GB894151A - Preparation of high purity streptokinase - Google Patents
Preparation of high purity streptokinaseInfo
- Publication number
- GB894151A GB894151A GB486/59A GB48659A GB894151A GB 894151 A GB894151 A GB 894151A GB 486/59 A GB486/59 A GB 486/59A GB 48659 A GB48659 A GB 48659A GB 894151 A GB894151 A GB 894151A
- Authority
- GB
- United Kingdom
- Prior art keywords
- streptokinase
- phosphate buffer
- purified
- treatment
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3153—Streptokinase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to a high purity streptokinase effective for the treatment of thrombosis which is an amorphous solid, soluble in water and having a molecular weight of about 47,000, a sedimentation rate constant of about 3.0 x 10-13, a diffusion constant of about 6.20 and 10-7 and an electrophoretic mobility (m ) of 4.19 x 10-5cm2sec-1volt-1, the preecding physical properties being measured in phosphate buffer at pH 7.5 and an ionic strength of 0.15. Crude streptokinase is adsorbed on an ionizable cellulose-type chromotographic column, desorbing purified streptokinase therefrom by means of gradient elution by means of a phosphate buffer having a molarity of 0.1 to 0.005 M, having a pH of 5.8 to 8.5 and a temperature of 0 DEG C. to 10 DEG C., recovering the streptokinase from the eluate by treatment with conventional precipitating agents for streptokinase, adsorbing said purified streptokinase on a second ionizable cellulose type chromatographic column, desorbing further purified streptokinase from said second column by means of gradient elution using the aforesaid phosphate buffer and recovering said further purified streptokinase from the eluate by conventional precipitating agents for streptokinase. Specifications 714,803, 715,103, 715,110, 715,572 and 715,930 are referred to.ALSO:An intravenous solution effective for the treatment of thrombosis comprises an aqueous solution of a purified streptokinase prepared by adsorption on a cellulose anion exchanger and elution with a phosphate buffer (see Group IV (b)). Preservatives, colouring-agents and other non-therapeutic injectable substances may be present. 50,000 to 100,000 units of streptokinase per hour may be administered.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US894151XA | 1958-01-29 | 1958-01-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB894151A true GB894151A (en) | 1962-04-18 |
Family
ID=22217505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB486/59A Expired GB894151A (en) | 1958-01-29 | 1959-01-06 | Preparation of high purity streptokinase |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB894151A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2121685A (en) * | 1982-04-30 | 1984-01-04 | Kaken Pharma Co Ltd | Protease compositions for treating blood lipids abnormality and arteriosclerosis |
-
1959
- 1959-01-06 GB GB486/59A patent/GB894151A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2121685A (en) * | 1982-04-30 | 1984-01-04 | Kaken Pharma Co Ltd | Protease compositions for treating blood lipids abnormality and arteriosclerosis |
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