marjoram leaves (dried product) was added 300 ml of a 50% aqueous ethanol solution, and the mixture was left for 5 days with occasional stirring. It was filtered, evaporated and freeze-dried.

(Production Example 10) 1 leaf of thyme (dried product)
300 ml of ethyl acetate was added to 0 g, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 11) 1 thyme leaf (dried product)
300 ml of ethanol was added to 0 g, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 12) 1 leaf of thyme (dried product)
To 0 g, 300 ml of 50% aqueous methanol solution was added, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 13) To 10 g of Melissa leaf (dried product) was added 300 ml of ethanol, and the mixture was stirred occasionally to give 5
Left for days. This was filtered, evaporated and freeze-dried.

(Production Example 14) To 10 g of Melissa leaf (dry product) was added 300 ml of 50% ethanol aqueous solution, and the mixture was left for 5 days with occasional stirring. It was filtered, evaporated and freeze-dried.

(Production Example 15) To 10 g of Melissa leaves (dry product) was added 300 ml of 50% methanol aqueous solution, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 16) To 10 g of Melissa leaf (dry product), 300 ml of purified water was added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

Production Example 17 To 10 g of rosemary leaves (dried product) was added 300 ml of ethyl acetate, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

Production Example 18 To 10 g of rosemary leaves (dry product) was added 300 ml of 50% ethanol aqueous solution, and the mixture was left for 5 days with occasional stirring. It was filtered, evaporated and freeze-dried.

(Production Example 19) To 10 g of rosemary leaves (dried product) was added 300 ml of 50% methanol aqueous solution, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 20) To 10 g of rosemary leaf (dry product), 300 ml of purified water was added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Production Example 21) 1 basil leaf (dry product)
300 ml of ethanol was added to 0 g, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Example-1) Lotion Olive oil 0.5 Production Example 1 Extract 0.5 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

(Example-2) Cream A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Production Example 2 Extract 1.0 B Purified water 47.9 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 Glycerin 5 0.0 1.0% aqueous solution of sodium hyaluronate 5.0 Methyl paraoxybenzoate 0.1 Weigh A and B respectively, warm to 70 ° C, slowly add A to B with stirring, and then slowly stir. While doing 30
Cooled to ° C.

Example 3 is a lotion prepared by replacing the extract of Production Example 1 of Example 1 with the extract of Production Example 3.

Example 4 is a cream prepared by changing the extract of Production Example 2 of Example-2 into the extract of Production Example 4.

Example-5 is a lotion prepared by changing the extract of Production Example-1 of Example-1 into the extract of Production Example-5.

Example 6 is a cream prepared by replacing the extract of Production Example 2 of Example-2 with the extract of Production Example 6.

Example-7 is a lotion prepared by changing the extract of Production Example 1 of Example-1 into the extract of Production Example 7.

Example-8 is a cream prepared by replacing the extract of Production Example 2 of Example-2 with the extract of Production Example 8.

Example-9 is a lotion prepared by changing the extract of Production Example 1 of Example-1 into the extract of Production Example 9.

Example-10 is a cream prepared by replacing the extract of Example-2 of Example-2 with the extract of Example-10.

Example-11 is a lotion prepared by changing the extract of Production Example 1 of Example-1 into the extract of Production Example 11.

Example-12 is a cream prepared by replacing the extract of Production Example 2 of Example-2 with the extract of Production Example 12.

Example-13 is a lotion prepared by changing the extract of Production Example 1 of Example-1 into the extract of Production Example 13.

Example-14 is a cream prepared by replacing the extract of Production Example 2 of Example-2 with the extract of Production Example 14.

Example-15 is a lotion prepared by changing the extract of Production Example-1 of Example-1 into the extract of Production Example-15.

Example-16 is a cream prepared by replacing the extract of Production Example 2 of Example-2 with the extract of Production Example 16.

Example-17 is a lotion prepared by changing the extract of Production Example 1 of Example-1 into the extract of Production Example 17.

Example-18 is a cream prepared by changing the extract of Production Example 2 of Example-2 into the extract of Production Example 18.

Example-19 is a lotion prepared by changing the extract of Production Example 1 of Example-1 into the extract of Production Example 19.

Example-20 is a cream prepared by replacing the extract of Production Example 2 of Example-2 with the extract of Production Example 20.

Example 21 is a lotion prepared by replacing the extract of Production Example 1 of Example-1 with the extract of Production Example 21.

(Effect of Active Oxygen Suppression Test) There are various methods for measuring the effect of suppressing active oxygen, but this time an experiment was carried out using SOD Test Wako of Wako Pure Chemical. Use the coloring reagent 1.
0 ml, 0.1 ml of 0.1% solution of the sample was taken to a constant temperature at 37 ° C, 1.0 ml of enzyme solution was added and stirred, and then 37
After leaving it for 20 minutes at ℃, add 2.0 ml of the reaction stop solution and add 5
Absorbance was measured at 60 nm. In this test, the final concentration of the sample is 0.00476%. The test results of the extracts of Production Examples 1 to 21 are shown in Table 1.

[0053]

[Table 1]

(Usage test) The faces of four women were divided into left and right sides, one of which was used as an example and the other was used as a comparative example every day.
A questionnaire was given three months later after having used it at least once.
In addition, in the comparative example, various extracts of the production examples from the examples are replaced with water. (Comparative Examples 1 and 2) 44 people were 1
Divide into 1 group and use the following Table 2 samples to prevent rough skin,
We conducted an experiment on skin gloss and skin elasticity.

[0055]

[Table 2]

The criteria for judgment are as follows. Table 3 below summarizes the sum of the scores of the four persons as a result of the questionnaire of this score. Example is much better 3 Example is much better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Is much better -3

[0057]

[Table 3]

[0058]

The inhibitory effect of the plant of the present invention is clear from the results of the active oxygen production inhibition rate test, and since it is an extract of the plant, no mutual reaction is considered and two or more species are It is clear that the effect is additive when mixed.
Even in the use test, the effect of preventing rough skin, the shine of the skin, and the effect of retaining the elasticity of the rosemary (manufacturing examples 17 and 18) having a particularly large inhibition rate were large, and these effects were also large (Experiment No. 9) This is supported by the effect of suppressing active oxygen. Since it is a natural product and has been used as a spice for many years, it is guaranteed to be safe for human skin.

Claims (1)

[Claims] 1. Peppermint, oregano, marjoram,
An active oxygen inhibitor comprising a solvent extract of at least one plant leaf selected from the group consisting of thyme, melissa, rosemary and basil.