JP5070069B2 - Fluid handling unit and fluid handling apparatus using the same - Google Patents

Description

  The present invention relates to a fluid handling unit and a fluid handling apparatus using the fluid handling unit, and in particular, a fluid handling unit that can be used as a sample analyzer for analyzing a sample such as a functional substance represented by a biological material, and a fluid using the fluid handling unit. It relates to handling equipment.

  Conventionally, as a method for specifically detecting a biological substance such as a protein, an antigen-antibody reaction is caused using an antibody against a specific biological substance, and the reaction product is visually recognized or spectroscopically measured. Various methods for detecting biological materials are known.

  Currently, methods such as ELISA (Enzyme-Linked Immunosorbent Assay) (enzyme-linked immunosorbent assay) are widely used as a method for quantifying a reaction product due to an antigen-antibody reaction of a biological substance such as a protein. In these methods, a sample analysis device called a microwell plate, in which an array of a large number of micro-recesses, generally called microwells (hereinafter referred to as “wells”), is used, and an antibody against a specific biological material that is a target substance is used. The well is coated on the wall of the well as the trap, the target substance is captured by this trap, and the target substance is detected by measuring the reaction product of the antigen-antibody reaction between the target substance and the antibody with fluorescence or a luminescent reagent. .

In general, in a method using a microwell plate such as ELISA, a well such as a sample containing a target substance or an antibody reagent is filled in the well as a reaction solution for reaction. This reaction occurs only when the components in the liquid filled in the well move by molecular diffusion and reach the bottom surface and inner wall of the well. Therefore, when the microwell plate is allowed to stand, the theoretical reaction time depends on the diffusion time of components in the liquid filled in the well. Since the molecules in the liquid move while colliding with surrounding molecules, the diffusion speed is very slow. Room temperature) at about 0.5 to 1 × 10 ⁻⁶ cm ² / sec. Therefore, the target substance located at a position away from the bottom surface or inner wall of the well in the liquid in the well hardly reacts within a practical measurement time. In addition, in order to improve the reaction efficiency in the microwell plate, it is effective to uniformly contact the bottom surface and wall surface in the well, which is the reaction part, with the reaction solution. Therefore, a larger amount of liquid is required.

  As described above, in the conventional method using a microwell plate such as ELISA, the antigen-antibody reaction proceeds only on the wall surface of the well coated with the capture antibody, so the target substance and antibody contained in the liquid added to the well There is a problem in that the reaction efficiency is poor because the substrate or the like must float until it reacts after floating, refluxing or sinking in the well and reaching the wall surface of the well. In addition, in the microwell plate that is subdivided into a large number of wells, the amount of liquid added to each well is limited, so that there is a problem that measurement sensitivity is lowered.

  In addition, in order to improve measurement sensitivity and shorten measurement time in methods such as ELISA, the surface area of the reaction surface (capturing surface) is provided with fine irregularities on the bottom surface of the well, thereby increasing the surface area of the reaction surface. A microplate capable of increasing sensitivity has been proposed (see, for example, Patent Document 1). In addition, a microchip that can increase the surface area of the reaction surface and increase the reaction efficiency in a minute space by arranging solid fine particles (beads) as a reaction solid phase in the microchannel of the microchip has also been proposed. (For example, refer to Patent Document 2). Furthermore, a microplate that can increase the surface area of the reaction surface and save the sample by providing a small-diameter recess at the center of the bottom surface of each well has also been proposed (see, for example, Patent Document 3).

JP-A-9-159673 (paragraph numbers 0009-0010) Japanese Patent Laid-Open No. 2001-4628 (paragraph numbers 0005-0006) JP-A-9-101302 (paragraph numbers 0010-0011)

  However, although the microplate proposed in Patent Document 1 can improve the measurement sensitivity, there is a problem that the reaction efficiency cannot be improved. Moreover, since the microchip proposed in Patent Document 2 is not a microwell plate generally used for a method such as ELISA, but is a microchip having a microchannel structure, the reaction efficiency can be improved. Not suitable for measurement. Furthermore, although the microplate proposed in Patent Document 3 can increase the surface area of the reaction surface to some extent to improve the reaction efficiency and measurement sensitivity, the reaction efficiency and measurement sensitivity are not sufficiently improved.

  Therefore, in view of such a conventional problem, the present invention improves reaction efficiency and measurement sensitivity with a simple structure and reduces reaction time and measurement time when used as a sample analyzer for measuring multiple samples. An object of the present invention is to provide a fluid handling unit and a fluid handling apparatus using the fluid handling unit that can be shortened.

  Another object of the present invention is to further improve the analysis accuracy in the above-described fluid handling device or the fluid handling unit used therefor, even when the amount of reagents or specimens used in the analysis is very small.

  In order to solve the above-described problems, a fluid handling unit according to the present invention includes a container body having a bottom surface portion and a side surface portion for forming a fluid storage portion therein, and having an opening at the upper end, and A partition wall portion that is erected and partitions the fluid storage portion of the container main body into a first fluid storage chamber and a second fluid storage chamber, and the first fluid storage chamber and the second fluid that pass through the partition wall portion A communication path that communicates between the storage chambers, and the communication path cooperates with the first fluid storage chamber and the second fluid storage chamber to introduce liquid into the fluid storage section from the opening of the container body. Until the amount exceeds the predetermined amount, the liquid in the first fluid storage chamber is caused to flow into the second fluid storage chamber by capillary action and the liquid in the second fluid storage chamber flows into the first fluid storage chamber. Prevents inflow and is introduced from the opening of the container body into the fluid container. That the amount of the liquid exceeds a predetermined amount, characterized in that it allows the inflow to the first fluid housing chamber of the liquid in the second fluid housing chamber.

  In this fluid handling unit, the height of the partition wall is preferably lower than the side surface of the container body, and the communication path is formed so as to extend from the lower end to the upper end of the partition wall through the partition wall. One or more slits are preferred.

  In the above fluid handling unit, it is preferable that the first fluid storage chamber is surrounded by the second fluid storage chamber. In this case, it is preferable that the container main body has a substantially cylindrical shape, and the partition wall has a substantially cylindrical shape formed substantially coaxially with the container main body, and the container main body has a substantially cylindrical shape. It is preferable to have a large-diameter portion and a small-diameter, generally cylindrical, small-diameter portion disposed below the large-diameter portion, and a partition wall portion disposed inside the small-diameter portion. In addition, the communication path is a plurality of slits, and it is preferable that the plurality of slits are formed at regular intervals in the circumferential direction of the partition wall portion, and the upper end surface of the partition wall portion is inclined inward and downward. It is preferable.

  In the fluid handling unit, the bottom surface portion of the second fluid storage chamber is inclined downward toward the first fluid storage chamber, and the height of the lowest portion of the bottom surface portion of the second fluid storage chamber is increased. It is preferable that the height is substantially the same as the height of the first fluid storage chamber. Moreover, it is preferable that the width of the slit is wider on the first fluid storage chamber side than on the second fluid storage chamber side. Further, until the amount of liquid introduced from the opening of the container main body into the fluid storage portion exceeds a predetermined amount, most of the liquid in the first fluid storage chamber flows into the second fluid storage chamber. preferable. Furthermore, it is preferable that the fluid handling unit is integrally formed.

  Further, in the above fluid handling unit, the communication path is introduced from the opening of the container body into the fluid accommodating portion due to the difference between the capillary force acting in the first fluid accommodating chamber and the capillary force acting in the second fluid accommodating chamber. Until the amount of liquid exceeds a predetermined amount, the liquid in the first fluid storage chamber is caused to flow into the second fluid storage chamber by capillary action and the first fluid storage chamber of the liquid in the second fluid storage chamber It is preferable to prevent the inflow to the water. In this case, the capillary force working in the second fluid housing chamber is larger than the capillary force working in the first fluid housing chamber.

  The fluid handling device according to the present invention comprises a device main body and a plurality of fluid handling units arranged on the device main body, and each of these fluid handling units is the fluid handling unit described above. And In this fluid handling apparatus, it is preferable that a plurality of fluid handling units are arranged in a matrix on the apparatus body. In this case, a plurality of fluid handling units may be integrally formed with the apparatus main body. Further, the apparatus main body is composed of a frame and a plurality of supports arranged substantially parallel to each other on the frame, and a plurality of fluid handling units are arranged in a row at predetermined intervals on each of these supports. It is preferable. In this case, a plurality of fluid handling units may be integrally formed with the support.

  According to the present invention, when used as a sample analyzer for measuring multiple samples, a fluid handling unit capable of improving reaction efficiency and measurement sensitivity with a simple structure and reducing reaction time and measurement time, and A fluid handling apparatus using the same can be provided.

  Further, in the fluid handling apparatus or the fluid handling unit used therefor, even when the amount of reagents or specimens used for analysis is very small, the analysis accuracy can be further improved.

  Embodiments of a fluid handling unit and a fluid handling apparatus using the fluid handling unit according to the present invention will be described below in detail with reference to the accompanying drawings.

  1 to 9B show an embodiment of a fluid handling unit and a fluid handling apparatus according to the present invention. The fluid handling apparatus 10 of the present embodiment can be used as an apparatus for analyzing a sample containing a functional substance typified by a biological substance such as a protein, for example, and is generally used to measure multiple samples called a microwell plate. It can be used as a sample analyzer for the purpose. As shown in FIG. 1, the fluid handling device 10 includes a device main body 12 and a plurality of (96 × 8 × 12 array in this embodiment) attached to the device main body 12 in a matrix. ) Fluid handling unit 16.

  As shown in FIGS. 1 and 2, the apparatus main body 12 is formed of a resin material or glass material such as polystyrene (PS), polycarbonate (PC), or polymethyl methacrylate (PMMA), and has a central portion. A substantially rectangular through-hole 11a is formed, the thickness is about several millimeters, and the length of one side is about several centimeters to several tens of centimeters. A plurality of (in this embodiment, 12) fluid handling unit supports 13 are configured. The through hole 11a of the frame 11 may be a recess having a bottom surface. Further, as the frame body 11, for example, a frame body of a standard specification such as a frame for a microplate of SBS (Society for Biomolecular Screening) standard may be used. The fluid handling unit support 13 may be formed of a transparent material. However, when the fluid handling apparatus 10 of the present embodiment is used for fluorescence measurement, in order to suppress an increase in background during fluorescence measurement, The fluid handling unit support 13 is preferably made of a member that hardly transmits light (for example, a black member).

  As shown in FIG. 2, each of the fluid handling unit supports 13 includes a substantially rectangular parallelepiped elongated support body 13a having a length substantially equal to the width of the through hole 11a of the frame 11, and the support body 13a. It is comprised from a pair of substantially rectangular protrusion part 13b which protrudes from the longitudinal direction both ends of upper part, and extends along the upper surface of the support body main-body part 13a. As shown in FIG. 1, each support body 13 a of the fluid handling unit support 13 is inserted into the through hole 11 a of the frame 11, and the pair of upper surfaces 11 b that the protrusions 13 b extend in the longitudinal direction of the frame 11. The apparatus main body 12 is assembled by mounting the fluid handling unit supports 13 on the frame 11 so as to be substantially parallel to and adjacent to each other.

  As shown in FIGS. 3 and 4, the upper surface of each support body 13 a of the fluid handling unit support 13 has a plurality of (eight in this embodiment) abbreviations having a diameter and a depth of about several millimeters. Cylindrical recesses (hereinafter referred to as “mounting recesses”) 14 are formed in a row at predetermined intervals. In these mounting recesses 14, as shown in FIG. 5, a fluid handling unit 16 is mounted.

  6 to 9B show an enlarged view of the fluid handling unit 16 mounted in the mounting recess 14 of the fluid handling apparatus 10 of the present embodiment. 6 is a plan view of the fluid handling unit 16 mounted in the mounting recess 14 of the fluid handling apparatus 10, and FIG. 7 is a sectional view taken along line VII-VII in FIG. 8A is a plan view of the fluid handling unit 16 of the fluid handling apparatus 10 of the present embodiment, FIG. 8B is a sectional view taken along line VIIIB-VIIIB in FIG. 8A, and FIG. 8C is a sectional view taken along line VIIIC-VIIIC in FIG. 8D is a partially enlarged view of FIG. 8C. 9A and 9B are views showing a state where a small amount of liquid is introduced into the fluid handling unit 16, FIG. 9A is a plan view corresponding to FIG. 8A, and FIG. 9B is a cross-sectional view corresponding to FIG. 8B. is there.

  The fluid handling unit 16 is made of, for example, a resin material such as polystyrene (PS), polycarbonate (PC), polymethyl methacrylate (PMMA), etc. As shown in FIGS. The outer large-diameter cylindrical portion 16a, the outer small-diameter cylindrical portion 16b, and the inner cylindrical portion 16c, which have substantially the same height as the depth, are integrally formed.

  The outer large-diameter cylindrical portion 16a has a height approximately half that of the fluid handling unit 16 and is a substantially cylindrical portion having an outer diameter that is substantially the same as the inner diameter of the mounting recess 14. When inserted into the mounting recess 14 and attached to the mounting recess 14, the mounting recess 14 is fitted and fixed. The lower end portion of the outer large-diameter cylindrical portion 16a is curved toward the outer small-diameter cylindrical portion 16b and inclined inward and downward, and is integrally connected to the upper end portion of the outer small-diameter cylindrical portion 16b.

  The outer small-diameter cylindrical portion 16b is a substantially cylindrical portion having a height about half that of the fluid handling unit 16 and having an outer diameter smaller than that of the outer large-diameter cylindrical portion 16a, and is the same as the outer large-diameter cylindrical portion 16a. It extends in the axial direction. A portion inclined inward and downward is formed at the lower end of the outer small-diameter cylindrical portion 16b, and a bottom surface portion extending in a direction substantially perpendicular to the axial direction of the outer small-diameter cylindrical portion 16b from the lower end of the inner and lower inclined portion. Is provided. A concave portion 16e having a diameter substantially equal to the inner diameter of the inner cylindrical portion 16c is formed on the lower surface of the bottom surface portion of the outer small diameter cylindrical portion 16b.

  The inner cylindrical portion 16c extends upward from the upper surface of the bottom surface portion of the outer small-diameter cylindrical portion 16b in the same axial direction as the outer small-diameter cylindrical portion 16b, and the upper end has a lower height than the upper portion of the outer small-diameter cylindrical portion 16b. It is a substantially cylindrical portion having an outer diameter smaller than the inner diameter of the cylindrical portion 16b. The inner cylindrical portion 16c is formed with a plurality of (eight in the present embodiment) slits 16d extending in a substantially straight line from the lower end to the upper end and penetrating the inner cylindrical portion 16c at predetermined intervals. Has been. The widths of these slits 16d are several μm to several hundred μm, and the inner side is wider than the outer side of the inner cylindrical part 16c.

  A space serving as an injection part 26 for injecting a fluid such as a liquid sample is formed in the outer large-diameter cylindrical part 16a, and a reaction chamber is provided between the outer small-diameter cylindrical part 16b and the inner cylindrical part 16c. An outer fluid storage chamber 28 that is a substantially annular space that can be used (for example, a capacity of about 30 μL or less) is formed, and a substantially columnar space that can be used as a measurement chamber in the inner cylindrical portion 16c. An inner fluid storage chamber 30 is formed.

  When a small amount (for example, about 30 μL or less) of a liquid such as a reagent is injected from the injection unit 26, the liquid is introduced into one or both of the inner fluid storage chamber 30 and the outer fluid storage chamber 28. Is represented by Z = 2T cos θ / γ · r (θ: contact angle, T: surface tension, γ: liquid specific gravity, r: capillary radius), and is more than the capillary force acting on the liquid in the inner fluid storage chamber 30 ( Since the capillary force acting on the liquid in the outer fluid storage chamber 28 (which is narrower than the diameter of the inner fluid storage chamber 30) is larger, the liquid injected into the injection portion 26 as shown in FIGS. Most of them are drawn into the outer fluid storage chamber 28 by capillarity and are held in the outer fluid storage chamber 28 as indicated by reference numeral 32. Therefore, the width W1 of the slit 16d formed in the inner cylindrical portion 16c and the width of the substantially annular outer fluid storage chamber 28 (difference between the inner diameter of the outer small-diameter cylindrical portion 16b and the outer diameter of the inner cylindrical portion 16c) W2 are: What is necessary is just to set suitably so that most liquid inject | poured into the injection | pouring part 26 may be drawn in in the outer side fluid storage chamber 28. FIG.

  In addition, from the state where most of the liquid injected into the injection part 26 is accumulated in the outer fluid storage chamber 28, when the liquid is further injected from the injection part 26 and exceeds a predetermined amount (for example, about 30 μL), The liquid flows into the inner cylindrical portion 16c through the opening or slit 16d at the upper end of the inner cylindrical portion 16c, fills the inside of the outer fluid storage chamber 28 and the inner cylindrical portion 16c, and fills the entire fluid handling unit 16. It can be spread.

  As described above, in the fluid handling unit 16 of the present embodiment, when a small amount of liquid such as a reagent is injected from the injection unit 26, most of the liquid injected into the injection unit 26 is drawn into the outer fluid storage chamber 28. In addition, since it flows in the circumferential direction in the outer fluid storage chamber 28 and is held in the outer fluid storage chamber 28, the outer fluid storage chamber 28 is used as a reaction chamber, and the sample can be obtained with a small amount of reagent. In the case of detecting the amount of water, the height of the liquid surface can be significantly increased to increase the surface area of the reaction wall surface (the inner wall surface of the outer fluid storage chamber 28), and the distance between the specimen and the reaction wall surface can be shortened. Therefore, the reaction efficiency can be improved, the reaction time can be shortened, and the cost can be reduced by reducing the amount of reagents used.

  Further, in the fluid handling unit 16 of the present embodiment, even when a small amount of reagent is used for analysis, the reagent can be stably held in the outer fluid storage chamber 28 as a reaction chamber. Analysis accuracy can be improved. In addition, when the amount of specimens available is very small and the specimen concentration of the solution containing this specimen is very low, the specimen in the solution may reach the reaction part on the wall of the well in the conventional microwell plate. However, the fluid handling unit 16 of the present embodiment can stably introduce the specimen into the outer fluid storage chamber 28 as a reaction chamber. The analysis accuracy can be improved as compared with the conventional microwell plate.

  Further, in the fluid handling unit 16 of the present embodiment, even if the reagent is not injected along the inner wall of the injection portion 26 in order to introduce the reagent into the outer fluid storage chamber 28, the inner fluid storage chamber is formed from the injection portion 26. Since the reagent introduced into the outer fluid storage chamber 28 is drawn into the outer fluid storage chamber 28 and held in the outer fluid storage chamber 28, the reagent automatically enters the outer fluid storage chamber 28 regardless of the reagent injection position. The reagent is moved and held, and the reagent injection operation is facilitated.

  If the slit 16d is formed so that the inner side is wider than the outer side of the inner cylindrical part 16c as in the fluid handling unit 16 of the present embodiment, the liquid level in the outer fluid storage chamber 28 is substantially flat. In addition, even if a small amount of liquid such as a reagent injected from the injection portion 26 (the amount less than the capacity of the outer fluid storage chamber 28), the liquid contacts the inner wall surface of the outer fluid storage chamber 28. It is possible to suppress variation in the area to be performed between the plurality of fluid handling units 16 and between each measurement.

  Further, in the fluid handling unit 16 of the present embodiment, a sufficient amount of cleaning liquid is injected from the injection portion 26 to the inside of the fluid handling unit 16 (inside of the injection portion 26, the outer fluid storage chamber 28, and the inner fluid storage chamber 30. ), The cleaning liquid can be easily discharged, so that the cleaning property is excellent and the background during measurement can be reduced. Moreover, since the height of the upper end of the inner cylindrical portion 16c is lower than the upper end of the outer large-diameter cylindrical portion 16a, a sufficient amount of cleaning liquid is injected from the injection portion 26, and the components to be removed are lifted to pipette. Since it can be discharged, the cleaning performance is superior to the case where the height of the upper end of the inner cylindrical portion 16c is the same as the upper end of the outer large-diameter cylindrical portion 16a.

  In addition, since the fluid handling unit 16 of this Embodiment can be integrally molded by injection molding etc., it can be manufactured easily. Further, as a modification of the fluid handling device 10 of the present embodiment, a plurality of fluid handling units 16 arranged in a row at a predetermined interval on the support 13 may be integrally formed by injection molding or the like, or As shown in FIG. 12, a plurality of fluid handling units 16 arranged in a matrix on the plate-like device main body 212 may be integrally formed by injection molding or the like without providing a fluid handling unit support.

  10A and 10B show a modification of the fluid handling unit 16 of the present embodiment. Since the fluid handling unit 116 of this modification has substantially the same configuration as the fluid handling unit 16 except that the upper end surface of the inner cylindrical portion 116c is inclined inward and downward, the reference numerals of the same parts are used. 100 is added and the description is omitted. When the upper end surface of the inner cylindrical portion 116c is inclined inward and downward to form the inclined surface 116f as in this modification, the tip of the pipette tip is moved when the liquid is injected into the fluid handling unit 16 using the pipette tip. Even when it hits the upper end of the inner cylindrical portion 116c, the tip of the pipette tip is smoothly guided into the inner fluid storage chamber 130, so that it is possible to prevent the inner cylindrical portion 116c from being deformed and damaged by the collision of the pipette tip. .

  Next, an example in which the fluid handling unit is used as a sample analysis unit will be described as an example of the fluid handling unit 16 of the present embodiment.

  First, 100 μL of anti-TNF-α antibody was injected from the injection portion 26 of the fluid handling unit 16 and held at 25 ° C. for 2 hours to immobilize the capture antibody on the inner wall of the fluid handling unit 16. Thereafter, 170 μL of a cleaning liquid (PBS-0.02% Tween 20) was injected from the injection unit 26 and discharged, thereby cleaning the inside of the fluid handling unit 16.

  Next, 170 μL of a blocking solution (PBS-1% BSA) was injected from the injection unit 26 and held at 4 ° C. for 16 hours to block the inner wall of the fluid handling unit 16, and then the blocking solution was discharged.

  Next, 100 μL of TNF-α antigen was injected from the injection section 26 and held at 25 ° C. for 1 hour to perform an antigen reaction (specimen reaction). Thereafter, 170 μL of a cleaning liquid (PBS-0.02% Tween 20) was injected from the injection unit 26 and discharged, thereby cleaning the inside of the fluid handling unit 16.

  Next, 100 μL of a biotin-labeled antibody was injected from the injection section 26 and held at 25 ° C. for 1 hour to perform a detection antibody reaction. Thereafter, 170 μL of a cleaning liquid (PBS-0.02% Tween 20) was injected from the injection unit 26 and discharged, thereby cleaning the inside of the fluid handling unit 16.

  Next, 100 μL of an enzyme (HRP Peroxidase Streptavidin) was injected from the injection unit 26 and held at 25 ° C. for 20 minutes to perform an enzyme reaction. Thereafter, 170 μL of a cleaning liquid (PBS-0.02% Tween 20) was injected from the injection unit 26 and discharged, thereby cleaning the inside of the fluid handling unit 16.

  Next, after injecting 50 μL of substrate (TMB) from the injection unit 26 and holding the substrate reaction at 25 ° C. for 10 minutes, 50 μL of the reaction stop solution (1N HCl) is injected from the injection unit 26 to react. After stopping, light having a wavelength of 450 nm was applied to the longitudinal direction (vertical direction) of the inner fluid storage chamber 30 to measure the absorbance of the reaction solution in the inner fluid storage chamber 30.

  Moreover, the same measurement was performed using the substantially cylindrical well similar to the recessed part 14 for attachment of the fluid handling apparatus 10 of this Embodiment as a comparative example.

  As a result, as shown in FIG. 11, in the example using the fluid handling unit 16 of the present embodiment, the absorbance is twice or more as compared with the comparative example, and the liquid amount (similar to the comparative example) It was found that the measurement intensity can be greatly increased by the amount of the capture antibody, antigen as the specimen, detection antibody, etc., and the same measurement intensity can be obtained with a much smaller liquid volume than the comparative example. .

It is a perspective view which shows embodiment of the fluid handling apparatus by this invention. It is a perspective view which shows the frame of the apparatus main body of the fluid handling apparatus of FIG. 1, and a fluid handling unit support body. It is a top view which expands and shows the fluid handling unit support body of FIG. It is the IV-IV sectional view taken on the line of FIG. It is a perspective view which shows the state which attached the fluid handling unit to the fluid handling unit support body of FIG. It is a top view which shows the fluid handling unit attached in the recessed part for attachment of the fluid handling apparatus of FIG. It is the VII-VII sectional view taken on the line of FIG. It is a top view which shows the fluid handling unit of the fluid handling apparatus of FIG. It is the VIIIB-VIIIB sectional view taken on the line of FIG. 8A. It is the VIIIC-VIIIC sectional view taken on the line of FIG. 8B. It is a partially expanded view of FIG. 8C. It is a figure which shows the state which introduce | transduced the small amount of liquids into the fluid handling unit of this Embodiment, and is a top view corresponding to FIG. 8A. It is a figure which shows the state which introduce | transduced the small amount of liquid into the fluid handling unit of this Embodiment, and is sectional drawing corresponding to FIG. 8B. It is a top view which shows the modification of the fluid handling unit of FIG. 8A-FIG. 8D. It is the XB-XB sectional view taken on the line of FIG. 10A. It is a graph which shows the measurement result of the light absorbency of an Example and a comparative example. It is a perspective view which shows the modification of the fluid handling apparatus by this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 ... Fluid handling apparatus, 11 ... Frame body, 11a ... Through-hole, 11b ... Upper surface, 12, 212 ... Apparatus main-body part, 13 ... Fluid handling unit support body, 13a ... Support-body main body part, 13b ... Protrusion part, 14 ... Mounting recess, 16, 116 ... Fluid handling unit, 16a, 116a ... Outer large diameter cylindrical portion, 16b, 116b ... Outer small diameter cylindrical portion, 16c, 116c ... Inner cylindrical portion, 16d, 116d ... Slit, 16e, 116e ... Recessed , 26, 126 ... injection part, 28, 128 ... outer fluid storage part, 30, 130 ... inner fluid storage part, 32 ... liquid, 116f ... inclined surface

Claims (19)

A container main body having a bottom surface portion and a side surface portion for forming a fluid storage portion therein and having an opening at an upper end thereof, and a fluid storage portion of the container main body standing up from the bottom surface portion of the container main body A partition wall that partitions the fluid storage chamber and the second fluid storage chamber; and a communication passage that passes through the partition wall and communicates between the first fluid storage chamber and the second fluid storage chamber. ,
The communication path cooperates with the first fluid storage chamber and the second fluid storage chamber, and the amount of liquid introduced into the fluid storage portion from the opening of the container body exceeds a predetermined amount. Until this time, the liquid in the first fluid storage chamber is caused to flow into the second fluid storage chamber by capillary action, and the liquid in the second fluid storage chamber is prevented from flowing into the first fluid storage chamber. And allowing the liquid in the second fluid storage chamber to flow into the first fluid storage chamber when the amount of liquid introduced into the fluid storage chamber from the opening of the container body exceeds the predetermined amount. A fluid handling unit. The fluid handling unit according to claim 1, wherein a height of the partition wall is lower than a side surface of the container main body. The said communicating path is one or several slits formed so that it might penetrate the said partition wall part and might be extended from the lower end of the said partition wall part to an upper end, The Claim 1 or 2 characterized by the above-mentioned. Fluid handling unit. 4. The fluid handling unit according to claim 1, wherein the first fluid storage chamber is surrounded by the second fluid storage chamber. 5. 5. The fluid according to claim 4, wherein the container main body has a substantially cylindrical shape, and the partition wall has a substantially cylindrical shape formed substantially coaxially with the container main body. Handling unit. The container body has a substantially cylindrical large-diameter portion and a small-diameter substantially cylindrical small-diameter portion disposed below the large-diameter portion, and the partition wall portion is disposed inside the small-diameter portion. The fluid handling unit according to claim 5, wherein the fluid handling unit is provided. The said communicating path is these slits, These slits are spaced apart in the circumferential direction of the said partition wall part at a fixed space | interval, The Claim 5 or 6 characterized by the above-mentioned. Fluid handling unit. The fluid handling unit according to any one of claims 4 to 7, wherein an upper end surface of the partition wall portion is inclined inward and downward. The bottom surface portion of the second fluid storage chamber is inclined downward toward the first fluid storage chamber, and the height of the lowest portion of the bottom surface portion of the second fluid storage chamber is the first fluid storage chamber. The fluid handling unit according to claim 1, wherein the fluid handling unit has substantially the same height as the fluid storage chamber. The fluid handling according to any one of claims 1 to 9, wherein a width of the slit is wider on the first fluid storage chamber side than on the second fluid storage chamber side. unit. Most of the liquid in the first fluid storage chamber flows into the second fluid storage chamber until the amount of liquid introduced into the fluid storage portion from the opening of the container body exceeds a predetermined amount. The fluid handling unit according to claim 1, wherein: The fluid handling unit according to any one of claims 1 to 11, wherein the fluid handling unit is integrally formed. The communication path is an amount of liquid introduced from the opening of the container body into the fluid accommodating portion due to a difference between a capillary force acting in the first fluid accommodating chamber and a capillary force acting in the second fluid accommodating chamber. Until the liquid exceeds the predetermined amount, the liquid in the first fluid housing chamber is caused to flow into the second fluid housing chamber by capillary action and the liquid in the second fluid housing chamber is allowed to flow. The fluid handling unit according to claim 1, wherein the fluid handling unit prevents inflow into the fluid handling unit. 14. The fluid handling unit according to claim 13, wherein a capillary force acting in the second fluid storage chamber is larger than a capillary force acting in the first fluid storage chamber. A device main body and a plurality of fluid handling units arranged on the device main body, each of which is a fluid handling unit according to any one of claims 1 to 14. A fluid handling device. The fluid handling device according to claim 15, wherein the plurality of fluid handling units are arranged in a matrix on the device body. The fluid handling apparatus according to claim 15 or 16, wherein the plurality of fluid handling units are integrally formed with the apparatus main body. The apparatus main body comprises a frame and a plurality of supports arranged substantially parallel to each other on the frame, and the plurality of fluid handling units are arranged in a row at predetermined intervals on each of these supports. The fluid handling device according to claim 15, wherein the fluid handling device is provided. 19. The fluid handling apparatus according to claim 18, wherein the plurality of fluid handling units are integrally formed with the support.

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