JP2008184441A - Skin preparation for cell growth promotion - Google Patents

Abstract

Disclosed is a skin external preparation for promoting fibroblast proliferation, which promotes dermal metabolism by an excellent fibroblast proliferation promoting action and exhibits a rough skin prevention effect and an anti-aging effect.
A skin external preparation for promoting fibroblast proliferation contains one or more plant extracts selected from lentil, cypress, camellia and holy basil.
[Selection figure] None

Description

  The present invention relates to a fiber containing one or more plant extracts selected from lentil (Lens esculenta), cypress (Piper longum), centella asiatica, and holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album). The present invention relates to a skin external preparation and cosmetic for promoting blast proliferation, and a cosmetic method using the cosmetic.

  Conventionally, women's interest in keeping the skin beautiful and healthy is very high, and changes in facial skin due to aging, especially the occurrence of wrinkles, sagging, and spots, have become serious skin problems for women. Of these, wrinkles and sagging are caused by dermal fibroblast function decline due to aging, etc., and accompanying reduction or degeneration of the dermal matrix such as collagen and elastin, as well as environmental stress such as UV and dryness, active oxygen, etc. It is thought to be caused by oxidative damage. In the field of topical skin preparations, various cell activators, antioxidants, collagen production promoters, and elastase activity inhibitors have been studied in order to prevent or improve skin aging.

  For example, as an active ingredient of a fibroblast growth promoter, a chlorella extract (Patent Document 1), a hibiscus extract (Patent Document 2), an aqueous extract of chlorella and an extract of Aloe Vera (Patent Document 3), sesame, and sanyak It is known to use Pepper, Pepper, Toki, Dokudami, Bakumondo (Patent Document 4), Phytoglycogen (Patent Document 5) and the like.

JP-A-9-40523 Japanese Patent Laid-Open No. 9-295928 Japanese Patent Laid-Open No. 10-36283 Japanese Patent Laid-Open No. 10-45615 JP 11-255657 A

  However, the effect of promoting the growth of fibroblasts of a topical skin preparation containing the above active ingredient is not always sufficient, and the development of a more effective active ingredient has been demanded.

  The present invention has been made in view of the above circumstances, found an active ingredient having an excellent fibroblast proliferation promoting effect, and provides a skin external preparation and a cosmetic excellent in anti-aging effect and rough skin prevention effect, Furthermore, it aims at providing the beauty method using this cosmetics.

  In view of the present situation, the present inventors have extensively searched for plant-derived components. Each extract of album) was unexpectedly found to have a fibroblast proliferation promoting effect, and the present invention was completed based on this finding.

In other words, the present invention relates to lentil (Lens esculenta), Japanese persimmon (Piper longum), camellia (
Centella asiatica), holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album) selected from one or more plant extracts as active ingredients, and a skin external preparation for promoting fibroblast proliferation, A cosmetic for promoting fibroblast proliferation and a cosmetic method for applying this cosmetic to the skin are provided.

  The skin external preparation for promoting fibroblast proliferation and the cosmetic for promoting fibroblast proliferation of the present invention are plant-derived components with high safety for skin, such as lentil (Lens esculenta), cypress (Piper longum), and centella (Centella), respectively. asiatica), holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album) selected from one or more plant extracts as active ingredients for promoting fibroblast proliferation, so it is excellent when applied to the skin Demonstrate the effect of promoting fibroblast proliferation. Further, since the cosmetic method of the present invention uses the cosmetic preparation for promoting the proliferation of fibroblasts of the present invention, it is possible to promote the metabolism of the dermis and obtain an excellent effect of preventing rough skin and preventing aging.

  The present invention uses, as an active ingredient, one or more plant extracts selected from lentil (Lens esculenta), cypress (Piper longum), camellia (Centella asiatica), and holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album). A skin external preparation for promoting fibroblast proliferation, a cosmetic for promoting fibroblast proliferation, and a cosmetic method for applying this cosmetic to the skin.

  The lentil (Lens esculenta) used in the present invention is a plant belonging to the genus Lens (Lens), which is mainly produced in India and Pakistan, and is known as a highly nutritious food. Hiper (Piper longum) is a plant belonging to the genus Pepperaceae that is widely distributed in Indonesia, Java, Malay and the like. Centella asiatica is a plant belonging to the genus Ceramaceae widely distributed in the tropics. Holy Basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album) is a plant belonging to the genus Ocimum, and is also called Holy Basil, Sacred Basil, etc. in English and is an annual plant native to India and Europe. These plants are used alone or in combination of a plurality of species. In addition, the production center of these plants used by this invention is not specifically limited.

  The lentil, hibachi, camellia, or holy basil used in the present invention is used as a health food or the like, but it is not known at all that these extracts have a fibroblast proliferation promoting action.

  As an extract of each plant used in the present invention, a mixture of a part or a plurality of parts such as leaves, stems, stems, bark, buds, flowers, fruits, seeds, roots or the like of each plant body or whole plant Extracts can be used, but preferably, seeds are used for lentil, fruits are used for chickweed, whole plants are used for camellia, and leaves are used for holy basil. Used.

  For extraction, each part of the plant body may be used as it is, but after appropriately performing pretreatment such as drying, chopping, crushing, pressing or fermentation, using a solvent that is appropriately low or warm. It is preferable to extract. The extraction method is not particularly limited, and examples thereof include a method of immersing one part or two or more parts of the plant in a solvent at a low temperature to a warm temperature. The extraction time is set according to the type of extraction solvent and the extraction temperature, but is preferably about 1 to 2 weeks.

  Examples of the extraction solvent include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, dipropylene glycol). 1,3-butylene glycol etc.), lower esters (ethyl acetate, butyl acetate etc.), hydrocarbons (benzene, hexane, pentane etc.), ketones (acetone, methyl ethyl ketone etc.), ethers (diethyl ether, tetrahydrofuran, di Propyl ether), acetonitrile, etc., and one or more of them can be used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Among these, it is preferable to use a lower alcohol.

  In addition, the extract of the active ingredient of each plant may be further subjected to purification treatment such as deodorization and decolorization as long as it does not affect the effects of the present invention. Furthermore, if necessary, antiseptic / antifungal agents (phenoxyethanol, paraoxybenzoates, etc.) may be added.

  As an example of a preferable extraction method for each plant, water-containing ethyl alcohol or 1,3-butylene glycol having a concentration of 0 to 100 vol% is used, and the mixture is allowed to stand at room temperature or 30 to 45 ° C and left for 1 to 10 days There is a method of filtering after extraction and allowing the filtrate obtained to stand for another week for aging, followed by filtration again.

  The total content of Japanese lentil extract, Japanese chickweed extract, camellia extract and holy basil extract in the skin external preparation for promoting fibroblast proliferation or the cosmetic for promoting fibroblast proliferation is preferably 0. It is 00001-10 mass%, More preferably, it is 0.00001-2 mass%. If the total content of one kind or two or more kinds of lentil extract, cypress extract, camellia extract, and holy basil extract is within this range, the plant extract can be stably added to the skin. High safety and high medicinal or cosmetic effects can be exhibited.

  In the present invention, the skin external preparation for promoting fibroblast proliferation or the cosmetic preparation for promoting fibroblast proliferation includes various commonly used drugs such as whitening agents, antioxidants, anti-inflammatory agents, cell activation agents. It can be used in combination with one or more medicinal agents selected from agents, ultraviolet inhibitors, humectants, blood circulation promoters and the like. Thereby, the effect of the present invention can be further enhanced.

  The skin external preparation for fibroblast growth promoter or the cosmetic preparation for promoting fibroblast proliferation of the present invention is usually applied to an external preparation or cosmetic within the range not impairing the effects of the present invention, in addition to the above-mentioned medicinal agents. The components used can be blended, for example, water, fats and oils, waxes, hydrocarbons, fatty acids, higher alcohols, esters, plant extracts, water-soluble polymers, surfactants, metal soaps Ingredients such as alcohol, polyhydric alcohol, pH adjuster, preservative, fragrance, powder, thickener, dye, chelating agent can be appropriately blended.

  Examples of the dosage form of the external preparation for skin growth and cosmetics for promoting fibroblast proliferation of the present invention include liquids, ointments, packs, cleaning agents, bathing agents, aerosols, patches, etc. Examples include, but are not limited to, lotions, creams, milky lotions, serums, and makeup cosmetics. Moreover, it can be used for any of cosmetics, quasi drugs, and pharmaceuticals.

If the applied amount of the external preparation for skin growth promotion and cosmetics of the present invention to the epidermis is too small, the effect of the invention cannot be obtained, and if it is too large, the effect commensurate with the added amount cannot be obtained. Therefore, it is preferably 0.0002 to 1340 μg / cm ² · day, more preferably 0.0002 to 270 μg / cm ² · day per unit skin area (1 cm ² ) in terms of specific plant extract (dry solid content). To do.

The cosmetic method of the present invention is characterized by applying the above-mentioned cosmetic for promoting proliferation of fibroblasts to the skin. As a technique for applying the cosmetic for promoting proliferation of fibroblasts to the skin, Depending on the dosage form of the material, an application method, a spray method or the like can be employed. Preferred periods of application include after bathing and before going to bed.

  Next, the present invention will be described more specifically with reference to production examples, test examples, and examples, but the present invention is not limited thereto.

(Production Example) Production of each extract of lentil extract, citrus extract, camellia extract, holy basil:
100 g of each of the lentil seed part, cypress fruit part, cypress whole plant, and holy basil leaf part was pulverized, and 1000 mL of 50 vol% aqueous ethanol solution was added and mixed. It left still at room temperature for 2 days, filtered, the extract was obtained, and this extract was used as a plant extract in the following test examples and Examples.

(Test example) Fibroblast proliferation promoting action A human face skin-derived fibroblast (HDF) was used as a medium, and ES medium (Nissui Pharmaceutical) supplemented with 5% fetal calf serum (FCS) was used. ⁴ × 1 × 10 ^{4 in} a plastic dish and cultured at 37 ° C. for 2 days in a 5 vol% CO ₂ environment. After culturing, the plant extract obtained in Production Example was added to a final concentration of 0.1 μg per mL of medium. The medium and plant extract were changed every three days and cultured for 15 days. After culturing, the cells were detached with trypsin to obtain a cell suspension, and the cell proliferation action was determined by counting the number of living cells using a hemocytometer. The number of cells when no plant extract as a control was added was defined as 100, and the cell growth rate when each plant extract was added was determined. The results of cell proliferation rate are shown in Table 1.

  As is apparent from Table 1, the plant extract characterizing the present invention was found to have a fibroblast growth promoting effect.

Example 1 (Preparation of cream for external use)
Among the blended components in Table 2, the components (1) to (6) are heated and mixed to prepare a mixture 1A at 75 ° C. Separately, the components (7) to (10) are added to the component (12). A mixture 1B at 75 ° C. was prepared by heating and mixing. The resulting mixture 1A and mixture 1B were mixed and emulsified using a homomixer and allowed to cool to room temperature to prepare mixture 1C. Component (11) was uniformly mixed with the obtained mixture 1C to obtain an external cream.

  As component (11), instead of Japanese lentil extract, Japanese extract (Example 1-2), Japanese camellia extract (Example 1-3), and holy basil extract (Example 1-4) were used. A cream for external use was produced in the same manner.

Example 2 (Preparation of lotion)
Among the blending components in Table 3, components (1) to (6) are mixed and dissolved at room temperature to prepare mixture 2A. Separately, components (7) to (13) are mixed and dissolved at room temperature. Mixture 2B was prepared. The obtained mixture 2A and mixture 2B were uniformly mixed using a stirrer to obtain a skin lotion.

Example 3 (Preparation of emulsion)
Among the blending components in Table 4, the components (1) to (8) are heated and mixed to prepare a mixture 3A at 75 ° C. Separately, the components (9) to (13) are added to the component (15). A mixture 3B at 75 ° C. was prepared by heating and mixing. The resulting mixture 3A and mixture 3B were mixed and emulsified using a homomixer and allowed to cool to room temperature to prepare mixture 3C. Component (14) was uniformly mixed with the obtained mixture 3C to obtain an emulsion.

Example 4 (Preparation of pack agent)
Among the blending components in Table 5, the mixture (1) to (6) is heated and dissolved at 70 ° C. while mixing to prepare a mixture 4A. Separately, the components (7) to (10) are mixed at room temperature. The mixture 4B was prepared by mixing and dissolving. The obtained mixture 4A and mixture 4B were mixed using a stirrer and allowed to cool to room temperature to obtain a pack agent.

Example 5 (Preparation of emulsion foundation)
Among the blending components in Table 6, components (1) to (6) were mixed and dissolved at room temperature, and components (12) to (16) were added and mixed by heating to prepare a mixture 5A at 70 ° C. Separately, components (7) to (11) and (18) were heated and mixed to prepare a mixture 5B at 70 ° C. The mixture 5A was added to the resulting mixture 5B, emulsified using a homomixer, and allowed to cool to room temperature to prepare a mixture 5C. Component (17) was uniformly mixed with the obtained mixture 5C to obtain an emulsion foundation.

(Evaluation test)
For the cream shown in Example 1 or Comparative Example 1 (prepared by substituting the plant extract of the present invention in the formulation of Example 1 with purified water), the anti-aging effect was tested and evaluated as described below.

(Evaluation methods)
A group of 20 female subjects (28 to 60 years old) with significant aging symptoms (increased wrinkles, increased sagging, decreased skin elasticity), and each of the creams of Example 1 and Comparative Example 1 was included in each group. 2 times daily (when washing in the morning and after taking a bath in the evening) for 2 months, the beauty specialists observe the degree of improvement of the skin condition before and after use and evaluate the following: Evaluation was made according to criteria. The results obtained are shown in Table 7. In addition, the number in Table 7 is the number of subjects corresponding to the item.

(Evaluation criteria)
Evaluation rank <Contents>
Effective Symptoms improved.
Slightly effective Symptoms improved slightly.
Invalid No improvement is seen.

  As is clear from Table 7, when the cream of Example 1 containing the plant extract characterizing the present invention was used in the wrinkle, sagging, and skin elasticity, the cream of Comparative Example 1 was used. Also, a more remarkable improvement effect was recognized.

  Further, the skin lotions, emulsions, packs, and emulsion foundations of Examples 2 to 5 were also evaluated in the same manner as in Example 1. In each Example, the corresponding agents not containing the plant extract characterizing the present invention were used. A more remarkable improvement effect was observed.

  From the above, it has been clarified that the cosmetic of the present invention has an excellent anti-aging effect on skin aging symptoms such as wrinkles, sagging, and skin elasticity reduction.

  The skin external preparation for promoting fibroblast proliferation and the cosmetic for promoting fibroblast proliferation of the present invention, and the cosmetic method of the present invention using this cosmetic include the metabolism of the dermis by promoting the proliferation action of fibroblasts. Promotes the effects of preventing and improving aging symptoms such as wrinkles, sagging and reduced skin elasticity. Therefore, these agents or cosmetic methods are useful in the medical and cosmetic fields.

Claims (3)

  It contains one or more plant extracts selected from lentil (Lens esculenta), cypress (Piper longum), camellia (Centella asiatica) and holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album) as an active ingredient. A skin external preparation for promoting fibroblast proliferation.   It contains one or more plant extracts selected from lentil (Lens esculenta), cypress (Piper longum), camellia (Centella asiatica) and holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album) as an active ingredient. A cosmetic material for promoting fibroblast proliferation.   A cosmetic method comprising applying the cosmetic according to claim 2 to the skin in order to promote fibroblast proliferation for cosmetic purposes.