JP5951273B2 - Topical agent for preventing white hair - Google Patents

Description

The present invention relates to an external preparation for preventing white hair, characterized by comprising 6-benzylaminopurine and / or Nanbang extract as an active ingredient.

Although the occurrence of gray hair itself is generally understood as one of the aging phenomena, it is said that stress is also involved as a facilitating factor.
The mechanism of white hair development involves a decrease in the ability of melanocytes to produce melanin, a decrease in the ability to supply melanin, and the movement or relocation of melanocytes in the hair follicle to the dermal papilla during hair rebirth. However, there are still many unclear points about the details of the mechanism of development, and there is currently no fundamental method for improving gray hair.

The use of a wide variety of substances has been proposed as a gray hair preventing agent, but the effect is not satisfactory, and the development of a new substance having a high effect is desired.

Specifically, in the past, so-called hair dyes that only dye hair have been widely used for the occurrence of white hair, but such white hair dye is not only a passive means for cosmetic purposes, The effects are temporary, and side effects such as contact dermatitis and hair damage are likely to occur with repeated use, and there are problems in use.

On the other hand, various attempts have been made to actively prevent and improve the generation of gray hair.
For example, black sesame, one using black pine leaves or kelp (Patent Document 1), one using western arabic leaf extract (Patent Document 2), one using a thousand extract (Patent Document 3), There are those containing a plant extract such as one using an extract of okogi (Patent Document 4) and one using an extract of tomorrow (Patent Document 5), and others include 3- (3, Those using 4-dihydroxyphenyl) -L-alanine (Patent Document 6), those using c-AMP (adenosine-3,5′-cyclic monophosphate) (Patent Document 7), those using vitamin D3 ( Patent Document 8), those using minoxidil (Patent Documents 9 and 10) and those using α-melanocyte stimulating hormone (Patent Document 11) are disclosed.

JP-A-56-87515 JP-A-56-79617 JP 59-70605 A JP 60-178805 A JP 62-77307 A JP-A-62-63509 JP 62-45527 A JP 60-174705 A JP 61-165310 A JP 61-227518 A JP-A-1-207225 JP-A-5-320028

  In recent years, it has been suggested that hydrogen peroxide in the hair follicle increases due to aging (Non-patent Document 1). For the improvement of gray hair, not only the melanin production of melanocytes is promoted but also the hair follicle. It is said that it is effective to suppress the generation of hydrogen peroxide in the inside, and the use of materials by a new mechanism of action is expected.

J.M.Wood and others The FASEB Journal 2065-2075 2009

  6-Benzylaminopurine, which is an active ingredient of the present invention, is known as a functional material for hair restorer (Patent Document 12), but its effectiveness is hardly soluble in ethanol and hardly soluble in water. There were various restrictions on the design of the solubilized preparation for exhibiting the above.

Therefore, the present invention, when applied to the scalp, activates hair root melanocytes and also recovers melanin production by using a material having a function of suppressing the generation of hydrogen peroxide, thereby preventing and improving gray hair. An object of the present invention is to provide an external preparation for preventing gray hair (Problem 1).
Another object of the present invention is to provide solvent selection conditions for a solubilized preparation when 6-benzylaminopurine is used as an active ingredient (Problem 2).
Furthermore, in order to enhance the effectiveness of the white hair prevention effect of 6-benzylaminopurine or Nanbange, to provide an external preparation for white hair prevention characterized by blending a specific material that promotes penetration into the hair root. Yes (Problem 3).

The present invention has been proposed in order to achieve the above-mentioned object, and 6-benzylaminopurine and / or Nanbange is applied as an active ingredient to Problem 1. Moreover, in order to improve the effectiveness of the said active ingredient, this invention solubilizes 6-benzylamino purine with the specific water and ethanol mixed solvent with respect to the subject 2, and scalp permeability improver with respect to the subject 3 As a specific plant extract.

That is, according to the present invention, an external preparation for preventing white hair characterized by comprising 6-benzylaminopurine and / or Nanbange extract as an active ingredient is provided.
According to the present invention, the solvent used in the external preparation is a mixed solvent of water and ethanol, and the effective concentration of 6-benzylaminopurine is 0.2 to 1.0% by weight with respect to the water and ethanol solvent ratio ( (Weight ratio) is comprised of 65:35 to 10:90, and an external preparation for preventing white hair is provided. When preparing an external preparation for non-dangerous substances, the effectiveness of 6-benzylaminopurine is provided. An external preparation for preventing white hair comprising a water / ethanol solvent ratio (weight ratio) of 55:45 to 40:60 with respect to a concentration of 0.3 to 0.5% by weight is provided.

  Further, according to the present invention, in each of the above-mentioned external preparations, as scalp permeability improver, Ivy, Iris, Axor, Ascofilum, Anoki (Guibortia sueuxii), African padauku (Pterocarpus soyuxii), Amixa, Iris, Iclinin, Isaiyobara, Izayoi Bara, Inuenju (Maackia amurensis var. Buergeri), Cani elephant (Glycyrrhiza pallidiflora), Iris Root, Ural licorice (Glycyrrhiza urarensis), Econia, Cyprus , Kugenumaran, Anzalea, Walnut, Chlorella, Mulberry Leaf, Kei Nike , Gentiana, Koju, Kakuhaku, Gooh, Koki (Pterocarpus santalinus), Kokutan, Kokoboro, Komenuka, Congo coparnoch (Guibortia demeusii), Sasanishiki, Salamander, Giant, Sizoro, G , Jifushi, shimaenju (Maackia tashiroi), shyakuka (alcohol flower), jurubaba, syrob (Caesalpinia sappan), cypress (Caesalpinia sappan), strawberry, scallop, sage , Dulbergia Kokin Kinalensis (Dalbergia cochinchinensis), Dalbergia latifolia (Dalbergia latifolia), Tanki bean, Tsuyaku, Tsukimiko, Dividivi (Caesalpinia coria), Terminaria, TV, Tonen, Tonen, Tonen , Tribulus, Natsufuji (Milletcia japonica), Gancyrrhiza (Glycyrrhiza glabra), Nanten quail, Birch, Bakugai, Bakuga, Haesonika quai (Cesalpinia globularum) Pygeum africanum, hinokithiol, Himalayan oyster mushroom, cynomolgus, sandalwood, bubiga Hayflower, Bayberry, Pepper, Vernonia amygdalina, Vernonia anselmintica, Vernonia cinerea, Vernonia nomento Lasnense (Vernonia novaboracense), Vernonia praealta (Hervaia), Machia sirensis (Mackia turmumus), Macrocystis, pinewood, Maria thistle, Mulvar kinopus, Ptermocarp ), Muninga (Pterocarpusangolensis), Murasakitia pedula, Mekabu, Meadow Foam, Mokuzoku, Mokuboui, Modama, Yaeyamashitan (Pterocarpus indicus), Yanagimoku, Yarenra rnebia spp), lavandin, rambutan, gray hair prevention external preparation, characterized in that the addition of composed plant extracts in Ryuutan and one or more selected from Lessonia is provided.

That is, according to the present invention, a novel external preparation for preventing white hair characterized by comprising 6-benzylaminopurine and / or Nanbange extract as an active ingredient is provided. Further, according to the present invention, there is provided an external preparation for preventing white hair comprising an optimum solvent ratio (weight ratio) with respect to the effective concentration of 6-benzylaminopurine for the mixed solvent of water and ethanol used in the present invention. The
Furthermore, according to this invention, the external preparation for white hair prevention which mix | blended the specific plant extract as a scalp permeability improver is provided.

The present invention will be further described below.
The first invention according to the present invention is an external preparation for preventing white hair, characterized by comprising 6-benzylaminopurine and / or Nanbange extract as an active ingredient.

6-Benzylaminopurine (molecular weight 225.26) is a crystalline white powder, also known as 6-benzyladenine, which is known as an active ingredient for hair growth. As an active ingredient for preventing white hair, Is unknown. As a result of careful examination of other functionalities as a hair application material of 6-benzylaminopurine, the applicants have found that it is suitable as an active ingredient of an external preparation for preventing white hair.
That is, 6-benzylaminopurine has an excellent melanin production promoting action, and by applying this to the scalp, it is possible to obtain a new effect of preventing the generation of gray hair by activating the melanin production of the hair follicle. The present invention has been completed by finding knowledge.

  Nambange is a grass family plant that originates from South America and has a scientific name of “Zea mays L.”. When maize seeds are harvested, female flower pillars are collected and dried. In the present invention, an extract obtained by extracting Nanbange with a hydrophilic solvent is used, and preferably water or a mixed solvent of water and ethanol. Setting conditions such as increasing the extraction time to increase the extraction efficiency of active ingredients, increasing the concentration by changing the amount of solvent, heating, pressurizing, adjusting pH, etc. Is optional. Similar to 6-benzylaminopurine, the Nanbange extract was confirmed to have a melanin production promoting action and an antioxidant action derived from hydrogen peroxide elimination.

  6-Benzylaminopurine and Nanbange extract each have a strong melanin production promoting action alone, but the combined use of these extracts synergistically improves the effect. As a possible mechanism of action, it is considered that the melanin production promoting action is effectively increased by the hydrogen peroxide generation inhibiting effect of the Nanbange extract.

  The second invention according to the present invention relates to specific solvent preparation conditions and compositions for optimal solubilized formulation design of 6-benzylaminopurine.

  6-Benzylaminopurine has the effect of preventing white hair, and its formulation amount is not particularly limited, but a transparent solubilized preparation is required to efficiently exhibit the effect. Form is preferred. For practical use, compatibility with water or ethanol is particularly important, but 6-benzylaminopurine is a material that is difficult to dissolve in both, and thus special conditions need to be set. Therefore, in the present invention, formulation under optimum conditions is indispensable, so the problem has been solved by defining the blending conditions of water and ethanol mixed solvent.

  In the present invention, 6-benzylaminopurine alone is preferably 0.2 to 1.0% by weight as a sufficient amount capable of exhibiting the effect of preventing gray hair, and is preferable in terms of pharmacological effect (drug utilization) and burden on skin application. Is 0.3 to 0.5% by weight, optimally 0.5% by weight, and a solution can be prepared with a water / ethanol mixed solvent in an optimum ratio with respect to the amount used.

  As a result of various studies, it is preferable that the solvent used for the external preparation is a mixed solvent of water and ethanol, and the effective concentration of 6-benzylaminopurine is 0.2 to 1.0% by weight with respect to water and ethanol. When the solvent ratio (weight ratio) is constituted by 65:35 to 10:90, and when it is constituted by so-called non-dangerous substances (total amount of alcohol of 60% by weight or less), the effective concentration of 6-benzylaminopurine is 0. It was found that the ratio of water and ethanol solvent (weight ratio) was preferably 55:45 to 40:60 with respect to 3 to 0.5% by weight, and the present invention was completed.

  In addition, when the Nanbange extract is used in combination with 6-benzylaminopurine, the extract from the water / ethanol solvent ratio is not greatly changed relative to the formulation prepared in the above range of water / ethanol solvent ratio. It replaces with water or ethanol, and it can mix | blend in arbitrary ratios.

  A third invention according to the present invention is the external preparation according to the first invention and the second invention described above, wherein a specific scalp penetration enhancing material is added, and the effect of preventing white hair of the external preparation is effective. It was something that could be achieved. The specific scalp penetration-enhancing material found in the present invention has the probability of reaching the hair follicle site of the active ingredient 6-benzylaminopurine or Nanbange having a white hair prevention function when the external preparation of the present invention is applied to the scalp. This enhances the time effect and makes it possible to effectively contribute to the functional characteristics thereof, resulting in a ripple effect of reducing the amount of active ingredients.

  Specific examples of the scalp penetration enhancer referred to in the present invention are listed below. That is, Ivy, Iris, Axor, Ascophyllum, Anoki (Guibortia ehie), African Padauuku (Pterocarpus soyauxii), Amica, Iris, Icrinin, Isaiyobara, Izayoibara, Inuenju (Maackiaaurur Roots, Glycyrrhiza uralensis, Echonia, Gaiyou, Kaikekenori, Pepper, Cantharides, Raspberry, Red-breasted (Tachibana), Gyokumaish, Kugenumaran, Anemone, Walnut, Chlorella, Kuwaji, Nai Kohaku, Gooh, Koki (Pte ocarpus santalinus), cocktan, cocobolo, rice bran, congo coparnoki (Guibortia demeusii), sasanishiki, salamander, diou, shiomidro, assassin, cinnamon (Glycyrrhiza echinata), cinnabar Liquor flower), Jurbeba, Sirob (Caesalpinia bonduc), Japanese cypress (Caesalpinia sappan), Strawberry, Papaver, Sekii, Sekishozu, Sekinanto, Sederwood, Sekisei, Sekizu, cogul s), Dalbergia latifolia, Dalbergia latifolia, sunflower bean, tsuyaku, kimiko, dividivi (Caesalpinia coria), Terminaria, turpentine, tenmondo, tocanin, turkey, red strawberry, red strawberry Natsufuji (Milletia japonica), licorice (Glycyrrhiza glabra), Nanten quail, birch, Bakugaishi, Bakuga, Hazuminoi cki (unknown as esoteric) Nokithiol, Himalayan oyster mushrooms, Anonymouse, Byakujutsu, Buibanga (Guibortia tessmannii), Phytocollage, Fukinotou, Huhei, Brazilian me (Caesalpinia echinata), Platymiscium trinitaris berry Vernonia amygdalina, Vernonia anthelmintica, Vernonia cinerea, Vernonia enobosa, Vernonia enobense Renonia praealta (Vernonia praealta), Hokoei, Makia chinensis, Macrocystis, pinewood, Maria thistle, Mulvar quinocalin (Pterocarpus marsopium), Manjanilla, Mizubashimu , Purple medusasan (Millettier pedula), mechab, Meadowfoam, mokzok, mokuboui, modama, yaeyamashitan (Pterocarpus indicus), willow mushroom, jarensra, life-squirt, laurophia, rattan giardin (Arnebia tandu, Arnebirium lantern, Artanbia tandu And Lessonia the like, at the time of formulation design, adding constituted plant extract in one or more selected from these. In the preparation system containing 6-benzylaminopurine, these extracts are preferably extracted with water or a mixed solvent of water and ethanol, and the extract may be in the form of a solid or liquid.

  The amount of these specific additives used cannot be limited because it varies depending on the blending amount of the active ingredient or the plant species, but the stability of the active ingredient should be maintained over time, particularly in transparent solubilized preparations. Is required for quality assurance, and is usually in the range of 0.01 to 10 (weight ratio) with respect to the active ingredient 1.

  The external preparation for preventing white hair of the present invention includes forms acceptable as a category of pharmaceuticals, quasi drugs and cosmetics, and the dosage form is not particularly limited as long as it does not impair the purpose of the present invention. Although it is not, it is particularly advantageous for designing a transparent formulation. Specific examples include water / ethanol solution preparations, gels and solubilized preparations such as essences, and aerosol propellant mixed preparations using the stock solution of the present invention regardless of whether they are liquid or not.

  It should be noted that when a desired transparent preparation is prepared in the present invention, water or alcohol is not added rapidly but gradually. Also pay attention to the solvent temperature at the time of addition so as not to destroy the stable system. For example, in any case, when the concentration of 6-benzylaminopurine dissolved in the preparation stock solution is high, sufficient precautions are required because precipitation tends to occur with time at low temperatures.

  Regarding the formulation base at the time of formulation design, any liquid and solid raw materials acceptable for hair application can be used widely. At that time, various additives such as preservatives, fragrances, stabilizers, colorants, ultraviolet absorbers / scattering agents, antioxidants, humectants, thickeners, and the like can be added as necessary.

In addition to the active ingredient of the present invention, other components usually used in the external preparation for preventing white hair can be arbitrarily blended within a range not impairing the effects of the present invention. Examples thereof include c-AMP and its derivatives, forskolin, glyceride pentadecanoate, minoxidil, bFCF and the like.

Next, although the Example (manufacture example, the test example of an effect, and the formulation example) of the external preparation for white hair prevention of this invention is given, these do not limit this invention at all.

<Production Example 1>
10 kg of water was added to 1.0 kg of dried Nanbange pulverized product, followed by extraction with heating and stirring at 121 ° C. for 15 minutes, followed by filtration to obtain an extract.

<Production Example 2>
10 kg of 50% ethanol was added to 1.0 kg of dried Nanbange pulverized product, followed by stirring and extraction at 40 ° C. for 3 hours, followed by filtration to obtain an extract.

<Production Example 3>
Ethanol was added to the Nanbange extract obtained in Production Example 1 and stirred to prepare a solvent ratio corresponding to 60% ethanol, left standing for 24 hours and then filtered to obtain a clear Nanbange extract.

<Test Example 1> Melanin production promoting action a) Test method
B16 mouse melanoma cells (F10 strain) were seeded in a 24-well plate at 0.12 × 10 ⁵ cells / well, cultured in 10% FBS-containing MEM medium, 5% CO ₂ and 37 ° C. for about 24 hours. Added. After further culturing for 3 days, the medium was removed, washed twice with PBS (−), added with 0.25 mL of 2 mol / L sodium hydroxide solution, and heated at 65 ° C. for 1 hour to lyse the cells. With respect to the cell lysate, the absorbance at 470 nm was measured using a 2 mol / L sodium hydroxide solution as a control using a microplate reader to obtain the amount of melanin produced. At the same time, the cells were cultured under the same conditions, and the number of living cells was measured by the WST-8 assay using Cell Count Reagent SF (manufactured by Nacalai Tesque), and corrected as the amount of melanin produced per cell.

b) Test results The test results are shown in Table 1.
In the Nanbange extract and 6-benzylaminopurine, melanin production promoting action was observed in a concentration-dependent manner. Further, when the Nanbange extract and 6-benzylaminopurine were used in combination, a synergistic effect was observed.

<Test Example 2> Hydrogen peroxide scavenging action a) Test method 10 μL to 240 μL (final 1 to 24%) of a sample solution was added to 10 μL of a 1.5 mmol / L hydrogen peroxide solution, and the total volume was adjusted to 1 mL with purified water. Then, it reacted at 37 degreeC for 20 minutes. Thereafter, 2 mL of coloring solution [add 1 mL of 150 units / mL peroxidase to 100 mL of 0.1 mol / L PIPES buffer (pH 7.0) containing 150 μmol / L DA-67, 0.5% Triton X-100] The mixture was added and reacted at 37 ° C. for 5 minutes. After completion of the reaction, absorbance at a wavelength of 666 nm was measured. A blank test (purified water instead of hydrogen peroxide solution) was performed and corrected in the same manner.
Hydrogen peroxide elimination rate (%) = {1− (St−Sb) / (Ct−Cb)} × 100
St: Absorbance at wavelength 666 nm of test sample solution Sb: Absorbance at wavelength 666 nm of test sample solution blank Ct: Absorbance at wavelength 666 nm of control solution Cb: Absorbance at wavelength 666 nm of control solution blank

b) Test results The test results are shown in Table 2. In the Nanbange extract of the present invention, hydrogen peroxide scavenging action was observed in a concentration-dependent manner.

<Test Example 3>
a) Test method A group of 10 black mice, and after hair removal on the back, while applying stress intermittently, the hair tonic containing the active ingredient of the present invention (Formulation Example 1) and the scalp permeability improved to the hair tonic An agent to which 0.5% of an agent (extracted with 10 weight of water relative to 1 weight of raw material) was added was prepared, and 0.1 mL was applied to the back twice a day. One month later, the regenerated hair of a certain area was collected, and the occurrence rate (%) of white hair relative to the total number of hairs was measured. The results are shown in Table 3.

b) Test results
From the results shown in Table 3, the “white hair tonic with no active ingredient” application group, which applied stress and no stress load, clearly had a higher incidence of white hair and the stress It is thought that white hair increased.
It can also be seen that in the group to which the “active ingredient” was applied, the white hair occurrence rate was significantly suppressed.
Therefore, it can be seen that the active ingredient of the present invention can inhibit the inactivation of melanocytes or activate the inactivated melanocytes, thereby preventing and improving the generation of white hair.
Furthermore, it can be seen that the effect of adding the scalp permeability improver, that is, the effective use amount of the drug is improved by adding the scalp permeability improver. This suggests that the amount of the active ingredient of the present invention can be reduced.

  Below, the formulation example of this invention is shown. In the formulation examples, “appropriate amount” means a value at which the entire formulation is 100% by weight.

<Prescription Example 1> Hair Tonic
(weight%)
A
Ethanol / water mixture (70/30) 95.0
6-Benzylaminopurine 1.0
B
Nanbange Extract (Production Example 1) 1.0
Kefiran aqueous solution 3.0

The ingredients belonging to A are uniformly stirred, and 6-benzylaminopurine is dissolved to produce a prepared stock solution. A component belonging to B, which was separately uniformly dissolved, was gradually added to the stock solution and stirred uniformly to produce a clear and stable hair tonic.

<Prescription example 2> Hair tonic
(weight%)
A
Ethanol / water mixture (55/45) 94.0
6-Benzylaminopurine 0.5
B
Nanbange Extract (Production Example 2) 2.5
Trehalose 3.0

The ingredients belonging to A are uniformly stirred, and 6-benzylaminopurine is dissolved to produce a prepared stock solution. A component belonging to B, which was separately uniformly dissolved, was gradually added to the stock solution and stirred uniformly to produce a clear and stable hair tonic.

<Prescription Example 3> Hair Tonic
(weight%)
A
Ethanol / water mixture (55 ・ 45) 94.5
Glycerin 3.0
6-Benzylaminopurine 1.0
B
Nanbange extract 1.0
(Extracted with ethanol / water 55:45)
Ivy extract 0.5
(Extracted with ethanol / water 55:45)

The ingredients belonging to A are stirred uniformly, and 6-benzylaminopurine is dissolved to produce a prepared stock solution. A component belonging to B, which was separately uniformly dissolved, was gradually added to the stock solution and stirred uniformly to produce a clear and stable hair tonic.

<Prescription Example 4> Hair Tonic
(weight%)
A
Ethanol / water mixture (60/40) 98.0
Polyethylene glycol 400 0.2
6-Benzylaminopurine 0.3
B
Nanbange Extract (Production Example 3) 1.0
Gooh extract 0.5
(Extracted with ethanol / water 60:40)

The ingredients belonging to A are stirred uniformly, and 6-benzylaminopurine is dissolved to produce a prepared stock solution. A component belonging to B, which was separately uniformly dissolved, was gradually added to the stock solution and stirred uniformly to produce a clear and stable hair tonic.

<Prescription Example 5> Hair Tonic
(weight%)
A
Ethanol / water mixture (40/60) 95.0
Polyethylene glycol 400 1.0
Diglycerin 1.0
Glycerin 0.5
6-Benzylaminopurine 0.5
B
Sodium lactate solution 1.0
Nanbange extract 1.0
(Extracted with ethanol / water 40:60)
Willow Moku Extract 1.0
(Extracted with ethanol / water 40:60)

The ingredients belonging to A are uniformly stirred, and 6-benzylaminopurine is dissolved to produce a prepared stock solution. A component belonging to B, which was separately uniformly dissolved, was gradually added to the stock solution and stirred uniformly to produce a clear and stable hair tonic.

<Prescription Example 6> Aerosol
(weight%)
A Stock solution benzyl nicotinate 0.01
Vitamin E acetate 0.05
6-Benzylaminopurine 0.5
Nanbange extract 0.5
(Extracted with ethanol / water 40:60)
Propylene glycol 2.0
Ethanol / water mixture (40/60) B
Liquefied petroleum gas (propellant) Weight ratio to stock solution A 20

The components belonging to A were uniformly mixed and dissolved and placed in an aerosol container, and B was pressurized and filled into the container by a conventional method to produce an aerosol.

<Prescription Example 7> Aerosol
(weight%)
A Stock solution benzyl nicotinate 0.01
Pentadecanoic acid glyceride 0.1
6-Benzylaminopurine 0.5
Nanbange extract 0.5
(Extracted with ethanol / water 40:60)
Rambutan extract 0.5
(Extracted with ethanol / water 40:60)
Propylene glycol 2.0
Diglycerin 1.5
Ethanol / water mixture (40/60) B
Liquefied petroleum gas (propellant) Weight ratio to stock solution A 30

The components belonging to A were uniformly mixed and dissolved and placed in an aerosol container, and B was pressurized and filled into the container by a conventional method to produce an aerosol.

It was confirmed that all of these Formulation Examples 1 to 7 had the effect of achieving the object of the present invention, as confirmed in Test Examples 1 to 3.

Claims (4)

Gray hair prevention external preparation characterized by containing, as an active ingredient, Na Nbange extract. The external preparation for preventing white hair according to claim 1, further comprising 6-benzylaminopurine as an active ingredient. The solvent used for the external preparation is a mixed solvent of water and ethanol, and the water and ethanol solvent ratio (weight ratio) is 65:35 with respect to the effective concentration of 0.2 to 1.0% by weight of 6-benzylaminopurine. The external preparation for preventing white hair according to claim 2 , wherein the external preparation is composed of 10 to 90:90. As a scalp penetration enhancer, Ivy, Iris, Axor, Ascophilum, Anoki (Guibortia ehie), African padauku (Pterocarpus soyauxii), Amyssa, Iris, Icrinin, Isaiyobara, Iayoibara, Inouebara pallidiflora), Iris root, Ural licorice (Glycyrrhiza uralensis), Echonia, Guillain, Kaikekenori, Pepper, Cantharides, Raspberry, Vulgaris (Tachibana), Gokumaishu, Kugenumai, Mitsuo, Kakure Gentian, Koju, Kohaku, Gooh, Ko Pterocarpus santalinus, cocktan, cocobolo, rice bran, Congo copalnoki (Guibortia demeusii), Sasanishiki, Salamander, Giant, Shiomidro, Shisoyo, Sinai, Glycyrrhiza cinnahu Shuyakuka (alcohol flower), jurubaba, sirob (Caesalpinia bonduc), Japanese cypress (Caesalpinia sappan), strawberry, pearl falcon, sekii, sekishozu, sekinanto, cedarwood, sakkin inensis), Dalbergia latifolia, sunflower bean, tsuyaku, moonlit, dividibi (Caesalpinia coria), Terminaria, turpentine, tenmondo, tocanin, turkey Natsuji (Milletia japonica), Antarctic licorice (Glycyrrhiza glabra), Nanten quail, Birch, Bakugaishi, Bakuga, Hasenomikai (Caesalpinia globulirum), Scots aurium num), hinokitiol, Himalayan oyster mushrooms, anemonefish, peanuts, buibuga Pepper, Vernonia amygdalina, Vernonia anthelmintica, Vernonia cinerea, Vernonia tomentosa ab, Vernonia tomentosa nse), Vernonia praealta, Hokoei, Machia chinensis, Macrocystis, pine tree, Maria thistle, Mulvar quinocari (Pterocarpus marsopium), Manjanimu (Pterocarpusangolensis), Murasakitia pedula, Mekabu, Meadowfoam, Mokuzoku, Mokuboui, Modama, Yaeyamashitan (Pterocarpus indicus), Yanagimoku, Yarensura, Boratan Preventing external preparation gray hair of claim 1 to claim 3, characterized in that the addition of composed plant extracts in Ryuutan and one selected from Lessonia or two or more.