JP2007230881A - Anti-fatigue composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an anti-fatigue composition that further reduces a mental fatigue by including both a rice-derived γ-aminobutyric acid and another rice-derived component, is derived from a natural edible vegetable, has no adverse effect and is taken for a long period of time. <P>SOLUTION: The anti-fatigue composition comprises a rice extract extracted from rice embryo, etc., as an active ingredient. The rice extract contains a rice-derived γ-aminobutyric acid. The content of the rice-derived γ-aminobutyric acid is preferably 1-99 mass% calculated as γ-aminobutyric acid when the amount of the rice extract is 100 mass%. The rice extract preferably contains a rice-derived peptide. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an anti-fatigue composition comprising a naturally-derived component as an active ingredient.

γ-Aminobutyric acid is a type of amino acid widely distributed in the animal and plant kingdoms. It is present in the brain folds of animals and is the main inhibitory transmitter of nerves. It activates blood flow in the brain and supplies oxygen to the brain. It is known to increase the amount and promote the metabolic function of brain cells. In addition, it has been recognized to have an effect of improving symptoms such as headache, tinnitus, memory impairment, decreased motivation due to stroke sequelae, cerebral arteriosclerosis, etc., and an effect of lowering blood pressure by acting on the vasomotor center of extension.
Γ-Aminobutyric acid preparations (synthetic products) sold as pharmaceuticals have a brain metabolism promoting effect, and have been found to improve various symptoms of cerebrovascular disorders such as cerebral infarction and cerebral hemorrhage sequelae, and to suppress blood pressure increase. .
Then, what contains the foodstuff material which enriched (gamma) -aminobutyric acid is known as what has effects, such as a blood-pressure rise inhibitory effect, as a physiologically active function. In addition, the following patent document 1 and the patent document 2 are known as a technique relevant to the foodstuff material which enriched (gamma) -aminobutyric acid.

JP-A-9-107920 JP-A-11-155102

Under such a background, the present inventor has found that rice extract containing γ-aminobutyric acid derived from rice has an action of reducing mental fatigue and physical fatigue, particularly mental fatigue. Completed the invention. Moreover, it discovered that mental fatigue was further reduced by containing both (gamma) -aminobutyric acids and other rice-derived components (peptides, lipids, saccharides, etc.), and completed the present invention. Furthermore, the rice extract is a composition derived from a natural edible plant and has no side effects and can be ingested over a long period of time.
Accordingly, an object of the present invention is to provide a safe food composition, pharmaceutical composition or cosmetic composition having a function of reducing fatigue, particularly mental fatigue.

In order to solve the above problems, the anti-fatigue composition of the present invention is a rice extract extracted from at least one of rice germ, rice bran containing germ, germ rice, brown rice, germinated brown rice, and white rice (A ) As an active ingredient, and the rice extract (A) contains γ-aminobutyric acid (a-1) derived from rice.
The content of the rice-derived γ-aminobutyric acid (a-1) is preferably 1 to 99% by mass in terms of γ-aminobutyric acid when the rice extract is 100% by mass.
Furthermore, the rice extract (A) preferably contains rice-derived peptides (a-2).
The peptide content (a-2) is preferably 0.1 to 35% by mass in terms of protein when the content of the rice extract is 100% by mass.
Furthermore, it is preferable that the said rice extract (A) contains the saccharide | sugar (a-3) derived from rice.
Moreover, it is preferable that the said rice extract (A) contains the lipid (a-4) derived from rice.
Furthermore, it is preferable that the said rice extract (A) contains the dietary fiber (a-5) derived from rice.
The anti-fatigue composition preferably further contains γ-aminobutyric acid (B).

Hereinafter, the present invention will be described in detail.
The anti-fatigue composition of the present invention comprises, as an active ingredient, rice extract (A) extracted from at least one of rice germ, rice bran containing germ, and germ rice.
The rice extract (A) contains γ-aminobutyric acid (a-1) derived from rice.
The above-mentioned “rice-derived γ-aminobutyric acid (a-1)” means at least one of rice germ, rice bran containing germ, germ rice, brown rice, germinated brown rice, and white rice (hereinafter simply referred to as “rice germ” Γ-aminobutyric acid (hereinafter sometimes referred to as GABA) and its derivatives, which are produced by changing components (eg, glutamic acid, etc.) extracted from rice germ and the like. Say. Here, the derivatives of GABA in the present invention include salts, esters and the like, and more specifically, nicotinoyl γ-aminobutyric acid, γ-aminobutyric acid calcium salt, γ-aminobutyric acid sodium salt, γ-amino Examples include magnesium butyrate, GABA methyl ester, ethyl ester, propyl ester, isopropyl ester, butyl ester, and tert-butyl ester. In addition, these may contain only 1 type and may contain 2 or more types.

  In addition, the content of the γ-aminobutyric acid (a-1) is not particularly limited, but when the rice extract (A) is 100% by mass, in terms of γ-aminobutyric acid (a conversion factor of 7.36). 1 to 99% by mass, preferably 3 to 55% by mass, more preferably 4 to 30% by mass, and still more preferably 4 to 10% by mass. This is because if it is less than 1% by mass or exceeds 99% by mass, the function of reducing fatigue is weakened. In addition, content of the said (gamma) -aminobutyric acid (a-1) can be measured by the amino acid automatic analysis method of Japan Food Research Center, or HPLC, At least among these two measuring methods One should just be in the said range of content. Moreover, when measuring by HPLC, the measurement conditions of (gamma) -aminobutyric acid should just use the general method for measuring (gamma) -aminobutyric acid, and when measuring at least one of these measurement conditions, It only has to be within the above range. The nitrogen-γ-aminobutyric acid coefficient used at this time is 7.36.

  The above “rice-derived peptides (a-2)” is an amino acid extracted from rice germ or the like, or produced by changing components contained in rice germ or the like, and derivatives thereof, and amino acids, and The derivative is a peptide-bonded one and excludes the γ-aminobutyric acid (a-1).

  The content of the rice-derived peptides (a-2) is not particularly limited, but when the rice extract (A) is 100% by mass, 0.01 to 35% by mass, preferably in terms of protein, It can be 0.1-20 mass%, More preferably, it is 1-15 mass%, More preferably, it can be 3-10 mass%. At this time, the content Z (mass%) of the rice-derived peptides (a-2) is quantified by the Kjeldahl method when the rice extract (A) is 100 mass%, When the content of nitrogen is X and the content of the γ-aminobutyric acid (a-1) is Y, it can be obtained by the following formula.

Z = [X− (Y / 7.36)] × 6.25
Formula (1)

Note that 7.36 in the above formula (1) is a numerical value obtained by dividing the molecular weight of γ-aminobutyric acid (103.12) by the atomic weight of nitrogen (14.0067) (ie, nitrogen-GABA conversion factor). .25 is a nitrogen-protein conversion factor.
That is, the content (Z) of the peptide (a-2) derived from rice was converted from the content X of the total nitrogen component to the content Y of the γ-aminobutyric acid (a-1) in terms of nitrogen. It can be calculated by subtracting one and then multiplying by 6.25 which is a nitrogen-protein conversion factor.

  Moreover, the said rice extract (A) may contain the saccharide | sugar (a-3) derived from rice. The “rice-derived saccharide (a-3)” is not particularly limited as long as it is extracted from rice germ or the like. For example, amylose, amylopectin, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose , Maltose, maltitol, trehalose, alkylated trehalose, and the like.

  Although content of the said rice-derived saccharide | sugar (a-3) is not specifically limited, When content of the said rice extract (A) is 100 mass%, it is 10-70 mass%, Preferably it is 13-60 mass%. More preferably, the content is 15 to 58% by mass.

  Further, the rice extract (A) may contain rice-derived lipid (a-4). The “rice-derived lipid (a-4)” is not particularly limited as long as it is extracted from rice germ or the like, or produced by changing components contained in rice germ or the like. Examples thereof include, but are not limited to, monoglyceride, diglyceride, triglyceride, γ-oryzanol, tocopherol, tocotrienol, octasanol, sterol, terpenoid, sphingoglycolipid, and sphingophospholipid.

  Although content of the said rice origin lipid (a-4) is not specifically limited, When content of the said rice extract (A) is 100 mass%, 0.01-1 mass%, Preferably it is 0.1. -0.8 mass%, More preferably, it can be 0.2-0.7 mass%.

Moreover, the said rice extract (A) may contain the dietary fiber (a-5) derived from rice. The “rice-derived dietary fiber (a-5)” is not particularly limited as long as it is extracted from rice germ or the like, or is produced by changing the components contained in rice germ or the like. Insoluble dietary fiber or water-soluble dietary fiber is included, and examples thereof include, but are not limited to, cellulose, hemicellulose, chitosan, galactomannan, pectin, psyllium, carrageenan, and derivatives thereof.
The content of the rice-derived dietary fiber (a-5) is not particularly limited. When the content of the rice extract (A) is 100% by mass, the content is 0.1 to 5% by mass, preferably 0.8. It can be 2-4 mass%.

  Although the manufacturing method of the said rice extract (A) is not specifically limited, For example, rice germ etc. are immersed in water on the conditions of pH 2.5-7.5 and 50 degrees C or less, Then, it extracts with an acid, extraction It can be produced by purifying the product by ion exchange chromatography.

The rice used for the production of the rice extract (A) is not limited to varieties, but those having a high embryo weight ratio, for example, giant embryo rice and varieties having a large amount of γ-aminobutyric acid (eg, rice from North Sea No. 269, etc.) Is more preferable. The germinated rice can be easily prepared by polishing the rice with an ordinary rice mill (for example, RMA-150 manufactured by Yanagisawa Seisakusho) and removing the koji. The embryo can be separated from the endosperm portion-derived flour and koji using a suitable sieve (about 32 mesh) after the germinated rice is further ground by a grinding-type rice mill (eg, manufactured by Satake Seisakusho, TM5).
Further, it is preferable to use a degreased rice germ. This is because free fatty acids that cause deterioration of γ-aminobutyric acid can be removed.
The degreasing method is not particularly limited, but it can be carried out by extracting and separating oil with an oil-soluble solvent (degreasing solvent). Further, the degreasing solvent used at this time is not particularly limited as long as it is oil-soluble, and examples thereof include acetone and n-hexane, and n-hexane is particularly preferable. This is because free fatty acids can be efficiently removed, the extracted oil can be used as an edible oil, and the rice extract can be easily used as a food material.
Moreover, degreasing can also be performed by supercritical extraction. At this time, it is most preferable to use carbon dioxide gas.

The rice extract (A) can be produced by adding water prepared at a predetermined pH to rice germ or the like. By this treatment, glutamic acid decarboxylase present in the germ can act to convert glutamic acid into γ-aminobutyric acid. At this time, the proteolytic enzyme also acts simultaneously, the protein contained in rice germ and the like is decomposed, glutamic acid is supplied, and it can be efficiently converted to γ-aminobutyric acid.
At this time, the pH of water is preferably pH 2.5 to 7.5, preferably pH 3.0 to 7.0, and more preferably pH 5.5 to 6.0. The acid used for pH adjustment may be either an organic acid or an inorganic acid, preferably an organic acid such as acetic acid, citric acid or malic acid, or an inorganic acid such as hydrochloric acid, sulfuric acid or phosphoric acid. As the alkali, sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium carbonate, sodium phosphate or the like is used. Furthermore, the amount of water added at this time is not particularly limited, but it is preferable to add 2 to 10 times the amount of water such as rice germ. The temperature of the water and shaking conditions at this time are 50 ° C. or lower, usually 10 to 50 ° C., 50 to 150 strokes / minute for 20 minutes or longer, usually 20 minutes to 24 hours, preferably 80 to 120. It is preferable to shake at a stroke / minute for 40 minutes to 10 hours, more preferably at 40 ° C. at 100 strokes / minute for 4 to 8 hours.

  Moreover, the said rice extract (A) can be obtained by adding and extracting an acid to the immersion material obtained by immersion treatment as mentioned above. Specifically, an inorganic acid such as hydrochloric acid, sulfuric acid or phosphoric acid or an organic acid such as acetic acid, citric acid or malic acid is added to adjust to acidic conditions, preferably strongly acidic conditions of pH 1-2, and then 0 to 70 ° C. The mixture is shaken at 10 to 30 ° C., 50 to 150 strokes / minute for 5 minutes to 2 hours, and preferably 100 strokes / minute for 1 hour to extract γ-aminobutyric acid. Next, the extract is subjected to solid-liquid separation operations such as centrifugation (3000 rpm, 10 minutes) and filtration, and the supernatant (extract) is recovered.

Subsequently, this extract is purified and concentrated by ion exchange chromatography or the like.
The obtained γ-aminobutyric acid has a sweet umami taste and can be used as a food additive or seasoning. The amount used in that case may be appropriately determined according to the purpose.

  The anti-fatigue composition of the present invention may further contain γ-aminobutyric acid (B). Here, the “γ-aminobutyric acid (B)” is γ-aminobutyric acid obtained by a method different from the rice-derived γ-aminobutyric acid (a-1). And the one obtained by fermenting the rice extract (A), rice germ, etc., containing wheat, kimchi and the like as a medium.

The anti-fatigue composition of the present invention can be used as a material for various foods and drinks. Examples of food and drink include edible oil (salad oil), confectionery (gum, candy, caramel, chocolate, cookie, snack, jelly, gummy, tablet confection etc.), noodles (soba, udon, ramen etc.), dairy products ( Milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) and health foods (tablets , Capsules, etc.) and nutritional supplements (nutrient drinks, etc.). The anti-fatigue composition of the present invention may be appropriately blended with these foods and drinks.
These foods and drinks can be blended with various components depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. In addition, the anti-fatigue composition having a health maintenance function includes other antioxidants and health food ingredients such as antioxidants (reduced ascorbic acid (vitamin C), vitamin E, reduced form). Glutatin, tocotrienol, vitamin A derivative, lycopene, lutein, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sesamin, lignans, catechin, isoflavone, chalcone, tannins, flavonoids, Coumarin, isocoumarins, blueberry extract), health food ingredients (V. (vitamin) A, V.B1, V.B2, V.B6, V.B12, VC, VD, VE, VP, choline, niacin, pantothenic acid , Calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soy lecithin, taurine, donariella Rotein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium, heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kokushi, keihi, hawthorn , Ginger, ganoderma, swordfish extract, suppon, licorice, kokushi, keihi, hawthorn, ginger, ganoderma, plantain, chamomile, chamomile, dandelion, hibiscus, honey, boren, royal jelly, lime, lavender, rosehip, rosemary, Sage, bifidobacteria, faecalis, lacris, wheat germ oil, sesame oil, perilla oil, soybean oil, medium chain fatty acid, agaricus, ginkgo biloba extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA, DP A, green tea, cordyceps, garlic, bee, papaya, puer, propolis, meguri tree, yabushitake, royal jelly, saw palmetto, hyaluronic acid, collagen, gabba, harpseal oil, shark cartilage, glucosamine, lecithin, phosphatidylserine, Tanachi Carrot, Mulberry Leaf, Soybean Extract, Echinacea, Ezoukogi, Barley Extract, Olive Leaf, Olive Seed, Gymnema, Banaba, Salacia, Garcinia, Chitosan, St. John's Wort, Jujube, Carrot, Passion Flower, Broccoli, Placenta, Barley , Grape seed, peanut seed coat, bilberry, black cohosh, maria thistle, laurel, sage, rosemary, raffma, black vinegar, bitter gourd, maca, safflower, flax, oolong tea, flower spine, caffeine, capsaicin, ki Looligosaccharide, Glucosamine, Buckwheat, Citrus, Dietary fiber, Protein, Prune, Spirulina, Barley young leaf, Nucleic acid, Yeast, Shiitake, Plum meat, Amino acid, Deep-sea cocoon extract, Noni, Oyster meat, Suppon, Champignon, Psyllium, Acerola, Pineapple, Banana, Peach, Apricot, Melon, Strawberry, Raspberry, Orange, Fucoidan, Meshimakobu, Cranberry, Chondroitin sulfate, Zinc, Iron, Ceramide, Silk peptide, Glycine, Niacin, Chesttree, L-cysteine, Red wine leaf, Millet, Horsetail, Biotin, Centella Asiatica, Lotus Cup, Pycnogenol, Japanese cypress, Rhubarb, Clove, Rosemary, Catechin, Pual, Citric Acid, Beer Yeast, Merirot, Black Zinger, Ginger, Gajutsu, Nattokina Zeo, Benikouji, Tocotrienol, Lactoferrin, Cinnamon, Persimmon Buckwheat, Cocoa, Yuzu Seed Extract, Perilla Seed Extract, Lychee Seed Extract, Evening Primrose Extract, Black Rice Extract, α-Lipoic Acid, Raw Coffee Bean Extract) it can.

As a specific production method, the anti-fatigue composition is spray-dried or freeze-dried together with powdered cellulose, and this is easily incorporated into foods and drinks (instant foods, etc.) by making it into powders, granules, tablets or solutions. Can do. Further, the anti-fatigue composition can be dissolved in, for example, fats and oils, ethanol, glycerin, or a mixture thereof to form a liquid and added to a beverage or added to a solid food. If necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granules and added to a beverage or a solid food.
When the anti-fatigue composition of the present invention is applied to foods and drinks, the main purpose is to maintain beauty and health, so the total content of active ingredients is 1 to 20 wt. % Is preferred.

  The anti-fatigue composition of the present invention may be used as a raw material for medicines (including pharmaceuticals and quasi drugs). It can be produced by appropriately blending the anti-fatigue composition of the present invention with a raw material for a pharmaceutical preparation. Examples of the pharmaceutical raw material that can be blended in the anti-fatigue composition of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hydrogenated vegetable oil, kaolin, Talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar, carbonate) Sodium hydride, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (No. Quaternary ammonium base, sodium lauryl sulfate, etc. , Adsorbent (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

The administration method of the anti-fatigue composition according to the present invention can generally be administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids and the like. It may be administered parenterally. When administered as a parenteral agent, it can be applied in the form of a solution, or in the form of a haptic agent, lotion agent, ointment, tincture, cream, etc. with the addition of a dispersant, suspension, stabilizer, etc. can do.
The dosage may vary depending on the administration method, medical condition, age of patient, etc., but for adults, it can be generally administered as 0.5 to 1000 mg as an active ingredient per day, and for children, about 0.5 to 500 mg can be usually administered.
The compounding ratio of the anti-fatigue composition can be appropriately changed depending on the dosage form, but is usually about 0.3 to 15.0 wt% when administered orally or by mucosal absorption, when administered parenterally. Is preferably about 0.01 to 10 wt%. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range.

Example: Production of anti-fatigue composition (rice extract) Example 1
For germinated rice, sasanishiki rice is polished with a normal rice mill (for example, RMA-150 manufactured by Yanagisawa Seisakusho) to remove the koji, and then the germinated rice is further grounded by a grinding rice mill (for example, Satake Seisakusho, TM5 etc. ), And then the rice germ was separated from the endosperm-derived powder and straw using a suitable sieve (about 32 mesh).

After 200 g of raw rice germ prepared by the above method was stirred for 2 hours at 69 ° C. (reflux temperature), the lipid was extracted and separated once. After extraction, the defatted germ was filtered to remove n-hexane. Next, 8 ml of water adjusted to pH 5.6 was added to 0.5 g of the defatted germ obtained, and the mixture was shaken at 40 ° C. at 100 strokes / minute for 4 hours to obtain rice germ enriched in γ-aminobutyric acid.
Thereafter, the rice extract (Example 1) was obtained by subjecting the rice germ enriched with γ-aminobutyric acid to water extraction, concentration sterilization, and spray drying.

Example 2
For germinated rice, sasanishiki rice is polished with a normal rice mill (for example, RMA-150 manufactured by Yanagisawa Seisakusho) to remove the koji, and then the germinated rice is further grounded by a grinding rice mill (for example, Satake Seisakusho, TM5 etc. ), And then the rice germ was separated from the endosperm-derived powder and straw using a suitable sieve (about 32 mesh).

After 200 g of raw rice germ prepared by the above method was stirred for 2 hours at 69 ° C. (reflux temperature), the lipid was extracted and separated once. After extraction, the defatted germ was filtered to remove n-hexane. Next, 8 ml of water adjusted to pH 5.6 was added to 0.5 g of the defatted germ obtained, and the mixture was shaken at 40 ° C. at 100 strokes / minute for 4 hours to obtain rice germ enriched in γ-aminobutyric acid.
Thereafter, glutamic acid was added to the rice germ and fermented with lactic acid bacteria to further enrich γ-aminobutyric acid. Thereafter, the rice germ enriched in γ-aminobutyric acid was further extracted with water, concentrated, sterilized and spray-dried to obtain a rice extract (Example 2).
The contents of γ-aminobutyric acid and other components of Example 1 and Example 2 are as described in Table 1 below.

Test example: Evaluation of anti-fatigue function of Examples Preparation of mental fatigue-inducing reagent Reserpine (Wako Pure Chemical Industries, Ltd., hereinafter also referred to simply as RSP) 1.5 mg was weighed and dissolved in 10 mL of glacial acetic acid and diluted to 10 mL with distilled water (hereinafter referred to as “this”). In the description, it is referred to as “mental fatigue inducing reagent”).
A sample to be administered to the control group was prepared by diluting 10 mL of glacial acetic acid with distilled water to 10 mL (hereinafter referred to as “control reagent” in this specification).

2. Preparation of administration sample
50 mg of the rice extract of Example 1 dissolved in 10 mL of distilled water was prepared (hereinafter referred to as “Example 1-1 sample” in this specification).
Similarly, 100 mg of the rice extract of Example 1 dissolved in 10 mL of distilled water was prepared (hereinafter referred to as “Example 1-2 sample” in this specification).
Further, 50 mg of the rice extract of Example 2 dissolved in 10 mL of distilled water was prepared (hereinafter referred to as “Example 2-1 sample” in this specification).
100 mg of the rice extract of Example 2 dissolved in 10 mL of distilled water was prepared (hereinafter referred to as “Example 2-2 sample” in this specification).
For comparison, pure GABA manufactured by Kyowa Hakko was dissolved in 10 mL of distilled water (hereinafter referred to as “pure GABA sample” in this specification), and caffeine (manufactured by Wako Pure Chemical). Was dissolved in 10 mL of distilled water (hereinafter referred to as “caffeine sample”).

3. The grouping was made as follows.
(1) Control group (2) Comparative example 1 group: RSP group (3) Example 1-1 group: RSP and Example 1 were administered at 50 mg / kg (4) Example 1-2 group: RSP and Example (1) 100 mg / kg administration (5) Example 2-1 group: RSP and Example 2 administration 50 mg / kg (6) Example 2-2 group: RSP and Example 1 administration 100 mg / kg ( 7) Comparative Example 2 group: 100 mg: RSP and pure GABA administered at 50 mg / kg (8) Comparative Example 3 group: RSP and caffeine administered at 50 mg / kg

4). Test Method Example 1-1 Group First, the mental fatigue inducing reagent (1.5 mg / kg) was subcutaneously injected into mice to induce mental fatigue. 24 hours later, 0.1 mL of the above Example 1-1 sample was orally administered.
Further 24 hours later, Example 1-1 was orally administered. One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
Example 1-2 group It carried out by the same method except having used the said Example 1-2 sample as an administration sample.
Example 2-1 group It carried out by the same method except having used the said Example 2-1 sample as an administration sample.
Example 2-2 group It carried out by the same method except having used the said Example 2-2 sample as an administration sample.
Control group First, mice were subcutaneously injected with the Contro reagent (1.5 mg / kg). 24 hours later, 0.1 mL of distilled water was orally administered.
After another 24 hours, distilled water was orally administered. One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
Comparative Example 1 Group First, mice were injected subcutaneously with the mental fatigue inducing reagent (1.5 mg / kg). 24 hours later, 0.1 mL of distilled water was orally administered.
After another 24 hours, distilled water was orally administered. One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
Comparative Example 2 Group First, mice were subcutaneously injected with the above mental fatigue inducing reagent (1.5 mg / kg). After 24 hours, 0.1 mL of a pure GABA sample was orally administered.
Further 24 hours later, the pure GABA sample was orally administered. One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
Comparative Example 3 Group First, mice were injected subcutaneously with the above mental fatigue inducing reagent (1.5 mg / kg). 24 hours later, 0.1 mL of the above caffeine sample was orally administered.
After another 24 hours, the caffeine sample was orally administered. One hour later, forced swimming was performed for 10 minutes, and the degree of recovery from fatigue was evaluated by measuring the immobility time.
The results of the tests for each group are shown in FIG.

Test Results According to FIG. 1, the immobility time is longer in Comparative Example 1 than in Control. As a result, it was confirmed that the immobility time was prolonged by the administration of RSP, and mental fatigue was induced.
Furthermore, according to FIG. 1, compared with the comparative example 1, the immobility time is significantly shorter in the example 1-1, the example 1-2, the example 2-1, and the example 2-2. (Comparative Example 1: 424.1 seconds, Example 1-1: 373.8 seconds, Example 1-2: 335.6 seconds, Example 2-1: 382.9 seconds, Example 2-2: 385 seconds). Thereby, it turns out that the rice extract of Example 1 and Example 2 has the function to reduce mental fatigue.
In addition, compared to Comparative Example 2 (404.9 seconds) in which a pure GABA sample was administered, Example 1-1, Example 1-2, Example 2-1 and Example 2-2 were significantly more immobile. You can see that it is getting shorter. Thereby, it turns out that the rice extract (Examples 1 and 2) containing (gamma) -aminobutyric acid has the function to reduce mental fatigue rather than pure (gamma) -aminobutyric acid (comparative example 2). .
It can also be seen that the immobility time is shorter in Example 1-2 (Example 1: 100 mg sample) than in Example 1-1 (Example 1: 50 mg sample). Thus, it can be seen that the immobility time is shortened in a concentration-dependent manner by administering the rice extract of Example 1 to mice in which mental fatigue has been induced by RSP administration. It can be seen that the rice extract of Example 1 has a function of reducing mental fatigue in a concentration-dependent manner.
Furthermore, the immobility time is shorter in Example 1-1, Example 1-2, Example 2-1, and Example 2-2 than in Comparative Example 2 (404.9 seconds) in which a pure GABA sample was administered. You can see that Thereby, it is confirmed that the rice extract (Examples 1 and 2) containing γ-aminobutyric acid has a function of reducing mental fatigue than the pure γ-aminobutyric acid (Comparative Example 2). It was done. It can be seen that mental fatigue can be further reduced by containing both γ-aminobutyric acid and rice-derived components (proteins, lipids, carbohydrates, etc.).

Furthermore, according to FIG. 1, the immobility time is shorter when Example 1 is administered than when Example 2 is administered. Thereby, mental fatigue can be further reduced by containing moderate amounts of γ-aminobutyric acid and rice-derived components (peptides, lipids, carbohydrates, etc.).
In Comparative Example 3 (administration of caffeine sample), the immobility time was significantly reduced, which was shortened by about 125 seconds as compared with Comparative Example 1, and was shortened to the same level as that of the control. However, the mice administered with the caffeine sample moved abnormally as if excited after administration. Thus, it is considered that the immobility time was shortened by the excitatory action of caffeine rather than the fatigue recovery.

[Composition example]
The compounding example of the composition for anti-fatigue by this invention is shown. The blending of the anti-fatigue composition is not limited to the following blending examples.
Formulation Example 1: Chewing gum
53.0wt% sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Anti-fatigue composition 0.5
100.0wt%

Formulation Example 2: Gummy
Reduced water tank 40.0wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Orange juice 4.0
Yuzu Flavor 0.6
Dye 0.02
Anti-fatigue composition 1.0
100.0wt%

Formulation Example 3: Candy
Sugar 50.0wt%
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Anti-fatigue composition 0.4
100.0wt%

Formulation Example 4: Yogurt (hard / soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Anti-fatigue composition 0.4
Perfume
Water residue
100.0wt%

Formulation Example 5: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Anti-fatigue composition 0.3
Perfume
Purified water residue
100.0wt%

Formulation Example 6: Tablets
76.4 wt% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Anti-fatigue composition 0.5
Purified water 3.9
100.0wt%

Formulation Example 7: Soft capsule
Brown rice germ oil 47.0wt%
Yuzu extract 40.0
Emulsifier 12.0
Anti-fatigue composition 1.0
100.0wt%

Formulation Example 8: Tablet
Lactose 54.0wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Anti-fatigue composition 1.0
100.0wt%

Formulation Example 7: Coffee drink (liquid)
Roasted coffee beans 6.0wt%
Sugar 6.0
Baking soda 0.2
Emulsifier 0.15
Anti-fatigue composition 1.0
Water residue
100.0wt%

Formulation Example 8: Coffee drink (powder)
Instant coffee 90.0wt%
Skim milk 7.0
Anti-fatigue composition 3.0
100.0wt%

Formulation Example 8: Chocolate confectionery
Chocolate 95.0wt%
Nonfat milk 3.0
Sugar 1.0
Anti-fatigue composition 1.0
100.0wt%

  As described above, the anti-fatigue composition of the present invention has a function of particularly reducing mental fatigue by using a rice extract containing γ-aminobutyric acid derived from rice as an active ingredient, By containing both γ-aminobutyric acids and other rice-derived components (peptides, lipids, saccharides, etc.), mental fatigue can be further reduced. Furthermore, the anti-fatigue composition of the present invention is a composition derived from a natural edible plant and has no side effects and can be ingested over a long period of time. Therefore, the anti-fatigue composition of the present invention has a function of reducing fatigue, particularly mental fatigue, and can provide a safe food composition, pharmaceutical composition or cosmetic composition.

It is a graph which shows the immobility time of the mouse | mouth of the Example (rice extract) measured by the method by the said test example, and a comparative example (distilled water, pure GABA, etc.).

Claims (8)

Rice germ (A) extracted from at least one of rice germ, rice bran containing germ, germ rice, brown rice, germinated brown rice, and white rice as an active ingredient,
The anti-fatigue composition characterized in that the rice extract (A) contains γ-aminobutyric acid (a-1) derived from rice. The content of the rice-derived γ-aminobutyric acid (a-1) is 1 to 99% by mass in terms of γ-aminobutyric acid when the rice extract is 100% by mass. Item 2. The anti-fatigue composition according to Item 1. The anti-fatigue composition according to claim 1 or 2, wherein the rice extract (A) contains rice-derived peptides (a-2). The content (a-2) of the peptide is 0.1 to 35% by mass in terms of protein when the content of the rice extract is 100% by mass. The anti-fatigue composition according to any one of the above. The anti-fatigue composition according to any one of claims 1 to 4, wherein the rice extract (A) contains rice-derived saccharide (a-3). The anti-fatigue composition according to any one of claims 1 to 5, wherein the rice extract (A) contains a lipid (a-4) derived from rice. The anti-fatigue composition according to any one of claims 1 to 6, wherein the rice extract (A) contains dietary fiber (a-5) derived from rice. The anti-fatigue composition according to any one of claims 1 to 7, wherein the anti-fatigue composition further contains γ-aminobutyric acid (B).

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