JP2008174459A - Anti-aging agent, external preparation for skin and food and drink for beauty - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a highly safe and inexpensive anti-aging agent derived from natural products and having high productivity and excellent I type collagen production promoting actions, fibroblast proliferating actions or laminin 5 production promoting actions though daily usable. <P>SOLUTION: The anti-aging agent is characterized by comprising at least any of Gynura bicolor DC. and an extract thereof as an active ingredient. The extract of the Gynura bicolor DC. is preferably an extract with a mixed solvent of water and ethanol. The type I collagen production promoter is characterized by comprising at least any of the Gynura bicolor DC. and the extract thereof as the active ingredient. The fibroblast proliferation agent is characterized by comprising at least any of the Gynura bicolor DC. and the extract thereof as the active ingredient. The laminin 5 production promoter is characterized by comprising at least any of the Gynura bicolor DC. and the extract thereof as the active ingredient. An external preparation for skin comprises the anti-aging agent and a food and drink for beauty comprises the anti-aging agent. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

[Technical Field] The present invention relates to an anti-aging agent containing an extract of Gynura bicolor DC . : Kinkisou, and an external skin preparation and cosmetic food and drink containing the same.

The skin is composed of the epidermis, basement membrane, and dermis. The dermis is composed of fibroblasts and extracellular matrices such as collagen (collagenous fibers), elastin (elastic fibers), and hyaluronic acid that are secreted from the cells. The basement membrane is present at the boundary between the epidermis and the dermis and plays an important role not only in connecting the epidermis and the dermis but also in maintaining the skin function (see Non-Patent Document 1).
In young skin, the proliferation of fibroblasts is active, and the interaction between the skin tissues such as fibroblasts and collagen remains homeostatic, and the interaction between the epidermis and dermis by the action of the basement membrane By maintaining the constancy, moisture retention, flexibility, elasticity and the like are ensured, and the appearance is maintained in a fresh state with tension and gloss.

However, when affected by external factors such as ultraviolet rays, significant air drying, excessive skin washing, stress, smoking, etc., or when aging progresses, the degradation and alteration of collagen, which is the main component of the extracellular matrix, occurs. It occurs, the proliferation of fibroblasts slows down, or laminin 5 which is a main component of the basement membrane is decomposed or altered to destroy the basement membrane structure (see Non-Patent Document 2). As a result, the moisturizing function and elasticity of the skin are lowered, abnormal exfoliation of the keratin occurs, and changes accompanying aging of the skin, that is, wrinkles, dullness, disappearance of texture, loss of elasticity, and the like occur.
Therefore, it is considered that aging of the skin can be prevented or improved by promoting collagen production, fibroblast proliferation, or laminin 5 production.

Accordingly, it is desired to obtain a substance having a collagen production promoting action, a fibroblast proliferation action or a laminin 5 production promoting action from a highly safe natural product.
Until now, what has a collagen production promoting action includes, for example, those having a fibroblast proliferating action, such as a star fruit leaf extract (see Patent Document 1), a Kusunohagashi extract (see Patent Document 2), etc. Examples of those having a laminin 5 production promoting action, such as moon peach leaf extract (see Patent Document 3), oni strawberry extract (see Patent Document 4), and the like include, for example, cuckoo extract, licorice extract or flavonoid fraction thereof, Hyogi Extract, dicynox extract, lobster extract, koloha extract, whey extract (see patent document 5 above), onionis extract, merirot extract, palm extract, azuki bean extract (above patent document 6) Reference), soybean extract (see Patent Document 7) and the like have been reported.

However, a preparation derived from a natural product that has high safety and productivity, is inexpensive and can be used on a daily basis, and has an excellent type I collagen production promoting action, fibroblast proliferation action, or laminin 5 production promoting action. The current situation is not yet provided.
JP 2002-226323 A JP 2003-146837 A JP 2002-003390 A JP 2003-137801 A JP 2003-137767 A JP 2003-137768 A JP 2004-217618 A Marinkovich MP et al. , J. et al. Cell. Biol. 1992 199: 695-703 Lavker et al. , J. et al. Invest. Dermatol. 1979 73: 59-66

An object of the present invention is to solve the conventional problems and achieve the following objects.
That is, the present invention is a natural product that has high safety and productivity, is inexpensive and can be used on a daily basis, and has an excellent type I collagen production promoting effect, fibroblast proliferation effect, or laminin 5 production promoting effect. It aims at providing the anti-aging agent of origin.

  As a result of intensive studies by the present inventors to solve the above-mentioned problems, an extract of G. bicolor DC .: kinkisou is excellent in producing type I collagen. It was found useful as an anti-aging agent having a promoting action, a fibroblast proliferating action, and a laminin 5 production promoting action.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
<1> An anti-aging agent characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.
<2> The anti-aging agent according to the above <1>, wherein the extract of Kintokusa is an extract using a mixed solvent of water and ethanol.
<3> A type I collagen production promoter characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.
<4> A fibroblast proliferating agent characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.
<5> A laminin 5 production promoter characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.
<6> A skin external preparation containing the anti-aging agent according to any one of <1> to <2>.
<7> A cosmetic food or drink comprising the anti-aging agent according to any one of <1> to <2>.

According to the anti-aging agent of the present invention, skin aging, that is, wrinkles, dullness, disappearance of texture, elasticity, is achieved through at least one of the excellent type I collagen production promoting action, fibroblast proliferation action and laminin 5 production promoting action. It is possible to prevent and improve the deterioration of the property.
Moreover, since the anti-aging agent of this invention is excellent in safety | security and a usability | use_condition, it is suitable for mix | blending with a skin external preparation or cosmetics food / beverage products.

(Anti-aging agent)
The anti-aging agent of the present invention comprises G. bicolor DC .: Kintoso grass, or an extract thereof, and, if necessary, other components.
Kintokoshi is a perennial plant native to tropical Asia. When grown, the plant height is 30-60 cm, the stem is cylindrical, and the buds grow vigorously from the leaf buds and become fluffy. The leaves are stylized and alternate, with needle-shaped, serrated edges, and short hair, but are almost smooth, shiny, slightly thick, dark green on the front, and purple on the back. Kintokusa is easily available because it is wild in Amami-Oshima, southern Kyushu, Japan.

In the present invention, Kintoki can be extracted by a method generally used for plant extraction.
The extraction site is not particularly limited and can be appropriately selected depending on the purpose.For example, a leaf part, a stem part, a flower part, a seed, or an above-ground part or a root part containing these can be used. Suitable is the above-ground part (leaf part, stem part flower part, seed, or a mixed part thereof).
The gold raw grass as the raw material for extraction can be subjected to solvent extraction as it is or after pulverization with a crusher. Drying may be performed in the sun or using a commonly used dryer.
In addition, you may perform pre-processing, such as degreasing | defatting, with a nonpolar solvent, such as hexane and benzene. By performing pretreatment such as degreasing, the extraction treatment can be performed efficiently.

Examples of the solvent used for the extraction include water, a hydrophilic organic solvent, or a mixed solvent thereof, and these are preferably used at a temperature from room temperature to the boiling point of the solvent.
Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, propylene glycol, Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin.
In addition, when using the mixed solvent of the said hydrophilic organic solvent and water, in the case of a lower alcohol, 1-90 mass parts with respect to 10 mass parts of water; in the case of a lower aliphatic ketone, 10 masses of water In the case of polyhydric alcohol, it is preferable to add 1 to 90 parts by mass with respect to 10 parts by mass of water. In particular, a mixed solvent of ethanol and water is preferable.

In the present invention, it is not necessary to adopt a special extraction method when extracting a substance having an anti-aging action from Kintoki grass, and it can be extracted using any extraction device at room temperature or under reflux heating. .
Specifically, after putting the above-ground part of Kinki Grass into a treatment tank filled with the extraction solvent, and eluting soluble components by allowing to stand for 30 minutes to 2 hours with occasional stirring as necessary. A method of removing solids by filtration, evaporating the extraction solvent from the obtained extract, and drying. The amount of solvent used for extraction is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent. Moreover, when using the mixed solvent of water and ethanol as an extraction solvent, it is about 30 minutes-about 4 hours at 40-80 degreeC normally.

In addition, the extract of the kinoki grass includes both a dried product obtained by drying a diluted solution of the extracted solution, or a crudely purified product or a purified product thereof.
The resulting extract of Kintokusa can be used as it is as an anti-aging agent of the present invention without distilling off the solvent if the extraction solvent is highly safe. It is easier to use.
In addition, in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crudely purified product or a purified product thereof, treatments such as dilution, concentration, drying, and purification may be performed according to a conventional method. Good.

  In obtaining a dried extract, a carrier such as dextrin or cyclodextrin may be added to improve hygroscopicity. In addition, it is possible to carry out purification for the purpose of decolorization, deodorization and the like within a range that does not cause a decrease in the physiological activity of the extract of Kintoki grass. Specifically, purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

The anti-aging agent of the present invention is useful for preventing and improving skin wrinkles and reduction in elasticity through at least one of type I collagen production promoting action, fibroblast proliferation action, and laminin 5 production promoting action, High safety.
The anti-aging agent of the present invention has the above-described anti-aging action (type I collagen production promoting action, fibroblast proliferation action, and laminin 5 production promoting action), and is particularly excellent in usability and safety. These are suitable for blending into the following skin external preparations and cosmetic foods and beverages of the present invention.

(Skin external preparation)
The skin external preparation of this invention contains the anti-aging agent of this invention, and the other component suitably selected as needed.
Here, the form of the external preparation for skin is not particularly limited and can be appropriately selected from various uses. For example, ointment, cream, emulsion, lotion, pack, jelly, lip balm, lipstick, bath additive, astringent Etc.
In the present invention, the blending amount of the anti-aging agent with respect to the whole external preparation for the skin can be appropriately adjusted according to the purpose, but is 0.0001 to 10% by mass in terms of the extract of the kinki grass. Is preferable, and it is more preferable that it is 0.001-1 mass%.

The said external preparation for skin can add the other component suitably selected as needed in the range which does not impair the effect of this invention.
Examples of the other components include astringents, bactericides, antibacterial agents, ultraviolet absorbers, cell activators, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, and fragrances. Etc. These components may act synergistically when used in combination with the extract of Kinki-Kusa, resulting in superior effects than would normally be expected.
The external preparation for skin of the present invention is highly safe and effectively exhibits excellent type I collagen production promoting action, fibroblast proliferation action, and laminin 5 production promoting action, thereby reducing skin wrinkles and elasticity. It is useful for prevention and improvement, prevention of rough skin due to drying, and the like.

(Beauty food and drink)
The cosmetic food and drink of the present invention includes the anti-aging agent of the present invention and other components appropriately selected as necessary.
In the present invention, beauty foods and drinks are those that are less likely to harm human health and are orally administered in normal social life, such as foods, pharmaceuticals, quasi drugs, etc. It is not limited to the category, and means, for example, a wide range of foods including general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals.

  There is no restriction | limiting in particular in the form of the said beauty food-drinks, Although it can select suitably according to the objective, Beverages, such as a soft drink, a carbonated drink, a nutrition drink, a fruit drink, a lactic acid drink; Ice cream, ice Frozen desserts such as sorbet and shaved ice; noodles such as buckwheat, udon, harusame, gyoza skin, sushi mai, Chinese noodles and instant noodles; rice cakes, candy, gum, chocolate, tablet confectionery, snacks, biscuits, jelly, jam, cream , Baked confectionery, bread and other confectionery; crab, salmon, clam, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, sea bream, tocobushi, etc .; Processed fishery and livestock products such as ham and sausage; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, Oil and fat processed foods such as yotoning, whipped cream, dressing; seasonings such as sauce and sauce; curry, stew, parent and child rice bowl, rice bowl, miscellaneous cooking, Chinese rice bowl, bowl, tempura, eel rice, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs; various forms of health foods and nutritional supplements; pharmaceuticals such as tablets, capsules, drinks, troches, etc. External goods etc. are mentioned.

Examples of the other components include auxiliary raw materials or additives that are usually used in the production of cosmetic foods and drinks.
The raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose.For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid, Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, gum arabic Carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives and the like.

  In the present invention, the blending amount of the anti-aging agent of the present invention with respect to the entire cosmetic food and drink can be adjusted as appropriate according to the purpose, and may be within a range that does not impair the original taste of the cosmetic food or drink, It is preferably 0.001 to 50% by mass and more preferably 0.01 to 20% by mass in terms of the extract of the gold leaf. Moreover, in the case of the cosmetics food-drinks in the form of a granule, a tablet, or a capsule, it is preferable that it is 0.01-100 mass%, and it is more preferable that it is 5-100 mass%. In addition, about 1-1000 mg per day for an adult is appropriate for the intake of the extract of Kintokusa.

The cosmetic food and drink according to the present invention can be taken orally on a daily basis, and exerts at least one of its anti-aging effects very effectively by the action of an extract of Kintoki which is an active ingredient. Can do.
The anti-aging agent, the external preparation for skin, and the food and drink for cosmetics of the present invention are preferably applied to humans. Can also be applied.

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto.
(Production Example 1)
-Manufacture of water extract of Kintoki-
As a raw material for extraction, G. bicolor DC .: 200 g of pulverized ground material of Kintoki grass was put into 2000 mL of water and kept at 80 ° C. for 3 hours with gentle stirring. Filtered. The filtrate was concentrated under reduced pressure at 40 ° C. and further dried with a vacuum dryer to obtain an extract (powder).

(Production Example 2)
-Manufacture of 50% by weight ethanol extract of Kintoki-
As the raw material for extraction, G. bicolor DC .: 200 g of the ground portion of Kintoki grass was added to 2000 mL of 50 mass% ethanol (mass ratio of water to ethanol of 1: 1), The mixture was kept at 80 ° C. for 3 hours with gentle stirring and then filtered. The filtrate was concentrated under reduced pressure at 40 ° C. and further dried with a vacuum dryer to obtain an extract (powder).

(Production Example 3)
-Manufacture of gold extract from ethanol-
As a raw material for extraction, G. bicolor DC .: 200 g of pulverized ground material of Kintoki grass was added to 2000 mL of ethanol and kept at 80 ° C. for 3 hours with gentle stirring. Filtered. The filtrate was concentrated under reduced pressure at 40 ° C. and further dried with a vacuum dryer to obtain an extract (powder).

Table 1 shows the yield (mass%) of the extract of Kintoki grass obtained in Production Examples 1 to 3.
(Table 1)
Production example Extraction solvent Yield (% by mass)
――――――――――――――――――――――――――――――
1 Water 9.4
2 50% ethanol 6.9
3 Ethanol 5.3
――――――――――――――――――――――――――――――

(Example 1)
<Type I collagen production promoting action test>
Using each extract of Production Examples 1 to 3 as a test sample, the type I collagen production promoting effect was tested as follows.
Human normal fibroblasts (Detroit 551) were cultured in a medium (10% FBS, 1% NEAA, 1 mmol / L sodium pyruvate-containing MEM), and then collected by trypsin treatment. The collected cells were diluted with the above medium to a concentration of 2 × 10 ⁵ cells / mL, seeded in a 96-well microplate at 100 μL per well, and cultured overnight.
After completion of the culture, the medium was removed, and 100 μL of the test sample (100 ppm) dissolved in 0.5% FBS-containing MEM was added to each well and cultured for 3 days. Thereafter, the amount of collagen in the medium in each well was measured by ELISA.

The type I collagen production promotion rate was calculated with the amount of type I collagen production when no sample was added as 100%. The calculation method of the type I collagen production promotion rate is as follows.
Type I collagen production promotion rate (%) = A / B × 100
A: Type I collagen amount when test sample is added B: Type I collagen amount when no test sample is added

Table 2 shows the type I collagen production promotion rate (%) of each sample.
(Table 2)
Extraction solvent Type I collagen production promotion rate (%)
(Sample concentration 100ppm)
―――――――――――――――――――――――――――――――――――
Example 1-1 Water 211.3
Example 1-2 Water: Ethanol (1: 1) 225.6
Example 1-3 Ethanol 201.2
―――――――――――――――――――――――――――――――――――
From the results of Table 2, it was confirmed that the extract of Kintoki in Production Examples 1 to 3 has an action of promoting type I collagen production in human fibroblasts.
In particular, it was found that the extract of Kintoki grass extracted with a mixed solvent of water and ethanol has a high type I collagen production promoting effect (Example 1-2).

(Example 2)
<Fibroblast proliferation promoting test>
Using each extract of Production Examples 1 to 3 as a test sample, the fibroblast proliferation promoting action was tested as follows.
Human normal skin fibroblasts (NB1RGB) were cultured with 10% FBS-containing α-MEM and then collected by trypsin treatment. The collected cells were diluted with α-MEM containing 5% FBS to a concentration of 7.0 × 10 ⁴ cells / mL, seeded in a 96-well microplate at 100 μL per well, and cultured overnight.
After completion of the culture, 100 μL of a test sample (400 ppm) dissolved in α-MEM containing 5% FBS was added to each well and cultured for 3 days.
The skin fibroblast proliferation effect was measured using the MTT assay. That is, 100 μL of medium was removed from each well, and 20 μL of MTT dissolved in PBS (−) at a final concentration of 5 mg / mL was added to each well. After culturing for 4.5 hours, 100 μL of 0.01 mol / L hydrochloric acid solution in which 10% SDS was dissolved was added to each well. After overnight culture, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. In addition, a blank test was performed and corrected in the same manner.

The calculation method of the fibroblast proliferation promotion rate is as follows.
Fibroblast growth rate (%) = (St−Sb) / (Ct−Cb) × 100
St: Absorbance in cells with added test sample Sb: Absorbance in blank test with added test sample Ct: Absorbance in cells without added test sample Cb: Absorbance in blank test without added test sample

Table 3 shows the fibroblast proliferation promotion rate (%) of each sample.
(Table 3)
Extraction solvent Fibroblast growth promotion rate (%)
(Sample concentration 400 ppm)
―――――――――――――――――――――――――――――――――――
Example 2-1 Water 122.3
Example 2-2 Water: ethanol (1: 1) 127.7
Example 2-3 Ethanol 125.3
―――――――――――――――――――――――――――――――――――
From the results in Table 3, it was confirmed that the extract of Kintoki in Production Examples 1 to 3 had a human fibroblast proliferation action.
In particular, it was found that the extract of Kintoki grass extracted with a mixed solvent of water and ethanol has a high fibroblast proliferation action (Example 2-2).

(Example 3)
<Laminin 5 production promoting action test>
Using each extract of Production Examples 1 to 3 as a test sample, laminin 5 production promoting action was tested as follows.
Human keratinocytes are seeded in a 24-well plate, cultured at 37 ° C. under 5% CO 2 -95% air in each sample-added medium (sample concentration: 50 μg / mL) for 24 hours, and then 100 μL of the supernatant is added. After transferring to an ELISA plate and adsorbing to the plate at 4 ° C. overnight, the solution was discarded and washing was performed with a phosphate physiological buffer solution (PBS-T) containing 0.05 mass% Tween20. Thereafter, a blocking operation was performed with a phosphate physiological buffer containing 1% by mass bovine serum albumin. The solution was discarded, washed with a phosphate physiological buffer solution (PBS-T) containing 0.05% by mass Tween 20, and reacted with anti-human laminin 5 antibody (mouse IgG, manufactured by Chemicon). The solution was discarded, washed with a phosphate physiological buffer solution (PBS-T) containing 0.05% by mass Tween 20, and reacted with biotin-labeled antibody mouse IgG (Amersham Biosciences). The solution is discarded, washed with a phosphate physiological buffer solution (PBS-T) containing 0.05% by weight Tween 20, reacted with an avidin-biotinylated peroxidase complex, and then subjected to the same washing operation to develop a color reaction. Went.
The laminin 5 production promotion rate was calculated with the absorbance when no sample was added as 100%. Table 4 shows the laminin 5 production promotion rate (%) of each sample.

(Table 4)
Extraction solvent Laminin 5 production promotion rate (%)
(Sample concentration 12.5ppm)
――――――――――――――――――――――――――――――――――――
Example 3-1 Water 131.3
Example 3-2 Water: Ethanol (1: 1) 137.0
Example 3-3 Ethanol 129.1
――――――――――――――――――――――――――――――――――――
From the results in Table 4, it was confirmed that the extract of Kinki-Kusa in Production Examples 1 to 3 had a laminin-5 production promoting action of keratinocytes.
In particular, it was found that the extract of Kintoki grass extracted with a mixed solvent of water and ethanol has a high laminin 5 production promoting effect (Example 3-2).

Example 4
<Emulsion>
An emulsion having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Present Invention 0.01
Jojoba oil 4.0
Placenta extract 0.1
Olive oil 2.0
Squalane 2.0
Cetanol 2.0
Glyceryl monostearate 2.0
Polyoxyethylene cetyl ether (20E.O) 2.5
Oleic acid polyoxyethylene sorbitan (20E.O) 2.0
Stearyl glycyrrhetinate 0.1
1,3-butylene glycol 3.0
Hinokitiol 0.15
Fragrance 0.05
Purified water balance

(Example 5)
<Cream>
A cream having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Invention 0.05
Aloe extract 0.1
Liquid paraffin 5.0
Sara honey bees 4.0
Squalane 10.0
Cetanol 3.0
Lanolin 2.0
Stearic acid 1.0
Oleic acid polyoxyethylene sorbitan (20E.O) 1.5
Glyceryl monostearate 3.0
Oil-soluble licorice extract 0.1
1,3-butylene glycol 6.0
Methyl paraoxybenzoate 1.5
Fragrance 0.1
Purified water balance

(Example 6)
<Cream>
A cream having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Invention 0.05
Ages Extract 0.1
Polyethylene glycol monostearate 2.0
Self-emulsifying glyceryl monostearate 5.0
Stearic acid 2.0
Cetanol 2.0
Squalane 12.0
Macadamia nut oil 3.0
Methylpolysiloxane 0.2
Fragrance 0.01
Preservative (Methyl paraoxybenzoate) 0.15
Oil-soluble licorice extract 0.1
1,3-butylene glycol 7.0
Xanthan gum 0.2
Purified water balance

(Example 7)
<Serum>
A serum having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Present Invention 0.01
Chamomile extract 0.1
Xanthan gum 0.3
Hydroxyethyl cellulose 0.1
Carboxyvinyl polymer 0.1
1,3-butylene glycol 4.0
Dipotassium glycyrrhizinate 0.1
Glycerin 2.0
Potassium hydroxide 0.25
Fragrance 0.01
Preservative (Methyl paraoxybenzoate) 0.15
Ethanol 2.0
Purified water balance

(Example 8)
<Serum>
A serum having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Invention 0.05
Mulberry extract 0.1
Carboxyvinyl polymer 0.2
Xanthan gum 0.2
Sodium hyaluronate 0.1
Polyethylene glycol 3.0
Glycerin 6.0
1,3-butylene glycol 3.0
Dipotassium glycyrrhizinate 0.1
Preservative (Methyl paraoxybenzoate) 0.15
L-Arginine 0.15
Purified water balance

Example 9
<Pack>
A pack having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Invention 0.05
Yokuinin extract 0.1
Polyvinyl alcohol 15.0
Polyethylene glycol 3.0
Propylene glycol 7.0
Ethanol 10.0
Methyl paraoxybenzoate 0.05
Dipotassium glycyrrhizinate 0.1
Fragrance 0.05
Purified water balance

(Example 10)
<Cleansing lotion>
A cleaning lotion having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Invention 0.05
Lavender extract 0.1
1,3-butylene glycol 4.0
Glycerin 4.0
Dipropylene glycol 2.0
Polyoxyethylene polyoxypropylene 0.7
Decyl tetradecyl ether ethanol 3.0
Dipotassium glycyrrhizinate 0.1
Fragrance 0.001
Preservative (Methyl paraoxybenzoate) 0.15
Purified water balance

(Example 11)
<Emulsion>
An emulsion having the following composition was produced by a conventional method.
(mass%)
Extract of Kinki Grass of the Invention 0.05
Rosemary extract 0.1
Polyoxyethylene sorbitan monostearate 1.0
Tetraoleic acid polyoxyethylene sorbit 1.5
Lipophilic glyceryl monostearate 1.0
Stearic acid 0.5
Behenyl alcohol 1.5
Cetyl palmitate 0.5
Squalane 10.0
Methyl polysiloxane 0.5
Perfume appropriate amount 1,3-butylene glycol 7.0
Stearyl glycyrrhetinate 0.1
Xanthan gum 0.1
Purified water balance

(Example 12)
<Tablet dietary supplement>
The following mixture was tableted to produce a tablet-shaped dietary supplement.
50 g of Kintoki extract of the present invention
178g powdered sugar (sucrose)
Sorbit 10g
Glycerin fatty acid ester 12g

(Example 13)
<Granular dietary supplement>
The following mixture was formed into granules to produce a dietary supplement.
34 g of Kintoki extract of the present invention
1000g beet oligosaccharide
Vitamin C 167g
Glycerin fatty acid ester 12g
Stevia extract 10g

The anti-aging agent of the present invention has high safety and productivity, is inexpensive and can be routinely used, and has an excellent type I collagen production promoting effect, fibroblast proliferation effect, or laminin 5 production promoting effect. .
The topical skin preparation containing the anti-aging agent of the present invention is useful for preventing and improving skin wrinkles and reduction in elasticity, preventing rough skin, and preventing rough skin, such as ointments, creams, emulsions, lotions, packs, Widely used in jelly, lip balm, lipstick, bath salt, astringent, etc.
In addition, cosmetic foods and drinks to which the anti-aging agent of the present invention is added have excellent antioxidant and anti-aging effects even when taken orally, and are excellent in safety. Widely used in various beauty foods and drinks such as food.

Claims (7)

  An anti-aging agent characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.   The anti-aging agent according to claim 1, wherein the extract of Kintoki grass is an extract of a mixed solvent of water and ethanol.   A type I collagen production-promoting agent characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.   A fibroblast proliferating agent characterized by containing at least one of Kintoki grass and an extract thereof as an active ingredient.   A laminin-5 production promoter characterized by containing at least one of Kinki Grass and an extract thereof as an active ingredient.   The skin external preparation containing the anti-aging agent in any one of Claim 1 to 2.   Beauty food-drinks containing the anti-aging agent in any one of Claim 1 to 2.