JP6417661B2 - Speculum plate - Google Patents

Description

  The present invention relates to a spectroscopic plate, and more particularly to a spectroscopic plate that is used when a liquid sample is inspected using a microscope and includes a liquid sample storage section that fills the liquid sample.

  Conventionally, when using a microscope to examine formed components such as blood cells, epithelial cells, columnars, microorganisms, and crystalline salts contained in liquid samples such as urine residue components, slide glass After the liquid sample was dropped on the cover glass, the cover glass was placed on the liquid sample and observed with a microscope. By simply placing the cover glass on the liquid sample, the distance between the cover glass and the slide glass is not constant, and the amount of liquid sample that is measured varies depending on the amount of liquid sample that falls within the predetermined observation field of view. There was a case where the number of scatters.

  Therefore, in order to suppress variation in the amount of liquid sample that fits in a predetermined visual field, various spectroscopic plates in which a cover glass is fixed in advance to a slide glass at a predetermined interval have been developed.

  As literatures in which such speculum plates are disclosed, for example, JP-A-2002-350739 (Patent Document 1) and JP-A-2006-2013367 (Patent Document 2) can be cited.

  The spectroscopic plate disclosed in Patent Document 1 includes a plate-like slide glass portion and a slide glass (second plate-like portion) including a concave portion or a convex portion formed on one main surface of the slide glass portion, and the slide. A plate-like cover glass portion facing the glass portion, and a cover glass (first plate-like portion) including a convex portion or a concave portion that fits into the concave portion or the convex portion formed on the main surface of the cover glass portion. The cover glass is fixed with a certain distance from the slide glass by fitting the convex or concave portion of the cover glass into the concave or convex portion of the slide glass.

  The spectroscopic plate disclosed in Patent Document 2 includes a cover glass (first plate-like portion) provided with a through-hole, a plate-like slide glass portion arranged to face the cover glass, and the slide glass portion. A slide glass (second plate-like portion) is provided on the main surface and includes a support portion that supports the cover glass and a convex portion provided at a position corresponding to the through hole. The convex portion is provided such that the tip of the convex portion protrudes from the through hole in a state where the cover glass is supported by the support portion. The cover glass is supported by the support portion, and the tip of the convex portion is fixed to the outer main surface of the cover glass, whereby the cover glass is fixed to the slide glass at a certain distance.

  Moreover, in the observation part which comprises a part of multifunctional observation plate used for the component analysis apparatus which measures a component automatically, it is set as the literature in which the part containing the said spectroscopic plate was disclosed, and patent No. 4911172. Gazette (patent document 3).

  The observation part disclosed in Patent Document 3 includes a plate-like part provided with a recess and a translucent sheet member. In the translucent sheet member, a part of the periphery of the translucent sheet member is adhered to a plate-like portion positioned around the recess so that a part of the recess is opened. When measuring the formed component automatically using the formed component analyzer, the objective lens is automatically focused on the translucent sheet corresponding to the cover glass or the plate-shaped portion corresponding to the slide glass. The objective lens is moved by a predetermined distance from the focal position, and the liquid sample is observed.

JP 2002-350739 A JP 2006-201367 A Japanese Patent No. 4911172

  When inspecting a liquid sample using an integrated spectroscopic plate in which a slide glass and a cover glass are fixed in advance, a storage formed between the slide glass and the cover glass by using a capillary phenomenon. Fill the part with a liquid sample. For this reason, the spectroscopic plate is configured such that the distance between the slide glass and the cover glass is as narrow as about 0.05 mm to 0.20 mm.

  In addition, the speculum plate is often manufactured from a transparent synthetic resin material instead of a glass material in order to form it inexpensively and efficiently. Synthetic resin materials have a higher coefficient of thermal expansion and lower rigidity compared to glass materials, so when the spectroscopic plate is manufactured using synthetic resin materials, compared to the case of using glass. Thus, the spectroscopic plate is easily deformed. In particular, depending on the environment at the time of use or storage, bending deformation is likely to occur due to heating, heating, or the like being applied from the outside or an external force being applied.

  The spectroscopic plate is often bent and deformed in the direction in which the slide glass and cover glass approach each other. When such deformation occurs, the amount of liquid sample filled between the slide glass and cover glass decreases. To do. As a result, a difference occurs in the appearance rate of the formed component contained in the liquid sample, and the measurement results vary.

  Further, when the formed component analyzer is used, when the focusing operation for focusing the objective lens on the slide glass or the cover glass is performed, the focus is shifted if the spectroscopic plate is deformed. Thereby, the inconvenience that it takes time for the focusing operation also occurs. Therefore, in order to stably measure the liquid sample, a means for suppressing the bending deformation is required.

  Here, in the spectroscopic plate disclosed in Patent Document 1, for example, a cover glass is fixed to the slide glass at a certain distance by a convex portion provided on the slide glass. Since the convex portions are provided at a considerable distance along the longitudinal direction or the short side direction of the cover glass or the slide glass, the spectroscopic plate covers the central portion located between the convex portions. It becomes easy to bend and deform in the direction in which glass and slide glass approach each other.

  In the spectroscopic plate disclosed in Patent Document 2, since the through holes are provided at the four corners of the cover glass, the convex portions inserted into the cover glass to support the cover glass are also arranged in the longitudinal direction or short side of the cover glass. They are arranged at a considerable distance along the direction. The support part provided separately from the convex part is arranged outside the convex part. For this reason, the cover glass is easily bent and deformed in the direction approaching the slide glass at the central portion located inside the convex portion.

  In the observation part disclosed in Patent Document 3, since a part of the periphery of the sheet member is fixed to the plate-like part, the sheet part is easily bent and deformed so that the center part of the sheet member approaches the plate-like member.

  As described above, in the spectroscopic plate disclosed in Patent Document 1 to Patent Document 3 and the observation unit corresponding thereto, the bending deformation cannot be suppressed, and it becomes difficult to stably measure the liquid sample. .

  The present invention has been made in view of the above problems, and an object of the present invention is to provide a spectroscopic plate capable of stably measuring a liquid sample.

The spectroscopic plate according to the present invention is an spectroscopic plate that is used for inspecting a liquid sample using a microscope and includes a reservoir for filling the liquid sample therein, and defines a bottom surface of the reservoir A glass slide part, a cover glass part disposed opposite to the glass slide part and defining a top surface of the storage part, and at least part of the periphery of the storage part, the glass slide part And a cover portion that maintains a state where the cover glass portion and the cover glass portion are opposed to each other, a window portion for supplying a liquid sample to the storage portion, and the slide glass portion and the cover that are located inside the storage portion When at least one of the glass portions is bent and deformed, the distance between the slide glass portion at the portion defining the bottom surface and the cover glass portion at the portion defining the top surface is reduced. And a suppressing spacer portion. A plurality of the spacer portions (provided that the number is three or less) are provided apart from each other inside the storage portion. The cover is separated from each other so that the storage portion is located in the state where the cover glass portion is opposed to the slide glass portion and protrudes toward the slide glass portion. A pair of engaging protrusions provided on the glass portion is included. The slide glass portion includes a pair of engagement groove portions provided to be engageable with the pair of engagement protrusions. The cover glass portion is fixed to the slide glass portion when the pair of engagement protrusions engage with the pair of engagement groove portions. The slide glass portion has a pair of end portions located outside the pair of engagement groove portions in a direction in which the pair of engagement groove portions are arranged. The end portion is provided to be lower in height than a portion that defines the bottom surface. The end portion is inclined so as to decrease in height toward the engagement groove portion, and has an inclined portion that guides the engagement protrusion portion to the engagement groove portion. The support portion further includes a peripheral wall portion surrounding the storage portion. The peripheral wall portions are positioned inside the pair of engagement projection portions and the pair of engagement groove portions in an engaged state in which the pair of engagement projection portions and the pair of engagement groove portions are engaged. However, the spectroscopic plate according to the present invention includes a substrate portion and a lid portion, and the substrate portion is provided with a concave portion for accommodating a sample, and the lid portion is connected to the substrate portion via a hinge portion. It is provided integrally and can cover the concave portion, and the lid portion is provided with a notch portion that becomes an opening for sample injection when the lid portion is covered with the concave portion. The depth of the concave portion is a depth at which capillary action occurs between the lower surface of the lid portion and the bottom surface of the concave portion with the lid portion covered on the concave portion. This is not the case for a preparation for liquid sample inspection, characterized in that a spacer for maintaining a gap between the bottom surface of the lid portion and the bottom surface of the concave portion is provided on the bottom surface of the portion and / or the bottom surface of the lid portion.

  The slide glass portion and the cover glass portion have a light-transmitting property so that the liquid sample can be sandwiched and held during microscopic observation and the liquid sample can be observed. Moreover, as long as it has translucency, a slide glass part and a cover glass part are not limited to glass, The thing comprised by transparent synthetic resin is also included.

  In the spectroscopic plate according to the present invention, the spacer portion includes a bottom surface side protruding portion that protrudes from the slide glass portion of the portion that defines the bottom surface toward the cover glass portion. It is preferable.

  In the spectroscopic plate according to the present invention, the spacer portion includes a top surface side protruding portion protruding from the cover glass portion of the portion defining the top surface toward the slide glass portion side. It is preferable that

  In the spectroscopic plate according to the present invention, the spacer portion has a spot-like shape when viewed along the direction in which the slide glass portion and the cover glass portion are arranged to face each other. It is preferable.

In the speculum plate based on SL invention, the apex portion of the spacer portion is preferably provided in contact with the slide glass unit or the cover glass portions disposed opposite.
In the spectroscopic plate based on the said invention, 0.05 mm or more and 0.2 mm or less may be sufficient as the distance between the said slide glass part and the said cover glass part.
In the spectroscopic plate according to the present invention, the window portion is configured such that the width in the short direction of the opening portion of the slide glass portion is wider than the width in the short direction of the cover glass portion. Therefore, it is preferable that the gap is formed between the cover glass portion and the opening portion of the slide glass portion in a state where the cover glass portion is fixed to the slide glass portion.
In the spectroscopic plate according to the present invention, the liquid sample is preferably urine, blood, lymph, saliva, or gastric juice.

It is a top view of the speculum plate which concerns on Embodiment 1 of this invention. It is a schematic sectional drawing in alignment with the II-II line | wire shown in FIG. It is a bottom view of the 1st plate-shaped part shown in FIG. It is a top view of the 2nd plate-shaped part shown in FIG. It is a schematic sectional drawing which shows the state before the assembly of the spectroscopic plate shown in FIG. It is a schematic sectional drawing which shows the spectroscopic plate which concerns on Embodiment 2 of this invention. It is a schematic sectional drawing which shows the spectroscopic plate which concerns on Embodiment 3 of this invention.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. In the following embodiments, the same or common parts are denoted by the same reference numerals in the drawings, and description thereof will not be repeated.

(Embodiment 1)
FIG. 1 is a plan view of the spectroscopic plate according to Embodiment 1 of the present invention. FIG. 2 is a schematic cross-sectional view taken along line II-II shown in FIG. FIG. 3 is a bottom view of the first plate-like portion shown in FIG. FIG. 4 is a plan view of the second plate-like portion shown in FIG. With reference to FIG. 1 to FIG. 4, the speculum plate 1 according to the present embodiment will be described.

  The spectroscopic plate 1 according to the present embodiment is used for inspection of a liquid sample using a microscope, and is specifically used when observing a formed component contained in the liquid sample. Here, examples of the liquid sample include digestive fluids including saliva and gastric juice, biological fluids such as blood and lymph fluid, and biological samples obtained from a living body such as urine. In particular, the liquid sample is preferably urine sediment.

  As shown in FIGS. 1 and 2, the spectroscopic plate 1 includes a first plate-like portion 10, a second plate-like portion 20, a storage portion R, and a window portion 29. The speculum plate 1 is configured by fixing the first plate-like portion 10 and the second plate-like portion 20 so as to face each other.

  The storage portion R is configured such that the first plate portion 10 and the second plate portion 20 face each other, whereby a main surface 11a of the cover glass portion 11 and a main surface 26a of the center portion 26 of the slide glass portion 21 described later. Is a space formed between the two. The window portion 29 is formed by a portion of the opening A of the second plate-like portion 20 described later not being covered with the first plate-like portion 10, and the gap between the first plate-like portion 10 and the opening A. It is.

  As a material which comprises the 1st plate-shaped part 10 and the 2nd plate-shaped part 20, a glass material, a transparent synthetic resin material, etc. are employable, for example. As the transparent synthetic resin material, for example, an acrylic resin such as polycarbonate, polypropylene, PMMA (polymethyl methacrylate), polystyrene, or the like can be used. In addition, the 1st plate-shaped part 10 and the 2nd plate-shaped part 20 may be provided with a different material.

  In addition, about FIG. 1, since the 1st plate-shaped part 10 and the 2nd plate-shaped part 20 are transparent, it has shown with the broken line that the spacer part 15 mentioned later can be seen, but about other detailed structures Is omitted.

  As shown in FIGS. 1 and 3, the first plate-like portion 10 includes a cover glass portion 11, an engagement projection portion 12, and a spacer portion 15. The first plate-like portion 10 has a substantially rectangular shape in plan view, and has a longitudinal direction and a lateral direction. The cover glass portion 11 has a flat plate shape and has a main surface 11a facing a slide glass portion 21 described later. Main surface 11a of cover glass portion 11 includes a portion that defines top surface U1 of storage portion R. The main surface 11a of the cover glass part 11 is preferably flat.

  The engagement protrusion 12 is a portion that engages with an engagement groove 22 of the second plate-like portion 20 described later. The first plate-like portion 10 is fixed to the second plate-like portion 20 by the engagement projection portion 12 engaging with the engagement groove portion 22. The engagement protrusion 12 is provided so as to protrude from the main surface 11 a of the cover glass portion 11 toward a slide glass portion 21 described later. For example, a pair of engaging protrusions 12 are provided, and the pair of engaging protrusions 12 are provided such that the spacer portion 15 is positioned therebetween. The engagement protrusion 12 is provided so as to extend along the short direction, for example.

  The engagement protrusion 12 includes a support wall portion 13 and a lock portion 14. The support wall portion 13 is erected along the normal direction of the main surface 11a of the cover glass portion 11 and has a substantially rectangular parallelepiped shape. The lock portion 14 is a convex portion that protrudes from the distal end portion of the support wall portion 13 in the longitudinal direction of the first plate-like portion 10. The lock portion 14 is fitted into a lock groove portion 24 described later, thereby preventing the first plate-like portion 10 from being detached from the second plate-like portion 20. A plurality of the lock portions 14 are provided, and are spaced apart from each other along the short direction. The lock portion 14 may be provided from one end side to the other end side in the short direction of the cover glass portion 11.

  The spacer portion 15 is provided on the main surface 11a of the cover glass portion 11 so as to be located in the storage portion R. The spacer portion 15 protrudes from the cover glass portion 11 at the portion defining the top surface U1 toward the slide glass portion 21 side. The spacer portion 15 corresponds to a top surface side protruding portion.

  A plurality of spacer portions 15 are provided in the storage portion R so as to be separated from each other. For example, the plurality of spacer portions 15 are arranged in a lattice shape. Further, the apex portion 15 a of the spacer portion 15 is preferably provided so as to be in contact with the main surface 26 a of the central portion 26 of the slide glass portion 21. Thereby, the spacer part 15 can suppress the bending deformation | transformation of the cover glass part 11 or the slide glass part 21 more reliably.

  The shape of the spacer portion 15 can be appropriately selected from a cylindrical shape, a polygonal column shape, a truncated cone shape, a hemispherical shape, and the like. In order to suppress a decrease in the amount of liquid stored in the storage part R, the spacer part 15 has a spot-like shape when viewed along the direction in which the slide glass part 21 and the cover glass part 11 are arranged to face each other. It preferably has a shape.

  As shown in FIGS. 1, 2, and 4, the second plate-like portion 20 includes a slide glass portion 21 and a peripheral wall portion 27 as a support portion. The second plate-like portion 20 has a substantially rectangular shape in plan view, and has a longitudinal direction and a lateral direction. The longitudinal direction of the second plate-shaped portion 20 is parallel to the longitudinal direction of the first plate-shaped portion 10, and the short direction of the second plate-shaped portion 20 is parallel to the short direction of the first plate-shaped portion 10. .

  The slide glass part 21 includes an engagement groove part 22, an end part 25 located at both ends in the longitudinal direction of the second plate-like part 20, a central part 26, and an opening part A.

  The engagement groove portion 22 is a portion that engages with the engagement protrusion 12 of the first plate-like portion 10. The engagement groove portion 22 is provided at a position corresponding to the engagement projection portion 12, and a pair is provided. The engagement groove portion 22 is provided between the end portion 25 and the central portion 26. The engagement groove part 22 has a groove part 23 into which the support wall part 13 is fitted, and a lock groove part 24 into which the lock part 14 is fitted.

  The central portion 26 has a main surface 26 a that faces the main surface 11 a of the slide glass portion 21. The main surface 26a of the central portion 26 includes a portion that defines the bottom surface B1 of the storage portion R. The main surface 26a of the central portion 26 is preferably flat.

  The end portion 25 is located outside the engagement groove portion 22. The end portion 25 has an inclined portion 25 a that is inclined so that the height decreases as it goes toward the engaging groove portion 22. The inclined portion 25 a guides the movement of the engaging protrusion 12 when the engaging protrusion 12 is fitted into the engaging groove 22. The end portion 25 is preferably provided so as to be lower in height than the central portion 26, but may be the same height as the central portion 26.

  The peripheral wall portion 27 is a portion that protrudes from the main surface 26 a of the central portion 26 toward the cover glass portion 11. The peripheral wall portion 27 has a frame shape and is provided so as to surround the main surface 26 a of the central portion 26. The peripheral wall portion 27 is provided so as to be disposed inside the engagement projection portion 12. The peripheral wall portion 27 contacts the main surface 11 a of the cover glass portion 11 and supports the cover glass portion 11 from the inside of the engaging projection portion 12.

  The opening A is a portion that is defined by the main surface 26a of the central portion 26 and the peripheral surface 27a of the peripheral wall portion 27 and opens toward the cover glass portion 11 side. The width W2 (see FIG. 4) of the opening A in the short direction is configured to be wider than the width W1 (see FIG. 3) of the first plate-like portion 10 in the short direction. Thereby, in the state where the first plate-like portion 10 is fixed to the second plate-like portion 20, a gap (window portion 29) is formed between the first plate-like portion 10 and the opening A. The window portion 29 communicates with the storage portion R, and at the time of inspection, the liquid sample is supplied from the window portion 29, whereby the liquid sample is filled into the storage portion R by capillary action.

  In the spectroscopic plate 1 according to the present embodiment, a spacer portion 15 is provided in the storage portion R. For this reason, for example, even when it is heated from the outside when measuring using the component analyzer, or even when an external force is applied from the outside before and during use, the cover glass part 11 and the slide The distance between the portion of the slide glass portion 21 that defines the bottom surface B1 of the storage portion R and the portion of the cover glass portion 11 that defines the top surface U1 of the storage portion R because at least one of the glass portions 21 is bent and deformed. Decrease is suppressed.

  Thereby, it is suppressed that the capacity | capacitance of the storage part R changes, and it is suppressed that the quantity of the liquid sample with which the storage part R is filled decreases. By stabilizing the amount of the liquid sample to be filled, it is possible to suppress a difference in the appearance rate of the formed component contained in the liquid sample. As a result, variations in measurement results are suppressed, and a liquid sample can be measured stably.

  In addition, by providing the peripheral wall portion 27 so as to surround the main surface 26a of the central portion 26 in the slide glass portion 21, it is possible to prevent the liquid sample from entering the engaging groove portion 22 at the time of inspection. Thereby, it can also suppress further that the quantity of a liquid sample changes at the time of inspection. On the other hand, as long as the liquid sample is filled in the storage portion R so as not to reach the engagement groove portion 22, the peripheral wall portion 27 does not necessarily have a frame shape, and may be provided in a part of the periphery of the storage portion R. Good.

  FIG. 5 is a schematic cross-sectional view showing a state before the spectroscopic plate shown in FIG. 1 is assembled. With reference to FIG. 5, the assembly method of the spectroscopic plate 1 is demonstrated.

  As shown in FIG. 5, when assembling the speculum plate 1, first, the first plate-like portion 10 and the second plate-like portion 20 having the above-described configuration are prepared, and the first plate-like portion 10 is engaged. The first plate-like portion 10 and the second plate-like portion 20 are aligned so that the joint projection portion 12 and the engagement groove portion 22 of the second plate-like portion 20 face each other. Subsequently, the first plate-like portion 10 is moved in the direction of the arrow, and the engaging protrusion 12 is fitted into the engaging groove 22. Thereby, the 1st plate-shaped part 10 is fixed to the 2nd plate-shaped part 20, and the speculum plate 1 is comprised.

(Embodiment 2)
FIG. 6 is a schematic cross-sectional view showing the spectroscopic plate according to the present embodiment. With reference to FIG. 6, the spectroscopic plate 1A according to the present embodiment will be described.

  As shown in FIG. 6, the spectroscopic plate 1A according to the present embodiment is not the first plate-like portion 10A side but the second plate-like portion 20A, as compared with the spectroscopic plate 1 according to the first embodiment. The other difference is that the spacer portion 28 is provided on the side, and the other configurations are substantially the same.

  The first plate-like portion 10A includes a cover glass portion 11 and an engagement projection portion 12. When the engagement projection portion 12 engages with the engagement groove portion 22 of the second plate-like portion 20A, the second plate It is being fixed to the shape part 20A.

  The second plate-like portion 20 </ b> A includes a slide glass portion 21, a peripheral wall portion 27, and a spacer portion 28. The spacer part 28 is provided on the main surface 26 a of the central part 26 of the slide glass part 21 so as to be located in the storage part R. The spacer portion 28 protrudes from the slide glass portion 21 that defines the bottom surface B1 of the storage portion R toward the cover glass portion 11 side. The spacer portion 28 corresponds to a bottom surface side protruding portion. The apex portion 28 a of the spacer portion 28 is preferably provided so as to be in contact with the main surface 11 a of the cover glass portion 11.

  Even in the present embodiment, the spacer portion 28 includes the slide glass portion 21 and the top surface of the portion that defines the bottom surface B1 of the storage portion R by bending and deforming at least one of the slide glass portion 21 and the cover glass portion 11. It suppresses that the distance between the cover glass part 11 of the part which prescribes | regulates U1 reduces.

  As a result, also in the spectroscopic plate 1A according to the second embodiment, a change in the capacity of the storage portion R is suppressed, and substantially the same effect as in the first embodiment is obtained.

(Embodiment 3)
FIG. 7 is a schematic cross-sectional view showing the spectroscopic plate according to the present embodiment. With reference to FIG. 7, the spectroscopic plate 1B according to the present embodiment will be described.

  As shown in FIG. 7, the spectroscopic plate 1B according to the present embodiment has both the first plate-like portion 10B and the second plate-like portion 20B when compared with the spectroscopic plate 1 according to the first embodiment. Are different in that a spacer portion is provided, and other configurations are substantially the same.

  The first plate-like portion 10B includes a cover glass portion 11, an engagement projection portion 12, and a spacer portion 15, and the engagement projection portion 12 is engaged with the engagement groove portion 22 of the second plate-like portion 20A. The second plate-like portion 20B is fixed.

  The second plate-like part 20 </ b> B has a slide glass part 21, a peripheral wall part 27, and a spacer part 28. The spacer portion 28 is provided on the main surface 26 a of the central portion 26 of the slide glass portion 21.

  The spacer portions 15 and the spacer portions 28 are provided so as to be alternately arranged in the storage portion R, for example. In addition, the spacer part 15 and the spacer part 28 are not limited to the above arrangement | positioning, It can change suitably.

  Even in the present embodiment, the spacer portion 15 and the spacer portion 28 are portions of the slide glass portion that define the bottom surface B1 of the storage portion R by bending and deforming at least one of the slide glass portion 21 and the cover glass portion 11. It is suppressed that the distance between 21 and the cover glass part 11 of the part which prescribe | regulates the top | upper surface U1 reduces.

  As a result, also in the spectroscopic plate 1B according to the third embodiment, a change in the capacity of the storage portion R is suppressed, and substantially the same effect as in the first embodiment is obtained.

  Moreover, in this Embodiment, although the case where the spacer part 15 and the spacer part 28 were provided in a different position was illustrated and demonstrated, it is not limited to this, You may provide in the respectively corresponding position. In this case, the distance between the main surface 11a of the cover glass portion 11 and the main surface 26a of the central portion 26 of the slide glass portion 21 is such that the respective vertex portions face each other in contact with each other. Spacer portions that are equal are formed.

  In Embodiments 1 to 3 described above, the case where all of the first plate-like portion 10 and the second plate-like portion 20 are made of a light-transmitting glass material or synthetic resin material has been described as an example. However, the present invention is not limited to this, and it is only necessary that at least a portion of the first plate-like portion 10 and the second plate-like portion 20 constituting the storage portion R is made of the above-described material having translucency.

  In the first to third embodiments described above, the case where the peripheral wall portion 27 is provided so as to come into contact with the main surface 11a of the cover glass portion 11 has been described as an example. It may be provided so as not to contact main surface 11a, or peripheral wall 27 may not be provided. In this case, the support wall part 13 arrange | positioned at a part of circumference | surroundings of the storage part R functions as a support part which maintains the state by which the slide glass part 21 and the cover glass part 11 were opposingly arranged.

  In Embodiment 1 to 3 mentioned above, although the spacer parts 15 and 28 illustrated and demonstrated the case where it contacts the main surface 26a of the center part 26 of the slide glass part 21, or the main surface 11a of the cover glass part 11, Not limited to this, as long as at least one of the slide glass portion 21 and the cover glass portion 11 can be prevented from bending and deforming in the direction in which the slide glass portion 21 and the cover glass portion 11 approach each other in the storage portion R. The main surface 26 a of the central portion 26 of the slide glass portion 21 or the main surface 11 a of the cover glass portion 11 may not be in contact.

  In the first to third embodiments described above, the case where a plurality of spacer portions 15 or spacer portions 28 are provided has been described as an example. However, the present invention is not limited to this, and a single spacer portion 15 or a single spacer portion 28 may be provided. Moreover, the spacer part 15 or the spacer part 28 may be provided in the observation area | region of the microscope at the time of use.

  Although the embodiments of the present invention have been described above, the embodiments disclosed herein are illustrative and non-restrictive in every respect. The scope of the present invention is defined by the terms of the claims, and includes meanings equivalent to the terms of the claims and all modifications within the scope.

  1, 1A, 1B Speculum plate, 10, 10A, 10B 1st plate-like part, 11 Cover glass part, 11a Main surface, 12 Engaging projection part, 13 Support wall part, 14 Lock part, 15 Spacer part, 15a Apex Part, 20, 20A, 20B second plate-like part, 21 slide glass part, 22 engaging groove part, 23 groove part, 24 lock groove part, 25 end part, 25a inclined part, 26 central part, 27 peripheral wall part, 27a peripheral surface, 28 spacer part, 28a vertex part, 29 window part, A opening part, B1 bottom face, R storage part, U1 top surface.

Claims (8)

A spectroscopic plate that is used for inspection of a liquid sample using a microscope and includes a reservoir for filling the liquid sample,
A slide glass portion including a portion defining the bottom surface of the storage portion;
A cover glass portion that is disposed opposite the slide glass portion and includes a portion that defines the top surface of the storage portion;
A support portion that is located at least part of the periphery of the storage portion and maintains the state in which the slide glass portion and the cover glass portion are disposed to face each other.
A window for supplying a liquid sample to the reservoir;
The cover glass of the portion that defines the bottom surface by defining at least one of the slide glass portion and the cover glass portion that is located inside the storage portion and that defines the bottom surface and the top surface. A spacer part that suppresses a decrease in the distance between the parts,
The spacer part is provided with a plurality (except for the case of three or less) spaced apart from each other inside the storage part ,
The cover is spaced apart from each other such that the storage portion is located in the state where the cover glass portion is disposed opposite to the slide glass portion and protrudes toward the slide glass portion. Including a pair of engaging projections provided on the glass part,
The slide glass portion includes a pair of engagement groove portions provided to be engageable with the pair of engagement projection portions,
The cover glass portion is fixed to the slide glass portion by engaging the pair of engagement protrusions with the pair of engagement groove portions,
The slide glass portion has a pair of end portions located outside the pair of engagement groove portions in a direction in which the pair of engagement groove portions are arranged.
The end portion is provided to be lower in height than a portion defining the bottom surface,
The end portion has an inclined portion that is inclined so as to decrease in height toward the engaging groove portion, and guides the engaging protrusion portion to the engaging groove portion,
The support part further includes a peripheral wall part surrounding the storage part,
The peripheral wall portion is located inside the pair of engagement protrusions and the pair of engagement grooves in the engaged state in which the pair of engagement protrusions and the pair of engagement grooves are engaged. Plate (however, it consists of a substrate portion and a lid portion, and the substrate portion is provided with a recessed portion for accommodating a sample, and the lid portion is provided integrally with the substrate portion via a hinge portion, The concave portion can be covered, and the lid portion is provided with a notch portion serving as an opening for sample injection when the lid portion is covered with the concave portion. Is the depth at which capillary action occurs between the lower surface of the lid and the bottom surface of the concave portion with the lid portion covered on the concave portion, and the bottom surface of the concave portion and / Or on the lower surface of the lid portion, there is a spacer for maintaining a distance between the lower surface of the lid portion and the bottom surface of the concave portion. Unless a liquid sample test preparation, characterized by being kicked).   The spectroscopic plate according to claim 1, wherein the spacer portion includes a bottom surface side protruding portion that protrudes from the slide glass portion of the portion that defines the bottom surface toward the cover glass portion.   The spectroscope according to claim 1, wherein the spacer portion includes a top surface side protruding portion that protrudes from the cover glass portion of the portion that defines the top surface toward the slide glass portion side. plate.   The inspection according to any one of claims 1 to 3, wherein the spacer portion has a spot-like shape when viewed along a direction in which the slide glass portion and the cover glass portion are arranged to face each other. Mirror plate. The spectroscopic plate according to any one of claims 1 to 4 , wherein an apex portion of the spacer portion is provided so as to be in contact with the slide glass portion or the cover glass portion arranged to face each other. The spectroscopic plate according to any one of claims 1 to 5 , wherein a distance between the slide glass portion and the cover glass portion is 0.05 mm to 0.2 mm. The window portion is configured such that the width in the short direction of the opening of the slide glass portion is wider than the width in the short direction of the cover glass portion, so that the cover glass portion is the slide glass portion. The speculum plate according to any one of claims 1 to 6 , which is a gap formed between the cover glass portion and the opening portion of the slide glass portion in a state of being fixed to. The spectroscopic plate according to any one of claims 1 to 7 , wherein the liquid sample is urine, blood, lymph, saliva, or gastric juice.

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