JP4672304B2 - Lipolysis accelerator - Google Patents

Description

  The present invention relates to a lipolysis promoter that can contribute to the prevention and suppression of obesity by promoting the degradation of fat accumulated in fat cells responsible for the synthesis and accumulation of fat in vivo, and cosmetics and foods and drinks. About.

  When the intake energy is excessive with respect to the consumed energy, the excess fat is accumulated as neutral fat in white fat cells. Obesity caused by the accumulation of body fat is not only cosmetically unfavorable, but also causes various diseases such as arteriosclerosis and diabetes. Moreover, it is an age of satiety, and obesity due to overeating, lack of exercise, stress, etc. has increased, and this has become a big problem from the viewpoint of beauty regardless of gender. As described above, accumulation of body fat and the like is not preferable for health, and reduction of body fat and the like, and prevention of accumulation of body fat and the like have become important problems.

  In order to solve the above-mentioned problems, various kinds of herbal medicines having an action of promoting lipolysis have been conventionally reported (see Patent Document 1, Patent Document 2, and Patent Document 3). In addition, the applicant of the present application has already demonstrated that procyanidin extract (see Patent Document 4), Bergenin extract (see Patent Document 5), and Himalayan Yukinoshita extract (see Patent Document 6) have excellent blood lipid improvement. It discloses that it has an effect and an anti-obesity effect.

  However, it is possible to contribute to the prevention and control of obesity by promoting the degradation of fat accumulated in fat cells responsible for the synthesis and accumulation of fat in the living body, and it is an inexpensive and highly safe natural product system The demands of consumers for the lipolysis accelerators are extremely high, and the provision of further new lipolysis accelerators is strongly demanded.

JP 2000-63227 A JP 2002-275078 A JP-A-10-158181 JP-A-9-291039 JP-A-11-180869 Japanese Patent Laid-Open No. 11-209299

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention relates to a lipolysis accelerator that can contribute to the prevention and suppression of obesity by promoting the degradation of fat accumulated in fat cells responsible for the synthesis and accumulation of fat in vivo, and the lipolysis It aims at providing the food / beverage products containing the cosmetics containing this accelerator, and this lipolysis promoter.

  As a result of intensive studies by the inventor in order to solve the above-mentioned problems, at least one of an extract of birch family birch and an extract of grass family kumaza is obese through promotion of fat degradation in whole body or local adipose tissue. It was found that obesity can be effectively suppressed and prevented by improving the constitution and preventing an increase in adipose tissue.

This invention is based on the said knowledge by this inventor, and as a means for solving the said subject, it is as follows. That is,
<1> A lipolysis promoter characterized by containing at least one of an extract of birch family birch and an extract of Gramineae.
<2> A cosmetic comprising the lipolysis promoter according to <1>.
<3> A food or drink comprising the lipolysis promoter according to <1>.

  At least one of the birch and birch extract is effective in improving obesity through the promotion of fat degradation in systemic or local adipose tissue, and preventing obesity by increasing adipose tissue. There is nothing known to date about suppression and prevention, and these are new findings by the present inventors.

  According to the present invention, various problems in the prior art can be solved, and the systemic or local adipose tissue can be reduced through the action of promoting lipolysis to effectively suppress obesity or improve the obesity constitution. Further, the lipolysis promoter of the present invention is suitable for blending into a slimming cosmetic because it is excellent in use feeling and safety when applied to the skin. Moreover, the lipolysis promoter of the present invention has an excellent lipolysis promoting effect even when taken orally, is excellent in safety, and is suitably blended in various foods and drinks.

(Lipolysis accelerator)
The lipolysis promoter of the present invention contains at least one of an extract of birch family birch and an extract of Gramineae, and further contains other components as necessary.
In the present invention, the “extract” includes an extract obtained from an extraction raw material by an extraction process, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or these Either a crude product or a purified product is included.

The birch is a plant belonging to the family Vetulaceae , and its scientific name is Betula alba L. et al. It is. The extraction site of birch is not particularly limited and can be appropriately selected according to the purpose. For example, leaves, flowers, branches, bark, or a mixture thereof is preferable, and among these, bark is Particularly preferred.
The Kumazasa is a plant belonging to the Gramineae family and has a scientific name of Sasa veitii ERRD . There is no restriction | limiting in particular as an extraction site | part of this Kumazasa, According to the objective, it can select suitably, For example, a leaf, a stem, or these mixtures are suitable, A leaf is especially preferable among these.

  The birch or kumazasa as the raw material for extraction can be obtained by drying or pulverizing as it is using a crusher and subjecting it to solvent extraction. Drying may be performed in the sun or using a commonly used dryer. The birch or Kumazasa may be used as a raw material for extraction after pretreatment such as degreasing with a nonpolar solvent such as hexane or benzene. In addition, the extraction process by a polar solvent can be performed efficiently by performing pretreatments, such as degreasing.

  Although details of the lipolysis-promoting substance contained in at least one of the birch extract and the kumazasa extract are unknown, the lipolysis-promoting substance can be easily made by an extraction method generally used for plant extraction. Can be extracted.

As the solvent used for the extraction, it is preferable to use water, a hydrophilic organic solvent, or a mixture thereof at room temperature or a temperature not higher than the boiling point of the solvent.
Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, propylene glycol, Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin, and a mixed solvent of the hydrophilic organic solvent and water can be used.
In addition, when using the mixed solvent of the said water and a hydrophilic organic solvent, in the case of a lower alcohol, it is 1-90 mass parts with respect to 10 mass parts of water, and in the case of a lower aliphatic ketone, it is 10 mass parts of water. It is preferable to add 1-40 mass parts with respect to it. In the case of a polyhydric alcohol, it is preferable to add 1 to 90 parts by mass with respect to 10 parts by mass of water.

  In the present invention, it is not necessary to employ a special extraction method for extracting a lipolysis-promoting substance from birch or kumazasa as an extraction raw material, and the extraction can be performed using an arbitrary apparatus at room temperature or under reflux heating.

  Specifically, after adding birch or Kumazasa as an extraction raw material to a treatment tank filled with an extraction solvent, and further stirring as necessary, the mixture was allowed to stand for 30 minutes to 2 hours to elute soluble components, The solid matter is removed by filtration, and the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of the extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent. Moreover, when using the mixed solvent of water and ethanol as an extraction solvent, it is about 30 minutes-about 4 hours at 40-80 degreeC normally. It should be noted that the extract obtained by extraction with a solvent can be used as an active ingredient of the lipolysis promoter of the present invention as long as the extraction solvent is highly safe. The dried product is easier to use.

  The obtained birch extract or Kumazasa extract is diluted and concentrated according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude or purified product thereof. Treatments such as drying and purification may be applied.

  The obtained birch extract or Kumazasa extract can be used as it is as a lipolysis promoter, but a concentrated solution or a dried product thereof is easier to use. In obtaining a dried extract, a carrier such as dextrin or cyclodextrin may be added to improve hygroscopicity. Further, since the birch extract or Kumazasa has a peculiar odor, it is possible to perform purification for the purpose of decolorization, deodorization, etc. within a range not causing a decrease in its physiological activity. When added to foods and drinks, it is not used in large quantities, so there is no practical problem even if it is not purified. Specifically, purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

  The lipolysis promoter of the present invention has an extremely high lipolysis-promoting action, contributes to the suppression and prevention of obesity and the improvement of obesity constitution through the promotion of fat degradation in systemic or local adipose tissue, and high Since it has been confirmed that it has safety and is not digested in the digestive tract, it can be suitably used for the following cosmetics and foods and drinks of the present invention.

(Cosmetics)
The cosmetic of the present invention contains the lipolysis promoter of the present invention, and further contains other components appropriately selected as necessary.

  Here, the cosmetic is not particularly limited and may be appropriately selected depending on the purpose. Examples thereof include ointments, creams, emulsions, lotions, packs, jellies, lip balms, lipsticks, bathing agents, and the like. It is done.

  The blending amount of the lipolysis promoter with respect to the entire cosmetic can be adjusted as appropriate depending on the type of cosmetic, physiological activity of the extract, etc., but is 0.005 in terms of the birch extract or Kumazasa extract. -10 mass% is preferable.

  The cosmetic compounded with the lipolysis accelerator can use various main ingredients and auxiliaries usually used for the production of cosmetics and other components as long as the effects of the present invention are not impaired. . The cosmetics of the present invention are not limited to those in which only the lipolysis promoter of the present invention is the main agent for the suppression and prevention of obesity and the improvement of obesity constitution.

-Other ingredients-
The other components are not particularly limited as long as they do not interfere with the lipolytic action, and include components appropriately selected according to the purpose. Examples include whitening agents, astringents, bactericidal / antibacterial agents, ultraviolet absorbers, Moisturizers, cell activators, anti-inflammatory / antiallergic agents, antioxidant / active oxygen scavengers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, etc. . These components, when used in combination with at least one of the birch extract and the kumazasa extract, may act synergistically to provide a superior use effect than normally expected.

(Food)
The food and drink of the present invention contains the lipolysis promoter of the present invention, and further contains other components appropriately selected as necessary.
Here, the food and drink are those which are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to the category of products, etc., and means, for example, a wide range of foods that are taken orally, including general foods, health foods, health functional foods, quasi drugs, pharmaceuticals, and the like.

  There is no restriction | limiting in particular as said food-drinks, Although it can select suitably according to the objective, For example, drinks, such as a soft drink, a carbonated drink, a nutritive drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, shaved ice Noodles such as buckwheat noodles, udon, harusame, gyoza skin, Chinese noodles, instant noodles, etc .; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery Confectionery such as bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallops, abalone, sea urchin, crabs, tocobushi, etc .; Fishery and livestock processed foods such as sausages; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, show Oils and processed oils such as ning, whipped cream and dressing; seasonings such as sauces and sauces; curry, stew, oyakodon, rice bowl, miscellaneous foods, Chinese rice bowl, and rice cake, tempura, eel rice cake, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburg, meatballs; various forms of health and nutritional supplements; pharmaceuticals such as tablets, capsules, drinks, troches, etc. Goods. In addition, the said food / beverage is not limited to the said illustration.

As said other component, the auxiliary | assistant raw material or additive normally used in manufacturing the said food / beverage is mentioned.
The raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose.For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid, Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, gum arabic , Carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinamide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives, and the like.

  The amount of the lipolysis accelerator of the present invention in the food and drink varies depending on the type of the food and drink to be targeted and cannot be specified unconditionally, but it is added within a range that does not impair the original taste of the food or drink. 0.001-50 mass% is preferable normally with respect to various object food-drinks, and 0.01-20 mass% is more preferable. Moreover, in the case of the food / beverage of a granule, a tablet, or a capsule form, 0.01-100 mass% is preferable normally, and 5-100 mass% is more preferable. In addition, it is appropriate that the intake of at least one of birch extract and Kumazasa extract is about 1-1000 mg per day for an adult.

  The food and drink of the present invention can be taken orally on a daily basis, and achieves an action of promoting lipolysis extremely effectively by the action of at least one of the birch extract and the kumazasa extract, which are active ingredients. Can do.

  The lipolysis promoter, cosmetics, and food and drink of the present invention are preferably applied to humans, but are applicable to animals other than humans as long as their respective effects are exhibited. You can also

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Production Example 1)
-Production of birch extract-
To 100 g of dried birch bark, add 1000 mL of 50% by mass ethanol (mass ratio of water to ethanol of 1: 1), heat extract with a reflux extractor at 80 ° C. for 2 hours, and filter while hot did. The same extraction process was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and dried to obtain 4 g of birch extract.

(Production Example 2)
-Production of Kumazasa extract-
Add 1000 mL of 50 mass% ethanol (mass ratio of water to ethanol of 1: 1) to 100 g of chopped dried kumazasa leaves, heat extract with a reflux extractor at 80 ° C. for 2 hours, and filter while hot did. The same extraction process was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and dried to obtain 5 g of Kumazasa extract.

Example 1
-Lipolysis accelerating action test-
About the birch extract of the manufacture example 1 and the kumazasa extract of the manufacture example 2, the lipolysis acceleration | stimulation effect | action was tested by the method of the following rod bell.
Rodbell method [Rodbell M. , J .; Biol. Chem. , 239, 375 (1964)], free adipocytes were prepared from a testicular adipose tissue of Wistar male rats (body weight 150 to 200 g) using a collagenase solution. 10 μL of Hank's buffer containing bovine serum albumin prepared so that the final concentration of birch extract of Production Example 1 or Kumazasa Extract of Production Example 2 is 400 μg / mL and 100 μg / mL in 90 μL of a suspension of free adipocytes And reacted at 37 ° C. for 90 minutes. After the reaction, the amount of fatty acid released in 5 μL of the obtained reaction solution was measured using NEFA C-Test Wako (manufactured by Wako Pure Chemical Industries, Ltd.). The lipolysis acceleration rate was calculated from the obtained measured value according to the following formula 1. The results are shown in Table 1.
<Formula 1>
Lipolysis promotion rate (%) = (A / B) × 100
However, in the said Numerical formula 1, A represents the amount of free fatty acids at the time of test sample addition. B represents the amount of free fatty acid when no test sample was added (control).

表１の結果から、シラカバ抽出物及びクマザサ抽出物は脂肪細胞中の脂肪の分解を促進することが確認され、特にシラカバ抽出物が高い脂肪分解促進効果を有することが認められた。

From the results in Table 1, it was confirmed that the birch extract and the kumazasa extract promoted the degradation of fat in adipocytes, and in particular, the birch extract was found to have a high lipolysis promoting effect.

( Reference Example 2)
A lotion containing the birch extract of Production Example 1 having the following composition (hereinafter referred to as “the lotion of the present invention”) was prepared according to a conventional method.
<Composition of the lotion of the present invention>
1.0 g of birch extract from Production Example 1
Ethanol 5.0g
Propylene glycol 5.0g
Hydrophilic surfactant (polyoxyethylene cetyl ether) 0.5g
Sodium hyaluronate 0.05g
Perfume appropriate amount Preservative (hinokitiol, ethyl paraoxybenzoate) 0.1g
Purified water balance
Total 100g

  Moreover, about the lotion of this invention, except not including the birch extract of manufacture example 1, the comparative lotion of the same composition as the lotion of this invention was prepared by the conventional method.

The following evaluation tests were performed on the obtained lotion of the present invention and comparative lotion as follows.
BMI [Body Mass index = body weight (kg) / {height (m) × height (m)}] indicating 30 degrees of obesity is 30 ± 3, and the abdomen after bathing every day for 3 weeks (Front, side), after applying to the inside of the thigh and massaging, (1) effect of increasing / decreasing the thickness of subcutaneous fat, (2) effect of increasing / decreasing skin flexibility, (3) increase / decrease of skin fineness For the effect and (4) the degree to which the sweat is greasy, sensory evaluation was performed in 1 to 5 stages (indicating that the larger the number, the more effective). The results are shown in Table 2.

表２の結果から、本発明の化粧水は、比較化粧水に比べて、（１）皮下脂肪の厚みの増加効果、（２）皮膚の柔軟性の増加効果、（３）肌のきめ細かさを向上する効果、及び（４）汗が脂ぎっているのを防止する効果があることが認められる。

From the results in Table 2, the lotion of the present invention has (1) an effect of increasing the thickness of subcutaneous fat, (2) an effect of increasing the flexibility of the skin, and (3) fineness of the skin as compared with the comparative lotion. It is recognized that there is an effect of improving and (4) an effect of preventing sweat from becoming greasy.

(Formulation Reference Example 1) -Emulsion-
An emulsion having the following composition was produced by a conventional method.
1 g of birch extract from Production Example 1
Jojoba oil 4g
2g olive oil
Squalane 2g
Cetanol 2g
2g glyceryl monostearate
2.5 g of polyoxyethylene cetyl ether (20E.O)
Oleic acid polyoxyethylene sorbitan (20E.O) 2g
Twilight extract 0.1g
Ginkgo biloba extract 0.1g
Conchiolin 0.1g
Oat extract 0.1g
Chamomile extract 0.1g
1,3-butylene glycol 3g
Methyl paraoxybenzoate 0.15g
Fragrance 0.05g
Purified water balance
Total 100g

(Formulation Reference Example 2)-Lotion-
A lotion having the following composition was produced by a conventional method.
2 g of birch extract from Production Example 1
Glycerin 3g
1,3-butylene glycol 3g
Oleic acid polyoxyethylene sorbitan (20E.O) 0.5g
Methyl paraoxybenzoate 0.15g
Citric acid 0.1g
Sodium citrate 0.1g
Oil soluble licorice extract 0.1g
Seaweed extract 0.1g
Xylobiose Mixture 0.5g
Kujin extract 0.1g
Fragrance 0.05g
Purified water balance
Total 100g

(Formulation Example 3) -Cream-
A cream having the following composition was produced by a conventional method.
1 g of Kumazasa extract from Production Example 2
Liquid paraffin 5g
Salami beeswax 4g
Setanol 3g
Squalane 10g
Lanolin 2g
Stearic acid 1g
Oleic acid polyoxyethylene sorbitan (20E.O) 1.5g
3g glyceryl monostearate
1,3-butylene glycol 6g
Yeast extract 0.1g
Perilla extract 0.1g
Linden extract 0.1g
Jiuyu Extract 0.1g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water balance
Total 100g

(Formulation Reference Example 4) -Pack-
A pack having the following composition was produced by a conventional method.
5 g of birch extract from Production Example 1
Polyvinyl alcohol 15g
Polyethylene glycol 3g
7g of propylene glycol
Ethanol 10g
Sage extract 0.1g
Toki extract 0.1g
Carrot extract 0.1g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water balance
Total 100g

(Formulation Example 5) -Pack-
A pack having the following composition was produced by a conventional method.
3 g of birch extract from Production Example 1
3 g of Kumazasa extract from Production Example 2
Polyvinyl alcohol 15g
Polyethylene glycol 3g
7g of propylene glycol
Ethanol 10g
Sage extract 0.1g
Toki extract 0.1g
Carrot extract 0.1g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water balance
Total 100g

(Formulation Example 6) -Tablet nutritional supplement-
The following mixture was tableted to produce a tablet-shaped dietary supplement.
Kumazasa extract of production example 2 ... 50g
Powdered sugar (sucrose) 178g
Sorbit ・ ・ ・ 10g
Glycerin fatty acid ester 12g

(Formulation Example 7) -Tablet nutritional supplement-
The following mixture was tableted to produce a tablet-shaped dietary supplement.
Birch extract of Production Example 1 30g
Kumazasa extract of Production Example 2 20g
Powdered sugar (sucrose) 178g
Sorbit ・ ・ ・ 10g
Glycerin fatty acid ester 12g

(Formulation Reference Example 8)-Granular dietary supplement-
The following mixture was formed into granules to produce a granular dietary supplement.
Birch extract of Production Example 1 34g
Beet oligosaccharide ... 1000g
Vitamin C ... 167g
Stevia extract ... 10g

The lipolysis promoter of the present invention is capable of improving obesity and preventing increase of adipose tissue by promoting the degradation of fat accumulated in fat cells responsible for fat synthesis and accumulation in vivo. Can be used for cosmetics and foods.
Since the cosmetic of the present invention has an excellent lipolysis promoting effect and is excellent in safety, it is widely used, for example, in ointments, creams, emulsions, lotions, packs, jellies, bathing agents and the like.
The food and drink of the present invention has an excellent lipolysis-promoting effect even when taken orally, and is also excellent in safety. Therefore, it is widely used in various foods and drinks such as health and nutritional supplements.

Claims (3)

A lipolysis promoter characterized by containing an extract of Gramineae Kumazasa, which is extracted by heating at 40 to 80 ° C. for 30 minutes to 4 hours with 50% by weight ethanol (mass ratio of water to ethanol of 1: 1). . The lipolysis promoter according to claim 1, wherein 50% by mass of ethanol is added in a mass ratio of 5 to 15 times with respect to Gramineae, and heat extraction is performed under reflux heating. The extract of Gramineae Kumazasa is an extract obtained by adding 50 mass% ethanol in a mass ratio with respect to the leaves of the Gramineae Kumazasa and heating and extracting for 2 hours under reflux heating at 80 ° C. The lipolysis promoter according to any one of 2 above.