JPH04164013A - Skin, scalp and head hair-treating agent containing ingredient capable of preventing growth of pityrosporum ovale - Google Patents

Abstract

PURPOSE:To obtain a cosmetic containing an extract obtained by extraction from a leaf or bark of Melia azadirachta L. using a specific solvent, having strong action capable of inhibiting growth of Melia azadirachta L. and useful for prevention of dander and pruritus. CONSTITUTION:A leaf or/and bark of Melia azadirachta L. is subjected to extraction treatment with either one or kind of benzene, chloroform, ether, ethyl acetate, n-butanol, acetone, ethanol and methanol to provide extract having action capable of inhibiting growth of Melia azadirachta L. The extract is blended into cosmetic formulation at an amount of 0.1-0.5%. The extract is useful as an antioxidant for oils and fats simultaneously blended in cosmetics as well as also as a stabilizer for cosmetic product and further may have action suppressing production of a peroxide lipid.

Description

DETAILED DESCRIPTION OF THE INVENTION Object of the invention
azadirachta L, (=Azadirach
hta 1ndica A, Juss: Neem or M
argosal] leaves and bark.

The dandruff bacteria in the present invention belong to yeast bacteria that are known to reside permanently on the scalp. Pityrosporum ovale: Hereinafter,
For convenience, it is abbreviated as PO).

"Industrial Application Fields" The extract containing the PO growth-inhibiting component obtained by extracting from neem of the present invention can be used in pharmaceuticals, quasi-drugs, and cosmetics in the form of external application and rubbing. It can be used by incorporating it into the formulation of PO, and can prevent, prevent, and improve the onset of dandruff caused by PO and the accompanying itching.

``Conventional technology'' Various causes have been cited for the development of dandruff and itching on the skin and scalp, but one of them is closely related to PO, which is resident on the scalp. It is said that the presence rate is particularly high from adolescence to adulthood, and consists of sweat gland secretions, peeled substances from the epidermal layer, etc., and usually occurs due to increased secretion of sebaceous glands, etc. It is also said to be promoted by the proliferation of bacteria on the skin, especially PO.

On the other hand, when looking at the relationship between the onset of dandruff and the itching that accompanies dandruff and the ability to secrete sebum, we find that triglycerides in sebum increase the production of lipase due to the entry of PO into the hair follicle. , the production of chrycerol and free fatty acids is increased, and the excess free fatty acids produced at this time have a side-forming effect and an inflammation-inducing effect, especially,
Strong (I know I will be seen.

Originally, PO is lipid-dependent and lipophilic, so as the amount of sebum increases and accumulates in the hair follicle canal, PO also gradually increases. Factors such as neutrophil chemotaxis by PO, complement activation factors, and the products of these bacteria, such as protease, lipase, and hyaluronidase, genetic factors, age, 1 meal, 1 ■ Dandruff is thought to be caused by a complex interrelationship of internal and external factors such as physical factors, gastrointestinal disorders, stress, menstrual irregularities, mechanical stimulation, and cosmetics. In the mechanism of development of itching, prevention of PO inhabitation (invasion and growth) in the lesion is considered to be very important in the prevention and treatment of dandruff and itching.

Therefore, recently, if a component that inhibits or suppresses its growth is discovered, it is considered to be useful for prevention and treatment.

Substances that have a growth-inhibiting effect on PO include zinc pyrithione and alkylisoquinolium bromide solution, and plant-derived ingredients include extracts of Japanese cabbage, stone wax, kujin, and chamomile (reported by Yoshimasa et al., literature available). : Cosmetic Magazine, Vol. 22, No. 3, 1988), and among these, the present inventors have so far developed a method for preventing dandruff and itching. In comparison, ingredients that have a growth inhibiting effect on PO,
There is a history of pursuing research in search of natural materials (see Table 1 below).

The research topic is to extract PO from extracts derived from each plant.
Our goal is to provide an effective growth-inhibiting ingredient to the human body, and at the same time, we are investigating simple and efficient methods to obtain the desired product, and are striving to apply it to pharmaceuticals, quasi-drugs, cosmetics, etc. Ta.

``Problems to be Solved by the Invention'' As mentioned above, the present inventors have discovered that effective growth-inhibiting ingredients can be easily and efficiently extracted from various types of plants, and other extracts can be extracted from the skin, scalp, etc. One purpose is to use it in cosmetics (cosmetics) in the form of external application and rubbing on the hair.
In order to achieve this goal, we investigated extraction solvents from various angles and pursued the best specific solvent.

"Means for Solving the Problem" In other words, we obtained various plants and screened the extracts of each plant to search for and evaluate components that inhibit PO growth.

As a result, it was found that an extract obtained from the leaves and/or bark of neem contains a component that inhibits PO growth.

In India, neem is called neem and has been known as a folk medicine since ancient times, and is heavily used as a bitter tonic, astringent, and as a medicine for periodic fevers.
As a result of trying to extract components that have an excellent PO growth inhibiting effect from various angles, we found that the extract obtained by simple extraction using the specified extraction solvent has a strong PO growth inhibiting effect, as shown below. It was discovered that the component exists.

Hereinafter, extraction examples, comparative examples, etc. will be disclosed and described in more detail.

The method shown below shows an extraction example with a strong effect in the confirmation test described later, but uses crushed neem bark and/or leaves and uses benzene, chloroform, It was found that any extract obtained using ether, ethyl acetate, n-butanol, acetone, ethanol, or methanol can have similar effects. .

Furthermore, it may be an extract obtained by mixing one or more of the extraction solvents specified in the present invention, and the form of the extract may be, for example, a solution diluted in ethanol, or may be powdered or pasted.

The extract obtained here can be dissolved in a mixture of ethanol and water, or added to a wet solution containing a polyol solvent (e.g., glycerin, propylene glycol, butylene glycol). Once dissolved, it can be easily incorporated into liquid-type product formulations.

(Extraction Example 1) To I kg of crushed neem leaves, 10 ρ of chloroform is added, and the mixture is left to stand for three days and nights at room temperature, and then filtered to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain a PO growth inhibition-containing extract.

(Extraction Example 2) To I kg of crushed neem bark, 10 I2 of chloroform is added and left at room temperature for three days and nights, and then filtered to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain a PO growth inhibition-containing extract.

(Extraction example 3) For IKg of crushed neem leaves.

After adding ethyl acetate 10I2 and leaving it for three days and nights at room temperature, it is filtered to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain an extract containing a PO growth-inhibiting component.

(Extraction Example 4) After adding 10I2 of ethyl acetate to Ikg of crushed neem bark and leaving it at room temperature for three days and nights,
Filter and separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain a PO growth inhibition-containing extract.

(Extraction example 5) For IKg of crushed neem leaves.

Acetone 10I2 is added and the mixture is left at room temperature for three days and nights, and then filtered to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain a PO growth inhibition-containing extract.

(Extraction Example 6) Acetone 10β is added to I kg of crushed neem bark, and after being left at room temperature for three days and nights, it is filtered to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain a PO growth inhibition-containing extract.

(Extraction Example 7) 10 grams of methanol is added to 1 kg of crushed neem leaves, and the mixture is left to stand for three days and nights at room temperature, and then filtered to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of ethanol to obtain a PO growth inhibition-containing extract.

(Extraction Example 8) 1012 methanol was added to 1 kg of crushed neem bark, and the mixture was left at room temperature for three days and nights.
Filter and separate the extract.

Next, after concentrating this extract and distilling off the solvent, a small amount of
Fi is dissolved in ethanol to obtain an extract containing I) O growth-inhibiting acids.

(Comparative Example 1) 1 kg of crushed neem bark to 1 kg of water
After adding water and extracting at 80 to 90°C for 4 hours, the extract is collected by filtration.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of aqueous ethanol to obtain an extract containing a PO growth-inhibiting component.

(Comparative Example 2) 1 kg of crushed neem leaves was mixed with 15 f of water.
After adding f and extracting at 80 to 90°C for 4 hours, filter the mixture to separate the extract.

Next, this extract is concentrated to remove the solvent, and then dissolved in a small amount of aqueous ethanol to obtain an extract containing a PO growth-inhibiting component.

[Confirmation test for inhibitory effect (effect) on the growth of Vityrosborum ovale bacteria] <a) Preparation of Vityrosborum ovale suspension
Cultured on potato dextrose agar medium containing 5% Nikkor TMGO-5 (manufactured by Nikko Chemical Co., Ltd.) (manufactured by Tsume 1), removed 3 platinum loops, and cultured in physiological saline containing 0.7% Twin 80.
mj2 and suspended in a manner similar to that of 660 nm.
This suspension prepared with physiological saline was again diluted 100 times with physiological saline so that the absorbance was 0.1ABS, and the suspension was diluted 100 times with the physiological saline. Turbid liquid (P.

0 liquid).

<b>Test method A test tube containing a certain amount of sample was mixed with dimethyl sulfoside (DMSO) 0,5 m! as a solvent. Alternatively, add 0.5 mL of water to prepare a sample solution with a final concentration of 0.1%, and place this sample solution in a sterilized petri dish.
Next, a potato dextrose agar medium (diameter 90 mm) containing 5% Nikkor TMGO-5 is added to a sterilized petri dish, spread, and left at room temperature to prepare a plate.

Separately, 0.2 mρ of the above Vityrosvorum oberle suspension (P, O solution) was placed in a test tube, and the temperature was further increased at 45°C.
In addition to 3 m12 of soft agar that has been dissolved and preserved in
After culturing at 0°C for 2 days, the growth state was observed.

A control without the sample solution was used, and a commercially available 50% zinc pyrithione solution was used as a comparison sample (added to give a final concentration of 0.1% in the same way as the sample solution).

<c>Grade results In Table 2, the growth inhibitory effect on PO was shown.

The sample containing the extract component of the present invention had almost the same results as the comparative sample zinc pyrithione at a concentration of 0.1%.

(Approximate amount to be added) The neem extract according to the present invention can be effective as a PO growth inhibiting ingredient by adding a small amount to the formulation.
It can be used as a compounding agent to prevent dandruff and itching.1 The usual amount is 11 to 0.5% of O in the formulation, and depending on the extract, it is about 0.01 to 0.05%. There are some things that can be expected to be effective.

In addition, the PO growth inhibiting ingredient comprising the neem extract according to the present invention can be used in all cosmetics, such as solution-type products such as shampoos for hair and scalp, conditioners, hair tonics, hair creams, hair ticks, and hair care products. It can be incorporated into the formulation of cosmetic products having various dosage forms such as pomades, skin lotions, creams, and emulsions.

For specific formulations, see the following publications:
Since prescription examples corresponding to various uses have been presented, it is sufficient to follow their manufacturing methods.

[Location of references in prescription]

Science of Fragrances and Cosmetics: Back 1) Haru et al. / October 5, 1980: Published by Koyo Shoten "Confirmation test of anti-dandruff and anti-itch effect" (a) Test method For testing, first, the following table ( In the hair shampoo formulation as shown in Table 3, we added the extract containing the PO growth-inhibiting component obtained in the above (manufacturing/extraction example) and the hair shampoo without additives. We selected 20 people, including those who tend to have dandruff on their hair, and those who suffer from dandruff and itching, to compare and evaluate the effects after using it for about two weeks.

The results were summarized as shown in the following tables (Tables 4 and 5).

That is, the numbers shown in Tables 4 and 5 are the results of the responses received regarding the effects of using each hair shampoo, expressed in terms of the number of people.

(b> Results As shown in Table 4, the hair shampoo containing the extract: PO growth inhibiting ingredient according to the present invention is clearly effective in suppressing dandruff, even when compared to hair shampoo without additives. Estimated.

Furthermore, as shown in Table 5, it was estimated that the hair shampoo containing the extract of the present invention: PO growth-inhibiting component had a suppressing and alleviating effect on itching compared to the hair shampoo containing no additives.

"Table 3" Hair shampoo formulation "Effects of the invention" The present invention discovered that an extract of a specific extraction solvent from the leaves and/or bark of neem has a prominent PO growth inhibiting effect, and utilizes it. The point is that we have made it possible.

That is, the expected effects of using the extract obtained from the leaves and/or bark of neem according to the present invention in external preparations for the skin, scalp, and hair are as follows. It can be summarized.

■Helpful for the prevention and treatment of dandruff and itching on the skin and scalp.

■It is possible to prevent not only dandruff and itching, but also seborrheic eczema and hair loss on the head, and is thought to promote blood flow in the peripheral blood vessels of the scalp and skin. ■For skin or hair.
When formulating cosmetics, we freely combine known and commonly used ingredients such as surfactants, humectants, lower alcohols, thickeners, fragrances, antioxidants, chelating agents, pigments, and preservatives as necessary. Can be used.

(2) When the neem extract according to the present invention is blended in a small amount, at the same time it exerts an antioxidant effect on the oils and fats in preparations containing various lipids in the formulation.

Furthermore, the feature of the extract containing the PO growth-harming component according to the present invention is that it is useful as a stabilizer for cosmetic products, and it is also thought to suppress the production of lipid peroxide, so it may be useful for skin beautification, etc. It also helps maintain skin (prevents skin aging).
It is also possible to powder the extract and use it as an additive for foods and the like.

Claims (1)

[Claims] (1) Using neem leaves and/or bark as a starting material,
Benzene, chloroform, ether, ethyl acetate, n-
Extracted using one or more of the organic solvents of butanol, acetone, ethanol, and methanol,
A preparation for the skin, scalp, and hair, characterized by containing a component that inhibits the growth of dandruff bacteria.