JP3329988B2 - Plastic slide for optical microscope - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a slide for an optical microscope. Since the slide for an optical microscope of the present invention can keep the thickness of a sample constant by a simple operation without the sample coming into contact with a human body, the slide can be safely and simply examined.

[0002]

2. Description of the Related Art Conventionally, glass bases and covers have been used for optical microscope slides.
The surface of the base and cover was almost planar because it was difficult to machine the surface of the glass. Therefore, when observing a liquid sample with an optical microscope,
The sample is dropped on the glass, and the sample is covered with a cover glass and observed. However, since the thickness of the liquid phase of the sample changes each time, a complicated operation is required to keep the thickness constant.
In addition, since the sample adhered to the periphery of the cover glass, it was difficult to avoid that the sample adhered to the fingertip. In recent years, with the development of plastics, plastic bases and covers have been made, and those in which the base and the cover are integrally formed are also commercially available. In conventional slides for optical microscopes that are integrally molded, the thickness of the liquid phase is constant, but the thickness is as large as 80 to 100 μm, which is unsuitable for high-magnification observation. However, there is a drawback in that it easily leaks and easily adheres to the hand of the microscopic examiner. Particularly in recent medical examinations, slides and harmful viruses such as hepatitis virus may be mixed in the sample.
It is an important issue to prevent the sample leaking from the glass from attaching to the examiner or other human body. In order to solve such problems, the sample holding cell is based,
There has been proposed a plastic slide for an optical microscope formed of a cover and a partition wall and having an opening for sample injection at the front of the cell and a gas flow port at the rear of the cell (see JP-A-4-214519).

[0003]

The above-mentioned plastic slide for an optical microscope can make the thickness of a sample constant, and there is no possibility that the sample touches the human body, but when a sample having a large surface tension is injected. If the injection volume of the sample or sample is less than the capacity of the cell, the cover will bend due to surface tension,
There was a problem that the microscopy might be hindered.
Accordingly, an object of the present invention is to provide a slide for an optical microscope in which the thickness of a liquid phase of a sample is kept constant irrespective of the type and amount of the sample, and the sample does not adhere to a human body during handling. is there.

[0004]

According to the present invention, there are provided the following objects: (1) A cell for holding a sample is formed by a base, a cover, a column and a partition, and the base and the cover are made of transparent plastic. The partition seals the left and right sides of the cell, and has a function to maintain a constant distance between the base and the cover together with the support.The gas injection flows through the opening for sample injection at the front of the cell and the rear of the cell. A slide for an optical microscope characterized by having a small gas flow port through which liquid does not flow, (2) a base, a cover, a support, and a partition are integrally formed of plastic, and a cell for holding a sample is formed by them. The length of the cover in the front-rear direction is shorter than the base, the left and right sides of the cell are closed by a partition between the base and the cover, and grooves are provided on the base along the front and rear edges of the cover. The gap between the front portion of the cover and the cover serves as a sample injection port, the gap between the rear portion of the base and the cover serves as a gas flow port, and a sample holding cell having a predetermined interval defined by the left and right partitions and the front and rear grooves. A slide for an optical microscope, comprising: (3) a base, a cover,
The support and the partition are integrally made of plastic, forming a cell for holding the sample.The length of the cover in the front-back direction is shorter than the base, and the left and right sides of the cell are closed by the partition between the base and the cover. The front and rear portions of the base are provided with tapered portions in which the distance between the base and the cover gradually increases toward the outside, and a groove is formed on the base along the edges of the front and rear portions of the cover outside the tapered portion. Is provided, the gap between the base and the tip of the cover serves as a gas flow port, and the left and right partition walls and the front and rear tapered portions form a sample holding cell having a predetermined interval defined by the front and rear grooves. The slide for optical microscopes, which has a sample inlet that leads to the sample, and the front and rear edges of the base are weirs, and (4) the cell-side surface of the base This is achieved by providing the slide for an optical microscope according to any one of the above (1) to (3), wherein a convex scale line is provided on the surface of the optical microscope.

In the present invention, it is necessary that the base and the cover forming the sample holding cell are made of plastic, and it is preferable that the partition walls and the columns forming the cell are also made of plastic. A wide variety of plastics are available, for example,
Thermoplastic such as acrylic resin such as polymethyl methacrylate, polystyrene resin, polycarbonate resin, polysulfone resin, polyester resin, nylon resin, ethylene acrylic resin, polyethylene resin, polypropylene resin, poly-4-methylpentene-1 resin, polyvinyl chloride resin, etc. Plastics: thermosetting plastics such as phenolic resins and furan resins, and the like, and these can be used as a suitable mixture. Some of these plastics contain a plasticizer, so the material should be selected in consideration of the properties of the sample, and when heat-sterilizing, the material should be selected in consideration of the heat resistance of the plastic. Is good. For example, when it is necessary to perform a sterilization treatment, a plastic having high heat resistance such as a polycarbonate resin, a polysulfone resin, and a poly-4-methylpentene-1 resin is preferable. The plastic used for the base and the cover needs to be transparent, and particularly when observed at a high magnification of 1000 times or more, those having high optical isotropy are preferable. In general, most thermoplastics have crystallinity and exhibit birefringence upon stretching. Therefore, care must be taken during molding to prevent the occurrence of birefringence.

In recent years, plastic lenses have been developed, plastics having high transparency and optical isotropy have been developed, and plastics whose surface has been subjected to coating treatment to further enhance light transmittance have been used. Acrylic resin is mainly used as a material of these optical plastics. These are extremely excellent as materials for the slide for the optical microscope of the present invention. Further, the base and the cover may be made of the same plastic material or different plastic materials. The materials of the partition walls and the support columns are not particularly limited. However, it is preferable that the distance between the base and the cover is easily maintained constant by using plastic and integrally forming the base and the cover. It is also possible to partition the adhesive strip or adhesive. The shape and structure of the sample inlet at the front of the cell are not particularly limited. The sample is mainly a liquid, but a wide range can be used as long as the cell can be filled.

In the present invention, it is necessary to provide at least one column inside the cell in order to prevent the cover from bending. If the number of the columns is too large, the microscopy will be hindered, and therefore it is preferable that the number be three or less. When the support is provided with one support, the support may be provided near the center of the cell.
In the case of individual pieces, near the sample injection port and near the gas flow port, 3
In the case of individual cells, the vicinity of the center of the cell, the vicinity of the sample inlet, and the vicinity of the gas flow port are preferable. The shape of the support is not particularly limited, and examples include a column, a cone, a truncated cone, and a hemisphere. The size of the strut is not particularly limited, but from the viewpoint of not obstructing the field of view during microscopy, when the strut shape is cylindrical, conical, truncated conical, hemispherical, etc., the diameter is 0.5. mm or less.

The optical microscope slide of the present invention is mainly used for microscopic examination of liquids, and is most frequently used for examination of blood, cells, urine and the like in medical institutions, such as water, physiological saline, ethylene glycol, It is often used after being diluted with a medium used in the medical field such as dimethyl sulfoxide. For this reason, a fine gas flow port through which gas flows but liquid does not flow is provided at the rear of the cell. Therefore, when the sample is injected, the air inside the cell is discharged to the outside through the gas flow port, and the inside of the cell is replaced with the sample, thereby preventing the sample from leaking to the outside. The gap between the gas flow ports, which acts between the liquid and the base and the cover, can be appropriately set according to the relationship between adhesion and cohesion based on the degree of hydrophilicity and hydrophobicity, and other physical properties such as the viscosity of the liquid. There is a case where almost no liquid leaks even when the thickness becomes 1 mm, but it is preferably 500 μm or less, and more preferably 100 μm or less. When the gap between the gas flow ports is 5 μm, usually air flows but liquid cannot flow, and the gap is
Even when the thickness becomes 10 to 15 μm, the liquid hardly flows. As the shape of the gas flow port, a slit, another groove, or a microporous structure can be adopted.

Next, the structure of the slide for an optical microscope of the present invention will be described with reference to the drawings. FIG. 1 is a plan view showing one embodiment of the slide for an optical microscope of the present invention, in which three cells are provided and one column for preventing bending is provided near the intersection of diagonal lines of each cell. . 2 is a sectional view taken along line AA in FIG. 1, and FIG. 3 is a sectional view taken along line BB in FIG. The width of the cover 2 is shorter than the front and rear widths of the base 1 (see FIG. 3), and a cell 5 (see FIG.
Is formed, into which the sample is injected. The left and right sides of the cell 5 are closed by the partition walls 3. In the cell 5 indicated by a band-like portion between the partition walls, the distance between the base 1 and the cover 2 is kept constant, and the transmitted light in this portion is observed with a microscope. The thickness of this part, that is, the thickness of the liquid phase filled in the cell 5, is observed at a high magnification of several hundred times or more.
10 to 20 μm is appropriate in consideration of the depth of focus and attenuation of transmitted light, but in the case of observing blood, urine, cells, etc. at 200 to 400 times as frequently used in medical institutions in recent years, 40 to 40 μm is required. 50
μm is appropriate. In the case of injecting a sample having a large surface tension, the cover between the partition walls was bent in the conventional optical microscope slide, which sometimes hindered the microscopic examination. However, in the optical microscope slide of the present invention, the bending was suppressed by the support 15. And uniform microscopic examination of sediment components. The size of the observation portion (cell) is appropriately selected depending on the purpose of use, but is preferably in the range of 10 mm × 10 mm to 18 mm × 18 mm. In addition, the thickness of the base and cover is closely related to the performance of the condenser and objective lens of the microscope, so JI
The thickness of the base is preferably 0.5 to 1.5 mm, and the thickness of the cover is preferably in the range of 0.06 to 0.25 mm. These are selected according to the conditions of use, and the thickness of the most frequently used cover is 0.15 to 0.18 mm in JIS NO.1-5.

A groove serving as a liquid reservoir 10 is provided on the base 1 along the front edge of the cover 2. Sample is in reservoir 1
When the sample is injected into the cell 0, the sample is instantly filled into the cell 5 by capillary action from the slit 7 between the front part of the base and the cover, and the surplus is stored in the liquid reservoir 10 at the front part of the cell. When the sample is stored in the reservoir 10, even if the sample evaporates a little, the sample is replenished from the reservoir 10 to the cell 5 by capillary action,
No air bubbles are generated inside the cell. Therefore,
The use of the slide for an optical microscope of the present invention makes it possible to prepare a sample for microscopy very easily. Note that 9
Has a narrow slit shape and has the function of the aforementioned minute gas flow port, and when the sample is injected, the internal air is released from this, but even if the sample fills the cell, or Even if the optical microscope slide is tilted, the sample hardly leaks out. According to the test results, in the case of an aqueous sample, the interval is suitably 10 to 20 μm.

By the way, the thickness of the liquid phase filled in the cell is small. In this region, the sample is protected by a strong force due to the capillary phenomenon. Therefore, in the case of a sample having a large surface tension, the cover is curved toward the base. May be. Also, when a sample less than the capacity of the cell is placed, the cover may bend toward the base due to surface tension. When observing at high magnification, in general, the force of holding the sample by capillary action (adhesion of the liquid to the base and the cover) is increased to reduce the thickness of the sample (liquid phase), and the distance between the base and the cover is increased. The force which narrows works. Due to these phenomena,
It is also conceivable that the distribution of the formed components of the sample becomes non-uniform depending on the visual field, and the diagnosis is mistaken. In the slide for the optical microscope of the present invention, since the column is provided inside the cell, the cover does not bend due to the force acting on the sample, surface tension, or the like.

For precise observation at a high magnification, a thin cover is required due to the design of the optical system of the microscope. The specific thickness of the cover varies somewhat depending on the microscope and the manufacturer, but is often specified as 0.17 mm at 1000 times. With this thickness, even a glass cover bends to some extent toward the base side and the liquid phase becomes thinner at the center of the cell, but more so with a plastic cover. According to the slide of the present invention, it is possible to prevent the cover from bending even in such a case.

When a microscope is used, the sample inside the cell and the light transmitted through the base and the cover are observed by being enlarged by the optical system of the microscope. Therefore, the base and the cover are required to have high optical isotropy and flatness. However, at present, mold manufacturing technology and plastic molding technology have been advanced, and molded products comparable to glass have been obtained.

The slide for the optical microscope of FIG. 1 is integrally formed by laminating two kinds of resin plates. As is clear from the cross-sectional views shown in FIGS. In view of the structure of the reservoir and the structure of the slit between the base and the cover, so-called integral molding cannot be performed. Therefore, after the base portion and the cover portion are separately molded, for example, the space between the partition 3 and the cover 2 or the space between the partition 3 and the base 1 is fused by ultrasonic waves or the like, or bonded with a plastic adhesive. It is necessary to make them together in such a way. In the present invention, "made in one piece" means such a structure. When actually making slides for optical microscopes, the cover is cut into large strips and the other parts (base, pillars and partition walls) are integrally molded with a mold and fused between the cover and partition walls. Wearing or gluing is most efficient. There is also a method of inserting the column between the base and the cover and then fusing or bonding both, but considering the difficulty of positioning the column or the fact that the column moves and shifts in the cell after completion, it is considered. When the base portion is formed integrally with the same material including the support, the problems of positioning and displacement can be solved. The posts and cover need not necessarily be fused or glued.

FIG. 4 is also a plan view showing an embodiment of the slide for an optical microscope of the present invention. FIGS. 5, 6 and 7 are sectional views taken along lines CC, DD and EE in FIG. 4, respectively. It is shown. The structures of the base 1, the cover 2, the partition 3, and the cell 5 shown in these figures are the same as those described with reference to FIGS. 1 to 3 above.
The description about the area of the observation part of the cell, the thickness of the base and the cover, the function of the sample being instantaneously filled in the cell by the capillary phenomenon, the operation of the slits 7 and 9, and the description of the method for preparing a slide for an optical microscope are also shown in FIGS. This is the same as the description related to FIG.

However, in the slide for the optical microscope shown in FIGS. 4 to 7, a tapered portion 8 is provided between the cell 5 and the grooves 4 and 6 so that the distance between the base 1 and the cover 2 gradually increases toward the outside. ing. Here, the shape of the tapered portion may be, for example, a trumpet shape or another shape as long as the liquid can be sucked into the center of the cell by capillary action. Slits 7 and 9 are provided between the front and rear edges of the base and the front and rear edges of the cover, respectively, so that gas flows but liquid does not flow. Further, a sample inlet 11 communicating with the groove 4 is provided. Further, when the sample leaks to the outside for some reason and adheres between the slits 7 and 9 and the weir 13, the weir 13
Do not spill outside for Therefore, if the person handling the sample holds the outside of the weir 13, the sample does not come into direct contact with the body, and it is possible to prevent infection of harmful viruses such as hepatitis by inspection at medical institutions. This is one of the advantages of the present invention. Furthermore, when the sample injection amount is large and the sample is also accumulated in the grooves 4 and 6, if only the sample injection port 11 is closed, only the slits 7 and 9 are in communication with the outside air. No liquid leaks out.

In the slide for an optical microscope of the present invention, a convex scale line can be provided on the surface of the base on the cell side. The convex cross section and the shape of the ridge line of the graduation line may have any shape. In order that the image of the graduation line can be observed sharply, a shape in which the cross section at the top is an acute angle and the height is constant, that is, a triangular pyramid shape is most preferable. Also, the scale line may be provided only on a part of the cell or on the entire surface.
In addition, the scale lines are preferably arranged in a grid (see FIG. 8) or parallel lines (see FIG. 9), but may be arranged in other shapes. In addition, when there is a possibility that the cell membrane or the like of the sample may be damaged by the convex portion having a sharp cross section at the top, it is preferable to crimp the top.

Furthermore, it is necessary to consider that the depth of focus of the microscope is very shallow. Specifically, about 5 times at 300 times
8μm, about 1.5-2.5μm at 700 times, about 0.9-1μ at 1000 times
m or lower. In other words, since only a very small area is visible, the scale line is observed as a sharp straight line only when the focus is on the top of the scale line.

Even when the shape of the scale line is a triangular pyramid as described above, when the focus is on the middle, two parallel lines are formed, and when the focus is above the top, the scale line is formed. I can't observe. Since the height of the graduation line is considered to be up to about one third of the cell thickness under normal use conditions, more than half is a region where the graduation line cannot be observed. Therefore, if a conical projection is provided on a part of the scale line as shown in FIG. 10 and its height is made to correspond to the distance between the base and the cover, the scale can be observed even when focusing on any part of the cell. . For example, when the focus is close to the base surface, the microscope view shown in FIG.
When the image is observed as shown in FIG. 11A and the focus is near the cover, the image is observed as shown in FIG. 11B. Therefore, no matter what part of the sample is observed by moving the focal point up and down, the sample and the clear scale can be observed simultaneously. Some slides for optical microscopes, which are integrally formed with conventional products, are provided with a scale outside the cell, but the scale cannot be observed at all in the field of view of the microscope. This is obvious when considering the relationship between the depth of focus and the position of the scale. In the present invention, since the scale line is provided on the surface of the base on the cell side, not only can the sample be clearly observed with the sample, but also by making the scale line into a shape as shown in FIG. The scale and the sample can be observed simultaneously.

It is not practical to provide a ridge-shaped protruding portion on the inner surface of the cell because it is very difficult and expensive for glass. Although it is relatively easy to cut a groove-shaped graduation line on glass, as described above, the graduation cannot be used effectively due to the depth of focus. But,
Once plastic is processed in that way,
It is easy to duplicate a large number of molded articles. Therefore, a slide for an optical microscope provided with a scale line having a complicated shape as shown in FIG. 10 can be said to be a structure that makes the best use of the characteristics of plastic.

The optical microscope slide of the present invention can be provided with one cell on one base, or as shown in FIGS. 1, 4, 8, 9 and 12, two or more cells. Can also be provided. In recent years, many microscopic examinations have been performed in medical institutions and the like. Therefore, if two or more cells are provided on one base, the examination efficiency can be greatly improved.

Further, in the present invention, a colored plastic can be used. Depending on the nature of the sample and the purpose of the observation, it is often necessary to observe through a filter. For example, a green filter is often used for observing cells. The use of pre-colored slides for optical microscopes eliminates the need for filters.
Although it is possible to color the base glass to have the same function as the filter, it is very expensive, so that the colored base glass is rarely used. However, coloring of plastic is very easy and inexpensive.

FIGS. 13 and 14 show a base having a convex scale, a triangular cross section, a line interval of 0.3 mm, a width of 19 μm, and a perpendicular graduation line having a height of 11 μm. FIG. 2 is a diagram showing a photograph of red blood cells 17 taken with a microscope and expanding the magnification by a factor of 2 to 200 and 320. From this, it can be recognized that both the red blood cells and the scale line can be clearly observed, and the counting of the red blood cells becomes easy. Furthermore, since the height of the graduation line is 11 μm, the graduation line can be clearly observed even when the focus is moved and observed.

[0024]

The present invention will be specifically described below with reference to examples. Note that the present invention is not limited by these examples.

EXAMPLE 1 An acrylic resin was molded to produce a part (26 mm × 76 mm) in which a base, a support and a partition were integrally molded, and this was bonded to a polycarbonate plate (18 mm × 66 mm) with an adhesive. A slide for an optical microscope was prepared in which hemispherical columns having a diameter of 0.5 mm were provided at intersections of diagonal lines of the cells. A plan view and a cross-sectional view of the prepared slide for an optical microscope are shown in FIGS.

Example 2 In the same manner as in Example 1, an acrylic resin was molded to produce a part (26 mm × 76 mm) in which a base, a support and a partition were integrally molded, and this was combined with a polycarbonate plate (18 mm × 66 mm). An optical microscope slide having a hemispherical column having a diameter of 0.5 mm provided at the intersection of diagonal lines of the cells was prepared by bonding with an adhesive.
FIGS. 4 to 7 show a plan view and a sectional view of the prepared slide for an optical microscope. The difference from the first embodiment is that the sample injection port and the gas flow port are provided with tapered portions to facilitate suction of the sample into the center of the cell.

Examples 3 and 4 In the same manner as in Example 1, an acrylic resin was molded to produce a part (26 mm × 76 mm) in which a base, a support, and a partition were integrally molded, and this was joined with a polycarbonate plate (18 mm × 66 mm). Were adhered with an adhesive to prepare a slide for an optical microscope.
FIGS. 8 (Example 3) and 9 (Example 4) show plan views of the prepared optical microscope slides, and upper and lower views of the scale lines are shown in the upper and lower views of FIG. 10, respectively. The difference from the first embodiment is that at the time of molding the base, the scale of the grid (the third embodiment) or the parallel line (the fourth embodiment) is formed on the base.

Example 5 A concave portion for forming a pillar in a molding die was provided with two recesses per cell.
A slide for an optical microscope having two columns per cell was obtained by performing the same operation as in Example 1 except that a plurality of columns were provided. The plan view and cross-sectional view are shown in FIGS.

Example 6 A concave portion for forming a pillar in a molding die was formed at 3 per cell.
An optical microscope slide having three columns per cell was obtained by performing the same operation as in Example 1 except that a plurality of columns were provided. The plan view and the cross-sectional view are shown in FIGS.

Test Example 1 An optical microscope slide (hereinafter abbreviated as slide A) in which one support was provided at the center of the cell manufactured in Example 1 and the same as Example 1 except that the slide was not provided. A slide for an optical microscope having a shape (hereinafter, abbreviated as slide B) is prepared, and a blood sample having a high surface tension is injected into each slide, and centered on points a to c in the cell shown in FIG. The number of red blood cells among the formed components in the visual field was measured, and the results are shown in Table 1. Table 1 shows each visual field (points a to c).
In Table 3, the average value of three measurements was shown.

[Table 1] As shown in Table 1, in the slide A, the cover was not curved, and the number of red blood cells among the measured components in the sample was almost constant regardless of the visual field inside the cell. However, in slide B, the cover was curved due to the surface tension of the sample, and the measured number of red blood cells in the sample varied considerably depending on the visual field inside the cell. Although the column of slide A is located at the same position as point b, the column can be clearly recognized as a column during microscopy, and may not be mistaken for a formed component (such as red blood cells or white blood cells) in the sample. Did not.

[0031]

According to the present invention, there is provided a slide for an optical microscope in which the thickness of a liquid phase of a sample is kept constant irrespective of the type and amount of the sample and the sample does not adhere to the human body during handling. Provided.

[Brief description of the drawings]

FIG. 1 is a plan view showing a slide for an optical microscope obtained in Example 1. FIG.

FIG. 2 shows A in the optical microscope slide shown in FIG.
It is sectional drawing of -A.

FIG. 3 shows B in the optical microscope slide shown in FIG.
It is sectional drawing of -B.

FIG. 4 is a plan view showing a slide for an optical microscope obtained in Example 2.

FIG. 5 shows C in the optical microscope slide shown in FIG.
It is sectional drawing of -C.

FIG. 6 is a graph showing D in the optical microscope slide shown in FIG. 4;
It is sectional drawing of -D.

FIG. 7 shows E in the slide for the optical microscope shown in FIG.
It is sectional drawing of -E.

8 is a plan view of a slide for an optical microscope in which a grid-like graduation line is provided on a cell-side surface on a base obtained in Example 3. FIG.

FIG. 9 is a plan view of a slide for an optical microscope having a scale line parallel to a cell-side surface on a base obtained in Example 4.

FIG. 10 is a front view (top) and a plan view (bottom) of a part of one scale line.

11 (a) is a view in which a portion close to a slide surface of a cell (focusing on a ridge line of a convex portion of the scale line) is observed with a microscope when the scale line of FIG. 8 has a shape as shown in FIG. (B) shows the image of the field of view near the cover.

FIG. 12 is a plan view of a slide for an optical microscope in which a number of cells are provided on one base.

FIG. 13 is a diagram of a red blood cell and a scale line that are 200 times enlarged by using a base having convex scale lines having a triangular scale line, photographing a red blood cell diluted with a physiological saline with a microscope, and expanding the red blood cell by 200 times. It is shown.

FIG. 14 is a diagram of a red blood cell and a graduation line that are 300 times magnification obtained by using a base having convex graduation lines having a triangular cross section, photographing erythrocytes diluted with a physiological saline solution under a microscope and expanding the erythrocytes by a factor of 2; It is shown.

FIG. 15 is a plan view showing a slide for an optical microscope obtained in Example 5.

16 is a cross-sectional view taken along line FF of the optical microscope slide shown in FIG.

FIG. 17 is a sectional view taken along line GG of the optical microscope slide shown in FIG.

FIG. 18 is a plan view showing a slide for an optical microscope obtained in Example 6.

19 is a cross-sectional view taken along the line HH of the optical microscope slide shown in FIG.

20 is a cross-sectional view taken along the line II of the optical microscope slide shown in FIG.

FIG. 21 is a diagram showing a mirror inspection point inside a cell in Test Example 1.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Base 2 Cover 3 Partition wall 4, 6 groove 5 Cell 7, 9 Slit between base and cover 8 Tapered portion between base and cover 10 Liquid reservoir 11 Sample inlet 12 Scale line 13 Weir 14 Sample 15 Column 16 Red blood cell

Continuation of front page (56) References JP-A-4-214519 (JP, A) JP-A-1-302313 (JP, A) JP-A-7-270687 (JP, A) JP-A-63-19532 (JP) , A) Japanese Utility Model Sho 62-102147 (JP, U) (58) Fields investigated (Int. Cl. ⁷ , DB name) G02B 21/34 G01N 1/28

Claims (4)

(57) [Claims] 1. A cell for holding a sample is formed by a base, a cover, a support and a partition, and the base and the cover are made of transparent plastic, the partition seals the left and right sides of the cell, and the base and the cover together with the support. An optical microscope having a function of maintaining a constant interval, having an opening for sample injection at the front of the cell, and a fine gas flow opening at the rear of the cell through which gas flows but liquid does not flow. For slides. 2. A base, a cover, a support, and a partition are integrally formed of plastic, and a cell for holding a sample is formed by them. The length of the cover in the front-rear direction is shorter than that of the base, and the left and right sides of the cell are bases. The cover is closed by a partition wall, a groove is provided on the base along the front and rear edges of the cover, the gap between the front of the base and the cover becomes the sample inlet, and the gap between the rear of the base and the cover. Is a gas flow port, and has a sample holding cell having a predetermined interval defined by the left and right partition walls and the front and rear grooves. 3. A base, a cover, a support, and a partition are integrally formed of plastic, and a cell for holding a sample is formed by them. The length of the cover in the front-rear direction is shorter than that of the base, and the left and right sides of the cell are bases. The space between the base and the cover is tapered so that the distance between the base and the cover gradually increases toward the outside, and the front and the rear of the cover are provided outside the tapered portion. A groove is provided on the base along the edge of the base, a gap between the base and the tip of the cover serves as a gas flow port, and a sample holding cell having a predetermined interval defined by the left and right partitions and the front and rear tapered portions. A slide for an optical microscope, wherein a slide is formed, a sample inlet is provided to the front groove, and the front and rear edges of the base are weirs. 4. The slide for an optical microscope according to claim 1, wherein a convex scale line is provided on the surface of the base on the cell side.