KR101824770B1 - Anti-wrinkle cosmetic composition comprising essentially Polygonum multiflorum adventitious extract - Google Patents

Abstract

The present invention relates to a functional cosmetic composition for prevention and improvement of wrinkles of human skin containing a herbal medicine extract and a method of extracting the same, and provides a cosmetic composition for preventing and improving skin wrinkles, which comprises an active ingredient Furthermore, it exhibits excellent effects in clinical tests of antioxidation, inhibition of elastase activity, inhibition of collagenase expression and wrinkle of skin of human body, as well as having no adverse effect on the skin, and also has an excellent effect of improving skin wrinkles.

Description

An anti-wrinkle cosmetic composition comprising Polygonum multiflorum adventitious extract containing an aqueous extract of Angelica keiskei koidz.

The present invention relates to a cosmetic composition for improving skin wrinkles containing an adriamycin root extract obtained as a result of an in vitro hypotonic tissue culture as an active ingredient, and a process for producing the same.

The skin of the human body undergoes various physico-chemical changes during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. If such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging.

On the other hand, enzymes such as Matrix Metalloproteinase (MMP), which is a collagenase, are involved in aging as well as these free radicals. That is, the synthesis and degradation of extracellular matrix such as collagen in vivo is appropriately controlled. As the aging progresses, collagen synthesis decreases and promotes expression of collagen-degrading enzyme substrate metalloproteinase (MMP) And the elasticity of the skin is lowered and wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

The Polygonum multiflorum Thunberg used in the preparation of the extract of herbal medicines constituting the functional cosmetic composition for improving skin wrinkles of the present invention is a perennial herbaceous plant belonging to the genus Porphyra, and the root-cut surface is pale yellow or brown It is distributed in Jeonnam, Kyungnam North, Chungbuk, Gangwon, Gyeonggi and Hamnam provinces in Korea and it is known as one of the most famous tonic drugs in China, Japan and Korea.

Prior art related to the extract of Sasa extract is disclosed in Korean Patent Laid-Open No. 10-2009-0120810 and Korean Patent No. 10-1052189 in relation to skin whitening, and Korean Patent No. 10-0829846 is disclosed in connection with skin aging . Korean Patent No. 10-0966835 also discloses that plant mixture extracts composed of licorice, horse riding, seaweed, black shoemaker, mushroom, angelica, angelica, mulberry, white peony, gosam and gold have a whitening and anti-wrinkle effect Korean Patent No. 10-1178594 discloses a cosmetic composition having a moisturizing and antioxidative effect, which comprises an extract of Sasa extract, a jujube extract, and a extract of Hwangryeokgi. In addition, studies have been conducted on a skin wrinkle-improving functional cosmetic composition containing an enemy Sucrose extract (Patent Application No. 10-2013-0055367, functional cosmetic composition for improving skin wrinkles containing an extract of an antiseptic).

However, there have been no studies on the tissue culture of the red pepper and its products until now, and the cosmetic composition containing the extract of the adventitious roots obtained as a result of the tissue culture of red pepper or its fraction as an active ingredient has been disclosed in any of the aforementioned prior arts none.

Tissue culture of a plant refers to a series of techniques in which tissue or cells of an organ are separated from the plant, cultured aseptically under appropriate environmental conditions, and regenerated as a full-functioning plant as a plant. The advantage of tissue culture is that it can obtain a virus-free individual, isolate plants with novel characteristics that are genetically unique, propagate a large number of plants in a short time, and grow in a narrow room year-round It has the advantage of shortening the breeding period. Methods of tissue culture include complete plant culture, embryo culture, organ culture, callus culture, single cell culture, protoplast culture, and the like.

Accordingly, it is an object of the present invention to provide a cosmetic composition for improving skin wrinkles containing plant extracts of T. orientalis obtained by plant tissue culture technology and a bioreactor as an active ingredient.

Another object of the present invention is to provide various formulations using a cosmetic composition for improving skin wrinkles which is safe without skin irritation, has no side effects on the skin, and is excellent in antioxidation, inhibition of elastase activity and inhibition of collagenase expression have.

The above object of the present invention is to provide a cosmetic composition comprising, as an active ingredient, an aqueous extract of red sea bream which has a skin wrinkle-reducing effect.

(a) deriving adventitious roots from an aseptic in-cabinet induction medium from a roots of enemy sewage; (b) extracting the aqueous sodium hypochlorite obtained in the above step by adding a predetermined extraction solvent; (c) fractionating the extract obtained in the above step by adding an organic solvent for fractionation.

Herein, the induction of the coronoid root in the step (a) may be performed by a method commonly used in the art. The adventitious root induction medium can be used without any particular limitation, if there is a commonly used medium. Preferably, it is preferable to use SH (Schenk and Hildebrandt), MS (Murashige & Skoog, 1962) medium or white medium (White medium). In the present invention, plant growth regulators that can be used in conjunction with the adventitious root induction medium include indole butyric acid (IBA), indole acetic acid (IAA), naphthalene acetic acid (NAA), gibberellin (GA) or benzyl amino purine But is not limited thereto. In addition, as an attractant, a stimulus that can increase physiologically active substances may be a chemical or physical attractant, and MJ (Methyl jasmonate) and SA (salicylic acid) may be added, and the present invention is not particularly limited thereto. It will be readily appreciated by those skilled in the art that the content of the components added to the medium can be varied within a range that can achieve the effects of the present invention.

In the step (a), the adventitious roots can be inoculated at a dose of 1 to 20 g / L, preferably 5 g / L, based on living body weight.

In the step (b), the extraction solvent may be any one selected from the group consisting of purified water, a solvent selected from the group consisting of anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butyleneglycol Solvent or a combination of two or more thereof is used, and the extraction solvent added to the medicament is preferably 1 to 20 times the total volume of the medicament. The extraction step after the extraction solvent is added is carried out by a dipping method in which dipping extraction is carried out at a temperature of 5 to 37 ° C for 1 to 15 days and a dipping method in which a cooling condenser is mounted and then heated at a temperature of 50 to 95 ° C for 4 to 20 hours Or by an ultrasonic extraction method in which ultrasonic irradiation is performed for 4 to 20 hours at a temperature of 30 to 60 캜 for extraction.

The organic solvent for the fraction used in step (c) may be an organic solvent selected from ethyl acetate, diethyl ether, trichloromethane and butyl alcohol, or a mixture thereof.

On the other hand, when the extract of the present invention obtained by the method of the present invention is applied to cosmetics, the effect of improving the skin wrinkles can be exerted in an amount of 0.001 to 10% by weight based on the total weight of the cosmetic composition. When the content of the extracted active ingredient is less than 0.001% by weight based on the total weight of the cosmetic composition, the desired effect can not be sufficiently attained. Therefore, when the content of the extracted active ingredient is less than 10% , There is a problem in the stability of the cosmetic composition, which is not preferable due to the disadvantage that the appearance of the cosmetic composition and sufficient cosmetic effects can not be exhibited.

The present invention is based on the discovery that the solvent fractions, especially the ethyl acetate fraction, of the crude Sucrose root extract are safe and useful for improving skin wrinkles.

According to the present invention, there is provided a cosmetic composition comprising, as an active ingredient, an active ingredient having an antioxidative activity, an inhibitory effect on elastase activity, an inhibitory effect on collagenase expression and a skin wrinkle effect, And may be manufactured in various types of products such as skins, lotions, essences, creams, packs, foundations, and makeup bases. In terms of physical properties, it can be applied to various properties such as liquid phase, cream phase, paste phase and solid phase, and it is also apparent that the present invention can be applied to a conventional method for producing cosmetic cosmetics.

The cosmetic composition containing the extract of the present invention as an active ingredient has an excellent effect on antioxidation, inhibition of elastase activity and inhibition of collagenase expression.

Hereinafter, specific examples of examples and experimental examples will be described in order to explain the present invention in detail. However, the embodiments or experiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-mentioned examples. It is to be understood that both the foregoing description and the following examples are provided for a more complete understanding of the present invention to those skilled in the art to which the present invention pertains, It should be understood that variations can be made.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

≪ Example 1 & gt ; Induction and proliferation of adventitious roots

Sodium hypochlorite solution was surface-sterilized with 4% and 2% sodium hypochlorite solution for 20 minutes and 10 minutes, respectively, and then washed three times with sterilized water. The aseptic inflorescence was then induced using MS medium (Murashige and Skoog) supplemented with 30 g / L. Inducible plants were cut, and 3 mg / L of growth regulators were added to 3/4 MS medium containing IBA (Indole-3-butyric acid) at 22 ± 1 ℃ for 4, And cultured for 8 weeks. The medium was adjusted to pH 5.8 using 1 N potassium hydroxide (KOH) and the air was injected at 0.1 vvm and cultured in a 3 L balloon bioreactor. The rats were washed and then freeze dried and dried by hot air drying.

< Example  2> Enemy Adrenaline  Extract preparation

(1) An organic solvent extract

600 g of a 75% aqueous ethanol solution was added to 100 g of dried sodium hypochlorite, and the mixture was subjected to primary hot water extraction. The solvent was filtered and then 600 mL of an aqueous 75% ethanol solution was further added thereto. The solvent was then filtered, The secondary extracts were combined and the solvent was removed using a vacuum concentrator to obtain an ethanol extract.

(2) Ethyl acetate fraction extract

600 mL of water was added to the above Example 2- (1), and a suspension in which the ethanolic ethanol extract was evenly dispersed by ultrasonic irradiation was prepared, followed by the fractional extraction with the same amount of ethyl acetate. This is known as Whatman No. 1. 2 filter paper, concentrated using a reduced pressure concentrator, and lyophilized to obtain an ethylacetate fraction.

(3) Fractional butyl alcohol fraction extract

600 mL of water was added to the above Example 2- (1), and a suspension in which the ethanolic ethanolic extract was evenly dispersed by ultrasonic irradiation was prepared, followed by fractional extraction with the addition of the same amount of butyl alcohol. This is known as Whatman No. 1. 2 filter paper, concentrated using a vacuum concentrator, and lyophilized to obtain a butyl alcohol fraction.

< Experimental Example  1> Antioxidant effect test

In order to examine the antioxidative effect of the extracts of suppressing wrinkles and improving effects on the extracts of Sasa obtusa obtained according to Examples 2- (1), 2- (2) and 2- (3) of the present invention, free radicals Free Radical Scavenging Activity Test, and superoxide anion scavenging activity test. The free radical scavenging test was a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), and a stable free radical, DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) (Sigma) was used.

First, each 1 mL of 0.2 mM DPPH solution was diluted with methanol to a suitable concentration of each of the respective extracts of the respective extracts obtained according to the above example, and 2 mL of the solution was added. After incubation at room temperature for 10 minutes, the absorbance at 517 nm .

Meanwhile, the control group in the free radical scavenging test was set to add methanol instead of the sample solution, measure the same way, and add methanol instead of the DPPH solution to obtain the respective color correction values for the herbal medicine extract sample and the control group. Toxicol, a well-known α-tocopherol, was used (Muruhan, S. et al., In vitro antioxidant activities of solanum surattense leaf extract, Asian Pacific Journal of tropical biomedicine, 3 (1), 28-34 (2013) .

The free radical scavenging ratio was calculated numerically using the following equation (1), and it is shown in Table 2 below. In Table 1 below, the IC ₅₀ is the concentration of the sample required to achieve a 50% free radical scavenging rate, which is a value used in comparison with other constituent materials. The smaller the value, the higher the erase rate.

The active oxygen scavenging experiment was a modification of the method of Noro et al. (Chen. Pharm. Bull., 31, 3984-3987 (1983)), and the scavenging activity of superoxide anion was evaluated by xanthine / xanthine (NBT) oxidation by reactive oxygen species was measured by using an active oxygen generating system.

Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA, 0.1 mL of BSA, 0.1 mL of NBT, and 0.1 mL of the sample, and the mixture was mixed well and allowed to stand at 25 ° C for 10 minutes. Xanthine oxidase (0.1 mL) was added and reacted at 25 ° C for 20 minutes. Then, 6 mM CuCl ₂ was added to stop the reaction. Absorbance was measured at 560 nm using an ELISA reader.

Meanwhile, the control group in the active oxygen scavenging activity test was set to take purified water instead of the sample solution, measure the same method, and add purified water instead of the xanthine oxidase solution to obtain the respective color correction values for the herbal medicine extract sample and the control group. Α-tocopherol, which is well known as a common antioxidant, was used.

The active oxygen scavenging ratio was calculated numerically using the following formula (2), and the results are shown in Table 2 below. In the following Table 1, the IC ₅₀ is a concentration of the sample required to achieve 50% of the active oxygen scavenging rate, which is a numerical value used in comparison with other component materials. The smaller the value, the higher the scavenging ability.

Antioxidative Effect of the Present Sucrose Extract division Extraction and fractionation method Free radical scavenging ability
IC ₅₀ ([mu] g / mL) Active oxygen scavenging ability
IC ₅₀ ([mu] g / mL) Example 2-1 Ethanol Extract of Red Ginseng Extract 13.037 + 0.093 455.007 ± 6.422 Example 2-2 Ethyl Acetate Fraction 7.464 + 0.836 165.766 ± 6.776 Example 2-3 Enantiomeric butyl alcohol fraction 8.722 + 0.265 198.859 ± 3.077 Comparative group alpha-tocopherol 18.316 + - 7.366 222.121 + - 5.405

As shown in the above Table 1, it was confirmed that the ethylacetate fraction had a relatively high antioxidative effect, and this result was confirmed to be better than the effect of α-tocopherol, which is known as a general antioxidant.

< Experimental Example  2> Ella Stays  Active inhibition test

Elastase Inhibition Activity Test was conducted as a test for inhibiting and improving the wrinkles of the skin using the extract obtained according to each of the above Examples of the present invention as a sample.

A method for measuring the activity of Elastase, an enzyme that degrades elastin, is a method of measuring the activity of ρ-nitroaniline using N-Succinyl-Ala-Ala-Ala-Ala-Ala- And measuring absorbance at a wavelength of 410 nm to measure elasstase activity. The buffer solution was pH 8.0, 0.267 M Trizma-HCl (Sigma), the substrate solution was 8.8 mM N-Succinyl- (Ala) ₃ ρ-nitroaniline (Sigma), and the enzyme solution was 10 μg / Respectively. After mixing 20 μL of the buffer solution and 20 μL of the substrate solution and 20 μL of the enzyme solution prepared by dissolving each of the extracts according to each of the above examples into purified water to make 100 μL, the reaction was carried out in a constant temperature water bath at 25 ° C. for 15 minutes to obtain ρ-nitroaniline Is measured at a wavelength of 405 nm using an ELISA reader.

The control group for measuring the activity of the above Elastase activity was prepared by adding purified water instead of the herbal medicine extract and measuring by the same method. The case where the color correction value was obtained by adding purified water instead of the enzyme solution was set, Ursolic acid was used as an inhibitor (Lim, Suk-Won et al., The effect of two terpenoids, ursolic acid and oleanolic acid on epidermal permeability barrier and simultaneously on dermal functions, Journal of the Society of Cosmetic Scientists, 29 (2) 205-232 (2003)).

The inhibition rate of Elastase activity was calculated numerically using the following formula (3), and the results are shown in Table 2 below. In the following [Table 2], the IC ₅₀ is the concentration of the extract sample required to achieve 50% of the inhibition rate of Elastase activity, which is a value used in comparison with other ingredient materials.

The inventive Sucrose extract had an inhibitory effect on Elastase activity division Ella Stage active low performance
IC ₅₀ ([mu] g / mL) Example 2-1 63.156 ± 0677 Example 2-2 54.416 + - 0.900 Example 2-3 31.999 + 0.926 Comparative group (ursolic acid) 107.859 ± 2.113

As shown in the above Table 2, the effect of inhibiting the activity of Elastase was shown to be good in most of the Examples, and especially in the fraction of butyl alcohol, it was confirmed that the effect of inhibiting the activity of Elastase was remarkably excellent. Was found to be better than the effect of ursolic acid, which is known as a general elastase inhibitor.

< Experimental Example  3> Collagenase ( MMP -1) expression inhibitory effect test

The collagenase which is increased in expression by UV-A irradiation using the extract of the present invention obtained in accordance with each of Examples 2- (1), 2- (2) and 2- (3) MMP-1).

The normal human fibroblast (HS68, CRL 1635) used in this experiment was purchased from ATCC.

Cells were inoculated at a density of 1 × 10 ⁵ cells in DMEM (Dulbecco's Modified Eagle's Medium) supplemented with 10% bovine serum and 1% antibiotic and incubated at 37 ° C in 5% CO ₂ for 1 day. UV-A was irradiated within the range not affecting the fibroblasts cultured with the ultraviolet chamber, the medium was replaced with the medium supplemented with the sample, and the medium was cultured for 48 hours. Then, the medium was recovered and the collagenase measuring apparatus (Amersham Pharmacia The degree of production was measured by using a microscope. First, the cell culture medium was added to a 96-well plate uniformly coated with collagenase primary antibody, and antigen-antibody reaction was performed for 3 hours. After 3 hours, the second collagenase antibody conjugated with chromophore was added and reacted again for 1 hour. Then, the color development inducing substance was added to induce the coloration at room temperature for 30 minutes, and again the color development was stopped by adding 1 M sulfuric acid, and measurement was made at 450 nm using an ELISA reader. The measured values showed the degree of collagenase expression by the following formula (4), and the degree of collagenase expression in the untreated group was taken as 100, which is shown in Table 3 below. At this time, the reaction absorbance of the cell culture without treatment of the sample was used as a control, and EGCG, which is well known as a general collagenase expression inhibitor, was used as a comparative group.

Inhibition of collagenase expression by the inventive Sucrose extract division Expression level of collagenase (%) Example 2-1 60.241 + - 0.965 Example 2-2 27.108 + - 0.432 Example 2-3 57.531 + 1.358 EGCG 73.555 ± 2.867

As shown in Table 3, the collagenase inhibitory effect was good in most of the examples, and in particular, the ethyl acetate fraction was found to have a relatively excellent inhibitory effect on collagenase expression, Was found to be better than the effect of EGCG, known as a common collagenase expression inhibitor.

As a result, it is an object of the present invention to provide an antioxidant, an inhibitor of Elastase activity and an inhibitor of collagenase expression, The ethyl acetate and butyl alcohol fractions of the extracts showed better effects than α-tocopherol, ursolic acid, which is a well-known antioxidant, and EGCG, an inhibitor of collagenase expression, .

<Formulation Example  1> Formulation Example  1 and Comparative Example  One

The composition of the skin lotion wrinkle-improving lotion containing the herbal medicine extract according to Example 2- (2) was constructed as shown in Table 4 below. Here, the unit of the component content is% by weight.

Formulation Example 1 and Comparative Example 1 number ingredient Formulation Example 1 Formulation Comparative Example 1 One  Purified water Balance Balance 2  Carbomer 0.15 0.15 3  Hydroxyethyl chylcellulose 0.05 0.05 4  Butylene glycol 3.0 3.0 5  glycerin 2.0 2.0 6  Fig-1500 0.5 0.5 7  ethanol 6.0 6.0 8  Polysorbate 80 0.2 0.2 9  Triethanolamine 0.15 0.15 10  antiseptic a very small amount a very small amount 11  The herbal medicine extract according to Example 2-2 0.5 -

Of the components identified by the respective component numbers in Table 4, the components 2 and 3 were first dispersed with stirring in the component 1 (purified water), then the components 4 to 6 were added, and the components 8 to 10 were dissolved in the component 7 And component 11 were added in this order and mixed.

In Comparative Example 1 of the above-mentioned Formulation Example 1, the other components except for the herbal medicine extract according to Example 2- (2), which is the component 11, were prepared by proceeding in the same manner.

<Formulation Example  2> Formulation Example  2 and Comparative Example  2

The composition of the elastic lotion containing the herbal medicine extract according to Example 2- (2) was constructed as shown in Table 5 below. Here, the unit of the component content is% by weight.

Formulation Example 2 and Comparative Example 2 number ingredient Formulation Example 2 Formulation Comparative Example 2 One  Cetearyl alcohol 1.2 1.2 2  Glyceryl stearate 0.4 0.4 3  Polysorbate 60 0.9 0.9 4  Sorbitan sesquioleate 0.3 0.3 5  Liquid paraffin 5.0 5.0 6  Squalane 5.0 5.0 7  Maca dean nut oil 4.0 4.0 8  glycerin 4.0 4.0 9  Carbomer 0.12 0.12 10  Xanthan gum 0.03 0.03 11  antiseptic a very small amount a very small amount 12  Purified water Balance Balance 13  Arginine 0.12 0.12 14  The herbal medicine extract according to Example 2-2 1.0 -

Components 1 to 7 are first melted by heating at a temperature of 70 DEG C, and then Components 8 to 11 are dissolved and dispersed in Component 12 and emulsified by heating at 70 DEG C . Thereafter, the emulsified product was neutralized with a component 13, cooled to a temperature of 56 캜, and then added with component 14, followed by stirring and cooling to room temperature.

In Comparative Example 2 of the above-mentioned Formulation Example 2, it was set that the remaining components except for the herbal medicine extract according to Example 2- (2), which is the component 14, were produced by proceeding in the same manner.

<Formulation Example  3> Formulation Example  3 and Comparative Example  3

The composition of the elastic nutrition cream containing the herbal medicine extract according to the extraction example 2- (2) was prepared as shown in Table 6 below. Here, the unit of the component content is% by weight.

Formulation Example 3 and Comparative Example 3 number ingredient Formulation Example 3 Formulation Comparative Example 3 One  Cetearyl alcohol 1.5 1.5 2  Glyceryl stearate 1.0 1.0 3  Polysorbate 60 1.0 1.0 4  Sorbitan sesquioleate 0.3 0.3 5  Cetyl octanoate 6.0 6.0 6  Squalane 8.0 8.0 7  Apricode kernel oil 4.0 4.0 8  Dimethicone 2.0 2.0 9  glycerin 5.0 5.0 10  Magnesium aluminum silicate 0.4 0.4 11  Xanthan gum 0.03 0.03 12  antiseptic a very small amount a very small amount 13  Purified water Balance Balance 14  The herbal medicine extract according to Example 2-2 2.0 -

Of the components identified by the respective component numbers in Table 6, the components 1 to 7 are first dissolved by heating at a temperature of 70 캜, and then the components 9 to 12 are dissolved and dispersed in the component 13 and emulsified by heating at 70 캜 . Thereafter, the emulsified product was cooled to a temperature of 56 ° C, and then Component 14 was added to the resulting mixture, followed by cooling to room temperature.

In Comparative Example 3 of the above-mentioned Formulation Example 3, it was set that the other components except for the herbal medicine extract according to Example 2- (2), which is the component 14, were produced by proceeding in the same manner.

Best modes of carrying out the invention have been disclosed. Although specific terms are employed herein, they are used for the purpose of describing the invention only and are not used to limit the scope of the invention, which is defined in the claims or the claims.

< Experimental Example  4> Prevention of skin aging and evaluation of skin irritation

In order to evaluate the skin wrinkle improving effect and skin irritation of the cosmetic composition for improving skin wrinkles and aging according to the present invention, a sensory test was conducted using the elastic nutrition cream prepared in Formulation Example 3 and Comparative Example 3.

In the sensory test, the effect of improving the wrinkles of the skin was evaluated relative to the wrinkle improving effect of the elastic nutrition cream of Comparative Example 3 on the basis of the elastic nutrition cream of Formulation Example 3, Itching, burning, and erythema. The evaluation was carried out according to the blotting law standards of excellent (5 points), excellent (4 points), normal (3 points), poor (2 points) and very poor (1 point) ]. In Table 7 below, skin irritation represents a degree of no skin irritation.

Sensory evaluation and relative wrinkle-improving effect on skin irritation of Formulation Example 3 and Comparative Example 3 number Skin irritation Wrinkle improvement effect  Formulation Example 3 Comparative Formulation Example 3 One 4 4 4 2 5 5 5 3 4 4 4 4 5 5 5 5 4 4 5 6 5 4 5 7 5 5 4 8 5 5 5 9 4 5 4 10 5 5 5 11 4 4 4 12 4 4 5 13 5 5 5 14 4 4 5 15 4 4 4 16 5 5 5 17 5 4 4 18 4 5 5 19 5 4 5 20 4 4 4 Average 4.50 4.45 4.60

As shown in the above Table 7, the skin irritation evaluation score of the cosmetic composition of Formulation Example 3 according to the present invention was extremely good evaluated at 4.50 points, and skin irritation degree was low similarly to Comparative Formulation Example 3, And it was confirmed that it is excellent.

In addition, the comparative skin wrinkle improving effect of the cosmetic composition of Formulation Example 3 versus Comparative Formulation Example 3 was 4.60 points, which was found to be excellent.

< Experimental Example  5> Safety test of skin

The skin safety of Formulation Example 3, which is a cosmetic composition comprising the extract of the present invention of Example 2- (2) of the present invention and the above-mentioned extract of the present invention, i.e., skin reaction, To assess the occurrence of skin lesions, we conducted a human skin patch test according to the Korea Food & Drug Administration 's Criteria for Skin Safety Test.

Thirty healthy male and female subjects aged 20 to 50 years old were selected as test subjects. Patch tests were performed on the inside of the upper abdomen of the subjects and after 48 hours, the epidermal patches were removed. The first reading was performed after about 60 minutes of stabilization, and the second reading was performed after 96 hours of attachment. The criteria are shown in Table 8 below.

Criteria for the intelligence test reaction weight Criteria for judgment - 0 No reaction 0.5 Faint reaction + One The border is clear but weak, erythema, edema and papules ++ 2 Marked erythema, papule and blister +++ 3 Algebra

The skin response to the sample was calculated using the formula (5) as the result of reading at 48 hours and 96 hours after attaching the patch.

The skin irritation of the samples showing the positive reaction calculated based on the test results of the patch test is shown in Table 9 below.

The skin irritation degree of the positive reaction sample based on the test result of the patch test sample 48 hours 96 hours Response (%) + ++ + ++ 48 hours 96 hours Average Example 2- (2)
(1% Propanediol) - - - - 0.00 0.00 0.00 Formulation Example 3 - - - - 0.00 0.00 0.00

Determination of average skin response Average skin response Criteria 0.0 0.9 No stimulation 1.0 2.9 Light stimulus 3.0 4.9 Moderate irritation 5.0 or higher River stimulation

As shown in the above Table 9, in the human skin test of Example 2, which is the cosmetic composition comprising Example 2 (2) of the present invention and Comparative Example 2 (2) of the present invention, According to the skin response criteria shown in [10], it could be used for sensitive skin with no stimulation. Therefore, the herbal medicine extract of the present invention and the cosmetic composition containing the herb extract are safe ingredients without skin side effects.

INDUSTRIAL APPLICABILITY As described above, the present invention is an extremely useful invention in the cosmetics industry because it has an excellent effect of providing a skin wrinkle-improving functional cosmetic composition containing an effective extract of an algae root extract.

Claims (6)

delete delete delete delete Ethyl acetate extracts from Polygonum multiflorum Thunberg root were obtained by tissue culture and the supernatant was added to the extract. The supernatant was further extracted with the same volume of ethyl acetate or butyl alcohol, filtered, and concentrated under reduced pressure. A functional cosmetic composition for improving skin wrinkles containing an extract of Hasegyo corallus root as an active ingredient A cosmetic composition for the treatment of wrinkles for improving skin wrinkles characterized in that the cosmetic composition of claim 5 has any one of formulations selected from skins, lotions, essences, creams, packs, foundations and makeup bases