KR101769422B1 - Multifunctional serum composition and preparing method of the same - Google Patents

Abstract

One embodiment of the present invention relates to a combination composition of a serum-type cosmetic composition comprising adenosine, glutamate extract, peptides, niacinamide, gold nodule extract, chestnut extract, galactomisses fermented filtrate, chaga mushroom extract, Wherein the active ingredient is selected from the group consisting of mulberry and licorice mixed extracts, ferulic acid, bifidobacteria extract, aloe vera leaf extract, panthenol, sodium hypalulonate and polyquaternium-51 as active ingredients, Enhancing, wrinkle-improving, skin-lifting, whitening, moisturizing, moisturizing, moisturizing, moisturizing, moisturizing, and so on, including purified water for 2 to 10 wt%, 5 to 15 wt.%, 2 to 10 wt.% Of solubilizing agent, 2 to 15 wt. Functional cosmetic composition, and a method for producing the same, which are capable of complex improvement of various skin problem factors such as pore size reduction, antioxidative effect and the like.

Description

TECHNICAL FIELD The present invention relates to a multifunctional serum composition and a preparation method thereof,

The present invention relates to a mixed composition of multi-functional serum-type cosmetics and a method for producing the same. More specifically, the present invention relates to a method for producing a multifunctional serum-type cosmetic composition comprising adenosine, Multifunctional Serum-type cosmetics with active ingredient, Golden Rhododendron, Huang Baek, Mung bean, Licorice mixed extract, Ferulic acid, Bifidobacteria extract, Aloe vera leaf extract, Panthenol, Sodium hyaluronate, Polyquaternium-51 And a process for producing the same.

In recent years, interest in skin care and skin care has been rising due to the improvement of living standards. In order to meet various consumers' preferences, skin lotion, essence, moisture cream, oil cream, eye cream, neck cream, Cosmetics manufactured in various formulations such as pessary night are commercially available.

In addition, consumers do not require only one of the effects of skin improvement of various cosmetics such as whitening, delayed aging, suppression of wrinkles, improvement of elasticity, reduction of pores, improvement of skin texture, and there is an increasing need to solve complex skin troubles Trend.

On the other hand, cosmetics manufacturers and distributors are currently manufacturing and supplying cosmetics with simple functions for each type of formulation. Accordingly, users of cosmetics have time and economic troubles and troubles due to the application of cosmetics having different functions to the skin in various stages in order to satisfy all desired skin improvement functions, and the cosmetics having different formulations and ingredients Which is caused by the application to the skin of the skin.

Therefore, there is a demand for research and development of a cosmetic product which is stable so that a single product can improve complex skin troubles while causing no skin problems.

Korean Patent Laid-Open Publication No. 2005-0099332 (the name of the invention: multifunctional cosmetic composition, hereinafter referred to as prior art 1) relates to a cosmetic composition comprising 1 (one) selected from the group consisting of charcoal powder, loess powder, tourmaline powder, potassium feldspar powder and mud powder 10 to 40 parts by weight of a functional filler having a particle size of 300 to 400 mesh as a kind or more, and 5 to 10 parts by weight of a complex plant extract in which various kinds of plant extracts are mixed is disclosed have.

Korean Patent Laid-Open No. 10-1223683 (title of the invention: a whitening cosmetic composition containing an East Asian Extract, a method for producing the same, and a cosmetic using the composition, hereinafter referred to as Prior Art 2) The present invention relates to a method for producing a cosmetic composition for use in cosmetics, and more particularly, to a cosmetic composition for cosmetics using Dong-A and Dong-A seeds which can help skin whitening, The present invention relates to a cosmetic composition for whitening comprising an East Asian extract which can be further improved in skin improving effect by carefully selecting a natural material as a material and fermenting it for a certain period of time by putting it in the inside of the East Asian body There is one.

KR 2005-0099332 KR 10-1223683

The prior art 1 discloses a technique relating to a multifunctional cosmetic composition comprising a functional filler and a complex plant extract and has a skin improving effect such as far-infrared and anion release, activation of skin physiological function, moisturizing, antibacterial, deodorization, , And a combination of plant extracts and has an aromatherapy effect. However, the complex plant extract alone may be insufficient for securing a complex skin improving effect. Prior art 1 is a clinical test of a multi-functional cosmetic composition, and only the aromatherapy effect is confirmed. Has not been specifically identified.

The prior art 2 discloses a technique relating to a cosmetic composition containing an East Asian extract, but it is disadvantageous in that it is not suitable for consumers' desire to improve various skin problems with a cosmetic composition having skin whitening and antibacterial functions.

Therefore, the technical problem to be solved by the present invention is to extract a substance applicable to cosmetics from a herb that is not used in character and apply it as an effective substance of cosmetics, and to produce a desired formulation, To prepare a cosmetic composition.

The present invention also relates to a method for the production of a fermented beverage comprising the steps of: adenosine, glutinous extract, peptides, niacinamide, ginseng nest extract, chrysanthemum extract, galactomyces fermented filtrate, chaga mushroom extract, The present invention relates to a mixed composition of a serum-type cosmetic composition capable of simultaneously improving various skin problems using an active ingredient of bifidobacteriaceae, aloe vera leaf extract, panthenol, sodium hyaluronate and polyquaternium-51 as active ingredients and a process for producing the same Another aim is to provide technology.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

In order to accomplish the above object, an embodiment of the present invention is to provide a mixed composition of a serum-type cosmetic composition, which comprises adenosine, glutamate extract, peptides, niacinamide, gold nodule extract, chestnut extract, galactomisses fermented filtrate, The present invention relates to a pharmaceutical composition comprising, as an active ingredient, an active ingredient selected from the group consisting of mushroom extract, golden thyme, yellowtail, mung bean and licorice mixed extract, ferulic acid, bifidobacteriaceae, aloe vera leaf extract, panthenol, sodium hypalulonate and polyquaternium- Characterized in that it comprises purified water so that the total amount of the composition is 14.5 to 48 wt%, 2 to 10 wt% of the skin softening agent, 5 to 15 wt% of the increasing agent, 2 to 10 wt% of the solubilizing agent, 2 to 15 wt% Functional cosmetic composition.

Also, in the mixed composition of the serum type cosmetic according to the embodiment of the present invention, the peptides are selected from the group consisting of caprate peptide-1, aldec-oligopeptide-1 and acetylhexapeptide-8, and S-human oligopeptide-1 And at least one kind selected from the group consisting of

In addition, in the mixed composition of the serum-type cosmetic composition according to an embodiment of the present invention, the skin-softening agent may include at least one selected from heptylundecylenate and caprylic / capric triglyceride have.

Further, in the mixed composition of the serum type cosmetic composition according to an embodiment of the present invention, the thickening agent may include at least one selected from an acrylate / C10-30 alkyl acrylate crosspolymer, xanthan gum, and carbomer .

In addition, in the mixed composition of the serum type cosmetic composition according to an embodiment of the present invention, the solubilizing agent may include at least one selected from polysorbate 80 and olive oil phage-7 ester.

In addition, in the mixed composition of the serum-type cosmetic composition according to an embodiment of the present invention, the additive may include at least one selected from a preservative, a sequestering agent, a pH adjusting agent, and a flavoring agent.

Also, in the method for preparing a multi-functional serum-type cosmetic composition according to an embodiment of the present invention, the raw materials of the multi-functional serum-type cosmetic composition may be selected from the group consisting of golden, yellow, Amide, acrylate / C10-30 alkyl acrylate crosspolymer, xanthan gum, aloe vera leaf extract, carbomer, polyquaternium-51, gold nest extract, disodium edithia, ferulic acid, chaga extract, Gal A first step of preparing a water-based mixture by mixing lactomes fermented filtrate, Bifidobacterium flavum, and a sequestering agent; a step of extracting liposomes, polysorbate 80, olive Oil phase-7 ester, chestnut oil extract, caprylic / capric triglyceride, and heptyl undecylenate to prepare an oil-in-water mixture A third step of adding an oil-in-water mixture from step 2 and a second step to the water-based mixture of the first step and mixing with stirring at 70 to 90 DEG C to prepare an oil-water mixture; lowering the temperature of the oil- And a fourth step of mixing and mixing panthenol, sodium hyaluronate, peptides, adenosine, and an additive, followed by stirring and mixing.

The functional composition of the multifunctional serum type cosmetic composition according to the embodiment of the present invention can be applied to a variety of functional products having different efficacies such as moisturizing, elasticity enhancement, wrinkle improvement, skin lifting, whitening, And anti-inflammatory effect, and it is advantageous in that it does not cause skin irritation because it is not cytotoxic.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

Hereinafter, the present invention will be described with reference to specific examples and experimental examples. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The multifunctional serum-type cosmetic composition according to the present invention includes functional materials having different functions, and thus has moisturizing, wrinkling, elasticity, skin lifting, whitening, pore reduction, antioxidation, anti-inflammation and antibacterial function simultaneously . Specifically, one embodiment of the serum type cosmetic composition according to the present invention is a cosmetic composition comprising at least one of adenosine, glutamate extract, peptides, niacinamide, ginseng nest extract, chestnut extract, galactomisses fermented filtrate, chaga mushroom extract, Wherein the effective ingredient is selected from the group consisting of an effective ingredient of 14.5 to 48 wt%, a skin-softening agent 2, an antioxidant, To 10 wt%, 5 wt% to 5 wt%, 2 wt% to 10 wt% of the solubilizing agent, 2 wt% to 15 wt% of the additive, and 100 wt% of the total mixed composition.

Hereinafter, the multifunctional serum-type cosmetic composition according to the present invention will be described in detail for each component in the above-described manner.

In the present invention, adenosine binds adenine and ribose components, and is present in a small amount in all organisms such as plants, plants and microorganisms. It is a wrinkle-improving ingredient certified by a pharmacist and promotes the synthesis of proteins in the dermal layer of skin, Thereby improving wrinkles, improving elasticity, and lifting the skin. In order to perform the above function, adenosine should be contained at 0.04 wt% or more based on 100 parts by weight of the mixed composition of the whole serum type cosmetics.

In the present invention, Zygo extract is extracted from the root of Zygo pollack which belongs to Lily family. Boripyrin, which is a component derived from Zygo extract, is excellent in stability and has a cell active component and is excellent in skin elasticity enhancement effect. It is known to be used as a material. Specifically, borphyrin is mainly distributed in the dermis of the skin, and is known to promote the growth of fibroblasts that affect the moisturization of collagen, elastin, and skin elasticity and wrinkle formation, thereby greatly enhancing skin elasticity . In addition, it is known that the boripyrin contained in the gum extract has excellent whitening effect and skin regeneration effect.

In the present invention, the gold nest extract contains a large amount of EGF (Epidermal Growth Factor), FGF (fibroblast growth factor) and sialic acid, and these components act inside the skin of the skin, Promoting the generation of cells related to skin elasticity enhancement, thereby improving elasticity, improving wrinkles and skin lifting effect, promoting the production of hyaluronic acid, which is known as a skin moisturizing factor, and bringing about skin moisturizing and whitening.

In the present invention, the peptides may be at least one selected from the group consisting of caprate tripeptide-1, aldehyde-oligopeptide-1, S-oligopeptide-1 and acetylhexapeptide-8, , Elasticity enhancement and skin lifting effect. Specifically, copper tripeptide-1 has been reported as a functional substance that strengthens skin barrier and improves skin elasticity and improves wrinkles. Acetyl hexapeptide-8 is known as a skin- It is a naturally occurring non-irritating, non-toxic ingredient and it is known as a functional material that can enhance skin elasticity and alleviate wrinkles. In particular, acetylhexapeptide-8 is also known as "botox peptide", which is known to temporarily inhibit aging by participating in controlling the intensity of facial muscle contraction. In addition, the rh-oligopeptide-1 consists of 53 amino acids and is a protein involved in the improvement of skin damage. EGF is known to stimulate epithelial cells to induce skin activation. Quot; or " Human Oligopeptide-1 ". In addition, SIE oligopeptide-1 is a component that decreases as the skin ages, and it strengthens the skin barrier, thereby giving elasticity to the wrinkles caused by aging of the skin, thereby bringing about the effect of improving wrinkles. .

In the present invention, niacinamide is a complex of water-soluble vitamin B, which is also called nicotinic acid and niacin. Melanocytes, which are skin-inducing components of melanin, are produced by tyrosinase in the basal layer, which is the lowest layer of the epidermal layer. To move to the stratum corneum close to the surface of the skin and cause pigmentation. Niacinamide can inhibit pigmentation by inhibiting this movement, and can lighten the pigment deposited on the stratum corneum to make skin whitening effect. In addition, niacinamide may contribute to the prevention of moisture loss in the skin by increasing the synthesis of intercellular quality. In addition, it has an antioxidant effect that suppresses active oxygen which is a cause of skin aging, and also has an effect of improving elasticity and improving wrinkles in addition to whitening function. In order for the niacinamide to exhibit the above-mentioned effect, it should be contained in an amount of 2 wt% or more based on 100 parts by weight of the mixed composition of the whole serum-type cosmetics. However, it is known that when the blending amount of niacinamide is excessive, it causes snow flakes. For this reason, it may be preferable that 2 to 4 wt% of niacinamide is included.

In the present invention, the Cryptomeria japonica extract is a component extracted from the genus Chrysanthemum belonging to the soybean family as early as the beginning of the month of the herb, which is also called as a herb extract, a safflower seed, a crusher, Cole seeds are known to have effects such as topical and anti-inflammatory effects. The outpost is a medicinal plant rich in resources used to treat skin diseases such as trauma bleeding and shingles. In addition, the outpost of the wooly owl contains trigonelline and canavanine, and the seed contains canaline, canavanine, homoserin, coumestrol, and the like . In addition, the outpast of Cubuntus is called Safflower seed, the seed is Cinnabon seed and it is used for medicinal purposes. The Safflower straw is collected from March to April and contains trigonelline, choline and adenine, , And seeds such as cannarin, canavanine, and homoserine are known to have an effect of helping blood circulation and brightening the eyes. The present inventors confirmed the skin aging inhibition, whitening effect, antioxidative effect and the like of the perilla leaf extract and applied it as a raw material of the multi-functional serum through the skin physiological activity test of the perilla seed extract.

In the present invention, Galactomyces fermentation filtrate is a natural yeast fermentation filtrate component. Galactomyces is one of the natural yeasts used in wine production in a brewery, and contains galactomose fermented filtrate rich in vitamins, minerals, organic acids , Enzymes, yeast peptides and other ingredients to help skin elasticity. It also helps to keep skin tone clear and transparent by organizing keratin and skin texture.

In the present invention, chaga mushroom extract is a component extracted from chaga mushroom which is parasitic on birch trees of more than 45 degrees north such as Siberia, North America and northern Europe. It is a kind of enzyme which decomposes elastin, which is a protein soluble enzyme that degrades elastin, It is known that it inhibits the activity of MMP and improves skin texture. In addition, chaga mushroom extract contains an excess of ingredient called LS8865 which is excellent at moisturizing and at the same time, whitening and shrinking action of pores, and can bring about skin whitening, moisturizing and pore shrinking effect.

In the present invention, the bifidobacteriaceae is a component obtained by dissolving a fermentation product of bifidobacteria. Bifidobacterium is a lactic acid bacteria closely related to human health and has immune and anti-cancer effect. Bifidobacterial effluent is fermented by first and second culture of bifidobacteria to dissolve, ferment and filter It is fermented seafood which is produced and is very difficult to extract and has extremely low extraction. Protein, lactose, vitamins and other minerals contained in Bifidobacteria spp. Are beneficial to skin vitality and can help to reduce skin hypersensitivity by participating in improving skin immunity.

In the present invention, ferulic acid is a component obtained from seed coat of rice (oriza sativa linne (gramincae)) and rice embryo, and is mainly an ester component of triterpene alcohol and ferulic acid. It can prevent skin oxidative stress from free radicals (free radicals) which cause skin aging and can help skin vitality with plant active ingredients. It also promotes circulation in the skin, helps improve sebum secretion, and increases skin activity due to aging.

In the present invention, the mixed water of golden, yellow, white, mung bean and licorice extracts has antibacterial and anti-inflammatory action as main functions, but is not limited thereto and has functions of whitening, elasticity enhancement, antioxidation and wrinkle improvement. Hereinafter, the efficacy and effects of the respective constituent materials constituting the mixed-extracted water will be described in detail.

In the present invention, gold is a medicinal plant, and its roots are used as one of herbicides, diuretic, branch, insect and anti-inflammatory agents. The golden extract is extracted from the root of gold and can act as a anti-inflammatory, antibacterial, anti-allergic, antioxidant, whitening and moisturizing function and can be used as a raw material for skin cosmetics. In the present invention, Rhizoma belongs to the perennial plant and is known to contain active ingredients such as berberine, coptisine, worenine and palmatine.

The extract of Huangryun extract is extracted from the roots of Huangryun. It suppresses the activity to prevent the external harmful substances from proliferating on the skin surface, suppresses the hypersensitivity reaction of the skin caused by the harmful substances, and protects against the deterioration and decay of cosmetic products Can be used as an antiseptic.

The extract is extracted from the bark of an acacia tree. It contains berberine, which is an alkaloid. It inhibits the activity and growth of substances harmful to the skin to help skin care, To protect the skin.

The mung bean extract can be obtained by steam distillation of mung bean seeds. It contains palmic acid, stearic acid, linoleic acid, vitamins, flavonoids and saponin components. In addition to antibacterial and anti-inflammatory actions, And it is known to have the effect of improving elasticity.

The licorice extract inhibits the substances that are harmful to the skin from exhibiting activity, inhibits the hypersensitivity reaction of the skin, suppresses the oxidation reaction of the skin caused by the active oxygen which is the cause of skin aging, and contributes to enhance the elasticity of the skin.

In the present invention, the mixed extract of Hwangchilji leaf, Baeksoo, Geumo, and germanium powder has the effects of antioxidation, antibacterial, anti-inflammation, elasticity enhancement, pore reduction, whitening and the like. Hereinafter, the specific efficacy and effects of each composition material constituting the mixed extract will be described.

In the present invention, the extract of Huangchil leaf is a component extracted from the leaves and stems of Huangchu tree belonging to the evergreen tree of Phalaenopsis phalaenopsis, and it is known as a medicinal plant having excellent antibacterial and anti-inflammatory action. It can improve the elasticity and moisturizing effect, and can inhibit the activity of tyrosinase that produces melanin, which can help skin whitening.

In the present invention, white water is one of the medicinal plants that have been cultivated for a long time as a vine plant belonging to a vine perennial herbaceous plant, whose roots extend long to the side and have round root roots. In addition to antioxidant, antibacterial and anti-inflammatory functions, the components extracted from the roots of Bacillus subtilis can enhance the skin's immunity and contribute to the improvement of elasticity, thereby improving wrinkles and whitening.

The germanium powder used in the present invention is harmless to the human body when it is used in an appropriate amount and is known to promote physiological activity such as promoting oxygen supply in the human body cells. The germanium powder adsorbs wastes containing sebum in the skin surface and pores, And a small amount of germanium absorbed in the skin can bring about an effect of improving the elasticity and wrinkling of the skin by the activating enzyme.

In the present invention, the aloe vera leaf extract contains about 300 various ingredients such as vitamins A, C, D, E, B12, amino acids and aloesin, and softens, And manage it. In addition, it contains a lot of moisture, is involved in maintaining the moisture of the skin, and can calm the sensitive and sensitive skin.

In the present invention, the panthenol is absorbed into the skin and can be transformed into a substance called pantothenic acid (vitamin B5), and has an effect of binding moisture, thereby obtaining the same effect as a moisturizer. In addition, it is possible to supply the deficient water directly, to activate the moisturizing function, to maintain the skin moisturizing effect, to provide shine to the skin, to prevent moisture evaporation, and to maintain the moisture of the skin itself.

In the present invention, sodium hyaluronate may include a sodium salt of hyaluronic acid (hyaluronic acid), and may be a harmless ingredient to the human body as a natural moisturizing factor present in a certain amount on the skin. Its own moisture content reaches 6000 times of the normal moisture factor. It absorbs and stores the surrounding water. It is dissolved in water as white powder and it can control the viscosity depending on the molecular weight and concentration of hyaluronic acid, PH, and ionic strength. It is a natural moisturizing ingredient that provides moisture to the skin to keep it from drying out. When it is contained in cosmetics, it attracts moisture around the skin to prevent water evaporation. It produces a thin and transparent moisturizing film to reduce external stimuli Lt; / RTI >

In the present invention, the polyquaternium-51 may be a polymer material called a repeater (PMB), and may be a component having a moisturizing power. It has a chemical structure similar to the protein forming the cell membrane and can be obtained by culturing a phosphate lipid polar group. May be a polymer having excellent biocompatibility and high hydrophilicity. In addition, when used in cosmetics, it forms a thin film on the skin, and acts as a high moisturizing agent for suppressing water evaporation in the skin, thereby maintaining a moist skin constantly.

Hereinafter, the cosmetic composition to be blended together with the active ingredient will be described.

In one embodiment of the present invention, the mixed composition of multi-functional serum-type cosmetics may include at least one selected from heptyl undecylenate and caprylic / capric triglyceride as the skin softening agent.

In one embodiment of the present invention, the mixed composition of multi-functional serum-type cosmetics may include at least one selected from the group consisting of gradually increasing acrylate / C10-30 alkyl acrylate crosspolymer, xanthan gum, and carbomer.

In one embodiment of the present invention, the mixed composition of multi-functional serum-type cosmetics may contain at least one selected from polysorbate 80 and olive oil phage-7 ester as the solubilizing agent.

In one embodiment of the present invention, the mixed composition of multi-functional serum-type cosmetics may contain at least one selected from preservatives, sequestering agents, pH adjusters and fragrances as additives.

The present invention provides a method for producing a mixed composition of a multi-functional serum-type cosmetic composition comprising the composition as described above.

In an embodiment of the present invention, the multifunctional serum-type cosmetic composition may be prepared by mixing raw materials such as gold, yellow radish, yellow white, mung bean, licorice mixed extract water, niacinamide, acrylate / C10-30 alkyl acrylate crosspolymer, Gum, aloe vera leaf extract, carbomer, polyquaternium-51, gold nest extract, disodium edithia, ferulic acid, chaga mushroom extract, galactomyces fermented filtrate, bifida efficacy seafood and sequestering agent , Olive oil phage-7 ester, olive oil extract, caprylic / capric triglyceride, heptyl undecylenate To prepare an oily mixture, the oily mixture from the second stage is added to the oily mixture of the first stage, stirred at 70 to 90 DEG C, A fourth step of lowering the temperature of the aqueous mixture to 20 to 40 캜 and adding panthenol, sodium hyaluronate, peptides, adenosine and additives, stirring and mixing, .

More preferably, the mixed composition of multifunctional serum-type cosmetics comprises 0.04 to 0.5 wt% of adenosine, 0.5 to 2.5 wt% of Zygo extract, 2 to 7 wt% of peptides, 2 to 45 wt% of niacinamide, 0.5 to 3 wt% , 0.5 to 2 wt% of Celton's extract, 0.5 to 3.5 wt% of Galactomyces fermented filtrate, 0.5 to 4 wt% of chaga mushroom extract, 3 to 5.5 wt% of mixed extracts of golden radish, , 0.5 to 2 wt% of bifidobacteria flavor, 3 to 5.5 wt% of aloe vera leaf extract, 0.5 to 2 wt% of panthenol, 0.4 to 1.5 wt% of sodium hyaluronate, 0.5 to 1.5 wt% of polyquaternium- % Of the skin-softening agent, 2 to 10 wt% of the skin-softening agent, 5 to 15 wt% of the increasing agent, 2 to 10 wt% of the solubilizing agent, 2 to 15 wt% of the additive and 100 wt% , ≪ / RTI > Hereinafter, a preparation example of a mixed composition of a multi-functional serum-type cosmetic composition according to the present invention will be described in detail.

Hereinafter, examples and experimental examples of the present invention will be described.

[ Experimental Example  1] Coleoptera seed extract Skin physiological activity  Experiment

1. Measurement of cytotoxicity and survival rate by MTT assay

Cell viability was measured by the method of Carmichael et al. (Cancer Res 1987; 47 (4): 936-942). Raw 264.7 mouse macrophage cells were cultured in DMEM medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% streptomycin under 5% carbon dioxide at 37 ° C. Cells were plated in 96-well plates at a concentration of 1 × 10 ⁴ cells / well in an amount of 100 μl each. After culturing in an incubator for 24 hours, each extract was treated with 10 μl of each concentration and cultured for another 24 hours. MTT solution was added to each well and reacted for another 4 hours. Then, the supernatant was removed to remove the formazan formed on the bottom of the well, and 100 μl of dimethylsulfoxide was added. After 30 minutes, the absorbance at 540 nm (Power Wave X340, BIO-TEK). The average value was obtained by repeating the experiment six times for each concentration of 12.5 mg / ml, 25 mg / ml, and 50 mg / ml of Coleoptera seed extract. The relative cell viability was determined by taking the absorbance of the control group without the extract as 100% Respectively.

The toxicity of Coleus koreanum Seed Extract to Raw 264.7 cells was measured by MTT analysis and found that 91.54 ± 6.24%, 103.00 ± 5.22%, and 96.61 ± 7.56% of the extracts were obtained at 12.5 mg / ㎖ and 25 mg / Cell survival rate.

As a result of the MMT analysis, 103% cell proliferation effect was observed at a concentration of 25 mg / ml of the extract, and 96.61% cell survival rate even at a concentration of 50 mg / ml. These values are shown in the following table: Cell survival rate of snake strawberry fruit is 50-80%, cell survival rate is about 80% at 500ppm of ethanol and acetone extract, and 60% of cell survival rate at 500ppm concentration of water extract and vitamin C. , It can be concluded that the extract of Coleoptera japonica has little effect on cytotoxicity.

2. The DPPH radical Scatters  Measure

DPPH radical scavenging activity was examined to investigate the antioxidative effect of the extract of Coleoptera syrup. Skin is aged by physiological aging and photoaging, and free radicals, which are active oxygen species in the cells, cause deformation of biomolecules, leading to aging. Therefore, in order to delay skin aging, an ability to erase free radicals generated in the body is required.

The radical scavenging activity of the black currant seed extract was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl, Sigma) as the method of Blois (Nature 1958; 26: 1199-1120). 10 μl of the sample solution dissolved in methanol and 190 μl of the DPPH solution (300 μM) were mixed and allowed to stand in a dark room for 15 minutes, and the absorbance (Power Wave X340, BIO-TEK) was measured at 517 nm. The experiment was repeated six times at concentrations of 2.5 mg / ml, 5 mg / ml and 10 mg / ml in the control and experimental groups, respectively. The scavenging activity was calculated on the basis of the absorbance of the reaction solution mixed with 10 μl of methanol instead of the sample solution, and ascorbic acid, which is an antioxidant in the body, was used as a positive control. The DPPH radical scavenging ability of the perilla seed extract was 63.52 ± 2.86% at 2.5 mg / ml, 90.71 ± 0.65% at 5.0 mg / ml and 91.6 ± 0.21% at 10.0 mg / ml, In the case of acetic acid, DPPH radical scavenging activity was 93.56 ± 0.07% at 5.0 mg / ml, 93.72 ± 0.04% at 5.0 mg / ml and 93.71 ± 0.05% at 10.0 mg / And it was confirmed that it is similar to corbic acid. There was statistically significant difference between scavenging ability and ascorbic acid scavenging ability at each concentration (p <0.05).

In addition, DPPH radical scavenging activity was found to have a high concentration-dependent effect on the extract of Coleoptera sieboldii. In particular, it exhibited a DPPH radical scavenging activity of 90% or more at a concentration of 5.0 to 10.0 mg / ml, and this value shows almost similar antioxidative effects as DPPH radical scavenging activity of ascorbic acid as a control group.

3. Measurement of Elastase Inhibitory Activity of Curd Leaf Extract

Elastase inhibitory activity was measured by the method of Cannell et al. (Plaanta Med 1988; 54 (1): 10-14). Human leukocyte elastase was dissolved in 50 mM sodium acetate buffer solution (pH 5.3) to prepare 1 unit / ml solution. Para-nitroanilide was dissolved in dimethyl sulfoxide to prepare a 20 mM stock solution. 20 μl of elastase and 20 μl of the sample were dispensed into a 48-well plate, and the plate was washed with 10 mM sodium phosphate buffer pH 6.8) was added 200 μl of 400 μM para-nitroanilide. The reaction solution was incubated at 37 ° C for 20 minutes, and 120 μl of the reaction solution was transferred to a 96-well plate and the absorbance (Power Wave X340, BIO-TEK) was measured at 410 nm. Repeated experiments were repeated 5 times at concentrations of 25 ㎍ / ㎖, 50 ㎍ / ㎖, 100 ㎍ / ㎖ and 250 ㎍ / ㎖ in the control and experimental groups, respectively. Quercetin was used as a positive control, and the inhibitory activity of elastase was calculated according to the following formula (1).

[Equation 1]

Inhibitory activity (%) of elastase = {1- (S-B) / C} * 100

Where B is the absorbance of the reaction solution to which 10 mM sodium phosphate buffer solution (pH 6.8) is added instead of the enzyme, and C is the absorbance of the reaction solution containing 10 mM sodium phosphate buffer (pH 6.8 ) Is added to the reaction solution.

The elasthase inhibitory activity of the sesame seed extract was 73.33 ± 1.58% at 25 μg / ml, 80.25 ± 2.07% at 50 μg / ml, 90.82 ± 2.46% at 100 μg / ml, 97.36 ± 1.69% at 250 μg / The activity of quercetin in the control group was 75.60 ± 1.69% at 25 μg / ml, 79.12 ± 1.03% at 50 μg / ml, 85.03 ± 1.03% at 100 μg / ml and 105.28 ± 3.63% at 250 μg / Lt; / RTI &gt;

Elastase is an enzyme that degrades elastin, and by this action it is known to be the main enzyme for reducing elasticity and wrinkling by cutting the network structure of the dermis. As a result of the measurement of the inhibitory activity of the elastase, the inhibitory activity was 90.82% at 100 μg / ml of the curd seed extract, and this value was 100 μg / ml as compared with quercetin, a flavonoid-based elastase inhibitor, (P <0.05). The inhibition of elastase activity of Coleoptera seed extracts was significantly higher than that of the control group (p <0.05).

4. Measurement of inhibitory activity of MMP-1 (collagenase)

It is known that when the content of collagen is reduced by the increase of collagen decomposition by the expression of MMP such as collagen (collagen) degrading enzyme by ultraviolet rays, skin elasticity is reduced and wrinkles appear. The collagenase inhibitory activity test is for measuring the degree of reduction of skin aging by measuring the inhibitory activity of collagenase which hydrolyzes collagen which plays a supporting role of skin.

MMP-1 inhibitory activity was measured by the method of Sim et al. (J Soc Comet Scientists 2004; 30: 439-444). 300 콜 of collagen solution (0.25 mg / ml) dissolved in PBS (pH 6.0), 600 시 of sample and 600 콜 of collagenase solution (0.5 unit) were added. After 20 minutes at room temperature, the fluorescence value was measured using a fluorescence spectrophotometer (Hitachi) at an absorption wavelength of 280 nm and an emission wavelength of 300 nm. In the control group, PBS (Phosphate Buffer Saline) was added in the same amount instead of the sample solution. The mean values were used for each of the concentrations of 50 ㎍ / ㎖, 100 ㎍ / ㎖, 250 ㎍ / ㎖ and 500 ㎍ / ㎖ in the control and experimental groups, respectively. The quercetin inhibiting activity of collagen denaturation The inhibitory activity of collagenase was calculated according to the following formula (2).

&Quot; (2) &quot;

Inhibitory activity of collagenase (%) = {(C-S) / C} * 100

Where S is the absorbance of the sample and enzyme-containing reaction solution, and C is the fluorescence of the reaction solution to which PBS is added in place of the sample.

The inhibitory activity of MMP-1 on CMP was 23.00 ± 0.06% at 50 μg / ml, 43.9 ± 0.06% at 100 μg / ml, 76.9 ± 0.02% at 500 μg / ml at 250 μg / The inhibitory activity of quercetin was 95.24 ± 0.03%, and that of the control group was 69.95 ± 0.12% at 100 μg / ml, 84.2 ± 0.09% at 100 μg / ml, 98.4 ± 0.02% at 500 μg / 99.95 ± 0.01%, respectively, and the collagenase inhibitory activity of the vernal seed extracts was significantly lower than that of the control group, quercetin (p <0.05).

However, the MMP-1 inhibitory activity of Cuneiform Seed Extract showed a high concentration-dependent effect, and in comparison with Coenzyme Q 10, which is effective for prevention of skin aging and cell regeneration, showed inhibitory activity of 1% to 91.1% The extract of Perilla seeds showed 95.24% at 500 ㎍ / ㎖, suggesting that the inhibitory activity of MMP-1 is superior.

5. Measurement of inhibitory activity of tyrosinase of Coleoptera seed extract

The inhibition activity of tyrosinase was examined by measuring the enzyme inhibitory activity of tyrosinase, which is involved in the production of melanin, which is a cause of abnormal pigment formation, and examines the whitening effect of the black currant seed extract.

Tyrosinase inhibitory activity was measured using the method of Mason et al. (Biochim. Biophys Acta 1965; 111: 134-146). 220 μl of a 0.1 M phosphate buffer solution (pH 6.5), and 20 μl of a sample dissolved in a 0.1 M phosphate buffer solution (pH 6.5) were mixed. 20 mu l of mushroom tyrosinase (1,000 units / ml) was dispensed into the mixed solution, and the mixture was reacted at 37 DEG C for 30 minutes. The amount of dopachrome produced by the reaction was calculated by measuring absorbance (Power Wave X340, BIO-TEK) at 490 nm. Repeated experiments were repeated three times at concentrations of 6.25 mg / ml, 12.5 mg / ml, 25 mg / ml, and 50 mg / ml in the control and experimental groups, and the average value was used to inhibit melanin production. Reducing ascorbic acid was used as a positive control, and the inhibitory activity of tyrosinase was calculated according to the following equation (3).

&Quot; (3) &quot;

Inhibitory activity (%) of tyrosinase = {(C-S) / C} * 100

Where C is the absorbance of the reaction solution to which PBS is added instead of the sample, and S is the absorbance of the reaction solution containing the sample.

The tyrosinase inhibitory activity of Coleoptera seed extract was activated in a concentration dependent manner. The inhibitory activity was 61.47 ± 2.85% at 6.25 mg / ml, 73.65 ± 1.47% at 12.5 mg / ml, 89.41 ± 5.77% at 25 mg / ml and 94.41 ± 9.15% at 50 mg / The inhibitory activity of the free acids was 68.81 ± 0.29% at 6.25 mg / ㎖, 88.75 ± 1.58% at 12.5 mg / ㎖, 90.18 ± 0.44% at 25 mg / ml and 89.99 ± 0.29% at 50 mg / ml. It was significantly lower at 6.25 mg / ㎖ and 12.5 mg / ㎖, but higher at 50 ㎎ / ㎖ (p <0.05) than the control group of ascorbic acid, which is a representative whitening substance.

[ Example  One]

A multi-functional serum-type cosmetic having the composition shown in Table 1 below was prepared. Specifically, the ingredients shown in Table 1 below are divided into oil, water, and water, weighed, mixed and prepared. First, the aqueous phase mixture was heated to 80 DEG C and then the oil phase mixture was mixed with the aqueous phase mixture and stirred sufficiently. The temperature of the oil phase mixture was lowered to 40 DEG C, and the mixture was stirred. Was prepared.

Ingredients function Content [wt%] Classification of raw materials Purified water solvent 40.130







Awards
Golden, Yellow, White, Mung bean, Licorice Antioxidant, anti-bacterial, anti-inflammatory 5.000 Niacinamide Whitening 3.000 1,2-hexanediol Preservative 1,000 Acrylate / C10-30 alkyl acrylate crosspolymer Incrementer 0.300 Xanthan gum Incrementer 1,000 Aloe vera leaf extract Antibacterial, anti-inflammatory, regenerating, moisturizing 5.000 Carbomer Incrementer 10,000 Polyquaternium-51 Film forming agent, moisturizer 1,000 Goldsmith's nest extract Wrinkles, whitening, regeneration 2.000 Dissodium dithiene Preservative (metal ion sequestrant) 2.500 Ferulic acid, ethoxydiglycol Antioxidant, aging delay 1,000 Mushroom extract Reduce pores, improve carcass 3.000 Galactomisses fermentation filtrate Regeneration, whitening, antioxidant 2.500 Bifidobacteria Regeneration, whitening, antioxidant 2.500 Polysorbate 80 Solubilizing agent 4.000


Paid
Gum extract Elasticity, whitening, wrinkle improvement 2.000 Olive oil phage-7 ester Solubilizing agent 0.800 Chestnut extract Whitening, anti-aging, wrinkle improvement 1,000 Caprylic / capric triglyceride Skin softening agent 2.900 Heptyl undecylate Skin softening agent 0.300 Triethanolamine pH adjusting agent 0.200





injury



Panthenol Antimicrobial, anti-inflammatory 1.500 Sodium hyaluronate Moisturizing, regenerating 1,000 Copper tripeptide-1 Regeneration, tinnitus, moisturizing, wrinkles 2.000 Al-H-oligopeptide-1 Reducing pores, moisturizing, improving wrinkles, whitening 1,000 Acetyl hexapeptide-8 Wrinkle improvement, skin texture improvement 2.000 SH-Human Oligopeptide-1 Wrinkles, elasticity improvement, whitening 1,000 Adenosine wrinkle improvement 0.040 Ethylhexyl Glycerin Preservative 0.050 1,2-hexanediol Preservative 1,000 Spices 0.36 Sum 100

[ Comparative Example  One]

A mixed composition of the serum form was prepared in the same manner using the same ingredients as in Example 1, except that the functional material was not included.

[ Experimental Example  2]

A stability test of the mixed composition of the serum formulations prepared according to Example 1 was performed. The stability test was carried out three times in total (immediately after completion of the serum preparation, after three days of aging, and after seven days of aging), and the stability of the pH measurement and formulation was evaluated by observing the naked eye and smell.

In order to protect the skin from pathogens, the acidity of human skin should be maintained at a pH of 4 to 6. In order to maintain such acidity, it is preferable to use neutral or slightly acidic cosmetics. For this reason, the pH was measured three times. As a result, it was confirmed that the average pH was maintained at 5.5.

 Further, as a result of observing the stability of the formulation, it was confirmed that no remarkable alteration occurred in the separation phenomenon of the oil phase and the water phase, the color, and the like.

[ Experimental Example  3]

To examine the effect of the serum type cosmetic prepared according to Example 1 on the skin, a skin stimulation patch test was carried out as follows.

A group consisting of 10 subjects (4 males and 6 females) in their 20s and 40s who had no skin disease or allergy was subjected to a patch test on the medial side of the upper abdomen. After disinfecting the patch area, the pin chamber to which the serum type cosmetic composition according to Example 1 was added was attached to the test area. After 24 hours, the patches were removed, and after 1 hour, the skin reaction of the test site was read based on the skin irritation criteria shown in Table 2 below. The results read by the judgment criteria are shown in Table 3.

Separator Criteria - No stimulation ± Minor erythema + Mild erythema, rash and papules occur ++ Erythema, papules and blisters +++ Occurrence of blisters

As shown in Table 5 below, all of the subjects were found not to have allergies or other skin irritation.

sign - ± + ++ +++ Results (n = 10) 10 - - - -

[ Experimental Example  4]

The following clinical tests were conducted to examine the effect of the serum type cosmetic prepared according to Example 1 on the skin regularly after regularly applying to the skin.

1. Selection and allocation of research subjects

The researchers explained the purpose and method of the clinical trial to the female students of K university located in Gwangju Metropolitan City and explained the condition that the clinical trial was limited to the same grade student with no specific disease and good health condition. A total of 30 female students who agreed to participate in clinical trials were selected after completing the self - filling questionnaire. A total of 34 patients were randomly assigned to the control group and the test group in the order of the test group, except for the questionnaire in which the response contents were unclear or insufficient. However, except for 4 patients who withdrew from clinical trials due to individual reasons, the final 30 patients were selected and 15 patients were assigned to each clinical trial.

2. Collection of survey data

The questionnaire consisted of 6 items. In the skin care clinical trials, it is composed of contents that can affect the skin condition, age, recognition of health condition, average sleeping time per day, frequency of regular exercise, daily water intake, number of meals per day Respectively.

3. Application of Serum Formulation Mixture Composition

In order to evaluate the effect of the serum-type cosmetics prepared according to Example 1 on the skin care, the serum-type cosmetics of Example 1 were regularly applied to the face of 15 test groups. Serum type cosmetics were applied for 8 weeks after washing in morning and evening. In addition, in the control group, the serum of Comparative Example 1 was applied by the same cycle and method as those of the test group for 8 weeks.

4. How to measure skin beauty effect

The water point, the elasticity index, the pigment index, the roughness index, and the pore size before and 8 weeks after the clinical test were measured using the Aramo TS Diagnosis System of Aramu Hubis in non-invasive manner Respectively.

4-1 Measuring Moisture Index

The moisture content of the skin surface is measured and quantified by the ability of storing the electric charge. The principle of measuring the conductivity and the load between the probes directly contacting the skin is measured. When the measured value is within the range of 1 to 30, the moisture is very low, when the range is within the range of 31 to 40, the moisture is insufficient, and when the moisture content of the skin stratum corneum is higher, When it is within the range of 41 to 100, it indicates good water, and the average standard value of the twenties is the water index 37 arbitrary unit.

4-2 Measurement of elasticity index

The principle of elasticity index measurement measures skin change and restorative power according to the duration of suction and inhalation time as basic principle. Minimizing pressure of 300mmbar is applied inside the device to quickly and uniformly inhale the skin, The system recognizes and changes the value. The elasticity index is within a range of 0 to 100, a range of 0 to 30 is low, a range of 30 to 70 is usually a range of 70 to 100, and a standard value of 20 is a elasticity index 68 UR / Uf to be.

4-3 Measurement of pigment index

The MI (melanin index) represents the index of the reflected light of the reflectance spectrophotometer by the optical measuring technique, and the MI value in the range of 20 to 25 in the range of 1 to 100 is 25 MI.

4-4 Measurement of roughness index

The roughness index is an R-value obtained by analyzing the digital image measured by an image analyzer. The R-value is within a range of 1 to 100, and the roughness standard value of 20 is 50R-value.

4-5 Measurement of pore size

The pore size was measured by analyzing the digital image measured by an image analyzer.

4-5 Statistics Processing Method

In the clinical trial effect test for skin beauty, the difference between the control and the test group was analyzed by the variance analysis and the chi-square test. The degree of change in the skin condition before and after the test was used in the analysis of variance (ANOVA), the statistical procedure used to analyze the mean difference between groups or conditions, and the method of verifying whether the population represented by each condition level is the same Duncan test was used for the post - test to see differences between groups. Statistical significance was p <0.05.

4-6 Clinical trial results

The results of the questionnaire and statistical analysis showed that the mean age, health status, daily average sleep time, frequency of regular exercise, daily water intake, . (p < 0.05)

The control group was divided into the following before and after the use of the serum-forming composition prepared according to Comparative Example 1, and the following indices were measured: before and after using the serum-type cosmetic prepared according to Example 1, Each index was measured, and the measurement results are shown in Table 4 below. (Values obtained from the measurements are expressed as mean ± standard deviation.)

Item Control group (n = 15) The test group (n = 15) Moisture index I'm 47.38 + - 5.72 47.19 + - 4.40 after 45.35 + - 6.40 51.02 + - 5.86 Elasticity index I'm 57.07 + - 8.30 58.40 + - 8.95 after 54.17 + - 8.23 63.00 + - 9.69 Pigment index I'm 49.92 + - 14.86 48.51 + - 13.82 after 55.33 + - 15.57 25.15 + - 12.44 asperity
Indices I'm 24.08 + - 3.43 26.65 + 2.84 after 28.13 + - 3.64 23.32 ± 3.79 Pore size I'm - 81.20 ± 13.78 after - 77.80 ± 9.75

As shown in Table 4, the moisture index and the elasticity index of the control group decreased after the use of the serum, while the water index and the elasticity index after the serum type cosmetic use according to Example 1 of the test group decreased Respectively. Further, referring to Table 4, it can be seen that the dye index and the roughness index of the control group increased while the serum index increased, while the dye index and the roughness index of the test group decreased after use of the serum-type cosmetics. Further, it was confirmed that the pore size decreased after the use of the serum-type cosmetic preparation of Example 1 in terms of pore size.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (7)

In a mixed composition of a serum-type cosmetic,
Adenosine; Gum extract; Peptides; Niacinamide; Gold nest extract; Coleoptera seed extract; Galactomyces fermentation filtrate; Mushroom extract; Golden radish, yellow, white, mung bean, licorice mixed extracts; Ferulic acid; Bifidobacteriaceae; Aloe vera leaf extract; Panthenol; Sodium hyaluronate; Polyquaternium-51; As an active ingredient,
Purified water is contained so that the active ingredient is 14.5 to 48 wt%, the skin softening agent is 2 to 10 wt%, the increasing 5 to 15 wt%, the solubilizing agent is 2 to 10 wt%, the additive is 2 to 15 wt%, and the total mixed composition is 100 wt% Wherein the adenosine is contained in an amount of 0.04 wt% or more, and the niacinamide is contained in an amount of 2 to 4 wt% with respect to the mixed composition.
The method according to claim 1,
Characterized in that said peptides include at least one selected from the group consisting of caprate peptide-1, al-H-oligopeptide-1, acetyl hexapeptide-8 and S-human oligopeptide-1. Mixed composition.
The method according to claim 1,
Wherein the skin-softening agent comprises at least one member selected from heptyl undecylenate and caprylic / capric triglyceride.
The method according to claim 1,
Wherein the thickener comprises at least one selected from acrylate / C10-30 alkyl acrylate crosspolymer, xanthan gum, and carbomer.
The method according to claim 1,
Wherein the solubilizing agent comprises at least one selected from the group consisting of polysorbate 80 and olive oil pigment-7 ester.
The method according to claim 1,
Wherein the additive comprises at least one selected from the group consisting of a preservative, a sequestering agent, a pH adjusting agent and a fragrance.
A method for producing a multifunctional serum-type cosmetic mixed composition according to any one of claims 1 to 6,
The present invention relates to a cosmetic composition for a multi-functional serum-type cosmetic composition, which comprises a raw material selected from the group consisting of golden, yellow, white, mung bean, licorice mixed extract, niacinamide, acrylate / C10-30 alkyl acrylate crosspolymer, xanthan gum, A water mixture is prepared by mixing carborum, polyquaternium-51, gold nest extract, disodium edithia, ferulic acid, chaga mushroom extract, galactomisse fermented filtrate, bifidobacteriaceae and metal ion sequestering agent ;
Liposomes, polysorbate 80, olive oil phage-7 ester, enamel seed Extract, caprylic / capric triglyceride, and heptyl undecylenate to prepare an oil-in-water mixture;
A third step of adding an oil-in-water mixture from the second step to the water-based mixture in the first step and mixing with stirring at 70 to 90 ° C to prepare an oil-water mixture; And
A fourth step of lowering the temperature of the water-liquid mixture to 20 to 40 캜, adding panthenol, sodium hyaluronate, peptides, adenosine and an additive, and stirring and mixing;
And purified water is added so that the active ingredient is 14.5 to 48 wt%, the skin softening agent is 2 to 10 wt%, the increasing 5 to 15 wt%, the solubilizing agent is 2 to 10 wt%, the additive is 2 to 15 wt% Wherein the adenosine is contained in an amount of 0.04 wt% or more, and the niacinamide is contained in an amount of 2 to 4 wt% with respect to the total mixed composition.