GB1425918A - Process for enzyme purification - Google Patents
Process for enzyme purificationInfo
- Publication number
- GB1425918A GB1425918A GB4153872A GB4153872A GB1425918A GB 1425918 A GB1425918 A GB 1425918A GB 4153872 A GB4153872 A GB 4153872A GB 4153872 A GB4153872 A GB 4153872A GB 1425918 A GB1425918 A GB 1425918A
- Authority
- GB
- United Kingdom
- Prior art keywords
- lyase
- groups
- cross
- dextran gel
- active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
1425918 Hyaluronidases BIOREX LABORATORIES Ltd 22 Oct 1973 [7 Sept 1972] 41538/72 Heading C3H Glucuronoglycosaminoglycan hyaluronate lyase is obtained in very pure and highly active form by first contacting the crude lyase with a cross-linked polyacrylic divinyl-benzene resin containing carboxyl groups as active groups, eluting the adsorbed lyase from the resin, dialysing the eluate fractions rich in the lyase against a saturated solution of ammonium sulphate and further purifying the resulting enzyme concentrate by adsorption of the enzyme on, and elution from, a cross-linked dextran gel. A dextran gel containing active carboxymethyl groups is preferably employed, particularly in the final stage of purification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4153872A GB1425918A (en) | 1972-09-07 | 1972-09-07 | Process for enzyme purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4153872A GB1425918A (en) | 1972-09-07 | 1972-09-07 | Process for enzyme purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1425918A true GB1425918A (en) | 1976-02-25 |
Family
ID=10420160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4153872A Expired GB1425918A (en) | 1972-09-07 | 1972-09-07 | Process for enzyme purification |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1425918A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4152212A (en) * | 1976-02-25 | 1979-05-01 | Biorex Laboratories Limited | Process for the purification of glucuronoglycosaminoglycan hyaluronate lyase |
| FR2495634A1 (en) * | 1980-12-08 | 1982-06-11 | Biorex Laboratories Ltd | ENZYME NEW AND USEFUL IN THERAPEUTICS |
-
1972
- 1972-09-07 GB GB4153872A patent/GB1425918A/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4152212A (en) * | 1976-02-25 | 1979-05-01 | Biorex Laboratories Limited | Process for the purification of glucuronoglycosaminoglycan hyaluronate lyase |
| FR2495634A1 (en) * | 1980-12-08 | 1982-06-11 | Biorex Laboratories Ltd | ENZYME NEW AND USEFUL IN THERAPEUTICS |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |