JP3547155B2 - Active oxygen suppressant - Google Patents

Description

上の試薬の混合物を２．４ｍｌ、検体を０．３ｍｌ加えて、キサンチンオキシナーゼ（予め検体を水とし、実験するとき、吸光度が１分当たり０．０２前後上昇するように上記のリン酸カリウム緩衝液で調整しておく）液を０．３ｍｌ加えて直ちに吸光度（５６０ｎｍ）を測定する。（測定は２分位し、直線性を確認する。）
試算式 阻害率＝（（Ａ−Ｂ）／Ａ）×１００
Ａ：検体を水としたときの１分当たりの吸光度の変化
Ｂ：検体の１分当たりの吸光度の変化
濃度段階を数段階行い、５０％活性酸素生成阻害濃度を探した。検体の作成方法は前記製造例（凍結乾燥品）を適当な濃度の水溶液（溶解しにくい場合はエタノールを加えて溶解したのち精製水を加えて、エバポレートし、エタノールを除去したのち適当な濃度％になるように調製した）とした。その結果を表１に示す。
【００１９】
【表１】

【００２０】
（使用テスト）
女性４名づつの顔面を左右に分け、一方を実施例、もう一方を比較例として毎日、１回以上使用してもらって、３月後、アンケートした。なお、比較例は実施例より製造例の各種の抽出物を水にかえたものである。（比較例１，２）なお、２０名を５班にわけ、下記の表２の試料を使って実験した。
【００２１】
【表２】

判定基準は以下のようでこの評点の合計値をもって評価し、その結果をまとめたのが以下の表３である。
実施例の方が非常によい ３
実施例の方がかなりよい ２
実施例の方がややよい １
差がない ０
比較例の方がややよい −１
比較例の方がかなりよい −２
比較例の方が非常によい −３
【００２２】
【表３】

【００２３】
【発明の効果】
本発明のトラギア インボルクラタのアルコール、水等の溶媒による抽出物は活性酸素抑制試験、使用テストの結果より明らかなように、極く微量でも活性酸素抑制効果が大きく、化粧品に配合した場合、肌荒れを防止し、肌のつや、肌のはりを保持する効果が大きい。長期にわたり、医薬品として使用されてきており、人体に対する安全性が保証されている。[0001]
[Industrial applications]
TECHNICAL FIELD The present invention relates to a plant-derived active oxygen inhibitor that is safe for the human body and can be applied to cosmetics, pharmaceuticals, foods, and the like.
[0002]
[Prior art]
Tragia involculata is a plant belonging to the dicotyledonous net, the leaf-flowering sub-net and the Euphorbiaceae family in India, Sri Lanka, Myanmar and China. In Sri Lanka, it is widely distributed in lowland wasteland. In India, it is used for sweating, laryngitis, bronchitis, pneumonia, leprosy, etc.
[0003]
[Problems to be solved by the invention]
An object of the present invention is to provide an active oxygen inhibitor which is a natural product, which is known to be safe for the human body, and which has a strong active oxygen suppressing effect, and preferably exerts an effect other than the active oxygen suppressing effect. Is to do.
[0004]
[Means for Solving the Problems]
The present inventors have screened and examined plants that have been used for food for many years and have been confirmed to be safe for the human body to solve the above-mentioned problems, and examined those that are useful as active oxygen inhibitors. did. As a result, the solvent extract of preparative Ragia Inborukura data as a cosmetic raw material, or as a quasi-drug, was found to have efficacy in the very active oxygen inhibited.
[0005]
That is, the present invention is an active oxygen inhibitor containing a solvent extract of preparative Ragia Inborukurata (Tragia involucrata).
[0006]
As a method for utilizing these plants, extraction is performed with water or a hydrophilic organic solvent such as ethanol, methanol, or acetone. However, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable because the extraction of raw materials for cosmetics, pharmaceuticals, foods, and the like is performed. In some cases, a polyhydric alcohol such as glycerin, 1,3 butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can also be used for extraction. It is also effective to freeze-dry and use it as a powder depending on the method of use.
[0007]
This material is added with other cosmetic ingredients such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various chemicals. Various dosage forms of cosmetics can be prepared. For example, a use form may be considered depending on the purpose, such as a lotion, a cream, a milky lotion, and a pack.
[0008]
There is oxygen in the air, without which organisms (except anaerobic ones) cannot exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage. In addition, it is said that the generated peroxide and active oxygen damage DNA and accelerate aging. This active oxygen affects the mechanism of producing melanin from tyrosine and is also involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, an important factor required for cosmetics.
[0009]
【Example】
An embodiment which is an actual use method will be described below, but the present invention is not limited to the embodiment. Production examples of extracts of Azadirachdt indica and Tragia involclata used in the present invention and Reference Examples are shown below.
[0010]
(Reference Production Example 1)
300 g of purified water is added to 10 g of the bark of Azadirachdt indica (dry product), and the mixture is heated for 3 hours. This was allowed to cool, filtered, and lyophilized.
[0011]
(Production Example 1 )
300 ml of ethanol was added to 10 g of the whole plant (dried product) of Tragia involculata, and the mixture was allowed to stand for 5 days with occasional stirring. This was filtered and freeze-dried.
[0012]
(Production Example 2 )
To 10 g of a whole plant (dried product) of Tragia involclata was added 300 ml of a 50% aqueous ethanol solution, and the mixture was allowed to stand for 5 days with occasional stirring. This was filtered and freeze-dried.
[0013]
(Production Example 3 )
300 ml of purified water is added to 10 g of the whole plant (dried product) of Tragia involculata, and the mixture is heated for 3 hours. This was allowed to cool, filtered, and lyophilized.
[0014]
(Example 1 ) Cream (% by weight)
A squalane 20.0
Olive oil 2.0
Mink oil 1.0
Jojoba oil 5.0
Beeswax 5.0
Cetostearyl alcohol 2.0
Glycerin monostearate 1.0
Sorbitan monostearate 2.0
Production Example 1 Extract 1.0
B Purified water 47.9
Polyoxyethylene (20EO) sorbitan monostearate 2.0
Polyoxyethylene (60EO) hydrogenated castor oil 1.0
Glycerin 5.0
1.0% aqueous sodium hyaluronate solution 5.0
Methyl paraoxybenzoate 0.1
A and B were each weighed and heated to 70 ° C., A was gradually added to B with stirring, and then cooled to 30 ° C. with slow stirring.
[0015]
(Example 2 ) Lotion (% by weight)
Olive oil 0.5
Production Example 2 Extract 0.5
Polyoxyethylene (20EO) sorbitan monostearate 2.0
Polyoxyethylene (60EO) hydrogenated castor oil 2.0
Ethanol 10.0
1.0% aqueous sodium hyaluronate solution 5.0
Purified water 80.0
[0016]
(Example 3)
Example 3 is a cream prepared by changing the extract of Production Example 1 of Example 1 to the extract of Production Example 3.
[0017]
(Reference Example 1)
Reference Example 1 is a lotion prepared by changing the extract of Production Example 2 of Example 2 to the extract of Reference Production Example 1.
[0018]
(Active oxygen suppression test)
There are various methods for measuring the effect of suppressing active oxygen, but the following method was used this time.

Add 2.4 ml of the mixture of the above reagents and 0.3 ml of the sample, and add xanthine oxynase (the above-mentioned potassium phosphate so that the absorbance increases by about 0.02 per minute when the sample is made into water before the experiment). Add 0.3 ml of the solution (adjusted with a buffer solution) and immediately measure the absorbance (560 nm). (Measurement is divided into two and linearity is confirmed.)
Trial calculation formula Inhibition rate = ((AB) / A) × 100
A: Change in absorbance per minute when the sample was water B: Change in absorbance per minute of the sample Several steps were performed to find the 50% active oxygen production inhibitory concentration. A sample was prepared by dissolving the above-mentioned production example (freeze-dried product) in an aqueous solution having an appropriate concentration (if it was difficult to dissolve, adding ethanol to the solution, adding purified water, evaporating the solution, removing the ethanol, and then removing an appropriate concentration of Was prepared so that Table 1 shows the results.
[0019]
[Table 1]

[0020]
(Use test)
The face of each of four women was divided into left and right, one was used as an example and the other was used as a comparative example, and used once or more every day. In the comparative examples, various extracts of the production examples were replaced with water from the examples. (Comparative Examples 1 and 2) Twenty people were divided into five groups, and experiments were performed using the samples shown in Table 2 below.
[0021]
[Table 2]

The criterion is as follows, and the evaluation was made based on the total value of the scores, and the results are summarized in Table 3 below.
Example is much better 3
Example is considerably better 2
Example is slightly better 1
No difference 0
Comparative example is slightly better -1
Comparative example is much better -2
Comparative example is much better -3
[0022]
[Table 3]

[0023]
【The invention's effect】
DOO Ragia Inborukura other alcohols, extract with solvents such as water of the present invention the active oxygen inhibition test, as is clear from the results of use tests, large active oxygen inhibitory effect in trace amount, when incorporated in cosmetics, It has a great effect of preventing rough skin and keeping skin shine and skin scalp. It has been used as a pharmaceutical for a long time, and its safety to the human body is guaranteed.

Claims (1)

Active oxygen inhibitor containing a solvent extract of preparative Ragia Inborukurata (Tragia involucrata).