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HUE031692T2 - Eljárások rovarfertõzések genetikai szabályozására növényekben, valamint ezek készítményei - Google Patents

Eljárások rovarfertõzések genetikai szabályozására növényekben, valamint ezek készítményei Download PDF

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HUE031692T2
HUE031692T2 HUE11191393A HUE11191393A HUE031692T2 HU E031692 T2 HUE031692 T2 HU E031692T2 HU E11191393 A HUE11191393 A HU E11191393A HU E11191393 A HUE11191393 A HU E11191393A HU E031692 T2 HUE031692 T2 HU E031692T2
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James A Baum
Claire A Cajacob
Pascale Feldmann
Gregory R Heck
Irene Nooren
Geert Plaetinck
Ty T Vaughn
Wendy Maddelein
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Monsanto Technology Llc
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Claims (8)

  1. Eljárások ro^arllrtOKisek genetikaivalamint erők készítményei SZABADALMI IGÉNYPONTOK
    1. INâmukfetiÇÂ választhatónk ki: (a) égy poiinukleotid. amely a 704. számú szekvenciával rendelkező nukíeinsav szekvenciát tartalmazza; (b) egy poinukleotkk amely az 5XSSC, 50% forrnarniÇ és 42 °C 10 percig mosási körök·, méuyek között libfidizaíddik á 704. számő szék ve nciaval rendeIkezd nukíeinsav szekvenek ához.; |é) polmnkleotid, amely legalább 70%-os szekvencia azonosságot mutat a 704, számú szekvenciával irendéikezo nokleinsav szekvenciával; (d) a 704. számú szekvenciává! rendelkező nukiei nsav szekvenciának egy leplabb 21 egy-bédiggo ntíkieotMbol állő firagmense, és (e) az (a). (bk#} vagy (d) poöt szerinti szekvencia komplementere, ahol a szóban .forgó poiinukleotid tnokődes szempontjából egy heterológ promoterhez kapcsolódik; és ahol ha egy fedelesszárnyú növényi; kártevő a szóban forgó polínukleotiddal vagy a szóban forgó Éagmenssel kompletnentér szálát tartalmazó kétszálú ribonulleotid legalább egyik szálát székvem Cüt lenyeli, az gátolja á szőhan íbrgó kártevő növekédését. Ê, Az 1. Igénypont szerinti poiínukleőtii, amely a definíció szerint egy növényi transzformációs vektorban található. %, Az 1. igénypont szerinti polmukleotid exprcsszllásával keletkező kétszálú ribonukleotid szekvencia, almi a szóban forgó ribonukieotid szekvenciának egy fodelésszarnyS nő" vény) kártevői által történő lenyelése gátolja a szóban forgó kártevőt. 4. A3, igénypont szerinti kétszálú ribonukleotid szekvencia, (i> amelyet a defimció szerint úgy állítunk elő, hogy készítünk egy rekombináns poiinukleotid szekvenciát, amely tartalmaz egy első, egy második és egy harmadik poiinukleotid ssfokvéncíák ahol az első polí:üukIiőllÍ;fykvfö«ciá tanaimig I, igénypont szerinti izolált polnukleotidöt, és ahol a barmadik polinokleotid szekvenÄ a második poiinukleotid szekvenoián keresztül kapcsolódik az első poiinukleotid szekvenciához, és ahol a harmadik poiinukleotid szekvencia lényegében az első poiinukleotid székvenciá mverz:kömpiementet:es öíy módon, hogy az első és a harmadik poiinukleotid szekvencia Mb- rldizálődik, amikor égy kiïszâlé flbonuMeotid mÂkiîàï ké pezve, amelyet a kapcsolódói»efil fihonukleötid szekvencia stabilizál: vagy (II) ahol a poiinulieoiid szekvenciának a kárteve? által történő lenyelése gátolja egy olyan nukleotid szekvencia expresszióját, amely lény egében konapiementer a szóban forgó pölínukleötid szekvenciával.
  2. 5. Az 1. igénypont szerinti polinukleotiddaí transzformált sejt. ő. Az 5. igénypont,szerint\ sejt, amely a defimció szerint (i) egy prokarióta sejt; vagy (íi) egy eukarióta sejt; vagy (iii) egy növényi vagy bakteriális sejt ?. Az !. vagy 2. igénypont szerinti polmukleotiddal transzformált növény, vagy annak magja, amely tartalmazza a polírrukleotidot. 8 A 7, igénypont szerinti növény, amelyben a szóban forgó poli nukleotid a növény egy sejtjében kétszéló rlbonpkleotld szekvenciaként evpresszákxlik, és a szóban forgó kéíszálú fibonuk-léotid szekvenciának egy rovarkánevöt gátló ípennyiségben történő lenyéiése ^y tápban gátolja a szóban forgó kártevők bogy tovább táplálkozzo».S2bb®0»íforgi táppal» előnyösen (i) a rovarkártevőt a következő csoponböl választhatjuk ki: Diabmtica virgißta, JMahmtic# wrgißm virgißm WïtibmËM v&g{ßm mi, Biahmtim è&Ëeàm, Bmbratíca bűphm, M&bmtim vóAMm Mtâwtmïspmimgi és B&miim tmdecmptmcîaki: vagy fiij a kétszáiú rtbonnideotid szekvencia rovarkártevőt gátló mennyiségének lenyelése gátolja a kártevő növekedéséi 9. A ?. igénypont szerinti nővlnyttol előállított árucikk, aboi a szóban forgó árucikk kimutatható mennyiségben tartalmaz egy; az ! igénypont szerinti polmukleötidrói expresszáh ríbonukieotidot.
  3. 10. Eljárás iédelesszámyú kártevő fertőzés gátlására, azzal jellemezve, hogy a fedeles-szárnytArovarkártevö táplálékában biztosítunk egy szert, amely tartaípaz egy első poiinukleotid szekvenciát;, amely a rovarkártevő által való lenyelés utátit igytműkfdfk;., $ mÿfcm ylőfe^;^ÉÍ''^M0Íógtaí funkciót, ahol a szóban forgó polmukleotid szekvencia körülbelül 95-100*13 azonosság# jputifc .l^#bb körülbelül 19-25 egybefüggő nukleotid mentén egy, a szóban forgó kártevőből szármázó kódoló szekvenciával, és hibridizálődik egy második ptakleotid szekvenciához, amely komplementer a szóban forgó első polínukleotid szekvenciával, és aböt a láziban fbrgó kártevőből: származó kódoló szekvencia a /0. számú, szekvencia, vagy annak Ismpietnenfere, 1 É A ife Igénypont szerinti; eljárás,. azzal jellemezve, hogy a szobán ίοrgő fedelesszárnyó kártmè ^ Dîaèmîim s0r> mnolyet ëonyosen a kővetkező csoportból választhatunk fel: Bigbmiim vtrgißm &ic$w!;m vir^gm wfgißm, Biaàmtim. virgißm zeö, Bikám fim èûfiemta, Biäbmiim búréern^ ÎJtabmüek víriátéá, jtbmbmtïm spmkmï,
  4. 12, Eljárás fedelesszárnyú kártevő fertőzés gátlására, azzal jellemezve, hogy a fedeles-szárnyé rovarkártevő táplálékában blzíosltunk egy nöyénysejiet, amely egy, m. I, Igénypont szeriní? püiiaúfcleőtk! szekveneíát ezpresszá| ahol a póiinubieötidot égy expresszáltattuk, bogy egy kétszálú rihonöklemsavat iectné|en, atnely a kártevő által történi lenyelés után úgy működik, begy a szobán forgó kártevőben gátolja egy megcélzott szekvencia ekpfessriöjáí,: ennek eredményéképpen kevésbé táplálkozik a szóban forgó táplálékból, mint egy olyan iápfáiélfeöl, amelyből hiányzik a növénysejt
  5. 13, A Í2. Igénypont szerinti eljárás,, ázzál, tetiemezve, bogy fi) a seit lenyelése után a kártevő csökkent növekedést mutat; vagy («) a növénysejt tartalmaz még egy poiinukleotid szekvenciái, amely égy rovarirtó azért kő* dob amelyet a kővetkező csoportból választhatunk ki: egy pataím, egy Bacillus thurmgmms rovarirtó fehérjé, egy XéMtíéúhátm. rovarirtó: fehérje, egy PkmkrhabBm rovarirtó fehérje, egy Pizcillus iiá&amp;rospúrus rovarirtó fehérje, és egy Bad Bus sphaericm rovarirtó fehérje, a szóban forgó Bmiikm ihumigi&amp;mís rovarirtó fehérjét előnyösen a következő csoportból váiasZfbaljdk ki: égy Eryl. egy C’ry3,mgy OTIM, egy CryÍT70, egy Cry22, egy TIC90Î, egy TIC20Í, egy T1C407, egy TlCAVl, egy bináris rovarirtó fehérje £ryET22 és €ryËT34, égy bináris rovarirtó fehérje CryEtlIÖ és CryET76, egy bináris rovarirtó fehérje TfCiOO és TIC101, az ET29 vagy ET3? rovarirtó fehérje kombinációja a T1C810 vagy T1C8I2 rovarirtó fehérjékkel, és egy bináris rovarirtó fehérje FS149BÍ; vagy (in) a megcélzott szekveneia egy Mtérjét kódok amelynek a várható áínkeiójáf a kővetkezők közül válasmhatjuk ki; izomképzŐdéSj juvenilis hormon képződés, jnvenilis hormon szabályozás, ionszabályozás és - transzport, emésztő enzim szintézis, i Sejtmembrán potenolil fenntartása, ami-nosav bioszintézis, aminoaav lebomlás; spermaképződés, férőmön szintézis, férőmön érzékelés, tapogatok képződése, szárnyképzödés, lábképződés» fejlődés és differenciálódás,, peteképzodés, lárva érés, emésztő enzim képződés, bémoümfa szintézis, hemolimfa fenntartás, neuroiransmnisszió, sejtosztódás, energia metaboilznms, légzés és apoptózis; vagy (iv) a szóban forgo fedeiesszámyó kártevő egy Duxbrotíca φρ·, amelyet előnyösen a kovert kézö csoportból váÍMZÍbatunk ki: Biabroüca virgifem, Diabrotica dsgtfem vlrgifem, BiPimtick virgpcm $m< Biábmílpp -balteaia, Diabroúca barbert, Biabrotíca virkluh, DiabmPca ψ&amp;<Μ$α, és IMührotím knéecímpímclúis:p vagy (v) a kártevő; által történő lenyelés után a pöiíttokléöid %y működik, hogy egy, a rovar tÉlë» léséhez; lényeges gént szupprésszái, a szóban forgó funkciót á kivetkezők közül választhatjuk ki: a kártevő fapiálközása, a kártevő seit apoptézisa, a sejt dlITerenciálódása és fejlődése, a szexuális reprodukcióra való képesség vagy vágy, izomképződék izomrángás, izom összehúzódás, juvenilis hormon képződés^ juvenilis hormon szabályozás, íonszáhllyozÉs és -drahszport, a sejtmembrán potenciál fenntartása, aminosav bioszintézis, aminosav lebomlás, spermaképzödés, férőmön szintézis, ferotnon érzékelés, tapogatók képződési, szÉsiyiépződés, iábképzödés, pefeképződés, lárva érés, emésztő enzim képződés, hémolimía szirttéÂ, hemoiimfa fenntartás, ueuroíranszmisszió, tárva állapotba történő átmenet, bébábozédás, a bábáilapotbói való kiemelkedés, sejtosztódás, energia liá» izmus, légzés és a eiîôszkeiëtâlis szerkezet kialakulása,
  6. 14, Eljárás egy rovarkártevő fertőzésnek kitett haszonnövényből előállított termés- hoza-mának a jávilasárá., azzal jellemezve, hogy a szóban forgó eljárás a következő lépéseket tartalmazza: aj egy 1. igénypontszerini bejuttatunk a bj a haszonnövényt tenyésztjük, hogy lehetővé tegyük a szobán forgó polmukieotid expresszióját, ahol a szóban forgó polinukleotid expressziója gátolja a növény rovarkártevők által történő fogyasztását, és a kártevő fertőzés miatti hozamcsökkenésé 15, A 14 Igénypont szerinti ehárás, azzal jellemezve, hogy (i) a polinukleotid éxpressztcp egy RNS molekulát, termek amely elnyom legalább egy első megcélzott gént a rovarkártevőben, amely lenyelte a szóban forgó haszonnövény egy részép ahol a megcélzott génnek legalább egy lényeges fonkdőja van, amely a következők közül választható ki. a kártevő táplálkozásán á. kártevő életkepessége, a kártevő sejt apoptózi&amp;a, á kártevő vagy bármelyik kártevő sejt differenciálódása és fejlődése, a kártevő szexuális réprődúkciója, izomklpződéá, izom» rángás, izom összehúzódás, juvemllS: fmramn képződés és/vagy csökkentés.,: juvenilis hormon azabáv lyozás, ionsznbályozás és -dranszport, a sejtmembrán potenciál fenntartása, amltmsav bioszinfézis.; aminosav febontlil spetfeaképzőclls* feromon szintézis, feromon érzékelés, tapogatok képződése, szárnyképződés, lábképződés, peíeképzödés, lárva érés, emésztő éstzim képződés, hemolimia szinté» zis; hemolímía fenntáríás, neurofranszmisszio, lárva állapotba történő átmehet, bebábözödás, a bábák lappiból való kiemelkedés, séftöszfoclás, energia metaboiizmus, légzés, a cifószkeietáíis szerkezet szintézise és fenntartása, pukleotid metáboizmus., nitrogén metaboiizmus, vízfelhasználás, vízvisszatartás, és érzékszervi észlelés; vagy (iij a rovarkártevő égy gabona gyökérfeeg kártevő, amelyet a következők közül választhatónk ki: Diabroíim wideeímpmemfa howardí {Déli kukorica gyökérféreg (SCRjk Diabrotiea vtrgifem vfrgißm (Amerikai kuhericabogár (W£R}| Di&amp;bmtfm barbmi (Északi kukorica gyokérferég: (NCR)}, Diabroiiva virgißra zea (Mexikói kukorica gyökérféreg plflÍSjj; Diabrotíca babeaia (BfMi kukorica gyökérféreg (BZR)X Diahmüca viriéila (Brazil kukorica gyökérféreg íBZR)}, és MiäBmMM· spmBm (Brazil kukonca gyökér féreg (BZR>)
  7. 16, Híjára* égy rovarkártevő fertőzésnek kitett haszonnövényből előállított termény szá-Mzsagdoleraneiáiànak a javítására, akKsljellcsneaye. bogy a azőian forgo eljárás a következő lépé~ sekel taríá»M: a) a aÄanfeiO hrann«^ í. igénypont szerinti poikukieoikfot, b) a Immmmvim tenyésztjük, bogy lehetővé tegyük a szóban forgó pohniikleotid mpmwélfa, ahol a polínukfemid expresszsöja gátolja, hogy a rovarkártevők a haszomtövenyből táplálkozzanak, valamint a S2érâzsàgûr#tiéÂÂ:Ârtsy^::.fe#^: miatti
  8. 17, Eljárás egy árucikk, éÂâlpab m0 .$zzitj; jflogy előállí tunk egy 7, Igénypont szerinti növényt, vagy annak «gy részét, és a növényből, vagy annak egy részéből élőálltjuk az árucikket, élelmiszert vagy tápot, IS. A 17, igénypont szerűm eljárás, azzal jellemezve, hogy az élelmiszer vagy a táp lehet olaj, liszt, felérje, keményítő, liszt vagy szilázs.
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