JP2008184440A - Skin external preparation for improving UV cell damage - Google Patents
Skin external preparation for improving UV cell damage Download PDFInfo
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Abstract
【課題】日常の紫外線により受けた皮膚細胞の障害を回復させ、若々しい肌の状態の維持を可能とする紫外線細胞障害改善用皮膚外用剤を提供する。
【解決手段】紫外線細胞障害改善用皮膚外用剤が、ヒラマメ、ベンガルボダイジュ、サルサパリラ、ホーリーバジルまたはミムソプス・エレンギから選ばれる一種または二種以上の植物抽出物を有効成分として含有する。
【選択図】なしThe present invention provides an external preparation for ameliorating ultraviolet cell damage that can recover the damage of skin cells caused by daily ultraviolet rays and maintain a youthful skin condition.
A topical skin preparation for improving ultraviolet cell damage contains one or more plant extracts selected from lentil, bengal body, sarsaparilla, holy basil or mimsopus erengi as an active ingredient.
[Selection figure] None
Description
本発明は、ヒラマメ（Lens esculenta）、ベンガルボダイジュ（Ficus benghalensis）、サルサパリラ（Hemidesmus indicus）、ホーリーバジル（Ocimum sanctum, Ocimum tenuiflorum, Ocimum album）、ミムソプス・エレンギ（Mimusops elengi）から選ばれる一種または二種以上の植物抽出物を含有する紫外線細胞障害改善用皮膚外用剤及び化粧料、並びに該化粧料を使用する美容方法に関する。 The present invention is selected from lentil (Lens esculenta), bengal bodaige (Ficus benghalensis), salsaparilla (Hemidesmus indicus), holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album), Mimusops elengi (Mimusops elengi) The present invention relates to a skin external preparation and cosmetic for ultraviolet cell damage improvement containing the above plant extract, and a cosmetic method using the cosmetic.
皮膚の老化には、非露出部にみられる自然老化と、太陽光線露光部にみられる光老化という特徴的な２種が存在する。自然老化は外観的変化が乏しく、機能的な衰退を特徴とする。一方、光老化は微細なしわあるいは深いしわ、色素異常症、黄色調の変化、乾燥、粗ぞうなどの臨床像を特徴とする。紫外線照射が真皮の細胞外基質の崩壊をもたらす（非特許文献１）ように、光老化の主な元凶は紫外線であり、顔面のしわは光老化の代表的特徴である。 There are two characteristic types of skin aging: natural aging seen in unexposed areas and photoaging seen in sun light exposed areas. Natural aging is characterized by poor functional changes and poor functional appearance. On the other hand, photoaging is characterized by clinical features such as fine wrinkles or deep wrinkles, dyschromia, yellowish change, dryness and rough creases. As ultraviolet irradiation causes the extracellular matrix of the dermis to collapse (Non-patent Document 1), the main cause of photoaging is ultraviolet rays, and facial wrinkles are a typical feature of photoaging.
太陽紫外線は、皮膚で吸収された後、脂質の過酸化、転写因子の活性、ＤＮＡの傷害などの細胞障害を引き起こす。これらの細胞障害は基質分解酵素（ＭＭＰｓ）の発現を高め、それによりコラーゲンなどの基質が分解され、減少する。このため、真皮は脆弱になり、しわが形成される。また、紫外線照射によりＤＮＡに障害が起こった細胞では、障害の程度に応じて細胞死（アポトーシス）が誘導され、この細胞死は光老化における重要な役割を果たすと考えられている。したがって、紫外線による細胞障害の防御、改善は、皮膚の光老化の予防、改善につながると考えられる。 Solar ultraviolet rays, after being absorbed by the skin, cause cell damage such as lipid peroxidation, transcription factor activity, and DNA damage. These cellular disorders increase the expression of substrate degrading enzymes (MMPs), thereby degrading and reducing substrates such as collagen. For this reason, the dermis becomes brittle and wrinkles are formed. Further, cell death (apoptosis) is induced in cells in which DNA is damaged by ultraviolet irradiation, depending on the degree of damage, and this cell death is considered to play an important role in photoaging. Therefore, protection and improvement of cell damage by ultraviolet rays is considered to lead to prevention and improvement of photoaging of the skin.
従来、紫外線による細胞障害を予防する方法として、衣類や帽子あるいは日傘などを用いる物理的な遮蔽に加え、サンスクリーン剤の外用が行われている（特許文献１）。 Conventionally, as a method for preventing cell damage due to ultraviolet rays, in addition to physical shielding using clothes, hats, parasols, and the like, sunscreen agents have been applied externally (Patent Document 1).
しかしながら、サンスクリーン剤は、紫外線による細胞障害の予防には有効であっても、使用感の問題や、耐水性の限界から継続的な使用ができず、光接触皮膚炎のような副作用を惹起しやすいという安全性上の問題も有している。また、多くの場合、その効果も万全なものではない。そのため、紫外線による細胞障害を効果的に回復させる紫外線細胞障害改善剤が求められていた。 However, even though sunscreen agents are effective in preventing cell damage caused by ultraviolet rays, they cannot be used continuously due to problems with the feeling of use and water resistance, causing side effects such as photocontact dermatitis. It also has a safety problem that it is easy to do. In many cases, the effect is not perfect. Therefore, there has been a demand for an agent for improving ultraviolet cell damage that effectively recovers cell damage caused by ultraviolet light.
本発明は、前記従来技術に鑑みてなされたものであり、皮膚内の細胞に働きかけることにより、真皮の重要な成分の一つであるコラーゲンの生合成を助け、コラーゲンなどの基質の分解を抑制し、さらに過酸化脂質の生成を抑制することで肌のトラブルを予防するとともに、日常の紫外線により受けた皮膚細胞の障害を回復させ、若々しい肌を維持できるようにする紫外線細胞障害改善用皮膚外用剤及び化粧料を提供し、さらにこの化粧料を用いた美容方法を提供することを目的とする。 The present invention has been made in view of the above prior art, and by acting on the cells in the skin, it helps the biosynthesis of collagen, which is one of the important components of the dermis, and suppresses the degradation of substrates such as collagen. In addition, it prevents skin troubles by suppressing the formation of lipid peroxide, and also helps to repair skin cell damage caused by daily UV rays and maintain youthful skin damage. An object is to provide an external preparation for skin and a cosmetic, and to provide a cosmetic method using the cosmetic.
本発明者らは、上記現況に鑑み、広く植物由来成分の探索を行った結果、ヒラマメ（Lens esculenta）、ベンガルボダイジュ（Ficus benghalensis）、サルサパリラ（Hemidesmus indicus）、ホーリーバジル（Ocimum sanctum, Ocimum tenuiflorum, Ocimum album）、ミムソプス・エレンギ（Mimusops elengi）の各々の抽出物が、予想外にも紫外線細胞障害改善効果を有することを見出し、この知見に基づいて本発明を完成するに至った。 In light of the above-mentioned present situation, the present inventors have extensively searched for plant-derived components. Ocimum album) and Mimusops elengi extract were unexpectedly found to have an effect of improving ultraviolet cell damage, and the present invention was completed based on this finding.
即ち、本発明は、ヒラマメ（Lens esculenta）、ベンガルボダイジュ（Ficus benghalensis）、サルサパリラ（Hemidesmus indicus）、ホーリーバジル（Ocimum sanctum, Ocimum tenuiflorum, Ocimum album）、ミムソプス・エレンギ（Mimusops elengi）から選ばれる一種または二種以上の抽出物を有効成分として含有することを特徴とする紫外線細胞障害改善用皮膚外用剤及び紫外線細胞障害改善用化粧料並びにこの化粧料を皮膚に適用する美容方法を提供する。 That is, the present invention is selected from lentil (Lens esculenta), bengal body (Ficus benghalensis), salsaparilla (Hemidesmus indicus), holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album), Mimusops elengi (Mimusops elengi) Provided are a skin external preparation for improving ultraviolet cell damage, a cosmetic for improving UV cell damage, and a cosmetic method for applying the cosmetic to the skin, which comprises two or more extracts as active ingredients.
なお、本発明において「紫外線細胞障害」とは、紫外線照射が原因となって生じる細胞障害 、特に炎症、過酸化脂質の生成、種々の酵素の失活、ＤＮＡ損傷などの障害を意味し、皮膚組織障害などの組織障害も含む。ここで、細胞は、紫外線照射によって障害が生じる生体細胞である限り特に限定されることはない。 In the present invention, “ultraviolet cell damage” means cell damage caused by ultraviolet irradiation, particularly damage such as inflammation, production of lipid peroxide, inactivation of various enzymes, DNA damage, and the like. Also includes organizational disorders such as organizational disorders. Here, the cells are not particularly limited as long as they are living cells that are damaged by ultraviolet irradiation.
本発明の紫外線細胞障害改善用皮膚外用剤及び紫外線細胞障害改善用化粧料は、それぞれ皮膚に対する安全性の高い植物由来成分であるヒラマメ（Lens esculenta）、ベンガルボダイジュ（Ficus benghalensis）、サルサパリラ（Hemidesmus indicus）、ホーリーバジル（Ocimum sanctum, Ocimum tenuiflorum, Ocimum album）、ミムソプス・エレンギ（Mimusops elengi）から選ばれる一種または二種以上の植物抽出物を、紫外線細胞障害を改善する有効成分として含有するので、皮膚に適用することにより優れた紫外線細胞障害改善効果を発揮する。また、本発明の美容方法は、本発明の紫外線細胞障害改善用化粧料を使用するので、真皮の重要な成分の一つであるコラーゲンの生合成を助け、コラーゲンなどの基質の分解を抑制し、さらに過酸化脂質の生成を抑制することで肌のトラブルを予防することが期待できる。また、日常の紫外線により受けた皮膚細胞の障害を改善し、若々しい肌の状態を維持するという皮膚の光老化の予防、改善効果が期待できる。 The skin external preparation for improving ultraviolet cell damage and the cosmetic for improving UV cell damage of the present invention are plant-derived components with high safety for skin, such as lentil (Lens esculenta), bengal bodaige (Ficus benghalensis), and salsaparilla (Hemidesmus indicus). ), Holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album), Mimusops elengi (Mimusops elengi) one or more kinds of plant extracts are contained as an active ingredient to improve UV cell damage, When applied to, it exhibits an excellent effect of improving ultraviolet cell damage. In addition, since the cosmetic method of the present invention uses the cosmetic material for improving UV cell damage of the present invention, it assists in biosynthesis of collagen, which is one of the important components of the dermis, and suppresses degradation of a substrate such as collagen. Furthermore, it can be expected to prevent skin troubles by suppressing the production of lipid peroxide. In addition, it can be expected to have an effect of preventing and improving the photoaging of the skin by improving skin cell damage caused by daily ultraviolet rays and maintaining a youthful skin condition.
本発明は、ヒラマメ（Lens esculenta）、ベンガルボダイジュ（Ficus benghalensis）、サルサパリラ（Hemidesmus indicus）、ホーリーバジル（Ocimum sanctum, Ocimum tenuiflorum, Ocimum album）、ミムソプス・エレンギ（Mimusops elengi）から選ばれる一種または二種以上の植物抽出物を有効成分として含有する紫外線細胞障害改善用皮膚外用剤、紫外線細胞障害改善用化粧料及びこの化粧料を皮膚に適用する美容方法である。 The present invention is selected from lentil (Lens esculenta), bengal bodaige (Ficus benghalensis), salsaparilla (Hemidesmus indicus), holy basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album), Mimusops elengi (Mimusops elengi) A skin external preparation for improving ultraviolet cell damage containing the above plant extract as an active ingredient, a cosmetic for improving ultraviolet cell damage, and a cosmetic method for applying this cosmetic to the skin.
本発明に用いるヒラマメ（Lens esculenta）はマメ科Lens（レンス）属の植物であり、インド、パキスタンで主に生産され、高栄養食品として知られている。ベンガルボダイジュ（Ficus benghalensis）はクワ科イチジク属の植物であり、熱帯アジアに広く分布する常緑高木として知られている。サルサパリラ（Hemidesmus indicus）はガガイモ科Hemidesmus属の植物であり、約350種が全世界に分布している。ホーリーバジル（Ocimum sanctum, Ocimum tenuiflorum, Ocimum album）は、シソ科メボウキ（オキムム）属の植物であり、英名でHoly Basil、Sacred Basilなどとも呼ばれ、インド、ヨーロッパが原産の一年草である。ミムソプス・エレンギ（Mimusops elengi）はアカテツ科Mimusops属の植物であり、インドからネパールに分布する常緑高木である。これらの植物は、単独で、又は複数種を組み合わせて用いられる。なお、本発明で使用するこれらの植物の産地は特に限定されない。 The lentil (Lens esculenta) used in the present invention is a plant belonging to the genus Lens (Lens), is mainly produced in India and Pakistan, and is known as a highly nutritious food. Bengalbodaiju (Ficus benghalensis) is a plant belonging to the genus Asteraceae and is known as an evergreen tree widely distributed in tropical Asia. Sarsaparilla (Hemidesmus indicus) is a plant belonging to the genus Hemidesmus, about 350 species distributed throughout the world. Holy Basil (Ocimum sanctum, Ocimum tenuiflorum, Ocimum album) is a plant belonging to the genus Lamiaceae (Okimum), also known as Holy Basil and Sacred Basil in English, and is an annual plant native to India and Europe. Mimusops elengi is a plant belonging to the genus Mimusops, an evergreen tree distributed from India to Nepal. These plants are used alone or in combination of a plurality of species. In addition, the production center of these plants used by this invention is not specifically limited.
本発明で利用する各植物（ヒラマメ、ベンガルボダイジュ、サルサパリラ、ホーリーバジル、ミムソプス・エレンギ）の抽出物としては、それぞれの植物体の葉、茎、幹、樹皮、幼芽、花、果実、種子、根等の植物体の一部位又は複数部位の混合あるいは全草から抽出したものを使用できるが、好ましくは、ヒラマメの場合には種子が用いられ、ベンガルボダイジュの場合には新芽、若葉が用いられ、サルサパリラの場合には全草が用いられ、ホーリーバジルの場合には葉が用いられ、ミムソプス・エレンギの場合には果実が用いられる。 As an extract of each plant used in the present invention (sole bean, bengal body, salsaparilla, holy basil, mimsopus erengi), leaves, stems, stems, bark, buds, flowers, fruits, seeds, A mixture of one part or a plurality of parts of a plant body such as a root or a plant extracted from whole grass can be used. Preferably, seeds are used in the case of Japanese lentils, and new shoots and young leaves are used in the case of Bengalbodaiju. In the case of Sarsaparilla, the whole plant is used, in the case of Holy Basil, the leaves are used, and in the case of Mimsopus erengi, the fruits are used.
抽出は、前記植物体の各部位を生のまま用いてもよいが、乾燥、細切、粉砕、圧搾または発酵等の前処理を適宜に施した後、適宜低温ないし加温した溶媒を用いて抽出することが好ましい。その抽出方法は特に限定されないが、例えば、上記植物体の一部位、または二種以上の部位を、低温乃至加温下の溶媒中に浸漬する方法があげられる。抽出時間は抽出溶媒の種類や抽出温度に応じて設定されるが、１時間から２週間程度が好ましい。 For extraction, each part of the plant body may be used as it is, but after appropriately performing pretreatment such as drying, chopping, crushing, pressing or fermentation, using a solvent that is appropriately cold or warm. It is preferable to extract. The extraction method is not particularly limited, and examples thereof include a method of immersing one part or two or more parts of the plant in a solvent at a low temperature to a warm temperature. The extraction time is set according to the type of extraction solvent and the extraction temperature, but is preferably about 1 to 2 weeks.
抽出溶媒としては、例えば水、低級１価アルコール（メチルアルコール、エチルアルコール、１−プロパノール、２−プロパノール、１−ブタノール、２−ブタノール等）、液状多価アルコール（グリセリン、プロピレングリコール、ジプロピレングリコール、１，３−ブチレングリコール等）、低級エステル（酢酸エチル、酢酸ブチル等）、炭化水素（ベンゼン、ヘキサン、ペンタン等）、ケトン類（アセトン、メチルエチルケトン等）、エーテル類（ジエチルエーテル、テトラヒドロフラン、ジプロピルエーテル等）、アセトニトリル等が挙げられ、それらの一種又は二種以上を用いることができる。また、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水等を用いてもよい。中でも、低級アルコールを使用することが好ましい。
また、各植物の有効成分の抽出液には、必要に応じて、本発明の効果に影響のない範囲で更に、脱臭、脱色等の精製処理を行ってもよい。更に、必要により防腐防黴剤（フェノキシエタノール、パラオキシ安息香酸エステル類等）を添加してもよい。
Examples of the extraction solvent include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, dipropylene glycol). , 1,3-butylene glycol etc.), lower esters (ethyl acetate, butyl acetate etc.), hydrocarbons (benzene, hexane, pentane etc.), ketones (acetone, methyl ethyl ketone etc.), ethers (diethyl ether, tetrahydrofuran, di Propyl ether, etc.), acetonitrile and the like, and one or more of them can be used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Among these, it is preferable to use a lower alcohol.
In addition, the extract of the active ingredient of each plant may be further subjected to purification treatment such as deodorization and decolorization as long as it does not affect the effects of the present invention. Furthermore, if necessary, antiseptic / antifungal agents (phenoxyethanol, paraoxybenzoates, etc.) may be added.
各植物の好ましい抽出方法の例としては、濃度０〜１００ｖｏｌ％の含水エチルアルコール又は１，３−ブチレングリコールを用い、室温、又は３０〜４５℃に加温して１〜１０日間放置することにより抽出後ろ過し、得られたろ液を更に１週間程放置して熟成させ、再びろ過を行う方法が挙げられる。 As an example of a preferable extraction method for each plant, water-containing ethyl alcohol or 1,3-butylene glycol having a concentration of 0 to 100 vol% is used, and the mixture is allowed to stand at room temperature or 30 to 45 ° C. and left for 1 to 10 days. There is a method of filtering after extraction and allowing the filtrate obtained to stand for another week for aging, followed by filtration again.
紫外線細胞障害改善用皮膚外用剤または紫外線細胞障害改善用化粧料におけるヒラマメ抽出物、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物の合計の含有量は、乾燥固形分として好ましくは０．００００１〜１０質量％であり、より好ましくは０．００００１〜２質量％である。ヒラマメ抽出物、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物から選ばれる一種または二種以上の合計の含有量がこの範囲内であれば、植物抽出物を安定に配合することができ、皮膚への安全性も高く、かつ高い薬効効果乃至美容効果を発揮することができる。 The total content of Japanese lentil extract, Bengal bouillade extract, Sarsaparilla extract, Holy basil extract and Mimsopus erengi extract in the skin external preparation for improving UV cell damage or cosmetics for improving UV cell damage is the dry solid content. Preferably, it is 0.00001-10 mass%, More preferably, it is 0.00001-2 mass%. If the total content of one kind or two or more kinds selected from lentil extract, bengal body sage extract, salsaparilla extract, holy basil extract and mimsopus erengi extract is within this range, the plant extract can be stabilized. It can be blended, has high safety to the skin, and can exhibit high medicinal and cosmetic effects.
また、本発明において、紫外線細胞障害改善用皮膚外用剤又は紫外線細胞障害改善用化粧料は、通常用いられる各種の薬効剤、例えば、美白剤、抗酸化剤、抗炎症剤、細胞賦活剤、紫外線防止剤、保湿剤、血行促進剤等から選ばれる薬効剤の一種又は二種以上と併用することができる。それにより、本発明の効果をより高めることが可能である。 Further, in the present invention, the skin external preparation for improving ultraviolet cell damage or the cosmetic for improving UV cell damage are various commonly used medicinal agents such as whitening agents, antioxidants, anti-inflammatory agents, cell activators, ultraviolet rays. It can be used in combination with one or more medicinal agents selected from inhibitors, humectants, blood circulation promoters, and the like. Thereby, the effect of the present invention can be further enhanced.
本発明の紫外線細胞障害改善用皮膚外用剤、紫外線細胞障害改善用化粧料には、上述の薬効剤以外に、本発明の効果を損なわない範囲内で、外用剤又は化粧料に通常用いられる成分を配合することができ、例えば、水、油脂類、ロウ類、炭化水素類、脂肪酸類、高級アルコール類、エステル類、植物抽出エキス類、水溶性高分子、界面活性剤、金属石鹸、アルコール、多価アルコール、ｐＨ調整剤、防腐剤、香料、粉体、増粘剤、色素、キレート剤等の成分を適宜配合することができる。 In addition to the above-mentioned medicinal agents, the skin external preparation for improving UV cell damage and cosmetics for improving UV cell damage of the present invention are components that are usually used in external preparations or cosmetics, as long as the effects of the present invention are not impaired. For example, water, fats and oils, waxes, hydrocarbons, fatty acids, higher alcohols, esters, plant extracts, water-soluble polymers, surfactants, metal soaps, alcohols, Components such as polyhydric alcohols, pH adjusters, preservatives, fragrances, powders, thickeners, dyes, and chelating agents can be appropriately blended.
本発明の紫外線細胞障害改善用皮膚外用剤及び紫外線細胞障害改善用化粧料の剤形としては、例えば、軟膏、液剤、パック、洗浄料、浴用剤、軟膏、液剤、エアゾール、貼付剤等が挙げられ、特に化粧料としては、化粧水、クリーム、乳液、美容液、メーキャップ化粧料が挙げられるが、これらに限定されるものではない。また、化粧品、医薬部外品、医薬品のいずれにも用いることができる。 Examples of the dosage form of the skin external preparation for improving UV cell damage and the cosmetic for improving UV cell damage of the present invention include ointments, solutions, packs, cleaning agents, bath preparations, ointments, solutions, aerosols, patches and the like. In particular, examples of cosmetics include, but are not limited to, lotions, creams, milky lotions, cosmetics, and makeup cosmetics. Moreover, it can be used for any of cosmetics, quasi drugs, and pharmaceuticals.
本発明の紫外線細胞障害改善用皮膚外用剤及び紫外線細胞障害改善用化粧料の表皮に対する適用量は、少なすぎると発明の効果が得られず、多すぎても添加量に見合った効果が得られない。従って、特定植物抽出物（乾燥固形分）換算で、表皮単位面積（１ｃｍ２）当たり、好ましくは０．０００２〜１３４０μｇ／ｃｍ２・ｄａｙ、より好ましくは０．０００２〜２７０μｇ／ｃｍ２・ｄａｙとする。 The applied amount of the skin external preparation for improving UV cell damage and cosmetics for improving UV cell damage of the present invention to the epidermis is too small to obtain the effects of the invention, and even if it is too much, an effect commensurate with the added amount is obtained. Absent. Therefore, it is preferably 0.0002 to 1340 μg / cm 2 · day, more preferably 0.0002 to 270 μg / cm 2 · day per unit skin area (1 cm 2 ) in terms of specific plant extract (dry solid content). To do.
また、本発明の美容方法は、上述の紫外線細胞障害改善用化粧料を皮膚に適用することを特徴としているが、紫外線細胞障害改善用化粧料を皮膚に適用する手法としては、当該化粧料の剤形に応じて塗布法やスプレー法などを採用することができる。好ましい適用の時期としては、入浴後就寝前等がある。 In addition, the cosmetic method of the present invention is characterized by applying the above-mentioned cosmetic for improving ultraviolet cell damage to the skin. As a method for applying the cosmetic for improving ultraviolet cell damage to the skin, Depending on the dosage form, an application method, a spray method or the like can be employed. Preferred periods of application include after bathing and before going to bed.
次に、製造例、試験例、実施例を挙げて本発明を更に具体的に説明するが、本発明はこれらに何ら制約されるものではない。 Next, the present invention will be described more specifically with reference to production examples, test examples, and examples, but the present invention is not limited thereto.
（製造例）ヒラマメ抽出物、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ミムソプス・エレンギ抽出物、ホーリーバジル抽出物の製造：
ヒラマメの種子部位、ベンガルボダイジュの葉部位、サルサパリラの全草、ミムソプス・エレンギの果実部位、ホーリーバジルの葉部位のそれぞれ１００ｇを粉砕し、５０ｖｏｌ％含水エタノール溶液１０００ｍＬを加え混合した。室温にて２日間静置し、ろ過して抽出液を得、この抽出液を以降の試験例及び実施例の植物抽出物として用いた。
(Production Example) Production of Japanese lentil extract, Bengal body extract, Sarsaparilla extract, Mimpus erengi extract, Holy basil extract:
100 g of each of the lentil seed part, the leaf part of Bengalbodaiju, the whole plant of Salsaparilla, the fruit part of Mimsopus erengi, and the leaf part of holy basil were pulverized, and 1000 mL of 50 vol% aqueous ethanol solution was added and mixed. It left still at room temperature for 2 days, filtered, the extract was obtained, and this extract was used as a plant extract of a subsequent test example and an Example.
（試験例１） 紫外線細胞障害回復作用
胎児肺由来の線維芽細胞（ＴＩＧ−７）を、培地として、ウシ胎仔血清（ＦＣＳ）を５％添加したＥＳ培地（日水製薬）を用いて、直径３５ｍｍのプラスチックディッシュに１×１０４個ずつ播種し、５ｖｏｌ％ＣＯ２環境下、３７℃で２日間培養した。培養後、紫外線（強度７Ｊ／ｍ２）を照射した。紫外線照射後、製造例で得たヒラマメ、ベンガルボダイジュ、ミムソプス・エレンギの各植物抽出物をそれぞれ最終濃度で培地１ｍＬあたり０．１μｇとなるよう添加した。培地と植物抽出物を三日毎に交換し、一週間培養した。培養後、細胞をトリプシンで剥がして細胞浮遊液とし、血球計算盤を用いて生細胞数を計測することにより細胞障害回復作用を判定した。対照である植物抽出物無添加時の細胞数を１００とした時の、各植物抽出物添加時の生細胞数の比を求め、この値を細胞障害回復率とした。細胞障害回復率の結果を表１に示す。
Test Example 1 UV Cell Damage Recovery Action Fetal lung-derived fibroblasts (TIG-7) were used as the medium, and ES medium (Nissui Pharmaceutical) supplemented with 5% fetal calf serum (FCS) was used. Four 1 × 10 4 seeds were seeded on a 35 mm plastic dish and cultured at 37 ° C. for 2 days in a 5 vol% CO 2 environment. After the cultivation, ultraviolet rays (intensity 7 J / m 2 ) were irradiated. After the ultraviolet irradiation, each plant extract of lentil, bengalbodaiju, mimsopus erengi obtained in Production Example was added to a final concentration of 0.1 μg per 1 mL of the medium. The medium and plant extract were changed every three days and cultured for one week. After culturing, the cells were detached with trypsin to obtain a cell suspension, and the cell damage recovery action was determined by measuring the number of living cells using a hemocytometer. The ratio of the number of living cells when each plant extract was added when the number of cells when no plant extract was added as a control was defined as 100, and this value was taken as the cell damage recovery rate. Table 1 shows the results of the cytotoxicity recovery rate.
（試験例２） 紫外線細胞障害回復作用
試験例１において、胎児肺由来の線維芽細胞（ＴＩＧ−７）に代えてヒト顔皮膚由来の線維芽細胞（ＨＤＦ）を使用し、培養後照射する紫外線強度を７Ｊ／ｍ２に代えて強度１０Ｊ／ｍ２とし、植物抽出物としてベンガルボダイジュ、サルサパリラ、ホーリーバジルの各植物抽出物を使用し、試験例１と同様に細胞障害回復率を求めた。結果を表２に示す。
(Test Example 2) UV cell injury recovery action In Test Example 1, human face skin-derived fibroblasts (HDF) were used instead of fetal lung-derived fibroblasts (TIG-7), and UV irradiation was performed after culture. and strength 10J / m 2 in place of the intensity 7J / m 2, using Bengal lime, sarsaparilla, each plant extract of Holy basil as plant extracts, was similarly determined cytotoxic recovery rate as in test example 1. The results are shown in Table 2.
表１及び表２から明らかなように、本発明を特徴づける植物抽出物を添加することにより、紫外線細胞障害に対して回復作用が認められ、各植物抽出物は紫外線細胞障害を改善する有効成分であることが明らかとなった。 As is clear from Tables 1 and 2, by adding the plant extract characterizing the present invention, a recovery action against ultraviolet cell damage is recognized, and each plant extract is an active ingredient that improves UV cell damage. It became clear that.
実施例１（液状外用剤の調整）
表３の配合成分を均一に混合溶解して液状外用剤を調整した。
Example 1 (Preparation of liquid external preparation)
A liquid external preparation was prepared by uniformly mixing and dissolving the blending components shown in Table 3.
成分（５）として、ヒラマメ抽出物に代えて、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物をそれぞれ使用して外用剤を同様に製造した。 External preparations were prepared in the same manner by using, as component (5), bengalbo daiju extract, salsaparilla extract, holy basil extract and mimsop elengi extract instead of lentil extract.
実施例２（外用クリーム剤の調製）
表４の配合成分のうち、成分（１）〜（６）を加熱混合して７５℃の混合物１Ａを調製し、それとは別に、成分（１２）に成分（７）〜（１０）を加えて加熱混合して７５℃の混合物１Ｂを調製した。得られた混合物１Ａと混合物１Ｂとを、ホモミキサーを用いて混合乳化し、室温まで放冷することで混合物１Ｃを調製した。得られた混合物１Ｃに、成分（１１）を均一に混合して外用クリーム剤を得た。
Example 2 (Preparation of cream for external use)
Among the blending components in Table 4, the components (1) to (6) are heated and mixed to prepare a mixture 1A at 75 ° C. Separately, the components (7) to (10) are added to the component (12). A mixture 1B at 75 ° C. was prepared by heating and mixing. The resulting mixture 1A and mixture 1B were mixed and emulsified using a homomixer and allowed to cool to room temperature to prepare mixture 1C. Component (11) was uniformly mixed with the obtained mixture 1C to obtain an external cream.
成分（１１）として、ヒラマメ抽出物に代えて、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物をそれぞれ使用して外用クリーム剤を同様に製造した。 A cream for external use was prepared in the same manner by using, as component (11), a bengal body extract, a salsaparilla extract, a holy basil extract and a mimsop elengi extract instead of the lentil extract.
実施例３（化粧水の調製）
表５の配合成分のうち、成分（１）〜（６）を室温下で混合溶解して混合物２Ａを調製し、それとは別に、成分（７）〜（１３）を室温下で混合溶解して混合物２Ｂを調製した。得られた混合物２Ａと混合物２Ｂとを、撹拌機を用いて均一に混合することで化粧水を得た。
Example 3 (Preparation of lotion)
Among the blended components in Table 5, the components (1) to (6) are mixed and dissolved at room temperature to prepare a mixture 2A. Separately, the components (7) to (13) are mixed and dissolved at room temperature. Mixture 2B was prepared. The obtained mixture 2A and mixture 2B were uniformly mixed using a stirrer to obtain a skin lotion.
成分（１２）として、ヒラマメ抽出物に代えて、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物をそれぞれ使用して化粧水を同様に製造した。 As component (12), a lotion was prepared in the same manner using Bengalvo Daiju extract, Sarsaparilla extract, Holy basil extract, and Mimsopus erengi extract, respectively, instead of lentil extract.
実施例４（乳液の調製）
表６の配合成分のうち、成分（１）〜（８）を加熱混合して７５℃の混合物３Ａを調製し、それとは別に、成分（１５）に成分（９）〜（１３）を加えて加熱混合して７５℃の混合物３Ｂを調製した。得られた混合物３Ａと混合物３Ｂとを、ホモミキサーを用いて混合乳化し、室温まで放冷することで混合物３Ｃを調製した。得られた混合物３Ｃに、成分（１４）を均一に混合して乳液を得た。
Example 4 (Preparation of emulsion)
Among the blending components in Table 6, the components (1) to (8) are heated and mixed to prepare a mixture 3A at 75 ° C. Separately, the components (9) to (13) are added to the component (15). A mixture 3B at 75 ° C. was prepared by heating and mixing. The resulting mixture 3A and mixture 3B were mixed and emulsified using a homomixer and allowed to cool to room temperature to prepare mixture 3C. Component (14) was uniformly mixed with the obtained mixture 3C to obtain an emulsion.
成分（１４）として、ヒラマメ抽出物に代えて、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物をそれぞれ使用して乳液を同様に製造した。 As component (14), instead of lentil extract, Bengal boudiju extract, Sarsaparilla extract, Holy basil extract, and Mimsopus erengi extract were similarly prepared.
実施例５（パック剤の調製）
表７の配合成分のうち、成分（１）〜（６）を混合しながら７０℃に加熱し溶解して混合物４Ａを調製し、それとは別に、成分（７）〜（１０）を室温下で混合溶解して混合物４Ｂを調製した。得られた混合物４Ａと混合物４Ｂとを、撹拌機を用いて混合し、室温まで放冷することでパック剤を得た。
Example 5 (Preparation of pack agent)
Among the blending components in Table 7, the mixture (1) to (6) is heated and dissolved at 70 ° C. while mixing to prepare the mixture 4A. Separately, the components (7) to (10) are mixed at room temperature. The mixture 4B was prepared by mixing and dissolving. The obtained mixture 4A and mixture 4B were mixed using a stirrer and allowed to cool to room temperature to obtain a pack agent.
成分（７）として、ヒラマメ抽出物に代えて、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物をそれぞれ使用してパック剤を同様に製造した。 A pack was prepared in the same manner by using, as component (7), bengalbodaiju extract, salsaparilla extract, holy basil extract, and mimsopus erengi extract instead of lentil extract.
実施例６（乳液状ファンデーションの調製）
表８の配合成分のうち、成分（１）〜（６）を室温下で混合溶解し、更に、成分（１２）〜（１６）を加えて加熱混合して７０℃の混合物５Ａを調製した。それとは別に、成分（７）〜（１１）及び（１８）を加熱混合して７０℃の混合物５Ｂを調製した。得られた混合物５Ｂに混合物５Ａを添加し、ホモミキサーを用いて乳化し、室温まで放冷して混合物５Ｃを調製した。得られた混合物５Ｃに、成分（１７）を均一に混合して乳液状ファンデーションを得た。
Example 6 (Preparation of emulsion foundation)
Among the blending components in Table 8, components (1) to (6) were mixed and dissolved at room temperature, and components (12) to (16) were further added and mixed by heating to prepare a mixture 5A at 70 ° C. Separately, components (7) to (11) and (18) were heated and mixed to prepare a mixture 5B at 70 ° C. The mixture 5A was added to the resulting mixture 5B, emulsified using a homomixer, and allowed to cool to room temperature to prepare a mixture 5C. Component (17) was uniformly mixed with the obtained mixture 5C to obtain an emulsion foundation.
成分（１７）として、ヒラマメ抽出物に代えて、ベンガルボダイジュ抽出物、サルサパリラ抽出物、ホーリーバジル抽出物及びミムソプス・エレンギ抽出物をそれぞれ使用して乳液状ファンデーションを同様に製造した。 An emulsion foundation was prepared in the same manner by using, as component (17), bengalbodaiju extract, salsaparilla extract, holy basil extract and mimsopus erengi extract instead of lentil extract.
上記実施例１〜６の各皮膚外用剤あるいは化粧料は、いずれも紫外線により皮膚細胞が受けた障害を効果的に回復させ、若々しい肌状態の維持に有用であった。 Each of the external preparations for skin or cosmetics of Examples 1 to 6 was useful for maintaining the youthful skin condition by effectively recovering the damage received by the skin cells by ultraviolet rays.
本発明の紫外線細胞障害改善用皮膚外用剤、紫外線細胞障害改善用化粧料及びこの化粧料を皮膚に適用する美容方法は、日常の紫外線により受けた皮膚細胞の障害を改善し、若々しい肌の状態を維持することに優れた効果を発揮する。従って、これらの剤又は美容方法は、医療及び美容分野で有用である。 The skin external preparation for improving ultraviolet cell damage, the cosmetic for improving UV cell damage, and the cosmetic method for applying this cosmetic to the skin of the present invention improve skin cell damage caused by daily ultraviolet rays, and provide youthful skin. Excellent effect in maintaining the state of. Therefore, these agents or cosmetic methods are useful in the medical and cosmetic fields.
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| JP2009184997A (en) * | 2008-02-08 | 2009-08-20 | B & C Laboratories Inc | Collagen production promoter |
| WO2010063977A1 (en) * | 2008-12-05 | 2010-06-10 | Societe Industrielle Limousine D'application Biologique, Dite Silab | Cosmetic use of skin cell autophagy activators |
| US20120237459A1 (en) * | 2011-02-22 | 2012-09-20 | Cheri Lynn Swanson | Methods for Improving the appearance of hyperpigmented skin using a synergistic composition comprising banyan tree, lotus, and clover serum fractions |
| KR20140012091A (en) * | 2011-02-22 | 2014-01-29 | 아크조 노벨 케미칼즈 인터내셔널 비.브이. | Composition comprising banyan tree, lotus, and clover serum fractions (hyperpigmentation) |
| JP2014506595A (en) * | 2011-02-22 | 2014-03-17 | アクゾ ノーベル ケミカルズ インターナショナル ベスローテン フエンノートシャップ | A composition containing a serum fraction of Bengal bodyfish, lotus and clover (aging) |
| WO2017077004A1 (en) | 2015-11-05 | 2017-05-11 | Università degli Studi di Camerino | Lentil extract with cholesterol lowering and prebiotic activity |
| CN107296772A (en) * | 2017-08-01 | 2017-10-27 | 广东润洁日化有限公司 | A kind of skin care product containing holy basil extract and its preparation |
| CN107412056A (en) * | 2017-08-01 | 2017-12-01 | 广东润洁日化有限公司 | A kind of washing product containing holy basil extract and its preparation |
| WO2022030803A1 (en) * | 2020-08-07 | 2022-02-10 | 재단법인 전남바이오산업진흥원 | Cosmetic composition for preventing and ameliorating skin aging caused by ultraviolet light, containing persicaria senticosa(meisn.) h.gross ex nakai extract |
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| JP2009184997A (en) * | 2008-02-08 | 2009-08-20 | B & C Laboratories Inc | Collagen production promoter |
| US9138400B2 (en) | 2008-12-05 | 2015-09-22 | Societe Industrielle Limousine D'application Biologique Dite Silab | Cosmetic use of skin cell autophagy activators |
| US8512764B2 (en) | 2008-12-05 | 2013-08-20 | Societe Industrielle Limousine D'application Biologique Dite Silab | Cosmetic use of skin cell autophagy activators |
| FR2939316A1 (en) * | 2008-12-05 | 2010-06-11 | Limousine D Applic Biolog Dite | COSMETIC USE OF ACTIVATORS OF AUTOPHAGIA OF SKIN CELLS. |
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| US9993508B2 (en) | 2011-02-22 | 2018-06-12 | Isp Investments Llp | Composition comprising banyan tree, lotus, and clover serum fractions (aging) |
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| WO2017077004A1 (en) | 2015-11-05 | 2017-05-11 | Università degli Studi di Camerino | Lentil extract with cholesterol lowering and prebiotic activity |
| CN107296772A (en) * | 2017-08-01 | 2017-10-27 | 广东润洁日化有限公司 | A kind of skin care product containing holy basil extract and its preparation |
| CN107412056A (en) * | 2017-08-01 | 2017-12-01 | 广东润洁日化有限公司 | A kind of washing product containing holy basil extract and its preparation |
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| CN107412056B (en) * | 2017-08-01 | 2020-10-16 | 广东润洁日化有限公司 | A kind of washing article containing holy basil extract and preparation thereof |
| WO2022030803A1 (en) * | 2020-08-07 | 2022-02-10 | 재단법인 전남바이오산업진흥원 | Cosmetic composition for preventing and ameliorating skin aging caused by ultraviolet light, containing persicaria senticosa(meisn.) h.gross ex nakai extract |
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