KR102093708B1 - A cosmetic composition using Fraxini cortex extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a chimpanzee extract as a main active ingredient, more specifically, extract the ash tree bark using a solvent, concentrate and freeze-dried the chimpanzee extract prepared by 0.001 ~ 1.0% by weight It relates to a cosmetic composition containing excellent sebum secretion inhibitory effect, acne prevention effect, hair damage prevention effect, hair loss prevention and hair growth effect.

Description

A cosmetic composition using Fraxini cortex extract}

The present invention relates to a cosmetic composition containing a chimney (Fraxini Cortex) extract, which is a bark of the ash family (Frixinus rhynchophylla ) as a main active ingredient, and more specifically, the chimney extract as an active ingredient in an amount of 0.001 to 1.0% by weight. It relates to a cosmetic composition that is characterized in that it contains, and suppresses sebum secreted by excess of the skin, excellent in preventing acne, preventing hair damage, preventing hair loss, and hair growth.

The Fraxini cortex is a dried bark of the ash tree ( Fraxinus rhynchopylla ). The ash tree is a deciduous tree. It is a tall tree that grows up to 15 m in height and 60 cm in diameter. It is also called Simmok and Cheongpimok. The origin of the name is that the water turns blue when you peel off the bark and is named ash tree. The leaves are opposite to each other, and consist of 4 to 8 leaves. Two or more small leaves are arranged on both sides of the left and right sides of the extension of the leaf tip to form the feathers of a bird. It is lanceolate about 15cm long. It is characterized by having hair on the back, but not on the back. In Chinese medicine, the bark is used as a medicine called chimpi (피), and is used as a medicine for neuralgia, hemoptysis, and edema. It is known that bark contains components such as esculin, mannitol, fraxin, esculetin, and fraxetin.

The bark extract, which is the bark of the ash tree, is a technology related to anti-inflammatory, skin irritation, and collagen biosynthesis promoting effects. "Skin external preparation containing dermis extract as the main active ingredient" (Korean Patent Publication No. 10-0732564, Patent) Literature 1) has been published, but there has been no report on sebum suppression and acne prevention effects and hair loss prevention and hair growth effects.

 Since male pattern alopecia is male hormone-dependent, it is directly related to the amount of male hormone, and accordingly, many studies have been reported for the prevention and treatment of hair loss by inhibiting male hormone activity. On the other hand, when the function of the sebaceous gland becomes active due to the increase in the secretion of male hormone, the cotton fabric generated as the excess sebum produced stagnates in the hair follicle due to the hyperkeratization of the follicle wall is the initial stage of acne. When explaining the mechanism of male hair loss and acne, 5-alpha-reductase is one of the male hormones present in testosterone-responsive tissues such as sebaceous glands, hair follicles, prostate, and epididymis. , It is an enzyme involved in metabolizing testosterone to dihydrotestosterone, and NADPH is required for its conversion. In addition, testosterone is involved in male sex drive, skeletal muscle increase, male genital organs, scrotum growth, spermatogenesis, etc., and dihydrotestosterone is involved in relevant tissues such as acne, sebum increase, hair loss and prostate hyperplasia. In particular, after puberty, excessive secretion of testosterone causes acne and hair loss. 5-alpha-reducta is used to prevent overproduction of dihydrotestosterone, an active form of testosterone produced by 5-alpha-reductase. Researches have been actively undertaken to develop inhibitors of hyperphobia, anti-hair loss, and anti-acne agents using azeotropic inhibitors.

In order to solve this problem of skin, many cosmetics using natural products have been developed to reduce skin irritation due to various chemicals. Natural ingredients have fewer side effects on the skin, and recently, as consumers' response to cosmetics using natural ingredients has increased, the development value of cosmetics has increased.

KR 10-0732564 (2007.06.20)

An object of the present invention is to provide a cosmetic composition containing a chimpanzee extract.

More specifically, the object of the present invention is a cosmetic composition that can be applied to a cosmetic and contains a natural extract having an effect of inhibiting excessive sebum secretion, preventing acne, preventing hair damage, preventing hair loss, and promoting hair growth when contained in a cosmetic. Is to provide.

The cosmetic composition using the chimpanzee extract of the present invention is characterized in that it contains the chimpi extract having an inhibitory effect on the activity of 5 alpha-reductase as an active ingredient in order to solve the above problems.

In the above configuration, the chimney extract is characterized in that it contains 0.001 to 1.0% by weight relative to the entire cosmetic composition.

In addition, the chimney extract is characterized in that it is extracted using at least one solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane or hexane.

In addition, the chimney extract is characterized by extracting ash bark at room temperature, cooling and filtering, and then concentrated under reduced pressure and freeze-dried at 50 ° C or lower.

In addition, the cosmetic composition is characterized in that for suppressing excessive sebum secretion.

Or, the cosmetic composition is characterized in that for preventing acne.

In addition, the cosmetic composition is characterized in that for preventing hair damage.

In addition, the cosmetic composition is characterized in that it is for hair loss prevention and hair growth.

The cosmetic composition formulation of the present invention includes lotion, gel, water-soluble liquid, cream, ointment, essence, shampoo, hair rinse, hair treatment, hair mousse, lipstick, oil-in-water (O / W) or water-in-oil (W / O) It is characterized in that it is selected from cosmetic compositions of the type.

Or, the purified water is dissolved in glycerin and chimpanzee extract, and is composed of ethanol and seedling plant fermentation broth, based on 100 parts by weight of ethanol is composed of 9.4 to 9.5 parts by weight, the chimney extract is based on 100 parts by weight of purified water It is composed of 0.057 ~ 0.059 parts by weight, the myochoweed fermentation broth is composed of 1.18 ~ 1.19 parts by weight based on 100 parts by weight of purified water, and the chimney extract, 70% (v / v / v) ) After reflux extraction with an ethanol aqueous solution for 6 hours, cooled, filtered with filter paper, and the filtered extract is prepared by concentrating under reduced pressure and freeze-drying at 50 ° C. or less, and the seedling water fermentation broth, after obtaining a seedling leaf, It is characterized by being prepared by mixing seedlings, salt, and vinegar at a ratio of 1: 3 and then fermenting for 1 month, and obtaining only the supernatant from which the solids are separated. The.

The chimney extract according to the present invention exhibited an inhibitory effect on excessive sebum secretion by inhibiting the activity of 5-alpha-reductase, which is closely related to male hormone production.

In addition, the chimpanzee extract exhibited antibacterial activity effects for acne bacteria having anti-hair loss and anti-acne effects.

Therefore, it can be seen that cosmetic compositions such as lotions, creams, emulsions, packs, powders, etc., containing such a chimney extract have excellent skin external agent effects such as acne prevention, hair damage prevention, hair loss prevention, and hair growth effect.

The inventors of the present application, after a long study, selected the ash tree chimney and prepared an extract therefrom, the result of measuring excessive sebum secretion suppression effect, acne prevention effect, hair damage prevention effect, hair loss prevention, and hair growth effect is excellent. Therefore, it was discovered that the efficacy as a cosmetic can be expected.

Accordingly, the present invention provides a cosmetic composition containing a chimney extract.

In addition, the chimney extract of the present invention is characterized in that it contains 0.001 to 1.0% by weight based on the freeze-dried weight with respect to the entire cosmetic composition. When the content of the extract is less than 0.001% by weight, there is almost no skin improvement effect, and when it is 1.0% by weight or more, it has cytotoxicity, and the degree of increase in the effect on the content increase is not economical.

In addition, the present invention is characterized in that the extraction solvent of the chimney extract is extracted using at least one solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane.

In addition, the present invention is characterized in that the chimney extract is extracted at room temperature, cooled and filtered, then concentrated under reduced pressure and freeze-dried at 50 ° C or less.

In addition, the present invention is the cosmetic composition is a lotion, gel, water-soluble liquid, cream, ointment, essence, shampoo, hair rinse, hair treatment, hair mousse, lipstick, oil-in-water (O / W) type and water-in-oil (W / It is characterized by being selected from O) type formulations.

In addition, the chimney extract of the present invention can be formulated into pharmaceutical compositions such as capsules, liquids, ointments, patch or sustained release preparations using a pharmaceutically acceptable carrier, pharmacologically acceptable bases, carriers, Excipients, binders (e.g. starch, tragacanth rubber, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose), crushing agents ( Examples: Agar, starch, bellatine powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium hydrogencarbonate and sodium alginate), lubricants (e.g. magnesium stearate, talc, hydrogenated vegetable oil) and coloring agents Includes. Lactose, glucose, sucrose, mannitol, potato starch, cornstarch, calcium carbonate, calcium phosphate and cellulose are used as the carrier and excipients.

In addition to the above, additives such as stabilizers, dissolution aids, adjuvant fragrances such as transdermal absorption accelerators, and preservatives may be further added.

The pharmaceutical composition prepared as described above may be applied to the skin once or several times a day depending on the symptoms, and the application may be adjusted according to the degree of improvement of symptoms.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are merely illustrative of the present invention, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of chimney extract

After slicing, the stem bark of the shaded ash tree was refluxed for 6 hours in 70% (v / v) ethanol aqueous solution in purified water, cooled, and then filtered with Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C or lower to prepare a powdery extract.

Example 2: Evaluation test of 5 alpha-reductase activity inhibitory activity of chimney extract

In this example, 5 alpha-reductase activity inhibitory powers were evaluated to determine the anti-hair loss and anti-acne effects of the chimpanzee extract obtained in Example 1. 5 Alpha-reductase activity inhibition The 5 alpha-reductase used in the experiment was an enzyme produced by fibroblast-derived fibroblasts.

Fibroblasts were cultured after inoculation with 10,000 cells per microplate hole. After adding 0.1.mμ.Ci of testosterone radiolabeled with 3H (tritium) for each hole, it was cultured to measure whether fibroblasts use it. As a control, what did not contain the chimpanzee extract was used. After incubation for 24 hours, a supernatant is obtained, and steroids are obtained with 1 ml of ethyl acetate-cyclohexane 1: 1 extraction solvent. The obtained steroid is placed on a thin-film chromatography plate and developed with a mixture of chloroform / methanol 98/2 (v / v). The radioactivity at the points corresponding to testosterone and dihydrotestosterone was measured using a densitometer to calculate the conversion rate, and the result was compared with the control group (conversion rate when no extract was added) by Equation 1 below. The ability to inhibit alpha-reductase was evaluated.

<Equation 1>

Inhibitory capacity (%) = (A-B) / A × 100

[A = Testosterone to dihydrotestosterone conversion rate (without extract)]

[B = Conversion rate from testosterone to dihydrotestosterone (when extract is added)]

As a result of the calculation according to the above equation, it can be seen that the chimney extract has a 5 alpha-reductase inhibitory effect as shown in Table 1 below.

Concentration of chimpanzee extract of Example 1 (%) 0.01 0.005 0.0025 0.001 0.0005 0.00025 0.0001 Inhibition rate (%) 88.2 78.5 63.1 58.2 41.7 30.2 16.5

Example 3: Hair growth effect test

In this example, a 30% hydrous ethanol solution containing 2% dry extract of chimney extract in Example 1 was prepared and used in the test. For the hair growth effect test, the hair of the back was removed using a mouse (ICR) 47-53 days old, the clean back was selected, and 10 animals were used for each substance group. Each was applied. The length of the hair and the degree of hair growth over time were compared by adding scores according to the degree of restoration after hair removal. In order to compare the degree of hair growth, as a control, a growth state was observed by applying a 30% alcohol solution to each individual.

Days / sample 5 10 15 Chimpi extract of Example 1 0.15 mm 0.96 mm 1.95 ± 0.25mm Control 0.06 mm 0.19 mm 0.140.85 ± mm

Example 4: Measurement of antibacterial activity against acne bacteria

This example was carried out a paper disc test (Paper Disc Test) for confirming the antibacterial activity of acne bacteria of the chimpanzee extract obtained in Example 1. First, pre-incubation was performed in BHI liquid medium (Brain-Heart Infusion Broth; 3.7%) for 48 hours in order to activate Propionibacterium acnes, which is the cause of acne. The prepared bacterial culture solution was applied to BHI solid medium (Brain-Heart Infusion Broth; 3.7%; agar 1.5%) 0.1 ml each, and then dried. After diluting the chimney extract obtained in Example 1 with 12% (w / v) in 95% ethanol aqueous solution, dropping 50 µl each onto a paper disc having a diameter of 8 mm and placing it on the prepared solid medium, and then 35 µl anaerobic it Incubated in the culture tank for 3 days. Antibacterial activity was evaluated by observing the area of growth inhibition around the paper disc and measuring the size of the ring.

As a result of measuring the antibacterial activity against acne bacteria, it was found that the size of the growth inhibition ring of the chimney extract was 24 mm.

Example 5: Sebum secretion suppression and acne improvement effect

In this example, the cosmetic composition containing the chimney extract obtained in Example 1 was prepared, and the effect of inhibiting sebum secretion and acne improvement was evaluated by performing comparative experiments with Comparative Example 1.

The cosmetic used in the comparative experiment is in the form of a cream, and the composition is shown in Table 3. First, the phase B) recorded in Table 3 is heated and stored at 70 ° C. To this, a) phase was added, followed by pre-emulsification, uniformly emulsified with a homomixer, and then slowly cooled to prepare a cream (Example 5, Comparative Example 1). The experiment was conducted by dividing into two groups A and B for 10 experimenters (men and women aged 20-35). In Group A, the cream prepared in Example 5 containing the chimney extract, and in Group B, the cream prepared in Comparative Example 1 was used every morning and evening for 4 weeks after cleansing.

For sebum measurement, the blood was stabilized in a constant temperature and humidity chamber for 30 minutes after washing, and sebum was measured in the forehead using a sebum meter. Before using the cream containing the chimney extract (Example 5), the sebum amount was set as the initial value, and the change in sebum amount was measured once every week for 4 weeks. As shown in Table 4, it can be seen that the experimenter who applied the cream containing the chimney extract effectively reduced sebum secretion over time.

Raw material Example 5 Comparative Example 1 end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic acid cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol parts
A) Alcohol ester 3 3 Glyceryl monostearic acid
Aster 2 2 I Chimney extract One - Propylene glycol 5 5 Proper Proper Proper

Note) Unit:% by weight

Cycle Sebum volume (g / cm ² / h) Example 5 Comparative Example 1 Initial value 122.2 ± 10.2 134.1 ± 15.3 1 week 121.4 ± 13.3 130.4 ± 23.5 2 weeks 112.7 ± 15.4 135.7 ± 12.1 3 weeks 105.6 ± 22.1 138.2 ± 28.8 4 weeks 101.2 ± 14.7 131.3 ± 14.2

The cream prepared in Example 5 was applied to the entire face for 4 weeks in the morning and evening in 10 subjects who had acne (20-35 years old male and female). The acne improvement effect was evaluated by evaluating the condition before the test with 1-3 points based on the emotions of the experimenter himself, and the results are shown in Table 5.

Tester Pre-test status After 2 weeks of testing After 4 weeks of testing One One + ++ 2 2 - - 3 2 + + 4 2 - + 5 One - + 6 3 ++ +++ 7 2 - + 8 2 + ++ 9 3 + ++ 10 One + ++ <Evaluation of state before test>
1 = some acne 2 = some acne 3 = severe acne
<Acne effect judgment>
-: Almost no effect +: little effect ++: much relief
+++: Healed completely

From the results of Table 5 above, it was possible to confirm the acne improvement effect in the testers using the cream containing the chimney extract of the present invention.

Example 6: Skin irritation relief effect

In this experimental example, the effect of alleviating irritation of a cosmetic containing the chimney extract obtained in Example 1 was evaluated by a human patch test.

1% of sodium lauryl sulfate (SLS) that causes irritation to the general cosmetic prescription (cream, lotion, skin, essence) and the product prepared in Example 5 are mixed and patched for 24 hours, 48 hours, and 72 hours to obtain an irritation-inducing index. Based on this, the effect of stimulation is evaluated.

Each of the 50-50 healthy males and females aged 20-50 years old was patched with 0.3 mg of each product using FINN CHAMBER (FINLAND), and the acute irritation index was evaluated after 24 hours. After evaluation, the same amount of product was applied to the same site again, and the delayed irritation index after 48 hours and 72 hours was evaluated.

As a result of the test, the part where the SLS was applied alone showed a red irritation on the skin after 24 hours, but it did not cause any skin seizure even after 24 hours, 48 hours, and 72 hours elapsed after application of the product containing the chimney extract.

The results of this evaluation showed that the chimpanzee extract has a significant effect in reducing skin irritation caused by substrates (surfactants, fragrances, alcohols) that cause irritation when used in cosmetics.

Other examples are shown below. That is, the shampoo, lotion, emulsion and cosmetic liquid containing the chimpanzee extract obtained in Example 1 were prepared in Examples 7 to 10. Shampoos, lotions, emulsions and cosmetic liquids containing these chimney extracts showed excellent effects in skin improvement such as excessive sebum secretion suppression effect, acne prevention effect, hair damage prevention effect, hair loss prevention and hair growth effect.

Example 7: Preparation of shampoo containing chimney extract

5 g of 95% ethanol was mixed with 5 g of ammonium laureth sulfate, 5 g of cosylbetaine, 0.5 g of alkyl ether sulfate, 0.5 g of silicone, 0.2 g of polyquarter, 0.2 g of fragrance, and 0.02 g of preservative. 5 g of the chimney extract of the present invention obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and the mixture was added and stirred to obtain a shampoo.

Example 8: Preparation of lotion containing chimney extract

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are mixed and dissolved. . 0.05 g of the chimney extract obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and the mixture was added and stirred to obtain a lotion.

Example 9: Preparation of emulsion containing chimney extract

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesterade, 1 g of polyoxyethylene (addition of 20 mol) monooleate, and 0.1 g of fragrance were heated and dissolved at 70 ° C. , 0.5 g of chimney extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. The mixture was emulsified by mixing and cooled to obtain an oil-in-water (O / W) emulsion.

Example 10: Preparation of cosmetic liquid containing chimney extract

Polyoxyethylene sorbitan monooleate 1.2g, chitulose 0.3g, sodium hyaluronate 0.2g, vitamin E-acetate 0.2g, sodium licorice 0.2g, paraoxybenzoic acid ethyl ester 0.1g, 5g 95% ethyl alcohol 1 g of the chimney extract obtained in Example 1 and an appropriate amount of pigment were mixed to obtain a cosmetic liquid.

Example 11: Preparation of lotion containing chimney extract 2

Prepared as in Example 8, was prepared by further adding the myochoweed fermentation broth.

Myocho is a scientific name of Orthosiphon stamineus Bentham, and is generally called "Cat Whiskers". It is also called cat whiskers in Korean.

The region where it grows mainly is Indonesia.

On the other hand, there have been no reports of using myocho as a raw material for hair-related lotion.

The myochoweed fermentation broth was used to obtain a myochoweed leaf, then mix myochoweed, salt, and vinegar in a ratio of 1: 3: 1, then ferment for 1 month, and obtain only the supernatant from which solids were separated.

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are mixed and dissolved. . 0.05 g of the chimpanzee extract obtained in Example 1 and 5 g of glycerin were dissolved in 84.33 g of purified water, and 1 g of the myochoweed fermentation broth was added to the mixture, followed by stirring to obtain a lotion.

After soaking the hair in the prepared lotion for 20 minutes, the tensile strength of the hair was measured.

In this case, a digital force gauge (DPS-5R) was used to measure the tensile strength of the hair, and the measurement was performed 10 times.

For comparison, the lotion of Example 8 was compared with pure hair immersed for 20 minutes.

As a result of the measurement, the tensile strength of the hair immersed in the lotion of Example 11 was measured to be 0.114 kg, while the tensile strength of the hair dipped in pure water was measured to be 0.091 kg.

For reference, in Example 8, the tensile strength of the hair was measured to be 0.106 kg.

Therefore, it can be seen that the chimpanzee extract of the present invention has an effect of improving the tensile strength of hair, and when the fermentation broth of the myelin sheath is added thereto, the effect is further increased.

Claims (10)

delete delete delete delete delete delete delete delete delete In the cosmetic composition,
Consists of purified water in which glycerin and chimney extract are dissolved, and ethanol and seedling plant fermentation broth.
Ethanol is composed of 9.4 to 9.5 parts by weight based on 100 parts by weight of the purified water,
The chimney extract is composed of 0.057 to 0.059 parts by weight based on 100 parts by weight of purified water,
The myochoweed fermentation broth is composed of 1.18 to 1.19 parts by weight based on 100 parts by weight of purified water,
The chimney extract is excised, refluxed the stem bark of a shaded ash tree into purified water with 70% (v / v) ethanol aqueous solution for 6 hours, cooled, filtered with filter paper, and the filtered extract was below 50 ° C. It is prepared by concentration under reduced pressure and lyophilization,
The myochoweed fermentation broth is prepared by obtaining a myochoweed leaf, mixing myochoweed, salt, and vinegar in a ratio of 1: 3 and then fermenting for 1 month, and obtaining only the supernatant from which the solids are separated. Made with,
Cosmetic composition using chimney extract.